828 results about "Oxidative damage" patented technology

The invention relates to a method for screening and preparing a horse milk whey protein source antioxidant peptide and application of the horse milk whey protein source antioxidant peptide. The polypeptide with potential antioxidant activity is screened out by predicting information of the polypeptide through virtual enzymolysis and a computer and then performing molecular docking with the keap1 protein. The feasibility of obtaining the antioxidant peptide is verified by simulating gastrointestinal digestion with pepsin, trypsin and chymotrypsin. Finally, in-vitro chemical antioxidant activity verification is carried out through chemical synthesis of the high-purity antioxidant peptide, and it is found that the antioxidant peptide has good free radical scavenging activity. An oxidative damage model is induced by H2O2 on cells, and it is found that the antioxidant peptide can resist cell oxidative damage by exciting the activity of a series of antioxidant enzymes. The natural antioxidant peptide screened by the invention has wide application prospects in the fields of foods, medicines, cosmetics and the like, and an efficient way is provided for development of horse milk whey protein source functional polypeptide products.

The invention relates to the field of biomedical materials, in particular to a novel composite collagen material for wound repair, and particularly relates to a preparation method of the novel composite collagen material containing 21-type recombinant humanized collagen (ColpepA1 21) and application of the novel composite collagen material in wound repair. The ColpepA1 21 is used for remarkably promoting key biological reactions in the wound healing process, including accelerating cell migration, enhancing the skin hydration state and barrier function and improving the stability and elasticity of the skin structure, a novel composite collagen material is developed, wound healing and cell regeneration can be effectively promoted, and oxidative damage can be relieved.

The invention discloses an application of soluble lignin as a synergist for ethanol fermentation and / or improvement of stress resistance of yeast under a fermentation environment stress condition, the soluble lignin is added into an ethanol fermentation system, and the problems of pH instability, oxidative damage, heavy metal inhibition and the like in ethanol fermentation can be synchronously solved; the survival rate of yeast and the yield of ethanol are remarkably improved, the process steps are simplified, the cost is reduced, the method is suitable for ethanol industrial production of agricultural and forestry wastes and other raw materials, and a green and practical technical path is provided for efficient utilization of all components of lignocellulose. In addition, the soluble lignin disclosed by the invention can also be recycled, can still keep a relatively high synergistic effect after being recycled for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

The invention discloses a compound composition with a multi-target heart protection function as well as a preparation method and application of the compound composition. The composition is composed of a basic functional oil phase, a core antioxidant / anti-inflammatory phase, a myocardial nutrition and regulation phase and a bioavailability promotion phase in a synergistic manner, and is integrated into a composite cardiac oligopeptide and microencapsulated particle key component substance through a specific enzymolysis and microencapsulation embedding process system. The invention aims to solve the technical problems of single target spot, insufficient synergistic effect of all components, low bioavailability of active components and poor stability of the existing heart protection product, and provides a stable and easily-absorbed composite composition which can realize synergistic effect from multiple channels at the same time. Technical effect verification shows that under the condition of equal-dose sample feeding, the composition can remarkably improve the bioavailability of functional components and shows excellent myocardial cell oxidative damage resistance and myocardial ischemia improvement activity in cell and animal models, the effect is remarkably superior to that of independent use or simple physical mixing of all the components, and the composition is suitable for clinical application. And the substantial progress of synergistic interaction is proved. The composition can be used for developing various medicines, health foods and functional foods beneficial to heart health.

The invention is suitable for the technical field of molecular biology, and provides an application of an SlNAC61 gene and a protein coded by the SlNAC61 gene in regulating and controlling the salt tolerance of tomatoes. According to the invention, an SlNAC61 overexpression vector is successfully constructed and is introduced into a tomato plant, and an SlNAC61 overexpression strain is screened out. Under the condition of salt stress, the oxidative damage degree of leaves of an SlNAC61 overexpression plant is relatively light, which suggests that the SlNAC61 gene may influence the salt tolerance of the plant by regulating the accumulation of ROS in the plant. Besides, after long-time salt stress treatment, the wilting degree of the SlNAC61 overexpression strain is relatively light, and the reduction ratio of the plant height, stem diameter, fresh root weight and dry root weight is lower than that of a wild plant MT. The result shows that overexpression of the SlNAC61 gene can enhance the salt tolerance of the tomato plant, and an important theoretical basis and a practical basis are provided for subsequently cultivating a salt-tolerant tomato variety by using a gene engineering means.

