3176 results about "Oligosaccharide" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to applications of OpgG and a mutant thereof in production of human milk oligosaccharide. The invention provides a dextran biosynthetic protein OpgG mutant, and the OpgG mutant is obtained by mutating the 443rd amino acid from threonine to proline on the basis of a wild type dextran biosynthetic protein OpgG as shown in SEQ ID NO.1. The invention also provides a preparation method of the dextran biosynthetic protein OpgG mutant. An opgG gene and / or a T443P mutant coding gene are / is introduced into a human milk oligosaccharide production strain and applied to production of human milk oligosaccharide, and the yield of 3 '-SL, 6'-SL, LNT II, LNnT and LNT is greatly increased.

A synergistic composition for improving the bioaccessibility of minerals comprises lutein, a casein phosphopeptide, or a 1, 3-dioleoyl-2-palmitic acid triglyceride, preferably in combination with one or more non-digestible oligosaccharides. These compositions are useful for the prevention and / or treatment of mineral deficiency and related conditions, preferably anemia, said minerals comprising iron and / or calcium.

Provided herein are compositions that are useful in healing wounds and / or treating inflammatory skin conditions comprising (a) a copper-containing peptide; (b) an antioxidant; and (c) a cyclic oligosaccharide-based polymer, where the copper-containing peptide and the antioxidant form a complex. Further provided herein are methods for healing wounds and / or treating inflammatory skin conditions using the compositions of the disclosure.

The invention discloses N-acetylglucosamine transferase AcetRL-R349H and an application thereof, and belongs to the technical field of functional enzymes. The amino acid sequence of the N-acetylglucosamine transferase AcetRL-R349H is as shown in SEQ ID NO. 5 (sequence identifier number 5). The invention further discloses the application of the N-acetylglucosamine transferase AcetRL-R349H in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRL-R349H disclosed by the invention is obtained by carrying out mutation modification on the N-acetylglucosamine transferase AcetRL, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 4 to 6, especially (GlcNAc) 6. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The present invention relates to a nutritional composition, food and pharmaceutical products comprising the nutritional composition, and uses thereof. The nutritional composition comprises medium and long chain fatty acid triglycerides and breast milk oligosaccharides, and the weight ratio of the medium and long chain fatty acid triglycerides to the breast milk oligosaccharides is 1: 1 to 9: 1. The nutritional composition disclosed by the invention can play a role in synergistically promoting skeletal development and particularly improving bone mineral density.

The invention discloses an N-acetylglucosamine transferase AcetRS-R346D and an application thereof, and belongs to the technical field of functional enzymes. And the amino acid sequence of the N-acetylglucosamine transferase AcetRS-R346D is as shown in SEQ ID NO. 3. The invention further discloses the application of the N-acetylglucosamine transferase AcetRS-R346D in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRS-R346D disclosed by the invention is obtained by carrying out mutation modification on the N-acetylglucosamine transferase AcetRS, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 4 to 7, especially (GlcNAc) 7. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The invention discloses application of fucoidin degrading enzyme OUC-MgFucD1 in preparation of fucoidin oligosaccharide, and belongs to the technical field of preparation of the fucoidin oligosaccharide. The amino acid sequence of the fucoidin degrading enzyme OUC-MgFucD1 is as shown in SEQ ID NO. 1, and the fucoidin degrading enzyme OUC-MgFucD1 is as shown The fucose oligosaccharide is fucose, fucodisaccharide and fucotriose, the molecular formula of the fucose is C6H12O8S, the molecular formula of the fucodisaccharide is C12H22O15S2, and the molecular formula of the fucotriose is C18H32O16S. The invention further discloses a preparation method of the fucose oligosaccharide. The fucoidin degrading enzyme OUC-MgFucD1 disclosed by the invention can specifically act on fucoidin, and a specific fucoidin oligosaccharide combination is generated by precisely cutting a main chain glucosidic bond of the fucoidin. The invention provides a key technical support for the development of high-added-value fucoidan oligosaccharide products, and has important significance for promoting the industrial application of the fucoidan oligosaccharide in the fields of functional foods, biological medicines and the like.

