609 results about "Folic acid" patented technology

The invention relates to the technical field of liquid chromatography-mass spectrometry series connection, and particularly discloses a method for detecting folic acid metabolites of red blood cells through the liquid chromatography-mass spectrometry series connection technology. And an image analysis algorithm based on artificial intelligence and deep learning is utilized to extract a chromatographic separation state, so that gradient parameters are dynamically adjusted, the requirement of manual intervention is reduced, and the separation process of a target object in a complex matrix is favorably optimized. Particularly, key features in a chromatogram can be automatically identified and quantified based on a deep learning method, and gradient elution parameters are intelligently and dynamically adjusted according to the information, so that more optimized substance separation and detection can be realized. For example, if separation of a certain component is not ideal, the separation effect of the component can be improved by adjusting the proportion of the mobile phase B, so that the gradient elution process is optimized.

The invention discloses a fermentation medium for regulating and controlling a clostridium aerovorans target product and a fermentation method of the clostridium aerovorans target product. The medium comprises a basic component and a regulating component, the basic components comprise ammonium chloride, potassium chloride, magnesium sulfate heptahydrate, sodium chloride, monopotassium phosphate, yeast powder, calcium chloride dihydrate, sodium selenite, nickel chloride hexahydrate, biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, D-calcium pantothenate, cobalamin, para aminobenzoic acid and lipoic acid; the adjusting component comprises sodium tungstate, ferrous sulfate and sodium molybdate, sodium tungstate and ferrous sulfate have a forward adjusting effect on increasing the yield of acetic acid, ethanol and butanol in the target product, and sodium molybdate has a reverse adjusting effect on the target product. By optimizing the proportion of trace elements in the culture medium, the clostridium metabolism efficiency is improved, synthesis of a target product is regulated and controlled, and the method is particularly suitable for industrial production of acetic acid, ethanol, butanol and the like.

The invention discloses collagen polypeptide firming and anti-wrinkle freeze-dried powder, and relates to the technical field of cosmetics. The invention relates to a biological preservative which is prepared from water, butanediol, a bacillus / soybean fermentation product extract, soluble collagen, acetyl hexapeptide-8, panthenol, 1, 2, 4-trimethyl-1, 3-pentanediol and a proper amount of water. The cosmetic is prepared from 1, 2-hexanediol, tetrahydromethylpyrimidine carboxylic acid, hydroxypropyl tetrahydropyrantriol, tremella polysaccharide, saccharide isomeride, a bifidus yeast fermentation product lysate, sodium hyaluronate, DNA sodium, a radix gentianae extract, mannitol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, oligopeptide-1, decapeptide-4, oligopeptide-3, hydrolyzed royal jelly protein, folic acid and disodium hydrogen phosphate. The additive is composed of sodium dihydrogen phosphate, citric acid and sodium citrate. The mask can provide rich nutrients for skin, deeply care the skin, moisturize and relieve the skin, tighten the skin, resist wrinkles and make the skin moisturized, glossy, elastic and tight, and is high in use safety and wide in application prospect.

The compositions comprise at least two ingredients selected from the group consisting of fish oil, L-arginine, vitamin E, vitamin C, vitamin B3, vitamin B5, vitamin B6, vitamin B1, vitamin B2, folic acid, biotin, vitamin B12, and are formulated for the prevention and / or treatment of a disease or condition associated with muscle decline in a subject in need thereof. Preferably, the subject in need is an individual at risk of or having renal failure, renal dysfunction or / and nephropathy, such as chronic nephropathy, protein energy consumption, end-stage nephropathy, renal failure due to hospitalization in an intensive care unit, acute kidney injury, and the muscle decline is selected from the group consisting of muscle depletion, muscle loss due to renal failure or dysfunction, muscle decline associated with chronic kidney disease, muscle loss due to hospitalization in intensive care units during renal failure, metabolic acidosis during chronic kidney disease, protein energy depletion, or a combination thereof. The method enables the prevention and / or treatment of at least one outcome of a disease or condition associated with muscle decline in a subject at risk of or having renal failure, renal dysfunction or / and kidney disease. The composition and the method can be used to improve the level of at least one amino acid.

