202 results about "Skin photoaging" patented technology

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention discloses aloe exosome-like nano-vesicles capable of delaying skin photoaging and an extraction method and application thereof, and belongs to the technical field of exosome-like nano-vesicle extraction. The extraction method of the aloe exosome-like nano-vesicles is designed and optimized, the yield of the aloe exosome-like nano-vesicles is obviously improved, and the extracted aloe exosome-like nano-vesicles can obviously delay skin photoaging and have an obvious improvement effect on skin fibroblast aging damage caused by ultraviolet radiation; a potential treatment means is provided for preventing or treating actinic keratosis caused by ultraviolet radiation and further possible squamous cell carcinoma.

The invention relates to an application of metformin in preparation of skin anti-photoaging drugs or medical instruments. Through verification, the metformin has the effects of promoting the formation of autophagosome, recovering the patency of autophagy flow and remodeling an autophagy-ECM metabolism regulation network. After the composition is applied to the skin (such as a corium layer), the effect of resisting skin photoaging is achieved by adjusting an AMPK signal channel. The metformin is used as an active ingredient to prepare a medicine or a medical instrument, and is beneficial to clinically anti-photoaging application of the skin.

The invention provides a hydroxytyrosol-loaded soluble microneedle as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The microneedle comprises a substrate layer and a needle body arranged on the surface of the substrate layer, the needle body comprises 0.01%-20% of hydroxytyrosol and 1%-10% of hyaluronic acid, the molecular weight of the hyaluronic acid is 100-1000 kDa, and the% is mass percentage content w / w or mass volume percentage content w / v. The soluble microneedle is mild and controllable in preparation process, does not need high temperature, is simple in preparation and use method, and is green, safe and pollution-free; the matrix material is simple, no organic solvent is used, only water is used as a solvent and an adhesive, and no extra cross-linking agent needs to be added; the hydrogel is good in needle forming property, good in biocompatibility, high in safety, high in mechanical strength, excellent in transdermal drug delivery effect and capable of remarkably improving skin photoaging.

The invention discloses application of a glucosyl modified quercetin derivative and a composition containing the glucosyl modified quercetin derivative. Specifically, the invention discloses an application of glucosyl modified quercetin as an anti-skin photoaging agent. According to the application disclosed by the invention, glucosyl modified quercetin, especially glucosyl modified isoquercitrin, has a remarkable effect in the aspect of resisting skin photoaging; cosmetics prepared from glucosyl modified quercetin have good anti-aging ability, and can significantly improve percutaneous water loss, skin elasticity and skin roughness.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention discloses a modeling device for a mouse skin light aging experiment, and relates to the technical field of skin light aging experiments, the modeling device comprises a box body, a box cover is hinged to the upper end of the box body, a bottom plate is fixedly arranged in the box body, a plurality of fixed partition plates are arranged at the upper end of the bottom plate, and adjusting partition plates are arranged on one sides of the fixed partition plates; fixed sliding plates are arranged on one sides of the fixed partition plates in a sliding mode, telescopic sliding plates are arranged at one ends of the fixed sliding plates in a sleeving and sliding mode, a linkage mechanism is arranged on one side of the box body, and irradiation components are arranged in the box cover and above the experiment channel. The linkage mechanism drives the adjusting partition plate, the fixed sliding plate and the telescopic sliding plate to move, so that when an irradiation experiment is carried out, the space of an experiment channel can be reduced, the activity range of a mouse is limited, and the distance between the two ultraviolet lamp sources and the irradiation angle of the ultraviolet lamp sources can be adjusted through the adjusting mechanism; therefore, irradiation in different states can be simulated conveniently.

The invention belongs to the technical field of biology, and particularly relates to application of ginsenoside Rh1 in preparation of a product for relieving skin photoaging. It is found for the first time that the rare ginsenoside Rh1 efficiently removes ROS caused by UV and inhibits cell aging by activating a Sirt3 / Nrf2 / HO-1 pathway, and a brand new strategy is provided for developing a natural anti-photoaging preparation with repair and protection functions.

