148 results about "Staphylococcus aureus" patented technology

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

The invention belongs to the technical field of oral care, and discloses a rhodiola rosea-containing composite antibacterial oral bacteriostatic paste, which is prepared by compounding rhodiola rosea, tea polyphenol and other five-compound antibacterial components with sorbitol and other auxiliary materials according to an accurate proportion. According to the invention, a triple system of'instant sterilization-lasting bacteriostasis-ecological restoration 'is constructed, third-party detection shows that the inhibition rate on oral pathogens such as escherichia coli, staphylococcus aureus and the like reaches 99.9% or above, and multiple effects of resisting bacteria, diminishing inflammation, protecting gingiva, preventing caries and the like are achieved. The problems that an existing product is single in antibacterial effect, narrow in antibacterial spectrum and low in rhodiola rosea utilization rate are solved, no heavy metal is detected, stability is excellent, irritation is low, and the rhodiola rosea antibacterial agent is suitable for long-term use and has remarkable innovativeness and industrial value.

We provide new monoclonal antibody inhibitors of coagulases, staphylocoagulase (“SC”) and von Willebrand factor-binding protein (“vWbp”) for treatment of S. aureus. The monoclonal antibodies are useful in targeting the SC N-terminus of SC and vWbp (respectively) and inhibiting prothrombin activation. The monoclonal antibodies are able to bind to and interfere with, modulate, and / or inhibit the binding interactions between the coagulase protein and its ligan protein prothrombin the activation of prothrombin.

This invention discloses a method for preparing a chitosan antibacterial film. First, berberine aqueous solution and tannic acid aqueous solution are prepared separately. Then, the berberine aqueous solution is added dropwise to the tannic acid aqueous solution to form a mixed solution. The mixed solution is stirred and heated to obtain a suspension. The suspension is freeze-dried to obtain berberine-tannic acid nanoparticles. Next, a chitosan acetic acid aqueous solution is mixed with the berberine-tannic acid nanoparticles, and a film is formed to obtain a chitosan antibacterial film loaded with berberine-tannic acid nanoparticles. The prepared chitosan antibacterial film has low water content and swelling degree, strong ultraviolet shielding ability, high transparency, and strong inhibitory activity against Staphylococcus aureus and Salmonella typhimurium, which can better meet the needs of food preservation.

The application belongs to the technical field of microorganisms and particularly relates to a Bifidobacterium adolescentis beneficial to skin, a derivative of the Bifidobacterium adolescentis and application of the Bifidobacterium adolescentis and the derivative. The derivative of the Bifidobacterium adolescentis has significant antioxidant and anti-inflammatory effects, can inhibit pathogenic bacteria of acne and other common skin infections such as Propionibacterium acnes, Staphylococcus aureus and Malassezia furfur, can eliminate pathogenic bacteria, can inhibit skin lipid accumulation, can avoid follicle blockage, and can improve skin problems in multiple directions, especially acne skin problems.

The application discloses a silver / schiff base antibacterial material, a preparation method and application, and relates to the field of antibacterial materials.The silver / schiff base antibacterial material, the preparation method and the application are characterized in that, based on the large electron cloud density of the carbon-nitrogen double bond of the schiff base, silver ions in a silver nitrate solution are uniformly adsorbed on a schiff base base through the electrostatic adsorption principle; under the reduction of a reducing agent, the silver ions are reduced to nanosilver ions, and a silver / schiff base antibacterial material is obtained, in which nanosilver ions in the form of nanospheres are uniformly dispersed on the schiff base in the form of a thin sheet layer, thereby greatly improving the dispersibility of the nanosilver ions.The preparation method is simple, low in cost, and capable of batch production, and has no cytotoxicity, and has high antibacterial effects on streptococcus, pseudomonas aeruginosa and staphylococcus aureus, and has a good application prospect.

