78 results about "Trimethylglycine" patented technology

The application discloses a pH-responsive degradable composite bacteriostatic film derived from waste bone powder extract and a preparation method thereof. The main components of the film are collagen and hydroxyapatite extracted from waste bone powder, citric acid monohydrate-betaine and lactic acid-betaine eutectic solvent, and in addition, purple corn anthocyanin is used as a pH response agent, and citric acid monohydrate-betaine eutectic solvent is used as a crosslinking agent and a bacteriostatic agent. In the preparation process, collagen and hydroxyapatite are mixed with an aqueous solution of eutectic solvent and heated, and after the system is reduced to room temperature, the anthocyanin aqueous solution is added, mixed, and ultrasonic defoaming is performed, then the film forming liquid is poured into a clean mold, vacuum dried, and cooled to remove the film. The pH-responsive degradable composite film prepared based on the above method has good properties, not only has the characteristics that the overall color changes with the microenvironment pH, but also has good mechanical strength, bacteriostasis and degradability, and has wide application potential in many fields such as food preservation.

This invention relates to the field of cosmetic compositions, and more specifically to a synergistic anti-aging composition involving energy metabolism and autophagy, its preparation method, and its application. The composition comprises sea buckthorn fruit extract, trehalose, and a cosmetically acceptable polyol carrier. This invention pioneers a betaine-trehalose-NADES extraction system, achieving low-temperature, high-efficiency extraction while preserving the heat-sensitive active ingredients of sea buckthorn. Trehalose possesses both extraction and autophagy activation effects. A selective separation strategy achieves a balance between efficacy, compliance, and process simplicity. The green, circular process reduces costs and is environmentally friendly. Through the synergistic effect of sea buckthorn fruit extract and trehalose, the composition enhances mitochondrial membrane potential, increases ATP production, inhibits excessive activation of the mTOR pathway, and promotes LC3-II conversion, thus synergistically combating skin aging from multiple targets including cellular energy metabolism, signaling pathways, and autophagy.

This invention discloses a firming and wrinkle-reducing eye cream, comprising a moisturizer, a whitening agent, a skin repair additive composition, a thickener, a preservative, a film-forming agent, a skin moisturizer, and a surfactant. The weight ratio of the moisturizer, whitening agent, anti-aging additive composition, thickener, preservative, film-forming agent, skin moisturizer, and surfactant is 20:1:2:3:0.3:0.5:1:0.2. The moisturizer includes butylene glycol, glycerin, polydimethylsiloxane, and hydrogenated polyisobutylene. The skin-repairing additive composition includes hydroxypropyl tetrahydropyranotriol, adenosine, cell growth factors, ceramides, and salicylic acid, comprising olefins, betaine, trehalose, 1,2-hexanediol, hydrogenated lecithin, allantoin, and Euglena galactosides. The film-forming agents include polyacrylamide, cyclohexylsiloxane, and polydimethylsiloxane alcohol. The emollients include C13-14 isoparaffins, polydimethylsiloxane / vinyl dimethylsiloxane crosspolymers, and cyclopentamethoxysiloxane. This invention can repair cells and provide long-lasting anti-wrinkle and whitening protection for the corners of the eyes.

The present application relates to a kind of monodisperse core-shell type hybrid silica microspheres containing vinyl shell and its preparation and application. Specifically, using "two-phase method" strategy, monodisperse solid silica microspheres are used as matrix, vinyl triethoxysilane and tetraethyl orthosilicate are used as raw materials, lauryl amide propyl betaine and sodium subtiliporin are used as double-template pore-forming agent, under the catalysis of urea, sol-gel reaction occurs, a layer of porous hybrid shell containing vinyl functional group is constructed on the surface of solid silica microspheres, and core-shell type silica hybrid microspheres are formed. Because the shell contains a large number of vinyl functional groups, after hydrophilic modification with captopril, it is used as the adsorbent of hydrophilic interaction chromatography, and is applied to the selective enrichment of biological samples. The core-shell type silica hybrid microspheres prepared in the present application have uniform morphology, good monodispersity, and rich vinyl functional groups on the surface, which are easy to modify and apply subsequently.

