241 results about "Oxidative damage" patented technology

The invention discloses a plant lactobacillus with light aging resistance and an application thereof. The preservation number of the plant lactobacillus cj001 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35419. The fermentation supernatant of the plant lactobacillus cj001 shows good anti-aging activity and in-vitro antioxidant activity, and the growth ability of HaCaT cells is promoted. After the combined fermentation product of the plant lactobacillus cj001 and the lactobacillus paracasei Grx701 is subjected to ultraviolet radiation, the proliferation of HaCaT cells can be promoted, the endogenous antioxidant capacity of human skin cells can be enhanced, the photooxidation damage can be effectively relieved, and the combined fermentation product has a wide application value in the field of preparation of products with antioxidant, anti-aging, anti-ultraviolet damage, anti-photoaging and the like.

The invention provides an edible coating fresh-keeping liquid suitable for low-temperature refrigeration of harvested tropical fruits and a storage and fresh-keeping method. The low-temperature cold storage edible film coating fresh-keeping liquid is soybean oil. When bananas or mangoes are coated with the edible coating liquid for low-temperature refrigeration, the occurrence of fruits under low-temperature refrigeration can be effectively inhibited, and the sensory quality and shelf life of the fruits can be maintained. Bananas or mangoes are subjected to soybean oil coating treatment, so that the relative conductivity of fruit tissues can be effectively reduced, and the integrity of fruit cell membranes is maintained; according to the present invention, the production of H2O2, O2 <-> and MDA can be significantly reduced, the activity of POD can be inhibited, and the activity of PPO, SOD and CAT can be enhanced so as to activate the anti-oxidation defense system of fruits, reduce the oxidative damage caused by cold injury, and enhance the accumulation of osmotic regulation substances; the content of antioxidant carotenoid can be increased, so that the stress resistance of the fruits is improved, and the cold injury of the fruits in the low-temperature storage process is reduced.

The invention discloses a lactobacillus mucus fermentation strain. The microbial preservation number of the lactobacillus mucus fermentation strain is CCTCC (China Center for Type Culture Collection) NO: M 20252262. The strain can inhibit enterotoxigenic escherichia coli, and can treat or slow down diarrhea, intestinal oxidative damage, intestinal inflammation damage, intestinal morphological damage, intestinal barrier function damage and intestinal flora disorder caused by the enterotoxigenic escherichia coli. The strain is a probiotic with a clinical application prospect.

The invention relates to the technical field of plant conditioners, in particular to application of nano silicon dioxide in relieving stress of polystyrene microplastics on hibiscus mutabilis. The invention finds that the influence of polystyrene micro-plastic on the stress of hibiscus mutabilis can be relieved to a certain extent during treatment by nano-silica, the intervention of the nano-silica causes the increase of secretion of melatonin, and the combined action of the nano-silica and melatonin makes the effect more remarkable and shows a synergistic effect. The nano silicon dioxide directly acts on the surfaces of cells to form a protective layer, so that adsorption and damage of PS-MPs to the cells are reduced, synthesis of melatonin is promoted, and a melatonin signal channel is activated. The activated melatonin signaling pathway further regulates the activity of carbon and nitrogen metabolism key enzymes, promotes carbon and nitrogen metabolism, improves synthesis and accumulation of carbohydrates and proteins, enhances the activity of an antioxidant system, removes active oxygen and reduces oxidative damage.

The invention discloses a compound microorganism freeze-drying protective agent, a preparation method of the compound microorganism freeze-drying protective agent and a use method of the compound microorganism freeze-drying protective agent. Comprising the following raw materials in parts by weight: 0.03 part of disodium ethylene diamine tetraacetate, 5 to 15 parts of trehalose, 2 to 5 parts of sorbitol, 0.5 to 2 parts of ascorbic acid, 0.05 to 0.2 part of N-acetyl-L-cysteine, 0.1 to 0.5 part of polylactic acid-glycolic acid copolymer, 0.01 to 0.05 part of alpha-tocopherol and 1 to 3 parts of bentonite. The PLGA, the alpha-tocopherol and the NAC construct a three-stage synergistic anti-oxidation system from outside to inside: the PLGA is externally blocked, so that rapid consumption of an antioxidant is avoided, and slow release is realized; the alpha-tocopherol realizes oxidative damage repair of cell membranes; nAC maintains the internal reduction environment of cells, reduces oxidative stress and can regenerate alpha-tocopherol at the same time, and reutilization of resources is achieved.

