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178 results about "Sterile water" patented technology

Sterile water is water that has no microorganisms living within it. As it cannot transfer pathogens, it is used mainly in the medical setting. Sterile water, though sometimes distilled, is not the same thing as distilled water.

Fermentation process of citric acid residues

PendingCN121465153AAnimal feeding stuffSporelingSpore Count
The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.
Owner:HEFEI FISUGARPEPTIDE BIOTECH CO LTD

Preparation method of monensin fermentation liquor

The invention discloses a preparation method of monensin fermentation liquor, relates to the technical field of microbial fermentation, and aims to solve the problems of low titer and long period of the existing process. According to the method, streptomyces cinnamomi is taken as a strain, a culture medium with a specific formula is adopted, and fermentation is performed in three stages: in the first stage (0-30-50 hours), pH is not controlled; in the second stage, a phosphoric acid and compound oil mixed solution (accounting for 1-3% of the fermentation solution, and the compound oil contains 20-30% of peanut oil, 20-30% of corn oil and the like) is supplemented at a time; in the third stage, the pH is controlled to be 6.2-6.3, and soybean oil and sterile water are supplemented according to needs. The fermentation adopts a secondary process, the temperature is controlled at 30-33 DEG C, the dissolved oxygen is more than or equal to 35% and the like, the total duration is less than or equal to 320 hours, and the titer is more than or equal to 55000 mu g / ml. By optimizing the culture medium and precisely regulating and controlling, the element production capacity is improved, the method is suitable for industrial production of tanks of 50 L to 100 m < 3 >, the efficiency is improved, and the cost is reduced.
Owner:SHANGHAI MOXI BIOLOGICAL TECH CO LTD

Method for improving photosynthetic characteristics and biomass of equisetum arvense and equisetum arvense cultivation method

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.
Owner:CHONGQING ACAD OF ANIMAL SCI

Rapid test box for escherichia coli

The invention belongs to the technical field of microbiological detection, and particularly relates to a rapid escherichia coli test box which comprises a detection box, a sterile box, a multi-stage feeding device and a first motor, the first motor controls the working stroke of the multi-stage feeding device, and then the dosage of sterile water added into the sterile box is controlled; the farther the first motor is, the more sterile liquid is added into the sterile box, the smaller the dilution degree is, and the closer the first motor is, the less sterile liquid is added into the sterile box, and the larger the dilution degree is, so that the three sterile boxes are sequentially placed, and three diluents with different dilution degrees are obtained; the method does not need to manually separately use a sterilization suction tube to suck sterile water of different doses and add the sterile water into diluents of different diluents to obtain the diluents of different diluents, so that the detection time is greatly saved, and the detection efficiency is improved.
Owner:徐文龙

Rapid propagation method for radix ranunculi ternati tissue

PendingCN121795322Aprevent excessive growthAvoid callus overproliferation problemsPlant tissue cultureHorticulture methodsSterile waterPlantlet
The invention belongs to the technical field of plant tissue culture, and particularly relates to a rapid tissue propagation method for radix ranunculi ternati. The rapid propagation method for the radix ranunculi ternati tissue comprises the following steps: (1) seed selection and disinfection: selecting vigorous seeds, cleaning the seeds, soaking the seeds with mercury bichloride, disinfecting the seeds with alcohol, and finally washing the seeds with sterile water; (2) preparing a primary induction culture medium: preparing the primary induction culture medium; (3) seed induction and proliferation: inoculating the disinfected seeds on a primary induction culture medium, and culturing; after 4-6 weeks of culture, the seeds germinate into plantlets; transferring the plantlets to a subculture induction medium for multiplication culture, and subculturing once every 3-4 weeks; (4) rooting culture: transferring the proliferation seedlings to a rooting culture medium, and culturing for 2-3 weeks until the adventitious buds can root to form complete plants; and (5) seedling hardening and transplanting. The method can improve the rooting rate and shorten the propagation time at the same time.
Owner:NANJING AGRICULTURAL UNIVERSITY

Strain identification and drug sensitivity detection kit as well as preparation method and application thereof