This invention provides an oil-purifying cleansing mud and its preparation method. The cleansing mud, by weight, comprises 1-5 parts of a viscosity modifier, 0.01-0.1 parts of a chelating agent, 10-20 parts of a moisturizer, 15-25 parts of a surfactant, 0.1-1 parts of a thickener, 0.1-5 parts of a cleanser, 0.1-2 parts of an antioxidant, 0.1-1 parts of a fragrance, and 50-75 parts of water. The surfactant is a combination of sodium cocoyl glycinate, lauryl hydroxysulfonate betaine, cocoyl glutamic acid, decyl glucoside, potassium cocoyl glycinate, and cetearyl alcohol polyether-25. This oil-purifying cleansing mud, through the use of surfactants, cleansers, and moisturizers, gently cleanses pores, absorbs oil and dirt, and simultaneously soothes the skin, preventing dryness and tightness. The synergistic combination of these components achieves a balance between cleansing, oil removal, and gentleness, precisely regulating the skin's balance and thus enhancing the consumer experience.

This invention provides a green and efficient method for extracting and purifying lutein from marigolds, belonging to the field of extraction, separation, and purification of fat-soluble active ingredients. The invention involves pulverizing fresh or dried marigold flowers and then using an aqueous solution of dodecyl dimethyl betaine for green extraction and purification of lutein. Based on the characteristic that dodecyl dimethyl betaine forms supramolecular interfacial charges with lutein in aqueous solution, and utilizing the fact that low temperatures significantly affect the stability and morphology of micelles, the critical micelle concentration of dodecyl dimethyl betaine decreases with decreasing temperature, and the solubility of lutein in the mixed system further decreases, lutein is precipitated and aggregated. The lutein-bound state, such as lutein esters, is then saponified using an alcohol-alkali method, followed by ethanol recrystallization to obtain high-purity lutein. This new green and efficient method for extracting and purifying lutein from marigolds provides high extraction rates and high purity. The method not only improves the extraction efficiency of lutein but also has significant implications for the safe industrial production of lutein.

This invention discloses an EGCG supramolecular compound with antioxidant, liver-protective, and fat-reducing effects, its preparation method, and composition. The supramolecular compound is formed by non-covalent bonding between epigallocatechin gallate (EGCG) and ligand molecules containing trimethylamine and carboxyl groups; the supramolecular compound has a co-amorphous structure; the ligand molecules containing trimethylamine and carboxyl groups are betaine, ergothioneine, or L-carnitine. This invention can solve the problems of low stability and bioavailability of EGCG. It can significantly improve the solubility of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine, reduce the hygroscopicity of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine, and enhance the stability and bioavailability of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine.

The present application relates to the technical field of animal breeding, and discloses a compound nutritional health supplement for laying hens in the egg production period and a preparation and application thereof, which comprises the following raw materials in mass fractions: motherwort 1200-1400 parts, radix astragali 150-250 parts, radix codonopsis 80-120 parts, betaine hydrochloride 500-700 parts, butyric acid clostridium 50-70 parts, bacillus licheniformis 90-110 parts, vitamin acetate 3-5 parts, coated 25-hydroxy-cholecalciferol 1-2 parts, vitamin E acetate 15-25 parts, and rice husk powder 0-411 parts. The formula can scientifically and comprehensively regulate the digestion and absorption of nutrients, immune function and reproductive system health of laying hens, improve the eggshell quality, egg weight and nutritional quality of egg products, has stable effect and strong universality, greatly reduces the side effects and body burden caused by long-term medication while ensuring the effect, and is more suitable for the concept of healthy breeding; the health level of the body is comprehensively improved, the intestinal flora balance is maintained by probiotics, the immunity and stress resistance of laying hens are overall enhanced, the nutrient utilization rate is improved, and healthy high yield is achieved.

