538 results about "Sterile water" patented technology

The invention discloses banana resistant starch as well as a preparation method and application thereof in preparation of health-care food, and relates to the technical field of biology. The preparation method comprises the following steps: peeling immature bananas, performing irradiation disinfection, adding sterile water, performing grinding and pulping, and performing sieving with a 60-mesh sieve so as to obtain slurry; inoculating lactobacillus brevis and kluffa yeast into the slurry, and performing fermentation treatment to obtain fermentation liquor; standing the fermentation liquor, removing the supernatant, cleaning the precipitate with sterile water, and drying to obtain the banana resistant starch. The banana resistant starch prepared by performing combined fermentation treatment on lactobacillus brevis and Kluffa yeast can remarkably enhance the blood glucose metabolism regulation capability under the influence of high fat, so that the banana resistant starch can be applied to preparation of health-care food beneficial to maintaining the blood glucose health level. The method has important practical application prospects and is particularly suitable for market application and popularization.

The preparation method comprises the following steps: mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a ratio of 1: 1: 1 to obtain mixed bacteria; 0.25 g of mixed bacteria is taken and dissolved in 5 mL of sterile water for standby application; slicing and crushing the dried rhizoma polygonati, and sieving with a 10-mesh sieve to obtain rhizoma polygonati powder; the preparation method comprises the following steps: mixing water and rhizoma polygonati powder according to a liquid-material ratio of 10mL: 1g, sterilizing at 121 DEG C for 30min, cooling, adding mixed bacteria according to an inoculum size of 3% of the mass of rhizoma polygonati, culturing in a constant-temperature shaking table incubator at 120r. Min <-1 > for 72h at the culture temperature of 035 DEG C, sterilizing at 100 DEG C for 30min after fermentation, cooling and centrifuging to obtain rhizoma polygonati fermentation liquor, and storing at 4 DEG C. The prepared polygonatum sibiricum fermentation liquor is high in active ingredient content, has good cell safety, anti-oxidation, anti-inflammatory and whitening effects, and has the potential of being used for developing functional raw materials of traditional Chinese medicine cosmetics.

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

The invention discloses a growth regulator for promoting colonization of plant rhizosphere microorganisms and application of the growth regulator to crops, a preparation method of the growth regulator comprises the following steps: S1, dropwise adding an aqueous solution of modified chitosan and a compound bacteria solution into a saturated boric acid solution of 1-2% calcium chloride, crosslinking and curing for 3-4 hours, washing with sterile water for 3-5 times, and drying to obtain the growth regulator for promoting colonization of the plant rhizosphere microorganisms; immobilized microorganisms are obtained; s2, sequentially adding nanocellulose crystals, sodium polyaspartate and organic fertilizer into the enteromorpha extracting solution, uniformly stirring and mixing, adding immobilized microorganisms, uniformly mixing, granulating, coating with modified chitosan, and drying to obtain the growth regulator. Through step-by-step design of microorganism immobilization, nutrition compounding and chitosan coating, function integration of microorganism protection, nutrition release and environmental response is achieved, the colonization efficiency of rhizosphere microorganisms is remarkably improved, the soil microenvironment is improved, and finally comprehensive improvement of plant growth and stress resistance is achieved; and the method has relatively high social use value and application prospect.

The invention discloses a tissue culture method of a double-petal hemerocallis fulva scapes, which comprises the following steps: taking a double-petal hemerocallis fulva scapes, taking a growing point as a center, transversely cutting into long sections which are not less than 5cm, washing and disinfecting; putting the disinfected long segments into enzymatic hydrolysate for cell wall enzymolysis, taking out the long segments after enzymolysis is completed, and washing the long segments with sterile water; transversely cutting the long section subjected to enzymolysis into small sections of 1-2cm by taking the growing point as the center; and carrying out tissue culture by taking the small sections as explants. The method applies the double-petal hemerocallis fulva variety, and has the advantages that materials can be taken at any time in the flowering phase, the tissue culture efficiency is high, and the quality is good.