The embodiment of the invention provides breast cancer targeting nanoparticles as well as a preparation method and application thereof. According to the embodiment of the invention, the breast cancer targeting nanoparticles are synthesized by preparing the pH sensitive polymer, carrying out co-coupling modification on the pH response polymer and the folic acid albumin nano-carrier and utilizing a controlled desolvation method; the breast cancer targeting nanoparticle is combined with a nano preparation passive targeting strategy, a tumor microenvironment stimulation response strategy and a folic acid active targeting strategy, the effects of an EPR effect, PEG shedding, charge overturning, folic acid ligand-receptor combination and the like are utilized, precise targeting and specific response of tumors are achieved, and targeted accumulation of drugs in a tumor microenvironment is comprehensively improved. Therefore, the breast cancer targeting nanoparticles prepared by the embodiment have the advantages of high targeting property, drug controlled release, improvement of solubility and absorptivity of indissolvable drugs and the like, so that the curative effect of the drugs can be enhanced, the toxic and side effects can be reduced, and the breast cancer targeting nanoparticles have wide application prospects in the field of anti-tumor treatment.

This application relates to the field of food microbiology, and specifically discloses a Lactobacillus plantarum capable of producing 5-methyltetrahydrofolic acid, its fermentation method and application. A Lactobacillus plantarum inm30-LP capable of producing 5-methyltetrahydrofolic acid, with a preservation date of December 26, 2022, and the preservation unit is the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 26278. This application is based on the milk curd in the Gannan Tibetan area as the food source, isolates probiotics, and screens out Lactobacillus plantarum with high folic acid production from them. Moreover, the strain has obvious advantages over other lactic acid bacteria in terms of acid resistance and bile salt resistance, is suitable for growing in the gastrointestinal environment, has the ability to proliferate, has no antibiotic resistance, has antibacterial activity, and can produce bacteriocin.

The invention relates to the fields of biology, medicine and clinic. In particular to application of PAICS as a target spot in developing or screening reagents for preventing and / or treating orthopoxvirus infection, and application of inhibiting a synthetic pathway of purine nucleotide and / or inhibiting a metabolic pathway of folic acid in preparing reagents for treating and / or preventing orthopoxvirus infection diseases. In order to restrain the infection of orthopoxvirus, the inventor team uses a CRISPR / Cas9 technology to carry out generic genome screening on host cells infected by orthopoxvirus, determines a host-dependent gene PAICS gene in the orthopoxvirus infection process, and verifies and clarifies the virus-dependent mechanism of the gene; in order to deal with the global continuous orthopoxvirus infection problem, the invention plays an important role in the development and screening of future related prevention or treatment drugs, and also expands the new clinical application of existing drugs including methotrexate, mercaptopurine, mizoribine and the like.

The invention provides geranial dehydrogenase as well as a preparation method and application thereof, and relates to the technical field of biology, and the amino acid sequence of the geranial dehydrogenase is as shown in SEQ ID NO.1. Citral can be used as a substrate to catalytically synthesize the aromatic folic acid, and the conversion rate reaches 90%. The geranial dehydrogenase can be used for efficiently synthesizing the aromatic folic acid and can be used for biocatalytically converting the aromatic folic acid. Meanwhile, NAD < + > is used as a coenzyme to improve the enzymatic reaction efficiency, so that efficient catalytic synthesis of the aromatic folic acid is realized.

The invention belongs to the field of food, and particularly relates to application of a nutritional composition for assisting in improving memory ability. The invention provides application of a nutritional composition in preparation of food for assisting in improving memory ability of offspring. The nutritional composition comprises the following necessary components: active folic acid substances and fucosyllactose, moreover, in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015): (0.5-10), the food is ingested by a mother to play the role of assisting in improving the memory ability of the offspring, the mother is in a gestation period and / or a lactation period, and the maternal is in the gestation period and / or the lactation period. The filial generation comprises a fetal-stage filial generation and / or an infant-stage filial generation and an optional child-stage filial generation. A large number of studies find that when active folic acid substances and fucosyllactose which are combined according to a certain proportion are supplemented to a mother at the beginning of the gestation period, the memory ability of offspring can be remarkably improved in an auxiliary mode.

