377 results about "Sterile water" patented technology

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

The invention discloses a growth regulator for promoting colonization of plant rhizosphere microorganisms and application of the growth regulator to crops, a preparation method of the growth regulator comprises the following steps: S1, dropwise adding an aqueous solution of modified chitosan and a compound bacteria solution into a saturated boric acid solution of 1-2% calcium chloride, crosslinking and curing for 3-4 hours, washing with sterile water for 3-5 times, and drying to obtain the growth regulator for promoting colonization of the plant rhizosphere microorganisms; immobilized microorganisms are obtained; s2, sequentially adding nanocellulose crystals, sodium polyaspartate and organic fertilizer into the enteromorpha extracting solution, uniformly stirring and mixing, adding immobilized microorganisms, uniformly mixing, granulating, coating with modified chitosan, and drying to obtain the growth regulator. Through step-by-step design of microorganism immobilization, nutrition compounding and chitosan coating, function integration of microorganism protection, nutrition release and environmental response is achieved, the colonization efficiency of rhizosphere microorganisms is remarkably improved, the soil microenvironment is improved, and finally comprehensive improvement of plant growth and stress resistance is achieved; and the method has relatively high social use value and application prospect.

The invention relates to the field of microorganism sampling detection, in particular to escherichia coli sampling detection equipment for mouse experiments. The technical problem to be solved is that when mouse intestinal escherichia coli is sampled and detected, attention needs to be highly concentrated during manual operation, physical and mental exhaustion is easily caused, and the intestinal tract is damaged by slight carelessness. In addition, in the prior art, adipose tissue existing on the outer surface of the intestinal tract cannot be removed, and therefore the sampling detection result is affected. According to the technical scheme, the escherichia coli sampling and detecting equipment for the mouse experiment comprises a sampling box, a fixing assembly and the like; and a fixing assembly is mounted in the sampling box. According to the device, the left end and the right end of the intestinal tract of a mouse are fixed through the first clamping block and the second clamping block respectively, sterile water is injected into the intestinal tract of the mouse through an injection pipe for flushing, and cleaning of the intestinal tract of the mouse is completed, so that the pressure of manual operation is relieved, and meanwhile accidental damage to the intestinal tract of the mouse caused by manual operation is reduced.

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention discloses a preparation method of monensin fermentation liquor, relates to the technical field of microbial fermentation, and aims to solve the problems of low titer and long period of the existing process. According to the method, streptomyces cinnamomi is taken as a strain, a culture medium with a specific formula is adopted, and fermentation is performed in three stages: in the first stage (0-30-50 hours), pH is not controlled; in the second stage, a phosphoric acid and compound oil mixed solution (accounting for 1-3% of the fermentation solution, and the compound oil contains 20-30% of peanut oil, 20-30% of corn oil and the like) is supplemented at a time; in the third stage, the pH is controlled to be 6.2-6.3, and soybean oil and sterile water are supplemented according to needs. The fermentation adopts a secondary process, the temperature is controlled at 30-33 DEG C, the dissolved oxygen is more than or equal to 35% and the like, the total duration is less than or equal to 320 hours, and the titer is more than or equal to 55000 mu g / ml. By optimizing the culture medium and precisely regulating and controlling, the element production capacity is improved, the method is suitable for industrial production of tanks of 50 L to 100 m < 3 >, the efficiency is improved, and the cost is reduced.

The invention discloses a cryoprotectant which comprises glycerol, skimmed milk powder, mannitol, trehalose, vitamin C, fructo-oligosaccharide, inulin and sterile water. By optimizing the components of the cryoprotectant, the activity of the coprophilous fungi can be effectively protected, the survival rate of the intestinal coprophilous fungi in the freezing process is remarkably increased, all the used components are food-grade edible components and have a wide application range and economic effects, the invention further discloses a coprophilous fungi freezing preservation method adopting the cryoprotectant, and the cryoprotectant has good application prospects. The method is simple, easy to operate and suitable for large-scale production and application.

The invention relates to the technical field of probiotic preparations, in particular to an Ackerman bacterium probiotic preparation and application of the Ackerman bacterium probiotic preparation in prevention of bacterial pneumonia of chicks, the Ackerman bacterium probiotic preparation is prepared from the following raw materials in parts by weight: 40-60 parts of compound probiotics, 10-25 parts of prebiotics, 10-18 parts of a carrier and 8-15 parts of a freeze-drying protective additive, the preparation method of the Ackerman bacteria probiotic preparation comprises the following steps: adding the prebiotics and the freeze-drying protective agent into sterile water, and uniformly stirring to prepare a mixed solution; and fully mixing the composite probiotics with the mixed solution and the carrier, performing freeze drying, controlling the water content to be 3-5% after drying, and performing granulation to obtain the Ackerman bacteria probiotic preparation. The Ackerman bacterium probiotic preparation can significantly reduce the morbidity and mortality of bacterial pneumonia of chicks, and provides a new efficient and green disease prevention and control strategy for the poultry breeding industry.

