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58results about How to "Fast growth" patented technology

SNP molecular marker for growth traits of litopenaeus vannamei, detection primer, kit and application thereof

The application discloses a SNP molecular marker of Litopenaeus vannamei growth traits, a detection primer, a kit and application thereof. The SNP molecular marker is shown as SEQ ID NO. 1, and the polymorphism of the base at the 497th position from the 5' end of SEQ ID NO. 1 is A / G; the SNP molecular marker can simultaneously screen body length and weight to carry out Litopenaeus vannamei breeding, and the SNP molecular marker can also be detected by using a primer designed according to the sequence of SEQ ID NO. 1 or prepared into a kit. Meanwhile, the AA genotype of the SNP molecular marker Chr25_11409188 provided in the application can be used to select Litopenaeus vannamei parents, further improve the growth speed of the body length and weight of a breeding population, provide an innovative tool for molecular breeding of Litopenaeus vannamei, shorten a breeding cycle, and help industrial development and efficiency improvement.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA +1

A high-protein biological fermentation type aquatic feed and its preparation method

PendingCN122074602AImprove digestion utilizationbright body colorClimate change adaptationAnimal feeding stuffBiotechnologyAquatic animal
The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.
Owner:HAINAN CHIEF BIOLOGY TECHN EMPOLDER CO LTD

Electrode Continuous Flow Experimental Apparatus

ActiveCN224430367Ufast growthimprove accuracy
This invention discloses a continuous flow electrode experimental device, comprising: a reaction chamber, an air bladder, a current module, a circulation pipe, and an aeration module. The reaction chamber includes a sealed detection chamber containing a detection solution. The air bladder is connected to the top of the detection chamber. The current module is electrically connected to the detection solution, forming an electrical circuit. The current module includes electrode plates disposed within the detection solution. The circulation pipe is connected to the detection chamber, forming a circulation loop for circulating the detection solution. The aeration module is disposed in the circulation pipe and includes an aeration membrane. This continuous flow electrode experimental device can improve the success rate of experiments and is applicable to the field of wastewater treatment.
Owner:JIANGMEN POLYTECHNIC

A method of constructing a fast-growing sterile zebrafish model

ActiveCN120555443Bfast growthGonadal dysplasia is infertileHydrolasesClimate change adaptationWild typeEconomic benefits
This invention discloses a method for constructing a fast-growing, sterile zebrafish model, belonging to the field of fish animal model preparation technology. This invention is the first to discover the role of the CK gene in regulating fish growth rate and fertility: fish with simultaneous knockout of ck1 and ck2 genes exhibit rapid growth and gonadal abortion. Using the method provided by this invention, a fast-growing, sterile zebrafish model was successfully constructed. These zebrafish exhibited gonadal dysplasia and sterility, but also rapid growth. Compared to wild-type zebrafish, the 4-month-old double mutant zebrafish showed significantly increased body length and weight. The disclosed gene function and zebrafish model construction method can be used for breeding fast-growing, sterile fish varieties and studying related mechanisms, which is of great significance for improving the economic benefits of aquaculture and protecting ecological security.
Owner:HUAZHONG AGRI UNIV

A device for treating acrylonitrile wastewater

The present application relates to sewage treatment technical field, specifically to a kind of acrylonitrile sewage treatment device, comprising: microbial purification tank, exhaust mechanism, the exhaust mechanism has two and symmetrically set in the two sides of the inner wall of microbial purification tank width direction, the exhaust mechanism includes the cavity plate fixedly connected in the inner wall of microbial purification tank. By cavity plate, partition block and air separation assembly in exhaust mechanism, air can be injected and extracted into microbial purification tank, so as to provide corresponding purification environment for aerobic microorganism or anaerobic microorganism to purify sewage, and then realize that when injecting air, air exposure area and immersion area can inject air into microbial purification tank simultaneously to provide sufficient oxygen for aerobic microorganism to purify sewage, help its efficient decomposition of pollutants in sewage, while when extracting air, air separation assembly disconnects the communication of air exposure area and immersion area, so that suction airflow is only discharged from air exposure area, avoid sewage from flowing out with cavity plate, while avoiding suction airflow from immersion area to interfere with anaerobic environment in tank.
Owner:JIANGSU OCEAN UNIV

