60 results about "Flammulina" patented technology

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention relates to the technical field of flammulina velutipes planting, in particular to a flammulina velutipes culture method and compost, which comprises the following steps: S1, preparing purple sweet potato water; the preparation method comprises the following steps: washing fresh purple sweet potatoes, mixing with water in proportion, boiling, cooling, filtering, and taking filtrate as purple sweet potato water for later use; s2, preparing a culture material; the raw materials comprise corncobs, rice bran, bran, cottonseed hulls, sugar beet pulp, soybean hulls, corn flour, brewer's grains, calcium carbonate and shellfish fossils. The preparation method comprises the following steps: weighing solid raw materials in proportion, stirring the raw materials in a stirrer for 10 minutes to obtain a mixture, uniformly mixing the mixture, adding the purple sweet potato water prepared in the step S1, fully stirring the mixture until the mixture is uniformly mixed, and controlling the water content of a final culture material to be 62-68% to obtain the culture medium. The purple sweet potato water is used for completely replacing tap water to serve as a culture material component, the purple sweet potato water is not only a solvent, but also a natural nutrient solution rich in anthocyanin, polysaccharide, vitamins and mineral substances, flammulina velutipes hyphae can absorb and convert the substances, and the anthocyanin content in sporocarp is remarkably increased.

The invention belongs to the technical field of gene editing, and particularly relates to a construction method and application of a needle mushroom CRISPR / Cas9 gene editing vector. The CRISPR / Cas9 gene editing vector for flammulina velutipes is characterized in that the vector takes pFgnpt as a skeleton vector, and further comprises a Cas9 expression cassette and an sgRNA expression cassette; the nucleotide sequence of the Cas9 expression cassette is as shown in SEQ ID NO. 10. The nucleotide sequence of the sgRNA expression cassette is as shown in SEQ ID NO. 11. According to the present invention, with the agrobacterium-mediated transformation technology, the stable expression of Cas9 and sgRNA in the flammulina velutipes body is achieved, the efficient and stable CRISPR / Cas9 gene editing system is established, and the technical support is provided for the flammulina velutipes variety improvement, the functional gene research and the industrial upgrading.

The application discloses a high-sweetness-amino-acid-content Flammulina velutipes variety and a MNP molecular identification method and application thereof. The Flammulina velutipes variety has excellent properties, the cap is not easy to open, the content of sweet amino acids is high, the diversified market demand is met, the factory annual bottle cultivation is suitable, and the Flammulina velutipes variety has a good application and popularization prospect. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' is comprehensive multiple amplification and sequencing technology, sequence analysis of all marker sites of multiple samples can be performed at one time, compared with ISSR, RAPD, SSR and other molecular markers and mushrooming test, the MNP molecular fingerprint has the advantages of high throughput, multi-target, high sensitivity and high precision. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' has specificity and specificity in identifying the Flammulina velutipes strain 'Shangyan A111'.

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The present application provides an automatic picking device for Flammulina velutipes, comprising: a material frame conveying device for conveying the material frame from a growing workshop to a position to be picked; a positioning and clamping device arranged on the conveying device for positioning and clamping the material frame at the position to be picked; a first grabbing assembly comprising a pair of arc-shaped clamping jaws, the arc-shaped clamping jaws being opposite to clamp a cylindrical object and being capable of clamping the Flammulina velutipes and the culture medium apart; a second grabbing assembly comprising oppositely rotatable mechanical fingers, the mechanical fingers being capable of clamping a flaky cylinder to separate the cylindrical mushroom-containing piece from the Flammulina velutipes; and a mushroom-containing piece conveying device for conveying the separated cylindrical mushroom-containing piece to a storage and arrangement part. The device can automatically pick the cylindrical mushroom-containing piece from the bottle opening of the culture bottle and realize automatic picking, weight separation, harvesting and the like of the Flammulina velutipes, optimizes the operation process and helps to improve the growing efficiency.