The invention belongs to the technical field of strain identification and drug sensitivity detection, and discloses a strain identification and drug sensitivity detection kit and a preparation method and application thereof, the kit comprises a drug sensitivity disc, a pre-enrichment chromogenic culture medium, a chromogenic culture medium, sterile water and a diluent; the drug sensitive disc body comprises an enterobacteriaceae area, an enterococcus area, a staphylococcus area, a pseudomonas aeruginosa area and an acinetobacter baumannii area; the enterobacteriaceae area is provided with enterobacteriaceae identification culture medium micropores and drug micropores, the enterococcus area is provided with enterococcus identification culture medium micropores and drug micropores, the staphylococcus area is provided with staphylococcus identification culture medium micropores and drug micropores, and the pseudomonas aeruginosa area is provided with pseudomonas aeruginosa identification culture medium micropores and drug micropores. An acinetobacter baumannii identification culture medium micropore and a medicine micropore are matched in the acinetobacter baumannii area; the problems that an existing drug sensitivity test mode is complex in operation, high in cost and low in accuracy of identification and detection results are solved.
Owner:BEIJING VETERINARY DRUG FEED MONITORING CENT +1

Prostate steam ablation control method and device and storage medium

The invention relates to the technical field of medical equipment, and provides a prostate steam ablation control method and device and a storage medium, and the control method comprises the steps: controlling a water supply part to supply sterile water to a steam generation pipe according to a first water pushing speed, and controlling a heating assembly to heat the steam generation pipe at a first power, sterile water in the steam generation pipe is preheated to a target temperature; controlling the water supply piece to supply sterile water to the steam generation pipe according to a second water pushing speed, controlling the heating assembly to heat the steam generation pipe at a second power, heating the sterile water at the target temperature into ablation steam, and applying the ablation steam to a target ablation area by the steam ablation needle; and controlling the water supply piece to supply sterile water to the steam generation pipe according to the third water pushing speed, and controlling the heating assembly to heat the steam generation pipe at the first power. During steam ablation treatment, the stability of output ablation steam heat can be ensured, steam is prevented from burning a human body, steam holes of the steam ablation needle are prevented from being blocked, and the steam ablation treatment effect is ensured.
Owner:腾云医疗(深圳)有限公司

Method for determining salinity of soil or water body by using microorganisms and application

The invention relates to the technical field of environmental monitoring, in particular to a method for measuring soil or water salinity by using microorganisms and application. In a salt environment, the growth rate of the selected microorganisms is related to the salinity; when the salinity is enhanced, the growth of microorganisms is inhibited; according to the method, the salinity of the environment is judged by monitoring microorganisms OD600 in real time; the method comprises the following steps: selecting Nocardia listeria BFY2-1 as a strain to be tested as a microorganism, and selecting Nocardia listeria BFY2-1 as a strain to be tested; a to-be-tested water body or soil 1: 5 suspension; adjusting the pH value to 6-9, and taking 20 ML; adding 10 ML of a three-time culture medium, inoculating a test strain, and comparing with sterile water; carrying out shake cultivation at 28 DEG C, wherein the rotating speed is 200 rpm; after 48 hours, measuring the absorbance at the wavelength of 600 nm by using a spectrophotometer; calculating the salt content according to the absorbance, and converting the initial salt content according to the dilution.
Owner:SHAANXI INST OF BIOLOGICAL AGRI

Streptomyces flavus C-21 as well as preparation method and application of sterile fermentation filtrate of streptomyces flavus C-21

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.
Owner:SHANXI AGRI UNIV

Anaerobic denitrifying bacterial agent as well as preparation method and application thereof

The invention discloses an anaerobic denitrifying bacterial agent as well as a preparation method and application thereof, and belongs to the technical field of water environment treatment. The anaerobic denitrifying bacterium agent is prepared from the following components in parts by weight: 15 to 30 parts of sterile water, 15 to 60 parts of normal saline, 5 to 10 parts of micro-capsule embedded anaerobic denitrifying bacteria, 5 to 20 parts of a porous composite modified carrier, 10 to 30 parts of a carbon source, 4 to 7 parts of nutrient elements, 2.5 to 4 parts of a buffering agent and 1 to 5 parts of a stabilizer, the micro-capsule embedded anaerobic denitrifying bacteria are obtained by embedding anaerobic denitrifying bacteria with an alginic acid gel solution and a calcium chloride solution; the porous composite modified carrier is obtained by crosslinking modified nano iron oxide, modified nano titanium dioxide, a functional polymer solution and glutaraldehyde. The anaerobic denitrifying bacterial agent provided by the invention can effectively improve the efficiency of treating nitrogen pollution of a water body.
Owner:CHENGDU XINBOAO OIL & GAS ENG TECH SERVICE CO LTD