The invention provides a liquid fertilizer for improving tomato fruit saccharic acid quality and application, and belongs to the technical field of fertilizers. The liquid fertilizer for improving the tomato fruit saccharic acid quality provided by the invention is prepared from the following components in percentage by weight: 42 to 47 percent of organic nutrient solution, 10 to 15 percent of water-soluble macroelement compound, 1.5 to 2.5 percent of natural organic acid, 3.5 to 4.5 percent of plant source amino acid compound, 3 to 5 percent of compound biostimulant, 0.02 to 0.07 percent of polyglutamic acid and 0.2 to 0.4 percent of sugar alcohol medium trace element compound liquid, and 28-33% of sterile water. Through optimized formula design, the organic liquid fertilizer is rich in plant nutrition and bioactive substances, can effectively improve the healthy growth state of tomatoes, and meanwhile, remarkably improves the fruit quality.

The invention discloses a soil carbon sequestration microorganism isolated culture method under medium and high petroleum pollution stress and application, and the isolated culture method comprises the following steps: dispersing medium and high petroleum pollution soil surface soil in sterile water to obtain an original bacterial suspension containing carbon sequestration microorganisms, and carrying out gradient dilution on the original bacterial suspension; respectively coating on a carbon-source-free solid culture medium for culturing, purifying a bacterial colony obtained by culturing, and carrying out step-by-step amplification culture on a single bacterial colony obtained by purifying in a carbon-source-free liquid culture medium. The method has the beneficial effects that the invention provides the method for enriching and screening the indigenous indigenous efficient carbon sequestration strains with the carbon sequestration function from the medium-high petroleum pollution soil sample, and the four strains which can survive in the medium-high petroleum pollution stress environment and have good carbon dioxide sequestration capability are obtained through the method; the strains have potential pollution reduction and carbon sequestration synergistic capability, and a new technical path can be provided for green remediation and carbon sequestration of petroleum-contaminated soil.

The invention discloses fermented lactobacillus mucus Z-15 and application thereof in preparation of fermented bread, belongs to the technical field of microorganisms, and provides the fermented lactobacillus mucus Z-15 which is preserved in the China Center for Type Culture Collection on January 5, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2024024. The method for preparing the fermented bread comprises the following steps: mixing the fermented lactobacillus mucus Z-15, sterile water and high gluten flour, and standing to obtain sour dough; mixing with high gluten flour, kluyveromyces marxianus GY-8, white granulated sugar, salt and water, then adding butter, standing for fermentation, performing secondary fermentation to obtain bread dough, and baking to obtain the fermented bread. According to the method disclosed by the invention, the flavor of the bread obtained by co-fermentation of the lactobacillus mucus Z-15 and the kluyveromyces marxianus GY-8 is remarkably improved.

The invention relates to the field of microorganism sampling detection, in particular to escherichia coli sampling detection equipment for mouse experiments. The technical problem to be solved is that when mouse intestinal escherichia coli is sampled and detected, attention needs to be highly concentrated during manual operation, physical and mental exhaustion is easily caused, and the intestinal tract is damaged by slight carelessness. In addition, in the prior art, adipose tissue existing on the outer surface of the intestinal tract cannot be removed, and therefore the sampling detection result is affected. According to the technical scheme, the escherichia coli sampling and detecting equipment for the mouse experiment comprises a sampling box, a fixing assembly and the like; and a fixing assembly is mounted in the sampling box. According to the device, the left end and the right end of the intestinal tract of a mouse are fixed through the first clamping block and the second clamping block respectively, sterile water is injected into the intestinal tract of the mouse through an injection pipe for flushing, and cleaning of the intestinal tract of the mouse is completed, so that the pressure of manual operation is relieved, and meanwhile accidental damage to the intestinal tract of the mouse caused by manual operation is reduced.

The invention discloses bacillus simplex A16 which is registered and preserved in the China Center for Type Culture Collection on July 18, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241597. The application of the bacillus simplex in uranium enrichment comprises the following steps: inoculating a ring of bacillus simplex into a peptone fermentation culture medium, and culturing in a shaking table for 20-28 hours to obtain a bacterial solution with an OD600 value of 1.6-2.0; centrifuging the obtained bacterial liquid, discarding the supernatant, and washing the precipitate with sterile water for 2-4 times to obtain thalli; and under the aerobic condition, adding the obtained thalli into a uranium solution, and culturing in a shaking table for 2-24 hours. The invention provides bacillus simplex A16, which can quickly remove uranyl ions in a water body, effectively reduce the concentration of uranium and achieve the purpose of repairing the uranium-polluted water body.