The application provides a high-efficiency detergent and a preparation method thereof, and relates to the technical field of daily chemical products.The detergent comprises microencapsulated composite enzymes, chelating agents, surfactants, additives and water; the active ingredients of the microencapsulated composite enzymes are composed of lipase, protease and amylase, and are prepared by sodium alginate-chitosan microencapsulation embedding, so that the enzyme activity is effectively protected, the stability of the enzymes in the detergent system is improved, and the targeted decomposition of composite stains is realized; the chelating agents are composed of tetrasodium iminodisuccinate and maleic acid-acrylic acid copolymer sodium salt, and can synergistically improve the hard water resistance and the stain removal efficiency; the surfactants are composed of sodium fatty acid methyl ester sulfonate, alkyl polyglycoside and cocamide propyl betaine, and have strong emulsifying and stain removing power and mildness.The detergent is suitable for cleaning tableware and fruits and vegetables, and has the characteristics of high stability, strong stain removing power, easy rinsing, mildness and environmental protection.

The application discloses a method for separating and extracting xylanase based on a deep eutectic solvent (DES) and a two-water-phase system, and belongs to the technical field of biological separation engineering and enzyme purification. The method comprises the following steps: first, preparing a deep eutectic solvent by mixing and heating betaine (Bet) and 1,2-propylene glycol (1,2-Prop); second, mixing the deep eutectic solvent, inorganic salt and water in a certain proportion to build a two-water-phase system, wherein the mass percentage of the deep eutectic solvent is 40%, and the dosage of the inorganic salt is 0.2 g / mL; and third, adding a hydrolyzate containing xylanase into the two-water-phase system to perform extraction separation. The two-water-phase system formed by the deep eutectic solvent and the inorganic salt is used to selectively distribute the xylanase, the extraction rate can reach more than 90%, and the enzyme activity retention rate is greater than 85%. Compared with a traditional polymer two-water-phase system, the method has the advantages of high separation efficiency, mild conditions, small enzyme activity loss, low cost, green environmental protection, simple operation and the like, and is suitable for industrialized separation and purification of xylanase.

The application provides a polypropylene dissolving agent and application thereof, and belongs to the technical field of olefin dissolving agents.The polypropylene dissolving agent comprises the following raw materials in mass fractions: dicyanamide salt ionic liquid 10-25 parts, betaine salt 5-15 parts, tetrafluoroborate salt ionic liquid 5-15 parts, ethyl sulfate salt ionic liquid 5-15 parts, acetate salt ionic liquid 10-20 parts, long carbon chain anion ionic liquid 10-40 parts, dispersant 2-18 parts and auxiliary agent 2-20 parts.Under the condition of 180-280 DEG C, the mass fraction of the polypropylene dissolving agent of the application to polypropylene with a molecular weight of 500-1000 million can reach more than 15%, and the wet processing of the ultra-high molecular weight polypropylene into a film can be realized, and the polypropylene diaphragm or polypropylene fiber obtained by processing has the characteristics of high temperature resistance, high strength (including tensile and puncture strength), high porosity, high air permeability and the like.

This invention discloses urinary metabolic biomarkers for hyperuricemia and their applications, relating to the field of metabolomics technology. It aims to overcome the shortcomings of traditional serum uric acid detection, such as susceptibility to interference, insufficient early warning capabilities, and a lack of sensitive, specific, and non-invasive biomarkers. The urinary metabolic biomarker is at least one of L-kynurenine, indole, and betaine, which can be used to prepare reagents or kits. The assessment system includes a sample analysis unit and a risk assessment model. This invention enables non-invasive and convenient detection of hyperuricemia, accurately captures early metabolic abnormalities, provides reliable risk stratification, and supports clinical auxiliary diagnosis and pathogenesis research.

