44 results about "Sterile water" patented technology

This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.

This invention discloses a high-dietary-fiber seaweed powder, its preparation method, and its application, belonging to the field of food technology. The high-dietary-fiber seaweed powder is prepared by the following method: (1) seaweed is soaked in slightly acidic electrolyzed water, ultrasonically cleaned, rinsed, dried, pulverized, and sieved to obtain coarse seaweed powder; (2) the coarse seaweed powder is added to a twin-screw extruder, and deionized water and a modifier are added simultaneously at the side feed port. After rotary cutting, granulation, and cooling, modified seaweed is obtained; (3) the modified seaweed is placed in a fermentation container, mixed with sterile water containing glucose, and fermented with a compound bacterial agent; after heat preservation, cooling, pre-freezing, vacuum freeze-drying, pulverization, and sieving, the seaweed powder is obtained. The seaweed powder prepared by this invention has a high total dietary fiber content and a high soluble dietary fiber content, excellent water-holding capacity, swelling capacity, and DPPH free radical scavenging rate, and a relatively weak fishy smell.

This invention discloses a method for improving the resistance to postharvest black spot disease in sweet potato tubers and maintaining their quality, belonging to the field of postharvest preservation technology for agricultural products. The method includes: selecting undamaged sweet potato tubers for callus treatment at room temperature for 48 hours, disinfecting with 1% sodium hypochlorite, rinsing with sterile water and air-drying, then soaking them in a 9.0 mmol / L benzothiadiazole (BTH) solution (containing 0.05% Tween 80) at room temperature for 60 minutes, removing them and air-drying them to complete the treatment. This invention induces the activation of the sweet potato tuber's own defense system through BTH, increasing the activity of key enzymes in phenylpropane metabolism, chitinase and other disease-resistant enzymes, promoting the accumulation of total phenols and lignin, maintaining cell membrane integrity, inhibiting starch degradation and abnormal increase in soluble sugars. Under storage conditions of 28±1℃ and 80-85%RH, it can significantly inhibit the expansion of black spot disease lesions and extend the storage period to more than 25 days. This method has no pesticide residues, is unlikely to cause pathogen resistance, is environmentally friendly, simple to operate, and cost-controllable, and is suitable for large-scale postharvest preservation of sweet potato tubers.

This utility model relates to the field of steam ablation technology, providing a prostate thermal steam ablation catheter and steam ablation handle. The catheter includes a guide rod and a catheter tip, with the catheter tip located at the end of the guide rod. The catheter tip has a needle insertion channel and a flushing channel. The catheter tip has a first notch, a second notch, and a third notch, which surround the needle insertion channel. The sidewall of the catheter tip also has a first shielding surface and a second shielding surface. The first shielding surface guides a portion of the fluid flowing out along the first notch to flow in the opposite direction, and the second shielding surface guides a portion of the fluid flowing out along the second notch to flow in the opposite direction. This structure guides the flow of sterile water to the steam ablation center corresponding to the puncture needle, ensuring that sterile water covers three sides of the steam ablation center, forming a vortex. This allows for heat dissipation and uniform cooling of the treatment area, helping to avoid the risk of steam burns to tissue and improving the clarity of observation of the treatment area.

The application discloses a low-irritation borage oil nano-liposome, a diaper-care preparation and application of the diaper-care preparation in diaper rash of infants and young children, and belongs to the field of infant skin-care preparations. The nano-liposome is composed of the following components in percentage by weight: 0.5-2.0% of biodecolorized borage oil, 1.0-3.0% of chitosan oligosaccharide-ceramide binary membrane material, 0.3-1.0% of prebiotic skin flora regulator, 0.8-1.5% of pH 5.5 weak acid buffer system, 0.05-0.2% of edible-grade microencapsulated menthol, and the balance of sterile water for injection. The application also provides a diaper-care preparation containing the low-irritation borage oil nano-liposome, which is suitable for daily prevention and acute-phase intervention of infants and young children aged 0-36 months, has the characteristics of high bioavailability, low irritation, rapid repair and easy carrying, and has important clinical application value.

