26 results about "Pleurotus" patented technology

The utility model belongs to the field of mushroom greenhouse planting equipment, and particularly relates to a pleurotus nebrodensis full-automatic adjusting growth shed which comprises a shed body and planting net racks, the planting net racks are arranged in the shed body at intervals, a picking channel is formed between every two adjacent planting net racks, and a light supplementing unit is arranged in the shed body. The light supplementing units are arranged on the planting net racks on the two sides of the picking channel, mushroom sticks are arranged on the side walls, close to the picking channel, of the planting net racks, and the light supplementing units irradiate the mushroom sticks on the adjacent planting net racks. The distance between the planting net racks can be shortened, and the utilization rate of the space in the greenhouse is increased.

The utility model belongs to the field of straw smashing equipment, and particularly relates to a corn straw smashing machine for oyster mushroom planting, which comprises a rack, a driving motor, a smashing mechanism, a discharging mechanism and a spraying mechanism, the smashing mechanism and the discharging mechanism are communicated and arranged on the rack, the discharging mechanism comprises a discharging bin and a conveying belt, the discharging bin is obliquely arranged, and the conveying belt is arranged on the rack. The conveying belt is arranged in the discharging bin, the driving motor is arranged at the bottom of the rack, the driving motor is in transmission connection with the crushing mechanism, the driving motor is in transmission connection with the conveying belt, the spraying mechanism comprises an atomizing nozzle, a sealing cover, a water pump and a water tank, a through hole is formed in the top wall of the discharging bin, the sealing cover is installed on the through hole, and the water pump is installed on the water tank. The atomizing nozzle is installed in the sealing cover, the water pump and the water tank are located at the bottom of the machine frame, and the atomizing nozzle is communicated with the water tank through the water pump. According to the corn straw smashing device, auxiliary materials can be added while corn straw is smashed, and the processing speed of the corn straw is increased.

The present invention relates to the field of active ingredient preparation, and more particularly to a method for preparing and applying a ceramide derived from the golden oyster mushroom (Pleurotus citrinipileatus), possessing moisturizing, brightening, anti-aging, and skin barrier-maintaining properties. The method comprises the following steps: collection and pretreatment; fermentation treatment; separation and extraction; and refining. The advantage lies in the fact that the method for preparing and applying this ceramide, through the optimization of fermentation conditions and separation and extraction techniques, allows for the efficient preparation of the ceramide from the golden oyster mushroom (Pleurotus citrinipileatus) and increases the yield and purity of the ceramide.

The invention discloses a method for preparing an oyster mushroom cultivation auxiliary material by using dining-table remaining food and the oyster mushroom cultivation auxiliary material, and relates to the technical field of dining-table remaining food recycling, the method comprises the following steps: S1, pretreatment: through multiple oil and salt removal treatment steps, the oil and salt removal efficiency of garbage materials is high; s2, sterilization and separation: performing high-efficiency sterilization'harm removal and retention 'to further adapt to a subsequent fermentation process, and performing centrifugal high-efficiency separation on a required solid phase; s3, fermentation: converting a solid-phase protein part into small-molecular protein in a microbial fermentation manner to achieve efficient protein conversion; and S4, post-processing. The biological compound protein material obtained by the preparation method provided by the invention is clear in yield increasing mechanism, and can provide higher contents of crude protein, cellulose, lignin and crude fat in the cultivation material, and the obtained product can equivalently replace traditional bran, corn flour and soybean meal to be used for oyster mushroom strong hypha yield increasing cultivation, so that recycling and high-value utilization of recycled wastes are realized.

