1187 results about "Agrobacterium" patented technology

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention provides a Chinese cabbage clubroot susceptibility related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to a Chinese cabbage clubroot susceptibility related gene BrSWEET14a. The CDS sequence of the Chinese cabbage BrSWEET14a is as shown in SEQ ID No. 1 (sequence identifier number 1). The gene is transformed into Col-type arabidopsis thaliana through an agrobacterium flower immersion transformation method, a BrSWEET14a heterologous expression arabidopsis thaliana strain is obtained, and the result shows that T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to clubroot than that of the Col-type arabidopsis thaliana, and heterologous expression of Chinese cabbage BrSWEET14a leads to reduction of disease resistance of arabidopsis thaliana to plasmodiophora brassicae. Therefore, the Chinese cabbage BrSWEET14a is closely related to the clubroot disease, and the gene and the homologous gene of the gene in other cruciferous plants can be applied to breeding of the cruciferous plants and other horticultural plants and have a good application prospect.

The invention discloses a pennisetum purpureum gene PpbHLH148L and application thereof in improving the cold resistance of plants, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. After the gene is subjected to subcellular localization and expression condition analysis after cold stress, the gene is related to low-temperature tolerance. An overexpression vector of the gene is constructed, Agrobacterium tumefaciens is transferred, then Arabidopsis thaliana infection is carried out, cold tolerance determination is carried out on overexpressed Arabidopsis thaliana, phenotypic difference and physiological index difference between an overexpressed strain after cold treatment and a wild type are observed, and it is found that the gene can significantly improve the low-temperature stress tolerance of Arabidopsis thaliana. And in the low-temperature treatment of the pennisetum alopecuroides transgenic callus, the transgenic callus also shows higher tolerance than the wild callus at low temperature. The pennisetum purpureum gene PpbHLH148L can be used for low-temperature tolerance modification of plants, and has an application prospect in creation of low-temperature-resistant plant materials.

The invention belongs to the technical field of fungal gene engineering, and discloses a shiitake mushroom ABC transporter gene LeABC2 and application thereof in regulation and control of shiitake mushroom heat resistance. Whole genome association analysis is carried out based on heat resistance phenotype data of 133 shiitake mushroom strains, a candidate gene LeABC2 for regulating heat resistance is obtained, a LeABC2 gene RNA interference vector is constructed, the gene function is researched through an agrobacterium tumefaciens-mediated genetic transformation method, and the result shows that the LeABC2 gene negatively regulates the heat resistance of shiitake mushroom hyphae, the heat resistance of the hyphae can be improved by interfering the expression of the gene, and the gene has a good application prospect. A new solution thought is provided for breaking through the problem that the yield and quality are influenced by high temperature in the mushroom industry.

The invention discloses an endogenous tRNA (transfer ribonucleic acid) promoter. The nucleotide sequence of the endogenous tRNA promoter is shown as SEQ ID NO: 1; an expression cassette containing the endogenous tRNA promoter and a recombinant vector; the invention also relates to application of the endogenous tRNA promoter, the expression cassette or the recombinant vector in thraustochytriaceae microorganism gene editing, and a thraustochytriaceae microorganism gene editing method. The CRISPR / Cas9 gene editing system based on the endogenous tRNA promoter is successfully established, convenient, rapid and efficient fixed-point editing in thraustochytriaceae microorganisms is achieved, and the CRISPR / Cas9 gene editing system has high application value; a transformation system for screening the recombinant thraustochytriaceae microorganisms by using sodium acetate as an auxotroph is established, the transformation screening efficiency of the recombinant thraustochytriaceae microorganisms obtained based on an agrobacterium transformation method is remarkably improved, and rapid gene editing is assisted.

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention is applicable to the technical field of biotechnology and genetic engineering, and provides an agrobacterium rhizogenes mediated genetic transformation method for hairy roots of blueberries. According to the method, a blueberry perennial stem segment is used as a receptor material for infection, and rapid genetic transformation of the blueberry stem segment is realized through an agrobacterium tumefaciens-mediated method. The method ingeniously bypasses the lengthy process of differentiating the explants to form somatic embryos, and compared with a traditional leaf disc method genetic transformation system, the method has the advantage that the genetic transformation period is remarkably shortened. And the method can be used for mass infection. Even in a non-sterile environment, the method can realize high-efficiency transformation of the gene, and shows the characteristics of simplicity and high efficiency. Therefore, the invention not only provides a new way for genetic transformation of the blueberries, but also provides valuable reference for genetic transformation research and gene function research of the blueberries.