The invention discloses application of tea extracellular vesicles in preparation of a product for preventing and / or treating UVB-induced skin photoaging, and belongs to the technical field of application of the tea extracellular vesicles. The tea extracellular vesicles are derived from tea leaves and / or stem leaves and are prepared by a method of combining enzymolysis, multi-stage filtration and ultracentrifugation, and the particle size of the tea extracellular vesicles is mainly distributed in a range of 60-100nm. Experiments show that the tea extracellular vesicles can effectively reduce active oxygen accumulation and lipid peroxidation caused by UVB radiation, repair mitochondrial membrane potential and inhibit expression of matrix metalloproteinase and inflammatory factors, so that photoaging symptoms such as skin wrinkles, erythema and barrier function damage are relieved. The tea extracellular vesicle provided by the invention has the advantages of natural raw materials, good biocompatibility, small side effect and the like, can be used for preparing a plurality of external dosage forms such as gel, cream, spray and the like, and provides a safe and effective new natural product choice for prevention and treatment of skin photoaging.

The invention belongs to the technical field of biological materials, and particularly relates to a multifunctional beauty microneedle for improving skin photoaging and a preparation method and application thereof. According to the invention, water-soluble hyaluronic acid and chitosan / beta-sodium glycerophosphate temperature-sensitive hydrogel are used as a polymer of a microneedle substrate layer, and the polymer comprises exosome of human adipose-derived mesenchymal stem cells and epigallocatechin gallate. When the microneedle is inserted into the skin, hyaluronic acid is quickly dissolved after contacting with tissue fluid, the needle tip is implanted into the skin, and the CS / beta-GP temperature-sensitive hydrogel is in a solid-gel-like state and can slowly release two cosmetic active ingredients in a long-acting manner under the influence of environment temperature and humidity; the facial mask has the effects of promoting tissue regeneration, reducing hyperpigmentation, replenishing water, preserving moisture, resisting oxidation, delaying skin aging and the like, so as to achieve the medical beauty effects of reducing wrinkles, whitening, preserving moisture, rejuvenating the face and the like. The microneedle provided by the invention can be further prepared into a patch, and is beneficial to treatment of wrinkles and stains at specific parts.

The invention discloses an anti-aging composition for preventing light aging and application thereof, the anti-aging composition comprises a Rosa rugosa extract, a Caesalpinia spinosa fruit extract, a sunflower bud extract and a Pseudoalteromonas fermentation product extract, and the anti-aging composition can be added into various skin care products to reduce cell damage caused by light radiation such as ultraviolet light, infrared light and blue light; in sunscreen cosmetics, the sunscreen effect can be improved, the use amount of chemical sunscreen agents and physical sunscreen agents is reduced, and the safety and mildness of the sunscreen cosmetics are improved; damage caused by optical radiation is effectively prevented, meanwhile, skin elasticity is improved, skin wrinkles are reduced, and the effect of resisting skin photoaging is achieved. The effectiveness of the composition is proved through a large number of experiments, each component is irreplaceable, the addition range of the composition is also an optimal range, and the effect and cost of the composition are both the optimal range.

The invention provides a whitening and anti-aging extracellular vesicle compound as well as a preparation method and application thereof. The extracellular vesicle compound comprises extracellular vesicles derived from mesenchymal stem cells and active ingredients loaded in the extracellular vesicles, the active component comprises any one or a combination of at least two of retinoic acid, arbutin, panthenol, nicotinamide, ectoine, vitamine, hyaluronic acid, vitamin C, resveratrol or retinol; the ratio of the extracellular vesicles to the active ingredients is (1-10) * 10 < 8 >: (0.01-1) mg. The skin-care active ingredients are entrapped in the extracellular vesicles derived from the mesenchymal stem cells, so that the delivery efficiency of the skin-care active ingredients can be remarkably improved, the direct irritation of free components to the skin can be reduced, the generation of matrix metalloproteinase-1 (MMP-1) and melanin can be effectively inhibited, the light aging process of the skin can be delayed, and the skin-care composition has excellent whitening and anti-aging effects.

The invention discloses an application of bulbus lilii glycoside B. The application is that bulbus lilii glycoside B is used as a unique active component or one of the active components to prepare a product for preventing or / and treating skin photoaging diseases. Experimental results of the invention show that lilium brownii glycoside B can significantly improve the cell viability of UVA-induced human skin fibroblasts, can reduce the number of beta-galactosidase positive cells in the UVA-induced human skin fibroblasts and reduce lipofuscin deposition, and can significantly improve the photoaging of the UVA-induced skin fibroblasts. Meanwhile, the skin photoaging grading of the back of a C57BL / 6 mouse induced by UVA can be remarkably improved. Therefore, the bulbus lilii glycoside B is expected to be used as an active ingredient for preparing medicines or cosmetics for preventing or / and treating the skin photoaging diseases, and particularly has important significance and remarkable application value for preventing or / and treating the UVA-induced skin photoaging diseases.