The invention relates to the technical field of biological medicine, and discloses a culture medium for human metapneumovirus and application, the culture medium comprises the following components by mass: 850-920 parts of a basic culture medium; the metabolism reprogramming module is prepared from the following components in parts by weight: 0.05 to 0.15 part of 5-methyltetrahydrofolic acid, 0.3 to 0.7 part of N-acetyl-D-mannosamine and 1.0 to 3.0 parts of sodium dichloroacetate; the immune regulation and control module is prepared from 0.005 to 0.015 part of an RIG-I / MAVS pathway inhibitor; the receptor induction module is prepared from 0.5 to 1.5 parts of all-trans retinoic acid; the redox balance module is prepared from the following components in parts by weight: 0.1 to 0.5 part of pegylated superoxide dismutase and 0.002 to 0.008 part of sodium selenite. According to the method, the proportion of infectious virus particles can be remarkably increased, the generation of defective particles is reduced, meanwhile, the production cycle of host cells is prolonged, large-scale continuous culture is supported, and a high-quality and high-efficiency solution is provided for industrial production of gene therapy vectors and virus vaccines.

The present disclosure relates to a field of biomedical technology. Embodiments of the present disclosure provide a leonurine nanocomposite hydrogel and preparation method and application thereof, including: dissolving dopamine (DA) monomers and inducing oxidation and self-polymerization of the DA to obtain polydopamine (PDA) nanoparticles, grafting thiol polyethylene glycol folic acid (SH-PEG-FA) on a surface of the PDA nanoparticles to obtain folic acid polydopamine (FA-PDA) nanocarriers; loading a leonurine (Leon) using the FA-PDA nanocarriers of the step 1 to obtain FA-PDA@Leon; encapsulating the FA-PDA@Leon of the step 2 into a gel matrix to obtain gel@FA-PDA@Leon hydrogel.

The invention belongs to the technical field of biological detection, and particularly relates to a method for synchronously and quantitatively detecting various folic acid metabolism related substances in trace plasma or serum, which comprises the following steps of: detecting a pretreated trace plasma or serum sample by adopting a liquid chromatography-tandem mass spectrometry method; the following nine folic acid metabolism related substances are synchronously and quantitatively detected: 5-formyl tetrahydrofolic acid, folic acid, 5-methyl tetrahydrofolic acid, S-adenosine-L-methionine, S-adenosine-L-homocysteine, vitamin B12, pyridoxamine, pyridoxine and pyridoxal. The invention further relates to application of the detection method in related detection of child neurodevelopment. According to the detection method, only trace plasma or serum is used, methotrexate is used for replacing an expensive isotope internal standard for more economical detection, and nine folate metabolism related substances can be accurately quantified at the same time. Therefore, the detection method disclosed by the invention has important clinical significance and wide market application prospect.

The invention relates to the technical field of cell culture. The invention provides a mesenchymal stem cell culture medium without animal-derived components and application of the mesenchymal stem cell culture medium. The culture medium comprises a basic culture medium and an additive, the additives comprise FGF-2, IGF-1, glutamine, folic acid, serotonin, cholesterol, arachidonic acid, an HEPES buffer solution, sodium bicarbonate, SCF, TGF-beta, PDGF, ascorbic acid and transferrin. The mesenchymal stem cell culture medium disclosed by the invention is completely free of animal-derived components, and meets the requirements of FDA / EMA clinical-grade cell therapy products. The culture medium can support high-density amplification of mesenchymal stem cells, the activity of passage cells is effectively improved, and the amplification time is shortened. The method has remarkable significance in improving the amplification efficiency of the mesenchymal stem cells and promoting and applying factory growth.

The present invention discloses a yellow-green fluorescent COFs material, its preparation method and application, belonging to the fields of covalent organic framework materials and analytical detection. In the present invention, 4,4',4'',4'''-(ethene-1,1,2,2-tetrayl)tetraphenylaldehyde (ETBA) and a flexible organic monomer 4,4'-diaminodiphenyl ether (ODA) with chelating sites are used as raw materials, methanol and acetic acid are added and shaken for dispersion, frozen with liquid nitrogen, evacuated and reacted in a constant temperature oven, the product is collected by filtration, washed with anhydrous N,N-dimethylformamide and tetrahydrofuran respectively, and then centrifuged and washed with anhydrous acetone, the solid product is collected, dried in an oven to obtain yellow ETBA-ODA-COF powder. The present invention regulates the π-π interaction in the COF structure, synthesizes a kind of ETBA-ODA-COF material with yellow-green fluorescence, and can establish a fluorescent nanoprobe to realize the analysis and detection of metal Fe 3+ , Hg 2+ ions and folic acid small molecules.

