106 results about "Influenza virus Antigen" patented technology

The present invention relates to providing new vaccines and treatments for the diseases related to canine influenza virus. It discloses influenza viral antigens, and methods of presenting these antigens to canines, especially dogs. It relates to attenuated and killed vaccines. The present invention relates to experimentally generated canine and equine influenza viruses. The invention also includes influenza A, including H3, N8, H3N8, H7N7 and viruses which contain at least one genome segment from an canine or equine influenza virus. The present invention also relates to the use of these viruses in therapeutic compositions to protect canines, dogs in particular, from diseases caused by influenza viruses.

The present invention provides an immunogenic influenza composition in a dose volume suitable for human use, comprising an influenza virus antigen or antigenic preparation thereof and an adjuvant composition comprising an oil-in-water emulsion, wherein said oil-in-water emulsion comprises a metabolisable oil at a level of below 11 mg and an emulsifying agent at a level of below 5 mg and optionally a tocol or a sterol at a level of below 12 mg. Suitably the amount of influenza antigen per strain per dose is 15 μg HA or a low amount such as less than 15 μg HA.

Compositions, fusion proteins and polypeptides comprise at least one pathogen-associated molecular pattern and at least a portion of at least one integral membrane protein of an influenza viral antigen. The compositions, fusion proteins and polypeptides are used to stimulate an immune response in a subject.

The invention seeks to avoid components in split vaccines that could cause an excessive Th2 response. Thus the invention provides an immunogenic composition comprising a split influenza virus antigen and a Th1 adjuvant, wherein the antigen is preferably prepared from a virus grown in cell culture (e.g., it is free from egg proteins).

Influenza vaccines containing insoluble particulate adjuvants have been found to elicit an IgG response that is primarily a TH2 response (IgG1). This response can be shifted towards a TH1 response (IgG2a) by including immunopotentiators in the compositions. Thus the invention provides an immunogenic composition comprising: (i) an influenza virus antigen; (ii) an insoluble particulate adjuvant; and (iii) a immunopotentiator.

The invention discloses a human anti-H7N9 avian influenza virus neutralizing antibody 1F7L screened by a single cell sorting technology. The amino acid sequences in the light and heavy chain variable regions are shown in the formulas of SEQ ID No. 2 and SEQ ID No. 5. The antibody has the ability to neutralize the H7N9 influenza viruses in vitro and mediates the killing (ADCC) of the H7N9 influenza virus-infected cells with effector cells mainly comprising NK cells. The antibody can be used as a treatment drug for highly pathogenic avian influenza infection and can also be used for the development of H7N9 influenza virus antigen detection reagents.

Influenza vaccines with oil-in-water emulsion adjuvants are known. The amount of emulsion adjuvant required for an influenza vaccine can be reduced, thereby allowing more vaccines to be made from a given amount of emulsion, and / or minimizing the amount of emulsion that has to be produced for a given number of vaccine doses. These vaccines can conveniently be made by mixing (i) an oil-in-water emulsion and (ii) an aqueous preparation of an influenza virus antigen. In one aspect, substantially equal volumes of components (i) and (ii) are used; in another aspect, an excess volume of component (ii) is used. When using substantially equal volumes, component (ii) has a hemagglutinin concentration of more than 60 μg influenza virus strain per ml. Components (i) and (ii) can be presented in kit form.

While oil-in-water emulsions are excellent adjuvants for influenza vaccines, their efficacy can be improved by additionally including other immunostimulating agent(s) to improve cytokine responses, such as γ-interferon response. Thus, a vaccine comprises (i) an influenza virus antigen; (ii) an oil-in-water emulsion adjuvant; and (iii) a cytokine-inducing agent.

The invention supplies a rapid testing tape that could simultaneously test A, B virus antigen. It covers FluA-McAb, FluB-McAb and IgG on NC film, compounds FluA-McAb, FluB-McAb marked by colloidal gold, simultaneously tests A, B virus antigen in tested sample by using film chromatography double antibody cream filling method. The invention could decrease cockamamie process, and could gain result in 10-15 minutes. It is suitable to hospital and the research for large scale epidemiology to handle sporadic affair.

The invention discloses a novel coronavirus antigen and influenza virus antigen combined detection reagent strip which comprises a bottom plate. A sample pad, a gold-labeled pad, a nitrocellulose membrane and absorbent paper are sequentially pasted on the bottom plate, and the surface of the nitrocellulose membrane is sequentially coated with an anti-novel coronavirus antibody, an anti-influenza Avirus antibody and an anti-influenza B virus antibody. A rabbit anti-mouse IgG antibody coats the end close to the absorbent paper. A colloidal gold labeled anti-novel coronavirus antibody, an anti-influenza A virus antibody and an anti-influenza B virus antibody are sprayed on the gold-labeled pad. The binding protein A / G is marked on the surface of the colloidal gold, the protein A / G is specifically bound with the Fc end of the antibody, so that the ideal conformation of Fab end abduction is constructed, and meanwhile, the binding of the protein A / G with the Fc can reduce the false positiveinterference of rheumatoid factors on a detection structure. The reagent strip can simultaneously realize qualitative detection of the novel coronavirus antigen and the influenza antigen in one test,and is convenient to use, good in sensitivity, high in specificity and short in detection time.

