42 results about "Animal mortality" patented technology

The invention relates to a pediococcus pentosaceus G11 strain as well as screening and applications of the pediococcus pentosaceus G11 strain. The pediococcus pentosaceus G11 strain is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC NO:2016248. The pediococcus pentosaceus G11 strain has tolerance to compound sulfamethoxazole and ceftazidime, has antibacterial activity for vibrio parahaemolyticus, aeromonas hydrophila, vibrio alginolyticus, staphylococcus aureus and beta streptococcus, has certain stress resistance and enzyme producing ability, is free of hemocyte solubility, and has no drug tolerance for most antibiotics; the dipping bath experiment proves that the pediococcus pentosaceus G11 strain is harmless to the host, can improve the immunity of the body, has wide application prospects, can be applied to probiotic preparations, and also can be prepared into bacterial powder which is then added into a feed as a feed additive, further, the growth of pathogenic bacteria in the bodies of the animals is inhibited, so that the death rate of animals is reduced, and the pediococcus pentosaceus G11 strain is a probiotic brain worthy of development.

A process for preparing VHB mouse model with high expression of HBsAg includes such steps as linearizing carrier pcDNA3 by EcoRV, dephosphorylating by alkaline phosphatase of ox's small intestine, recombining the clonal carrier p3.6II, enzyme severing by PvuII to obtain 1.1-time HBV gene fragment, linking it with linear pcDNA3, transforming colibacillus competent cell DH5 alpha, screening resistance, enzyme serving, sequencing to verify the recombinant plasmid, injecting it in the mouse body via tail vein, and verifying the model.

The invention relates to a rat transplantable lung cancer model establishing method including the following steps: Walker-256 cell ascitic fluid is prepared; Walker-256 cell is inoculated into SD rat (Sprague-Dawley rat) lung; after anesthesia of a SD rat, left part of thoracic cavity of the SD rat is disinfected by use of povidone-iodine, Walker-256 cell suspension and Matrigel in same volume are mixed evenly on the ice to obtain a mixed solution, the mixed solution is slowly injected between 5th rib and 6th rib at left lung into the left lung by use of an injector, the puncture depth is 0.6-0.8 cm, after the injection, a needle is stopped for 10 s, and then is pulled out slowly, and after the rat is grown for 14 days, a transplantable lung cancer model is established. According to the method, the damage to the rat is small, the Walker-256 cell can be directly transplanted into the left lung only by use of a generally anaesthetized rat, formation of tumor lesion part is clear, the tumor lesion is single and controllable in size, and the method also has the advantages of simple preparation, high tumor formation rate, low animal mortality and the like.

A method for creating the animal model of the thrombosis in deep vein of limb without wound includes such steps as reforming the intramedullary nail driving-pulling tool to obtain a quantitative beater, and simulating the surgical wounding procedure to generate different wounds on lower limb of rabbit, resulting in thrombosis in deep vein. Its advantages are simple method and low dead rate.

The invention provides bacillus subtilis DCU. The bacillus subtilis DCU has a preservation number of CCTCC NO. 2014118, has good pathogenic microorganism resistance activity, can well inhibit pathogenic bacterium growth and breeding, can improve human immunity, has a wide application prospect, can be used in a probiotics preparation, can also be processed to form bacterial powder and can also be used as a feed additive for inhibiting pathogenic bacterium growth in animals and reducing animal mortality.

The invention provides a bacillus pumilus BP with the collection number of CCTCC NO:M2014117. The bacillus pumilus is good in anti-pathogenic activity, capable of greatly inhibiting the growth and propagation of pathogenic bacteria, capable of improving the body immunity, wide in application prospect, applicable to a probiotic preparation, and also capable of being prepared into bacterial powder and added in a feed as a feed additive for inhibiting the growth of pathogenic bacteria in the bodies of animals and reducing the death rates of animals.