Gallium oxide epitaxial wafer and method for manufacturing the same

PendingCN122279739AInhibit lateral two-dimensional diffusionpromote growthPhysical chemistryHydrogen chloride
This invention relates to the field of semiconductor technology, specifically disclosing a gallium oxide epitaxial wafer and its preparation method. The preparation method of the gallium oxide epitaxial wafer includes the following steps: S1, placing a (011)-plane gallium oxide substrate in a reaction chamber, introducing a carrier gas, and then introducing oxygen, chlorine, and hydrogen for a first growth; S2, introducing oxygen and hydrogen chloride for a second growth on the first-grown gallium oxide substrate to obtain a gallium oxide epitaxial wafer; wherein, in S1, the flow rate ratio of oxygen to chlorine is (2~3):1; in S2, the flow rate of oxygen is 200~400 sccm, and the flow rate ratio of oxygen to hydrogen chloride is (5~20):1. This invention optimizes the number of growth cycles and growth conditions during the preparation of the gallium oxide epitaxial wafer, reduces epitaxial defect density, improves epitaxial film quality, and realizes the preparation of high-thickness gallium oxide epitaxial wafers with low surface defects and nanoscale surfaces.
Owner:CHINA ELECTRONICS TECH GRP NO 46 RES INST

Application of bacillus H8 in cultivation of emilia sonchifolia

PendingCN121975684ACharacteristics are clear and stablegood genetic stabilityBacteriaMicroorganism based processesBiotechnologyMedicinal herbs
The invention belongs to the technical field of plant cultivation, provides application of bacillus H8 in cultivation of emilia sonchifolia, and particularly relates to bacillus H8, a bacillus H8 suspension with the concentration of 102-104 CFU / mL is prepared and applied to the position 2-3 cm away from the root of emilia sonchifolia as a bio-fertilizer once every 7 days, plant growth can be remarkably promoted, and the yield of emilia sonchifolia is increased. The content of medicinal components of chlorogenic acid and quercitrin in the leaves is increased; the method solves the problems of soil degradation, unstable medicinal material quality and the like caused by dependence on chemical fertilizers and pesticides in traditional cultivation, is suitable for green and efficient cultivation of emilia sonchifolia, and has the advantages of simplicity and convenience in operation, environment friendliness, low cost and the like.
Owner:LIUPANSHUI NORMAL UNIV +1

Actate-resistant domesticated algal strain HpAc50 and application thereof

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.
Owner:ANHUI POLYTECHNIC UNIV

Method for improving the ability of broiler chickens to resist lps stress

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.
Owner:WUHAN SUNHY BIOLOGICAL +1

Crystal growth device and raw material recovery method

Embodiments of the present specification provide a crystal growth apparatus and a raw material recovery method, the apparatus comprising: a crucible for accommodating a crystal growth raw material; at least part of the seed crystal support can be immersed in a melt formed by the crystal growth raw materials in the crucible; the seed crystal support comprises a seed crystal bonding surface; the first supporting structure is connected with one side, opposite to the seed crystal bonding surface, of the seed crystal holder, and the first supporting structure is configured to drive the seed crystal holder to rotate. The first supporting structure is arranged to drive the seed crystal holder in the crucible to rotate, so that the mass transfer rate is increased, and the crystal growth speed is increased; meanwhile, the rotation of the seed crystal support can also improve the radial solute distribution uniformity of the seed crystal bonding surface and improve the growth quality of the crystal.
Owner:MEISHAN BOYA ADVANCED MATERIALS CO LTD

A method for segmented farming of golden pompano

ActiveCN120615787Bpromote rapid growthimprove immunity
The present application belongs to the technical field of aquaculture, and particularly relates to a segmented type of pompano breeding method. The segmented type of breeding method provided by the present application supplies nutrition accurately according to the requirements of different growth stages of pompano, and significantly improves the growth speed. Through the optimization of water temperature and light conditions in different stages, the growth speed of pompano is significantly accelerated, thereby shortening the breeding cycle. The addition of licorice powder and melatonin in the feed further enhances the immunity of pompano and reduces the use of drugs. Through the segmented type of breeding, the present application improves the unit yield and survival rate of pompano, and significantly improves the economic benefits.
Owner:HAINAN SHENNONG AQUATIC PRODUCTS SEED SOURCE TECHNOLOGY CO LTD