The invention relates to the field of large-scale pretreatment of edible mushrooms, in particular to ingredient mixing and stir-frying equipment for producing pleurotus geesteranus biscuits, which comprises a rack, an obliquely arranged pot body, a planetary stirring module, an integrated intelligent airflow distribution module and an intelligent control system. The pot body is inclined; the planetary stirring module comprises a revolution bracket, a revolution stirrer and at least four autorotation stirrers, realizes composite stirring through linkage of an eccentric slide rail and a connecting rod, and covers the whole cavity without dead angles; the integrated intelligent gas flow distribution module comprises three independent gas flow distribution modules, the composite function distribution plate is formed by brazing a liquid seepage layer, a flow channel isolation layer and a gas distribution layer, and gas-liquid collaborative atomization is achieved. The control system is accurately linked with all the modules, and it is ensured that mixing, heating and gas-liquid action are orderly coordinated. According to the equipment, integrated pretreatment of'scattering, mixing, heating and tempering 'of pleurotus geesteranus is realized, the pretreatment uniformity and suitability of the pleurotus geesteranus are greatly improved, and core guarantee is provided for the quality of finished pleurotus geesteranus biscuits.

The invention provides selenized pleurotus geesteranus polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, extracting pleurotus geesteranus polysaccharide from pleurotus geesteranus; 2, the pleurotus geesteranus polysaccharide reacts with Na2SeO3 and nitric acid, then alcohol precipitation is conducted, and the selenized pleurotus geesteranus polysaccharide is obtained. The selenium element can be successfully introduced into the pleurotus geesteranus polysaccharide structure through selenylation modification, the sulfate content and the selenium loading capacity of the polysaccharide are remarkably improved, and the content of total polysaccharide and the content of total protein are not subjected to obvious negative effects. The obtained selenized pleurotus geesteranus polysaccharide has excellent antioxidant activity and blood fat reducing activity at the same time, can be used as a functional additive, and has a good application prospect in blood fat reducing and antioxidant health-care food and medical products.

The utility model provides a kind of picking device for Pleurotus geesterus, including mobile base, folding ladder, storage rack, the mobile base is equipped with movable slot, the folding ladder is by first ladder, second ladder is constituted, the top hinged of first ladder, second ladder, the bottom end both sides of first ladder are rotated hinged in movable slot, the bottom of second ladder is rotatably connected with a sliding frame, and the both ends of sliding frame are slidably connected with movable slot, first electric telescopic rod is installed between the bottom of first ladder and second ladder, and the telescopic end of first electric telescopic rod is connected with the sliding frame of the bottom of second ladder, second electric telescopic rod is installed on the side of folding ladder in mobile base, and storage rack is installed on the top end of second electric telescopic rod. The utility model has the advantages of convenient movement, reducing labor intensity etc.

The present application belongs to the technical field of edible mushroom cultivation, and particularly relates to a cultivation method of Pleurotus, comprising the following steps: S1 fermentation: corn stalks are chopped to 2-3 cm, lignocellulose, wheat bran, tea seed meal, gypsum and water are added and mixed uniformly, then a compound microbial inoculum is added, and dry materials are obtained after fermentation; S2 ingredient preparation: trehalose, malt dextrin, calcium phosphate, sodium chloride and citric acid are dissolved in water, and then poured into the dry materials, mixed uniformly, and a cultivation substrate is obtained; S3 sterilization, inoculation and bacteria cultivation; S4 mushroom management; and S5 harvesting; the cultivation method of Pleurotus provided by the present application mainly uses corn stalks as the substrate raw material, which not only effectively reduces the use of sawdust in the cultivation process, reduces the production cost and the harm to the environment, but also improves the quality and nutrient content of Pleurotus, is suitable for large-scale promotion, and has significant economic and social benefits.

The invention discloses an intelligent oyster mushroom growth environment adjusting and optimizing system combined with spectral sensitivity. The system comprises a multi-dimensional data acquisition layer, a spectrum analysis layer and a spectrum analysis layer, wherein the multi-dimensional data acquisition layer is used for acquiring environmental parameters and multi-period spectrum data in the oyster mushroom growth process; the intelligent preprocessing layer is used for preprocessing the collected spectral data and standardizing environmental parameters; the feature engineering layer is used for extracting key spectral features from the preprocessed spectral data and fusing the key spectral features with the standardized environmental parameters to construct a multi-dimensional feature matrix; the model prediction layer is used for predicting the protein content of the oyster mushrooms by utilizing an integrated machine learning model based on the multi-dimensional feature matrix; and the intelligent regulation and control layer is used for dynamically adjusting the environment parameters and the illumination conditions of oyster mushroom growth based on the prediction result, and the protein content dynamic prediction and the growth environment adaptive adjustment and optimization based on the fusion analysis of the oyster mushroom growth real-time spectral characteristics and the environment parameters are realized. The accuracy, the automation level and the production efficiency of oyster mushroom quality cultivation are fundamentally improved.