The invention relates to a Saccharomyces cerevisiae C16I09 strain which is preserved in the China Center for Type Culture Collection on May 21, 2025, and the preservation number of the Saccharomyces cerevisiae C16I09 strain is CCTCC (China Center for Type Culture Collection) No. M20251150. The strain disclosed by the invention has good oxidation resistance and can be applied to the fields of skin care products, health care products, anti-aging foods and the like. Especially, extracellular fermentation broth of the strain, namely a fermentation product or a fermentation extract of the strain, has excellent oxidation resistance, can scavenge free radicals and relieve oxidative damage, has the effects of protecting skin, resisting inflammation, delaying senescence and the like, and can be applied to the fields of skin care products, health care products, anti-aging foods and the like as a natural anti-aging agent.

The invention relates to the technical field of biology, and discloses a method and equipment for dynamically detecting damage caused by ROS, and the method comprises the following steps: A, mixing ROS with a DNA skeleton nanostructure, and carrying out oxidative damage reaction; step B, within a period of time after the oxidative damage reaction occurs, sampling the morphological characteristics of the reaction product at multiple time points, and observing the change process of the morphological characteristics; c, according to the change process, the degree of damage caused by ROS to the DNA skeleton nanostructure is judged, and equipment capable of achieving the method comprises an ROS generation module, the DNA skeleton nanostructure, an oxidative damage reaction module and a data analysis module. The method can solve the problem that two types of ROS are difficult to visually distinguish in the prior art, can be used as a general system for evaluating the ROS regulation effect and performance of a nano material, and provides a new insight and method for deeply understanding the effect of ROS in biology and medicine, especially prevention and treatment of cancer and senescence related diseases.

The invention discloses protein soft sweets based on an olive pomace extract as well as a preparation method and application of the protein soft sweets. The protein soft sweets mainly consist of the following components in parts by mass: 10-50 parts of the olive pomace extract, 10-80 parts of collagen, 10-40 parts of gelatin, 1-20 parts of inulin, 1-10 parts of glucose syrup, 1-5 parts of malic acid and 1-5 parts of sodium citrate. The protein soft sweets based on the olive pomace extract have olive flavor, are amber, are crystal clear, glossy and rich in olive flavor before being sprayed with powdered sugar, and are moderate in viscosity, not sticky to teeth and good in elasticity and resilience after being sprayed with powdered sugar; the olive pomace extract prepared by the preparation method disclosed by the invention also has relatively high oxidation resistance, shows very high scavenging ability in the aspects of DPPH free radical scavenging ability and ABTS < + > free radical scavenging ability, has the free radical scavenging ability of the olive pomace extract, is beneficial to preventing oxidative damage and maintaining health, and can be used as an antioxidant functional food.

The invention relates to a truffle composition as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. The invention provides a truffle composition which comprises a truffle extract, retinol and nonapeptide-1, and the mass ratio of the truffle extract to the retinol to the nonapeptide-1 is as follows: the truffle extract to the retinol to the nonapeptide-1 is (0.026-200): (0.005-4): 1. The truffle extract disclosed by the invention has the effects of resisting inflammation, resisting oxidation, tightening and resisting wrinkles, and autophagy and brightening, and the retinol has the effects of tightening and resisting wrinkles; the nonapeptide-1 serving as a rhythm peptide has a rhythm rejuvenation effect, and the three components are matched to form the truffle composition which can synergistically improve a retinol metabolic pathway, accurately regulate and control retinol metabolism to remove oxidative damage, regulate and control anti-aging genes and collagen genes of skin and synergistically realize an anti-aging effect.