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention discloses an anti-flocculation defoaming agent for digestive tract mucosa in gastroscopy and a preparation method of the anti-flocculation defoaming agent, and relates to the technical field of medicines, and the anti-flocculation defoaming agent comprises simethicone emulsion, sodium bicarbonate and sterilized water for injection. 80mg of simethicone emulsion is combined with 40ml of 2.5% sodium bicarbonate solution (containing 1000mg of sodium bicarbonate) to be used for preparation before gastroscopy, flocculent precipitation can be prevented and the gastric mucosa cleaning effect can be improved by using a small dose of simethicone emulsion, and the simethicone emulsion is simple to use, can be rapidly prepared, is low in cost and high in cost performance, and reduces the medical cost.

The utility model relates to a medical sterile water preparation device which comprises a water distribution structure and water preparation and storage equipment, the water distribution structure comprises a water inlet and a plurality of water outlets, and the water inlet is connected with a water source; the water production and storage equipment is provided with a plurality of water production and storage units, each water production and storage unit is provided with a volume cavity, a water inlet end and a water outlet end, the water inlet end and the water outlet end are communicated with the volume cavity, and independent valves are arranged at the water inlet ends and the water outlet ends of the water production and storage units. The water inlet ends of the water producing and storing units are connected with the water outlets in the water distributing structure in a one-to-one correspondence mode through pipelines, the water storing and using equipment further comprises a main water outlet, and the front end of the main water outlet is correspondingly connected with the water outlet ends of the water producing and storing units through parallel pipelines; and an ultraviolet lamp tube for preparing sterile water is arranged in the volume cavity in each water preparation and storage unit. The device has the advantages of high efficiency, safety, convenience, intelligence, space conservation, energy conservation, environmental protection and the like, and reliable guarantee is provided for preparation and application of medical sterile water.

The invention discloses a preparation method of monensin fermentation liquor, relates to the technical field of microbial fermentation, and aims to solve the problems of low titer and long period of the existing process. According to the method, streptomyces cinnamomi is taken as a strain, a culture medium with a specific formula is adopted, and fermentation is performed in three stages: in the first stage (0-30-50 hours), pH is not controlled; in the second stage, a phosphoric acid and compound oil mixed solution (accounting for 1-3% of the fermentation solution, and the compound oil contains 20-30% of peanut oil, 20-30% of corn oil and the like) is supplemented at a time; in the third stage, the pH is controlled to be 6.2-6.3, and soybean oil and sterile water are supplemented according to needs. The fermentation adopts a secondary process, the temperature is controlled at 30-33 DEG C, the dissolved oxygen is more than or equal to 35% and the like, the total duration is less than or equal to 320 hours, and the titer is more than or equal to 55000 mu g / ml. By optimizing the culture medium and precisely regulating and controlling, the element production capacity is improved, the method is suitable for industrial production of tanks of 50 L to 100 m < 3 >, the efficiency is improved, and the cost is reduced.

The invention discloses a cryoprotectant which comprises glycerol, skimmed milk powder, mannitol, trehalose, vitamin C, fructo-oligosaccharide, inulin and sterile water. By optimizing the components of the cryoprotectant, the activity of the coprophilous fungi can be effectively protected, the survival rate of the intestinal coprophilous fungi in the freezing process is remarkably increased, all the used components are food-grade edible components and have a wide application range and economic effects, the invention further discloses a coprophilous fungi freezing preservation method adopting the cryoprotectant, and the cryoprotectant has good application prospects. The method is simple, easy to operate and suitable for large-scale production and application.

The invention discloses a construction process for treating endangered haloxylon ammodendron trees by using a microbial technology. The construction process aims at solving the problem that withered haloxylon ammodendron trees cannot be rejuvenated and gradually die under the condition that a large amount of water cannot be watered in an extremely arid area. Comprising the following steps: step 1, preparing haloxylon ammodendron microbial bacterial liquid; step 2, preparing microbial minerals; step 4, backfilling microbial minerals; step 5, irrigating haloxylon ammodendron with microbial bacterial liquid; 6, backfilling the treatment pits with soil; the haloxylon ammodendron is irrigated for the first time for 5-7 days, the haloxylon ammodendron is irrigated for the second time, the haloxylon ammodendron microorganism bacterium liquid prepared according to the specification and sterile water are mixed according to the proportion of 1: 500, the mixture is put into a watering cart for water irrigation and then irrigated on site, and each haloxylon ammodendron is irrigated with 20 kg of bacterium liquid. The process realizes water-saving rejuvenation of haloxylon ammodendron trees in extreme drought and water-deficient areas by using a microbial technology, and is low in cost, high in efficiency, good in ecological benefit and water-saving.

