91 results about "Flammulina" patented technology

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness white flammulina velutipes HJ120 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme of the invention, the high-brittleness white flammulina velutipes HJ120 is obtained, and the preservation number of the flammulina velutipes HJ120 is CCTCC (China Center for Type Culture Collection) NO: M 20242218. The invention also provides a food which comprises the white flammulina velutipes, spores of the white flammulina velutipes or / and an extract of the white flammulina velutipes.

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness yellow flammulina velutipes HJ277 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme, the yellow flammulina velutipes HJ277 with the high brittleness is obtained, and the preservation number of the yellow flammulina velutipes HJ277 is CCTCC (China Center for Type Culture Collection) No: M 20242219. The invention also provides a food which comprises the yellow flammulina velutipes, spores of the yellow flammulina velutipes or / and an extract of the yellow flammulina velutipes.

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention relates to the technical field of flammulina velutipes planting, in particular to a flammulina velutipes culture method and compost, which comprises the following steps: S1, preparing purple sweet potato water; the preparation method comprises the following steps: washing fresh purple sweet potatoes, mixing with water in proportion, boiling, cooling, filtering, and taking filtrate as purple sweet potato water for later use; s2, preparing a culture material; the raw materials comprise corncobs, rice bran, bran, cottonseed hulls, sugar beet pulp, soybean hulls, corn flour, brewer's grains, calcium carbonate and shellfish fossils. The preparation method comprises the following steps: weighing solid raw materials in proportion, stirring the raw materials in a stirrer for 10 minutes to obtain a mixture, uniformly mixing the mixture, adding the purple sweet potato water prepared in the step S1, fully stirring the mixture until the mixture is uniformly mixed, and controlling the water content of a final culture material to be 62-68% to obtain the culture medium. The purple sweet potato water is used for completely replacing tap water to serve as a culture material component, the purple sweet potato water is not only a solvent, but also a natural nutrient solution rich in anthocyanin, polysaccharide, vitamins and mineral substances, flammulina velutipes hyphae can absorb and convert the substances, and the anthocyanin content in sporocarp is remarkably increased.

The invention belongs to the technical field of gene editing, and particularly relates to a construction method and application of a needle mushroom CRISPR / Cas9 gene editing vector. The CRISPR / Cas9 gene editing vector for flammulina velutipes is characterized in that the vector takes pFgnpt as a skeleton vector, and further comprises a Cas9 expression cassette and an sgRNA expression cassette; the nucleotide sequence of the Cas9 expression cassette is as shown in SEQ ID NO. 10. The nucleotide sequence of the sgRNA expression cassette is as shown in SEQ ID NO. 11. According to the present invention, with the agrobacterium-mediated transformation technology, the stable expression of Cas9 and sgRNA in the flammulina velutipes body is achieved, the efficient and stable CRISPR / Cas9 gene editing system is established, and the technical support is provided for the flammulina velutipes variety improvement, the functional gene research and the industrial upgrading.

The present invention belongs to the technical field of extracting effective pharmaceutical ingredients, and particularly relates to a composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and a preparation method and application thereof. The composition comprises a spray-dried powder prepared from citrus fruit filtrate, Flammulina velutipes fiber, soy protein isolate, aronia berry extract, and black tea extract, and a compound probiotic, wherein the compound probiotic comprises Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis, and Lactobacillus casei. The present invention adopts compound spraying of Flammulina velutipes fiber, soy protein isolate, aronia berry extract, black tea extract, and citrus fruit filtrate to improve the stability of total polyphenols and Vc in citrus fruits. The obtained citrus powder is further mixed with a compound probiotic to prepare a composition with pharmacologically active ingredients, which can effectively burn fat and lose weight and can be applied to weight loss drugs.

The invention relates to the technical field of flammulina velutipes cultivation, in particular to a flammulina velutipes strain culture environment precise regulation and control device and an intelligent control method thereof.The flammulina velutipes strain culture environment precise regulation and control device comprises a flammulina velutipes strain culture box body, an air inlet pipe is fixedly connected to the outer wall of the top of the flammulina velutipes strain culture box body in a penetrating mode, and a mounting shell is fixedly connected to the outer wall of the bottom of the air inlet pipe; a controller is fixedly connected to the outer wall of one side of the flammulina velutipes strain incubator body, a motor is fixedly connected to the outer wall of one side of the flammulina velutipes strain incubator body, and a water tank is fixedly connected to the outer wall of the other side of the flammulina velutipes strain incubator body. The heating plate is driven by the motor to adjust the angle, the spreading direction of heat can be changed, the heat can cover all horizontal areas of the needle mushroom strain incubator body more evenly, it is guaranteed that all needle mushroom strains are under the similar temperature condition, the needle mushroom strains grow evenly, and workers can freely adjust the angle of the heating plate according to site requirements.