The invention discloses application of a flammulina velutipes transcription factor gene AreA in regulation and control of cell wall polysaccharide synthesis and sporocarp taste. Flammulina velutipes as an important edible mushroom is widely welcomed due to rich nutrition, but the edible experience of consumers is seriously influenced and the market potential of the flammulina velutipes is limited due to the problems that the flammulina velutipes is poor in chewiness, easy to plug teeth and the like. The silent strain of the enoki mushroom areA gene is successfully constructed through an RNA interference technology, it is found that the polysaccharide content of cell walls and the toughness of sporocarp can be reduced through reduction of gene expression, then the taste of the sporocarp is improved, and the market competitiveness of the sporocarp is improved. According to the invention, the areA gene in the flammulina velutipes is effectively silenced through the specifically designed siRNA fragment, so that the polysaccharide content of the cell wall and the toughness of the sporocarp are reduced, and a theoretical basis is provided for reducing the toughness of the cell wall of the flammulina velutipes by using a genetic engineering technical means.

The invention provides a tablet candy capable of inhibiting fat absorption and a preparation method of the tablet candy, and relates to the technical field of functional foods. The pressed candy capable of inhibiting fat absorption comprises 0.1-25 parts of flammulina velutipes powder, 1-18 parts of L-arabinose, 0.05-5 parts of carob gum, 0.1-12 parts of chestnut powder, 0.1-10 parts of seaweed meal, 0.1-10 parts of instant oolong tea powder, 1-15 parts of composite vegetable powder, 30-80 parts of sorbitol, 3-15 parts of whole milk powder, 0.1-10 parts of edible mushroom concentrated powder, 1-10 parts of yoghurt powder, 1-15 parts of juicy peach juice powder and 0.1-2 parts of citric acid. And 0.1 to 1.5 parts of magnesium stearate. Various functional components such as beta-glucan and L-arabinose in the flammulina velutipes have a synergistic effect, so that the fat adsorption rate is increased; by means of the cold granulation and low-temperature tabletting technology, the retention rate of active ingredients is increased, meanwhile, the product has excellent stability and safety and good taste, the effects of improving intestinal flora and regulating blood sugar can be achieved, and important technical value and wide application prospects are achieved.

The invention discloses a camellia antler SCR0319T2 strain and an application of the camellia antler SCR0319T2 strain. According to the invention, a strain of camellia antler SCR0319T2 is collected and screened from the field, molecular biology identification shows that the strain belongs to Flammulina filiformes, subculture shows that the strain has excellent stability, can still grow stably after more than 30 times of passage, is low in elimination rate, can be used as an industrial large-scale production strain, and is high in strain running speed and short in production period. Through detection, the content of vitamin B1 and nicotinic acid in sporocarps is higher than that of common varieties, and mushroom bodies are durable in storage and good in taste.

This invention discloses an edible aerogel derived from *Flammulina velutipes*, its preparation method, and its applications. The preparation method includes the following steps: dispersing dried *Flammulina velutipes* powder in an aqueous sodium hydroxide solution, reacting, separating, and washing to obtain a precipitate; dispersing the precipitate in an aqueous sodium hypochlorite solution for bleaching, separating, washing, and freeze-drying to obtain an extract; dissolving polyphenols in an aqueous NaOH solution to obtain a NaOH / polyphenol dissolution system; dispersing the extract in the NaOH / polyphenol dissolution system, and shaking to promote CGC swelling; cycling the obtained suspension through freeze-thaw cycles 2-4 times; centrifuging the suspension, and partially dialyzing the solution to remove NaOH to obtain a hydrogel-like CGC-polyphenol complex; freeze-drying and grinding the hydrogel-like CGC-polyphenol complex to obtain an edible aerogel derived from *Flammulina velutipes*. This invention provides the application of the aforementioned edible aerogel derived from *Flammulina velutipes* in the preparation of weight-loss drugs or dietary supplements.

The invention relates to a white flammulina velutipes F523 with the preservation number of GDMCC No.67335. The white flammulina velutipes F523 is high in biological efficiency, short in growth cycle and stable in strain cultivation character, can be stably inherited and is used for flammulina velutipes cultivation and breeding. The invention also relates to an identification method of the flammulina velutipes F523, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether the amplification product has a band with a specific size so as to identify the F523. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