Compositions, methods, and uses are contemplated in which a betalain-containing preparation is administered to a mammal to enhance athletic performance. Most preferably, the preparations are substantially nitrate free and acutely and transiently increase hematocrit, erythropoietin, adrenalin, and beta endorphin, while reducing serum lactate, serum lactate dehydrogenase, heart rate and a rate of perceived exertion.

This invention discloses a novel zwitterionic monomer and its preparation method and application in a hydrogel electrolyte. The zwitterionic monomer includes sulfobetaine urea methacrylate and sulfobetaine urea acrylate. The monomer N-acryloylglycine is copolymerized with the two novel zwitterionic monomers to form zwitterionic polymers. The zwitterionic polymer chains are linked by hydrogen bonds and covalent bonds to form a three-dimensional network. The metal salt is encapsulated in the three-dimensional network to obtain the zwitterionic hydrogel electrolyte. The zwitterionic hydrogel electrolyte exhibits low hysteresis, high conductivity, and maintains toughness and stretchability. The zwitterionic groups on the side chains of the zwitterionic polymer and the loaded metal salt give the elastomer excellent antifreeze and ion transport properties. Under low temperature conditions, the zwitterionic hydrogel electrolyte exhibits excellent mechanical flexibility and ion transport properties.

The application discloses a silk protein composition for hair growth, and belongs to the technical field of hair growth silk protein compositions. The composition comprises moisturizing agents, fragrance imparting agents, cationic surfactants, acidity adjusting agents, co-emulsifiers, preservatives, antioxidants, oily ingredients, trace ingredients, lauroylsarcosine sodium, cocoyl glutamic acid TEA salt, sodium lauroamphoacetate, sodium chloride, phenoxyethanol, sodium laurate, citric acid, allantoin, cocamide propyl betaine, hydroxybenzoic acid ester, nicotinamide and a silk protein solution. The application has the beneficial effect that the product contains proteins and amino acids, can supplement the required nutrients for damaged and fragile hair, can stimulate the cell proliferation of human head hair papilla cells and hair matrix cells, can make the head of people with thinning hair or baldness regenerate hair, and can prevent hair loss for people with normal hair.

The application discloses a mild cleaning composition and application thereof, and belongs to the technical field of cosmetics. The mild cleaning composition comprises sodium lauroyl glutamate, decyl glucoside, Tween-28, cocamidopropyl betaine, plant saponin and ikkduin. The plant saponin comprises camellia seed extract, and further comprises at least one of acanthopanax extract, soapberry extract and gynostemma extract. The mild cleaning composition is prepared by selecting suitable components, and the components have good interaction. When the mild cleaning composition is applied to the preparation of a washing and protecting product, the mild cleaning composition has good surface activity, can effectively clean the skin, is very mild, does not cause skin dryness and tightness after washing, does not affect the skin barrier function, and has low residue after use, thereby avoiding continuous stimulation to the skin.

This application relates to the field of food fermentation technology, and discloses a flavorful peptide-based low-salt fermented soybean paste and its production process. The production process includes: preparing a thermally saturated solvated zinc source precursor by using anhydrous betaine, zinc salt, and citrate; dispersing it in brine to prepare a fermentation broth; and inoculating with koji (fermentation starter) for variable-temperature fermentation. Fermentation is divided into three stages: a controlled-temperature initiation stage to establish the microbial culture; an enzymatic hydrolysis stage where the temperature is increased and the pH drop caused by acid production triggers zinc ion release, activating neutral proteases; and a post-ripening stage where anhydrous betaine is added to reduce water activity and construct an anti-corrosion barrier. This invention improves enzymatic hydrolysis efficiency through controlled zinc ion release and utilizes betaine to achieve low-salt preservation. The resulting product has a salt content ≤8.0g / 100g, an amino acid nitrogen content ≥0.8g / 100g, a rich umami flavor, and is stable during room temperature storage.

