3354 results about "Staphylococcus aureus" patented technology

The present invention relates to characterizing changes in mammalian bacterial gastrointestinal, cutaneous and nasal microbiota associated with antibiotic treatment and various disease conditions (such as asthma, allergy, obesity, metabolic syndrome, gastrointestinal reflux disease (GERD), eosinophilic esophagitis, gastro-esophageal junction adenocarcinomas (GEJAC), infections due to bacteria that are resistant to antibiotics, including Methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile, vancomycin-resistant enterococci, etc.) and related diagnostic and therapeutic methods. Therapeutic methods of the invention involve the use of live bacterial inoculants that are capable of restoring healthy mammalian bacterial gastrointestinal, skin, and nasal microbiota.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention relates to anti-bacterial water-based paint and a preparation method thereof. The paint comprises the following components in parts by weight: 0.2-11 parts of anti-bacterial agent, 8-33 parts of nano material, 23-64 parts of water-based resin dispersoid and 0.75-18 parts of adhesive resin or plasticizer. The preparation method comprises the following steps: firstly preparing a nano silver anti-bacterial agent; mixing deionized water, the anti-bacterial agent, a wetting agent, a dispersing agent and a defoaming agent and uniformly mixing, adding the nano material, uniformly dispersing to obtain the water-based dispersoid; adding the obtained water-based dispersoid to the mixed emulsion or water-based resin dispersoid, then adding the adhesive resin or plasticizer and various conventional assistants, stirring and dispersing evenly; adding pigments or colorant; and supplementing water to obtain the anti-bacterial water-based paint. The long-acting broad-spectrum antibacterial water-based paint has high fungicidal efficiency (more than 99%) on escherichia coli, staphylococcus aureus, black varietas of bacillus subtilis and the like and can reduce the high concentrate of organic matters of formaldehyde to the range of specified concentration index.

The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

The invention relates to an antibacterial finishing method for textile containing cellulose. The method uses natural polymer chitosan and sodium alginate as wall materials; traditional Chinese medicine extracts are used as a core material and are subjected to encapsulation by a micro capsule technology; then an afterfinishing method is employed to treat the microcapsule to the partially carboxymethylated textile containing cellulose, so as to endow the textile with antibacterial performance and good wash fastness through electrostatic attraction between the carboxyl on cellulose fiber and chitosan, as well as the crosslinking effect of the cross-linking agent. The raw materials employed by the invention are green, environment-friendly, safe and high-efficiency, and the obtained textile has good antibacterial effect on common staphylococcus aureus, Eschierichia coli and bacillus subtilis.

The invention relates to an antibacterial mildewproof polylactic acid composition and a preparation method thereof. The composition comprises the following mixed components: polylactic acid, an antibacterial mildewproof agent, and a dispersant; wherein on a basis of 100 parts by weight of polylactic acid, the composition comprises 0.3-2 parts by weight of antibacterial mildewproof agents, and 0-0.5 parts by weight of dispersants; the antibacterial mildewproof agent is a polyguanidine pyrithione antibacterial mildewproof agent which is obtained by mixing an aqueous solution of water-soluble polyguanidine inorganic acid salts or organic acid salts and an aqueous solution of sodium pyrithione; the molar ratio of the water-soluble polyguanidine inorganic acid salts or organic acid salts and sodium pyrithione is 1:(0.1-5). The polylactic acid composition of the invention has good antibacterial and mildewproof effect. The antibacterial effect on staphylococcus aureus and escherichia coli is up to 99.9%; the mildewproof grade for mold such as aspergillus niger, aspergillus terreus, paecilomyces varioti bainier, and the like reaches 0 grade; the composition has good water resistant stability, and has wide application prospects.

A combined nano antiseptic material and its preparation method and products are disclosed. The advantages of the invention lie in 1.combining different antiseptic material and preparing combined nano antiseptic material, taking advantage of synergism and coupling effect of between nano particle, promoting the antiseptic ability 2.The combined antiseptic material has wide spectrum antiseptic function and killing effect to staphylococcus aureus etc. 3.The method can be used in producing sorts of bacteria and virus killing antiseptic material ,such as antiseptic fabric, latex etc.

Nucleic acid arrays and methods of using the same for concurrent or discriminable detection of different strains of a non-viral species. In many embodiments, the nucleic acid arrays of the present invention include probes that are specific to different respective strains of a non-viral species. In many other embodiments, the nucleic acid arrays of the present invention include probes that are common to two or more different strains of the non-viral species. In one embodiment, the non-viral species is Staphylococcus aureus, and the different Staphylococcus aureus strains include COL, N315, Mu50, EMRSA-16, MSSA-476, and 8325 strains. In another embodiment, a nucleic acid array of the present invention includes polynucleotide probes capable of hybridizing under stringent or nucleic acid array hybridization conditions to respective sequences selected from SEQ ID NOs: 1 to 7,852, or the complements thereof.