The invention discloses a biochar-based slow-release fertilizer and a preparation method thereof, and relates to the technical field of fertilizer production, the biochar-based slow-release fertilizer comprises a core, a carrier layer, a biological layer and a protective layer, the core is composed of macroelements, microelements and a nitrification inhibitor; the carrier layer comprises graphene quantum dot modified charcoal, chitosan, glycerol and cyclodextrin grafted starch; the biological layer is prepared from magnetic Fe3O4 and humic acid nanoparticles, alginic acid oligosaccharide and diatomite; the protective layer comprises a polylactic acid-polycaprolactone block copolymer, acetone, calcium lignosulphonate, a dynamic covalent cross-linking agent gelatin, nano cellulose and an anti-blocking agent. Therefore, by adopting the polylactic acid-polycaprolactone (PLA-PCL)-based segmented copolymer and the dynamic covalent network cross-linking agent, the self-repairing property is endowed. The slow-release fertilizer provided by the invention realizes precise regulation and control of the fertilizer efficiency release rate through a multi-level synergistic effect, has the functions of soil remediation and stress resistance, and significantly improves the utilization efficiency of the fertilizer and the crop adaptability.

The invention belongs to the field of food, and particularly relates to a nutritional composition containing probiotics and breast milk oligosaccharide for improving anxiety or depression and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improving constipation and anxiety state and / or depression state caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a bifidobacterium probiotic, and (ii) a sialylated breast milk oligosaccharide; the bifidobacterium probiotics at least comprise animal bifidobacterium subsp. Lactis, the sialylated breast milk oligosaccharide at least comprises 6 '-sialylated lactose, and the animal bifidobacterium subsp. Lactis comprises a Bb-12 strain. The substance combination of the bifidobacterium probiotics and the sialylated breast milk oligosaccharide can synergistically improve abnormal brain-intestine interaction, and improve constipation and anxiety and / or depression state caused by constipation.

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention provides a fucosyl transferase polypeptide, which can be applied to the synthesis of human milk oligosaccharides (HMOs), and belongs to the technical field of enzyme engineering. The fucosyltransferase polypeptide provided by the invention comprises polypeptides with at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% same amino acid sequences as polypeptides shown in amino acid sequences such as SEQ ID NO: 1-25. According to the transferase polypeptide disclosed by the invention, the specificity of a polypeptide catalytic substrate is also improved while the ability of the polypeptide as shown in SEQ ID NO: 1 to catalyze and synthesize 2 '-FL is improved.

The invention discloses an ulva lactuca composite wall-breaking enzymolysis fermentation product as well as a preparation method and application thereof, and relates to the field of cosmetics. The preparation method comprises the following steps: soaking ulva powder, and performing physical cell wall breaking treatment; mixed enzymes are added for an enzymolysis reaction, wherein the mixed enzymes comprise lipase, cell wall breaking enzyme, enzyme and protease; adding the enzymolysis product into a culture medium, and inoculating bacterial liquid for fermentation culture to obtain fermentation liquid. According to the application, active ingredients such as macromolecular polysaccharide and protein in the ulva lactuca are extracted and decomposed by adopting physical cell wall breaking, composite enzymolysis and bifidus yeast fermentation technologies, and the prepared fermentation product is rich in various composite micromolecular active ingredients such as oligosaccharide, polypeptide, amino acid, polyphenols and flavonoids, has high free radical scavenging capacity, and can be used for preparing the ulva lactuca beverage. The anti-wrinkle and anti-oxidation composition has good anti-wrinkle and anti-oxidation effects, is beneficial to skin absorption when being applied to cosmetics and has high absorption efficiency, and the prepared cosmetics have excellent anti-wrinkle, anti-oxidation, soothing and repairing effects.

The invention provides a method for producing pullulan by aureobasidium pullulans, which is characterized in that a microbial conversion method is adopted for the first time, and pullulan is produced by catalyzing conversion of fructooligosaccharide through an enzyme reaction liquid, and the pullulan is produced by taking an aureobasidium pullulans seed culture solution as a carrier, fructooligosaccharide as a substrate and the enzyme reaction liquid as a catalyst. In a 5.0 L biological reaction container, a mode of one-time substrate addition or multiple feeding of fructooligosaccharide and enzyme reaction liquid is adopted, catalytic reaction is performed for 60-72 hr, 61.43 g / L-71.43 g / L of fructooligosaccharide is consumed together, the highest yield of pullulan is 60.2 g / L-64.26 g / L, and the conversion rate of fructooligosaccharide is up to 87.5%-97.98%. Compared with the existing method for producing the pullulan by a fermentation method, the biotransformation method can solve the problem of insufficient enzyme amount of catalytic reaction depended in the pullulan synthesis process on one hand, and also can solve the problem of reverse regulation of the substrate and the product on pullulan synthesis in the pullulan synthesis process on the other hand; and the problems of low substrate carbon source utilization rate, low substrate conversion rate and the like caused thereby are solved.