The application relates to a silicon-fluorine electrolyte waste gas treatment agent and a preparation method and an electrolyte waste gas treatment method, and belongs to the technical field of electrolyte waste gas treatment materials, and comprises 25-35 parts of a composite amine component, 35-45 parts of a functional alcohol ether component, 5-8 parts of a synergistic promoter, 2-4 parts of a stabilizer and 15-25 parts of deionized water, wherein the composite amine component is a mixture of N-methyldiethanolamine, N-(2-hydroxyethyl)ethylenediamine and triethanolamine, the functional alcohol ether component is a mixture of polyethylene glycol dimethyl ether and diethylene glycol butyl ether, the synergistic promoter is a mixture of beta-cyclodextrin and lauryl betaine, and the stabilizer is a mixture of sodium pyrophosphate and disodium ethylenediaminetetraacetate. The silicon-fluorine electrolyte waste gas treatment agent prepared by the application has high treatment efficiency and regeneration capacity.

This invention provides a method for preparing a thermosensitive gel for the prevention and treatment of nasal diseases and its application. By weight and volume percentage, it comprises recombinant human type III collagen, mussel adhesive protein, an anti-allergy agent, traditional Chinese medicine extracts, thermosensitive materials, and a sodium pyruvate-cocamidopropyl betaine ion-pair complex. This invention, by pre-forming the ion-pair complex, simultaneously solves the technical problems of existing nasal gels, such as poor anti-dilution ability, short adhesion time, and easy inactivation of active ingredients. The product is liquid below 30°C and can be sprayed for complete nasal coverage. At body temperature, it transforms into a gel within 2 minutes for long-lasting adhesion, exhibiting rapid anti-inflammatory, nasal patency-clearing, antipruritic, and mucosal repair effects. It is suitable for the prevention and treatment of allergic rhinitis and sinusitis, as well as nasal wound care, with significantly better efficacy than similar commercially available products.

This invention relates to the technical field of tobacco leaf preservation, and particularly to a tobacco humectant, its preparation method, and its application. The tobacco humectant is formed by a eutectic reaction of a hydrogen bond donor and a hydrogen bond acceptor. The hydrogen bond acceptor includes at least one selected from betaine, urea, diammonium hydrogen phosphate, ammonium chloride, and ammonium acetate. The hydrogen bond donor includes at least one selected from sodium phytate, tartaric acid, itaconic acid, potassium lactate, and D-glucopyranose. This invention, by forming a eutectic solvent system and constructing a stable hydrogen bond network, enables the prepared tobacco humectant to possess excellent dual functions of hygroscopic absorption and water retention. When sprayed onto reconstituted tobacco leaves, it solves the problems of rapid moisture release and easy drying of reconstituted tobacco leaves, significantly improving their water-holding capacity. Simultaneously, through precise selection of hydrogen bond donors and acceptors, it further reduces the impact on the user's smoking experience.

The application discloses a kind of special nutrient solution for container seedling of forest and preparation method thereof, belong to the technical field of forest seedling.The nutrient solution is based on macroelement, ethylenediaminetetraacetic acid chelated iron and conventional trace element, compound mineral source humic acid, compound amino acid, brown algae extract, paclobutrazol and compound rooting powder, add citric acid and mineral source potassium fulvate to stabilize EC value, prevent and control salt injury, and supplemented with chitosan and betaine to enhance seedling resistance, the pH value of nutrient solution is 5.5-6.5, EC value is 1.2-2.0 mS / cm.The application is prepared by mother liquor pretreatment, step-by-step dissolution, parameter control and membrane filtration process, can be segmented according to forest growth stage Application, can promote root seedling, control overgrowth, reduce salt injury, significantly improve seedling survival rate and seedling quality, suitable for large-scale production of container seedling of forest.