The invention discloses application of ellagic acid in preparation of a medicine or a biological product for preventing or relieving ultraviolet radiation damage and aging, and belongs to the technical field of biological medicine and radiation protection. Experimental verification shows that ellagic acid with effective concentration can promote expression of antioxidant related genes, reduce expression of inflammation response genes and expression of apoptosis related genes, eliminate active oxygen and inhibit lipid peroxidation reaction, so that oxidative damage, inflammatory reaction and apoptosis caused by ultraviolet radiation are effectively relieved, and the anti-inflammatory effect of ellagic acid is improved. The stress tolerance of an organism is improved, and the aging health risk induced by ultraviolet radiation is relieved; the medicine or biological product can be applied before and / or after exposure of ultraviolet radiation, and is suitable for preventing or relieving oxidative damage and cell aging caused by ultraviolet radiation. The invention provides a new way for developing safe and effective ultraviolet radiation protection and anti-aging preparations.

The invention belongs to the technical field of probiotics and application of the probiotics, and particularly discloses a strain of lactobacillus fermentum 121 and a preparation method of the lactobacillus fermentum 121. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 8, 2025, and the preservation number is CGMCC No.35547. The lactobacillus mucilaginosus 121 has remarkable oxidation resistance, and can be subjected to biological fermentation to generate quercetin. The compound can be used for developing health-care food, cosmetics and oral beauty products for preventing and / or relieving skin oxidative damage and / or aging, and has a wide application prospect.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

This invention relates to the field of bioactive peptide preparation technology, and discloses a method for preparing and applying a bifunctional antioxidant and anti-inflammatory peptide based on porcine skin collagen. The bifunctional peptide has the amino acid sequence GPSGPPGEKGP. Through peptidomics and computer simulation screening, this invention identified five novel antioxidant peptides with good solubility, non-sensitizing properties, and non-toxicity. Using an AFB1-induced HuH7 cell oxidative damage model, in in vitro cell experiments, GP-11 was shown to significantly reduce ALT, AST activity, and ROS levels, enhance the activity of SOD, CAT, and GSH-Px antioxidant enzymes, and activate the Keap1-Nrf2 pathway. It also reduced the levels of inflammatory factors TNF-α and IL-6 by downregulating the classical cGAS-STING inflammatory pathway. GP-11 exhibits both significant antioxidant effects and the ability to reduce the levels of inflammatory factors, demonstrating a dual function of antioxidation and anti-inflammation.

The application belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to a fermented Lactobacillus muci genus with a function of delaying aging and application thereof. The bacterium was preserved in the Guangdong Microbial Culture Collection Center on August 27, 2025, and the preservation number is GDMCC No: 66877. Experimental results show that the bacterium has excellent antioxidant performance, can relieve oxidative damage, delay aging, inhibit the expression of aging-related genes, and has the ability to improve the memory reproduction ability decline caused by aging, improve the body's anti-inflammatory ability, improve the intestinal flora imbalance induced by aging, and can regulate the level of fat factors, improve energy metabolism, and has the potential of anti-fat deposition and protection of liver function. Therefore, the bacterium can be used to develop products with multiple beneficial functions such as delaying aging, helping to antioxidant, auxiliary improving memory, helping to regulate intestinal flora, and has the advantages of high safety, strong gastrointestinal tolerance, good colonization ability, and has good application value.

The present invention belongs to the field of biomedicine. Disclosed are a collagen peptide, a composition and the use thereof. Specifically disclosed are a type 21 recombinant humanized collagen peptide (ColpepA1 21) and a composition thereof, and the use of a collagen material in anti-aging treatments, skin care and wound repair. The recombinant collagen of the present invention can effectively promote the repair process after skin injury, enhance the hydration state and barrier function of skin, reduce fine lines and wrinkles, improve the firmness and elasticity of skin, thereby significantly promoting key biological reactions in the wound healing process. Further disclosed in the present invention is a composite collagen material, which can effectively promote wound healing and cell regeneration, and alleviate oxidative damage.