The present application relates to the technical field of plant tissue culture, and specifically discloses a method for establishing a tissue culture sterile system of peach rootstock dormancy material, which comprises the following steps: S1, selecting one-year-old branches with leaf buds in the dormancy period, removing flower buds and cleaning; S2, performing low-temperature treatment on the branches, then performing water culture and bud breaking to obtain sprouting branches; S3, performing surface sterilization treatment on the sprouting branches, and then washing with sterile water; S4, peeling the sprouting buds from the branches under sterile conditions, removing the external wrapping tissues of the bud bodies, and exposing the internal tender tissues; S5, performing secondary sterilization treatment on the exposed tender tissues, and then washing with sterile water; and S6, inoculating the bud bodies subjected to the secondary sterilization treatment into a culture medium for culture, and establishing a sterile system; according to the above method, in the process of establishing the sterile system of Zhongtao Kangzhen No. 1 material, the sterilization survival rate of 100 bud heads is as high as 80% or more, and the work efficiency and the utilization efficiency of the material are greatly improved.

This invention discloses an anti-fogging structure for gastrointestinal endoscope lenses, belonging to the field of endoscopy technology. The anti-fogging structure includes an instrument body. A controller, adjustment mechanism, and water supply mechanism are fixedly mounted on the surface of the instrument body. A connecting tube is fixedly connected to the surface of the instrument body, and a probe is fixedly connected to the end of the connecting tube. The connecting tube and the probe are used to insert into human organs. The adjustment mechanism is used to adjust the position of the probe. The lens body is fixedly mounted on the surface of the probe. A heating tube is tightly fitted to the surface of the lens body, forming a highly efficient heat conduction structure. When sterile water is delivered to the heating tube through the outlet tube, the heating tube rapidly heats up and evenly transfers heat to the surface of the lens body, avoiding blurred vision caused by fogging. Furthermore, using sterile water as the medium avoids the risk of secondary contamination and meets medical-grade safety standards, balancing functionality and hygiene requirements.

When the COP processing and the SOP processing in the chamber of the aseptic filling machine are performed, the cleaning solution or the sterile water remaining in the chamber before the sterilizing agent containing peracetic acid or hydrogen peroxide is sprayed is promptly removed. The conveying device that conveys the container in the chamber of the aseptic filling machine is driven to remove the cleaning solution after the COP processing or the sterile water during the SOP processing.

The utility model discloses a condensing drying type endoscope cleaning disinfection sterilizer, be applicable to all kinds of endoscope high level disinfection or sterilization, and the machine is by refrigeration cabin, cleaning disinfection cabin, functional assembly cabin and liquid storage cabin composition, and each cabin component is connected through pipeline, wire and constitutes the cleaning system, disinfection system, condensing drying system and real -time detection system, under the control of intelligent module, the machine realizes cleaning agent disinfectant stock solution dilution, spray soaking cleaning disinfection, clean water washing cleaning agent residue, sterile water washing disinfectant residue, sterile air internal circulation condensing drying, cleaning agent disinfectant waste liquid discharge and the whole process automation operation of wet heat self -cleaning disinfection, can carry out real -time detection regulation and control to the concentration temperature of cleaning agent, disinfectant and rinsing water during, and through cleaning agent, disinfectant and rinsing water staggered independent circulation path and separate tank storage, carry out cleaning agent, disinfectant recycling and reuse, can also print and store disinfection record simultaneously, provide data traceability support for hospital infection prevention and control.

This invention belongs to the field of avocado disease resistance screening technology, specifically relating to a method for screening rootstocks resistant to avocado root rot, comprising the following steps: S1. Selecting plump, disease-free avocado seeds to be screened, disinfecting them, and then hydroponically cultivating them to obtain avocado hydroponic seedlings; S2. Activating and propagating the avocado root rot pathogen and preparing a mycelial suspension; S3. Rinsing the roots of the avocado hydroponic seedlings with distilled water and placing them in sterile hydroponic cups containing the mycelial suspension for cultivation and observation; S4. Grading and investigating the disease incidence of the avocado hydroponic seedlings and calculating the disease index (DI); S5. Classifying the resistance level according to the disease index (DI). This invention, by establishing a standardized hydroponic inoculation identification method, successfully overcomes the inherent defects of traditional methods, achieving significant progress in screening efficiency, result accuracy, and operational feasibility, providing efficient, reliable, and strong support for avocado root rot resistance breeding.