The invention belongs to the technical field of medicines, and provides a preparation technology of a sterile injection of rerugolix, which comprises the following steps: redissolving freeze-dried powder consisting of raw material medicines and auxiliary materials by using sterile water for injection, slowly releasing the rerugolix within 2-8 weeks, maintaining the dynamic balance of blood concentration, and preparing the sterile injection of the rerugolix by using the freeze-dried powder to prepare the sterile injection of the rerugolix. Release of gonadotropin is effectively controlled, and the pharmaceutical composition is used for treating indications such as prostatic cancer, uterine fibroma and endometriosis.

The invention belongs to the technical field of sterile hydrogel patches, and particularly relates to a high-adhesion sterile hydrogel patch and a preparation method thereof. According to the preparation method of the high-adhesion sterile hydrogel patch, the high-adhesion sterile hydrogel patch is of a three-layer structure, the middle is a gel layer, the upper layer is a PE type release film, and the lower layer is a PE plastic sheet. According to the high-adhesion sterile hydrogel patch and the preparation method thereof, the formula of the high-adhesion sterile hydrogel patch is adjusted, a double-crosslinking system is utilized, the strength and toughness of hydrogel are guaranteed, meanwhile, the high-adhesion sterile hydrogel patch has high viscosity, low water loss rate and low swelling performance, the product can be repeatedly washed and worn, and in addition, the application range of the high-adhesion sterile hydrogel patch is widened. The cold compress analgesic effect is good; the invention further provides a preparation method, the preparation process is simple, and the whole process is non-toxic and harmless.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention relates to the technical field of fermented bean products, in particular to fermented bean curd rich in nattokinase and a preparation method of the fermented bean curd. Comprising the following steps: carrying out strain activation and enlarged culture on bacillus natto, centrifuging, and removing supernatant to obtain a bacillus natto precipitate; uniformly mixing mucor and bacillus natto precipitates with sterile water in proportion to prepare a strain suspension; preparing a bean curd blank, spraying the strain suspension, and carrying out primary fermentation to obtain a bean curd blank; spreading table salt on the surface of the bean curd blank to pickle the bean curd blank to obtain a salted blank; preparing a soup base, inoculating the bacillus natto precipitate, fermenting, and preparing a fermented bean curd soup base; and putting the salt blank and the fermented bean curd soup base into a container, sealing, and carrying out post-fermentation under the condition of constant temperature, so as to obtain the fermented bean curd rich in nattokinase. The method has the advantages that the biological activity of the nattokinase in a salt environment is improved by a method of reducing the use amount of the salt and performing multiple fermentation on the bacillus natto; and a fermentation microbial enzyme system is enriched.

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.

The invention relates to a preparation method of compound microorganism bacterium soil, microorganisms in the compound microorganism bacterium soil comprise bacillus subtilis YCBA0319 with the preservation number of CGMCC No.33006 and trichoderma longibrachiatum YCFA0522 with the preservation number of CGMCC No.41655. The preparation method comprises the following steps: selecting relatively clean clay or loam, and removing stones and plant residues; mixing the fine soil, the soybean meal and the cottonseed cake, stirring by using a double-shaft paddle type mixer, and spraying sterile water to adjust the water content; sub-packaging into polypropylene woven bags, sterilizing, and cooling for later use; preparing a seed solution of the bacillus subtilis YCBA0319; preparing a spore suspension of the trichoderma longibrachiatum YCFA0522; the bacillus subtilis is 2.5 * 10 < 9 > CFU / g, and the trichoderma longibrachiatum is 5.0 * 10 < 9 > CFU / g. According to the technical scheme, a granular structure is formed with soil in the fermentation process, so that an appropriate living microenvironment is provided for microorganisms, effective microbial communities are protected from being influenced by external adverse conditions, and the activity and function exertion of the effective microbial communities after field application are ensured.