A method and kit for enhancing the yield and quality of a product of gene synthesis

PendingCN122279011ANucleotideSterile water
This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.
Owner:苏州君跻基因科技有限公司

Preparation and application of anaerobe petroleum hydrocarbon degrading bacterial agent

The invention belongs to the field of biological agents, and discloses preparation and application of an anaerobe petroleum hydrocarbon degrading agent. The preparation method of the anaerobe petroleum hydrocarbon degrading bacterial agent comprises the following steps: mixing a strain, an activating agent and sterile water, stirring and activating to obtain an activated bacterial solution; adding the activated bacterial liquid and bran into the nutrient solution, and then fermenting; and centrifuging the fermented bacterial suspension, collecting the precipitate, mixing the precipitate with the protective agent 1, spraying the protective agent 2 onto the surface of the mixture of the precipitate and the protective agent 1, and drying to obtain the anaerobe petroleum hydrocarbon degrading bacterial agent. The anaerobe petroleum hydrocarbon degrading bacterial agent can effectively degrade petroleum hydrocarbon wastewater, especially cutting fluid wastewater, wherein macromolecular and long-chain hydrocarbon substances can be anaerobically degraded.
Owner:GUANGDONG VOCATIONAL & TECHNICAL COLLEGE

Control method of a vapor ablation device and vapor ablation device

PendingCN122624157AMedicineSterile water
The present application relates to the technical field of medical devices, and in particular to a control method of a steam ablation device and the steam ablation device. The method comprises: unfolding a camera assembly from a folded state to an unfolded state, so that the lens of the camera faces the needle cylinder slot; installing a needle cylinder preloaded with sterile water into the needle cylinder slot, so that the needle cylinder abuts against the push rod of the push rod assembly; the positioner sends a position signal in response to the abutting pressure of the needle cylinder; based on the position signal, the rotating member is controlled to rotate the needle cylinder to a first shooting angle, and a first image is collected by the camera; the rotating member is controlled to rotate the needle cylinder to a second shooting angle, and a second image is collected by the camera; based on the first image and the second image, bubble detection is performed to determine whether harmful bubbles exist in the needle cylinder; if no harmful bubbles are detected, the push rod assembly is controlled to push the sterile water in the needle cylinder to an external device. The method collects two images in different directions for bubble detection, thereby improving the comprehensiveness of bubble detection.
Owner:JIANGSU SHENGLONG MEDICAL TECHNOLOGY CO LTD

A high-dietary-fiber seaweed powder, and a method of preparing and using the same

PendingCN122350278ABiotechnologyFiber
This invention discloses a high-dietary-fiber seaweed powder, its preparation method, and its application, belonging to the field of food technology. The high-dietary-fiber seaweed powder is prepared by the following method: (1) seaweed is soaked in slightly acidic electrolyzed water, ultrasonically cleaned, rinsed, dried, pulverized, and sieved to obtain coarse seaweed powder; (2) the coarse seaweed powder is added to a twin-screw extruder, and deionized water and a modifier are added simultaneously at the side feed port. After rotary cutting, granulation, and cooling, modified seaweed is obtained; (3) the modified seaweed is placed in a fermentation container, mixed with sterile water containing glucose, and fermented with a compound bacterial agent; after heat preservation, cooling, pre-freezing, vacuum freeze-drying, pulverization, and sieving, the seaweed powder is obtained. The seaweed powder prepared by this invention has a high total dietary fiber content and a high soluble dietary fiber content, excellent water-holding capacity, swelling capacity, and DPPH free radical scavenging rate, and a relatively weak fishy smell.
Owner:YANTAI SANHAI BIOTECHNOLOGY CO LTD