The invention discloses neutralizing monoclonal antibodies for resisting the H7N9 influenza virus and particularly discloses two monoclonal antibodies aiming at the H7N9 influenza virus. The antibodies has evident combining activity to H7N9 influenza virus antigens.

The present disclosure generally relates to a composition comprising one or more peptides selected from influenza virus antigenic peptides M2e, HA0, BM2 and a M2e-BM2 fusion peptide in a composition with a cationic liposome delivery vehicle, and the use of these compositions as a universal vaccine against influenza A and / or B viral strains.

The invention provides an influenza virus splitting vaccine and a preparation method thereof. Each dose of influenza virus splitting vaccine contains three influenza hemagglutinins, namely H1N1, H3N2 and type B, a CpG ODN adjuvant and an aluminium adjuvant. The invention also provides a preparation method for the influenza vaccine, which comprises the following steps of: multiplying influenza viruses in chick embryo; performing ultrafitration and concentration; centrifuging and purifying; splitting; performing secondary purification; inactivating; and diluting and packaging. The influenza virus splitting vaccine can reduce the dosage of the influenza virus antigen and production cost of the influenza vaccine, and is suitable for quickly improving influenza vaccine supplying capacity under the threat of global influenza pandemic.

The invention discloses a humanized anti-H7N9 avian influenza virus high-affinity antibody 10K filtered and obtained based on a single cell separation technology, the amino acid sequences of light chain and heavy chain variable regions of the antibody are shown as SEQ ID No. 2 and SEQ ID No. 5 respectively. The high-affinity specificity of the antibody is combined with H7N9 avian influenza virus 7 type hemagglutinin protein, and can mediate the kill and wound (ADCC) of effector cells using NK cells as main parts for H7N9 influenza virus infected cells. The antibody 10K can be used for therapeutic development of highly pathogenic avian influenza infection, and also can be used for development of H7N9 influenza virus antigen dectection reagents.

The invention discloses a chicken egg-yolk antibody capable of resisting influenza virus. The chicken egg-yolk antibody is used for specifically resisting tetravalence influenza virus. The influenza virus includes influenza A virus subtype H1N1, influenza A virus subtype H3N2, influenza B virus subtype Victoria series and influenza A virus subtype Yamagata series. The preparation method of the chicken egg-yolk antibody comprises the following steps of 1, preparing a tetravalence influenza virus antigen; 2, performing immunity on hens; 3, collecting immunized eggs laid by the immunized hens; 4, preparing a mixed antibody stock solution from the immunized eggs; 5, detecting titer of the antibody stock solution, and a stabilizer is added cooperatively, so that the chicken egg-yolk antibody capable of resisting tetravalence influenza virus is obtained. The chicken egg-yolk antibody can be combined with the tetravalence influenza virus specificity pathogen for a short period of time, the tetravalence influenza virus can be effectively killed for a long period of time, the safety performance is good, no toxic or side effect is generated, and the chicken egg-yolk antibody can be effectively used for preventing pathogen corresponding to human pharyngeal and nasopharynx mucosas.

Methods of making compositions that stimulate a protective immune response in a subject include a portion of a protein from a naturally occurring viral hemagglutinin, wherein the portion includes at least a portion of a globular head, includes at least a portion of at least one secondary structure that causes the globular head to essentially retain its tertiary structure, and lacks a membrane fusion domain, a transmembrane domain and a cytoplasmic domain. Compositions comprise a flagellin component or a Toll-like Receptor agonist component that is at least a portion of a flagellin or a Toll-like Receptor agonist, wherein the flagellin component or Toll-like Receptor agonist component includes at least one cysteine residue and whereby the flagellin component or Toll-like Receptor agonist component activates a Toll-like Receptor 5 or Toll-like Receptor. Compositions comprise a flagellin component that is at least a portion of a flagellin, wherein at least one lysine of the flagellin component has been substituted with at least one arginine, serine and histidine, whereby the flagellin component activates Toll-like Receptor 5. Compositions can further include an antigen, such as an influenza antigen, a flavivirus antigen, a pathogen-related antigen, a bacterial capsular antigen and a carrier protein. The compositions are used to stimulate an immune response and a protective immune response in a subject.

