255 results about "Staphylococcus aureus" patented technology

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

The invention discloses preparation and application of an anti-staphylococcus aureus IsdB antibody. The antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises HCDR1, HCDR2 and HCDR3 in the heavy chain variable region as shown in SEQ ID NO: 8; the light chain variable region comprises LCDR1, LCDR2 and LCDR3 in the light chain variable region as shown in SEQ ID NO: 16. The invention also provides nucleic acid for coding the antibody, a vector containing the nucleic acid and a cell containing the vector. In addition, experiments prove that the antibody can inhibit systemic infection of MRSA and resist invasion of MRSA to pneumonia, and has a good clinical application prospect.

The invention discloses an antibacterial peptide as well as a preparation method and application thereof. The antibacterial peptide has an amino acid sequence as shown in any one of SEQ ID NO: 1-11. The antibacterial peptide prepared by the scheme provided by the invention has very strong bacteriostasis and sterilization capabilities on methicillin-resistant staphylococcus aureus and multidrug-resistant escherichia coli, has application potential of becoming an antibacterial agent, a preservative or a disinfectant, and is not easy to generate drug resistance.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

Method and kit for topical treatment of a skin infection, in particular of superficial skin infections, caused by Staphylococcus aureus. The method comprises the steps of applying a photosensitizer to at least a part of the skin affected by the infection, and subjecting said part of the skin to first photons with a majority energy between 2.8 eV and 3.5 eV at a first energy level; and second photons with a majority energy between 1.24 eV and 1.65 eV at a second energy level, the ratio between the first and the second energy levels being in the range from about 10:1 to 2:1. By the method efficient treatment of Staphylococcus aureus infections can be achieved without the administration of topical antibiotics.

The invention belongs to the technical field of biomedicine and nano material electronics, and particularly relates to a low-voltage efficient sterilization electrode device based on local enhanced electric field treatment. According to the electrode device, vertically-oriented nanoneedles serve as a core functional layer, a nanoneedle array is arranged on a conductive substrate, the intensity of an electric field in a pinpoint area is amplified by multiple times through the lightning rod effect induced by the tips of the nanoneedles so as to achieve irreversible electroporation of a low-potential induced pathogen membrane, the length of the nanoneedles is 5-100 micrometers, and the length of the nanoneedles is 5-100 micrometers. The curvature radius of the needle tip is lt; and the sterilization device can realize nearly complete sterilization on escherichia coli and methicillin-resistant staphylococcus aureus within 5 minutes. The invention further provides a nanometer microneedle sterilization patch based on the local enhanced electric field, a self-power-supply or external-source-power-supply energy supply mode is adopted, and rapid sterilization of a wound can be achieved without medicine adding.

The invention discloses a novel bifidobacterium infantis strain and application of extracellular products of the novel bifidobacterium infantis strain in delaying skin aging and regulating skin micro-ecology, and bifidobacterium infantis is bifidobacterium infantis BSJM24L007 which is preserved in the China General Microbiological Culture Collection Center (CGMCC for short) and has the preservation number of CGMCC No.33258. The invention further discloses a preparation method of the novel bifidobacterium infantis strain and the application of the extracellular products of the novel bifidobacterium infantis strain. The bifidobacterium infantis extracellular product prepared by the invention has an ideal effect of delaying skin aging, enables the skin to be more compact and elastic, reduces the generation of wrinkles and maintains normal metabolism of the skin, has relatively high safety, can effectively reduce the skin infection problem caused by staphylococcus aureus and propionibacterium acnes, and has a good application prospect. Skin infection and inflammation are reduced, skin health is promoted, and skin micro-ecological balance is maintained. The requirements of a modern skin external preparation on functionality and safety are met.