The invention provides bacillus subtilis K018 which is strong in stress resistance, excellent in probiotics function and safe and reliable, a biological feed additive containing the bacillus subtilis K018 and use thereof. The bacillus subtilis K018 provided by the invention can resist artificial gastric acid, cholate, artificial intestinal juice and high temperature, has stronger inhibiting effects to pathogenic staphylococcus aureus, escherichia coli, salmonella enteritidis and the like in the intestinal tract, is stronger in amylase and cellulase generating capacity, and can degrade starch and cellulose.

Aqueous dilutions of acidic ternary compositions having unusually synergistic antimicrobial activity against Staphylococcus aureus and Salmonella choleraesuis microorganisms are disclosed. The compositions encompass ternary component mixtures of an alpha-hydroxycarboxylic acid, a monohydric water soluble alkanol and an anionic tenside having a Ternary Ratio residing within a defined phase region wherein synergistic antimicrobial activity is observed; whereas mixtures outside of the defined phase region and those lacking any one of the components do not exhibit synergistic activity. Compositions, methods and articles employing the inventive ternary synergistic compositions, and their utility in sanitizing and disinfecting hard surfaces are described.

The invention relates to the detection field of microorganism foodborne pathogens and discloses a method for rapidly detecting and screening staphylococcus aureus, which adopts bio-functionalized superparamagnetic nano particles and immunized quantum dots to immunologically recognize target microorganisms to realize to specificity detection on the foodborne pathogens (staphylococcus aureus). According to the method, target bacteria can be rapidly separated from various samples, and enrichment is also performed efficiently; and besides, bacteria separated by the enrichment can be marked rapidly by fluorescence, so that the bacteria can be qualitatively identified by a fluorescence microscope and can be quantitatively detected by a fluorescence photometer. The method is convenient and simple to operate, and has high reliability and a low requirement on corollary equipment.

Disclosed herein are isolated and purified Staphylococcus aureus bi-component leukocidin, referred to herein as LukAB, and its components LukA and LukB, antibodies specific to LukA, antibodies specific to LukB, therapeutic compositions containing LukA and/or LukB, or anti-LukA and/or anti-LukB antibodies, uses of the compositions to treat acute inflammatory conditions or S. aureus infection, methods for identifying inhibitors of LukAB-mediated cytotoxicity of human phagocytes, and methods for using LukAB as a marker to predict severity of S. aureus infection.

The invention discloses lactobacillus plantarum GLM101 and an application thereof in preparing a feed additive. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number CGMCC No. 11156. The lactobacillus plantarum GLM101 has very strong inhibiting ability for escherichia coli, staphylococcus aureus, salmonella typhi, vibrio vulnificus and aeromonas hydrophila, and also has excellent acid-resisting and cholate-resisting abilities. The lactobacillus plantarum GLM101 can be used for adjusting the microecological balance inside the intestines of animals, has the action of enhancing the nonspecific immunity function to prevent diseases, and also can provide trophic factors, promote digestive absorption of nutrients, promote growth of animals, and improve the feed conversion ratio.

Described are synergistic antimicrobial-fragrance compositions including broad spectrum antimicrobial compositions containing salicylaldehyde and at least one organoleptically-compatible antimicrobial synergism cofactor substance. The weight ratio range of salicylaldehyde:synergism cofactors substance is from 1:10 up to 10:1. The cofactor substance is such that the degree of synergism of the resultant mixture is defined according to the IFF Antimicrobial Synergism Test wherein the difference between the actual and expected antimicrobial values of the mixture is greater than or equal to a multiple of (i) 0.05 and (ii) the expected antimicrobial value of the mixture. Cofactor substances include phenolics such as cresol, caravacrol and thymol; ethyl vanillin; benzyl alcohol; indol; beta-orcinol; and terpinenol-4. Microorganisms against which the synergistic compositions are effective include:Escherichia coli;Enterococcus hirae;Pseudomonas aeruginosa;Staphylococcus aureus; andSaccharomyces cerevisae.The compositions have application in all-purpose cleaning compositions, gel-type toilet rim articles, liquid-type toilet rim articles, personal shower cleaning compositions, and body and hair care products including shower gel compositions, shampoo compositions and foam bath compositions.

The invention discloses a preparation method of antibacterial silver/chitosan nano fibrous membranes, pertaining to the preparative technologies of nano composite fibrous membranes. The process of the method includes that a chitosan hexanoic acid solution, a silver nitrate aqoeous solution, a sodium borohydride water solution and an ethylene epoxide hexanoic acid solution are prepared and mixed to form compounded latex according to the volume ratios of the chitosan hexanoic acid solution and the silver nitrate aqoeous solution as well as an NaBH4 aqueous solution. The compounded latex and the ethylene epoxide hexanoic acid solution are mixed according to the volume ration to prepare a spinning solution, then the spinning solution is added into an injector in an electrostatic spinning device and electrostatic spinning is carried out to form the fibrous membrane. Crosslinking treatment is carried out to the fibrous membrane to obtain the antibacterial silver/chitosan nano fibrous membrane. The antibacterial silver/chitosan nano fibrous membrane of the invention has the advantages that the preparation process is simple; the prepared membrane material has broad-spectrum bactericidal property and comparatively high fatality rate to Bacillus coli, Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa for 24 hours.