The invention discloses a feed for improving the flavor of broiler chickens and a preparation method of the feed, and belongs to the technical field of poultry feeds and feed preparation. Comprising the following components in parts by weight: 55 to 65 parts of basic ration, 35 to 40 parts of sea buckthorn pomace and bean dreg combined ferment, 3 to 5 parts of aquatic product leftover enzymatic hydrolysate, 3.5 to 4.0 parts of trehalose, 1.0 to 1.5 parts of ground cinnamon, 1.0 to 1.5 parts of xylooligosaccharide, 0.8 to 1.0 part of choline, 0.0001 to 0.0002 part of organic selenium and 0.01 to 0.02 part of folic acid. The mass ratio of the choline to the aquatic product leftover enzymatic hydrolysate is 1: (3.0-5.6); the mass ratio of the organic selenium to the sea buckthorn pomace and bean dreg combined fermentation product is 1: (58-100); the sea buckthorn pomace and bean dreg combined fermentation product is prepared by respectively pretreating sea buckthorn pomace and bean dregs and then carrying out aerobic, facultative and anaerobic continuous three-stage fermentation. The technical limitations that flavor substances of traditional feed are easy to inactivate and the utilization rate of agricultural by-products is low are broken through, and the breakthrough of flavor quality upgrading, efficient resource conversion and breeding benefit optimization is achieved.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention relates to an enzyme response antibacterial carbon dot and a preparation method and application thereof.The carbon dot takes a copper-doped carbon dot as a core and is covalently grafted with a hyaluronic acid shell layer through amidation reaction to form a composite nano material Cu-CDs (at) HA, specifically, soluble copper salt and folic acid are subjected to a hydrothermal reaction to obtain the copper-doped carbon dot, carboxyl is activated through EDC / NHS in a buffer system, and the carbon dot is prepared. And performing grafting reaction with hyaluronic acid to obtain a target product. The hyaluronidase-containing antibacterial composition can be specifically degraded and release antibacterial components under the action of hyaluronidase, shows good bacterial responsive bactericidal performance, has the minimum inhibitory concentrations of 8 g / mL and 16 g / mL for staphylococcus aureus and escherichia coli respectively, and has peroxidase-like activity and free radical scavenging capacity. The antibacterial carbon dots can be used for preparing intelligent wound dressings, antibacterial coatings and other biomedical materials, and have the advantages of responsive drug release, efficient antibacterial property and promotion of wound repair.

The use of a nutritional composition in the preparation of a food product for assisting in improving the memory ability in offspring, wherein the nutritional composition contains the following essential components: an active folic acid substance and fucosyllactose; and in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015):(0.5-10). The food product exerts the effect of assisting in improving the memory ability in offspring, wherein the food product is ingested by a mother, the mother is preganant and / or lactating, and the offspring comprises fetal offspring and / or infant offspring and optionally childhood offspring.

The invention discloses a dendrobium officinale cold-resistant agent and application thereof. The active ingredient of the cold-resistant agent is folic acid and / or fulvic acid. Folic acid and fulvic acid are applied to cold-resistant treatment of dendrobium officinale for the first time, the innovation breaks through the limitation that a traditional cold-resistant agent is complex in component and possibly contains hormone or pesticide components, and a new, safer and more effective choice is provided for cold-resistant treatment of dendrobium officinale. The cold-resistant agent disclosed by the invention not only improves the survival rate of the dendrobium officinale in a low-temperature environment, but also remarkably reduces the damage degree of the appearance of the dendrobium officinale compared with a control group without using the cold-resistant agent, which shows that the cold-resistant agent disclosed by the invention has a remarkable effect on protecting the dendrobium officinale from low-temperature damage. The cold-resistant agent disclosed by the invention is simple in preparation process, easily available in raw materials and relatively low in cost, so that the cold-resistant agent has a wide application prospect in the fields of large-scale planting and deep processing of dendrobium officinale.

The present invention relates to a composition containing Citrus sinensis (L.) Osbeck fruit and lemon extracts and preferably a trace element, which is preferably a mineral such as Chromium, Calcium, Magnesium, Iron, Copper, Zinc, Iodine, Manganese, Potassium, Sodium, Selenium, Molybdenum, Fluoride, Chloride or Phosphorus or combinations thereof, and their salts and / or a vitamin such as A, D, E, K, B1, B2, C, Niacin, Pantothenic acid, B6, Folic acid, B12 or Biotin or combinations thereof.