The invention relates to the technical field of medicines for treating intestinal ischemia-reperfusion injury, and discloses a medicine for treating intestinal ischemia-reperfusion injury and a preparation method thereof. Comprising 10 to 25 parts of rosmarinic acid, 5 to 15 parts of betaine, 3 to 10 parts of glutamine, 10 to 25 parts of salvianolic acid B, 5 to 15 parts of astragaloside, 3 to 10 parts of ginsenoside Rg1, 2 to 8 parts of curcumin, 5 to 12 parts of ophiopogon japonicus polysaccharide, 2 to 6 parts of notoginsenoside R1, 3 to 7 parts of total flavonoids of oldenlandia diffusa and 4 to 9 parts of angelica sinensis polysaccharide. The medicine disclosed by the invention can be used for relieving intestinal edema, repairing oxidative injury, clearing excessive ROS, improving SOD activity, blocking oxidative stress injury, inhibiting epithelial cell apoptosis, reducing intestinal permeability and maintaining mucous membrane integrity, and has an excellent treatment effect on intestinal ischemia reperfusion injury.

The invention discloses asterias amurensis glucan as well as a preparation method and application thereof, and belongs to the technical field of biological medicine, the asterias amurensis glucan is obtained by combining an enzymolysis method with anion exchange column chromatography and gel column chromatography purification, the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose, and the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose. A terminal alpha-D-glucose branch chain is arranged at the O-6 position of a main chain, and meanwhile, a small amount of mannose and galactose are contained. Moreover, it is proved for the first time that the asterias amurensis glucan can serve as an intestinal barrier protective agent, and oxidative damage, caused by H2O2, of intestinal epithelial cells is improved by up-regulating an Nrf2 signal channel and tight junction protein. Therefore, the asterias amurensis glucan prepared by the invention has a huge potential of being developed into medicines and health-care products for protecting intestinal tracts.

The invention discloses a method for synergistically improving the activity of porcine oocytes through low-temperature plasma and erythritol, and belongs to the technical field of reproductive biology. The method comprises the following steps: collecting pig ovaries and separating oocytes; freezing protection is carried out by adopting a freezing liquid (DMSO: EG: FSF = 2: 2: 6) containing 0.5 mol / L erythritol, and preservation is carried out at-80 DEG C for 48 hours; and after unfreezing, treating for 30 seconds by using low-temperature plasma (6.83 kHz, 4.8 kV) to activate the cells. Experiments show that the erythritol can reduce frozen ice crystal damage, and the plasma treatment can significantly improve the cell activity after resuscitation, reduce the ROS content, increase the GSH reserve and enhance the mitochondrial membrane potential. And the survival rate of the oocytes is improved through synergism of the two. The problems that the cell activity is reduced and oxidative damage is serious in the cryopreservation process are solved, and an efficient technical scheme is provided for preservation of pig germplasm resources.

The invention discloses a method for enhancing plant salt stress resistance based on a polyoxometallate-rhizosphere bacterium composite system. The method comprises the following steps: step 1, preparing a polyoxometallate nanocluster with enzyme-like activity; step 2, carrying out surface modification on the polyoxometallate by adopting polyethyleneimine; step 3, loading the modified polyoxometallate on the surfaces of rhizosphere microorganisms to construct a polyoxometallate-rhizosphere bacterium composite system; 4, applying the composite system to plants under the salt stress condition so as to relieve salt stress and promote plant growth. The method has the advantages that the method for regulating and controlling the salt stress resistance of the plants is simple and convenient to operate and high in practicability; by synergistically exerting the effects of removing active oxygen and promoting growth of rhizosphere microorganisms of the polyoxometallate, oxidative damage induced by salt stress is effectively reduced; the method is good in biocompatibility and suitable for plant growth regulation and control under the salt stress condition.

