Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and microbial strains, applied in the direction of application, bacteria, and microorganisms, can solve the problems of antibiotic resistance, excessive antibiotic residues in animal food, etc., and achieve the goal of improving production performance, improving health, and saving costs Effect

Active Publication Date: 2015-12-09
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its abuse in animal feed has resulted in excessive antibiotic residues in animal food, putting animals and humans at risk of developing resistance to antibiotics

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Isolation, screening, identification and preservation of Lactobacillus plantarum GLM101

[0015] 1.1 Isolation of strains

[0016] Take feces samples from healthy piglets, dilute them by 10, 100, and 1000 times with sterile water respectively, take 200 μL and spread them on MRS solid medium, incubate at 37°C for 48 hours, pick colonies, streak MRS medium, and purify 4 times Afterwards, a single colony was picked and cultured in MRS broth to the logarithmic phase, added with a final concentration of 20% glycerol, and stored at -80°C for future use. After Gram staining, catalase test, nitrate reduction test and 16SrDNA sequencing.

[0017] 1.2 Identification of strain morphological characteristics

[0018] Take a single colony of the purified strain (named GLM101), transfer it to MRS solid medium (agar), and culture it in a constant temperature incubator at 37°C for 24h, 36h, and 48h, and observe the size, color, edge, and convexity of the colony, respectively...

Embodiment 2

[0078] The preparation method of embodiment 2 bacterial strain GLM101 solid fermentation product

[0079] 1) Preparation of strains and their seed solution

[0080] The lactic acid bacteria strain GLM101 was activated on the MRS agar plate, and a single colony was picked and inoculated in the MRS liquid test tube. After culturing at 37°C for 24 hours, it was transferred to the MRS liquid medium with 2% inoculum, and cultured for 24 hours as the seed liquid.

[0081] The main components of MRS medium are peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, Tween 801.0mL, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, sulfuric acid Magnesium 0.58g, manganese sulfate 0.25g, distilled water 1000mL, pH6.2~6.6. The optimum pH is 6.0, the optimum temperature is 37°C, facultative anaerobic.

[0082] 2) Vaccination

[0083] The prepared seed solution was inoculated into the configured high-density solid medium (containing...

Embodiment 3

[0087] Example 3 Effect of solid fermentation product of bacterial strain GLM101 on production performance of nursery pigs

[0088] 3.1 Experimental animals and grouping

[0089] A total of 216 weaned piglets at the age of 32 days were selected as the experimental subjects, including 108 piglets in the experimental group and 108 piglets in the control group, and were fed to 77 days old. The experiment started from March 4, 2013 to April 18, 2013, and lasted for 45 days.

[0090] 3.2 Experimental design

[0091] Control group 1: the basal diet was not added with solid fermentation products of the strain GLM101;

[0092] Control group 2: Add 0.1% of a similar product in the market to the basic diet;

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Abstract

The invention discloses lactobacillus plantarum GLM101 and an application thereof in preparing a feed additive. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number CGMCC No. 11156. The lactobacillus plantarum GLM101 has very strong inhibiting ability for escherichia coli, staphylococcus aureus, salmonella typhi, vibrio vulnificus and aeromonas hydrophila, and also has excellent acid-resisting and cholate-resisting abilities. The lactobacillus plantarum GLM101 can be used for adjusting the microecological balance inside the intestines of animals, has the action of enhancing the nonspecific immunity function to prevent diseases, and also can provide trophic factors, promote digestive absorption of nutrients, promote growth of animals, and improve the feed conversion ratio.

Description

technical field [0001] The invention relates to a new bacterial strain and its application, in particular to the isolation and identification of a plant lactobacillus GLM101, and its application in bacteriostats and feed additives. Background technique [0002] In the field of farming technology, the use of antibiotics can effectively prevent animal diseases, reduce animal mortality, and reduce the risk of meat contamination. However, its abuse in animal feed has resulted in excessive antibiotic residues in animal food, putting animals and humans at risk of developing resistance to antibiotics. Since 2006, the European Union will completely prohibit food animals from eating antibiotic growth-promoting feed additives. Moreover, the risks caused by antibiotics to the health of animals and humans have also attracted the attention of my country's agricultural sector. Strengthening the management of rational use of antibiotics in the breeding process and excessive antibiotic res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P1/00A23K1/16C12R1/25
Inventor 张宏刚李莉
Owner GUANGZHOU GLAM BIOTECH
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