The invention discloses a preparation method of a chitin-glucan hydrogel with a lipid lowering effect and derived from flammulina velutipes. According to the method, firstly, green and environment-friendly enzyme hydrolysis is combined with weak base for treatment, so that the extraction efficiency of the chitin-glucan compound is improved, and the chitin-glucan compound with high acetylation degree, high molecular weight, high crystallinity and good thermal stability is obtained. Secondly, through physical gel preparation of the hydrogel, compared with the hydrogel prepared by the method, the hydrogel prepared by the method has relatively high mechanical property, rheological property and stability, and shows excellent drug loading and releasing capability. The lipid-lowering effect is remarkably enhanced by loading and encapsulating naringenin through the hydrogel and preparing the naringenin into the gel particles, and the huge prospect of the hydrogel in the lipid-lowering aspect is embodied.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention discloses an early-maturing flammulina velutipes variety and application thereof in flammulina velutipes breeding, and belongs to the technical field of flammulina velutipes molecular breeding. According to the invention, a new germplasm is created by using a CRISPR-Cas9 gene editing technology, and the fruiting phenotype of the strain is finally changed by changing a key gene by using the gene editing technology, so that a new variety 'Shangjin No.2' is created. The new variety at least has the following advantages: the yield is high; stipes are long, pilei are internally buckled and thick, and the appearance quality is good; the health-care food has the characteristics of high asparagine content, high glutamine content, high polysaccharide content and high cis-linoleic acid content, and is high in nutritional value; the method has the advantages of short growth period and prematurity. According to the invention, a domestic leading edible mushroom precise breeding technology platform is established, and the method has important strategic significance for improving the core competitiveness of the edible mushroom industry in China.

The invention discloses a method for preparing flammulina velutipes polysaccharide from a deep-eutectic solvent and application of the flammulina velutipes polysaccharide in preservation of volvariella volvacea, and the method comprises the following steps: preparation of the deep-eutectic solvent: mixing ammonium formate and glycerol, heating and stirring to obtain the deep-eutectic solvent; extracting flammulina velutipes polysaccharide: mixing flammulina velutipes residue dry powder with the eutectic solvent, adding water, extracting, performing solid-liquid separation after extraction, and collecting supernate; and precipitating flammulina velutipes polysaccharide: adding ethanol into the supernate for alcohol precipitation, collecting the precipitate, adding a solvent into the precipitate, dialyzing, and freeze-drying. The method lays a foundation for subsequent process optimization and high-value utilization of the flammulina velutipes residues, and provides a theoretical reference for developing a novel and efficient natural-source green extraction solvent. The flammulina velutipes extract disclosed by the invention can effectively delay the senescence process after straw mushroom harvesting through multiple ways of inhibiting water loss, delaying browning, relieving oxidative damage, maintaining tissue morphology and the like, and is a preservative with great application potential.

The utility model discloses a needle mushroom matrix fermentation bed which comprises a bottom plate, a box body is arranged on the bottom plate, a stirring groove is formed in the box body, a motor is arranged on the stirring groove, a group of cams are arranged at the output end of the motor, the output end of the motor is in butt joint with a stirring rod, and the other group of cams are connected with the stirring rod. Before use, raw materials and added substrates are poured into the screen plate on the box body, the scattered raw materials and substrates fall into the stirring tank from holes of the screen plate, the clustered raw materials or substrates remain on the screen plate, and then the motor drives the cam connected to the output end to rotate, so that the raw materials and the added substrates can be uniformly stirred. The cam is in sliding fit with two groups of wheel bodies on the arc-shaped plate, so that the net plate can longitudinally move above the stirring tank to form jolting, the clustered raw materials or substrates are dispersed by vibration and fall into the stirring tank, and then the stirring blades on the stirring rod can be used for stirring more uniformly, so that the stirring efficiency is improved.

The present application relates to the technical field of edible mushroom cultivation, and particularly relates to a flavedo-based flammulina velutipes culture medium and a preparation method thereof, wherein the raw materials comprise, by weight, 15-40 parts of citrus flavedo, 12-25 parts of wood chips, 10-20 parts of bran, 6-8 parts of bean cake powder, 1.5-2.5 parts of gypsum, 1.5-2 parts of sucrose, 0.3-0.8 parts of potassium dihydrogen phosphate, 0.2-0.3 parts of magnesium sulfate, 0.1-0.25 parts of bacillus subtilis and water, and the water is added to make the water content of the mixture reach 62%-67%. The flavedo-based flammulina velutipes culture medium and the preparation method thereof provided by the present application realize efficient resource utilization of fruit and vegetable flavedo waste, reduce the environmental problems caused by biomass waste, replace part of the traditional wood chip raw materials with flavedo, greatly reduce the raw material cost of the culture medium, significantly improve the economic benefits of flammulina velutipes cultivation, and are consistent with the development concept of agricultural circular economy.

