Composition for dispelling effects of alcohol and protecting liver and application thereof

Abstract

The invention relates to the field of food and health care products, and discloses a composition for dispelling effects of alcohol and protecting liver, which comprises 20-100 parts of ampelopsis grossedentata leaf extract; 50 to 200 parts of a radix puerariae extract; 10-50 parts of a semen hoveniae extract; 10-50 parts of a turmeric extract; 10 to 100 parts of a yeast extract; 5-50 parts of a licorice extract; and 5 to 20 parts of pyrroloquinoline quinone disodium salt. The ampelopsis grossedentata leaf extract, the kudzuvine root extract and the hovenia dulcis thunb extract are utilized, alcohol decomposition is promoted, the blood ethanol concentration is reduced, and drunkenness symptoms are relieved. Puerarin improves activity of liver metabolic enzymes, accelerates discharge of ethanol and acetaldehyde, and reduces liver damage. The anti-oxidation effects of dihydromyricetin and PQQ can eliminate alcohol metabolism free radicals and protect liver cells. The components such as puerarin, hovenia dulcis thunb, curcumin and glycyrrhizic acid jointly promote liver cell regeneration, inflammation and oxidative damage are reduced, the levels of ALT and AST in serum are reduced, and liver health is maintained.

Description

Technical Field

[0001] The invention relates to the technical field of food and health products, and in particular to a composition for sobering up and protecting the liver and an application thereof. Background Art

[0002] With the continuous development of social economy and the change of lifestyle, drinking has become a common behavior in social activities, business entertainment and daily life. However, the health problems caused by drinking are also increasingly concerned. In particular, long-term excessive drinking is particularly harmful to the liver. The liver is the main organ for alcohol metabolism in the human body. After alcohol enters the human body, it will be broken down into acetaldehyde in the liver and further metabolized into acetic acid. However, the accumulation of alcohol and its metabolite acetaldehyde often causes serious damage to liver cells, and then causes a series of liver diseases such as alcoholic hepatitis, liver fibrosis, and alcoholic cirrhosis. Especially during the alcohol metabolism process, the large amount of free radicals produced will aggravate the oxidative stress response of liver cells, leading to cell function damage or even death.

[0003] In addition to drinking, modern people's accelerated pace of life, staying up late for a long time, irregular diet, high-pressure work and other factors also increase the burden on the liver, leading to liver dysfunction, further exacerbating liver cell damage and free radical generation. Therefore, the market demand for products with alcohol sobering and liver protection functions is increasing. In particular, products that can help the human body metabolize alcohol quickly, reduce the damage to liver cells caused by alcohol metabolites, and have antioxidant and liver health protection functions have become an urgent need for consumers.

[0004] There are some alcohol sobering and liver protection products on the market, but there are some shortcomings. The main function of many alcohol sobering products is to accelerate alcohol metabolism, but the scavenging effect on free radicals produced during metabolism is limited, and it cannot effectively prevent oxidative damage to liver cells. Moreover, most products have a single ingredient and a relatively limited alcohol sobering effect, which cannot meet the growing market demand. In addition, some products have problems such as slow alcohol sobering speed and unclear effect, and cannot provide comprehensive liver protection.

[0005] As people pay more and more attention to health, the concept of "medicine and food are of the same origin" has gradually become one of the main directions for the development of functional foods and health foods. Many natural plant extracts with the same origin as medicine and food, such as Agave ulmoides leaves, Pueraria root, Hovenia dulcis, turmeric, licorice, etc., have received widespread attention due to their natural safety and multiple health benefits. These plant extracts have been proven to have significant effects in hangover, liver protection, and anti-oxidation. For example, the dihydromyricetin (DMY) rich in Agave ulmoides leaves has a strong antioxidant capacity, can promote ethanol metabolism, and reduce alcohol damage to the liver; puerarin in Pueraria root helps to improve the liver's detoxification ability and improve the headache, dizziness and other discomfort symptoms caused by alcohol; Hovenia dulcis can accelerate the decomposition of ethanol, shorten the time of drunkenness, and reduce alcohol toxicity. In addition, ingredients such as curcumin and glycyrrhizic acid have also been widely studied and confirmed to have significant liver protection and antioxidant effects.

