This invention discloses a
hoof protein product and its preparation method, belonging to the field of
component separation and extraction; using
hoof as
raw material, the method includes the following steps: (1)
hoof pretreatment: the hoof is washed, crushed and sieved to obtain hoof
powder (2) subcritical extraction: the hoof
powder is taken and pure water containing food-grade acid-base
regulator is added, and a reaction solution is prepared by subcritical
hydrolysis reaction (3) the reaction solution is centrifuged, separated, dialyzed and dried to obtain hoof
protein; the extracted hoof
protein is rich in total essential amino acids (more than 40 g / 100g), among which the content of essential amino acids such as
histidine,
leucine,
isoleucine,
threonine and
cysteine exceeds the content of ideal protein amino acids recommended by WHO / FAO, and has high nutritional value. The
essential amino acid index (EAAI),
biological value (BV) and nutritional index (NI) are close to the
gold standard of
egg protein, and the digestibility is high. It has good functional characteristics and has the potential to be a
protein supplement component; the preparation process of this invention is scientific and reasonable, easy to operate, low in cost, can realize
continuous production, and has no
pollution to the environment.