The invention provides a soothing and repairing composition for oil-sensitive skin and application of the soothing and repairing composition, and belongs to the technical field of cosmetics. According to the soothing and repairing composition, the hydrolyzed yeast protein, the aesculus chinensis extract, the marine oligosaccharide, the Australian tealeaf extract and the phellinus igniarius extract are compounded to form the soothing and repairing composition, and the soothing and repairing composition reduces skin discomfort by maintaining the steady state of an enzyme network of epidermis, reducing oxidase and increasing enzymes and defensive enzymes for promoting Newcastle metabolism; the effects of repairing, moisturizing, controlling oil and relieving are achieved, the skin injury of oily sensitive skin is gently repaired, and the health state of the skin is recovered.

According to the high-gel-quality minced fillet gel and the preparation method thereof, frozen minced silver carp is unfrozen and then subjected to ice-temperature vacuum dehydration and ultrahigh-pressure homogenization treatment, a myofibrillar protein molecular chain is promoted to stretch through mild shearing force, and a TG enzyme acting site is fully exposed; then chopping and mixing with table salt, starch, TG enzyme coated porous starch-curdlan / sodium alginate oligosaccharide nanosphere dispersion liquid and ice water at low temperature, shielding TG enzyme by nanospheres through a core-shell structure at low temperature so as to avoid advanced activation, and accurately releasing during heating so as to catalyze crosslinking; finally, step-by-step heating gelation is performed, so that the strength and uniformity of minced fillet gel are remarkably improved, the flavor is improved, and the method is suitable for industrial production.

The invention discloses oligochitosan oligosaccharide, oligochitosan oligosaccharide composite nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of nano materials and food engineering. According to the method disclosed by the invention, the chitosan oligosaccharide with the polymerization degree of 2-6 is prepared by adopting an ultrasonic-microwave synergistic compound enzyme enzymolysis method, so that the yield and the quality of the chitosan oligosaccharide are improved. The chitosan oligosaccharide and zein are assembled to form the chitosan oligosaccharide composite nanoparticles, the chitosan oligosaccharide composite nanoparticles have the advantages of being small in particle size and high in stability, the antioxidant function of the chitosan oligosaccharide is remarkably improved, the preparation method is simple, convenient, economical and practical, and the chitosan oligosaccharide composite nanoparticles can be used as food, health care products and medicine carrier materials and have good market application prospects.

A nutritional composition, typically an infant enterically administered nutritional composition, that comprises a milk fat globule membrane component, at least one human milk oligosaccharide and one additional component or a plurality of additional components where the additional component or components include DHA, ARA, Vitamin E, Vitamin C, and sphingomyelin.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention relates to the field of nutrient solutions, and particularly discloses a high-calcium-zinc dietary nutrient solution for promoting skeletal development and a preparation method thereof, the high-calcium-zinc dietary nutrient solution for promoting skeletal development comprises the following raw materials: calcium citrate, zinc citrate, a zinc-containing yeast extract, vitamin D3, a nutrition enhancer, functional oligosaccharide, xanthan gum, anhydrous citric acid, essence, a sweetening agent and water; the preparation method comprises the following steps: mixing the zinc citrate, the zinc-containing yeast extract, the seaweed meal, the natto powder, the stachyose and the trehalose to prepare a mixture A; uniformly premixing stevioside, xanthan gum and trehalose to obtain a mixture B; s4, vitamin D3 powder and trehalose are premixed and dissolved, and a mixture C is prepared; heating the residual water, adding the mixture A, the fructo-oligosaccharide, the anhydrous citric acid, the calcium citrate, the mixture B, the trehalose and the mixture C, adding the essence, and stirring to obtain the beverage. The preparation method has the characteristic of preparing the liquid calcium-zinc dietary nutrient solution with good absorption and utilization rate and palatability.

The invention relates to the technical field of flower planting substrates, and discloses a general type flower fertilization-free planting substrate based on nitrogen-fixing, phosphorus-dissolving and potassium-dissolving bacteria, which comprises turfy soil, perlite, ground phosphate rock, straw, carbonized rice husk, humic acid, seaweed oligosaccharide, nitrogen-fixing bacteria, phosphorus-dissolving bacteria and potassium-dissolving bacteria. Turfy soil and perlite are used as a substrate and compounded with ground phosphate rock, straw, carbonized rice husks, humic acid, seaweed oligosaccharide, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria, the nitrogen-fixing bacteria provide nitrogen for flowers, and the phosphate solubilizing bacteria and the potassium solubilizing bacteria decompose phosphorus and potassium in the ground phosphate rock, the straw and the carbonized rice husks to provide nutrition for the flowers; the humic acid and the seaweed oligosaccharide have the effects of promoting rooting and growth of the flowers and breeding of beneficial microorganisms; all the internal components have a synergistic effect, the fertilization-free period is extremely long, essential elements for plants are still efficiently provided under the condition of no fertilization, the growth of the plants and the development of flowers are remarkably promoted, the use of toxic substances is reduced, meanwhile, the low incidence rate of diseases and insect pests is ensured, and better flower growth and ornamental effects are achieved on the whole.