The present application belongs to the technical field of plant tissue culture, and particularly relates to an active ventilation tissue culture device and a use method. The tissue culture device comprises: a gas filling pump connected with a controller to control start and stop; a gas washing bottle with sterile water inside; an air inlet pipe of the gas washing bottle connected with the gas filling pump through an air guide pipe, and the other end of the air inlet pipe extends below the liquid level of the sterile water in the bottle; a plurality of tissue culture bottles connected in series by the air guide pipes; a tissue culture bottle at the end of the series connected by the air guide pipe for air exhaust; and air filters arranged on the air guide pipes between the gas washing bottle and the gas filling pump, on the air guide pipes between the tissue culture bottles, and on the air guide pipes for air exhaust. The serially connected passages make the air in the tissue culture bottles flow, reduce the accumulation of chemical substances, regulate and control the coordination between photosynthesis and respiration of the plants, and promote the normal and healthy growth of the plants.

The utility model discloses an integrated formula high -efficient even distribution aeration's ultraviolet disinfection sterile water tank, including water tank main part, is equipped with aeration structure in it, and this aeration structure includes aeration tray, support frame, gas outlet rod, guide pipe, breathable membrane, catheter and aeration pump. Aeration tray and gas outlet rod surface all are equipped with the gas outlet hole, and the gas outlet hole surface is connected with the breathable membrane, is used for producing small, even bubble, improves aeration efficiency. The water tank main part top fixed connection has ultraviolet disinfection lamp tube, and disinfects to the water body. The utility model integrates aeration and ultraviolet disinfection function, and the structure is compact, improves aeration evenness and disinfection efficiency, reduces equipment cost and installation complexity, and the operation is simple, can further integrate ozone generator, and promotes the processing effect.

This invention proposes a culture medium and cultivation method for Tremella fuciformis, belonging to the field of Tremella fuciformis cultivation technology. It is prepared from the following raw materials in parts by weight: 0.5-1 parts antibacterial agent, 5-7 parts traditional Chinese medicine residue, 3-5 parts sugarcane bagasse, 2-3 parts fermentation extract, 30-40 parts cottonseed hulls, 7-10 parts wheat bran, 0.5-1 part gypsum, and 50-60 parts sterile water. The Tremella fuciformis culture medium obtained by this invention can significantly inhibit the growth of pathogenic bacteria during cultivation, reduce the pathogenicity rate, and increase the yield of Tremella fuciformis. Simultaneously, it increases the content and efficacy of active substances in Tremella fuciformis, producing selenium-enriched Tremella fuciformis polysaccharide. The active substances exhibit excellent antioxidant, anti-inflammatory, anti-tumor, moisturizing, hypoglycemic, hypolipidemic, and cardiovascular protective effects. Using this invention's culture medium, along with a suitable ratio of Tremella fuciformis and Clematis chinensis symbiotically, provides a new method and material for Tremella fuciformis cultivation, resulting in Tremella fuciformis with better utilization value and broad application prospects.

This utility model discloses a multi-functional cap for endoscope irrigation water bottles, relating to the field of medical device technology. The cap comprises an interface, a screw-on cap body, and a guide post. The screw-on cap body has a through hole at its center, the interface is located at the external through hole of the cap body, and the guide post is located at the internal through hole of the cap body. The interface surface has external threads, and the inner surface of the cap body has internal threads along the periphery of the guide post. This multi-functional cap for endoscope irrigation water bottles is placed at the mouth of a bottle containing sterile water or other solutions. The interface connects to other external interfaces, including various interfaces with multi-functional tubing. It not only provides a good seal but also is compatible with various caps, making it convenient to use and more suitable for widespread adoption.