The invention discloses an antibacterial hydrogel dressing taking spunlace non-woven fabric as a carrier and a preparation method of the antibacterial hydrogel dressing, and relates to the technical field of biomedical polymers. The antibacterial hydrogel dressing is composed of a spunlace non-woven fabric carrier layer and an antibacterial hydrogel functional layer loaded on the surface and in pores of the spunlace non-woven fabric carrier layer, and the preparation method comprises the following steps: sterilizing spunlace non-woven fabric to obtain a spunlace non-woven fabric carrier; adding the antibacterial component into the aqueous solution of the hydrophilic polymer base material to form a hydrogel precursor solution; and immersing the spunlace non-woven fabric carrier into the hydrogel precursor solution, and curing to obtain the antibacterial hydrogel dressing. The antibacterial hydrogel dressing prepared by the preparation method disclosed by the invention has the flexible breathable characteristic of spunlace non-woven fabric and the high swelling and antibacterial functions of hydrogel, has an excellent bactericidal effect on escherichia coli and staphylococcus aureus, can effectively promote healing of infected wounds, is suitable for clinical nursing of burn and traumatic skin injuries, and has wide application prospects. Wide clinical application prospects are realized.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

Described herein is a synergistic combination comprising a quorum-sensing inhibitor and / or postbiotic metabolite and an antibiotic. Typically, the postbiotic metabolite comprises at least one peptide. Related compositions, uses, and methods are also described, including methods for resensitizing resistant bacteria to an antibiotic, and methods of treating antibiotic-resistant infections, such as methicillin-resistant Staphylococcus aureus (MRSA).

The invention discloses a method for rapidly and efficiently preparing a series of novel ester group facaplysin derivatives by combining EDCI and HOBT to react with carboxyl facaplysin and directly carrying out ester exchange reaction on an active ester intermediate and alcohol without separation. The invention also discloses application of the derivatives in resisting methicillin-resistant staphylococcus aureus and escherichia coli. The preparation method comprises the following steps that EDCI and HOBT are combined to activate carboxyl facaplysin for reaction, an activated ester intermediate is generated, the activated ester intermediate and alcohol are directly subjected to esterification reaction without separation, and a series of ester-based facaplysin derivatives are obtained. According to the preparation method, ester-based Fascaplysin derivatives with different structures can be rapidly and efficiently constructed, and compared with a traditional method that ester-based carboline derivatives are firstly synthesized and then high-temperature intramolecular cyclization is performed, the time and the raw material cost are greatly saved, the synthesis efficiency is improved, the reaction process is observable and controllable, and the yield is stable. And the synthesized ester group facaplysin derivative has excellent antibacterial activity on methicillin-resistant staphylococcus aureus and escherichia coli.

Methods of treating preventing and / or substantially reducing a Pseudomonas aeruginosa (PA) infection and / or a Staphylococcus aureus (SA) infection by administration of a bivalent protein subunit-based vaccine are described. The vaccine can protect subjects with a prior history of exposure to SA from a new exposure to PA or SA, including methicillin resistant SA (MRSA), as well as being able to protecting with no prior exposure to a SA infection. Vaccine formulations are also described.

This invention relates to the field of plant extract application technology, particularly to banana fruit extract and its applications. This invention reveals that banana fruit extract exhibits excellent performance in relieving inflammation, anti-oxidation, scavenging free radicals, delaying cell aging, inhibiting viruses and bacteria (inactivating HPV, Staphylococcus aureus, Candida albicans, Escherichia coli, and Propionibacterium acnes), reducing infection, promoting cell regeneration and repair, promoting collagen synthesis, enhancing elasticity and firmness, and acting as a plant-derived immunomodulator. Furthermore, it serves as a natural, intelligent transdermal carrier. Safe and non-irritating, this extraction method has shown excellent application value in high-value plants such as saffron, rose, Dendrobium officinale, ginseng, and Ganoderma lucidum. It provides a comprehensive solution for skin from defense to regeneration, offering a regulatory-compliant natural solution for functional skincare.

The invention relates to the technical field of microorganisms, in particular to a culture medium for staphylococcus aureus phage fermentation and a fermentation method. According to the culture medium for staphylococcus aureus bacteriophage fermentation, the problems that in large-scale culture of staphylococcus aureus bacteriophages, excessive accumulation of host bacteria biofilm precursor substances is caused by a high-nutrition environment is achieved through cooperation of whey protein hydrolysate, humic acid, chitosan and the like are solved, and the yield of the staphylococcus aureus bacteriophages is increased; the method solves the problems of bacteriophage infection efficiency reduction and amplification resistance, improves the bacteriophage titer, and shortens the fermentation period.