[0006] In addition, pyrroloquinoline quinone (PQQ), as a new type of natural bioactive substance, has received great attention in the fields of medicine and health care in recent years. PQQ has a strong ability to scavenge free radicals, and its antioxidant effect is significantly higher than that of vitamin C and vitamin E. PQQ can not only promote cell metabolism and improve mitochondrial function, but also inhibit the liver oxidative stress response caused by alcohol and reduce liver cell damage. Therefore, the addition of PQQ provides a more powerful antioxidant protection for the alcohol-detoxifying and liver-protecting composition.

[0007] Based on the above background, the present invention provides a scientifically formulated alcohol sobering and liver protecting composition to solve the deficiencies of the prior art. Summary of the invention

[0008] In view of the shortcomings of the alcohol-detoxifying and liver-protecting products in the prior art, such as poor alcohol-detoxifying effect, limited free radical scavenging ability, insufficient liver protection effect, etc., the present invention provides a composition for alcohol-detoxifying and liver-protecting and its application, which achieves rapid promotion of alcohol metabolism, reduction of damage to liver cells caused by alcohol and its metabolites, effective scavenging of free radicals, and enhancement of the liver's antioxidant capacity by scientifically combining a variety of plant extracts that are both medicinal and edible with pyrroloquinoline quinone (PQQ).

[0009] To achieve the above objectives, the present invention is implemented by the following technical scheme: a composition for sobering up and protecting the liver, the composition comprising the following components in parts by weight:

[0010] 20-100 parts of Ampelopsis grossedentata leaf extract;

[0011] 50-200 parts of Pueraria root extract;

[0012] 10-50 parts of Hovenia dulcis fruit extract;

[0013] 10-50 parts of turmeric extract;

[0014] 10-100 parts of yeast extract;

[0015] 5-50 parts of licorice extract;

[0016] 5 to 20 parts of pyrroloquinoline quinone disodium salt.

[0017] Preferably, the Ampelopsis grossedentata leaf extract is dihydromyricetin extracted from Ampelopsis grossedentata leaves, and the purity of the dihydromyricetin is 50% to 90%.

[0018] Preferably, the Pueraria root extract is puerarin extracted from Pueraria root, and the content of puerarin is 10% to 30%.

[0019] Preferably, the Hovenia dulcis fruit extract is a water-soluble active ingredient extracted from Hovenia dulcis fruit, and the content of the water-soluble active ingredient is 5% to 15%.

[0020] Preferably, a composition for sobering up and protecting the liver further comprises one or more pharmaceutically or food-acceptable excipients, wherein the excipients comprise one or more of a filler, a binder, a disintegrant, a lubricant or a coating agent, and the amount of the excipient added is 10% to 50% of the total weight of the composition.

[0021] Preferably, the auxiliary material is selected from maltitol, erythritol, maltodextrin, sorbitol, soluble starch or microcrystalline cellulose.

[0022] Preferably, the composition is in the form of tablets, granules, powders, oral liquids or beverages.

[0023] Preferably, a method for preparing a composition for sobering up and protecting the liver comprises the following steps:

[0024] (1) mixing an extract of Ampelopsis grossedentata leaves, a root of Pueraria lobata extract, a seed of Hovenia dulcis extract, a turmeric extract, a yeast extract, a liquorice extract and pyrroloquinoline quinone disodium salt according to the weight ratio;

[0025] (2) adding pharmaceutically or food-acceptable excipients to the mixture obtained in step (1), and granulating the mixture using a wet granulation process, adding 75% ethanol solution as a wetting agent;

[0026] (3) Drying the particles obtained in step (2) until the moisture content is less than 5%, and sterilizing them with ultraviolet light to obtain a final composition.

[0027] Preferably, the drying step is carried out at a temperature of 50° C. for a duration of 2 to 4 hours.

[0028] Preferably, the composition is used in the preparation of functional foods, health foods or medicines, and the application is to promote alcohol decomposition metabolism, reduce free radical generation and protect the liver.

[0029] The present invention provides a composition for sobering up and protecting the liver and its application, which has the following beneficial effects:

[0030] 1. The ingredients of the present invention such as the Ampelopsis grossedentata leaf extract, the Pueraria root extract and the Hovenia dulcis fruit extract work synergistically, which can effectively accelerate the decomposition and metabolism of alcohol in the body, reduce the ethanol concentration in the blood, and quickly relieve the discomfort symptoms after being drunk. Puerarin in the Pueraria root extract has been widely studied and confirmed to have the effect of enhancing the activity of liver alcohol metabolism enzymes, and can promote the rapid discharge of ethanol and acetaldehyde, thereby reducing the continuous damage of alcohol to the liver. This effect is of great help to people who drink frequently or occasionally drink too much, and helps to restore the sober state after drinking and shorten the duration of drunkenness.