The present application relates to Ganoderma lucidum polysaccharide fermentation technical field, disclose a kind of fermentation process for improving the content of ganoderma polysaccharide and its fermentation product and application, including the following steps: using gradient mixing method, gradually mix ganoderma lucidum powder and aspergillus niger according to certain proportion, static culture fermentation in incubator, obtain aspergillus niger ganoderma fermentation product.Add sterile water and glucose to aspergillus niger ganoderma fermentation product, obtain mixed solution, after stirring evenly, inoculate rhamnose lactose casein bacillus liquid and lactobacillus plantarum liquid in sterile clean bench, after mixing evenly, transfer to sterile fermentation bag, constant temperature vibration fermentation obtains mixed fermentation liquor, mixed fermentation liquor freeze-drying is obtained, and mixed fermentation freeze-dried powder is obtained.The fermentation process of the present application effectively improves the content of main active substance in ganoderma fermentation product and its antioxidant activity, anti-inflammatory activity and bioavailability, effectively improves the symptoms of mouse ulcerative colitis;For exploring the application of fermented ganoderma in the development of functional food and drug provides a theoretical basis.

The present application belongs to the technical field of fermented food preparation, and particularly relates to a preparation method of red rice Euryale ferox powder and application thereof. The method comprises the following steps: inoculating Monascus into a PDA slant, culturing until the slant is covered with mycelium and spores, washing out the spores with sterile water after the culture is completed to obtain a spore solution; soaking dry Euryale ferox to a state capable of being kneaded, drying and sterilizing to obtain Euryale ferox material; inoculating the spore solution into the Euryale ferox material, and then performing fermentation; drying the fermentation product after the fermentation is completed to obtain red rice Euryale ferox, and screening after crushing to obtain the red rice Euryale ferox powder. The present application provides a preparation method of red rice Euryale ferox powder taking Euryale ferox as a new type of fermentation substrate, and successfully develops a high-value-added new product of deep processing of Euryale ferox. Through Monascus fermentation, two types of active ingredients of red rice and Euryale ferox are enriched, and a synergistic effect possibly generated is explored, thereby creating a compound functional food.

The invention discloses a probiotic fermentation method for improving total saponins of astragalus membranaceus based on response surface optimization, which comprises the following steps: inoculating a fermentation seed solution into a fermentation substrate containing astragalus membranaceus, and carrying out shake culture for 48 hours under the conditions of 40-45 DEG C and 150-200r / min; the inoculation amount is 1-5%; the fermentation seed liquid is obtained by culturing lactobacillus delbrueckii subsp. Bulgaricus CICC 20247; the fermentation substrate containing astragalus membranaceus comprises astragalus membranaceus powder, skim milk powder, white granulated sugar, yeast extract and sterile water. After the lactobacillus delbrueckii subsp. Bulgaricus CICC20247 liquid is used for fermenting the astragalus membranaceus, the content of total saponins in the astragalus membranaceus can be greatly increased, and the medicine effect of the astragalus membranaceus can be improved.

The present application relates to the technical field of medical devices, in particular to a steam ablation system and a control method thereof. The steam ablation system comprises a steam device, a sterile water delivery device, a pressure relief device and an ablation needle. The sterile water delivery device is used to introduce sterile water into the steam device at a constant flow rate. The ablation needle can be pushed out or withdrawn relative to the steam device. When the ablation needle is pushed out relative to the steam device, the steam discharged by the steam device is discharged through the ablation needle to achieve thermal ablation on the tissue. When the ablation needle is withdrawn relative to the steam device, the steam discharged by the steam device can be discharged through the pressure relief device. During the treatment interval, excessive steam is prevented from being discharged through the ablation needle to scald the patient. Since the sterile water delivery device always introduces sterile water into the steam device at a constant flow rate, the treatment efficiency is improved, and the occurrence of negative pressure at the ablation needle site is also avoided. The steam ablation system control method improves the treatment efficiency and avoids the occurrence of negative pressure at the ablation needle site.