Compounds having unique antiviral and antibacterial properties are prepared from medicinal mushroom mycelium, extracts and derivatives. The compositions are derived from Fomitopsis, Piptoporus, Ganoderma, Inonotus, Trametes, Pleurotus, and blends of medicinal mushroom species and are useful in preventing and treating viruses including Poxyiridae and Orthopox viruses, flu viruses including bird flu (H5N1), SARS and Hepatitis C(HCV), as well as infections from Mycobacterium tuberculosis, Staphylococcus aureus and Escherichia coli.

The invention belongs to the field of nanometer materials and food packaging materials, and discloses a nanofiber composite membrane containing plant source antibacterial agents, a preparation method and application of the nanofiber composite membrane. The preparation method of the nanofiber composite membrane includes a first step of preparing 6%-10%, by mass, of polyving akohol solution, a second step of cooling the obtained polyving akohol solution down to 30-50 DEG C, adding 1%-3% of beta-cyclodextrin, carrying out magnetic stirring for 0.5-1 hour, and then cooling the mixtures down to indoor temperatures, a third step of adding 2%-8% of cinnamon essential oil to the solution of the second step, carrying out magnetic stirring for 1-2 hours at 25-40 DEG C, and obtaining spinning solution, and a fourth step of carrying out electrostatic spinning on the obtained spinning solution, carrying out vacuum drying, and obtaining the nanofiber composite membrane containing the plant source antibacterial agents. The sterilizing rate of the nanofiber composite membrane for staphylococcus aureus and escherichia coli is larger than or equal to 90%, and the nanofiber composite membrane has good tenacity, biocompatibility and degradability, and has wide application prospect in the field of the food packaging materials.

The invention relates to an andrographolide derivative, which has the structure as shown in a general formula I: wherein, R1, R2 and R3 are the same or different hydrogen, substituted or non-substituted organic acid radical, inorganic acid radical, alkyl, aryl or heteroaryl, while at least one of R1, R2 and R3 is R-lipoic acid or S-lipoic acid or the mixture of R-lipoic acid and S-lipoic acid or the corresponding dihydrolipoic acid or acetylcysteine radical of R-lipoic acid or S-lipoic acid; the derivative has good anti-tumor effect, and the derivative can cause apoptosis of tumor cells, directly eliminate gram positive bacteria, staphylococcus aureus and sensitivities MRSA5676 and MRSA5677, inhibit the QS system of gram negative bacteria and pseudomonas aeruginosa and inhibit and damage the formation of the bio-film of pseudomonas aeruginosa; the product has prominent hypoplycemic effect and is suitable for the preparation of the medicines that can cure cancers, inflammations, diabetes mellitus and bacterial and viral infection.

The invention relates to nano-silver solution with the nano-silver concentration between 250 and 3,200ppm and the average sliver grain diameter between 2 and 12nm. The nano-silver solution also comprises medicine accessory level dispersing agents and medicine level reducing agents. The preparation method mainly comprises the following steps of: dissolving the medicine accessory level dispersing agents into deionized water to prepare dispersing agent solution; adding and dissolving silver nitrate to obtain solution I; taking and adding the medicine accessory level reducing agent solution into the solution I; mixing the medicine accessory level reducing agent solution with the solution I to obtain solution II; placing the solution II under an ultraviolet lamp to be irradiated; and stirring the solution II for reaction to obtain the nano-silver solution. The nano-silver solution has stronger antibiosis performance. When the nano-silver solution is diluted to 10ppm to detect and evaluate the antibiosis effect on staphylococcus aureus, colibacillus and pseudomonas aeruginosa, the antibiosis efficiency reaches higher than 99.9 percent in two minutes. The preparation method has simple process, can avoid the use of various strong acid, strong base and strong reducing agents and has good medical safety.

The invention discloses a method for preparing a nanofibre membrane, belonging to a technique for preparing a nanofibre compound membrane. The method comprises the following steps: preparing curcuminethanol solution and chitosan acetum, mixing the curcumin ethanol solution and the chitosan acetum by the volume ratio thereof to prepare spinning solution which is injected into an injector of an electrostatic spinning device for electrostatic spinning to form a compound nanofibre membrane and to obtain a curcumin/chitosan nanofibre membrane of antibacterial property, wherein the diameter of thecurcumin/chitosan nanofibre membrane is 200-400 nm. The invention has the advantages that the preparation process is simple, the prepared membrane material heals wound, has broad spectrum bactericidalproperty and has higher bacterial inhibition rate to colibacillus and staphylococcus aureus for 24h.