Production method of milk rich in galacto-oligosaccharides

A technology of galacto-oligosaccharides and production methods, which is applied in the direction of dairy products, milk preparations, food preparation, etc., can solve problems such as the decline of lactase activity, reduce production costs, solve lactose intolerance, and simplify cleaning and disinfection Effect

Inactive Publication Date: 2009-04-01
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with age, the activity of lactase in the human body decreases

Method used

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  • Production method of milk rich in galacto-oligosaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. The method of immobilizing β-galactosidase: first take a certain amount of particles with hydrazide groups, then add glutaraldehyde with a concentration of 2% and let it stand for 0.5-1 hour, the hydrazide groups of the carrier React with the aldehyde group of glutaraldehyde to generate an activated carrier, then wash the glutaraldehyde remaining on the activated carrier with sterile water, add β-galactosidase and let it stand for 2-4 hours, the aldehyde group of the activated carrier and β- Amino groups of galactosidase are combined to form immobilized β-galactosidase, and the remaining β-galactosidase is washed with sterile water to obtain immobilized β-galactosidase. Among them, the activity of the immobilized enzyme β-galactosidase is 60u / g.

[0038] 2, referring to Fig. 1, the present embodiment provides a kind of production method of above-mentioned galacto-oligosaccharide milk, comprises the steps:

[0039] (1) Clean the raw milk and cool it to 2-8°C for stor...

Embodiment 2

[0049] 1. The same method as in Example 1 was used to prepare immobilized β-galactosidase, wherein the activity of the immobilized enzyme β-galactosidase was 120 u / g.

[0050] 2, the present embodiment provides another kind of production method of above-mentioned galacto-oligosaccharide milk, comprises the steps:

[0051] (1) Clean the raw milk and cool it to 2-8°C for storage;

[0052] (2) heating to 50°C, separating the fat to obtain skim milk, the fat content of which is less than 0.5%;

[0053] (3) Skim milk is pasteurized, the sterilization temperature is 85°C, and it is kept warm for 15Sec; it is cooled to 4°C

[0054] (4) Then pump into the immobilized enzyme reaction column. The reaction column has a diameter of 150mm and a length of 450mm. hydrolysis. The hydrolysis temperature is 4°C, the hydrolysis time is controlled at 180 minutes, and the pH value of the hydrolysis environment is 7.0.

[0055] (5) UHT sterilization, the sterilization temperature is 137°C, the ...

Embodiment 3

[0061] 1. The same method as in Example 1 was used to prepare immobilized β-galactosidase, wherein the activity of the immobilized enzyme β-galactosidase was 150 u / g.

[0062] 2, the present embodiment provides another kind of production method of above-mentioned galacto-oligosaccharide milk, comprises the steps:

[0063] (1) Clean the raw milk and cool it to 2-8°C for storage;

[0064] (2) heating to 50°C, separating the fat to obtain skim milk, the fat content of which is less than 0.5%;

[0065] (3) Skim milk is pasteurized, the sterilization temperature is 85°C, and it is kept warm for 15Sec; cooled to 42°C

[0066] (4) Then pump into the immobilized enzyme reaction column. The reaction column has a diameter of 150mm and a length of 450mm. hydrolysis. The hydrolysis temperature is 42°C, the hydrolysis time is controlled at 90 minutes, and the pH value of the hydrolysis environment is 5.8.

[0067] (5) UHT sterilization, sterilization temperature 137 ℃, holding time 4S,...

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Abstract

The invention relates to a production method used for milk rich in galacto-oligosaccharide, comprising the steps as follows: heating milk, separating fat to get skim milk, pasteurizing, cooling, hydrolyzing by immobilizing Beta-galactosidase, UHT sterilizing, cooling and packaging. The carrier of the immobilized Beta-galactosidase is granular carrier with hydrazide group; immobilization method of the Beta-galactosidase comprises the steps as follows: the grains with the hydrazide group are added in glutaraldehyde and statically arranged for 0.5-1 hours; glutaraldehyde which is residual on the activation carrier is washed away by aseptic water; subsequently, Beta-galactosidase is added and statically arranged for 2-4 hours; the residual Beta-galactosidase is washed away by the aseptic water, thus gaining the immobilized Beta-galactosidase. The method greatly reduces the content of the lactose in the milk, meanwhile, the method generates galacto-oligosaccharide of high content; therefore, the method not only solves the problem of lactose intolerance but also adds galacto-oligosaccharide bifidus factor in the milk.

Description

technical field [0001] The invention relates to a method for producing milk rich in galacto-oligosaccharides, in particular to a method for producing milk rich in galacto-oligosaccharides by hydrolyzing lactose in milk with immobilized β-galactosidase, and belongs to the technical field of dairy products. Background technique [0002] Lactose is a unique sugar in milk. Under normal circumstances, lactose can be absorbed into the blood after being hydrolyzed into glucose and galactose under the action of lactase in human small intestinal epithelial cells. However, with age, the activity of lactase in the human body decreases. If too much lactose is ingested at one time, the lactose cannot be digested and absorbed in time, and after entering the colon, it will be decomposed by intestinal bacteria, producing a large amount of short-chain fatty acids such as lactic acid and formic acid and hydrogen gas, resulting in an increase in osmotic pressure and making the water in the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/15A23L1/29A23L33/00
Inventor 张少辉张勇宗学醒邢慧敏张萍付治军刘红霞桂仕林
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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