The invention relates to the technical field of health care product preparation, and particularly discloses an oyster peptide enzyme oral liquid capable of enhancing immunity and a preparation method thereof, the oyster peptide enzyme oral liquid comprises the following components: 20 parts of oyster peptide, 50 parts of plant enzyme liquid, 5 parts of honey, 2 parts of fructo-oligosaccharide, 2 parts of lemon juice and 100 parts of purified water; the oyster peptide enzyme oral liquid contains rich natural antioxidant ingredients including oyster peptide and plant enzyme liquid, the oyster peptide contains a large amount of sulfur-containing amino acid, and the ingredients can be used as antioxidants to reduce generation of free radicals and protect cells from oxidative damage; the plant enzyme is rich in natural antioxidant enzyme, can promote synthesis of antioxidant enzyme in vivo and remove free radicals in vivo so as to slow down senescence and diseases, the oyster peptide and the enzyme act together to improve the activity of catalase in vivo, and oxidative stress is reduced by removing superoxide anion free radicals.

The invention relates to the field of tea fermentation, in particular to a preparation method and application of black tea fermentation liquor with an anti-aging effect. The black tea fermentation liquor rich in tea polyphenol, thearubigins, theaflavins and theabrownin is prepared by jointly fermenting black tea with pichia kudriavzevii and bacilli. The black tea fermentation liquor has the functions of regulating cell metabolism and functions, repairing oxidative damage of cells, keeping completeness of mitochondrial functions, promoting synthesis of a high-energy phosphate compound ATP, enhancing cell activity and resisting aging, and can be applied to food, cosmetics and health care products. The fermentation process provided by the invention is green, safe and simple, and has practical application significance.

The invention relates to preparation of high-stability mascarpone cheese and application of the mascarpone cheese in baking, and belongs to the technical field of cheese preparation. The glutamine transaminase is combined with a citric acid-sodium citrate system, so that the excessive denaturation of protein is reduced, and the texture and flavor of the cheese are improved. Trehalose and xanthan gum are added for locking water and resisting freezing, and an antioxidant is combined for use, so that the freeze-thaw stability and oxidation resistance of the cheese are improved, the cheese is protected from texture change and oxidative damage in the freezing and unfreezing processes, and excellent texture and flavor can still be kept after multiple times of circulation. Arabic gum is modified through octenyl succinic anhydride, amphipathy is obtained, whey separation is prevented, whey loss is reduced, and the cheese taste is improved.

The invention relates to a method for improving cadmium stress resistance of rice by using carbon quantum dots (CDs), which solves the technical problem that rice growth is affected due to the fact that rice is deeply poisoned by cadmium in the prior art by spraying the carbon quantum dots on leaf surfaces of the rice or irrigating the carbon quantum dots into root soil of the rice. CDs are used as a leaf surface resistance and control agent to treat cadmium-stressed rice, and it is found that on the premise that growth and development of the rice are not affected, CDs can improve the stress resistance of the rice by promoting photosynthesis of the rice and can stimulate a rice defense system to generate more antioxidant enzymes to cope with oxidative damage caused by Cd stress. Besides, CDs can also reduce the concentration of DTPA-Cd in soil and reduce accumulation of Cd on the overground part so as to further reduce the toxicity of Cd to rice, and meanwhile, the Cd content of the overground part of the rice is reduced so as to be beneficial to reducing accumulation of Cd in grains, so that CDs can be used as a leaf surface resistance and control agent to effectively relieve cadmium toxic rice, thereby promoting growth and development of the rice and improving the yield.

The present invention relates to the field of biomedical materials, particularly to a novel composite collagen material for wound repair, and more specifically to a method for preparing a novel composite collagen material comprising recombinant humanized collagen type 21 (ColpepA1 21) and its use in wound repair. The present invention utilizes ColpepA1 21 to significantly promote key biological reactions in the wound healing process, including accelerating cell migration, enhancing skin hydration and barrier function, and improving the stability and elasticity of skin structure. The resulting novel composite collagen material effectively promotes wound healing and cell regeneration, alleviating oxidative damage.

The present disclosure provides for improved compositions of ionizable lipid nanoparticles for the delivery of therapeutic nucleic acids to cells. Anionic phospholipids, including phosphatidyl serine and phosphatidylglycerol are included in the lipid nanoparticles to increase the transfection efficiency in human dendritic cells. The further incorporation of mono-unsaturated alkyl chain analogs in dimethylaminopropyl-di oxolane or heterocyclic ketal ionizable lipids in the formulation demonstrated high levels of transfection in human dendritic cells, compared to other ionizable lipids in the same family, and demonstrated good stability to oxidative damage. Finally, the use of an ammonium salt of phosphatidylserine allows for the efficient production of PS-targeted LNPs.