The invention discloses a preparation method and application of a biochar immobilized microbial agent, and relates to the technical field of immobilized microbial treatment.The preparation method mainly comprises the following steps that a bacterial strain serratia marcescens QZB-1 is activated and then centrifugally collected, and a bacterial suspension is prepared with sterile water; fully and uniformly mixing the sterilized biochar with the bacterial suspension, and oscillating for 24 hours; adding sodium alginate into the mixture, uniformly mixing, slowly dripping the mixture into a 3% calcium chloride solution by using a rubber head suction tube, and standing and cross-linking for 12 hours to prepare a biochar immobilized microbial agent; and finally, flushing away the calcium chloride solution remained on the surface of the biochar immobilized microbial agent by using sterilized normal saline, and storing in the sterilized normal saline for later use. The biochar immobilized microbial agent disclosed by the invention has relatively high removal capacity on Mn (II), and has a very good application prospect in manganese pollution treatment.

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The invention belongs to the technical field of microbiological detection, and particularly relates to a rapid escherichia coli test box which comprises a detection box, a sterile box, a multi-stage feeding device and a first motor, the first motor controls the working stroke of the multi-stage feeding device, and then the dosage of sterile water added into the sterile box is controlled; the farther the first motor is, the more sterile liquid is added into the sterile box, the smaller the dilution degree is, and the closer the first motor is, the less sterile liquid is added into the sterile box, and the larger the dilution degree is, so that the three sterile boxes are sequentially placed, and three diluents with different dilution degrees are obtained; the method does not need to manually separately use a sterilization suction tube to suck sterile water of different doses and add the sterile water into diluents of different diluents to obtain the diluents of different diluents, so that the detection time is greatly saved, and the detection efficiency is improved.

The invention provides a tobacco plant inspection and treatment device, and belongs to the field of tobacco plant treatment device.The tobacco plant inspection and treatment device comprises a material placing table, a cutting assembly is arranged on one side of the material placing table, and the material placing table is connected with a material table driving assembly used for driving the material placing table to move in the direction close to the cutting assembly; the cutting assembly is used for cutting tobacco plant samples on the discharging table, one side of the cutting assembly is provided with an alcohol rinsing pool, one side of the alcohol rinsing pool is provided with a cleaning conveying net, the upper side of the cleaning conveying net is provided with a plurality of nozzles, one side of the cleaning conveying net is provided with a mercury rinsing pool, and the mercury rinsing pool is provided with a mercury discharge port. And a plurality of sterile water rinsing pools are arranged on one side of the mercury rinsing pool. The treatment efficiency of the tobacco plant sample required by the experiment is improved.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a rapid tissue propagation method for radix ranunculi ternati. The rapid propagation method for the radix ranunculi ternati tissue comprises the following steps: (1) seed selection and disinfection: selecting vigorous seeds, cleaning the seeds, soaking the seeds with mercury bichloride, disinfecting the seeds with alcohol, and finally washing the seeds with sterile water; (2) preparing a primary induction culture medium: preparing the primary induction culture medium; (3) seed induction and proliferation: inoculating the disinfected seeds on a primary induction culture medium, and culturing; after 4-6 weeks of culture, the seeds germinate into plantlets; transferring the plantlets to a subculture induction medium for multiplication culture, and subculturing once every 3-4 weeks; (4) rooting culture: transferring the proliferation seedlings to a rooting culture medium, and culturing for 2-3 weeks until the adventitious buds can root to form complete plants; and (5) seedling hardening and transplanting. The method can improve the rooting rate and shorten the propagation time at the same time.

The invention belongs to the technical field of strain identification and drug sensitivity detection, and discloses a strain identification and drug sensitivity detection kit and a preparation method and application thereof, the kit comprises a drug sensitivity disc, a pre-enrichment chromogenic culture medium, a chromogenic culture medium, sterile water and a diluent; the drug sensitive disc body comprises an enterobacteriaceae area, an enterococcus area, a staphylococcus area, a pseudomonas aeruginosa area and an acinetobacter baumannii area; the enterobacteriaceae area is provided with enterobacteriaceae identification culture medium micropores and drug micropores, the enterococcus area is provided with enterococcus identification culture medium micropores and drug micropores, the staphylococcus area is provided with staphylococcus identification culture medium micropores and drug micropores, and the pseudomonas aeruginosa area is provided with pseudomonas aeruginosa identification culture medium micropores and drug micropores. An acinetobacter baumannii identification culture medium micropore and a medicine micropore are matched in the acinetobacter baumannii area; the problems that an existing drug sensitivity test mode is complex in operation, high in cost and low in accuracy of identification and detection results are solved.