[0031] 2. The present invention introduces dihydromyricetin (DMY) from the leaf extract of Agave sphenanthera and the potent antioxidant PQQ, which greatly improves the ability to scavenge free radicals. A large amount of reactive oxygen species (ROS) and free radicals are produced during alcohol metabolism, and these molecules can damage liver cells and aggravate the inflammatory response of the liver. The introduction of PQQ provides excellent antioxidant protection, and its ability to scavenge free radicals is significantly higher than that of traditional antioxidants such as vitamin C and vitamin E, which can effectively reduce the oxidative stress caused by alcohol, thereby protecting liver cells from free radical damage.

[0032] 3. The extracts of Pueraria root, Hovenia dulcis, Curcuma longa and Licorice in the present invention all have a clear liver protection effect. Puerarin in Pueraria root extract can not only promote liver cell regeneration and repair liver cells damaged by alcohol, but also improve headaches, nausea and other discomfort symptoms caused by drinking; Hovenia dulcis extract accelerates the catabolism of ethanol and reduces the toxicity of ethanol to the liver; Curcumin and glycyrrhizic acid effectively reduce the damage of alcohol and its metabolite acetaldehyde to liver cells by reducing the inflammatory response and oxidative damage of the liver. The synergistic effect of these ingredients can significantly reduce the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, maintain the normal functioning of liver function, and thus provide a strong liver protection effect.

[0033] 4. The present invention combines a variety of medicinal and edible plant extracts with PQQ in a scientific proportion, so that the functions of each component complement each other and produce a synergistic effect. Ingredients such as Ampelopsis grossedentata leaves, Pueraria root, and Hovenia dulcis can not only accelerate alcohol metabolism, but also protect the liver by scavenging free radicals, and the strong antioxidant capacity of PQQ further enhances the liver protection effect of the overall composition. Through this multi-component synergistic effect, the present invention can not only accelerate alcohol sobering, but also reduce the burden on the liver during alcohol metabolism, and comprehensively improve the effects of alcohol sobering and liver protection.

[0034] 5. The ingredients selected by the present invention, such as Agave ulmoides leaves, kudzu root, Hovenia dulcis, turmeric and licorice, are all natural plant extracts, which are safe ingredients of both medicinal and edible origin, and pyrroloquinoline quinone (PQQ) as a new type of food additive is also widely recognized as a safe and non-toxic substance. Therefore, the composition of the present invention has no toxic side effects and can be used for a long time. It is suitable for all kinds of people who need to sober up and protect the liver, including those who drink too much due to social activities or social activities, and consumers who hope to protect liver health through anti-oxidation. In addition, due to its natural safety, the product can also be widely used in the fields of functional foods, health foods and medicines.

[0035] 6. The composition of the present invention can be prepared into various dosage forms such as tablets, granules, powders, oral liquids or beverages according to different application scenarios to meet the needs of different consumers. These dosage forms are easy to carry and take, and are particularly suitable for social occasions, business activities or daily hangover needs. In addition, the convenient dosage form design also ensures that the product can take effect quickly in different environments, providing the effect of hangover and liver protection, which greatly facilitates the use of consumers. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 The present invention is a flow chart of the preparation method of the composition for sobering up and protecting the liver. DETAILED DESCRIPTION

[0037] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Please see attached Figure 1 :

[0039] Example 1: Preparation of tablets

[0040] (1) Preparation of raw materials and auxiliary materials: Weigh 100 parts of Agave sutchuenensis leaf extract (rich in dihydromyricetin, purity 50% to 90%), 50 parts of Pueraria root extract (puerarin content 10% to 30%), 30 parts of Hovenia dulcis extract (water-soluble active ingredient content 5% to 15%), 10 parts of Curcuma longa extract, 20 parts of yeast extract, 10 parts of Licorice extract, and 10 parts of Pyrroloquinoline Quinone Disodium Salt (PQQ). Mix the above raw materials to a total mass of 23 g. Weigh 57 g of maltitol as an auxiliary material and mix it thoroughly with the above raw materials to ensure that each component is evenly distributed.