The invention discloses a production method of a cool-feeling antibacterial PA66 chinlon filament, and belongs to the technical field of chemical fiber manufacturing. According to the method, PA66 slices are used as raw materials and matched with jade cool-feeling master batches and a septol antibacterial oil agent (the effective component is OPHB), and the PA66 polyamide filament with the cool-feeling antibacterial effect is prepared through the process steps of slice and master batch mixing, melt spinning, side blowing cooling, oiling, drafting and winding and the like. The cool-feeling antibacterial PA66 chinlon filament produced by the invention not only has excellent physical properties (the fineness is 40-50 dtex, the breaking strength is greater than or equal to 3.5 cN / dtex, and the elongation at break is 36-46%), but also has long-acting antibacterial property (the antibacterial rate to escherichia coli and staphylococcus aureus is greater than or equal to 99%, and the antibacterial rate after 50 times of washing is still greater than or equal to 98%) and stable cool feeling (the cool feeling value Q-max is greater than or equal to 0.2 W / (m.K)), and is safe, non-toxic, environment-friendly, simple in process and low in cost. The method is suitable for the field of close-fitting textiles such as warp knitting, weft knitting and hosiery knitting, and the industrial applicability is high.

The invention discloses a copper-based multifunctional close-fitting textile and a preparation method thereof. The copper-based multifunctional close-fitting textile comprises a copper conductive fiber layer which is blended and woven with any other fibers; the natural fiber layer is selected from any natural fiber and is blended with the copper conductive fiber layer, the product integrates antifungal, deodorizing and dustproof synergy and is selectively applied to various use fields, and the blended spinning of the copper conductive fiber layer and the natural fiber layer can make the antistatic performance be improved in a breakthrough manner; in a dry environment, due to static electricity generated by friction in natural fibers, copper ions can quickly disperse electrostatic charges and guide the electrostatic charges to the ground through an electron conduction mechanism, and static electricity accumulation is eliminated. Dust prevention is realized by electrostatic adsorption of particles. The copper ions can destroy bacterial cell membranes and inhibit reproduction of the bacterial cell membranes, and can treat common bacteria such as escherichia coli and staphylococcus aureus and fungi such as trichophyton rubrum and trichophyton asthenia. The photocatalytic material is used for photothermal catalytic decomposition of peculiar smell molecules.

The invention discloses a modified chitosan-based antibacterial material as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The ciprofloxacin grafted quaternized chitosan is successfully synthesized by taking the chitosan as a raw material, and the water solubility and the antibacterial ability of the chitosan are effectively improved. The antibacterial diameters of the ciprofloxacin grafted quaternized chitosan prepared by the preparation method disclosed by the invention on escherichia coli and staphylococcus aureus are respectively 35.34 to 39.06 mm and 40.45 to 44.51 mm, and the minimum MIC (Minimum Inhibitory Concentration) and MBC (Minimum Mass Chitosan) can reach 0.0005 mg / mL. In addition, the antibacterial material has an obvious inhibition effect on colletotrichum gloeosporioides and aspergillus niger, and the highest antifungal rates respectively reach 53.81 + / -2.17% and 54.88 + / -1.3%. Compared with the raw materials, the antibacterial ability is greatly improved, and the grafted and modified composite material is environment-friendly and can be used for antibacterial food packaging materials.

The invention belongs to the technical field of oral care, and discloses a rhodiola rosea-containing composite antibacterial oral bacteriostatic paste, which is prepared by compounding rhodiola rosea, tea polyphenol and other five-compound antibacterial components with sorbitol and other auxiliary materials according to an accurate proportion. According to the invention, a triple system of'instant sterilization-lasting bacteriostasis-ecological restoration 'is constructed, third-party detection shows that the inhibition rate on oral pathogens such as escherichia coli, staphylococcus aureus and the like reaches 99.9% or above, and multiple effects of resisting bacteria, diminishing inflammation, protecting gingiva, preventing caries and the like are achieved. The problems that an existing product is single in antibacterial effect, narrow in antibacterial spectrum and low in rhodiola rosea utilization rate are solved, no heavy metal is detected, stability is excellent, irritation is low, and the rhodiola rosea antibacterial agent is suitable for long-term use and has remarkable innovativeness and industrial value.