The invention relates to the field of food and health care products, and discloses a composition for dispelling effects of alcohol and protecting liver, which comprises 20-100 parts of ampelopsis grossedentata leaf extract; 50 to 200 parts of a radix puerariae extract; 10-50 parts of a semen hoveniae extract; 10-50 parts of a turmeric extract; 10 to 100 parts of a yeast extract; 5-50 parts of a licorice extract; and 5 to 20 parts of pyrroloquinoline quinone disodium salt. The ampelopsis grossedentata leaf extract, the kudzuvine root extract and the hovenia dulcis thunb extract are utilized, alcohol decomposition is promoted, the blood ethanol concentration is reduced, and drunkenness symptoms are relieved. Puerarin improves activity of liver metabolic enzymes, accelerates discharge of ethanol and acetaldehyde, and reduces liver damage. The anti-oxidation effects of dihydromyricetin and PQQ can eliminate alcohol metabolism free radicals and protect liver cells. The components such as puerarin, hovenia dulcis thunb, curcumin and glycyrrhizic acid jointly promote liver cell regeneration, inflammation and oxidative damage are reduced, the levels of ALT and AST in serum are reduced, and liver health is maintained.

The invention relates to the field of modified milk, in particular to pasteurized modified milk with a lead ion emission reduction function and application of the pasteurized modified milk. The pasteurized modified milk provided by the invention contains lactobacillus mucus fermentation B44, wherein the preservation number of the lactobacillus mucus fermentation B44 is CGMCC (China General Microbiological Culture Collection Center) No.17321. Compared with a control sample and other strains, the lactobacillus mucilaginosus B44 in the pasteurized modified milk provided by the invention can remarkably reduce the lead content in intestinal content supernatant, and results of in-vivo experiments of rats show that the lactobacillus mucilaginosus B44 can remarkably reduce the blood lead concentration, the lead content in livers and the lead content in kidneys of drinking objects, so that the lactobacillus mucilaginosus B44 can be used for preparing the pasteurized modified milk. The discharge of lead in excrement can be increased, and liver oxidative damage caused by lead poisoning can be effectively relieved, so that the method can be applied to research on in-vivo emission reduction of lead.

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention relates to a plant lactobacillus with effects of dispelling effects of alcohol, protecting the liver and restoring consciousness and application of the plant lactobacillus. The plant lactobacillus is a plant lactobacillus BGI-J9 strain, and the preservation number of the plant lactobacillus is CCTCC NO: M 20242630. The invention further discloses a preparation method of the plant lactobacillus with the effects of dispelling effects of alcohol, protecting the liver and restoring consciousness and application of the plant lactobacillus with the effects of dispelling effects of alcohol, protecting the liver and restoring consciousness. The strain has excellent tolerance to gastric acid, intestinal juice and cholate in a gastrointestinal tract system, can survive in the gastrointestinal tract, and provides a basis for continuously playing a probiotic effect; the compound has excellent inhibition capability on common intestinal pathogens; free radicals can be removed, and oxidative damage to the body is reduced; good alcohol metabolism enzyme synthesis capability and alcohol metabolism promoting potential are realized; the strain also has an excellent improvement effect on alcohol-damaged mice, specifically, the metabolism of ethanol and acetaldehyde in a body is accelerated, the occurrence time of drunkenness is obviously delayed, and the sobering time is shortened; intestinal injury and systemic inflammation caused by alcohol are improved; liver oxidative stress caused by alcohol is improved, and the occurrence and development process of alcoholic fatty liver is delayed; and cognitive function impairment caused by alcohol is improved.

The invention discloses a method for improving in-vivo and in-vitro antioxidant activity of pomegranate peel polyphenol extract through combined enzymolysis of tannase and beta-glucosidase, and relates to the technical field of food science and biology. The pomegranate peel polyphenol extract provided by the invention is obtained from pomegranate peel powder through solvent extraction. According to the present invention, tannase and beta-glucosidase are adopted to perform single or combined enzymolysis on the pomegranate peel polyphenol extract, and the determination on the in vitro antioxidant activity of the pomegranate peel polyphenol extract shows that the antioxidant activities of DPPH and ABTS after the combined enzymolysis are improved by 29.84% and 29.30% compared with the antioxidant activities of the untreated polyphenol extract; the UPLC-ESI-QTOF-MS is used for identifying and analyzing the components of the enzymatic hydrolysate, the content of macromolecular ellagic tannin is obviously reduced, and the content of micromolecular ellagic acid and gallic acid is increased. The enzymolysis polyphenol is applied to D-galactose induced oxidative stress mice, and the result shows that the oxidative damage relieving effect of the enzymolysis polyphenol on the mice is better than that of untreated polyphenol.