The invention discloses an application of a rape BnC05ERF378 gene in improving the waterlogging resistance of a plant. The nucleotide sequence of the rape BnC05ERF378 gene is as shown in SEQ ID NO. 1. According to the invention, a brand new stain-resistant gene BnC05ERF378 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of the transgenic BnC05ERF378 gene arabidopsis thaliana is remarkably superior to that of a wild type after waterlogging treatment, the transgenic BnC05ERF378 gene arabidopsis thaliana can normally bolt, the survival rate is increased from 12.5% to 37.5%, it is fully proved that the gene can enhance the survivability of the plant in a waterlogging environment by regulating the response mechanism of the plant to waterlogging stress, and the survival rate of the plant is increased from 12.5% to 37.5%. And a key guarantee is provided for stable growth of plants under high-humidity, waterflooding and other adverse conditions. According to the discovery and application of the waterlogging-resistant gene BnC05ERF378 disclosed by the invention, a clear functional gene target is provided for improvement of waterlogging resistance of crops. By constructing an expression vector containing the gene and combining mature technologies such as agrobacterium transformation, the gene can be stably introduced into a target plant, and a pure transgenic plant is obtained.

Disclosed is an innovative method for genetic transformation through injection of Agrobacterium containing plasmid DNA expressing PLETHORAs (PLT5) or / and WOUNDINDUCED DEDIFFERENTIATION 1 (WIND1) in multiple plant species including Snapdragon and tomato. In addition, this plasmid has also been tested that it can significantly improve the genetic transformation method is not genotype dependent. Genetic transformation can be achieved by promoting de-novo regeneration of shoots in adult plants, which will of significant impact on some perennial plant species such as citrus and blue berry etc. The transgenic de-novo shoot regenerated from adult plants can by-pass the juvenile phase of plants regenerated through the tissue culture method. Expression of PLT5, WIND1, or both can also greatly improve plant regeneration and transgenic transformation through in-vitro tissue culture in recalcitrant plant species.

The invention discloses application of a PagBAG8 gene in regulation and control of poplar growth and development, and belongs to the technical field of forestry biological genetic engineering. According to the application of the PagBAG8 gene in regulating and controlling growth and development of the poplar, the nucleotide sequence of the PagBAG8 gene is shown as SEQ ID NO: 1, and the method for cultivating the transgenic poplar through the PagBAG8 gene comprises the following steps that the PagBAG8 gene of the poplar is cloned; the PagBAG8 gene is connected to a vector, and an overexpression vector is obtained; transforming the obtained overexpression vector into agrobacterium to obtain an agrobacterium liquid; and infecting poplar leaves with the obtained agrobacterium liquid to obtain a PagBAG8 gene overexpression plant. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a potential target for molecular breeding improvement of the properties of the poplar.

The present invention provides L-β-galactoglucan, a preparation method therefor, and a use thereof. The polysaccharide is sinistrin, is composed of β-D-glucose and β-D-galactose, and has a molecular weight of not less than 5000 Da. The preparation method for the polysaccharide comprises: inoculating Agrobacterium FN01 in a culture solution for fermentation to obtain a fermentation broth; mixing the fermentation broth with alcohol, and carrying out precipitation and drying to obtain a crude polysaccharide product; and then carrying out purification to obtain a purified polysaccharide product, or carrying out enzymolysis on the fermentation broth by using glucanase, and then carrying out filtration, ultrafiltration concentration, and spray-drying to obtain a purified polysaccharide product. The polysaccharide has the effects of treating and / or preventing liver injury, resisting aging, decreasing blood sugar, repairing tissues and the like, and is a multipurpose polysaccharide.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The invention discloses application of a transcription factor CgGATA8 in adjusting lignin synthesis, and belongs to the technical field of genetic engineering breeding and fruit and vegetable preservation. According to the invention, the transcription factor CgGATA8 is instantaneously transferred into the juice sac of the Majia pomelo through an agrobacterium infection method, the lignin content in the over-expressed juice sac is obviously higher than that in no-load control, and autofluorescence signals are enhanced, secondary cell walls are thickened and the juice sac granulation symptom is aggravated in phloroglucinol dyeing and paraffin section at the same time. Molecular biology function verification shows that the Marijia pomelo transcription factor CgGATA8 can be combined with and activate a CgPOD16 promoter, the expression level of the CgPOD16 gene is improved, and the accumulation amount of lignin synthesis in fruits is improved, so that the CgGATA8 gene participates in regulation and control of synthesis and deposition of lignin in Marijia pomelo fruit juice cells, and has the function of promoting maturity and aging of picked fruits.