The invention aims to solve the problems that the existing skin photoaging evaluation technology can only realize static detection, lacks environment dynamic monitoring and cannot capture day and night changes of physiological parameters. According to the system, key parameters such as skin percutaneous moisture loss, tissue impedance and temperature are continuously measured through a wearable flexible electronic patch, a portable environment monitoring module is used for measuring UVA / UVB intensity and pollutants and is linked with satellite meteorological data to construct a personal exposure map, and a non-invasive microneedle sensing technology is used for detecting skin interstitial fluid biomarkers. A time-space diagram neural network model is used for fusing data, oxidative stress, collagen degradation and environmental toxicity are comprehensively considered, a light aging index is output, clinical intervention suggestions are provided through a data analysis and prediction module, and dynamic accurate evaluation is achieved.

The invention relates to an application of an acellular extracellular matrix as an active ingredient in preparation of a medicine or a medical instrument for treating skin aging. The acellular extracellular matrix comprises collagen and glycosaminoglycan, and the DNA content is lower than 50 ng / mg dry weight. Through verification, the technical scheme provided by the invention not only can recover the dermis volume, but also can reverse degenerative vascular changes. And the polypeptide has low immunogenicity, retains a plurality of bioactive components, has regeneration potential in a plurality of tissues, and can be effectively applied to the field of skin repair and regeneration when being used as an active component to prepare medicines or medical instruments.

The invention discloses an application of eclipta source extracellular vesicles. The extracellular vesicles are extracted from the eclipta by adopting an overspeed combined ultrafiltration centrifugation method, and the extracted vesicles are complete in morphology and good in dispersity, and have the characteristics of high yield, high separation speed, low cost and easiness in large-scale production. The extracted vesicles can be successfully taken by animal cells. The yerbadetajo herb alcohol extract has the advantages that the yerbadetajo herb alcohol extract has antioxidant and anti-aging effects after being taken in, ultraviolet-induced matrix metalloproteinase 1 (MMP1) expression can be obviously reduced, collagen I (COL-I) generation can be promoted, ultraviolet-induced skin light aging can be improved, and the effect is better than that of the yerbadetajo herb alcohol extract, so that the yerbadetajo herb alcohol extract can be used for preparing the skin The eclipta-derived extracellular vesicles provided by the invention provide a new strategy for skin aging and anti-aging treatment.

The invention belongs to the technical field of biological medicine and skin repair, and particularly relates to a recombinant extracellular matrix protein composition and application thereof in preparation of products for resisting skin photoaging. The recombinant extracellular matrix protein composition is obtained by mixing recombinant humanized III collagen, recombinant humanized chemical fibronectin and recombinant humanized elastin. Tests show that the recombinant extracellular matrix protein composition can improve the survival rate of cells damaged by UVB, reduce the generation of ROS, improve the activity of SOD, CAT and GSH-Px and the level of GSH, and reduce the content of lipid peroxide MDA. The recombinant extracellular matrix protein composition has an effective anti-oxidative damage effect and can resist skin cell damage caused by UVB.

The present invention relates to a method for preparing controlled-release enzyme-loaded microspheres for preventing and treating skin photoaging and their application, belonging to the field of pharmaceutical engineering drug preparations. The present invention's preparation method produces controlled-release enzyme-loaded microspheres loaded with cathepsin D and cathepsin K, combining the advantages of microsphere controlled release with the therapeutic strategy of cathepsins. The resulting microspheres have the effect of preventing and treating skin photoaging, representing a major breakthrough in the research and development of specific drugs for preventing and treating photoaging and related skin lesions. The present invention can provide a new strategy for developing better small-molecule drugs for the prevention and treatment of photoaging and related skin diseases, and has important theoretical and practical value.

The invention relates to a preparation method of a small nucleic acid combined microneedle and application of the small nucleic acid combined microneedle to skin light injury repair, and particularly discloses a microneedle composition which comprises a compound of SNAP25 specific siRNA and nano-selenium and hyaluronic acid, the nucleotide sequence of a positive-sense strand of the siRNA is shown as SEQ ID NO.1, and the nucleotide sequence of an antisense strand of the siRNA is shown as SEQ ID NO.2. The invention further discloses a preparation method of the microneedle composition. The microneedle composition provided by the invention can realize stable delivery of siRNA, inhibit UV-induced skin photoaging related gene expression, increase repair of skin extracellular matrix and significantly improve skin light damage, and is suitable for clinical transformation application.