The invention provides a method for preparing nasal-spray type influenza virus vaccine containing CpG ODN and poly I:G adjuvant, which belongs to the technical field of biology. The method comprises the following steps: adopting chicken embryo to culture proliferative influenza virus; obtaining purified influenza virus through ultrafiltration concentration and column chromatography; obtaining influenza vaccine stock solution through lysis; and proportioning the influenza vaccine stock solution of a required type to CpG ODN adjuvant or I:G adjuvant. The invention provides the method for preparing novel nasal-spray type influenza virus vaccine, which can reduce the amount of influenza virus antigen, provides convenience for immunization and vaccination, and is suitable for rapidly improving the capacity of supplying influenza vaccine under threat of global pandemic influenza.

This invention disclose a kind of equipment used to detect the fowl influenza virus antibody correctly, fast, sensitively, with high specificity and economically. The equipment includes immunity sensor, enzyme labeled double antibody reaction system, electrolytic cell and data collection and process system; the immunity sensor includes the electrode system which is composed with the work electrode, the reference electrode and the assistant electrode which are printed in the insulated base plate; the electrolytic cell includes the detection base liquid, the beater and the temperature controller; the data collection and process system includes the electrochemistry workstation, the computer and the software system; the immunity sensor connects the electrochemistry workstation by lead; the work electrode of the immunity sensor is carbon electrode, which has coated the gold size induction film embed fowl influenza virus antibody. This invention also discloses a kind of method to use the equipment to detect the fowl influenza virus antibody.

Antigen and adjuvant components of an adjuvanted influenza vaccine are not mixed during manufacture, but are provided as separate components for extemporaneous mixing at the time of use, for example as a kit comprising (i) an antigen component, comprising an influenza virus antigen; and (ii) an adjuvant component, comprising an aluminium salt.

Antibodies (Abs) play roles in protection against influenza. Neutralizing Abs either inhibit the binding of hemagglutinin (HA) to cellular receptors or prevent the conformational change of HA induced by low pH. The former Ab binds to the regions near the sialic acid-binding pocket on the globular head formed by HA1 and generally shows narrow strain specificity. The latter Ab binds to the stem region formed mainly by HA2 and shows broad strain specificity. We isolated a broadly neutralizing Ab against H3N2 viruses. X-ray analysis of the HA / Ab complex indicated that the Ab binds to the valley formed by two neighboring HA monomers at the side of the globular head. The Ab shows neutralizing activity by preventing the conformational change of HA induced at low pH.

The invention belongs to the field of bioinformatics, and discloses a method for predicting changes of influenza virus antigens. The method comprises the following steps of: firstly, coding an influenza virus sequence pair aiming at the analysis characteristics of the influenza virus and the influenza virus antigen change; secondly, automatically extracting main characteristics of antigenic changeon the influenza virus pair by using a depth neural network, the influenza virus pair is then predicted for antigen change based on the extracted characteristics.

The invention concerns a recombinant modified vaccinia virus Ankara (MVA virus) expressing at least two external influenza virus antigens and / or an epitope of one or more of the at least two antigens and at least two internal influenza virus antigens and / or an epitope of the at least two antigens. The invention, thus, concerns a recombinant MVA virus encoding multiple external and / or internal influenza virus antigens, preferably from multiple influenza virus strains. The invention further concerns the use of said recombinant MVA in preparing a medicament and vaccine for influenza virus. Further encompassed by the present invention are methods, composition and kits.

The invention provides recombinant rhinovirus vectors including, for example, influenza virus antigens. Also provided by the invention are corresponding pharmaceutical compositions and methods.

The present invention relates to a pharmaceutical vaccine composition comprising: (a) a pathogen-derived antigen selected from the group consisting of Mycobacterium tuberculosis antigen, Bacillus anthracis antigen, HAV (hepatitis A virus) antigen, HBV (hepatitis B virus) antigen, HCV (hepatitis C virus) antigen, HIV (human immunodeficiency virus) antigen, influenza virus antigen, HSV (herpes simplex virus) antigen, Hib (Haemophilus influenzae type b) antigen, Neisseria meningitidis antigen, Corynebacterium diphtheriae antigen, Bordetella pertussis antigen, Clostridium tetani antigen and Varicella virus antigen; (b) a deacylated non-toxic LOS (lipooligosaccharide); and (c) a pharmaceutically acceptable carrier.

The invention relates to a liposome influenza virus antigen vaccine and a preparing method thereof. The liposome influenza virus antigen vaccine is prepared with a film evaporation method. The preparing method includes the steps of preparing an influenza virus antigen vaccine stock solution, preparing empty liposome, synthesizing liposome and the like. Compared with a linear epitope vaccine, the liposome influenza virus antigen vaccine prepared with the preparing method of the liposome influenza virus antigen vaccine has the advantages that multiple or multiple kinds of antigens can be carried at the same time, various cellular immune responses can be induced, and high immune responses can be caused; in addition, the liposome influenza virus antigen vaccine is stable in performance and long in storage life, and meanwhile the use immunogen dosage can be reduced.