The invention discloses a graphene quantum dot material as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: S1, carrying out hydrothermal reaction on a mixed solution of mesoporous SiO2 and a carbon source to obtain a product I; s2, the product I and a fluoride solution are mixed to remove SiO2, and a product II is obtained; and S3, refining the product II to obtain the graphene quantum dot material. The carbon source is selected from at least one of triammonium citrate, diammonium hydrogen citrate, citric acid, folic acid, urea, thiourea and dicyanodiamine; the fluoride is selected from ammonium bifluoride and / or hydrogen fluoride. The particle size of the graphene quantum dot material provided by the invention is 15-30nm, the distribution is controllable, and the raw materials are easy to obtain; the prepared platinum nanoparticle / graphene quantum dot composite nanomaterial is high in solubility, stable in physical and chemical properties and remarkable in chemotherapy effect.

The invention relates to the technical field of pharmaceutical preparations, and particularly provides colon-targeted pH-responsive nano-liposome and application thereof in improving oral bioavailability of saponin. Aiming at the problems of gastric acid damage, small intestine metabolism inactivation and low colon absorption efficiency during oral delivery of saponin, a three-layer core-shell structure design is adopted, wherein an inner core is composed of a saponin-phospholipid crystal compound; the middle layer is a chitosan-PEG bimolecular layer, and pH response release is realized in a colon environment with pH of 7.4; and the outer layer is modified with a folic acid-PE conjugate to enhance colon cell targeted recognition. The uniform nanoparticles with the particle size of 80 + / -5 nm are prepared by combining a gradient pH drug loading method with a microfluidic focusing technology. According to the preparation, the saponin bioavailability (AUC0-12 reaches 43.31 h. Microgram / mL) is remarkably improved, and the colon targeting property (the cell uptake rate is 52.9%) is enhanced. The innovativeness is that the gastrointestinal segmented protection and accurate colon release are synergistically realized through a multi-layer functional design, and the solubilization-permeation paradox of the traditional delivery technology is broken through.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention discloses folate receptor mediated manganese ion coordination type cabazitaxel-loaded albumin nanoparticles as well as a preparation method and application thereof. Human serum albumin is covalently linked with folic acid through an amide condensation reaction to obtain a folic acid-albumin modifier with folic acid targeting; further combining with manganese ions through metal coordination to form a folic acid-albumin-manganese ion modifier with a targeting function; finally, efficient loading of cabazitaxel is achieved through a simple heating polymerization method, and the folate receptor mediated cabazitaxel-loaded albumin nanoparticles are formed. According to the nanoparticles provided by the invention, the particle size is about 140 nm, the stability is good, and the drug loading capacity and the encapsulation efficiency of cabazitaxel are high; the compound has a slow release effect, is good in in-vitro release behavior, and has important application value in the aspect of targeted delivery of drugs to tumors. Compared with other traditional nanoparticles, the nanoparticles show a remarkable anti-tumor effect.

The invention relates to the technical field of medical preparations, and particularly discloses a selenium-based anti-postmenopausal osteoporosis medicine and a preparation method and application thereof.The selenium-based anti-postmenopausal osteoporosis medicine comprises a main active component, a secondary active component and an auxiliary active component, the main active component is a nano-selenium-phytoestrogen compound and is formed by covalent coupling of selenomethionine and soy isoflavone, the particle size of the main active component is 50-200 nm, and the particle size of the auxiliary active component is 100-200 nm; the mass of the selenium element accounts for 5-30% of the mass of the compound; the auxiliary active component is a collagen peptide-calcium chelate and is prepared from marine collagen peptide and calcium citrate through chelation reaction, and the mass ratio of the calcium element to the collagen peptide is (1: 3)-(1: 5); the targeted delivery carrier is folic acid modified chitosan nanoparticles and is used for loading the main active component and the auxiliary active component; the auxiliary materials comprise a disintegrating agent, a lubricating agent and a pH-sensitive enteric coating material; through the synergistic effect of the main active ingredient nano-selenium-phytoestrogen compound and the auxiliary active ingredient collagen peptide-calcium chelate, bone formation can be promoted and bone resorption can be inhibited at the same time, and dual-function treatment is achieved.