The utility model discloses flammulina velutipes packaging equipment which comprises a carrying table, three lower limiting blocks are installed on the upper surface of the carrying table, two first electric push rods are installed on the upper surface of the carrying table, a movable plate is jointly installed at the telescopic ends of the two first electric push rods, three upper limiting blocks are installed on the bottom face of the movable plate, and the upper limiting blocks are installed on the bottom face of the movable plate. And a connecting frame is mounted on the bottom surface of the carrying table. According to the device, three lower limiting blocks and three bagging cylinders are arranged, two first electric push rods are used for driving a moving plate and three upper limiting blocks to move downwards, and meanwhile a second electric push rod is used for pushing a mounting plate, three connecting rods and three push discs to move forwards; according to the flammulina velutipes packaging device, the flammulina velutipes can be clamped, pushed out and packaged in a limited mode, operators do not need to place the flammulina velutipes frequently, the packaging efficiency of the flammulina velutipes is improved, and the flammulina velutipes packaged on the conveying belt can be automatically conveyed and discharged through the arrangement of the conveyor.

The invention relates to a method for extracting ergothioneine from a flammulina velutipes extract, and belongs to the technical field of natural active substance extracting.The method comprises the steps that flammulina velutipes is freeze-dried and smashed, then the smashed flammulina velutipes powder is added into an ethanol water solution for reflux extraction, an extracting solution is obtained, and the extracting solution is concentrated and filtered to obtain the ergothioneine extract. The extracting solution is purified through ultrafiltration, cation exchange resin and anion exchange resin in sequence, then metal salt is added into the collected eluent 2, cooling crystallization is carried out, crystals are collected, an EDTA solution is added for decomplexation, supernate is collected, concentration and drying are carried out, and the high-purity ergothioneine is obtained. Through a series of operations, the purity and the recovery rate of the ergothioneine are remarkably improved.

The invention belongs to the technical field of flammulina velutipes harvesting, and discloses a rapid root cutting device based on flammulina velutipes harvesting, the rapid root cutting device comprises a limiting slide rail support and a sliding plate, a stable clamping assembly is arranged below the sliding plate, the stable clamping assembly comprises a lifting plate, a plurality of limiting frames are mounted at the bottom of the lifting plate, and the limiting frames are arranged on the limiting slide rail support. According to the device, through cooperation of the stable clamping assembly, the lifting plate and other structures, a mechanical structure can be conveniently driven to be adjusted in a pushing mode, the shape of a flexible clamping plate can be changed through adjustment, and the device is convenient to use and high in practicability. The flammulina velutipes can be stably clamped through the deformed arc-shaped flexible clamping plates, and the contact area can be effectively increased through arc-shaped clamping, so that the required clamping force is reduced, and the problem that the flammulina velutipes are damaged due to large force can be effectively avoided while slippage is avoided.

The invention discloses a flammulina velutipes mycelium exopolysaccharide and a preparation method thereof. The flammulina velutipes mycelium exopolysaccharide comprises galactan EPS-N and galactan EPS-A2, the main chain of the galactan EPS-N and the galactan EPS-A2 is composed of alpha-1, 6-connected galactose, the C-2 site of a part of galactose residues is connected with a side chain, and the side chain contains mannose and fucose residues. The preparation method comprises the following steps: (1) providing a liquid fermentation culture of flammulina velutipes mycelia; (2) separating and extracting a flammulina velutipes mycelium exopolysaccharide crude product from the fermentation culture; and (3) separating and purifying the flammulina velutipes mycelium extracellular polysaccharide crude product to obtain the flammulina velutipes mycelium extracellular polysaccharide. The flammulina velutipes polysaccharide can overcome the defects that flammulina velutipes polysaccharide components are complex, active substance structures are not clear enough and the like in the prior art, and solves the problems that wild or solid cultivation flammulina velutipes is limited in resource, unstable in quality, long in production period and the like.