The application discloses lactobacillus degrading hippophae rhamnoides malic acid and application thereof in preparation of hippophae rhamnoides juice, wherein one strain is classified and named as lactobacillus acidophilus (Lactobacillus acidophilus) Lactobacillus acidipiscis ) with a preservation number of CGMCC No. 37351, and one strain is classified and named as lactobacillus plantarum (Lactobacillus plantarum) Lactobacillus plantarum ) with a preservation number of CGMCC No. 37352. Fresh hippophae rhamnoides juice is mixed with sterile water according to a proportion, pasteurized after sugar degree is adjusted, cooled to room temperature, and pretreated; the lactobacillus is inoculated into the pretreated hippophae rhamnoides juice after being cultured to a logarithmic growth phase, and anaerobically cultured at 35±1 DEG C for 90-96 h. The lactobacillus screened in the application has a remarkable degradation effect on hippophae rhamnoides malic acid, can efficiently degrade hippophae rhamnoides malic acid, and meanwhile retains nutrition function and natural flavor, and can be used for preparation of hippophae rhamnoides juice.

This invention provides a hydroponic method for symbiotic cultivation of fritillaria and flowers, belonging to the field of soilless fritillaria cultivation technology. The hydroponic method includes the following steps: 1) Selecting healthy fritillaria bulbs, removing the dried surface tissue and fibrous roots, rinsing repeatedly with sterile water until no soil residue remains, and drying for later use; 2) Root-promoting treatment of the symbiotic flowers: Before hydroponic cultivation of fritillaria and flowers, the symbiotic flowers are first cultured in a hydroponic device with added nutrient solution to promote root growth; 3) After the symbiotic flowers have rooted, the fritillaria is then transplanted into the hydroponic device for symbiotic hydroponic growth, with nutrient solution added and replaced regularly until the fritillaria is ready for harvest. This hydroponic method significantly improves the survival rate of fritillaria in hydroponic cultivation. Through the "leading effect" of the symbiotic flower root system, the average survival rate of fritillaria is increased from approximately 60% in single hydroponics to over 90%.

This invention provides a method for aging small green tangerines and its application, belonging to the field of food processing technology. The method includes the following steps: after washing and removing the pulp from the small green tangerine peel, spraying it with inoculated *Pancreatoblasticulata* (a type of bacteria). Pantoea agglomerans The Z05 bacterial suspension was fermented at 25-30℃ for 12-24 hours. After fermentation, the product was rinsed with sterile water, steamed to inactivate, and dried to obtain aged small green tangerine peel. The Z05 bacterial clump is an endophytic bacterium in the small green tangerine peel, with the preservation number CGMCC No. 36512. This invention can complete the aging process that traditional processes require more than one year in 24 hours, increasing the aging efficiency by 300 times. The fermentation treatment significantly reduces the pungent odor of the peel, enhances the aged aroma, promotes the color change of the peel to dark brown, and maintains the flavonoid content and antioxidant activity. This invention has a simple process, short cycle, and stable effects, and is suitable for preparing products such as small green tangerine Pu-erh tea.

We will provide a new vaccine to prevent TB. [Solution] The present invention relates to a freeze-dried composition comprising a Mycobacterium tuberculosis complex, preferably a clinical isolate of M. tuberculosis, more preferably a clinical isolate of M. tuberculosis (MTBVAC strain), characterized by comprising a PhoP-phenotype due to inactivation by genetic deletion of the Rv0757 gene and a PDIM-phenotype due to deletion of a second gene Rv2930 (fadD26) that inhibits PDIM production, and sucrose and sodium glutamate as stabilizers or excipients. Furthermore, the present invention relates to a reconstituted composition obtained by adding water, preferably sterile water for injection, to the freeze-dried composition. The present invention relates to the composition to be used as a prophylactic agent for people at risk of infection with M. tuberculosis, or as a secondary drug for treating infected tuberculosis patients.