[0041] (2) Wet granulation: Add an appropriate amount of 75% ethanol solution to the mixed raw materials by spraying to wet the materials and form granules. The ethanol solution is used as a wetting agent for granulation to ensure that the components are fully and evenly mixed to avoid component separation.

[0042] (3) Drying: The wet granulated particles were placed in a 50°C constant temperature oven for 4 hours to ensure that the moisture content of the particles was less than 5% to prevent the particles from becoming unstable in subsequent processes due to excessive moisture. After drying, ultraviolet sterilization was used for 30 minutes to further ensure the sterile environment of the particles and reduce the risk of contamination.

[0043] (4) Tablet compression: The dried granules are tablet compressed to obtain 100 tablets, each weighing 0.8 g. The tablet dosage form is easy to carry and take, and ensures the stability and absorption effect of the active ingredient.

[0044] Summary: The tablets showed strong activity in the hangover-relief effect due to the compatibility of Agave sphenanthera leaf and Pueraria root extract, especially the alcohol metabolism rate was significantly improved. In addition, due to the presence of yeast extract, the tablets also had certain effects on liver protection and antioxidant capacity.

[0045] Example 2: Preparation of granules

[0046] (1) Mixing raw materials and auxiliary materials: Weigh 80 parts of Ampelopsis grossedentata leaf extract, 100 parts of Pueraria root extract, 20 parts of Hovenia dulcis extract, 10 parts of Curcuma longa extract, 30 parts of yeast extract, 10 parts of Licorice extract and 20 parts of Pyrroloquinoline quinone disodium salt (PQQ), with a total weight of 27 g. Weigh 173 g of maltodextrin as an auxiliary material, and fully mix the above raw materials and auxiliary materials to ensure uniform particles and improve dissolution rate and taste.

[0047] (2) One-step granulation: Add the mixed materials into a boiling granulator and spray 75% ethanol solution as a wetting agent for granulation. This process ensures uniform granulation and moderate particle size, which is convenient for administration and dissolution.

[0048] (3) Sterilization: After granulation, the granules are sterilized with ultraviolet light for 30 minutes to ensure that the granules are not contaminated by microorganisms during packaging and storage.

[0049] (4) Packaging: The sterilized granules are packaged to obtain 100 bags of granules, each bag containing 2 g. The granule dosage form is easy to dissolve in water and is suitable for oral administration.

[0050] Summary: The granules have a significant effect on sobering up due to the high content of Pueraria extract and PQQ, especially in terms of rapid sobering up and liver protection. The granules have good solubility and are suitable for people who need to take them quickly. Its antioxidant properties are better than tablets, showing a strong free radical scavenging ability.

[0051] Example 3: Preparation of tablets

[0052] (1) Mixing raw materials and auxiliary materials: Weigh 80 parts of Ampelopsis serrulata leaf extract (rich in dihydromyricetin, purity 50% to 90%), 100 parts of Pueraria root extract (puerarin content 10% to 30%), 20 parts of Hovenia dulcis extract, 10 parts of Curcuma longa extract, 30 parts of yeast extract, 10 parts of Licorice extract, and 20 parts of Pyrroloquinoline Quinone Disodium Salt (PQQ). The total mass of the composition is 27 g. Weigh 53 g of maltitol as an auxiliary material and mix thoroughly to ensure that all ingredients are evenly distributed.

[0053] (2) Wet granulation: Use a sprayer to add 75% ethanol solution to the mixed materials as a wetting agent for wet granulation. Wet granulation helps ensure the density and stability of the tablets.

[0054] (3) Drying: The wet granulated material is placed in a 50°C oven for 4 hours to ensure that the moisture content of the material is reduced to less than 5%. After drying, ultraviolet sterilization is performed for 30 minutes to ensure the hygiene and safety of the tablets.

[0055] (4) Tablet compression: The dried granules are compressed into tablets, each weighing 0.8 g. The tablet design ensures that it is easy to take and carry while maintaining the stability of the active ingredients.

[0056] Summary: This tablet has the strongest antioxidant and free radical scavenging capabilities due to its high content of PQQ and kudzu root extract. In the free radical scavenging experiment, the effect of this formula is the most significant. In addition, the liver protection and alcohol sobering effects are also very prominent, suitable for people who drink alcohol for a long time or need continuous liver protection.