The invention provides a method for establishing a coronary artery microvascular spasm pig-model, and belongs to the technical field of medical models. The method comprises that after bilateral femoral arteries of a test pig are free, distal ends of the femoral arteries are ligatured by wires, a left-side sheathing canal is fed into a pigtail catheter, blood flow dynamics indexes are determined, in a right-side sheathing canal, a coronary artery radiography process is carried out so that the middle of an anterior descending branch is displayed, and a neuropeptide Y is injected into the coronary artery so that the coronary artery microvascular spasm pig-model is obtained. The coronary artery microvascular spasm pig-model has good singularity, well fits to the clinical disease fact, satisfies clinical psychological stress characteristics, can be operated simply, is scientific and reasonable, has short modeling time, small damage and low animal mortality, realizes coronary artery microvascular evaluation on the carrier, realizes qualitative and quantitative evaluation of microvascular spasm state and degree, realizes real-time observation, provides a novel research means for basic clinical research and is suitable for popularization application.

The invention belongs to the technical field of medicine, and discloses a dual-heart disease animal model construction method, time and feed formula based on the syndrome of intermingled phlegm and blood stasis. A rat high-fat feed formula comprises 2% of cholesterol, 5% of lard, 0.2% of sodium cholate, 20% of cane sugar and 72.8% of basal feed. The method comprises the following steps that: feeding with the rat high-fat feed for 8 weeks is performed; on the first day of modelling, rats receive various unpredictable stresses every day, including nine kinds of stimuli such as fasting for 24 hours, depriving for 24 hours, swimming in ice water, binding for 3 hours, shaking mouse cages, reversing day and night, heating, overnight wet padding and overnight inclination of mouse cages at the temperature of 45 DEG C; the same kind of stimuli discontinuously appear, so that the animals cannot be subjected to unpredictable stimuli; 3 mg / kg of Isoprotenol hydrochloride is subcutaneously injected to the abdomen every day from the sixth week of moulding; and the injection is continued for 3 weeks. The method disclosed by the invention is high in operability, low in animal death rate, easy to evaluate, and low in cost, and is a dual-heart disease animal model with combined diseases and syndromes and intermingled phlegm and blood stasis syndromes.

The invention relates to a preparation method and application of bacillus antibacterial peptide. A Bacillus amylolique strain ES-2-4 used in the invention can produce antibacterial peptide WJ-1 which exerts antibacterial effect on Escherichia coli, Salmonella, Micrococcus luteus and Staphylococcus aureus and has a bacterium inhibition zone diameter of 20 to 30 mm. The Bacillus amylolique strain ES-2-4 undergoes inoculation, preparation of shake-flask seeds, production of primary seed liquid, secondary fermentation and solid-liquid separation; then obtained supernatant is mixed with a carrier and spray drying is carried out on the obtained mixture so as to obtain dried active bacterium powder; and carrier dilution is carried out so as to obtain a feed additive product. The additive is applicable to livestock and poultry in the whole course of culture, and can improve the productivity of livestock and poultry, promote growth of livestock and poultry, enhance immunity of livestock and poultry, reduce the mortality of young animals, decrease the excretion of manure, reduce the odor of manure and improve a culturing environment.

The invention discloses a construction method of an Adriamycin nephropathy animal model, for solving the technical problem that an existing Adriamycin nephropathy animal model is complex in operation and low in success rate. The construction comprises the following steps: selecting rats according with experimental standards as a molding group; injecting Adriamycin to the rats of the molding group by caudal vein for the first time according to a using amount of 4mg / kg; one week later, injecting Adriamycin for the second time according to the using amount of 3.5mg / kg; from the first injection of Adriamycin to the rats in the molding group, feeding the rats with high protein feed for two weeks, and then feeding with a normal feed for 6 days; and from the first injection of the Adriamycin to the 20th day, reckoning the rats with the total amount of 24-hour urine protein being larger than 30mg into the Adriamycin nephropathy animal model. The construction method disclosed by the invention is simple, feasible, high in success rate, good in consistency and stability and capable of effectively reducing the animal death rate and is a relatively ideal animal model for further researching sertoli cytopathy.

A method for creating the animal model of traumatic deep venous thrombosis in limbs features that the intramedullary nail driver is reformed to become a quantitative beater and said beater is used to beat the near ends of lower limbs of rat to obtain said animal model.

The invention discloses a method for powdery activation of plant residue and application thereof. The method comprises the steps of removing impurities in the plant residue, and obtaining plant residue blocks after crushing; adding water into the plant residue blocks, and adding hydrogen peroxide with stirring until no bubble is generated; adding strains, nitrogen source and cane molasses or beet molasses into the materials; standing at normal temperature and performing fermentation, wherein the fermentation period is 25 days or more, performing turning and stirring once every other 2-3 days during the period, and obtaining a plant residue powdery activated material after thorough decomposition. Waste is utilized, the plant residue powdery activated material manufactured by utilizing the method can be applied to animal feed to be matched with other feed additives, so animal organism immunity can be improved, and the animal death rate is reduced. The plant residue powdery activated material can further be matched with other fertilizers to be used as a bottom-applied organic fertilizer to be used for improving soil.