The invention discloses an oligosaccharide enzyme fermentation process control system and method based on pH and dissolved oxygen electrode feedback, relates to the technical field of oligosaccharide enzyme fermentation, and aims to improve the yield and activity of oligosaccharide enzyme. The system comprises a pH electrode, a dissolved oxygen electrode, a stirrer, a ventilation system and a temperature control system, and accurate control is realized through a data acquisition and processing unit, a logic control unit and a human-computer interface. The control method comprises the steps of seed culture, shake flask fermentation, fermentation tank culture, monitoring and evaluation as well as optimization and adjustment. Through a segmented oxygen control strategy and dissolved oxygen and stirring linkage control, the dissolved oxygen level is accurately controlled, the yield and activity of the oligosaccharide enzyme are remarkably improved, the fermentation process conditions are optimized, and the monitoring and evaluation capability is enhanced. The method is suitable for industrial production of the oligosaccharide enzyme, and is beneficial for improving the product quality and the economic benefit.

The invention relates to the technical field of compound fertilizers, and discloses a boron-zinc-molybdenum synergistic compound fertilizer, which is prepared from the following raw materials in parts by weight: calcium nitrate, ammonium polyphosphate, potassium sulfate, magnesium sulfate, disodium octoborate tetrahydrate, sugar alcohol chelated zinc, sodium molybdate and a synergistic auxiliary agent, the feed additive is prepared from a feed additive, an activator, 5-alginic acid oligosaccharide, aminolevulinic acid, sugar alcohol chelated magnesium, an antagonist, a stress-resistant enhancer and a coating material. According to the boron-zinc-molybdenum synergistic interaction compound fertilizer and the preparation process thereof, the disodium octoborate tetrahydrate, the sugar alcohol chelated zinc and the sodium molybdate are compounded according to a specific proportion, antagonism is avoided through proportion limitation, the synergistic effect of the three elements is remarkably enhanced, and the problem that a single element is insufficient or excessive is solved; chelated elements such as sugar alcohol chelated zinc and sugar alcohol chelated magnesium are combined with an activating agent (humic acid chelated calcium and silica sol) to form a gel-state chelate, so that the trace elements can be prevented from being fixed by soil, and the transfer efficiency of the trace elements in crops is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Longum as well as a composition, a pharmaceutical composition, a culture, a food product or a dietary supplement containing the bifidobacterium longum subsp. Longum and application of the bifidobacterium longum subsp. Longum. The bifidobacterium longum is preserved in the Guangdong Microbial Culture Collection Center on January 21, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.65836. The bifidobacterium longum subsp. Longum has relatively strong gastric acid and cholate tolerance and intestinal colonization capability; the bifidobacterium longum subsp. Longum can effectively utilize bifidobacterium bifidum to release monosaccharide or disaccharide outside cells for efficient proliferation, and the composition containing the bifidobacterium longum subsp. Longum, the bifidobacterium bifidum and breast milk oligosaccharide can release a large amount of short-chain fatty acid, so that the effects of maintaining the physiological function of an organism and improving the health of the organism are achieved.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention discloses a camellia nitidissima flower extract-containing anti-pollution acne-sensitive skin repairing composition and application thereof, and relates to the technical field of cosmetics. The invention provides a camellia nitidissima flower extract-containing anti-pollution acne-sensitive skin repairing composition. The camellia nitidissima flower extract-containing anti-pollution acne-sensitive skin repairing composition is prepared from the following components in parts by weight: 1 to 5 parts of camellia nitidissima flower extract, 1 to 5 parts of seaweed oligosaccharide, 0.01 to 1 part of o-cymene-5-ol and 0.01 to 1 part of tocopherol retinoate. According to the anti-pollution acne-sensitive skin repairing composition containing the camellia nitidissima flower extract, four components including the camellia nitidissima flower extract, the seaweed oligosaccharide, the o-cymene-5-ol and the tocopherol retinoate in proper parts by weight are selected to cooperate with one another, so that the composition has better anti-pollution and antibacterial effects and has very good acne removing, repairing and soothing effects.