The application discloses a high-sweetness-amino-acid-content Flammulina velutipes variety and a MNP molecular identification method and application thereof. The Flammulina velutipes variety has excellent properties, the cap is not easy to open, the content of sweet amino acids is high, the diversified market demand is met, the factory annual bottle cultivation is suitable, and the Flammulina velutipes variety has a good application and popularization prospect. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' is comprehensive multiple amplification and sequencing technology, sequence analysis of all marker sites of multiple samples can be performed at one time, compared with ISSR, RAPD, SSR and other molecular markers and mushrooming test, the MNP molecular fingerprint has the advantages of high throughput, multi-target, high sensitivity and high precision. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' has specificity and specificity in identifying the Flammulina velutipes strain 'Shangyan A111'.

The utility model relates to the technical field of flammulina velutipes liquid processing, and discloses a feeding structure of a flammulina velutipes liquid enlarged culture kettle, which comprises a blanking barrel, a guide barrel is rotatably arranged in the blanking barrel, the size of the top of the guide barrel is larger than that of the bottom of the guide barrel, one side of the guide barrel is inclined, and the other side of the guide barrel is inclined. A barrel cover is arranged at the top end of the discharging barrel, a feeding hopper is rotationally arranged in the middle of the barrel cover, a material blocking disc is embedded in the bottom end of the discharging barrel and connected with the bottom of the discharging barrel through fixing screws, and a plurality of annularly-distributed discharging pipes are arranged at the bottom end of the discharging barrel and correspond to the discharging end of the guiding barrel. The position of the discharging end of the material guide barrel can be adjusted through the material guide barrel which is rotationally arranged, and a plurality of discharging pipes corresponding to the position of the discharging end of the material guide barrel are arranged at the bottom, so that materials can be discharged into different positions in the kettle body, the accumulation of the materials is reduced, and the mixing effect of subsequent materials is improved.

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The present application provides an automatic picking device for Flammulina velutipes, comprising: a material frame conveying device for conveying the material frame from a growing workshop to a position to be picked; a positioning and clamping device arranged on the conveying device for positioning and clamping the material frame at the position to be picked; a first grabbing assembly comprising a pair of arc-shaped clamping jaws, the arc-shaped clamping jaws being opposite to clamp a cylindrical object and being capable of clamping the Flammulina velutipes and the culture medium apart; a second grabbing assembly comprising oppositely rotatable mechanical fingers, the mechanical fingers being capable of clamping a flaky cylinder to separate the cylindrical mushroom-containing piece from the Flammulina velutipes; and a mushroom-containing piece conveying device for conveying the separated cylindrical mushroom-containing piece to a storage and arrangement part. The device can automatically pick the cylindrical mushroom-containing piece from the bottle opening of the culture bottle and realize automatic picking, weight separation, harvesting and the like of the Flammulina velutipes, optimizes the operation process and helps to improve the growing efficiency.

The present invention belongs to the field of composite materials technology, specifically relating to an edible mycorrhizal glycoconjugate-graphene conductive film and its preparation method. This invention uses chitosan-glucan complex (CGC), a root glycoconjugate extracted from the mycorrhizae of Flammulina velutipes, as raw material. Extracting CGC from the mycorrhizae requires only mild acid and alkali treatment to remove components such as proteins, minerals, and lipids, while also minimizing damage to the CGC and improving its purity. The prepared CGC, when used as a dispersant, improves graphene agglomeration. Compared to graphene without CGC, at the optimal concentration of CGC added, the median particle size decreased by 43.11%, and the absolute value of the zeta potential increased by 160.07%. The addition of CGC to graphene significantly enhances its dispersibility, improving the tensile strength and conductivity of the CGC-graphene film.