Brewing method of lotus seed wine

The invention discloses a lotus seed wine brewing method, and belongs to the technical field of wine brewing, selenium-rich lotus seeds without lotus cores are used as a main material, the lotus cores are collected, bitter root sources are eliminated through warm water debitterizing and enzymolysis degradation, the lotus cores and rice husks are compounded and incorporated into an auxiliary material system, the rice husks adsorb residual bitter taste, selenium and flavone of the lotus cores are synchronously reserved, and the lotus seed wine is obtained. Waste utilization of the lotus seeds is facilitated, and beneficial components of the lotus seed wine are improved; in the cleaning link, normal-temperature sterile water containing food-grade oligosaccharide is adopted for low-pressure spraying, the oligosaccharide forms a protective film on the surfaces of the lotus seeds, and selenium dissolution is reduced; a low-pressure low-temperature short-time process is adopted in the cooking link, so that organic selenium and flavone are prevented from being damaged by high temperature; low-temperature sectional interception is adopted for distillation, selenium is prevented from being volatilized along with high-temperature water vapor, and active ingredients are intensively intercepted in a core wine section. According to the invention, the value of the raw materials is maximized, active ingredients are efficiently reserved, the production efficiency and the quality stability are improved, and finally, the wine has drinking experience and health-care properties of lotus seeds.
Owner:LONGYOU HEFU AGRICULTURAL DEVELOPMENT CO LTD

A portable sterile water preparation device using plasma technology

The application discloses a portable sterile water preparation device using a plasma technology and belongs to the technical field of sterile water preparation. The device comprises a shell, a splash-proof baffle, an end cover, a flow guide device, a sealing cap, a flexible material and a porous medium. The flexible material generates plasma through discharge to fill the closed shell. The porous medium has water absorption and can absorb a liquid to be treated. The plasma diffuses into the porous medium and performs sterilization treatment on the liquid to be treated. The sterile water is prepared through an indirect treatment mode. The device has the advantages of portability, simple operation and safety in the sterile water preparation process.
Owner:NANJING JULEI ELECTRONIC TECH CO LTD

Tissue culture and rapid propagation method for hemarthria procumbens

The tissue culture and rapid propagation method comprises the following steps: (1) carrying out sterile treatment and low-temperature pretreatment on a hemarthria procumbens seed; (2) carrying out germination culture on the hemarthria procumbens seeds treated in the step (1); (3) carrying out induced culture to grow calluses; (4) proliferation and differentiation culture; (5) rooting culture; wherein the low-temperature pretreatment specifically comprises the following steps: after sterile treatment, soaking the seeds in sterile water at 4 DEG C in a dark place for 12-24 hours, and carrying out low-temperature pretreatment; in the step (3), the induction culture medium is prepared from 0.5 mg / L to 0.8 mg / L of MS + 2, 4-D, 0.1 mg / L to 0.5 mg / L of 6-BA, 0.01 mg / L to 0.05 mg / L of BR and 0.5 mg / L to 1.5 mg / L of AC. According to the method, the callus induction rate is greatly increased, meanwhile, the browning rate of the callus is remarkably reduced, the callus induction rate can reach 75% or above, and the browning rate of the callus is reduced to 5% or below.
Owner:MENGCAO ECOLOGICAL ENVIRONMENT (GRP) CO LTD

A method for improving postharvest resistance to black rot and maintaining quality of sweet potato tubers

PendingCN122074546Ainhibit expansionIncreased resistance to black spot diseaseFruit and vegetables preservationBiotechnologyPesticide residue
This invention discloses a method for improving the resistance to postharvest black spot disease in sweet potato tubers and maintaining their quality, belonging to the field of postharvest preservation technology for agricultural products. The method includes: selecting undamaged sweet potato tubers for callus treatment at room temperature for 48 hours, disinfecting with 1% sodium hypochlorite, rinsing with sterile water and air-drying, then soaking them in a 9.0 mmol / L benzothiadiazole (BTH) solution (containing 0.05% Tween 80) at room temperature for 60 minutes, removing them and air-drying them to complete the treatment. This invention induces the activation of the sweet potato tuber's own defense system through BTH, increasing the activity of key enzymes in phenylpropane metabolism, chitinase and other disease-resistant enzymes, promoting the accumulation of total phenols and lignin, maintaining cell membrane integrity, inhibiting starch degradation and abnormal increase in soluble sugars. Under storage conditions of 28±1℃ and 80-85%RH, it can significantly inhibit the expansion of black spot disease lesions and extend the storage period to more than 25 days. This method has no pesticide residues, is unlikely to cause pathogen resistance, is environmentally friendly, simple to operate, and cost-controllable, and is suitable for large-scale postharvest preservation of sweet potato tubers.
Owner:ZHEJIANG FORESTRY UNIVERSITY