This invention provides a novel application of IdeS, which is used to prepare products addressing photoaging of the skin. These products include skincare products and pharmaceuticals for the prevention and / or treatment of photoaging. The pharmaceuticals are in the form of ointments, and the skincare products are used as adjunct treatments for preventing and treating photoaging. IdeS treatment significantly improves the extensive erythema, wrinkles, desquamation, inflammation, and thickened stratum corneum observed in photoaging model mice. It has been developed as a novel biological therapeutic drug with strong pharmacological effects, few side effects, and avoidance of allergic reactions, thus providing a basis for the clinical treatment of photoaging and photodamage.

The invention discloses an application of R-3-hydroxybutyric acid (R-3-hydroxybutyric acid, 3HB) or a derivative of the R-3-hydroxybutyric acid in delaying and preventing light aging of skin. Experiments prove that the 3HB or the derivative thereof can remarkably improve the skin appearance of a mouse with photoaged skin, improve the antioxidant capacity of skin tissue, remarkably recover the thickness of the epidermal layer and the corium layer of the skin, maintain the collagen content in the skin, reduce the denaturation degree of elastic fibers in the skin and improve the skin aging resistance of the mouse. The obvious effect of delaying and preventing the skin photoaging is achieved. The invention provides a novel skin aging treatment medicine or cosmetic candidate compound which has a wide clinical application prospect and good social and economic benefits.

The invention provides a ferulate compound as well as a preparation method and application thereof, the ferulate compound is (5-formyl-2-furan) methyl dihydroferulate, the ferulate compound is prepared by the following steps: extracting a dry whole herb water extract of eyebright by using ethyl acetate, recovering a solvent to obtain an ethyl acetate extraction part, and separating by using normal-phase silica gel column chromatography to obtain the (5-formyl-2-furan) methyl dihydroferulate. Petroleum ether-ethyl acetate is used as an eluent for gradient elution, a fraction Fr.2 is obtained and separated by normal-phase silica gel column chromatography to obtain a fraction Fr.2. 2, Sephadex LH-20 gel column chromatography is performed to obtain a fraction Fr.2. 2.1, MC I column chromatography is performed to separate the fraction Fr.2. 2.1, methanol-water is used as an eluent for gradient elution to obtain a fraction Fr.2. 1.1, and then preparative HPLC (High Performance Liquid Chromatography) is performed to obtain the HaCaT cell activity. The composition has a protection effect on HaCaT cell oxidative damage caused by UVB, and can effectively resist skin photoaging.

The invention discloses an application of an engineered mRNA (messenger Ribonucleic Acid) medicine for coding COL3A1 protein in resisting skin photoaging. According to the method, accurate rational design and optimization are carried out on the 5'untranslated region (UTR) of the hCOL3A1 mRNA, so that the translation efficiency and the protein yield of the hCOL3A1 mRNA are greatly improved, the expression level of the hCOL3A1 target protein far beyond the conventional sequence in vivo and in vitro is realized, and the type III collagen is efficiently and endogenously synthesized in skin cells. Experimental results show that the engineered mRNA can effectively reduce the skin oxidative stress level, inhibit cell aging and promote endogenous synthesis and deposition of corium layer collagen, so that the barrier function of photoaged skin is remarkably improved, the dermis thickness is increased, the tissue structure is repaired, and systemic toxicity or immune side effects are not observed. The invention provides a novel strategy with high efficiency and safety for resisting skin photoaging.

The invention provides royal jelly polypeptide CMKI-21 capable of promoting wound healing and resisting skin photoaging and application of the royal jelly polypeptide CMKI-21, and belongs to the technical field of biological medicine. The amino acid sequence of the royal jelly polypeptide is KKELVREVGFEICFKDHHEKI, and the royal jelly polypeptide is obtained by a solid-phase chemical synthesis method. The royal jelly polypeptide CMKI-21 disclosed by the invention is small in molecular weight, simple and feasible in preparation method, high in purity and good in biocompatibility, and can remarkably promote proliferation and migration of HaCaT cells, accelerate the healing rate of full-thickness skin defect wounds of mice, relieve light damage of the HaCaT cells and relieve skin light aging and sunburn caused by ultraviolet (UVB). The royal jelly polypeptide has the potential to be applied to various clinical skin wounds as a wound healing agent, and can also be applied to medicines and cosmetics for resisting skin photoaging and repairing sunburn.