The invention discloses preparation and application of an umbilical cord extract capable of blacking hair by regulating melanocytes of hair follicles to generate melanin particles. The preparation method of the umbilical cord extract comprises the following steps: washing an animal umbilical cord, cutting the animal umbilical cord into pieces, digesting the animal umbilical cord with pancreatin, adding a serum-free mesenchymal stem cell culture medium, blowing and beating to disperse tissues, filtering, performing low-energy red light irradiation on the filtered tissue fluid, incubating for 6-12 hours, taking out the tissue fluid, performing freeze thawing treatment, performing ultrasonic treatment, filtering, diluting the filtrate with sterile water, and performing freeze drying to obtain the umbilical cord extract. The umbilical cord extract is obtained. The umbilical cord extract disclosed by the invention has a relatively good regulating effect on melanocyte activity and melanin generation capability, can increase the content of melanin particles in hair, and can be used as a cosmetic, a raw material or a medicine with a hair-blacking effect.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a beclomethasone propionate suspension for inhalation and a preparation method of the beclomethasone propionate suspension. The preparation method of the beclomethasone propionate suspension for inhalation mainly comprises the following steps: S1, preparing sterile beclomethasone propionate particles by using a supercritical fluid rapid expansion method, and collecting the sterile beclomethasone propionate particles into a sterile glass beaker; s2, adding sterile water in which a surfactant is dissolved, and dispersing by ultrasonic dispersion equipment; s3, preparing an auxiliary material solution of beclomethasone propionate, and filtering by using a sterile filter membrane; s4, mixing the solution in the step S2 and the solution in the step S3, and dispersing by using ultrasonic dispersion equipment; and step S5, filling and sealing into an ampoule bottle to prepare the beclomethasone propionate suspension for inhalation. The beclomethasone propionate suspension for inhalation is more uniform in particle size distribution and good in stability, the preparation process is carried out under relatively mild conditions, and the technological operation is simple and convenient.

The invention belongs to the technical field of fruit and vegetable disease detection and identification, and relates to a kiwi fruit soft rot volatile biomarker identification method and a soft rot identification method, the identification method comprises the following steps: S1, selecting kiwi fruit for disinfection and sterilization; s2, inoculating pathogenic fungi: dividing the kiwi fruits into a healthy control group and a germ inoculating group; sterile water is inoculated to the healthy control group, and pathogenic fungi causing soft rot are inoculated to the germ inoculation group; healthy control samples and germ samples are randomly extracted every day during storage; s3, using a GC-IMS analyzer for the extracted sample to obtain a three-dimensional spectrogram of the volatile components; and S4, analyzing and identifying the volatile biomarker through VOCal software. According to the method, the volatile biomarker of the soft rot of the kiwi fruit is identified, and then whether the soft rot of the kiwi fruit is generated or not is identified through the identified volatile biomarker, so that the method has the characteristics of rapidness, accuracy, sensitivity and no damage, and monitoring and early warning of the soft rot of the kiwi fruit can be realized.

The invention discloses a method for inducing a regenerated plant based on a small spike stalk of bamboo-stalk, and relates to the technical field of regenerated plants.The method comprises the steps that the small spike of flowering bamboo-stalk is taken, disinfected, washed with sterile water and placed on sterile filter paper to absorb moisture, and dissected to obtain the small spike stalk as an explant; the small spike stalks are inoculated into a callus induction culture medium and cultured for 1-2 weeks under the dark condition, callus is formed through induction, and the induction culture medium comprises an MS culture medium, 2, 4-D, KT, ferulic acid, aspartic acid, Yb, an amino acid mixture and cane sugar; the callus is inoculated into a differential culture medium and cultured under the illumination condition, adventitious buds are differentiated, and the rooting culture medium comprises a 1 / 2 MS culture medium, IBA, NAA, ferric citrate, beta-salicylic acid and cane sugar. According to the method disclosed by the invention, a callus induction system starting from reproductive tissues of the bamboo-stalk bamboo is established, regenerated plants are successfully obtained, and a technical means is provided for rapid propagation of bamboo-stalk bamboo seedlings.