A lotus planting pond

This utility model discloses a lotus planting pond, including a planting pond body and a water storage tank. Multiple partitions are fixed inside the planting pond body, each partition having a drainage structure. A water pump is installed between the planting pond body and the water storage tank to pump water from the storage tank into the planting pond body. A water outlet structure is provided on one side of the planting pond body, including outlet pipe one, outlet pipe two, and outlet pipe three. This application, through the planting pond body, partitions, drainage structure, and water outlet structure, allows for corresponding adjustments to the water level in the planting pond body at different growth stages of the lotus, ensuring optimal water levels for lotus growth during the seedling, growth, and flowering stages. This helps guarantee the survival rate and growth vigor of the lotus at different stages, resulting in better lotus growth and enhanced ornamental value.
Owner:HUBEI MENGXIN XIANGPENG AGRICULTURAL DEVELOPMENT CO LTD

High-temperature-resistant thermal response type retarder as well as preparation method and application thereof

The invention discloses a high-temperature-resistant thermal response type retarder which is prepared by the following steps: firstly, polymerizing 3.5 to 5.5 parts of acryloyl glycinamide, 0.05 to 0.1 part of bisacrylamide monomer and 0.5 to 2.5 parts of acrylic acid monomer, and then polymerizing with 28 to 32 parts of 2-acrylamido-2-methylpropanesulfonic acid, 7 to 9 parts of acrylic acid monomer and 3 to 7 parts of acrylamide monomer. The invention also discloses a preparation method thereof. The high-temperature-resistant thermal response type retarder provided by the invention has relatively good high-temperature stability; carboxylate ions can be released in a high-temperature environment, so that delayed coagulation in the high-temperature environment is realized; and when the temperature is reduced, the released carboxylate ions are recycled, so that the strength of the set cement is rapidly developed in a medium-temperature environment.
Owner:CNPC BOHAI DRILLING ENG +1

Water cooling screen and single crystal furnace

ActiveCN224148223UObserve liquid levelObserving solid-liquid critical interfacePolycrystalline material growthBy pulling from meltThermodynamicsCooling effect
The utility model discloses a water cooling screen and a single crystal furnace. In the water cooling screen, an annular cooling water channel is formed in the body, and the water inlet channel and the water outlet channel are both communicated with the annular cooling water channel of the body; an annular cooling water channel is formed in the additional cooling cavity; the water inlet channel and the water outlet channel are also communicated with the annular cooling water channel of the additional cooling cavity; the additional cooling cavity and the body extend in the same direction, a bottom opening of the additional cooling cavity is located above a top opening of the body, and the outer diameter of the additional cooling cavity is smaller than the inner diameter of the top of the body. The cooling effect of the water cooling screen is improved, and the equal-diameter growth rate of the single crystal furnace is improved.
Owner:双良硅材料(包头)有限公司

Meychek yeast with amine-reducing and flavor-enhancing ability and application thereof

PendingCN122128118AHas complete degradationachieve degradationFungiMicroorganism based processesVolatile flavorMicrobiology
This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.
Owner:SICHUAN UNIV

Butterfly roll edge mushroom ZJXM002 strain and application thereof

PendingCN122234951Ameet needsEnrich market supplyFungiMicroorganism based processes
This application discloses a *Pleurotus ostreatus* strain ZJXM002 and its applications, relating to the field of microbial cultivation technology. The *Pleurotus ostreatus* strain ZJXM002 has the accession number CGMCC No. 41786; its classification is named... From Lep cremeoinvoluta This strain is suitable for artificial cultivation and can produce fruiting bodies of the Creamy Curly Mushroom ZJXM002 strain with high biological efficiency.
Owner:KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP

Application of rhizobium in promoting growth nodulation and nitrogen fixation of indigofera pseudotinctoria

PendingCN121950589AIncrease nitrogenase activityhigh activityBiocideAgriculture tools and machinesRhizobium sp.Nitrogenase
The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.
Owner:CHONGQING ACAD OF ANIMAL SCI