The application provides an optimized lactoferricin peptide, a preparation method thereof and application thereof in preparation of anti-inflammatory and anti-oxidative damage preparations, and belongs to the technical field of biological medicines.The application obtains high-biological-activity lactoferricin peptide by optimizing amino acid sites of lactoferricin B; compared with the original lactoferricin B, the optimized lactoferricin peptide has better anti-inflammatory and anti-oxidative effects, can effectively reduce alcohol-induced oxidative damage, fatty degeneration and apoptosis of liver cells, and promote recovery of liver function.The lactoferricin peptide provided by the application can reduce the elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels by about 25% and 20%, respectively.The application provides an application scheme in development of a new generation of polypeptide drugs for treating alcoholic liver disease, hepatitis, acute lung injury and various inflammation oxidative stress related diseases.

The invention provides a composition with oil-controlling and pore-shrinking effects and application thereof. The composition with the oil-controlling and pore-shrinking effects comprises a phellinus linteus extract and a litchi peel extract. The phellinus igniarius extract and the litchi peel extract in the composition are matched for use, have a synergistic effect, can inhibit sebaceous gland cell grease secretion and reduce grease oxidation through an oil control channel, and repair cell oxidative damage caused by grease secretion and grease oxidation. And inflammatory reaction caused by sebum oxidation and flora imbalance can be relieved, so that the effects of controlling oil and shrinking pores are remarkably realized.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The invention belongs to the technical field of protein engineering, particularly relates to a protein tag with superoxide dismutase activity and application, and provides a protein tag which comprises polypeptide or protein with superoxide dismutase activity. On the second aspect, a fusion protein is provided and comprises the protein tag described in the first aspect and at least one target polypeptide or protein, the target polypeptide or protein is not superoxide dismutase, and the target polypeptide or protein is not superoxide dismutase only by using the protein tag disclosed by the invention. According to the present invention, the technical effects of target polypeptide or protein expression quantity detection (SOD enzyme activity detection), target polypeptide or protein protection from oxidative damage, target polypeptide or protein stability improvement and target polypeptide or protein solubility improvement can be simultaneously achieved without adding any additional step compared with the fusion protein expression operation in the prior art; the operation is convenient.

The invention relates to application of a reagent for inhibiting STAT3 gene expression in treatment of oxidative damage of trabecular meshwork cells. According to the invention, a signal channel STAT3 to EIF4E to TGF-beta-SMAD < 2 / 3 > for regulating and controlling the oxidative damage of the trabecular meshwork cells is innovatively found, and the STAT3 activates the TGF-beta-SMAD < 2 / 3 > channel by combining with EIF4E3, so that the apoptosis, oxidative stress and fibrosis of the trabecular meshwork cells caused by the oxidative damage are promoted. After the expression of the STAT3 gene in trabecular meshwork cells is inhibited, the EIF4E3 level can be reduced, and the apoptosis, oxidative stress and fibrosis of the trabecular meshwork cells induced by EIF4E3 are improved. Therefore, a reagent for inhibiting STAT3 gene expression can be used for treating trabecular meshwork cell oxidative damage and eye diseases related to the trabecular meshwork cell oxidative damage, including glaucoma and the like.

The invention discloses a traditional Chinese medicine composition for inhibiting salmonella choleraesuis as well as a preparation and application thereof, and belongs to the technical field of traditional Chinese medicine and network pharmacology. According to the invention, traditional Chinese medicines with a good antibacterial effect on S. Choletaesuis are screened, and the compound five-plum decoction with a good antibacterial effect on S. Choletaesuis is established. The five-plum decoction can be used for effectively regulating and controlling the steady state of intestinal flora, and reducing the oxidative damage and inflammatory damage of the S. Cholera to the intestinal tract and the liver by regulating and controlling the signal channels of TLR4 / NF-kB / MyD88 and ZO-1 / Claudin-1 / Occludin. Through the multiple effects of resisting bacteria, resisting inflammation, protecting intestinal barriers and improving production performance, the five-plum decoction shows remarkable prevention and treatment effects on swine paratyphoid, and has huge value of being developed into a novel veterinary traditional Chinese medicine preparation and put into clinical application.