The application discloses a preparation method of dihydroquercetin self-assembled nanoparticles and application of the nanoparticles in liver protection, and the preparation method of the nanoparticles is as follows: betaine and dihydroquercetin are respectively dissolved in methanol; the methanol solutions of the betaine and the dihydroquercetin are mixed in a 1:1 molar ratio, and the pH is adjusted to 7.0-7.5 by using a sodium hydroxide solution; the mixture is added into preheated PBS under stirring, sealed and kept at 55-65 DEG C for 20-40 minutes; after being cooled to room temperature, the mixture is subjected to ultra-pure water dialysis by using a dialysis bag, and the BTNPs, i.e. dihydroquercetin self-assembled nanoparticles, are obtained after freeze-drying. The nanoparticles have good ALI treatment effect, high safety, can simultaneously solve the defects of low solubility and poor bioavailability of dihydroquercetin, and the defects of betaine, such as easy occurrence of gastrointestinal adverse reactions and existence of dose-dependent safety risks; meanwhile, the nanoparticles can also regulate intestinal flora disorder caused by alcohol and prevent endotoxemia.

This application relates to the field of personal care products, disclosing a no-rinse herbal skin-care bath liquid and its preparation method. The bath liquid raw materials include a herbal-eutectic precursor matrix, an aqueous solution of sodium cocoyl apple amino acid, deionized water, and a pH adjuster. The matrix is ​​made of betaine, matrine, dipotassium glycyrrhizate, and dimethyl isosorbide. The preparation method includes mixing the components and then adjusting the pH under negative pressure. During this process, the pH value and viscosity of the system are monitored in real time. When the instantaneous viscosity increases by a set proportion, the dropping rate of the acid adjuster is reduced and a high-speed shearing is initiated simultaneously. This invention utilizes the hydrogen bonding between specific matrix components to inhibit the crystallization and precipitation of herbal ingredients after water evaporation, improving the stickiness of residues. Simultaneously, by combining rheological feedback and negative pressure shearing technology, it alleviates the foaming and stratification problems of amino acid surfactant systems during the acid reduction adjustment process.

The application relates to the field of pet anti-aging and antioxidant medicines, in particular to a pet anti-aging and antioxidant special nutrient supplement and a preparation method thereof. The pet anti-aging and antioxidant special nutrient supplement comprises the following components in parts by weight: NMN 25-35 parts, manganese ascorbate 3-5 parts, coenzyme Q10 30-40 parts, hydrolyzed tannin 5-8 parts, Omega-3 fatty acid 180-220 parts, ergothioneine 2-3 parts, L-carnitine 20-40 parts, betaine 3-5 parts and vitamin E 5-8 parts. The various substances in the formula realize the pet anti-aging and antioxidant functions through a multi-pathway synergistic mechanism, which respectively are direct free radical scavenging, construction of an antioxidant defense network, repair of mitochondrial function, improvement of cell energy metabolism efficiency, blockage of inflammation and metabolic disorder and cutting off of an aging associated pathway.

This invention discloses a plant stress-resistance composition and its application, wherein the composition uses calcium chloride and / or betaine as active ingredients. After application, this plant stress-resistance composition can effectively alleviate high-temperature distortion in chrysanthemums, preventing the formation of ectopic bracts under high-temperature stress and reducing the loss of commercial value of cut flowers. Simultaneously, this composition has wide varietal adaptability, low raw material costs, and allows for flexible formulation adjustments for different chrysanthemum varieties, significantly improving economic efficiency compared to physical cooling methods.