[0057] Example 4: Preparation of powder

[0058] (1) Mixing raw materials and auxiliary materials: Weigh 50 parts of Ampelopsis grossedentata leaf extract, 200 parts of Pueraria root extract, 30 parts of Hovenia dulcis extract, 10 parts of Curcuma longa extract, 20 parts of yeast extract, 10 parts of Licorice extract and 10 parts of Pyrroloquinoline quinone disodium salt (PQQ), with a total weight of 33 g. Weigh 268 g of maltodextrin as an auxiliary material, and fully mix it with the above raw materials to ensure that the powder is fine and uniform.

[0059] (2) Screening: The mixed materials are screened through a 60-mesh standard sieve to ensure fine and uniform particles and improve the solubility and taste of the powder.

[0060] (3) Sterilization: After screening, use ultraviolet sterilization for 30 minutes to ensure the sterility of the powder and avoid the growth of microorganisms during storage.

[0061] (4) Packaging: The sterilized powder is packaged to obtain 100 bags of powder, each bag containing 3 g of powder. The powder form is suitable for dissolving in liquid for quick consumption and is easy to carry and use.

[0062] Summary: Due to the high content of Pueraria extract and the high absorbability of the powder, the powder can take effect quickly and is especially suitable for people who are in urgent need of hangover relief. Due to the low content of yeast extract and PQQ, the antioxidant effect is slightly insufficient compared with other embodiments, but it performs well in terms of rapid hangover relief and liver protection.

[0063] Example 5: Preparation of tablets

[0064] (1) Mixing raw materials and auxiliary materials: Weigh 50 parts of Ampelopsis grossedentata leaf extract (rich in dihydromyricetin, purity 50% to 90%), 200 parts of Pueraria root extract, 30 parts of Hovenia dulcis extract, 10 parts of Curcuma longa extract, 20 parts of yeast extract, 10 parts of Licorice extract and 10 parts of Pyrroloquinoline quinone disodium salt (PQQ), with a total weight of 33 g. Weigh 47 g of maltitol as an auxiliary material, and mix thoroughly to ensure uniformity of the ingredients.

[0065] (2) Wet granulation: The uniformly mixed materials are added to 75% ethanol solution using a sprayer for wet granulation. This process can form granules with stable structure and good tableting effect.

[0066] (3) Drying and sterilization: The wet granulated material is placed in a 50°C oven for 4 hours to dry the granule moisture content to below 5%. After drying, ultraviolet sterilization is performed for 30 minutes to ensure the safety of the preparation.

[0067] (4) Tablet pressing: The dried and sterilized granules are pressed into tablets by a tablet press. Each tablet weighs 0.8 g, ensuring convenient administration and suitable for daily use.

[0068] Summary: This tablet performs well in the effect of sobering up, especially the higher proportion of Pueraria extract ensures the acceleration of alcohol metabolism. However, due to the relatively low content of PQQ and yeast extract, it is slightly insufficient in terms of antioxidant effect. Overall, this tablet is suitable for people who drink a lot on a daily basis and can provide a strong liver protection effect.

[0069] Embodiment 6:

[0070] In order to test the efficacy of different compositions, the compositions of Example 2 and Example 4 were processed into tablets of the same weight as Example 1 by changing the amount of excipients while keeping the content of the effective ingredients unchanged, thus forming Example 3 and Example 5. Therefore, the performance tests of the tablet compositions prepared in Example 1, Example 3 and Example 5 were carried out as follows:

[0071] 1. Free radical scavenging ability test

[0072] The tablet samples of Example 1, Example 3 and Example 5 were respectively prepared into 2 mg / mL solutions, and ascorbic acid was prepared into 2 mg / mL solution as a positive control for measurement.

[0073] (1) Determination of DPPH free radical scavenging ability

[0074] Use 95% ethanol to prepare 0.5mmo l / L DPPH solution. Mix 100μL 0.5mmo l / L DPPH solution and 100μL sample solution, and incubate at 37℃ in the dark for 30min. Measure the absorbance at 517nm, recorded as A1; ultrapure water is used as a blank control, and the absorbance is recorded as A0; ultrapure water is used instead of DPPH solution and mixed with 100μL sample solution, and incubate at 37℃ in the dark for 30min, and the absorbance is recorded as A2. Calculate according to the following formula: DPPH clearance rate / % = (1-(A1-A2) / A0)×100%