A biomimetic medical tissue adhesive and a preparation method thereof

ActiveCN117224731BSurgical adhesivesPolyethylene glycolOctyl cyanoacrylate
The application relates to the technical field of medical materials, and particularly discloses a kind of bionic medical tissue adhesive and a preparation method thereof.A kind of bionic medical tissue adhesive is made of raw materials containing the following weight parts: alpha-cyano octyl acrylate 30-35 parts;degradable polymer material 20-30 parts;gelatin 1-5 parts;polyethylene glycol 2-5 parts;sterile water 20-30 parts;sodium alginate 2-4 parts;antibacterial agent 8-10 parts;stabilizing protective agent 1-3 parts;mussel extract 1-3 parts;slug extract 1-3 parts;its preparation method is: polyethylene glycol, sterile water, sodium alginate, antibacterial agent, stabilizing protective agent, mussel extract and slug extract are mixed, and stirred uniformly;then alpha-cyano octyl acrylate, degradable polymer material and gelatin are added, heated to 40-60 DEG C and stirred for 20-40 min, and the bionic medical tissue adhesive can be obtained.The bionic medical tissue adhesive of the application can better handle the skin tissue changes occurring in the proliferation period, so that the prominence of the scar formed in the later period is smaller, the whole is relatively flat and smooth, and thus the scar is greatly improved.
Owner:SHANGHAI JIAXIN BIOTECHNOLOGY CO LTD

Prostate thermal steam ablation catheter and steam ablation handle

ActiveCN224441436UMedicineSterile water
This utility model relates to the field of steam ablation technology, providing a prostate thermal steam ablation catheter and steam ablation handle. The catheter includes a guide rod and a catheter tip, with the catheter tip located at the end of the guide rod. The catheter tip has a needle insertion channel and a flushing channel. The catheter tip has a first notch, a second notch, and a third notch, which surround the needle insertion channel. The sidewall of the catheter tip also has a first shielding surface and a second shielding surface. The first shielding surface guides a portion of the fluid flowing out along the first notch to flow in the opposite direction, and the second shielding surface guides a portion of the fluid flowing out along the second notch to flow in the opposite direction. This structure guides the flow of sterile water to the steam ablation center corresponding to the puncture needle, ensuring that sterile water covers three sides of the steam ablation center, forming a vortex. This allows for heat dissipation and uniform cooling of the treatment area, helping to avoid the risk of steam burns to tissue and improving the clarity of observation of the treatment area.
Owner:腾云医疗(深圳)有限公司

Laboratory ultrapure water machine with function of preparing different water quality

The utility model relates to the technical field of ultrapure water machines, and discloses a laboratory ultrapure water machine with a function of preparing different water qualities, which comprises a water purifier body and a sterile water tank, and a PP cotton filter, an activated carbon filter, a composite filter and an ultrafiltration membrane filter are arranged at the rear position in the water purifier body. The bottom end of the PP cotton filter is connected with a water outlet valve pipe of the sterile water tank through a water conveying pipe; the interior of the water purifier body is divided into a plurality of treatment cavities; through the ultra-purification treatment system, the microfiltration sterilization module, the ultra-purification treatment system, the reverse osmosis drainage pump, the circulating pump and the circulating pipe, water can be treated in sequence according to needs, so that water with different water qualities can be taken, and water flow in the water storage pipeline can be pumped and retreated; the water flow which does not pass the detection can be circulated to the water inlet for secondary or multiple times of treatment, and can be discharged until the water quality reaches the standard, so that the water treatment effect is ensured.
Owner:ZHENGZHOU JINGCHENG ENVIRONMENTAL PROTECTION TECHNOLOGY CO LTD