The present invention provides a method for establishing an animal model of radiation-induced colorectal inflammation. The method comprises the following steps: mice are randomly divided into two large groups; the mice are normal fed for 1-2 weeks to enable the mice to be adapted to a new living environment; body positions of the mice during irradiation are simulated and an average value is taken;a radiotherapy plan is developed; depending on different group designs, a single large dose radiation is conducted using different doses of gradient rays; and severity of colorectal tissue lesions isassessed. Combining clinical practices, a common therapeutic ray (6MV X ray) used by a medical linear accelerator can better simulate radioactive colorectal injury lesion process of clinical radiotherapy side reactions, and besides, the method is low in animal mortality and simple and easy to repeat, provides an experimental basis for studying the radioactive colorectal injury, is convenient forfurther studying mechanism of the radioactive colorectal injury and guiding clinical medication, closer to clinical practice and more conducive to studying course mechanism of radiation enteritis anddrug development, and can also further expand use of a prevention and treatment technology applied in the radiation enteritis.

The invention discloses a feed additive with two-way stress physiological regulation and a preparation method thereof. The feed additive comprises the following components by weight portions: 0-25 portions of a 2-benzanthrone derivative, 0-5 portions of a Gamma- aminobutyric acid derivative and 25-90 portions of defated rice bran. The feed additive has the beneficial effects of causing the animals to be more comfortable, reducing the aggressive behaviors, enhancing the appetite of animals, improving the feed intake, promoting the adaptability and disease resistance of the body and avoiding stress diarrhea, thus reaching the purposes of avoiding stress syndrome and improving the quality of animal products; meanwhile, two main components of the feed additive are plant extracts, which reaches the requirements of no residue and environmental protection. By applying the feed additive of the invention in raising poultry and livestock, the animal death rate is reduced by 5-15%, the feed intake is increased by 5-15% and the weight is increased by 5-15%. Compared with the anti-stress feed additives applied to the prior art, the feed additive of the invention obviously improves the effects.

The invention belongs to the field of livestock feeds, and particularly relates to a making method and application of a feed for ruminant animals. The making method of the feed for ruminant animals comprises the following steps of making composite microorganism bacterial liquid and enabling materials to be fermented with the composite microorganism bacterial liquid, so that silage is obtained. According to the preparation method of the feed for ruminant animals, provided by the invention, the composite microbial fermentation silage is adopted, so that the digestion and utilization rate of thesilage by the ruminant animals is increased, the intestinal flora of cattle is improved, diarrhoea is prevented, and nutrient absorption is promoted. The feed for the ruminant animals, provided by theinvention, has the advantages that the feed is natural, free from toxin and free from side effects, cannot cause drug tolerance, is free from environmental pollution, can promote growth and weight increment of animals, strengthen immune functions of organisms, reduce the mortality rate of the animals, increase the feed conversion rate and the animal feeding benefits.

The invention provides a strain of Bacillus subtilis DCU, the preservation number of which is CCTCC NO.2014118. The Bacillus subtilis not only has good anti-pathogenic activity, but also can well inhibit the growth and reproduction of pathogenic bacteria, and can also improve the body's Immunity has a wide application prospect. It can be applied to probiotic preparations, or it can be made into bacterial powder and added to feed as a feed additive to inhibit the growth of pathogenic bacteria in animals and reduce animal mortality.

The invention provides a transgenic crop risk assessment method based on mesofauna multi-generation observation. According to the method, mesofauna is raised in a culture medium with transgenic crop litter, survival rates, reproductive rates and body lengths of different generations of the mesofauna are counted, functional enzyme of the mesofauna is detected, and the influence of transgenic crops on continuous multi-generation mesofauna population fitness is comprehensively assessed. By improving a mesofauna culture medium formula, the culture medium is more adaptable to multi-generation continuous culture of the mesofauna by the transgenic crops. Besides, by monitoring response of acetylcholine esterase, superoxide dismutase, catalase and the like in the functional enzyme of the mesofauna to the transgenic crop litter, response of the mesofauna to the transgenic crops is comprehensively reflected according to parameters such as death rates and the survival rates of the mesofauna.