The invention relates to the technical field of medical equipment, and provides a prostate steam ablation control method and device and a storage medium, and the control method comprises the steps: controlling a water supply part to supply sterile water to a steam generation pipe according to a first water pushing speed, and controlling a heating assembly to heat the steam generation pipe at a first power, sterile water in the steam generation pipe is preheated to a target temperature; controlling the water supply piece to supply sterile water to the steam generation pipe according to a second water pushing speed, controlling the heating assembly to heat the steam generation pipe at a second power, heating the sterile water at the target temperature into ablation steam, and applying the ablation steam to a target ablation area by the steam ablation needle; and controlling the water supply piece to supply sterile water to the steam generation pipe according to the third water pushing speed, and controlling the heating assembly to heat the steam generation pipe at the first power. During steam ablation treatment, the stability of output ablation steam heat can be ensured, steam is prevented from burning a human body, steam holes of the steam ablation needle are prevented from being blocked, and the steam ablation treatment effect is ensured.

The invention discloses a production method and application of a fulvic acid culture medium with the content reaching 95%. The culture medium takes weathered coal as a raw material and comprises 30-70 g of humic acid, 4-30 g of fulvic acid, 2-20 g of ulmic acid and 2-20 g of humic acid. The ash content is 4-15%, the ratio of C to N is (25-30): 1, the organic matter is 40-80%, and the N.P.K content is 3-4%; the formula comprises 40-70% of humic acid, 6-30% of fulvic acid, 3-15% of ulmic acid and 3-15% of black humic acid. During production, 800-1000 g of raw materials and 400-500 g of sterile water are stirred, 1-2 g of stock seeds are added, the pH value is adjusted to be 6-7, the temperature of an incubator is controlled to be 48-60 DEG C, the humidity is controlled to be 75-85 DEG C, a proper amount of oxygen is added, and shading fermentation is conducted for 48-72 h. The obtained enzyme bacteria contain various organic acids, various amino acids, various oxygen-containing functional groups, various life elements and the like, the viability of beneficial bacteria reaches 90-260 billion / g and is far beyond the standard of 0.2 billion / g of the national standard, meanwhile, the bacteria can also be used for producing third-level bacterial manure which is suitable for field and gardening cultivation, and the effect is extremely good. After application, vicious circle of chemical fertilizer and pesticide can be converted into ecological virtuous circle, and technical progress is highlighted.

The present invention discloses a method for improving the quality of reconstituted tobacco, comprising the following steps: picking a single colony of Pseudomonas paraflavonoids ZY-03 with a Tibetan number of CGMCC NO.24422 and inoculating it into a liquid culture medium to obtain a seed liquid; centrifuging the seed liquid to remove the supernatant, adding sterile water to redissolve it, preparing a bacterial suspension, adding a protective agent, vacuum drying, and preparing bacterial powder; adding bacterial powder to a papermaking pulp, and then fermenting it to form a substrate; adding bacterial powder to a tobacco extract, and then concentrating it after fermentation to form a coating liquid; and drying it after coating. This application combines the process of reconstituted tobacco itself, uses Pseudomonas paraflavonoids ZY-03 to prepare a bacterial agent dry powder, and adds the bacterial powder to the papermaking pulp and tobacco extract respectively for fermentation, efficiently degrading lignin. At the same time, the rich enzymes produced by the strain have a certain flavoring effect, are sterilized in the drying stage, reduce the lignin in the reconstituted tobacco substrate, and increase the aroma of the coating liquid to improve the quality of reconstituted tobacco.

The invention relates to the technical field of environmental monitoring, in particular to a method for measuring soil or water salinity by using microorganisms and application. In a salt environment, the growth rate of the selected microorganisms is related to the salinity; when the salinity is enhanced, the growth of microorganisms is inhibited; according to the method, the salinity of the environment is judged by monitoring microorganisms OD600 in real time; the method comprises the following steps: selecting Nocardia listeria BFY2-1 as a strain to be tested as a microorganism, and selecting Nocardia listeria BFY2-1 as a strain to be tested; a to-be-tested water body or soil 1: 5 suspension; adjusting the pH value to 6-9, and taking 20 ML; adding 10 ML of a three-time culture medium, inoculating a test strain, and comparing with sterile water; carrying out shake cultivation at 28 DEG C, wherein the rotating speed is 200 rpm; after 48 hours, measuring the absorbance at the wavelength of 600 nm by using a spectrophotometer; calculating the salt content according to the absorbance, and converting the initial salt content according to the dilution.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention provides a method for breeding curcuma aromatica through a liquid culture medium and application of the curcuma aromatica, and belongs to the technical field of plant culture.The method comprises the following steps that 1, curcuma aromatica seeds are taken, cleaned and then sequentially disinfected through ethyl alcohol, soaked in a sodium hypochlorite solution, soaked in sterile water and flushed, and disinfected seeds are obtained; (2) inoculating the disinfected seeds to a liquid culture medium, and performing germination culture on the sterile seeds on the liquid culture medium to obtain sterile tissue culture seedlings; and (3) cutting the sterile tissue culture seedlings into stem sections of 2-3cm, inoculating the stem sections with at least one pair of axillary buds to the liquid culture medium with the same composition in the step (2), and carrying out subculture on the tissue culture seedlings. The method provided by the invention has a good tissue culture effect on radix curcumae, can simultaneously realize excellent culture effects in four aspects of germination rate of seeds, average germination rate of tissue culture seedlings, growth height and differentiation rate, and has extremely high research value and application prospect.