We provide new monoclonal antibody inhibitors of coagulases, staphylocoagulase (“SC”) and von Willebrand factor-binding protein (“vWbp”) for treatment of S. aureus. The monoclonal antibodies are useful in targeting the SC N-terminus of SC and vWbp (respectively) and inhibiting prothrombin activation. The monoclonal antibodies are able to bind to and interfere with, modulate, and / or inhibit the binding interactions between the coagulase protein and its ligan protein prothrombin the activation of prothrombin.

This invention discloses a method for preparing a chitosan antibacterial film. First, berberine aqueous solution and tannic acid aqueous solution are prepared separately. Then, the berberine aqueous solution is added dropwise to the tannic acid aqueous solution to form a mixed solution. The mixed solution is stirred and heated to obtain a suspension. The suspension is freeze-dried to obtain berberine-tannic acid nanoparticles. Next, a chitosan acetic acid aqueous solution is mixed with the berberine-tannic acid nanoparticles, and a film is formed to obtain a chitosan antibacterial film loaded with berberine-tannic acid nanoparticles. The prepared chitosan antibacterial film has low water content and swelling degree, strong ultraviolet shielding ability, high transparency, and strong inhibitory activity against Staphylococcus aureus and Salmonella typhimurium, which can better meet the needs of food preservation.

The application belongs to the technical field of microorganisms and particularly relates to a Bifidobacterium adolescentis beneficial to skin, a derivative of the Bifidobacterium adolescentis and application of the Bifidobacterium adolescentis and the derivative. The derivative of the Bifidobacterium adolescentis has significant antioxidant and anti-inflammatory effects, can inhibit pathogenic bacteria of acne and other common skin infections such as Propionibacterium acnes, Staphylococcus aureus and Malassezia furfur, can eliminate pathogenic bacteria, can inhibit skin lipid accumulation, can avoid follicle blockage, and can improve skin problems in multiple directions, especially acne skin problems.

The application discloses a silver / schiff base antibacterial material, a preparation method and application, and relates to the field of antibacterial materials.The silver / schiff base antibacterial material, the preparation method and the application are characterized in that, based on the large electron cloud density of the carbon-nitrogen double bond of the schiff base, silver ions in a silver nitrate solution are uniformly adsorbed on a schiff base base through the electrostatic adsorption principle; under the reduction of a reducing agent, the silver ions are reduced to nanosilver ions, and a silver / schiff base antibacterial material is obtained, in which nanosilver ions in the form of nanospheres are uniformly dispersed on the schiff base in the form of a thin sheet layer, thereby greatly improving the dispersibility of the nanosilver ions.The preparation method is simple, low in cost, and capable of batch production, and has no cytotoxicity, and has high antibacterial effects on streptococcus, pseudomonas aeruginosa and staphylococcus aureus, and has a good application prospect.

The invention discloses an antibacterial hydrogel dressing taking spunlace non-woven fabric as a carrier and a preparation method of the antibacterial hydrogel dressing, and relates to the technical field of biomedical polymers. The antibacterial hydrogel dressing is composed of a spunlace non-woven fabric carrier layer and an antibacterial hydrogel functional layer loaded on the surface and in pores of the spunlace non-woven fabric carrier layer, and the preparation method comprises the following steps: sterilizing spunlace non-woven fabric to obtain a spunlace non-woven fabric carrier; adding the antibacterial component into the aqueous solution of the hydrophilic polymer base material to form a hydrogel precursor solution; and immersing the spunlace non-woven fabric carrier into the hydrogel precursor solution, and curing to obtain the antibacterial hydrogel dressing. The antibacterial hydrogel dressing prepared by the preparation method disclosed by the invention has the flexible breathable characteristic of spunlace non-woven fabric and the high swelling and antibacterial functions of hydrogel, has an excellent bactericidal effect on escherichia coli and staphylococcus aureus, can effectively promote healing of infected wounds, is suitable for clinical nursing of burn and traumatic skin injuries, and has wide application prospects. Wide clinical application prospects are realized.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

