3 results about "Autophagosome" patented technology

The application discloses oleanane triterpene saponin compounds with improved mitochondrial energy anti-aging, a preparation method and application thereof, and belongs to the technical field of medicines and cosmetics. Through systematic chemical component research on Zhejiang Hongshan tea flowers, two new oleanane triterpene saponin compounds CK and CL are separated, and the chemical structures are determined by using high-resolution mass spectrometry and nuclear magnetic technology. In-vitro anti-photoaging activity research shows that the new compounds CK and CL provided in the application have good protection effects on ultraviolet-induced fibroblast photoaging, can significantly improve the content of type I collagen, reduce the expression of matrix metalloproteinases mmp1 and mmp3 , increase the generation of mitochondrial autophagosomes and lysosomes, promote mitochondrial autophagy, relieve mitochondrial damage and improve cell senescence. The preparation method provided in the application is simple, and the activity is significant, so that the application is expected to be developed into a new anti-skin aging medicine or cosmetic.

This invention belongs to the field of biomedical technology, specifically relating to the application of piRNA-45962 inhibitors in the preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury. This invention discovers that piRNA-45962, with the nucleotide sequence shown in SEQ ID No. 1, is specifically highly expressed during myocardial ischemia-reperfusion. Furthermore, specific inhibitors of piRNA-45962 can improve the blockage of autophagic flux during myocardial ischemia-reperfusion through the HNRNPH1 / GATA5 signaling pathway, enhance the clearance of autophagosomes by cardiomyocytes, reduce cardiomyocyte apoptosis, and exert a preventive and therapeutic effect against myocardial ischemia / reperfusion injury. This invention provides a new solution for the prevention and treatment of myocardial ischemia-reperfusion injury and offers new ideas for the development of drugs for the prevention and treatment of myocardial ischemia-reperfusion injury, possessing promising application prospects and clinical value.

The present application relates to a kind of activation of cell autophagy Epimedium extract and its preparation method and application, the present application is prepared using specific fermentation bacteria and composite enzyme Epimedium extract, improve the content of Epimedium in Epimedium extract Epimedium secondary glycoside I, the Epimedium extract can be up-regulated lysosome mannose glycosidase (LManV) expression, significantly enhance cell autophagy flow, promote autophagosome and lysosome fusion, efficiently reverse the p62 / Ref (2) P accumulation caused by autophagy function decline, maintain intracellular environment steady, delay cell aging speed, provide support for organism anti-aging from cell level, help to improve the physical condition related to aging, maintain organism activity, and safe and nontoxic to human body.