Exhaust light guide device

ActiveCN224121079UImprove total pressure efficiencyRain and snow resistantLighting applicationsMechanical apparatusLight guideControl circuit
The utility model provides an exhaust light guide device which comprises an exhaust assembly, a light guide assembly, a solar photovoltaic panel, an electric exhauster or a lighting lamp and the like, the exhaust assembly comprises an air guide exhaust cover and a ventilation light guide pipe, and the light guide assembly comprises a lighting protective cover and a ventilation light guide pipe. The air guide exhaust hood and the ventilation light guide pipe have a flow guide effect on incoming flow and generate negative pressure exhaust at the exhaust port; when sunlight, wind or no wind exists, the lighting protective cover and the ventilation light guide pipe can guide external light into a room, and meanwhile the solar photovoltaic panel generates electricity and drives the electric exhaust fan to exhaust air or supply energy to the illuminating lamp through the control circuit. The device can utilize natural wind power, solar energy and indoor hot air buoyancy to exhaust or illuminate, airflow does not flow backwards, and the device is simple in structure, high in wind resistance, low in cost, long in service life, low in noise and high in exhaust efficiency. The device is used for places where structures need exhausting, lighting or ventilation and heat dissipation.
Owner:YILI SHUNTONG (SHANDONG) ENERGY SAVING & ENVIRONMENTAL PROTECTION TECH CO LTD

Method for raising seedlings of cubilose fruits by grafting

The invention discloses a cubilose fruit grafting seedling raising method, and belongs to the technical field of fruit tree cultivation. The method comprises six aspects of stock planting field establishment, stock seedling raising, stock water and fertilizer management, grafting, grafted seedling water and fertilizer management and grafted seedling field management. A special cutter tool for cubilose fruit grafting is adopted, an L-shaped section can be formed, the section is smooth and good in integrating degree, and the grafting survival rate and the grafting efficiency can be improved. According to the invention, the seedling raising grids are used for stock seedling raising, so that the seedling raising cost can be saved, and the space utilization rate can be improved. According to the method, the thorn-free huanglong and the argentodoxa cuneata are adopted as the stocks, the root systems are developed, the morbidity of root-knot nematodes can be reduced, the grafting survival rate and the growth vigor are improved, and meanwhile the canker resistance of plants is improved; the quality of the rootstock is guaranteed, the grafting growth vigor is improved, bud germination is promoted, and fruit bearing is carried out in advance by optimizing the length, the period and the number of bud points of the rootstock.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

Ganoderma boninense strain gt1 and application thereof

PendingCN122278643Afast growthget out soonBiotechnologyMicrobiology
This invention belongs to the field of edible fungi technology, and particularly relates to a sessile Ganoderma lucidum strain GT1 and its applications. A sessile Ganoderma lucidum strain ( Ganodermasessile GT1, deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 2026405. This invention utilizes perennial, high-yield fungal grass to replace sawdust in the cultivation of edible and medicinal fungi, and screens suitable fungal grass formulas for Ganoderma lucidum cultivation, achieving high yield and high quality of Ganoderma lucidum, which is of great significance for the sustainable development of the Ganoderma lucidum industry. This invention optimizes the growth environment and substrate selection to achieve large-scale cultivation of edible and medicinal fungi, providing resource and technical support for in-depth research and application.
Owner:FUJIAN AGRI & FORESTRY UNIV

A method and device for preparing monocrystalline molybdenum disulfide at wafer level by MOCVD

The application discloses a kind of MOCVD preparation method and device of wafer level single crystal molybdenum disulfide;The preparation method uses six carbonyl molybdenum Mo (CO) 6 and liquid carbon disulfide CS2 as molybdenum source and sulfur source respectively, different from traditional MOCVD growth process, precursor used in the application does not contain hydrogen element, avoid the side effect caused by hydrogen element, and increase the pre-reaction of source cracking, by introducing oxygen, generate intermediate product molybdenum trioxide (MoO3) and sulfur element (S) in pre-reaction chamber, to the reaction chamber on the substrate surface deposition, effectively avoid the introduction of carbon impurities, realize the nucleation and epitaxial growth of single crystal orientation on the substrate surface, obtain high uniformity and continuity single crystal molybdenum disulfide film, realize 6 inch and above size single crystal wafer scale preparation, meet the key needs of high density, high performance electronic device integration.
Owner:SUZHOU LABORATORY +1

Methods for extracting β-nicotinamide mononucleotide from microalgae and microalgae extracts

This disclosure relates to a method for extracting β-nicotinamide mononucleotide from microalgae and the microalgae extract. The method includes: mixing microalgae raw material with an extractant for extraction; wherein the microalgae raw material is selected from one or more of Chlorella, Spirulina, and Haematococcus pluvialis; the extractant comprises 0.05–1.5 wt% of a nonionic surfactant, 0.5–1.0 wt% of an organic acid, 20–40 wt% of an alcohol, and the balance being a solvent. Microalgae raw materials are easy to cultivate, grow rapidly, have high protein content, and high β-nicotinamide mononucleotide content; the extraction method disclosed herein is simple, has a high extraction rate, and is low in cost.
Owner:CHINA PETROLEUM & CHEMICAL CORP +1