The invention discloses application of a flammulina velutipes transcription factor gene AreA in regulation and control of cell wall polysaccharide synthesis and sporocarp taste. Flammulina velutipes as an important edible mushroom is widely welcomed due to rich nutrition, but the edible experience of consumers is seriously influenced and the market potential of the flammulina velutipes is limited due to the problems that the flammulina velutipes is poor in chewiness, easy to plug teeth and the like. The silent strain of the enoki mushroom areA gene is successfully constructed through an RNA interference technology, it is found that the polysaccharide content of cell walls and the toughness of sporocarp can be reduced through reduction of gene expression, then the taste of the sporocarp is improved, and the market competitiveness of the sporocarp is improved. According to the invention, the areA gene in the flammulina velutipes is effectively silenced through the specifically designed siRNA fragment, so that the polysaccharide content of the cell wall and the toughness of the sporocarp are reduced, and a theoretical basis is provided for reducing the toughness of the cell wall of the flammulina velutipes by using a genetic engineering technical means.

The invention provides a tablet candy capable of inhibiting fat absorption and a preparation method of the tablet candy, and relates to the technical field of functional foods. The pressed candy capable of inhibiting fat absorption comprises 0.1-25 parts of flammulina velutipes powder, 1-18 parts of L-arabinose, 0.05-5 parts of carob gum, 0.1-12 parts of chestnut powder, 0.1-10 parts of seaweed meal, 0.1-10 parts of instant oolong tea powder, 1-15 parts of composite vegetable powder, 30-80 parts of sorbitol, 3-15 parts of whole milk powder, 0.1-10 parts of edible mushroom concentrated powder, 1-10 parts of yoghurt powder, 1-15 parts of juicy peach juice powder and 0.1-2 parts of citric acid. And 0.1 to 1.5 parts of magnesium stearate. Various functional components such as beta-glucan and L-arabinose in the flammulina velutipes have a synergistic effect, so that the fat adsorption rate is increased; by means of the cold granulation and low-temperature tabletting technology, the retention rate of active ingredients is increased, meanwhile, the product has excellent stability and safety and good taste, the effects of improving intestinal flora and regulating blood sugar can be achieved, and important technical value and wide application prospects are achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to flammulina velutipes foot polysaccharide with low molecular weight and application thereof. The flammulina velutipes foot waste is used as a raw material, after steam explosion and enzymolysis treatment, ethanol with different volume fractions is adopted for gradient separation, the flammulina velutipes foot polysaccharide with the specific molecular weight is obtained, and after test verification, the obtained flammulina velutipes foot polysaccharide has a good inflammation inhibition effect, and the flammulina velutipes foot polysaccharide can be used for preparing the flammulina velutipes foot polysaccharide. Therefore, the compound can be developed and applied as a natural anti-inflammatory agent.

The invention discloses an application of a flammulina velutipes extract in preparation of related products for relieving and / or treating drug-induced liver injury. By constructing a drug-induced liver injury model, it is found that by means of the needle mushroom extract rich in needle mushroom polysaccharide in low dosage, liver pathological injuries such as cisplatin-induced hepatocyte swelling and necrosis can be remarkably relieved, the levels of serum liver function indexes of alanine transaminase and aspartate transaminase are reduced, and the liver injury resistance is improved. And meanwhile, the activity of superoxide dismutase and glutathione peroxidase in liver tissues is improved, the concentration of malonaldehyde is reduced, and excellent antioxidant characteristics are shown. In addition, the invention also discovers that the flammulina velutipes extract can improve the composition of intestinal microorganisms, promote colonization of beneficial bacteria, reduce the abundance of harmful bacteria and regulate the key metabolic pathway of the liver at the same time, and can provide a treatment strategy for the treatment of drug-induced liver injury.

The invention discloses a camellia antler SCR0319T2 strain and an application of the camellia antler SCR0319T2 strain. According to the invention, a strain of camellia antler SCR0319T2 is collected and screened from the field, molecular biology identification shows that the strain belongs to Flammulina filiformes, subculture shows that the strain has excellent stability, can still grow stably after more than 30 times of passage, is low in elimination rate, can be used as an industrial large-scale production strain, and is high in strain running speed and short in production period. Through detection, the content of vitamin B1 and nicotinic acid in sporocarps is higher than that of common varieties, and mushroom bodies are durable in storage and good in taste.