Screening method of selenium-rich high-yield peanut core germplasm

The present application relates to the technical field of peanut variety creation and breeding, and particularly relates to a screening method of selenium-rich high-yield peanut core germplasm. The screening method comprises the following steps: using a selenium solution to culture peanut seeds to be screened, using peanut seeds to be screened cultured by sterile water as a control, screening peanut seeds with root length higher than the control by more than 10%, and obtaining selenium-sensitive peanut seeds; after sowing the selenium-sensitive peanut seeds, performing selenium treatment, using corresponding selenium-sensitive peanut seeds cultured by sterile water as a control, after one week of selenium treatment, screening peanut seeds with plant height and aboveground dry matter weight higher than the corresponding control, and obtaining selenium-rich peanut germplasm; sowing the selenium-rich peanut germplasm, using Zhonghua No. 16 as a control, screening peanut seeds with pod yield per plant higher than the control at the mature stage, and obtaining selenium-rich high-yield peanut core germplasm. The present application has the advantages of low cost, quantification, repeatability, high efficiency and easy implementation.
Owner:CROP RES INST OF JIANGXI ACAD OF AGRI SCI

Sterilized thermostatic water bath for surgery

This invention relates to the field of medical devices and discloses a sterile constant temperature water bath for surgery, comprising a water storage tank, a water bath body, a communication and conversion mechanism, a heating mechanism, a cooling mechanism, and a sterile water storage tank. The water bath body is fixedly installed on the upper surface of the water storage tank, and the communication and conversion mechanism is provided inside the water storage tank. The heating mechanism and the cooling mechanism are fixedly installed on both sides of the upper surface of the water storage tank located on the water bath body, respectively. This invention uses a sterile water storage tank to store sterile water for rinsing. Air pressure is injected into the sterile water storage tank using an inflatable airbag and an inflation tube, squeezing the sterile water out from the inside of the suction pipe. The water is then connected to an external rinsing device through the suction pipe, achieving the effect of water pumping and cleaning. The water storage tank stores water for heating, and air pressure is continuously injected into the water storage tank using an air pump.
Owner:ZHEJIANG UNIV

Low-irritation comfrey oil nanoliposomes, diaper rash preparation and application thereof in diaper rash of infants and young children

The application discloses a low-irritation borage oil nano-liposome, a diaper-care preparation and application of the diaper-care preparation in diaper rash of infants and young children, and belongs to the field of infant skin-care preparations. The nano-liposome is composed of the following components in percentage by weight: 0.5-2.0% of biodecolorized borage oil, 1.0-3.0% of chitosan oligosaccharide-ceramide binary membrane material, 0.3-1.0% of prebiotic skin flora regulator, 0.8-1.5% of pH 5.5 weak acid buffer system, 0.05-0.2% of edible-grade microencapsulated menthol, and the balance of sterile water for injection. The application also provides a diaper-care preparation containing the low-irritation borage oil nano-liposome, which is suitable for daily prevention and acute-phase intervention of infants and young children aged 0-36 months, has the characteristics of high bioavailability, low irritation, rapid repair and easy carrying, and has important clinical application value.
Owner:SHANGHAI PUTUO DISTRICT CENT HOSPITAL

Method for establishing a tissue culture sterile system of peach rootstock dormancy material

The present application relates to the technical field of plant tissue culture, and specifically discloses a method for establishing a tissue culture sterile system of peach rootstock dormancy material, which comprises the following steps: S1, selecting one-year-old branches with leaf buds in the dormancy period, removing flower buds and cleaning; S2, performing low-temperature treatment on the branches, then performing water culture and bud breaking to obtain sprouting branches; S3, performing surface sterilization treatment on the sprouting branches, and then washing with sterile water; S4, peeling the sprouting buds from the branches under sterile conditions, removing the external wrapping tissues of the bud bodies, and exposing the internal tender tissues; S5, performing secondary sterilization treatment on the exposed tender tissues, and then washing with sterile water; and S6, inoculating the bud bodies subjected to the secondary sterilization treatment into a culture medium for culture, and establishing a sterile system; according to the above method, in the process of establishing the sterile system of Zhongtao Kangzhen No. 1 material, the sterilization survival rate of 100 bud heads is as high as 80% or more, and the work efficiency and the utilization efficiency of the material are greatly improved.
Owner:LINYI AGRIFA SEEDS CO LTD