The invention provides a non-connection model building method for a hypertrophy bone connected with femoral shaft gristle of a big mouse. The method comprises: fixing a support outside the femora of the big mouse; fixing a catagmatic femoral shaft of the big mouse, and obtaining the non-connection model for the hypertrophy bone connected with the femoral shaft gristle of the big mouse. The non-connection model building method for the hypertrophy bone connected with the femoral shaft gristle of the big mouse has the advantages of being simple in operation, small in damage, low in animal mortality, fitting in non-connection clinical characters of the clinical hypertrophy bone, easy in operation, and scientific and reasonable. The non-connection model building method for the hypertrophy bone connected with the femoral shaft gristle of the big mouse has the great significance for the intensive study of the reason of the non-connection development of the hypertrophy bone, and the therapeutic method.

The invention discloses a construction method of an Adriamycin nephropathy animal model, for solving the technical problem that an existing Adriamycin nephropathy animal model is complex in operation and low in success rate. The construction comprises the following steps: selecting rats according with experimental standards as a molding group; injecting Adriamycin to the rats of the molding group by caudal vein for the first time according to a using amount of 4mg / kg; one week later, injecting Adriamycin for the second time according to the using amount of 3.5mg / kg; from the first injection of Adriamycin to the rats in the molding group, feeding the rats with high protein feed for two weeks, and then feeding with a normal feed for 6 days; and from the first injection of the Adriamycin to the 20th day, reckoning the rats with the total amount of 24-hour urine protein being larger than 30mg into the Adriamycin nephropathy animal model. The construction method disclosed by the invention is simple, feasible, high in success rate, good in consistency and stability and capable of effectively reducing the animal death rate and is a relatively ideal animal model for further researching sertoli cytopathy.

The invention relates to a real-time monitoring system for oxygen saturation of animal blood. The real-time monitoring system for oxygen saturation of animal blood comprises a detection part, a data processing module, a data storage module and a power supply. The detection part is used for being fixed to a specific detection part of an animal body so as to detect oxygen saturation information of animal blood in real time. The data processing module is connected to the detection part. The data storage module is connected to the data processing module for storing the processed blood oxygen saturation information. The power supply is used for supplying power to the detection part, the data processing module and the storage module. By using the real-time monitoring system for oxygen saturation of animal blood, the oxygen saturation of blood of the fed animals can be monitored in real time, so that workers can find the animals with abnormal bodies in time, and then the death rate of the animals in the animal breeding process can be reduced.

The invention belongs to the technical field of animal disease models, and specifically discloses an improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method. The improved SD rat CD modeling method comprises the following steps: after fasting and narcotizing an SD rat, carrying out clysis by using a modeling drug (which is prepared by mixing 5 percent of a TNBS (Trinitro-Benzene-Sulfonic Acid) water solution with absolute ethyl alcohol according to a volume ratio of 1 to 1 and is 0.2 ml / 100 g in body mass) according to the dosage of 50 mg / kg, adopting a body position of enabling the head of the SD rat to face downwards and the tail to face upwards after leading in the modeling drug so as to prevent clysis liquid from flowing outwards, and reversely carrying the SD rat for 1 to 2 minutes. The improved SD rat CD modeling method disclosed by the invention is simple and convenient in operation and good in repeatability, and a model accords with the features such as diarrhea, bloody stools, intestinal obstruction, weight lose, colonic ulcers and adhesion and abscess between intestinal canals and between an intestinal canal and visceral organs or tissues of clinic human CD; the modeling success rate is high, and the animal death rate is low.

The invention relates to a pharmaceutic preparation for preventing and treating arrhythmia and application thereof. The pharmaceutic preparation is prepared from common macrocarpium fruit, salviae miltiorrhizae, saffron, milkvetch roots, turmeric root tubers, rhizoma chuanxiong, common cephalanoplos herb, lotus seeds, fructus arctii, alpine swertia, cinnamon, corn stigmas, fructus schizandrae, licorice roots, polygonumaviculareL., gold silkworms, pear peels, honeysuckle flowers, cape jasmine fruit, Huai wheat, immature bitter oranges and poria cocos. According to the prescription, materials are selected scientifically, the pharmaceutic preparation prepared through the preparation method has the prominent function of preventing or treating the arrhythmia, and the pharmaceutic preparation can prominently prolong the occurrence time of rat arrhythmia ventricular beat, ventricular tachycardia and ventricular fibrillation caused by aconitine and reduce animal mortality. By means of acute toxicity tests and long term toxicity tests, the toxic and side effects are low, the safety of drug use can be obviously improved, and the pharmaceutic preparation for preventing and treating the arrhythmia and the application thereof are suitable for further application and promotion in clinic.

