8 results about "Bordetella" patented technology

The application discloses a traditional Chinese medicine composition for preventing and treating chicken Bordetella, a preparation method and application thereof. The traditional Chinese medicine composition for preventing and treating chicken Bordetella comprises the following raw medicinal materials in parts by weight: one red 15-20 parts, Tianwen grass 15-20 parts, goldenrod 10-15 parts, Jixiang grass 10-15 parts, white yam 5-12 parts, Buddha's hand 5-12 parts and platycodon 5-10 parts. The traditional Chinese medicine composition adopts one red, Tianwen grass, goldenrod, Jixiang grass, white yam, Buddha's hand and platycodon for reasonable compounding, has the effects of clearing heat and cooling blood, relieving cough and asthma, and can effectively treat chicken Bordetella disease. The seven kinds of medicines are mixed to prepare an oral liquid, the seven kinds of medicines have a synergistic effect in the preparation process, the effects of the medicines are effectively exerted, and the effects of clearing heat and detoxifying, clearing heat and cooling blood, relieving cough and asthma are further enhanced. The composition is scientifically matched, has no obvious toxic side reaction and drug tolerance, and can provide a train of thought for solving the problem of antibiotic resistance.

This invention discloses an engineered strain of Bordetella pertussis, its preparation method, and its application. The engineered Bordetella pertussis strain and the Bordetella pertussis strain (…) Bordetella pertussis Compared to ), expressing Jinzhou Pseudomonas ( Pseudomonas jinjuensis The lpxD protein is derived from [source missing]. The strain of Bordetella pertussis engineered strain provided by this invention has a significant effect on increasing OMV production, which will promote the development of Bordetella pertussis OMV vaccines.

The invention provides a primer for detecting bordetella pertussis through RPA (recombinase polymerase amplification)-lateral flow immunochromatography. The primer comprises specific primer pairs NAT-F and NAT-R which are designed aiming at transposase genes of a bordetella pertussis IS1663 family, and specific primer pairs NAT-F and NAT-R which are designed aiming at transposase genes of a bordetella pertussis IS1663 family, the invention also provides corresponding probes, which comprise a gold nanoparticle binding probe, a quality control line capture probe and a bordetella pertussis IS1663 capture probe. Meanwhile, the invention further provides application of the primer and the probe in preparation of a pertussis bordetella detection product, a specific detection kit and a specific detection method. According to the invention, the specific primer and probe are designed aiming at the transposase gene of the pertussis bordetella IS110-like element IS1663 family, and the rapid, high-sensitivity and high-specificity detection of pertussis bordetella is realized by fusing the rapid isothermal amplification technology of RPA and the visual detection advantage of lateral flow immunochromatography, no complex instrument or professional operation is needed, and the kit has the advantages of high sensitivity, high specificity, high sensitivity and high specificity. The method is suitable for multi-scene early convenient diagnosis.

The invention belongs to the technical field of biological medicines and vaccines, and particularly relates to a pertussis Bordetella mRNA (messenger Ribonucleic Acid) vaccine and application thereof. Specifically, the bordetella pertussis mRNA vaccine capable of inducing an organism to generate TH1 immune bias is successfully prepared, three antigens (pertussis toxin, filamentous hemagglutinin and pertussis adhesion) of pertussis are coded by mRNA based on the immune response advantage of the mRNA vaccine, so that the organism can be stimulated to generate good TH1 / TH17 immune response, and the immune response of the organism is enhanced. And thus, colonization and removal of the respiratory tract of the pertussis bacteria are realized, and finally, the protective efficacy of the vaccine is greatly improved, so that the method has good practical application value.

This invention discloses a method for preparing and applying a Pasteurella-Bordetella nanovaccine, belonging to the field of biomedical technology. The method includes the following steps: emulsifying epigallocatechin gallate, an antigen aqueous phase, and soybean oil under high-speed shearing, followed by homogenization to form a stable oil-in-water emulsion, which is the Pasteurella-Bordetella nanovaccine. Challenge experiments confirmed that the ESO vaccine achieved a protection rate of up to 90% against Pasteurella multocida and Bordetella bronchiseptica, significantly superior to the 40%-70% protection rate of the aluminum adjuvant group. Simultaneously, it significantly reduced the bacterial load in the lungs, demonstrating excellent clinical protective effects. This invention, through the innovative combination of EGCG and vegetable oil, overcomes the limitations of traditional adjuvants in terms of immunogenicity, safety, and single function, providing a highly efficient and safe new vaccine solution for the prevention and control of Pasteurella multocida and Bordetella multocida infections.

The present invention relates to processes for Bordetella fermentation and virulence factor production, especially PT production, for large scale manufacturing. More particularly, processes including a medium conditioning step carried out prior to inoculation.

The application discloses a Burkholderia glumae RS-36 and application thereof, and belongs to the technical field of microorganisms. Burkholderia gladioli The application provides a Burkholderia glumae (B. glumae) RS-36 with a preservation number of CCTCC No: M 20221584, and application of the B. glumae RS-36. Burkholderia gladioli The B. glumae RS-36 provided by the application has inhibiting effects on Magnaporthe oryzae, Ustilaginoidea virens, Exserohilum turcicum and Pyricularia grisea, can secrete indole acetic acid for promoting plant growth, can keep good activity in an acidic environment with a pH of 5 and a drought environment, has strong stress resistance, and has important significance for promoting green and sustainable development agriculture.

The invention provides a method for determining the purity of pertussis bordetella adhesin protein through an SEC-HPLC method, and belongs to the technical field of pertussis bordetella detection. After the pertussis bordetella adhesin protein is diluted into a sample solution, SEC-HPLC is adopted for determination, and the purity of the pertussis bordetella adhesin protein is obtained. Wherein a mobile phase adopted by the SEC-HPLC is a phosphate buffer solution II containing sodium chloride, the pH value of the phosphate buffer solution II is 6.5-7.5, the elution mode is isocratic elution, and the flow velocity of the mobile phase is 0.3-1 mL / min; and the column temperature of the size exclusion chromatographic column is 20-30 DEG C. Compared with an SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method, the SEC-HPLC method for detecting the purity of the pertussis Bordetella adhesin protein has the characteristics of simplicity in operation, high sensitivity, good repeatability and high intermediate precision when being used for detecting the purity of the pertussis Bordetella adhesin protein.