The invention belongs to the field of food, and particularly relates to application of active folic acid substances to regulation of intestinal flora of a mother and a child. The invention provides an application of active folic acid substances in preparation of food which is taken by a mother and contributes to regulation of intestinal flora of the mother and offspring of the mother; wherein the active folic acid substances are selected from 6S-5-methyltetrahydrofolic acid and salts thereof, the mother is in a gestation period and / or a lactation period, and the offspring is in a fetal period, an infant period and / or a children period. The invention accidentally finds that the active folic acid substances are taken by the mother in the gestation period, so that the intestinal flora of the mother and the offspring can be obviously improved, and the active folic acid substances can promote the increase of beneficial bacteria in the intestinal tracts of the mother in the gestation period and the lactation period and the offspring of the mother and reduce the relative content of conditioned pathogens; furthermore, by adjusting the intestinal flora, the health of nervous systems of the mother and the offspring of the mother can be maintained through the intestinal brain axis.

The invention discloses a preparation method and application of a multifunctional layered nanosheet. The biomimetic hydrogel is prepared through a hydrothermal method, folic acid (FA) is modified on the surface of the biomimetic hydrogel, and the biomimetic hydrogel has the capacity of targeted recognition of pro-inflammatory phenotype macrophages, has the biomimetic activity of multiple enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and can effectively remove ROS; meanwhile, a succinate dehydrogenase (SDH) inhibitor dimethylmalonic acid (DMM) is loaded in an intercalation mode of the nano material, SDH activity is inhibited in a slow release mode, ROS outbreak caused by mitochondrial reverse electron transfer (RET) is blocked from the source, then a mitochondrial autophagy pathway mediated by BNIP3-LC3B is started, and dynamic and steady state recovery of mitochondria is achieved. The system finally realizes macrophage mitochondrial function reconstruction, oxidation environment improvement and transfer of healthy mitochondria to osteoblasts, so that the osteogenic differentiation capability is recovered, and the effect of treating osteoporosis is achieved.

The invention relates to the technical field of chemical synthesis of medicines, and particularly discloses a novel folic acid compound and application thereof, and the folic acid-containing compound has a structure as shown in a formula I or a formula II. The folic acid-containing compound can be used as a P-gp inhibitor, a chemotherapeutic drug sensitizer, a tumor metastasis inhibitor and a tumor multidrug resistance reversal agent. The folic acid-containing compound disclosed by the invention has good biological activity, excellent tumor multidrug resistance reversal activity and sensitization chemotherapy drug activity, the preparation method is simple, the yield is high, and the application prospect of folic acid and tetrahydroisoquinoline structures in the aspect of tumor multidrug resistance reversal agents is expanded. # imgabs0 #

The invention belongs to the field of biological medicine, and relates to a human serum albumin covalent binding type targeted folate receptor micromolecule drug conjugate prodrug and application thereof. The prodrug disclosed by the invention has a structure as shown in a formula I, six-carbon maleimide is used as a site combined with endogenous albumin, and albumin is used as a carrier to deliver the drug. The prodrug can increase accumulation of the drug in tumors under the EPR effect. A dipeptide chain composed of valine and alanine is used as a cleavage site of cathepsin B, an adapter composed of p-aminobenzyl ether and N-methylamino-4-nitrophenol releases drugs in an acidic environment, an azidohexanoic acid structure is added to a side chain of p-aminobenzyl ether, so that the site of a targeting ligand can be increased, and SN38 is used as a small molecule cytotoxic load. The micromolecular prodrug has dual functions of active targeting and passive targeting, and can effectively improve the concentration in solid tumors, reduce toxic and side effects and improve the anti-tumor effect under the mediation of related receptors.

The invention belongs to the technical field of biological medicines, and particularly relates to a nutritional composition as well as a preparation method, application and a product thereof. The nutritional composition provided by the invention consists of nicotinamide, B vitamins and a fungal polysaccharide compound, the vitamin B consists of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide compound is composed of a lucid ganoderma extract, a mushroom extract, an agaricus blazei murill extract, a hericium erinaceus extract, a coprinus comatus extract, a grifola frondosa extract and a cordyceps militaris extract. Western medicine source substances (nicotinamide and B vitamins) and traditional Chinese medicine source substances (fungal polysaccharide compounds) are combined, and the nutrition composition is prepared through combination of the Chinese medicine and the western medicine. The nutritional composition has remarkable effects of enhancing immunity, delaying senescence, resisting fatigue and resisting oxidation, and shows extremely wide application prospects in the field of preparation of health-care products, medicines and cosmetics.