The invention provides a primer and a method for detecting the expression characteristic of a papaya Pleurotus geesteranus transaminase gene. The sequences of the primers are as shown in SEQ ID NO. 1-2. Based on the primer, the invention establishes a fluorescent quantitative PCR method for detecting the change of the expression quantity of the transaminase gene after the papaya Pleurotus geesteranus eats two different hosts, namely papaya and potato. Through transcriptome sequencing verification, the gene expression trend obtained by the fluorescent quantitative PCR is consistent with the transcriptome analysis result. The invention lays an important foundation for exploring the influence of host conversion on the defense mechanism and transaminase gene expression characteristics of the papaya Pleurotus geesteranus.

This invention discloses a strain of *Pleurotus ostreatus* and its application in the degradation of mycotoxins. The strain exhibits the ability to degrade various mycotoxins (including OTA, ZEN, AFB1, and DON). The freeze-dried fruiting body powder and mycelium powder extract of this strain show a degradation efficiency of up to 99% for OTA (1 μg / mL), and the mycelium extract achieves near-complete removal of OTA within 6 hours. The fruiting bodies of the *Pleurotus ostreatus* strain 914T1 of this invention are characterized by high water content and tender texture. Their dry matter is rich in total sugar, protein, and cellulose, expanding the scope of edible fungi in the field of mycotoxin degradation and providing new strain resources for the development of related biological agents.

The invention relates to a pleurotus geesteranus strain L6885 and an artificial cultivation method and application thereof, and belongs to the technical field of biology. The pleurotus geesteranus strain L6885 is preserved in the Guangdong Microbial Culture Collection Center in China on May 27, 2025, and the preservation number is GDMCC No: 66423. The fruiting body form of the pleurotus geesteranus strain L6885 is monophytic or clustered, pileus is flat hemispherical to flat, inverted oval or nearly fan-shaped, the expanding period is taupe, the mature period is off-white, most of pleurotus geesteranus varieties in the market are taupe or dark gray, and compared with the conventional pleurotus geesteranus varieties in the market, the pleurotus geesteranus strain L6885 is richer in fragrance, whiter in color and tender in mushroom meat. The pleurotus geesteranus has the advantages of neat fruiting, high yield, high content of crude polysaccharide and calcium and the like, and is suitable for large-scale factory-like demonstration, popularization and planting.

The application discloses a method for reducing cadmium enrichment and improving magnesium content in ZJXZG001 under cadmium stress environment, and relates to the technical field of microbial fermentation, which comprises the following steps: inoculating ZJXZG001 into a liquid medium added with a water-soluble cadmium-containing compound after activation, and then carrying out fermentation culture after adding GABA in the liquid medium to obtain a fermentation product (containing fermentation liquor and mycelium), and filtering the fermentation liquor to obtain the mycelium; the added amount of GABA in the liquid medium is 10-25 mg / L; and the preservation number of the ZJXZG001 strain is CGMCC NO.40780. Pleurotus pulmonarius By adding GABA with a proper concentration under the condition of exogenous cadmium stress, the inhibitory effect of cadmium on the growth of the mycelium of ZJXZG001 can be effectively relieved, the enrichment amount of cadmium in the mycelium can be significantly reduced, the content of magnesium in the mycelium can be greatly improved, and the regulation effect of "one dose with multiple effects" can be realized. The method is suitable for the fermentation production of ZJXZG001 under the environment polluted by cadmium, and has good application prospect and industrialization value.

This invention provides the application of *Pleurotus oosporum* polysaccharide in the preparation of drugs and health foods that regulate intestinal barrier function or protect against intestinal damage-related diseases. Its protective effect against a mouse model of intestinal damage induced by sodium dextran sulfate was investigated. Results showed that *Pleurotus oosporum* polysaccharide significantly improved symptoms such as weight loss, loose stools, and colonic atrophy caused by intestinal damage in mice, reduced the disease activity index, inhibited colonic epithelial destruction, inflammatory cell infiltration, and goblet cell necrosis, and enhanced intestinal mucosal barrier function. As a fungus, *Pleurotus oosporum* has high safety and is a potential source of active ingredients against intestinal damage, showing promising development prospects in intestinal barrier protection.