The invention discloses a preparation method and application of cinnamyl aldehyde nanoparticles, and the preparation method is characterized in that cinnamyl aldehyde is used as a functional component, zein is used as a carrier, sodium caseinate is used as a stabilizer, and pectin is used as a stabilizer and an adhesive to prepare the cinnamyl aldehyde nanoparticles. Experiments prove that the cinnamyl aldehyde nano-particles prepared by the invention can significantly improve the stability of cinnamyl aldehyde, enhance the antibacterial activity of cinnamyl aldehyde to staphylococcus aureus, and provide more choices for development and utilization of cinnamyl aldehyde.

The application discloses a metal-MOF nano-enzyme, a preparation method and application thereof, and aims to provide a metal-MOF nano-enzyme with hydrogen peroxide mimetic enzyme activity, which can significantly improve the antibacterial effect on E. coli and S. aureus and can be applied to E. coli and S. aureus bacteriostatic agents. The technical scheme is that metal salt solution is added into 2-methyl imidazole solution, and metal-MOF nano-enzyme is synthesized through ultrasonic water bath. The application belongs to the technical field of nano-mimetic enzymes and medical antibacterial technology.

The invention relates to the technical field of medicines, in particular to an extraction method and application of cordate houttuynia exosome-like nanoparticles. The method comprises the steps of pretreatment, impurity removal through differential centrifugation, fine filtration, ultra-speed centrifugation crude extraction, sucrose density gradient centrifugation purification, washing and the like. By optimizing key process parameters, the HELNs with the average particle size of 50-150nm and the typical double-layer membrane cup-shaped vesicle form can be stably obtained. Experiments prove that the HELNs prepared by the method have independent and direct anti-staphylococcus aureus activity. Based on the newly discovered biological activity, the invention provides new application of the HELNs in preparation of products (such as antibacterial drugs, dressings, coatings, lotions and the like) for inhibiting staphylococcus aureus and / or treating infection of staphylococcus aureus. The method is standard in operation and good in repeatability, and widens the application field of active ingredients of houttuynia cordata.

The invention belongs to the field of food processing, and particularly relates to a processing method of tea-flavored fresh braised chicken capable of prolonging the shelf life and an obtained product. According to the processing method provided by the invention, under the action of the tea polyphenol, on one hand, free radicals in a lipid oxidation chain reaction are competitively removed, grease rancidity and protein oxidative degradation are inhibited, and the rising rate of putrefaction indexes such as volatile basic nitrogen and a peroxide value is reduced; on the other hand, the cell membrane integrity of escherichia coli, staphylococcus aureus and other pathogenic bacteria and molds is destroyed, and metabolism and reproduction are inhibited. After the process is optimized, the shelf life of the Texas braised chicken under the normal-temperature vacuum packaging condition is prolonged to 15-20 days compared with that of the Texas braised chicken in the traditional process, artificially synthesized preservatives do not need to be added in the whole process, and the Texas braised chicken conforms to the natural and healthy food consumption trend.

The invention relates to a method including box boriding process to improve some mechanical properties such as hardness, corrosion resistance and wear and to provide 100% antibacterial properties to AISI 316L quality stainless steels, which are among the most widely used and known metals in terms of biocompatibility as implant materials. The invention relates to AISI 316L type biomaterial having 100% antibacterial properties according to antibacterial tests with Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 bacteria.

The application discloses a kind of biological coating with physical mechanical antibacterial and chemical antibacterial performance and preparation method thereof, it is related to biomaterial technical field.The biological coating of the application is composed of TC4 nanofiber network and nano copper deposited in nanofiber network, and the preparation method is: using polishing-washing-sweeping process pretreatment TC4 titanium alloy;It is placed in alkali solution and heated in water bath to activate, and the alloy after activation is repeated polishing, washing, sweeping step, to obtain the substrate to be sputtered;The substrate is magnetron sputtered, and nano copper is deposited on its surface;After depositing nano copper, the substrate is annealed in inert atmosphere and cooled, and the biological coating with physical mechanical antibacterial and chemical antibacterial performance is prepared.The synergistic effect of the biological coating composed of nano copper and nanofiber network can effectively kill staphylococcus aureus, escherichia coli and other pathogenic microorganisms, and can be applied to oral implant, metal implant biomaterial as functional coating.

