Cloning and application of tomato ATG8f gene

Abstract

The invention discloses cloning and application of a tomato ATG8f gene. The gene plays an important role in a tomato autophagosome formation process. The tomato ATG8f gene is cloned, and an agrobacterium tumefaciens engineering bacterium A containing a tomato ATG8f gene over-expression vector is constructed; the agrobacterium tumefaciens engineering bacterium A is used for carrying out mediated transformation on an explant of a target plant to prepare an ATG8f gene transgenic plant; the vegetative growth of the plant is vigorous; the agrobacterium tumefaciens engineering bacterium A is infected with nicotiana benthamiana and a point-shaped autophagosome can be observed. The tomato ATG8f gene disclosed by the invention has important application value in cultivation of good tomato varieties and autophagosome detection.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to the cloning and application of tomato ATG8f gene. Background technique [0002] In recent years, my country's protected horticulture industry has developed rapidly, and the production area is still growing at a rate of about 10% per year. However, due to the simple structure of the facilities and backward facilities and equipment, the production of protected vegetables often encounters adversities such as low temperature and low light in winter and spring, and continuous cropping obstacles. The photosynthetic efficiency of tomatoes, cucumbers and other protected vegetables is significantly reduced, the pollen vigor is reduced, and the flower and fruit drop is serious, which in turn affects their yield and quality, and has become a bottleneck problem restricting the development of the protected vegetable industry. [0003] Tomato (Solanum lycopersicum L.) is a t...

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Problems solved by technology

[0002] In recent years, my country's protected horticulture industry has developed rapidly, and the production area is still growing at a rate of about 10% per year. However, due to the simple structure of the facilities and backward facilities and equipment, the production of protected vegetables often encounters adversities such as low temperature and low light in winter and spring, and continuous cropping obstacles. The photosynthetic efficiency of tomatoes, cucumbers and other protected vegetables is significantly reduced, the pollen vigor is reduced, and the flowers and fruits are seriously dropped, which in turn affects their yield and quality, and has become a bottleneck problem restricting the development of the protected vegetable industry.

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