This invention discloses a strain of *Pleurotus erythrorhizon* and its application in the degradation of polyurethane plastics. A strain of *Pleurotus erythrorhizon*, SC23, capable of degrading polyurethane foam, has the accession number GDMCC No: 63578. The *Pleurotus erythrorhizon* SC23 described in this invention can grow using Impranil DLN as the sole carbon source, exhibiting broad environmental adaptability and plastic degradation potential. This strain achieves a degradation efficiency of 94.17% for 6 g / L polyurethane foam within 20 days and a degradation efficiency of 37.86% for 1 g / L agricultural mulch film (PBAT) within 30 days. After degradation by the strain, the volume of the plastic is significantly reduced, and the plastic surface is eroded. Therefore, this invention provides an important biological resource for the biological treatment of waste polyurethane plastics and agricultural mulch film.

This invention belongs to the field of oyster mushroom cultivation substrate technology, specifically relating to a composite substrate formula for oyster mushroom cultivation that utilizes the resource-based utilization of *Pinus massoniana* medicinal residue. The formula, by weight, consists of the following raw materials: 10-60 parts *Pinus massoniana* medicinal residue, 20-70 parts cottonseed hulls, 15-19 parts wheat bran, 1-3 parts sucrose, and 0.5-1.5 parts gypsum powder. This invention replaces an equal amount of cottonseed hulls with *Pinus massoniana* medicinal residue, reducing oyster mushroom cultivation costs and solving the problem of *Pinus massoniana* medicinal residue waste disposal, thus realizing the resource utilization of waste. Furthermore, experiments show that when the addition ratio of *Pinus massoniana* medicinal residue is 10%, it does not substantially inhibit normal mycelial growth; mycelial vitality is well maintained. It not only does not cause yield reduction but also promotes the formation and accumulation of oyster mushroom fruiting bodies, increasing oyster mushroom yield, improving the appearance and quality of oyster mushroom fruiting bodies, and enhancing their marketability. Simultaneously, it also improves the nutritional quality of oyster mushrooms, which is of great significance for promoting the healthy development of the oyster mushroom cultivation industry.

The invention discloses application of edible mushrooms and associated trichoderma thereof in synergistic inhibition of aspergillus flavus, and belongs to the technical field of microorganisms. According to the method, a trichoderma library with antagonistic ability is separated from the waste mushroom substrate through a three-stage screening system, two strains of trichoderma associated with pleurotus geesteranus have an outstanding aspergillus flavus antagonistic effect, and growth and toxin production of aspergillus flavus can be more efficiently inhibited under the cooperation of pleurotus geesteranus. Furthermore, a synergistic system of pleurotus geesteranus and associated trichoderma can effectively prevent and control aflatoxin B1 pollution loss caused by aspergillus flavus infection in peanuts, and has the effect of promoting peanut growth. According to the method, the waste edible mushroom substrate can be recycled, so that resource utilization of agricultural wastes is realized, and the blank in the field of biological detoxification can be filled up, so that the input-output ratio of agriculture is increased. Therefore, the method has a good application prospect.

The application discloses a cultivation method of pleurotus geesterus and a culture medium thereof, and belongs to the technical field of cultivation of pleurotus geesterus. The application selects ginger stalks as culture materials for cultivation of pleurotus geesterus, so that part of cotton seed hulls in the original culture medium raw materials can be replaced, the amount of cotton seed hulls is reduced, the cost is lowered, and the comprehensive quality of the pleurotus geesterus is positively affected. The circulation and cooling mode promotes the growth of the pleurotus geesterus and reduces the occurrence of the pleurotus geesterus yellow spot disease. In the process of fruiting, different film covering modes are adopted according to the humidity and ventilation requirements of the pleurotus geesterus. In the early stage of fruiting, a double-layer covering mode of a perforated plastic film and non-woven fabric is adopted to ensure the humidity of the surface of the fungus bag. With the growth of the mushroom body, a large amount of oxygen is consumed and carbon dioxide is generated. At this time, the non-woven fabric is removed, and only the perforated plastic film is reserved. The perforated plastic film has certain air permeability, avoids the fungus bag from being in a hot and humid environment with high concentration of CO2 for a long time, and reduces the occurrence of the fruit body yellow spot disease.