The invention discloses an application of a CsHCT1 gene in increasing the content of citrus flavonoids, belongs to the technical field of agricultural biological genetic engineering, and provides an application of the CsHCT1 gene in increasing the content of citrus flavonoids, and the nucleotide sequence of the CsHCT1 gene is as shown in SEQ ID NO.1. The invention further discloses an application of the CsHCT1 gene in increasing the content of citrus flavonoids. The invention also discloses a method for improving the flavone compound of the citrus peel by using the CsHCT1 gene. The method comprises the following steps: cloning the citrus CsHCT1 gene; a CsHCT1 overexpression vector is constructed; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and injecting the CsHCT1 gene into the citrus fruit to obtain the CsHCT1 gene overexpressed citrus fruit. A new choice is provided for increasing the flavonoid compounds in the citrus peel, the content of vicenin-2 and / or hesperidin in the citrus peel is effectively increased, and the application has great application value for cultivating new functional citrus varieties and accelerating the breeding process.

The invention relates to a gene editing vector of agrobacterium, a gene editing method of the gene editing vector and an agrobacterium editing strain. The agrobacterium gene editing vector comprises the following gene elements: a plasmid skeleton, a Pvan promoter, a spCas9 gene, a green fluorescent protein gene GFP, gRNA, and an upstream homologous arm and a downstream homologous arm of a target gene. According to the agrobacterium gene editing vector, a CRISPR / spCas9-GFP gene editing system is adopted, an efficient, simple, convenient and traceless genome editing system is constructed in agrobacterium, and gene knockout and insertion can be achieved.

The invention discloses a rice salt stress resistant gene OsHAK11 coding protein and application thereof, and belongs to the field of plant genetic engineering. According to the method, a rice OsHAK11 gene (the nucleotide sequence is shown as SEQ ID NO.1) is knocked out through a CRISPR / Cas9 gene editing technology, and a mutant with significantly enhanced salt tolerance is obtained. The gene editing vector pEGCas9Pubi-B-OsHAK11 contains sgRNA of a region as shown in a target SEQ ID NO.4, a rice receptor material is transformed through agrobacterium tumefaciens mediation, and a plant with the OsHAK11 gene subjected to frame shift mutation is obtained through screening. A salt stress experiment shows that the survival rate of the mutant oshak11 is obviously higher than that of a wild type. The invention provides a new gene resource and an efficient technical means for salt-tolerant breeding of rice.

The invention discloses a method for improving the inductivity and genetic transformation efficiency of hairy roots of toona ciliata. According to the method, agrobacterium rhizogenes carrying RUBY-TcGRF8-GIF2 chimeric protein are injected into stems and petioles of aseptic seedlings of the toona ciliata, and the TcGRF8-GIF2 chimeric gene is over-expressed, so that the induction efficiency and the genetic transformation efficiency of the toona ciliata hairy roots are remarkably improved, and the transformation period of the toona ciliata hairy roots is remarkably shortened. Specifically, after overexpression of the TcGRF8-GIF2 gene, the induction time of the hairy roots is shortened from 70 days of a RUBY-XbaI no-load plasmid control group to 25 days, the induction rate and conversion rate of the hairy roots mediated by agrobacterium tumefaciens are improved, the operation efficiency of genetic transformation of the toona ciliata is effectively improved, and the method has important significance for promoting genetic improvement of the toona ciliata.

The invention discloses a watermelon transgenic screening expression cassette and application thereof, and belongs to the technical field of biology. According to the invention, an expression cassette of a TagRFP gene driven by a d35s promoter is constructed on a pCambia1300 vector, and a recombinant vector is obtained. A recombinant vector is introduced into watermelon cells by utilizing an agrobacterium-mediated genetic transformation technology, and a red fluorescent protein TagRFP gene is integrated into a watermelon genome, so that transgenic watermelons can be screened and identified by taking red fluorescence as an identifier in the whole growth period of watermelon genetic transformation. Compared with a traditional screening method, the screening method has the advantages that the screening process is greatly simplified, and the transgenic watermelon and the non-transgenic watermelon are clearly distinguished, so that the genetic transformation efficiency of the watermelon is remarkably improved.

The invention provides an SlTAGL1 gene for regulating and controlling the lutein content of tomato fruits, a method and application, a coding sequence of the SlTAGL1 gene is obtained from a tomato genome, a target spot is connected to CRISPR / Cas9 by designing the target spot, the tomato is successfully infected by using agrobacterium competent cells, a CRISPR / Cas9 gene editing plant of the tomato is obtained, the gene editing plant is analyzed, and the content of lutein in the tomato fruits is regulated and controlled. The result shows that compared with the wild tomato, the lutein content in the tomato fruits after the SlTAGL1 is knocked out is obviously increased by 1.6-2.2 times. According to the invention, an efficient and stable genetic method is adopted to improve the content of xanthophyll in tomato fruits, so that the quality of the tomato fruits is improved, and gene resources and strategies are provided for culturing high-xanthophyll tomato varieties.