This invention belongs to the field of personal care product technology, specifically relating to a male genital mousse wash containing PHMB and its preparation method. The wash uses polyhexamethylene biguanide hydrochloride (PHMB) as the core antibacterial agent, combined with cocoylpropyl betaine and sodium lauroyl sarcosinate as the cleansing surfactant system, compounded with sodium hyaluronate, panthenol, and dipotassium glycyrrhizate, supplemented with propylene glycol, glycerin, disodium EDTA, and phenoxyethanol. The pH is adjusted to 5.0–6.0 with L-lactic acid, and the remainder is water for injection. The preparation employs a low-temperature stepwise feeding process to obtain a weakly acidic mousse foam. The product exhibits an antibacterial rate of ≥99.99% against Escherichia coli, Staphylococcus aureus, and Candida albicans, is non-irritating to the skin, produces a dense and stable foam that is easy to rinse, and has good high and low temperature stability. It combines cleansing, antibacterial, and mucosal repair effects, solving the problems of traditional feminine hygiene products such as high irritation, easy allergy, and poor dosage form experience. It is suitable for daily cleaning and care of the male genitalia.

This invention discloses a fermentation method for L-lysine, belonging to the field of L-lysine fermentation technology. The fermentation method involves inoculating a secondary *Corynebacterium glutamicum* seed culture at an inoculation rate of 9.8-10.2% into a fermenter containing 1L of fermentation medium. The temperature inside the fermenter is maintained at 34-36℃, the pressure at 0.07-0.08 MPa, and the dissolved oxygen at 20-25%. The pH is maintained at 7-7.2 by adding ammonia. When the residual sugar decreases to 2g / L, a glucose aqueous solution is added to control the residual sugar to 5-8g / L. After 8 hours of fermentation, a betaine supplement is added. After 10 hours of fermentation, a culture medium supplement is added. This fermentation method can improve the L-lysine production rate, L-lysine yield, and sugar-acid conversion rate of *Corynebacterium glutamicum*.

The present invention relates to a cosmetic composition, particularly for hair care, comprising cationic inulins, anionic surfactants of the olefin sulfonate type, and amphoteric surfactants of the alkyl(amidoalkyl)betaine type. The invention also relates to a cosmetic treatment method, preferably for cleansing and / or washing keratinous materials, particularly hair, comprising a step of applying such a composition to said keratinous materials.

This invention discloses a method for in-situ preparation of Janus gel using a stepwise phase separation system. Based on an aqueous two-phase separation system formed by polyvinyl alcohol with sodium chondroitin sulfate A, dextran, polyethylene glycol, Pluronic F-127, Tween 80, 3-(N,N-dimethyldodecylammonium)propane sulfonate, or lauryl betaine in water, acrylic acid, a crosslinking agent, and a photoinitiator are added. After UV curing, freeze-thaw cycles, and solvent replacement by glycerol immersion, the corresponding Janus gel with a bilayer structure and high mechanical strength is obtained. This method for in-situ preparation of Janus gel based on an aqueous two-phase separation system can rapidly and conveniently prepare Janus gels with high mechanical strength and excellent asymmetric properties. Furthermore, the aqueous two-phase separation system has various combinations, allowing for the construction of various types of Janus gel patches, demonstrating good versatility.

The present application relates to a vaccine freeze-drying protective agent, its preparation method and application, and belongs to the technical field of biological medicine. The vaccine freeze-drying protective agent comprises the following raw materials: trehalose, mannitol, arginine, pullulan, 2-hydroxypropyl-beta-cyclodextrin, phospholipid, sodium sulfite and a protective additive; the protective additive is at least one of hydroxyectoin, betaine, dodecyl dimethyl betaine and polycarboxylic betaine. The preparation method comprises the following steps: mixing 2-hydroxypropyl-beta-cyclodextrin and phospholipid by high-speed shearing, sequentially adding the remaining components, adjusting pH and osmotic pressure, and then performing freeze-drying after sterile filtration. The protective agent can effectively inhibit phase separation, sugar recrystallization and interface adsorption during the freeze-drying process, significantly improve the activity retention rate and physical stability of the vaccine during freeze-drying and storage, and is suitable for the preparation of freeze-drying preparations of various vaccines such as viral vaccines, bacterial vaccines and recombinant protein vaccines.