The invention provides a breathable antibacterial woven special fabric producing and processing device which comprises an antibacterial processing main body cabin, a moisture-proof base, a feeding and discharging slope plate, a folding sliding door assembly, an intelligent control cabinet assembly, a ventilation motor, a ventilation pipeline, a fabric tightness adjusting rod assembly and a fixed stretching rod. The antibacterial processing main body cabin body is in bolted connection with the upper part of the moisture-proof base; the feeding and discharging slope plate is connected with the front portion of the damp-proof base through screws and is close to the bacteriostasis processing main body cabin body. Through the DBD plasma array, the porous aerosol generator and the full-spectrum LED array, good antibacterial performance can be achieved, especially the killing rate of escherichia coli / staphylococcus aureus is increased, and the antibacterial rate is still kept above the national standard after multiple times of washing; the whole antibacterial treatment process is physical, chemical raw materials are avoided, the safety is improved, no toxic substance is generated, and the breaking strength loss of the textile is low.

Described herein is a synergistic combination comprising a quorum-sensing inhibitor and / or postbiotic metabolite and an antibiotic. Typically, the postbiotic metabolite comprises at least one peptide. Related compositions, uses, and methods are also described, including methods for resensitizing resistant bacteria to an antibiotic, and methods of treating antibiotic-resistant infections, such as methicillin-resistant Staphylococcus aureus (MRSA).

The invention discloses a method for rapidly and efficiently preparing a series of novel ester group facaplysin derivatives by combining EDCI and HOBT to react with carboxyl facaplysin and directly carrying out ester exchange reaction on an active ester intermediate and alcohol without separation. The invention also discloses application of the derivatives in resisting methicillin-resistant staphylococcus aureus and escherichia coli. The preparation method comprises the following steps that EDCI and HOBT are combined to activate carboxyl facaplysin for reaction, an activated ester intermediate is generated, the activated ester intermediate and alcohol are directly subjected to esterification reaction without separation, and a series of ester-based facaplysin derivatives are obtained. According to the preparation method, ester-based Fascaplysin derivatives with different structures can be rapidly and efficiently constructed, and compared with a traditional method that ester-based carboline derivatives are firstly synthesized and then high-temperature intramolecular cyclization is performed, the time and the raw material cost are greatly saved, the synthesis efficiency is improved, the reaction process is observable and controllable, and the yield is stable. And the synthesized ester group facaplysin derivative has excellent antibacterial activity on methicillin-resistant staphylococcus aureus and escherichia coli.

In some aspects, disclosed herein are compounds of the formula: wherein the variables are defined herein. Also provided are pharmaceutical compositions thereof. In some aspects, the compounds and compositions provided herein may be used to modulate the activity of Staphylococcus aureus. Also provided are methods of administering compounds and composition provided herein to a patient in need thereof, for example, for the treatment or prevention of diseases or disorders.

This invention discloses a bicyclic [2.1.1]hexene compound and its preparation and application. The invention uses bicyclic [1.1.0]butanone and phosphate ester as starting materials, and potassium tert-butoxide tetrahydrofuran solution as base. The reaction is carried out under reflux at 80°C to obtain the bicyclic [2.1.1]hexene compound. The prepared compound has certain transpeptidase inhibitory activity and can effectively inhibit the infection and spread of Staphylococcus aureus.

Methods of treating preventing and / or substantially reducing a Pseudomonas aeruginosa (PA) infection and / or a Staphylococcus aureus (SA) infection by administration of a bivalent protein subunit-based vaccine are described. The vaccine can protect subjects with a prior history of exposure to SA from a new exposure to PA or SA, including methicillin resistant SA (MRSA), as well as being able to protecting with no prior exposure to a SA infection. Vaccine formulations are also described.