The application discloses a strain of Flammulina velutipes, a fermentation liquor of the strain and application thereof, and belongs to the technical field of microorganisms.The Flammulina velutipes strain R12 is preserved in the Guangdong Provincial Microbial Culture Collection Center on August 18, 2025, the address of the preservation center is No. 59 Building, 5th Floor, Guangzhou Xianlie Middle Road 100, and the preservation number is GDMCC NO. 66855.The fermentation liquor of the Flammulina velutipes strain R12 disclosed by the application has a specific bacteriostatic effect on vancomycin moderately drug-resistant Staphylococcus aureus XN108, and provides an important candidate substance for development of a new type of anti-Staphylococcus aureus drug.

The invention discloses a FeCrNiCo-carbonized flammulina velutipes composite material and a preparation method and application thereof, and the preparation method comprises the following steps: (1) immersing pretreated flammulina velutipes in a mixed metal salt solution containing Fe < 3 + >, Cr < 3 + >, Ni < 2 + > and Co < 2 + >, and loading metal ions on the flammulina velutipes under an oscillation condition; (2) adjusting the pH value of the solution to 8-9, carrying out a co-precipitation reaction to form a metal hydroxide precipitate, and then separating, washing, purifying and drying to obtain a precursor composite material; and (3) carrying out stepped heat treatment on the precursor composite material in a hydrogen-containing protective atmosphere to obtain the FeCrNiCo-carbonized flammulina velutipes composite material. Uniform loading of metal ions is achieved by means of the characteristics of biomass, carbonization and alloying are synchronously completed through multi-stage precise heat treatment, and finally the efficient microwave absorbing material with the characteristics of strong absorption and wide band is obtained.

The invention discloses an early-maturing white flammulina velutipes strain ZJJZG003 with high amino acid content and high brittleness, an InDel marker for identification and application of the strain, and relates to the technical field of edible mushroom breeding, and the preservation number is CGMCC NO.41175. The invention further discloses a preparation method of the strain. The edible mushroom is classified and named as Flammulina filiformis, and the edible mushroom is named as Flammulina filiformis. The strain is obtained by hybridizing F24 and W1 as parents and has a relatively high growth speed, the growth speed of hyphae cultured at 30 DEG C is 5.63 mm / d, the growth speed of hyphae cultured at 25 DEG C is 4.75 mm / d far higher than the growth speed of the parents, and meanwhile, the appearance time of primordium is shortened.

The application discloses a white flammulina velutipes strain ZJJZG003 with early maturity, high amino acid content and high brittleness, an InDel marker for identification and application of the strain, and relates to the technical field of edible mushroom breeding. Flammulina filiformis The strain is obtained by crossing F24 and W1, has a faster growth speed, and has a mycelium growth speed of 5.63 mm / d at 30 DEG C and a growth speed of 4.75 mm / d at 25 DEG C, which are much higher than the growth speeds of the parents, and the time for primordium appearance is shortened.

The application discloses a flammulina velutipes variety X129, an identification method and application thereof. The flammulina velutipes variety X129 is preserved in the China General Microbiological Culture Collection Center (address: No. 3, Beichen West Road, Chaoyang District, Beijing, China) on June 6, 2025, and the biological preservation number is CGMCC No. 42022. The flammulina velutipes variety X129 provided by the application has a thick and non-opening cap, a thick and strong stem, few nodes, small pores in a transverse section and a white cut root.

The invention relates to a yellow flammulina velutipes C53 with a preservation number of GDMCC No.67334. The yellow flammulina velutipes C53 has the advantages of high yield, short growth cycle, more edible parts of stipes, difficulty in browning of stipe bases, long preservation time and stable inheritance, and is used for cultivation and breeding of flammulina velutipes. The invention also relates to an identification method of the flammulina velutipes C53, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether an amplification product has a band with a specific size so as to identify the C53. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

This application discloses a high-resistance, high-brittleness yellow enoki mushroom (ZJJZG002), InDel marker, and their application, relating to the field of edible fungi cultivation technology. The preservation number is CGMCC NO.41174, and the classification name is *Volvariella fuciformis* (also known as 'Silky Cap Winter Mushroom'). Flammulina filiformis The strain ZJJZG002 was obtained by using yellow enoki mushroom F24 and white enoki mushroom W1 as parents. After treating the parental materials with ultraviolet mutagenesis, single spore isolation, hybridization (hybrid combination XB51×YU19), and fruiting tests were carried out. The strain ZJJZG002 does not open its cap easily, has a strong mushroom aroma, a crisp taste, and uniform fruiting. It is suitable for various cultivation methods and can inject new seed sources into the domestic enoki mushroom market.