The present disclosure provides for improved compositions of ionizable lipid nanoparticles for the delivery of therapeutic nucleic acids to cells. Anionic phospholipids, including phosphatidylserine and phosphatidylglycerol are included in the lipid nanoparticles to increase the transfection efficiency in human dendritic cells. The further incorporation of mono-unsaturated alkyl chain analogs in dimethylaminopropyl-dioxolane or heterocyclic ketal ionizable lipids in the formulation demonstrated high levels of transfection in human dendritic cells, compared to other ionizable lipids in the same family, and demonstrated good stability to oxidative damage. Finally, the use of an ammonium salt of phosphatidylserine allows for the efficient production of PS-targeted LNPs.

The invention belongs to the technical field of bioactive peptides, and particularly relates to an antioxidant polypeptide as well as a preparation method and application thereof. The antioxidant polypeptide provided by the invention contains at least one of LMGPR, GFDGD, FGFDGDF and GHNGLDGL. The antioxidant polypeptide has better oxidation resistance, can relieve oxidative damage and inflammation, plays a role in protecting oxidative stress cells, is non-toxic to cells, and can be used as an active component of an antioxidant product. The four peptide fragments also have a synergistic effect in the anti-oxidation aspect, and an excellent anti-oxidation effect can be generated by compounding use of the four peptide fragments.

The invention relates to armadillidiam vulgare derived carbon quantum dots as well as a preparation method and application thereof, the armadillidiam vulgare derived carbon quantum dots are characterized in that the particle size of the armadillidiam vulgare derived carbon quantum dots is 2.2-6.0 nm, and the armadillidiam vulgare derived carbon quantum dots can inhibit pain response, reduce oxidative stress level and reduce inflammatory factor gene expression level. The armadillidiam vulgare derived analgesic carbon quantum dots inhibit irritant movement and oxidative damage in a pain model, and reduce inflammatory factors and pain pathway gene expression; the oxidative stress level is reduced, and the effects of relieving pain, resisting inflammation and resisting oxidative stress are achieved.

The invention provides a method for reducing cadmium content in rice under cadmium stress, a rice adjusting method and application. By adding the exogenous water-soluble organic selenium into the cadmium-polluted farmland soil, the exogenous water-soluble organic selenium is proved to have the effects of promoting the growth of the rice under the cadmium stress and relieving the cadmium stress of the rice; the exogenous water-soluble organic selenium is beneficial for improving the activity of antioxidant enzymes of the rice and reducing oxidative damage, and has a positive effect on relieving cadmium toxicity of the rice; in addition, the exogenous water-soluble organic selenium is beneficial to reducing direct migration of cadmium from rice roots to brown rice and effectively reducing the biological enrichment coefficient of Cd in the brown rice; the exogenous water-soluble organic selenium can promote generation of iron plaque on the root surface of the rice in the mature period and form an effective barrier for Cd absorption of the rice, so that accumulation of cadmium in rice plants is reduced, and toxicity of cadmium to the rice is reduced.

The invention relates to the technical field of bioactive peptides, and discloses a walnut peptide with a neuroprotection function and a preparation method and application thereof, and predicts that a batch of small molecular walnut peptides have the neuroprotection function through enzymolysis of natural walnut protein, molecular weight screening and molecular docking. Experiments prove that the walnut peptide disclosed by the invention has a nerve protection function: the cell activity of nerve cells is improved, and the walnut peptide plays a role in preventing neurodegenerative diseases and other nerve-related diseases; the toxic effect of oxidative damage on nerve cells is relieved, nerve cell apoptosis is inhibited, the cell viability of the nerve cells is improved, and the function of treating nerve injury related diseases is achieved; the compound can be used for improving the activity of nerve cells, relieving oxidative damage of the nerve cells, adjusting the protein level of nerve damage related pathways, preparing related drugs for reducing blood pressure or resisting senile dementia or tumors and the like, and has a wide application prospect.