The invention relates to the technical field of organic silicon materials, in particular to an antibacterial silica gel material and a preparation method thereof, and aims at solving the problems that in an existing antibacterial silica gel material, an antibacterial agent is poor in dispersity, the antibacterial durability is insufficient, and the mechanical property is affected. The antibacterial silica gel material is prepared from the following raw materials: an amino modified silicone rubber matrix, SiO2 coated-Cu coated CuO composite particles, a polyether modified polysiloxane dispersant and a vulcanizing agent, and the preparation method comprises the following steps: preparation of the amino modified silicone rubber matrix, preparation of the SiO2 coated-Cu coated CuO composite particles, segmented mixing of a composite rubber material, and vulcanization molding. Through raw material modification and process optimization, the antibacterial rate of the material to escherichia coli and staphylococcus aureus reaches 99% or above, the antibacterial performance is stable after recycling, the tensile strength is larger than or equal to 8.6 MPa, the elongation at break is larger than or equal to 520%, and the material can be widely applied to the fields of food contact appliances, daily protection articles and the like.

This invention relates to the application of a traditional Chinese medicine composition in the preparation of a drug for sinusitis. The composition is characterized by being prepared from ephedra (processed with honey), bitter almond, perilla seed, prepared pinellia, licorice, scutellaria, angelica, belamcanda, coltsfoot flower, mulberry bark, cicada slough, silkworm, and earthworm. The composition of this invention improves sinusitis symptoms, reduces inflammation levels, and regulates the pH of nasal secretions in a sinusitis model prepared by Staphylococcus aureus, suggesting that this traditional Chinese medicine composition has a good therapeutic effect on sinusitis.

The present disclosure relates to bacteriophages and compositions capable of infecting and killing Staphylococcus, and use of the same for treating Staphylococcus, e.g. Staphylococcus aureus, bacterial infections.

In certain embodiments, the present invention provides novel antibody purification methods and systems using a potentially simple and cost-efficient means. In some embodiments, customized Z-33 derived from Staphylococcus aureus Protein A is used to construct immuno-amphiphile molecules which can assemble into immunofibers in aqueous solution with bioactive epitopes on the surface and have IgG binding ability.

The invention discloses a herbal plant extraction moisturizing essence gel conditioning and soothing formula and a production process thereof, and relates to the technical field of cosmetics. According to the formula, water, glycerin and butanediol are used for constructing a triple moisturizing substrate, plant extraction conditioning components such as euglena parvata polysaccharide and myrothamnus flabellifolia extract are compounded, the honeysuckle flower extract and hexamidine bis (isethionate) salt are matched to achieve the mild antibacterial and anti-inflammatory effects, and all the components are combined according to the precise mass percent; according to the production process, the stability of the formula is guaranteed through the temperature control process of high-temperature dissolution, constant-temperature stirring and gradient cooling. The gel can achieve long-acting water locking for 48 hours, effectively inhibit breeding of harmful bacteria such as staphylococcus aureus and escherichia coli, relieve skin slight inflammation, moisten and nourish superficial layer meridians and collaterals of the skin surface and relieve limb acid precipitation caused by sedentariness or dryness, can improve the skin barrier toughness by 25% after being continuously used for 14 days, is clear and moist in texture and free of sticky feeling, does not need to build tolerance, and is free of side effects. The massager is suitable for multiple scenes such as light massage of sedentary people and is suitable for wide people.

The application discloses a D-type amino acid-based antibacterial peptide and application thereof, and has the amino acid sequence of w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized by Fmoc solid-phase chemical synthesis, has low synthesis difficulty, and has good in-vivo antibacterial activity. The antibacterial peptide has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, has low hemolytic toxicity, can reach more than half of the cell survival rate at a medium or low concentration, has almost no toxic effect on cells, has good stability, has strong operability, and is low in cost.