The invention relates to the technical field of probiotic preparations, in particular to an Ackerman bacterium probiotic preparation and application of the Ackerman bacterium probiotic preparation in prevention of bacterial pneumonia of chicks, the Ackerman bacterium probiotic preparation is prepared from the following raw materials in parts by weight: 40-60 parts of compound probiotics, 10-25 parts of prebiotics, 10-18 parts of a carrier and 8-15 parts of a freeze-drying protective additive, the preparation method of the Ackerman bacteria probiotic preparation comprises the following steps: adding the prebiotics and the freeze-drying protective agent into sterile water, and uniformly stirring to prepare a mixed solution; and fully mixing the composite probiotics with the mixed solution and the carrier, performing freeze drying, controlling the water content to be 3-5% after drying, and performing granulation to obtain the Ackerman bacteria probiotic preparation. The Ackerman bacterium probiotic preparation can significantly reduce the morbidity and mortality of bacterial pneumonia of chicks, and provides a new efficient and green disease prevention and control strategy for the poultry breeding industry.

The invention belongs to the technical field of medicines, and provides a preparation technology of a sterile injection of rerugolix, which comprises the following steps: redissolving freeze-dried powder consisting of raw material medicines and auxiliary materials by using sterile water for injection, slowly releasing the rerugolix within 2-8 weeks, maintaining the dynamic balance of blood concentration, and preparing the sterile injection of the rerugolix by using the freeze-dried powder to prepare the sterile injection of the rerugolix. Release of gonadotropin is effectively controlled, and the pharmaceutical composition is used for treating indications such as prostatic cancer, uterine fibroma and endometriosis.

The invention belongs to the technical field of sterile hydrogel patches, and particularly relates to a high-adhesion sterile hydrogel patch and a preparation method thereof. According to the preparation method of the high-adhesion sterile hydrogel patch, the high-adhesion sterile hydrogel patch is of a three-layer structure, the middle is a gel layer, the upper layer is a PE type release film, and the lower layer is a PE plastic sheet. According to the high-adhesion sterile hydrogel patch and the preparation method thereof, the formula of the high-adhesion sterile hydrogel patch is adjusted, a double-crosslinking system is utilized, the strength and toughness of hydrogel are guaranteed, meanwhile, the high-adhesion sterile hydrogel patch has high viscosity, low water loss rate and low swelling performance, the product can be repeatedly washed and worn, and in addition, the application range of the high-adhesion sterile hydrogel patch is widened. The cold compress analgesic effect is good; the invention further provides a preparation method, the preparation process is simple, and the whole process is non-toxic and harmless.

The invention discloses functional roxburgh rose fermented rice wine and a preparation method thereof. The preparation method comprises the following steps: activating a fermentation strain and carrying out enlarged culture; preparing sterile water; soaking and steaming organic glutinous rice; saccharifying the organic glutinous rice; inoculating a fermentation strain; the addition amount of roxburgh rose; and fermenting the obtained fermentation raw material at a constant temperature of 29 + / -1 DEG C for 20 days. According to the method, non-saccharomyces cerevisiae and saccharomyces cerevisiae are adopted for mixed bacteria sequential fermentation, comprehensive release and activity enhancement of nutritional ingredients in the roxburgh rose and the rice wine can be effectively promoted, the nutritional ingredients are more easily absorbed and utilized by a human body, and therefore the health care value of the roxburgh rose rice wine is greatly improved. The roxburgh rose rice wine is fragrant, tasty and refreshing, low in alcoholic strength and unique in flavor, and contains various functional active substances such as roxburgh rose polysaccharide, vitamin C, flavone, phenolic substances and SOD (superoxide dismutase).

