93 results about "Bordetella pertussis" patented technology

The invention belongs to the field of protein separating purification and relates to an industrialized large-scale separating purification method of 69KD outer membrane protein of pertussis bacillus. The method comprises extracting pertussis bacillus CS thallus in buffer solution with potential of hydrogen (pH) 8.8 and containing sodium chloride of 1mol/L to 2mol/l for thirty minutes to three hours at 40 to 60 DEG C, performing centrifugation, collecting supernatant, and obtaining thallus lysate supernatant of the 69KD outer membrane protein; b adding ammonium sulfate into the thallus lysate supernatant of the 69KD outer membrane protein to enable the ammonium sulfate to achieve 15% weight/volume to 25% weight/volume, standing the solution for one hour to eight hours, performing centrifugation, and collecting the supernatant; c adding ammonium sulfate into the supernatant collected in step b to enable the ammonium sulfate to achieve 30% weight/volume to 38% weight/volume, standing the solution for one hour to eight hours, performing centrifugation, abandoning the supernatant, and collecting deposit; d dissolving the deposit collected in the step c through Tris-HCl buffer solution, and dialyzing the solution to remove the ammonium sulfate; and e enabling the solution to pass through a diethyl aminoethyl (DEAE) sepharose ion exchange column, utilizing buffer solution with linear gradient sodium chloride concentration from 0 mol/L to 1 mol/L for washing, and collecting objective protein. The purification method for the 69KD outer membrane protein of the pertussis bacillus can fast and conveniently purify natural conformation 69 KD outer membrane protein so that the method can be applied to large-scale industrial preparation of the natural configuration 69 KD outer membrane protein.