The present application discloses a plant lactobacillus CCFM1366 capable of transforming various pueraria flavones and having the effects of alcoholism relieving and liver protecting, and a postbiotic prepared from the plant lactobacillus CCFM1366, and belongs to the technical field of microorganisms. The plant lactobacillus CCFM1366 and the postbiotic prepared from the plant lactobacillus CCFM1366 have at least one of the following effects: (1) improving alcohol metabolism enzyme activity and enhancing alcohol metabolism; (2) improving liver steatosis, lipid accumulation, oxidative stress and inflammatory response caused by long-term alcohol exposure; (3) reducing ROS synthesis and thereby reducing oxidative damage to liver cells, promoting the synthesis of antioxidants and thereby improving the protective effect on liver cells; and (4) reducing inflammatory response. The plant lactobacillus CCFM1366 and the postbiotic prepared from the plant lactobacillus CCFM1366 can be used for preparing functional food and / or dietary supplements with the potential of alcoholism relieving and liver protecting, and have great application prospect.

The invention discloses a polypeptide with antioxidant activity and application thereof, and belongs to the technical field of biological medicine and fine chemical engineering. The amino acid sequence of the polypeptide is selected from QY, YG, YYYY, QPYY or WHYHDYKY. HPLC and MS structure confirmation shows that the synthesized polypeptide is high in purity and accurate in structure. In-vitro chemical experiments prove that the polypeptide (especially WHYHDYKY) has extremely high ABTS and DPPH free radical scavenging capacity, and the activity of the polypeptide is superior to or equal to that of glutathione (GSH). Cell experiments show that part of polypeptides (such as QY, YG, YYYY and QPYY) can significantly improve the survival rate of HepG2 cells under an oxidative damage model, and have a significant cell protection function. The polypeptide can be applied to the fields of antioxidant drugs, functional food, cosmetics, industrial antioxidants and the like according to the characteristics of the polypeptide.

The invention relates to the field of plant biotechnology and molecular biology, in particular to application of a VvECT10 gene in regulation and control of drought resistance of grapes. A strain with overexpressed VvECT10 gene is slow in leaf water loss and light in withering degree under a drought condition, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) is remarkably reduced, and the activity of peroxidase (POD) and catalase (CAT) is remarkably improved, which indicates that the overexpression of the VvECT10 gene can enhance the drought resistance of the grape plant and relieve oxidative damage.

This invention discloses a skin protection composition containing natural antioxidant active substances and its preparation method, relating to the field of daily chemical products technology. The composition includes natural antioxidant active components, an oil phase, an aqueous phase, a moisturizing component, a thickening and stabilizing component, and auxiliary components. This invention enhances skin protection by using maqui berry extract as the core antioxidant ingredient and sea buckthorn flavonoid extract. By employing elderberry extract as an antioxidant compound ingredient, it complements maqui berry extract and sea buckthorn flavonoids, creating a multi-target synergistic effect in the antioxidant mechanism. This constructs a multi-target synergistic antioxidant defense network, achieving complementary and synergistic effects in the mechanism of action. It blocks the oxidation chain reaction at multiple stages, and the compounding of various natural antioxidant active substances provides more comprehensive and longer-lasting protection for the skin from oxidative damage, achieving highly effective skin protection.

This invention belongs to the field of antitumor drug delivery technology, and discloses a dual-drug sequential delivery system, a composite hydrogel, its preparation method, and its applications. This invention achieves sequential release by encapsulating a cobalt (Co)-polyphenol coordination nanozyme (CoNZ) in a hydrogel and integrating it with microfibrils loaded with chloroquine (CQ). This invention provides a promising new approach to improving the treatment of CRPC by initially inducing ROS-mediated oxidative damage, subsequently inhibiting autophagy and blocking the TLR9 / NF-κB signaling pathway, synergistically disrupting the survival mechanisms of tumor cells.