This invention discloses a Pseudomonas aeruginosa reducing agent and its application and method in vanadium reduction. Pseudomonas bariensis is inoculated into a vanadium-containing solution to reduce pentavalent vanadium ions. The concentration of pentavalent vanadium in the vanadium-containing solution is 0.5–10 mM. The vanadium-containing solution also includes high-salt LB medium, which is composed of: 10.0 g / L tryptone, 5.0 g / L yeast extract, and 30.0 g / L NaCl, prepared with sterile water. The pH is adjusted to 7.0 with 1 mol / L NaOH or 1 mol / L HCl, and the medium is autoclaved at 121°C for 20 min before use. The reduction reaction of the Pseudomonas aeruginosa and the vanadium-containing solution is carried out at 28°C, 150 rpm oscillation frequency, under anaerobic and dark conditions. The *Pseudomonas baralicum* provided by this invention can still grow when exposed to concentrations up to 10 mM V(V). When the vanadium concentration is 0.5, 1.0, 2.0, 5.0, and 10 mM, the reduction rate of V(V) by this *Pseudomonas baralicum* is 81.1%, 72.4%, 72.3%, 70.8%, and 60.6%, respectively, indicating that *Pseudomonas baralicum* can be used as a reducing agent to remove vanadium toxicity from vanadium-contaminated environments.

The application provides a postharvest preservation method of mango, which comprises the following steps: S1. picking and pretreatment, S2. short-wave ultraviolet mild irradiation treatment, S3. preservative solution immersion and storage. The preservative solution is composed of phyllium extract solution, mango leaf extract solution, arginine grafted modified chitosan, glacial acetic acid and sterile water. Through the synergistic process of the short-wave ultraviolet mild irradiation and the preservative solution immersion, combined with the multiple effects of the modified chitosan coating and the plant extract, the mango can be stored for 30 days under the condition of 13-15 DEG C and 85-90% relative humidity, the good fruit rate is still kept to be more than 90%, and the weight loss rate is less than 5%, so that the industry problems that the mango is prone to rot and fast water loss after harvesting are effectively solved. The components of the preservative solution used in the application are all safe natural sources, no chemical synthetic fungicides are used in the whole process, there is no pesticide residue risk, and the green preservation and food safety requirements are met.

The application discloses a coating liquid for enteric bacterial microcapsules and a preparation method and application thereof, relates to the technical field of coating liquids, and discloses the following steps: adding modified montmorillonite into distilled water, adding chitosan quaternary ammonium salt, reacting, centrifuging, cleaning, and drying to obtain chitosan quaternary ammonium salt modified montmorillonite; adding sodium alginate and modified carboxymethyl cellulose into sterile water, uniformly mixing, and obtaining coating liquid A; adding calcium chloride into sterile water, uniformly mixing, adding chitosan quaternary ammonium salt modified montmorillonite, and ultrasonically treating to obtain coating liquid B. The coating liquid is prepared, the coating liquid comprises the coating liquid A and the coating liquid B, bacterial suspension is added into the coating liquid A, the coating liquid B is further added, calcium ions in the calcium chloride in the coating liquid B are crosslinked with the sodium alginate to form a sodium alginate layer, the modified carboxymethyl cellulose and the modified montmorillonite improve the mechanical strength of the sodium alginate layer through interaction, and the embedding rate of the microcapsules is improved.

This invention provides a method for aging small green tangerines and its application, belonging to the field of food processing technology. The method includes the following steps: after washing and removing the pulp from the small green tangerine peel, spraying it with inoculated *Pancreatoblasticulata* (a type of bacteria). Pantoea agglomerans The Z05 bacterial suspension was fermented at 25-30℃ for 12-24 hours. After fermentation, the product was rinsed with sterile water, steamed to inactivate, and dried to obtain aged small green tangerine peel. The Z05 bacterial clump is an endophytic bacterium in the small green tangerine peel, with the preservation number CGMCC No. 36512. This invention can complete the aging process that traditional processes require more than one year in 24 hours, increasing the aging efficiency by 300 times. The fermentation treatment significantly reduces the pungent odor of the peel, enhances the aged aroma, promotes the color change of the peel to dark brown, and maintains the flavonoid content and antioxidant activity. This invention has a simple process, short cycle, and stable effects, and is suitable for preparing products such as small green tangerine Pu-erh tea.