The invention relates to a plant composition as well as a preparation method and application thereof, and belongs to the technical field of health food. The plant composition is prepared from the following components in parts by mass: 5 to 30 parts of fructus gardeniae, 5 to 30 parts of fructus rubi and 5 to 30 parts of fructus lycii. The plant composition can effectively relieve liver oxidative stress and inflammation conditions, relieve and / or improve alcoholic liver injury, protect liver cells from oxidative injury, assist a body in resisting oxygen free radical invasion and maintain the health state of the body, does not affect the blood sugar level, and has the advantages of being reasonable in compatibility, good in quality, safe and effective.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention relates to the technical field of biological agents, in particular to abalone-derived polypeptide with antioxidant activity and a preparation method thereof. According to the invention, Haliotis discus hannai Ino is taken as a research object, a small peptide library is established by using a virtual enzymolysis technology from an important anti-oxidation pathway of cells, namely a Keap1-Nrf2 pathway, an abalone-derived anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1 is screened out by using a molecular docking technology, and then, the anti-oxidation activity of the abalone-derived anti-oxidation peptide DEDEDEDK is verified by using a cell oxidative damage model, so that the abalone-derived anti-oxidation peptide DEDEDEDK can be used for preparing the anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1. And a theoretical basis is provided for research and application of the antioxidant peptide.

The invention discloses an anti-aging composition containing PQQ and a preparation method of the anti-aging composition, and belongs to the field of medicines or health food, the anti-aging composition comprises PQQ, an antioxidant, collagen, hyaluronic acid and a plant extract, and the content of PQQ is 0.1-5%; 1%-10% of collagen; 0.05%-2% of hyaluronic acid; the antioxidant comprises the following components in percentage by weight: 0.5%-5% of vitamin C, 0.1%-3% of alpha-tocopherol and 0.05%-2% of beta-carotene; the composition contains PQQ and various antioxidants such as vitamin C, alpha-tocopherol, beta-carotene, coenzyme Q10 and glutathione, and the components have a synergistic effect, so that free radicals can be effectively removed, oxidative damage can be reduced, and cell aging can be delayed; most of the components in the composition are natural substances or are widely applied to the fields of food and health care products, the safety is high, and the quality and safety of the product can be ensured through the steps of strict preparation process control, accurate component dissolving method, pH value adjustment, filtration and the like.

A circadian rhythm-based time-sharing repair formula and use thereof. A day composition focuses on anti-oxidation, stress resistance and energy metabolism to resist oxidative damage and enhance day vitality and provide long-lasting antioxidation protection. A night composition focuses on DNA repair, anti-inflammation and nerve relaxation to promote cell regeneration and repair and continuously support night repair. The composition of the day composition and the night composition designed according to the circadian rhythm of human metabolism causes the corresponding activities to take effect at the best time, and components are designed to cooperate with each other according to different physiological needs during the day / night, which can greatly improve the anti-aging effect.

The invention discloses a plant lactobacillus with light aging resistance and an application thereof. The preservation number of the plant lactobacillus cj001 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35419. The fermentation supernatant of the plant lactobacillus cj001 shows good anti-aging activity and in-vitro antioxidant activity, and the growth ability of HaCaT cells is promoted. After the combined fermentation product of the plant lactobacillus cj001 and the lactobacillus paracasei Grx701 is subjected to ultraviolet radiation, the proliferation of HaCaT cells can be promoted, the endogenous antioxidant capacity of human skin cells can be enhanced, the photooxidation damage can be effectively relieved, and the combined fermentation product has a wide application value in the field of preparation of products with antioxidant, anti-aging, anti-ultraviolet damage, anti-photoaging and the like.

The invention belongs to the field of biological medicines, and discloses a stress-induced mesenchymal stem cell extract and a preparation method and application thereof.The preparation method comprises the steps that S1, mesenchymal stem cells are cultured under the high-temperature condition, swelling and cracking are conducted on the mesenchymal stem cells, or supernate obtained after culture of the mesenchymal stem cells is taken and filtered, and filtrate is obtained; the temperature of the high temperature ranges from 37.0 DEG C to 42.0 DEG C; s2, purifying the mesenchymal stem cell extract by adopting a molecular sieve exclusion chromatography method or a reverse chromatography method. Specific high-temperature stimulation is adopted to induce the mesenchymal stem cells, a purification method is combined, protein expression is promoted, the purity of the mesenchymal stem cell extract is improved, the prepared and obtained purified Alibitumen is used in a cell oxidative damage model, and the cell oxidative damage rate is increased. It is found that all the Aribitua derived from high-temperature stress culture has high oxidative damage repairing capacity.