A method for preparing a microalgal extract

The present application belongs to the technical field of extract preparation, and particularly relates to a preparation method of a microalgae extract. The preparation method of the microalgae extract comprises the following steps: (1) adding sterile water to microalgae in a logarithmic growth phase to obtain a microalgae mixture; (2) spraying the microalgae mixture of step (1) on the surface of a culture carrier, and then adding the culture carrier to a microalgae culture medium added with ammonium niobate for culture, and collecting a product; (3) adding the product of step (2) to an ethanol aqueous solution, heating and refluxing for extraction to obtain an extract, and concentrating and drying the extract to obtain the microalgae extract. By using a culture carrier in the culture process of microalgae and adding an ammonium niobate component to the microalgae culture medium, the content of protein and polysaccharide in the prepared microalgae extract is obviously increased.
Owner:YACHUN (GUANGZHOU) BIOTECHNOLOGY CO LTD +3

Portable dialysate generator

A man-portable dialysate generator with a forward osmosis membrane module, a concentrated aqueous salt solution reservoir, a non-sterile water feedstock inlet, and a collection reservoir that produces sterile dialysate from non-sterile feed water. The dialysate generator does not have an electric-powered pump to move fluids across the osmosis membrane, or does not have electric-powered pumps that generate more than 5 psi for fluid pressure. The dialysate generator is man-portable, less than 50 pounds and maybe less than 31 pounds, and preferably less than 10 pounds. The device is particularly advantageous for use in forward deployed military patient care, disaster response or remote medical care situations away from clean water and electricity.
Owner:TDA RESEARCH INC

Gastrointestinal lens anti-fog structure

PendingCN122123629AGastroscopesOesophagoscopesBlurred visionMedicine
This invention discloses an anti-fogging structure for gastrointestinal endoscope lenses, belonging to the field of endoscopy technology. The anti-fogging structure includes an instrument body. A controller, adjustment mechanism, and water supply mechanism are fixedly mounted on the surface of the instrument body. A connecting tube is fixedly connected to the surface of the instrument body, and a probe is fixedly connected to the end of the connecting tube. The connecting tube and the probe are used to insert into human organs. The adjustment mechanism is used to adjust the position of the probe. The lens body is fixedly mounted on the surface of the probe. A heating tube is tightly fitted to the surface of the lens body, forming a highly efficient heat conduction structure. When sterile water is delivered to the heating tube through the outlet tube, the heating tube rapidly heats up and evenly transfers heat to the surface of the lens body, avoiding blurred vision caused by fogging. Furthermore, using sterile water as the medium avoids the risk of secondary contamination and meets medical-grade safety standards, balancing functionality and hygiene requirements.
Owner:GENERAL HOSPITAL OF PLA

Sterilization method in aseptic filling machine chamber

When the COP processing and the SOP processing in the chamber of the aseptic filling machine are performed, the cleaning solution or the sterile water remaining in the chamber before the sterilizing agent containing peracetic acid or hydrogen peroxide is sprayed is promptly removed. The conveying device that conveys the container in the chamber of the aseptic filling machine is driven to remove the cleaning solution after the COP processing or the sterile water during the SOP processing.
Owner:DAI NIPPON PRINTING CO LTD

Novel tetragonococcus strain for promoting growth of hermetia illucens larvae and application of novel tetragonococcus strain

The invention provides a new strain for promoting growth of larvae of hermetia illucens and application of the new strain. The strain comprises a 16S rDNA (ribosomal deoxyribonucleic acid) sequence shown in SEQ ID NO: 1 or a complementary sequence of the 16S rDNA sequence or a sequence with at least 85% of nucleic acid identity compared with the sequence shown in SEQ ID NO: 1. The Tesaracoccus sp. Tess.M30 disclosed by the invention has the characteristics of being capable of decomposing starch and resisting salt. The functional bacteria are added in the breeding process of the hermetia illucens, so that growth of larvae can be promoted, and accumulation of protein and fat of the larvae is promoted. Compared with sterile water, after the Tess.M30 is added, the body length and body weight of the hermetia illucens are respectively increased by 10.82% and 48.80%, and the accumulation of protein and fat is respectively increased by 61.65% and 76.80%. By applying the technology, the biotransformation efficiency of the hermetia illucens can be improved, and industrial production of larvae of the hermetia illucens can be promoted.
Owner:WESTLAKE UNIV +1