The present application relates to the field of mushroom planting, in particular to a production and planting method of pleurotus nebroseus, mainly comprising the following steps: S1: preparing a culture solution, sterilizing the culture solution, planting pleurotus nebroseus spores in the sterilized culture solution, and preparing into mycelium ball solution; the culture solution comprises a carbon source, a nitrogen source, water and inorganic salt; the carbon source is at least one of potato juice and sucrose; the nitrogen source is at least one of bran powder and ammonium nitrate; S2: spraying the mycelium ball solution prepared in step S1 in a solid culture medium to prepare a pre-culture medium; S3: culturing the pre-culture medium prepared in step S2 to grow fungi, and then picking and packaging to obtain the product. The present application has the advantages of improving the fruiting rate of pleurotus nebroseus cultivation process and the growth quality of single mushroom.

The invention discloses a straw feed liquid fermentation method and application thereof, and the method comprises the following steps: taking pleurotus geesteranus as a fermentation strain, preparing a seed culture medium containing inducer paper pulp by utilizing natural high cellulase activity of the pleurotus geesteranus, sterilizing, inoculating and culturing to obtain a seed solution with high cellulase activity; and inoculating the pleurotus geesteranus thallus into a straw liquid fermentation culture medium for fermentation culture, carrying out graded filtration on the fermentation liquid, and drying pleurotus geesteranus thallus solid residues to obtain the liquid fermentation straw feed. According to the method, the inducer paper pulp is added in the culture process of the seed culture medium, so that the straw utilization rate is increased by 25%, meanwhile, the enzyme activity is remarkably improved, the protein content of a fermentation product reaches 40% or above, the polysaccharide content of edible fungi reaches about 8%, and the fermentation period is shortened to 4 days. Compared with other liquid-state fermented straw feed, the liquid-state fermented straw feed has higher application value and popularization prospect, is suitable for large-scale production of high-value straw feed, effectively reduces the production cost of the straw feed, and assists green agriculture development.

This invention discloses a multifunctional stem-coating medicated fertilizer solution, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The multifunctional stem-coating medicated fertilizer solution of this invention comprises the following raw materials: wood vinegar, microbial inoculants, earthworm protein peptides, *Alternaria solani* extract, potassium humate, lecithin, sodium carboxymethyl cellulose, chitin, urea, potassium dihydrogen phosphate, a chelated trace element mixture, and water. The microbial inoculants include *Bacillus laterosporus*, *Burkholderia ulmoides*, and *Trichoderma pleurotus*. This invention, through the scientific compounding of three specific strains, works synergistically with the other raw materials in the formula, solving the problems of limited disease resistance and low fertilizer utilization rates in existing apple tree stem-coating agents. While effectively controlling apple tree rot, it also significantly promotes fruit enlargement, increases yield and quality, improves tree vigor, and is environmentally friendly, with no adverse effects on the environment or fruit, showing broad application prospects.

The invention discloses an efficient pleurotus geesteranus fruiting management method, and relates to the technical field of edible fungus cultivation, and the method comprises the following steps: 1, arranging fungus bags in a shape like a Chinese character'pin ', and carrying out cold stimulation for more than 12 hours at the temperature of below 8 DEG C; 2, cutting the film to expose the material surface, heating at 28 DEG C to return the temperature, refrigerating at 29 DEG C of the core, and controlling the CO2 to be 2000-3000ppm and the humidity to be 80%; 3, after the primordium appears, bud pressing is conducted for 3 hours, the temperature is controlled to be 28 DEG C, the core is 28.5-30 DEG C, and the COO is 3000-5000 ppm; 4, controlling mushrooms for 14.5 hours in a needle tip period, controlling the temperature to be 26-27 DEG C and the COO to be 4000-6000 ppm, and removing deformed mushrooms by combing; 5, cultivating mushrooms in three stages: maintaining and controlling the mushroom parameters for 15 hours in the elongation stage, maintaining and controlling the mushroom parameters for 24-25 DEG C in the young mushroom stage, maintaining and controlling the CO of 5000-7000 ppm for 11.5 hours, maintaining and controlling the CO of 1000-2000 ppm for 4 hours at the temperature of 19 DEG C in the adult mushroom stage; picking mushrooms under the conditions that the temperature is 6.19 DEG C and the COO is 1000-2000ppm; and 7, warehouse cleaning and disinfection. Environmental parameters are accurately controlled in stages, fruiting is uniform, the number of deformed mushrooms is small, the high-quality mushroom rate is high, and the process is standardized and easy to popularize.