The invention relates to the technical field of health care product preparation, in particular to fat dissolving powder and a preparation method thereof. Comprising the following raw materials in parts by weight: white kidney beans, flammulina velutipes, resistant dextrin, food essence, lactitol, inulin, vegetable and fruit plant enzyme powder, apple powder, compound probiotic powder, edible glucose, sorbitol, polydextrose, fructo-oligosaccharide, stachyose, xylooligosaccharide, L-arabinose, lactose and galactooligosaccharide. The fat dissolving powder disclosed by the invention selects the white kidney beans and the needle mushrooms as main materials, has the effects of promoting intestinal tract movement, accelerating the detoxification speed, helping a body to burn redundant fat, losing weight and beautifying, is rich in antioxidant substances, such as vitamin C and polyphenol compounds, and the components are beneficial to scavenging free radicals and reducing oxidative stress, so that the aging process is delayed, and the fat dissolving powder is suitable for being eaten by people. Various saccharides are added to further improve the intestinal environment, and L-arabinose is supplemented to inhibit the absorption of cane sugar and disaccharide in high-sugar and high-fat diet so as to achieve the effect of reducing blood sugar.

The invention belongs to the technical field of edible mushroom genetic engineering, and relates to a method for constructing a mushroom genetic transformation system by using a G418 resistance selection marker, which comprises the following steps: taking a mushroom strain Shenxiang 215 cultured in a PDA (potato dextrose agar) culture medium as a receptor to obtain hypha blocks; the method comprises the following steps: transferring a pLgnpt plasmid into agrobacterium tumefaciens GV3101; agrobacterium tumefaciens is inoculated into an IM liquid culture medium containing acetosyringone and MES, hypha blocks are subjected to ultrasonic treatment and co-incubation with the hypha blocks, then the hypha blocks are transferred to an IM solid culture medium laid with filter paper, and dark culture is carried out; and transferring the hyphae subjected to dark culture to a PDA resistant plate culture medium containing G418 and Cef for resistance screening to obtain the shiitake transformant. The method not only verifies the applicability of the novel selection marker in flammulina velutipes, but also constructs a multi-marker technology platform parallel to the existing hygromycin system, and lays a foundation for subsequent complex operations such as multi-gene superposition editing, gene loop construction and the like.

This invention discloses an edible aerogel derived from *Flammulina velutipes*, its preparation method, and its applications. The preparation method includes the following steps: dispersing dried *Flammulina velutipes* powder in an aqueous sodium hydroxide solution, reacting, separating, and washing to obtain a precipitate; dispersing the precipitate in an aqueous sodium hypochlorite solution for bleaching, separating, washing, and freeze-drying to obtain an extract; dissolving polyphenols in an aqueous NaOH solution to obtain a NaOH / polyphenol dissolution system; dispersing the extract in the NaOH / polyphenol dissolution system, and shaking to promote CGC swelling; cycling the obtained suspension through freeze-thaw cycles 2-4 times; centrifuging the suspension, and partially dialyzing the solution to remove NaOH to obtain a hydrogel-like CGC-polyphenol complex; freeze-drying and grinding the hydrogel-like CGC-polyphenol complex to obtain an edible aerogel derived from *Flammulina velutipes*. This invention provides the application of the aforementioned edible aerogel derived from *Flammulina velutipes* in the preparation of weight-loss drugs or dietary supplements.

The invention relates to a white flammulina velutipes F523 with the preservation number of GDMCC No.67335. The white flammulina velutipes F523 is high in biological efficiency, short in growth cycle and stable in strain cultivation character, can be stably inherited and is used for flammulina velutipes cultivation and breeding. The invention also relates to an identification method of the flammulina velutipes F523, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether the amplification product has a band with a specific size so as to identify the F523. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