The invention relates to the technical field of skin care, and discloses a composition for improving skin aging and a preparation method and application thereof. The feed additive is prepared from the following raw materials in parts by mass: 18 to 30 parts of yeast extract, 18 to 30 parts of rhizoma curcumae longae extract, 12 to 25 parts of mixed herbal extract, 12 to 25 parts of linear aspartame extract, 5 to 15 parts of green tea extract, 0 to 4 parts of vitamin B3, 0 to 3 parts of vitamin B5, 0 to 2 parts of vitamin B7, 0 to 2 parts of vitamin B12, 0 to 1 part of vitamin B6, 0 to 1 part of vitamin B1, 0 to 1 part of vitamin B2 and 0 to 1 part of folic acid. The preparation method of the capsule preparation comprises the following steps: taking the raw materials according to the mass parts of the composition, and uniformly mixing to obtain an intermediate product; controlling the mass content of water in the intermediate product to be less than or equal to 5.0% And filling the intermediate product into a plant-based capsule shell. Human body trial feeding verifies that the composition can effectively improve skin cell metabolism, relieve inflammatory response, clear ROS and AGEs, inhibit melanogenesis, promote skin hydration and delay skin photoaging.

The invention discloses a composition for resisting photoaging and skin aging, and belongs to the field of skin care products. The composition comprises madecassoside, peony root bark extract, red ganoderma extract, sandalwood extract, solvent and other auxiliary materials. The red ganoderma extract, sandalwood extract, madecassoside and peony root bark extract used in the invention have unexpected synergistic antioxidant capacity, anti-wrinkle and other effects, can resist light pollution from the inside of the skin, reduce free radical formation, inhibit inflammation and erythema formation, and resist skin photoaging and skin aging.

The invention provides a medicine for treating skin photoaging. The medicine for treating skin photoaging comprises extracellular vesicles derived from adipose tissue. The medicine for treating skin photoaging provided by the invention can recover the activity of human dermal fibroblasts damaged by UVB, including improvement of cell proliferation and migration ability, stabilization of cell cycle, reduction of generation of reactive oxygen species (ROS) and reduction of expression of aging markers.

The invention discloses a traditional Chinese medicine composition for resisting skin photoaging and a preparation method and application of a compound preparation of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 70-90 parts of cornu cervi degelatinatum, 70-90 parts of radix angelicae sinensis, 70-90 parts of radix asparagi, 50-60 parts of rhizoma atractylodis macrocephalae, 50-60 parts of radix ampelopsis, 50-60 parts of rhizoma chuanxiong, 50-60 parts of semen armeniacae amarae, 50-60 parts of cassia twig and 50-60 parts of rhizoma zingiberis. Three medicinal materials including asarum, rhizoma typhonii and radix angelicae which are forbidden in cosmetics are removed from ancient prescription antler powder, on the basis of drug effect and property and flavor tropism, antler degelatinatum is used for replacing antler, three traditional Chinese medicines including rhizoma zingiberis, angelica sinensis and cassia twig are added, tedious operations such as soaking for a hundred days and grinding on stones in the ancient prescription are changed, and medicinal material components are obtained through an extraction method and prepared into a smearing preparation. The composition has a good protective effect in a skin photoaging model of middle-aged and aged mice, and especially has outstanding performance in the aspects of relieving wrinkle formation and collagen fiber damage caused by photoaging, relieving skin inflammation response and the like.

A method for preventing or delaying skin aging and maintaining or improving skin conditions. The method comprises: administering to a subject a composition comprising alitretinoin or a pharmaceutically acceptable salt, hydrate, ester, analog or derivative thereof. The method can effectively relieve skin photoaging, reduce fine lines and wrinkles on the skin, improve skin elasticity, skin firmness and skin glossiness, and whiten the skin and the like.

The invention discloses application of a rhizoma dioscoreae nipponicae saponin extract in preparation of medicines or cosmetics for preventing or treating skin photoaging, and belongs to the technical field of medicines. The rhizoma dioscoreae nipponicae saponin extract is extracted from rhizoma dioscoreae nipponicae by utilizing an alcohol extraction and water precipitation method, skin light aging is UVB-induced skin exogenous aging and skin injury, and experimental research proves that the rhizoma dioscoreae nipponicae saponin extract can relieve UVB-induced HaCaT cell viability and mitochondrial membrane potential drop and reduce SA-beta-Gal accumulation; meanwhile, the rhizoma dioscoreae nipponicae saponin extract can relieve UVB-induced content reduction of hyaluronic acid and hydroxyproline in C57BL / 6 mice and improve the pathological structure of skin tissues, so that the rhizoma dioscoreae nipponicae saponin extract can be used for preparing the medicines or cosmetics for preventing or treating skin photoaging, and has a very good application prospect.