Lactobacillus plantarum from the deep sea of the South China Sea and application thereof in bioconversion of curcumin

This invention provides a strain of *Lactiplantibacillus plantarum* OLp4 from the deep sea of ​​the South China Sea. This strain was deposited on February 28, 2024, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), located in Guangzhou, with accession number GDMCCNo: 64378. This invention also provides an application of this *Lactiplantibacillus* strain in converting curcumin to tetrahydrocurcumin. The *Lactiplantibacillus* strain of this invention is easy to culture, grows rapidly, has strong acid-producing capacity, and exhibits extremely strong acid resistance.
Owner:NINGBO XINUOYA MARINE BIOTECH CO LTD

A type of Klebsiella variegata and its applications

ActiveCN117343864BThe cultivation method is simplefast growthBacteriaMicroorganism based processesBiotechnologyMicroorganism
This invention belongs to the field of environmental microbiology, specifically a type of Klebsiella variicola and its applications. Klebsiella variicola, strain IFPBD3, is deposited at the China General Microbiological Culture Collection Center (CGMCC) on May 25, 2023, with accession number CGMCC No. 27461. The rice straw degrading bacteria produced by the technical solution of this invention can effectively degrade rice straw, significantly increase straw fertility, and improve straw utilization rate.
Owner:SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI

Recombinant Escherichia coli strains, their preparation methods and applications, and the synthesis method of oryzanol A.

ActiveCN120699860Bfast growthshort fermentation cycle
This invention discloses a recombinant *Escherichia coli* strain, its preparation method and application, and a method for synthesizing oryzanol A, relating to the field of bioengineering technology. The recombinant *E. coli* strain uses *E. coli* BL21(DE3) as the chassis cell and undergoes the following modifications (a) and (b): (a) knocking out the pfkA gene; or, knocking out the pfkA gene and mutating the 5′ untranslated region of the gltA gene from the sequence shown in SEQ ID NO: 1 to the sequence shown in SEQ ID NO: 2; (b) inserting exogenous genes agmA, agmB, agmC, agmE, and agmF. This recombinant *E. coli* strain exhibits rapid growth, a short fermentation cycle (within 72 hours), can utilize inexpensive and readily available culture media, and significantly increases the fermentation yield of oryzanol A compared to existing *E. coli* systems.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI

A method for cultivating seedlings directly from the germination of *Gynostemma pentaphyllum* seeds in Yunnan.

ActiveCN121464934BSolve the problem of large-scale plantingeasy to operatePlant tissue cultureHorticulture methodsRoot growthCymbidium goeringii
This application discloses a method for directly cultivating seedlings from the germination of *Cymbidium goeringii* seeds in Yunnan. The method includes the following steps: sowing *Cymbidium goeringii* seeds on a modified culture medium, followed by 6 months of constant temperature cultivation at 25℃ to obtain small pseudobulbs suitable for direct transplanting. This method is simple to operate; seeds begin to germinate and turn green 20 days after sowing, begin to grow leaves after 45 days, begin to root after 60 days, and show significant root growth after 90 days. The pseudobulbs also enlarge synchronously. After 6 months, the plants naturally shed their leaves in the tissue culture bottle, and the pseudobulbs can be transplanted to the field for acclimatization and cultivation. This method eliminates the need to change the culture medium, removes the manual transfer step in the tissue culture process, and solves the problems of yellowing, difficult rooting, and high contamination rates after transfer of tissue culture seedlings. Furthermore, the plants naturally shed their leaves in the tissue culture bottle, directly obtaining small pseudobulbs without hardening off, and improving the transplant survival rate, which is beneficial for the large-scale cultivation of *Cymbidium goeringii*.
Owner:KUNMING INST OF BOTANY CHINESE ACAD OF SCI +1