[0075] (2) Determination of ABTS cation radical scavenging ability

[0076] Prepare 0.1mmol / L ABTS cation working solution with a stable absorbance (734nm) of 0.7-0.8. Mix 100μL of sample solution with 100μL ABTS cation working solution, and measure the absorbance at 734nm wavelength, which is recorded as A1. Replace the sample with 100μL ultrapure water and measure the absorbance, which is recorded as A0; replace the ABTS cation working solution with ultrapure water and mix with 100μL sample solution, incubate at 37℃ in the dark for 30min, measure the absorbance, and record it as A2. Calculate according to the following formula: ABTS clearance rate / % = (1-(A1-A2) / A0)×100%

[0077] The free radical scavenging ability test results are shown in Table 1. Compared with ascorbic acid, the compositions in the examples have better scavenging abilities against DPPH, ABTS, etc., among which Example 3 has the highest scavenging rate.

[0078] Table 1 Free radical scavenging ability of different compositions

[0079] Group DPPH clearance rate (%) ABTS cation clearance rate (%) ascorbic acid 54.6±4.8 46.3±5.9 Example 1 76.9±3.2 64.5±3.4 Example 3 85.2±4.5 72.4±3.7 Example 5 76.8±2.5 66.7±2.8

[0080] It can be seen from the above table that the composition of the present invention has a good free radical scavenging ability.

[0081] 2. Functional test to protect liver cells

[0082] Human ChangLiver cells in the logarithmic growth phase were collected and cultured in DMEM medium containing 10% fetal bovine serum. The cell density was adjusted to 1×10 5 / mL. 100 μL was inoculated into a 96-well plate and randomly divided into 5 groups, namely the control group, model group, embodiment 1 intervention group, embodiment 3 intervention group and embodiment 5 intervention group, with 3 replicates in each group. 100 μL of the corresponding sample solution was added to the intervention group, and the blank group and the model group were added with an equal amount of normal saline. After culturing in a 5% CO2, 37°C incubator for 24 hours, the model group and the intervention group were added with a final volume fraction of 5% alcohol, and the blank group continued to add an equal amount of normal saline and continued to culture for 12 hours. An equal amount of cell-free culture medium + sample solution was used as the blank control group. After culture, 10 μL of CCK-8 solution was added to each well, incubated in a 37°C incubator for 4 hours, and the absorbance (OD) value at a wavelength of 450nm was measured. The cell proliferation rate was calculated according to the following formula: Cell proliferation rate = (OD experimental group-OD blank control group) / (OD control group-OD blank control group) × 100%.

[0083] The experimental results showed that the liver cell proliferation rate in the model group was 42.5±5.2%, which was significantly lower than that in the control group (100%). Compared with the model group, the liver cell proliferation rate in each composition intervention group was significantly increased, which were 75.3±7.6%, 88.2±5.7%, and 79.0±8.5%, respectively, indicating that the composition has a strong protective effect on alcohol-damaged liver cells, among which the liver cell proliferation rate of Example 3 was the highest and the protective effect was the best.

[0084] 3. Determination of indicators related to the alcohol metabolism capacity of hepatocytes

[0085] Human ChangL iver cells in logarithmic growth phase were collected and cultured in DMEM medium containing 10% fetal bovine serum. The cell density was adjusted to 1×10 e 6 / mL. Take 1mL of cells and inoculate them in a 6-well plate. The rest of the experimental scheme is the same as above. After the cells are added with alcohol and cultured for 12 hours, the supernatant is collected and the ALT and AST activities are determined according to the instructions of the kit (product of Nanjing Jiancheng Bioengineering Institute). The cells are digested with trypsin, centrifuged and collected, washed twice with PBS, and the cell suspension of each group is ultrasonically broken until transparent, and the supernatant is centrifuged and the SOD, GSH, and MDA contents are determined according to the instructions of the kit (product of Nanjing Jiancheng Bioengineering Institute).

[0086] The experimental results show (Table 2) that after 12 hours of alcohol damage to liver cells, the ALT and AST activities in the culture fluid of the model group were higher than those in the control group; compared with the model group, the ALT and AST activities in the culture fluid of the composition intervention group were lower, indicating that each composition can effectively improve alcohol metabolism and reduce cell damage, among which the composition of Example 3 has the best effect. After 12 hours of alcohol damage to liver cells, the MDA level of cells in the model group was higher than that in the control group, while the GSH and SOD levels were lower; compared with the model group, the MDA content of the intervention group was lower, and the GSH content and SOD activity were both higher. The results show that the stress response of cells intervened by the composition to alcohol damage was reduced, and the antioxidant activity was increased, among which the composition of Example 3 had the best effect.