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention relates to the field of rice gene engineering, and discloses an application of a GSE3.1 protein and a coding gene thereof in regulating and controlling the grain length, the grain width and the grain weight of rice seeds. According to the invention, a GSE3.1 gene is edited based on a CRISPR / Cas9 technology, a japonica rice variety Zhonghua 11 (ZH11) is introduced by using an agrobacterium-mediated method, and a knockout mutant is obtained through screening. The homozygous knockout mutation of the GSE3.1 gene in the invention leads to reduction of the grain width and grain length of rice seeds and reduction of thousand grain weight. According to the invention, the constructed plant overexpression vector ProActin: GSE3.1 is expressed in wild type ZH11, so that compared with the wild type plant, the seed grain length and grain width of the transgenic plant are obviously increased, and the thousand grain weight is obviously increased. Therefore, the GSE3.1 gene and the encoding protein thereof can regulate and control the size and the weight of the rice seeds, and are of great significance to cultivation of high-yield rice varieties.

The invention provides an apple laccase LAC3 gene and application thereof in improving aphid resistance of plants, the apple laccase LAC3 gene is cloned, a nucleotide sequence and a protein structure of the apple laccase LAC3 gene are clarified, a primer is designed on the basis, an overexpression vector of the apple laccase LAC3 gene is constructed, and the overexpression vector is transferred into tobacco through tobacco tissue culture seedling culture and agrobacterium mediation to obtain a transgenic plant. Homozygote strains are obtained through screening, aphid feeding behavioral observation is carried out through an EPG technology, aphid inoculation research is carried out, and the aphid resistance function of the aphid strains is clarified. Compared with a wild plant, the growth vigor density of the overground part of the transgenic plant is larger than that of the wild plant, stalks are thick and strong, lignin is increased, a good resistance effect on aphids is achieved, and a theoretical basis is provided for aphid-resistant molecule improved breeding.

The invention relates to the field of rice gene engineering, and discloses an application of OsMYB36 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsMYB36 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsMYB36 gene knocked out is remarkably higher than that of a wild-type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsMYB36 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a gene SbAIF1 for enhancing sorghum alkali tolerance and application of the gene SbAIF1. According to the invention, the gene SbAIF1 is separated and cloned from sorghum, the CDS sequence of the SbAIF1 gene is connected with pCAMBIA2300-GFP-BWM, and the sorghum is transformed by using an agrobacterium-mediated method. Experimental results show that the SbAIF1 gene is over-expressed in sorghum, the alkali tolerance of the sorghum is enhanced after the gene is over-expressed, meanwhile, the survival rate of the sorghum under alkali stress can be increased after the gene is over-expressed, and a new thought is provided for cultivating saline-alkaline tolerant transgenic plants.

The invention discloses an application of an OsATS3 gene in regulation and control of rice flooding stress tolerance. According to the invention, an OsATS3 gene is constructed to edit a knockout vector, an agrobacterium tumefaciens-mediated method is adopted to transform rice calluses, and a knockout strain of the OsATS3 gene is obtained through screening, and the knockout strain of the OsATS3 gene shows a phenotype that after seeds germinate under a waterflooding condition, seedling root systems are developed, seedling growth of overground parts of seedlings is faster, the growth speed of first leaves is faster, and the seedling rate is high; the method has great significance in solving the problems of slow seedling growth, inhibited radicle development, low seedling rate and the like in the rice direct seeding process, and has a wide application prospect in plant breeding.

The invention relates to the technical field of plant genetic engineering, in particular to a genetic transformation method of prunus mume line plum blossom leaf calluses. The method comprises the following steps: firstly preparing a plum blossom genetic transformation receptor, then transforming agrobacterium tumefaciens by taking an RUBY gene as a reporter gene to prepare an infection bacterium solution, then carrying out callus infection and co-culture, and finally screening to obtain the resistant callus. According to the method provided by the invention, the resistant callus can be identified in an observation or PCR (Polymerase Chain Reaction) detection mode. The plum blossom callus is successfully infected, the red callus expressed by the RUBY reporter gene is obtained, the inductivity is high, and a good basis is provided for establishment and genetic improvement of a plum blossom genetic system.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of oil content and lignin content of rape seeds. The amino acid sequence of the transcription factor BnaMYB52 is shown as SEQ ID NO.5, or other amino acid sequences which are derived from the amino acid sequence shown as SEQ ID NO.5 through substitution and / or deletion and / or addition of one or more amino acid residues and have the same protein function. An agrobacterium tumefaciens-mediated genetic transformation method is used for performing overexpression and gene editing on the BnaMYB52 gene respectively, and it is found that the BnaMYB52 gene negatively regulates and controls the oil content of rape seeds and positively regulates and controls the lignin content. The method disclosed by the invention has very important application prospects in high-oil-content breeding of the rape and quality improvement of the rape cake meal.