This invention discloses a wide-temperature-range *Pleurotus ostreatus* strain and its applications, belonging to the field of edible mushroom breeding and cultivation technology. This *Pleurotus ostreatus* strain has been deposited at CGMCC, with accession number CGMCC No. 42683. This strain requires no low-temperature stimulation or additional CO2 stimulation, and can fruit normally within a wide temperature range of 5℃ to 30℃, exhibiting uniform primordia formation and well-developed fruiting bodies. Its fruiting bodies are grayish-white, light yellowish-brown, or cinnamon brown in color, making them highly recognizable. The cultivation method includes inoculation and mycelial culture, fruiting management, and harvesting. The entire process requires no low-temperature induction, no scratching, and no CO2 supplementation, simplifying management and significantly reducing energy consumption. This strain is early-maturing, high-yielding, and has good continuous cropping ability, with 2-3 harvests per crop. It has a high bioconversion rate, and the fruiting bodies have excellent content of crude protein, crude fat, polysaccharides, and soluble dietary fiber, with a significantly superior taste and flavor compared to existing varieties, possessing extremely high industrial application value.

The present invention relates to water soluble chlorophyll binding protein (WSCP)-chlorophyll complexes as contrast agents for photoacoustic imaging. Disclosed is the use of a WSCP-chlorophyll complex in the preparation of a contrast agent for use in a method of photoacoustic imaging of a subject. Also disclosed are WSCP-chlorophyll complexes comprising Lepidium seu Pleurotus North, WSCP, and chlorophyll a, bacterial chlorophyll a, and bacterial chlorophyll b. The WSCP-chlorophyll complexes of the present invention exhibit strong near infrared (NIR) absorption, unique and non-overlapping spectral characteristics, and concentration dependent photoacoustic (PA) signal generation. These properties make them suitable as contrast agents for PAIs in various applications, particularly in clinical contexts such as disease detection and monitoring.

This invention discloses a plantago rhizosphere strain and its application in the control of rice diseases. The strain is *Pleurotus erythroptera* RSP5, and its classification name is *Pleurotus erythroptera* (…). Talaromyces purpureogenus The strain described in this application, with accession number CGMCC No. 41720, is a purple basket-forming bacterium RSP5 isolated from the rhizosphere soil of Plantago asiatica. This strain not only promotes the growth of rice plants but also exhibits good growth inhibition against rice sheath blight, rice blast, bacterial leaf streak, and bacterial blight. It can be used alone or in combination with pesticides for the control of rice-related diseases.

The invention belongs to the field of microbial biotransformation and degradation technologies and fermentation engineering thereof, and particularly relates to application of pleurotus sajor-caju in biotransformation and degradation. According to the invention, Pleurotus sajor-caju MT is combined with wood pulp sponge to prepare a product capable of rapidly degrading the corpses, the Cu and Cd contents of the corpses and the surrounding environment of the corpses can be reduced in the degradation process, the product has the advantages of ideal degradation time, high public acceptance degree, less peculiar smell and the like, and meanwhile, the surrounding soil and environment can be effectively improved; and benign win-win of surrounding animals and plants is promoted. Particularly, a macro fungus degradation product taking wood pulp sponge as a framework has the price advantage, the production period of the product is shorter, expensive compression molding equipment does not need to be customized, the pollution rate of product culture is reduced, and the application prospect is wide.