The invention discloses an anaerobic denitrifying bacterial agent as well as a preparation method and application thereof, and belongs to the technical field of water environment treatment. The anaerobic denitrifying bacterium agent is prepared from the following components in parts by weight: 15 to 30 parts of sterile water, 15 to 60 parts of normal saline, 5 to 10 parts of micro-capsule embedded anaerobic denitrifying bacteria, 5 to 20 parts of a porous composite modified carrier, 10 to 30 parts of a carbon source, 4 to 7 parts of nutrient elements, 2.5 to 4 parts of a buffering agent and 1 to 5 parts of a stabilizer, the micro-capsule embedded anaerobic denitrifying bacteria are obtained by embedding anaerobic denitrifying bacteria with an alginic acid gel solution and a calcium chloride solution; the porous composite modified carrier is obtained by crosslinking modified nano iron oxide, modified nano titanium dioxide, a functional polymer solution and glutaraldehyde. The anaerobic denitrifying bacterial agent provided by the invention can effectively improve the efficiency of treating nitrogen pollution of a water body.

This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.

The invention relates to the technical field of medicines, in particular to novel application of galactooligosaccharide. The novel use of galactooligosaccharide inhibits the formation of gallstone. Main reagents and materials are galactooligosaccharide; a mouse calculus model feed; the experimental animals are derived from animal varieties and strains: mice, C57BL / 6J; sex: male; week age: 4 week age; the body weight is 16-20 g; the experimental environment is SPF environment feeding, and the feeding condition is that the room temperature is 20-26 DEG C; the relative humidity is 40-70%; automatic illumination is achieved, and light and shade are alternated for 12 h; the drinking water is RO membrane reverse osmosis sterile water; the feeding density is defaulted to be 4 per cage, and the padding is replaced once a week; the experimental process comprises the following steps: starting an experiment after experimental feeding of all mice for one week, and randomly dividing 36 5-week male C57BL / 6J mice into three groups, namely 12 mice in each group. The invention provides a new application of galactooligosaccharide, which can inhibit the formation of gallstone and provides a new thought for preventing the occurrence of the gallstone.

The invention belongs to the field of biological agents, and discloses preparation and application of an anaerobe petroleum hydrocarbon degrading agent. The preparation method of the anaerobe petroleum hydrocarbon degrading bacterial agent comprises the following steps: mixing a strain, an activating agent and sterile water, stirring and activating to obtain an activated bacterial solution; adding the activated bacterial liquid and bran into the nutrient solution, and then fermenting; and centrifuging the fermented bacterial suspension, collecting the precipitate, mixing the precipitate with the protective agent 1, spraying the protective agent 2 onto the surface of the mixture of the precipitate and the protective agent 1, and drying to obtain the anaerobe petroleum hydrocarbon degrading bacterial agent. The anaerobe petroleum hydrocarbon degrading bacterial agent can effectively degrade petroleum hydrocarbon wastewater, especially cutting fluid wastewater, wherein macromolecular and long-chain hydrocarbon substances can be anaerobically degraded.

The present application relates to the technical field of medical devices, and in particular to a control method of a steam ablation device and the steam ablation device. The method comprises: unfolding a camera assembly from a folded state to an unfolded state, so that the lens of the camera faces the needle cylinder slot; installing a needle cylinder preloaded with sterile water into the needle cylinder slot, so that the needle cylinder abuts against the push rod of the push rod assembly; the positioner sends a position signal in response to the abutting pressure of the needle cylinder; based on the position signal, the rotating member is controlled to rotate the needle cylinder to a first shooting angle, and a first image is collected by the camera; the rotating member is controlled to rotate the needle cylinder to a second shooting angle, and a second image is collected by the camera; based on the first image and the second image, bubble detection is performed to determine whether harmful bubbles exist in the needle cylinder; if no harmful bubbles are detected, the push rod assembly is controlled to push the sterile water in the needle cylinder to an external device. The method collects two images in different directions for bubble detection, thereby improving the comprehensiveness of bubble detection.