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention relates to the technical field of ganoderma lucidum fermentation processes, and particularly discloses an aspergillus cristatus ganoderma lucidum fermentation process which comprises the following steps: S1, transferring a fungus block into a 5% NaCl + MYA solid culture medium in a super clean bench, culturing for 7 days under the condition of constant temperature and constant humidity of 28 DEG C, and repeating the process twice to activate a strain; inoculating the activated strain to a 5% NaCl + MYA plate culture medium, and culturing for 7 days under the condition of constant temperature and constant humidity of 28 DEG C; the method comprises the following steps: adding a proper amount of sterile water into an aspergillus cristatus flat plate cultured for 7 days, scraping spores, adding 1mL of spore liquid into a centrifugal tube filled with glass beads, shaking to break wall of ascospores, counting by using a blood cell counting plate, and diluting into spore suspension with corresponding concentration; s2, weighing a proper amount of ganoderma lucidum blocks, putting the ganoderma lucidum blocks into a fermentation bottle, adding distilled water, sterilizing for 20 minutes at 121 DEG C, cooling to room temperature, inoculating a proper amount of spore suspension, and culturing for 10 days at constant temperature and humidity at 28 DEG C; the aspergillus cristatus fermented ganoderma lucidum sporocarp obtained by the method is high in biological activity, and the bitter taste of ganoderma lucidum is obviously improved.

The invention discloses a microbiological separation device for clinical microbiological examination, and relates to the technical field of microbiological examination. The device comprises a bin body, a sealing door is arranged at the front end of the bin body, and a stirring type separation mechanism is arranged, so that a microorganism sample and sterile water are fed into a discharging hopper through a feeding pipe and then enter a centrifugal bin through the discharging hopper, and through cooperation of a long rod, a telescopic spring, an arc-shaped clamping block, a clamping groove and other assemblies, the stirring type separation mechanism is arranged; when the motor is started to drive the rotating shaft to rotate anticlockwise, the rotating shaft drives the stirring head to stir a microorganism sample and sterile water in the centrifugal bin, so that the microorganism sample and the sterile water are fully mixed, and the centrifugal bin is kept in an original position, and then when the motor is started to drive the rotating shaft to rotate clockwise, the centrifugal bin is driven to rotate synchronously; microorganisms are separated into the separation bin through centrifugal force generated by rapid rotation of the centrifugal bin in cooperation with the connecting pipe and the filter screen in the connecting pipe, so that the separation work is completed, the stirring time is controllable, and the whole device is more convenient to use.

The invention relates to the technical field of fermented bean products, in particular to fermented bean curd rich in nattokinase and a preparation method of the fermented bean curd. Comprising the following steps: carrying out strain activation and enlarged culture on bacillus natto, centrifuging, and removing supernatant to obtain a bacillus natto precipitate; uniformly mixing mucor and bacillus natto precipitates with sterile water in proportion to prepare a strain suspension; preparing a bean curd blank, spraying the strain suspension, and carrying out primary fermentation to obtain a bean curd blank; spreading table salt on the surface of the bean curd blank to pickle the bean curd blank to obtain a salted blank; preparing a soup base, inoculating the bacillus natto precipitate, fermenting, and preparing a fermented bean curd soup base; and putting the salt blank and the fermented bean curd soup base into a container, sealing, and carrying out post-fermentation under the condition of constant temperature, so as to obtain the fermented bean curd rich in nattokinase. The method has the advantages that the biological activity of the nattokinase in a salt environment is improved by a method of reducing the use amount of the salt and performing multiple fermentation on the bacillus natto; and a fermentation microbial enzyme system is enriched.

The preparation method comprises the following steps: uniformly mixing dry actinidia arguta powder, white granulated sugar and sterile water according to a certain ratio to prepare a fermentation stock solution, then adding bifidobacterium, wine yeast and streptococcus thermophilus, and carrying out combined fermentation at the fermentation temperature of 30-35 DEG C, so as to obtain the actinidia arguta enzyme. The inoculum sizes of the bifidobacterium, the wine saccharomycetes and the streptococcus thermophilus are all 1%-2%, the inoculum sizes are all the volume ratio of a strain seed solution to a fermentation stock solution, and sterilization is performed after fermentation is finished to obtain the actinidia arguta enzyme. The raw materials for preparing the actinidia arguta enzyme are simple and safe, the fermentation process is controllable, the three specific strains including the bifidobacterium, the wine yeast and the streptococcus thermophilus are adopted for combined fermentation, the finished product is excellent in sense, high in antioxidant activity and high in total phenol content, and a novel method for preparing the actinidia arguta enzyme is provided.

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.