High-strength flame-retardant environment-friendly packaging corrugated paper

ActiveCN224213045UProtective dispersion supportEffectively disperse and disperse support forceBio-packagingSpecial papercardboardStarch
The utility model discloses high-strength flame-retardant environment-friendly packaging corrugated paper which comprises a corrugated paper inner core, arc-shaped grooves are evenly formed in the surface of the corrugated paper inner core, and cylindrical inner cores are fixedly connected into the arc-shaped grooves. The upper surface and the lower surface of the corrugated paper inner core are fixedly connected with protective paperboards. According to the high-strength flame-retardant environment-friendly packaging corrugated paper, the corrugated paper inner core is a main supporting layer of the corrugated paper, the arc-shaped groove wrinkle structure formed in the corrugated paper inner core can effectively disperse vibration and impact force of packaging materials in the transportation process, so that the packaging materials are protected against damage, the cylindrical inner core can effectively disperse supporting force on the corrugated paper inner core, and the packaging materials are not prone to damage. The arc on one side of the protective paperboard is attached to the surfaces of the cylindrical inner core and the corrugated paper inner core to protect the cylindrical inner core and the corrugated paper inner core, external impact force is effectively reduced, a starch-based material can be selected and used, the material can be naturally decomposed within a certain time, and garbage landfill is reduced.
Owner:TANGSHAN JIUBO TECHNOLOGY CO LTD

A recombinant expression vector and engineering bacteria of pertussis antigen and application thereof

The application provides a pertussis antigen recombinant expression vector and engineering bacteria and application thereof. The pertussis antigen recombinant expression vector comprises a resistance screening gene, an upstream recombinant nucleic acid fragment of a pertussis exotoxin gene and a downstream recombinant nucleic acid fragment, wherein the upstream and downstream recombinant nucleic acid fragments can respectively perform homologous recombination with the upstream and downstream of the pertussis exotoxin gene. The application takes the pertussis bacillus as a host, constructs an engineering bacteria ΔPT strain for producing FHA and PRN by knocking out the PT gene, simplifies the production process of the FHA and PRN antigens, maintains the high purity and immunogenicity of the FHA and PRN antigens, eliminates the interference of the PT protein in the production of the FHA and PRN antigens, avoids the PT protein detoxification process, and eliminates the risk of possible PT protein toxicity reversal.
Owner:JIANGSU THERAVAC BIO PHARMA CO LTD

Recombinant rhizobium OEuspA with stress resistance, plant salt-resistant microbial inoculum and application of plant salt-resistant microbial inoculum

The invention discloses recombinant rhizobium OEuspA with stress resistance, a plant salt-resistant bacterial agent and application of the plant salt-resistant bacterial agent, and belongs to the technical field of genetic engineering. According to the invention, a new uspA gene is identified from azotobacter stenoma ORS571 for the first time. And then carrying the uspA gene into the azotobacter stem nodule by using a plasmid vector, and constructing to obtain the recombinant rhizobium over-expressing the uspA gene. Compared with wild-type rhizobium, the stress resistance of the recombinant rhizobium for overexpressing the uspA gene, which is constructed by the invention, is remarkably improved. After the recombinant rhizobium over-expressing the uspA gene is symbiotic with sesbania eriocalyx, wheat and corn, the growth of plants under the salt stress condition can be promoted. The uspA gene disclosed by the invention can be used for constructing a recombinant rhizobium material with stronger stress resistance and growth promotion capability, and has potential and wide application prospects in the fields of genetic engineering transformation of rhizobium or other bacteria, the rhizobium microbial inoculum preparation industry, the bean planting industry in agricultural production and saline-alkali soil development.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Series of compounds methyltrifluoroborate and nonlinear optical crystal of methyltrifluoroborate and preparation method and use thereof

The present application relates to a series of compounds methyl trifluoroborate and methyl trifluoroborate nonlinear optical crystal and preparation method and use, the molecular general formula of the series of compounds is A3[CH3BF3]X2, wherein A=NH4, K, Rb, Cs, X=[NO3], Cl, Br, the molecular weight is 207.86-641.37, top seed method is prepared, orthorhombic system, space group Cmc 21 (No.36), the cell parameter is a=11.9141 (17)-12.6254 (2) Å, b=8.1556 (12)-8.4654 (17) Å, c=10.9621 (14)-11.5625 (15) Å, α =90, β =90, γ =90, Z =4, V=1065.2 (3)-1261.9 (1) Å 3 The series of compounds methyl trifluoroborate nonlinear optical crystal obtained by the method has the advantages of wide light transmission waveband, stable physical and chemical properties, moderate mechanical hardness, not easy to break, easy to cut, polish processing and preservation and the like.
Owner:XINJIANG TECH INST OF PHYSICS & CHEM CHINESE ACAD OF SCI