The present invention relates to uses of loganetin in preparation of drugs for prevention or treatment of arrhythmia, and belongs to the field of medicine. According to the present invention, the loganetin is the tradition Chinese medicine monomer extracted from the traditional Chinese medicine; the animal test results show that the loganetin can significantly prevent and treat arrhythmia, can significantly delay the occurrence of premature ventricualr contraction, ventricular tachycardia and ventricular fibrillation of arrhythmia rats due to aconitine, can significantly reduce the animal mortality, and can significantly inhibit the ventricular fibrillation incidence rate of chloroform-induced arrhythmia mice; and the loganetin has advantages of exact arrhythmia treatment effect, low side effect, and broad medical application prospect.

The invention discloses a method for making a novel kidney disease model. In particular, the renal artery of a mouse is occluded for 60 minutes, and the mouse can survive; renal pathological examination is performed on the 21st day, and chronic renal injury pathological changes such as diffuse fibrosis can be found. Compared with the prior art, the method has the advantages that after successful establishment of an animal model, an effective tool is provided for possible deep researches of the mechanism and intervention of AKI to CKD transformation in the field of kidney diseases, the modeling animal mortality is lowered greatly, and chronic renal injuries are formed effectively; the method has popularization and application values.

The present invention relates to uses of loganetin in preparation of drugs for prevention or treatment of arrhythmia, and belongs to the field of medicine. According to the present invention, the loganetin is the tradition Chinese medicine monomer extracted from the traditional Chinese medicine; the animal test results show that the loganetin can significantly prevent and treat arrhythmia, can significantly delay the occurrence of premature ventricualr contraction, ventricular tachycardia and ventricular fibrillation of arrhythmia rats due to aconitine, can significantly reduce the animal mortality, and can significantly inhibit the ventricular fibrillation incidence rate of chloroform-induced arrhythmia mice; and the loganetin has advantages of exact arrhythmia treatment effect, low side effect, and broad medical application prospect.

The invention provides novel application of fluoxetine and ziprasidone combination in preventing and treating central heat due to acute injury of central nervous system. For an animal with cerebral trauma, the fluoxetine and ziprasidone combination can improve central fever and control intracranial pressure rise due to cerebral fever, may effectively reduce apoptosis of cerebral injury focal peripheral nerves and reduce animal morality relevant to cerebral injury and is safe and free of significant toxic and side effects.

The invention discloses a method for detecting the curative effect of ornidazole for resisting against bacteroides fragilis infection. The method comprises the following steps: dividing mice into 13 groups evenly, 20 mice in each group; injecting 8*108CFU of bacteroides fragilis into the abdominal cavity of each mouse to infect the mouse, after infecting the mouse for 6 hours, injecting ornidazole into caudal veins of six groups of mice, injecting tinidazole into caudal veins of the other six groups of mice and injecting the equal quantity of saline into the rest group and using the rest group as a blank control group; and continuously observing the mice for seven days, recording the animal mortality and carrying out data processing. According to the method, analysis shows that the curative effect of ornidazole for resisting against bacteroides fragilis infection is equivalent to that of tinidazole, and then possibility is provided for replace tinidazole with ornidazole as a new drug for resisting anaerobes.

The invention provides a coronary artery microembolism animal model. According to the invention, the model is used for injecting the SDS normal saline suspension of the polystyrene microsphere into the apex of the left ventricle of an experimental animal while the aorta of the animal is clamped and closed at the same time. After that, the blood supply is restored again after a plurality of cardiaccycles of the aorta rising aorta are further kept. The coronary artery microembolism animal model has the advantages of being low in cost, low in animal death rate and high in simulation degree. By means of the model, an important research foundation is provided for preventing and treating coronary artery microembolism, the research and development of medicines and instruments for the coronary artery microembolism.