The invention belongs to the technical field of gene engineering, and particularly relates to an RNA polymerase beta subunit mutant and application thereof. The RpoB mutant is obtained through a gene editing technology, after the 560th amino acid of the RpoB is changed into leucine from proline, the number of living cells during strain fermentation is greatly increased, the tolerance of the RpoB mutant in the later environment of a fermentation tank is improved, the RpoB mutant is applied to escherichia coli for producing human milk oligosaccharide, and finally the yield of the human milk oligosaccharide is greatly increased.

The invention discloses ice glazing liquid for ice glazing of mussels. The ice glazing liquid is prepared from the following components in percentage by mass: 0.1 to 0.12 percent of sodium polyacrylate, 0.05 to 0.07 percent of sodium erythorbate, 0.01 to 0.03 percent of citric acid, 1 to 1.2 percent of sodium lactate, 0.4 to 0.6 percent of alginate oligosaccharide, 0.1 to 0.12 percent of xanthan gum, 7 to 9 percent of propylene glycol and the balance of water. The ice glaze liquid for ice glaze plating of the mussels is reasonable in compatibility, simple and easily available in components and good in adhesiveness, the formed ice glaze is not easy to crack, the dry loss rate can be reduced, and the quality of the thawed mussels can be ensured. The invention further provides a preparation method of the ice glaze liquid for ice glaze plating of the mussels. The preparation method is simple in process step, high in operability and uniform in component dispersion.

The invention relates to the technical field of fertilizers, in particular to an environment-friendly water-soluble fertilizer containing a seaweed extract and polyglutamic acid. The water-soluble fertilizer is prepared by preparing seaweed oligosaccharide and double-grafted poly-L-gamma-sodium glutamate and compounding and complexing the seaweed oligosaccharide and the double-grafted poly-L-gamma-sodium glutamate, has the characteristics of low salting-out, high stability, no precipitation, good slow release performance and the like, and can continuously release nutrients and keep a solution clear and transparent under a low-salt condition; and because the polyglutamic acid and seaweed extract compound drip irrigation agent has the chelation and water retention characteristics of the polyglutamic acid and the natural growth promoting effect of the seaweed extract, the crop rhizosphere nutrient absorption efficiency and stress resistance are remarkably improved, the compound drip irrigation agent is suitable for drip irrigation and water and fertilizer integrated application of greenhouse vegetables, fruit trees and field crops, and the comprehensive effects of high efficiency, low salt load and environment friendliness are achieved.

The invention provides a fertilizer synergist for reducing nutrient loss and inhibiting nitrification and application of the fertilizer synergist. The fertilizer synergist is prepared from the following components in percentage by mass: 95 to 98 percent of carboxyl cross-linked modified montmorillonite, 0.05 to 0.1 percent of mannan oligosaccharide, 0.1 to 0.5 percent of mannan, 1 to 3 percent of polyaspartate and 0.5 to 2 percent of a nitrification inhibiting aid. According to the fertilizer synergist capable of reducing nutrient loss and inhibiting nitrification and the application of the fertilizer synergist, the fertilizer synergist serves as a plant nutrient loss control synergist and a nitrification inhibitor and can activate a crop stress resistance signal source, promote and induce synthesis of endogenous auxin in plants, root and growth, activate phosphorus and dissolve potassium, reduce nitrogen loss and improve the yield of the plants. The fertilizer utilization rate is improved.

The invention discloses a kind of eucommia leaf fermentation extract and its preparation method and application, and belongs to the technical field of microorganisms and feed additives. With eucommia leaf and wheat bran as substrates, by configuring a specific black aspergillus niger-trichoderma harzianum-saccharomyces cerevisiae complex bacterial liquid as a fermentation agent, a closed fermentation is carried out to make black aspergillus, trichoderma harzianum, and saccharomyces cerevisiae ferment to produce a large amount of cellulase, protease, lipase, amylase and glucosidase, etc., and then by pumping and circulating treatment, the cellulose, protein, starch, beta-glycan and other macromolecular substances in eucommia leaf are further enzymolyzed into protein oligopeptides, monosaccharides, and soluble oligosaccharides that are beneficial to livestock and poultry absorption. The eucommia leaf fermentation extract prepared by the present invention is used as a feed additive for beef cattle feeding, improves the daily weight gain and feed intake of beef cattle, and significantly reduces the feed-to-meat ratio, saves feed consumption, improves the protein content in meat, reduces the fat content, reduces energy, and improves meat quality.