[0087] Table 2 Effects of combination intervention on the release of ALT and AST in extracellular fluid of liver cells and the levels of MDA, GSH and SOD in liver cells

[0088]

[0089]

[0090] Comparison and summary of experimental results:

[0091] Through the performance tests of different compositions, combined with the DPPH free radical scavenging experiment, the hepatocyte proliferation experiment and the test of the alcohol metabolism capacity of hepatocytes, the following results can be summarized:

[0092] Free radical scavenging ability: Example 3 performed best, mainly due to the synergistic effect of its higher content of PQQ and yeast extract. Example 1 and Example 5 performed second, and Example 4 had a slightly lower free radical scavenging ability due to its lower PQQ content.

[0093] Liver cell protection ability: Examples 3 and 5 had the highest liver cell proliferation rates, showing excellent liver protection effects in the alcohol injury model. Examples 1 and 4 performed relatively moderately.

[0094] Effect of sobering up: Since Examples 2 and 4 contain a higher proportion of Pueraria root extract, the sobering up effect is the most significant and they are suitable for people who are in urgent need of sobering up.

[0095] In summary, each embodiment shows its own advantages in sobering up, protecting the liver and anti-oxidation according to different ratios and dosage forms, and the most suitable formula and dosage form can be selected according to specific needs.

[0096] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composition for sobering up and protecting the liver, characterized in that: The composition comprises the following components in parts by weight: 20-100 parts of Ampelopsis grossedentata leaf extract; 50-200 parts of Pueraria root extract; 10-50 parts of Hovenia dulcis fruit extract; 10-50 parts of turmeric extract; 10-100 parts of yeast extract; 5-50 parts of licorice extract; 5 to 20 parts of pyrroloquinoline quinone disodium salt.

2. The composition for sobering up and protecting the liver according to claim 1, characterized in that: The Ampelopsis grossedentata leaf extract is dihydromyricetin extracted from the leaves of Ampelopsis grossedentata, and the purity of the dihydromyricetin is 50% to 90%.

3. The composition for sobering up and protecting the liver according to claim 1, characterized in that: The pueraria root extract is puerarin extracted from pueraria root, and the content of puerarin is 10% to 30%.

4. The composition for sobering up and protecting the liver according to claim 1, characterized in that: The Hovenia dulcis fruit extract is a water-soluble active ingredient extracted from Hovenia dulcis fruit, and the content of the water-soluble active ingredient is 5% to 15%.

5. The composition for sobering up and protecting the liver according to claim 1, characterized in that: It also includes one or more pharmaceutically or food-acceptable excipients, including one or more of fillers, binders, disintegrants, lubricants or coating agents, and the amount of the excipients added is 10% to 50% of the total weight of the composition.

6. The composition for sobering up and protecting the liver according to claim 5, characterized in that: The auxiliary material is selected from maltitol, erythritol, maltodextrin, sorbitol, soluble starch or microcrystalline cellulose.

7. The composition for sobering up and protecting the liver according to claim 1, characterized in that: The dosage form of the composition is tablet, granule, powder, oral liquid or beverage.

8. A method for preparing a composition for sobering up and protecting the liver according to any one of claims 1 to 7, characterized in that: The following steps are involved: (1) mixing an extract of Ampelopsis grossedentata leaves, a root of Pueraria lobata extract, a seed of Hovenia dulcis extract, a turmeric extract, a yeast extract, a liquorice extract and pyrroloquinoline quinone disodium salt according to the weight ratio; (2) adding pharmaceutically or food-acceptable excipients to the mixture obtained in step (1), and granulating the mixture using a wet granulation process, adding 75% ethanol solution as a wetting agent; (3) Drying the particles obtained in step (2) until the moisture content is less than 5%, and sterilizing them with ultraviolet light to obtain a final composition.

9. The method for preparing a composition for sobering up and protecting the liver according to claim 8, characterized in that: The drying step is carried out at a temperature of 50°C and lasts for 2 to 4 hours.

10. Use of the composition according to any one of claims 1 to 7 in the preparation of functional foods, health foods or medicines, characterized in that: The application is to promote alcohol decomposition metabolism, reduce free radical generation and protect the liver.

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