The application discloses a safe preservative-free sea dragon antibacterial peptide spray and a preparation method and application thereof. The spray contains sea dragon antibacterial peptide SsPle as an active ingredient and excipients. The amino acid sequence of the sea dragon antibacterial peptide SsPle is FFRSLWKGVKEGFRAGRSAYKA. The antibacterial peptide spray has the characteristics of safety, non-toxicity, non-inclusion of traditional preservatives, good stability, convenient use and the like. The antibacterial peptide has no acute oral toxicity, no mutagenicity and no chromosome damage risk, and has high biological safety. The spray is stable at 30 DEG C for 120 days, and still has a significant bacteriostatic effect on pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus (MRSA) and the like. The preparation process is simple, the spray can be filled by using a quantitative sprayer, and the spray is convenient for clinical treatment, wound care and daily disinfection application. The application solves the problem of irritation of traditional antibacterial peptide sprays, is suitable for sensitive people, and has a broad market prospect.

The invention discloses an inorganic plastic oxygen composite material and application thereof in a pet environment disinfection and deodorization system. The inorganic plastic oxygen composite material is prepared from the following components in parts by mass through a compounding process: an inorganic internal peroxide bridge composite compound; an inorganic mediated catalytic material; material balance and a stable system; according to the technical scheme, the defects of a traditional pet disinfection and deodorization product are thoroughly overcome, the product does not contain irritating chemical additives, the lavender essential oil is matched with a mild formula, residues and irritation are avoided during use, harm to pets and human bodies is avoided, and the safety requirement of pet scenes is met; the sterilization rate on escherichia coli and staphylococcus aureus during sterilization reaches 99.9% or above, the removal rate on peculiar smell gas such as ammonia gas and hydrogen sulfide exceeds 94%, the retention rate of effective oxygen is 95% or above, and unification of efficient sterilization, lasting deodorization and stable storage is achieved; meanwhile, the product is simple in preparation process, suitable for various scenes such as pet families and pet hospitals and convenient to operate.

The invention relates to the technical field of nano-silver composite coatings, in particular to a waterproof composite antibacterial coating containing nano-silver as well as a preparation method and application of the waterproof composite antibacterial coating. Oxalic acid (OA) and polyvinyl alcohol (PVA) are combined, nanocrystalline cellulose (NCC) is added to obtain a biodegradable film-forming material with excellent tensile strength, the film has good waterproof performance, the water solubility is greatly reduced, nano-silver is added to serve as an antibacterial agent, and the waterproof composite antibacterial coating containing nano-silver is obtained. The preparation method is easy to operate, mild in condition and free of harm to the environment and the human body. The preparation method provided by the invention has the advantages of high production efficiency, high product quality and the like, silver particles are uniformly distributed in the composite antibacterial coating and are relatively good in stability, and the composite antibacterial coating has a relatively good inhibition effect on escherichia coli (E.coli) and staphylococcus aureus (S.aureus) and has a very good application prospect.

The present application relates to the technical field of textiles, and particularly relates to a composite textile fabric with antibacterial and deodorant functions and a preparation method thereof.The preparation method of the fabric comprises the following steps: uniformly mixing cotton fibers, lyocell fibers, hemp fibers and bamboo fibers, and then processing the mixture into blended yarn; weaving the blended yarn into a grey cloth, and then sequentially performing the following steps: scouring, mercerizing, dyeing, setting, and preshrinking to obtain a premix cloth; adding the premix cloth into an antibacterial solution and stirring, then drying, and finally performing singeing and etching to obtain the composite textile fabric with antibacterial and deodorant functions; the antibacterial solution is a mixed solution of rosemary alcohol-soluble extract and chitosan.The present application realizes the synergistic effect of the natural antibacterial components of bamboo fibers and the rosemary alcohol-soluble extract-chitosan antibacterial solution, and the antibacterial rate of the fabric against bacteria such as escherichia coli and staphylococcus aureus is still kept above 95% after 50 times of soaping, which is significantly improved compared with the antibacterial performance of traditional single coating fabric.