This invention relates to the field of plant extract application technology, particularly to banana fruit extract and its applications. This invention reveals that banana fruit extract exhibits excellent performance in relieving inflammation, anti-oxidation, scavenging free radicals, delaying cell aging, inhibiting viruses and bacteria (inactivating HPV, Staphylococcus aureus, Candida albicans, Escherichia coli, and Propionibacterium acnes), reducing infection, promoting cell regeneration and repair, promoting collagen synthesis, enhancing elasticity and firmness, and acting as a plant-derived immunomodulator. Furthermore, it serves as a natural, intelligent transdermal carrier. Safe and non-irritating, this extraction method has shown excellent application value in high-value plants such as saffron, rose, Dendrobium officinale, ginseng, and Ganoderma lucidum. It provides a comprehensive solution for skin from defense to regeneration, offering a regulatory-compliant natural solution for functional skincare.

In certain embodiments, the present invention provides novel antibody purification methods and systems using a potentially simple and cost-efficient means. In some embodiments, customized Z-33 derived from Staphylococcus aureus Protein A is used to construct immuno-amphiphile molecules which can assemble into immunofibers in aqueous solution with bioactive epitopes on the surface and have IgG binding ability.

The invention relates to the technical field of microorganisms, in particular to a culture medium for staphylococcus aureus phage fermentation and a fermentation method. According to the culture medium for staphylococcus aureus bacteriophage fermentation, the problems that in large-scale culture of staphylococcus aureus bacteriophages, excessive accumulation of host bacteria biofilm precursor substances is caused by a high-nutrition environment is achieved through cooperation of whey protein hydrolysate, humic acid, chitosan and the like are solved, and the yield of the staphylococcus aureus bacteriophages is increased; the method solves the problems of bacteriophage infection efficiency reduction and amplification resistance, improves the bacteriophage titer, and shortens the fermentation period.

The invention discloses a preparation method and application of cinnamyl aldehyde nanoparticles, and the preparation method is characterized in that cinnamyl aldehyde is used as a functional component, zein is used as a carrier, sodium caseinate is used as a stabilizer, and pectin is used as a stabilizer and an adhesive to prepare the cinnamyl aldehyde nanoparticles. Experiments prove that the cinnamyl aldehyde nano-particles prepared by the invention can significantly improve the stability of cinnamyl aldehyde, enhance the antibacterial activity of cinnamyl aldehyde to staphylococcus aureus, and provide more choices for development and utilization of cinnamyl aldehyde.

The present application relates to methods of treating or preventing infection-related immune conditions using compositions comprising IgM. In particular, the present application provides a method for treating immune complications resulting from infection in a patient in need thereof, characterized in that the method comprises administering a composition comprising IgM. In some embodiments, the treatment of immune complications is carried out by immunomodulation. Also provided is a method for immunomodulating an infection in a patient in need thereof, characterized in that the method comprises administering a composition comprising IgM. The present application also provides a composition comprising IgM for use in the treatment of sepsis and a composition comprising IgM for use in the treatment of immune complications resulting from infection. In some embodiments, the above-mentioned infection or sepsis results from E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus pneumoniae, Clostridium difficile, Clostridium botulinum, or a combination thereof.

The application discloses a metal-MOF nano-enzyme, a preparation method and application thereof, and aims to provide a metal-MOF nano-enzyme with hydrogen peroxide mimetic enzyme activity, which can significantly improve the antibacterial effect on E. coli and S. aureus and can be applied to E. coli and S. aureus bacteriostatic agents. The technical scheme is that metal salt solution is added into 2-methyl imidazole solution, and metal-MOF nano-enzyme is synthesized through ultrasonic water bath. The application belongs to the technical field of nano-mimetic enzymes and medical antibacterial technology.