The invention discloses a preparation method of a chitin-glucan hydrogel with a lipid lowering effect and derived from flammulina velutipes. According to the method, firstly, green and environment-friendly enzyme hydrolysis is combined with weak base for treatment, so that the extraction efficiency of the chitin-glucan compound is improved, and the chitin-glucan compound with high acetylation degree, high molecular weight, high crystallinity and good thermal stability is obtained. Secondly, through physical gel preparation of the hydrogel, compared with the hydrogel prepared by the method, the hydrogel prepared by the method has relatively high mechanical property, rheological property and stability, and shows excellent drug loading and releasing capability. The lipid-lowering effect is remarkably enhanced by loading and encapsulating naringenin through the hydrogel and preparing the naringenin into the gel particles, and the huge prospect of the hydrogel in the lipid-lowering aspect is embodied.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention discloses an early-maturing flammulina velutipes variety and application thereof in flammulina velutipes breeding, and belongs to the technical field of flammulina velutipes molecular breeding. According to the invention, a new germplasm is created by using a CRISPR-Cas9 gene editing technology, and the fruiting phenotype of the strain is finally changed by changing a key gene by using the gene editing technology, so that a new variety 'Shangjin No.2' is created. The new variety at least has the following advantages: the yield is high; stipes are long, pilei are internally buckled and thick, and the appearance quality is good; the health-care food has the characteristics of high asparagine content, high glutamine content, high polysaccharide content and high cis-linoleic acid content, and is high in nutritional value; the method has the advantages of short growth period and prematurity. According to the invention, a domestic leading edible mushroom precise breeding technology platform is established, and the method has important strategic significance for improving the core competitiveness of the edible mushroom industry in China.

The invention discloses a method for preparing flammulina velutipes polysaccharide from a deep-eutectic solvent and application of the flammulina velutipes polysaccharide in preservation of volvariella volvacea, and the method comprises the following steps: preparation of the deep-eutectic solvent: mixing ammonium formate and glycerol, heating and stirring to obtain the deep-eutectic solvent; extracting flammulina velutipes polysaccharide: mixing flammulina velutipes residue dry powder with the eutectic solvent, adding water, extracting, performing solid-liquid separation after extraction, and collecting supernate; and precipitating flammulina velutipes polysaccharide: adding ethanol into the supernate for alcohol precipitation, collecting the precipitate, adding a solvent into the precipitate, dialyzing, and freeze-drying. The method lays a foundation for subsequent process optimization and high-value utilization of the flammulina velutipes residues, and provides a theoretical reference for developing a novel and efficient natural-source green extraction solvent. The flammulina velutipes extract disclosed by the invention can effectively delay the senescence process after straw mushroom harvesting through multiple ways of inhibiting water loss, delaying browning, relieving oxidative damage, maintaining tissue morphology and the like, and is a preservative with great application potential.

The utility model discloses a needle mushroom matrix fermentation bed which comprises a bottom plate, a box body is arranged on the bottom plate, a stirring groove is formed in the box body, a motor is arranged on the stirring groove, a group of cams are arranged at the output end of the motor, the output end of the motor is in butt joint with a stirring rod, and the other group of cams are connected with the stirring rod. Before use, raw materials and added substrates are poured into the screen plate on the box body, the scattered raw materials and substrates fall into the stirring tank from holes of the screen plate, the clustered raw materials or substrates remain on the screen plate, and then the motor drives the cam connected to the output end to rotate, so that the raw materials and the added substrates can be uniformly stirred. The cam is in sliding fit with two groups of wheel bodies on the arc-shaped plate, so that the net plate can longitudinally move above the stirring tank to form jolting, the clustered raw materials or substrates are dispersed by vibration and fall into the stirring tank, and then the stirring blades on the stirring rod can be used for stirring more uniformly, so that the stirring efficiency is improved.

The invention provides unsaturated fatty acid in flammulina velutipes mononuclear mycelium. The unsaturated fatty acid compound is one or more of structural formulas 1 and 2. Unsaturated fatty acid in flammulina velutipes mononuclear mycelium provided by the invention has an anti-inflammatory effect, and has a wide application prospect, # imgabs0 #

The invention discloses an immunomodulatory polypeptide and application thereof. The invention discovers that a peptide fragment KR5 obtained by enzymolysis of flammulina velutipes immunomodulatory protein FIP-fve and a part of homologous peptide fragments from different fungus sources both have better immunomodulatory characteristics, and the amino acid sequence of the peptide fragment KR5 is B-Y-J-YR. After the KR5 is subjected to reversed-sequence overturning and modification transformation, the immune activation effect of the obtained D-configuration polypeptide and modified polypeptide is unchanged compared with that of the KR5, and both the D-configuration polypeptide and the modified polypeptide are superior to that of the flammulina velutipes immunomodulatory protein FIP-fve. In a mouse CT26 colorectal cancer model, the rk5 can significantly inhibit tumor growth, and the therapeutic effect is equivalent to that of a PD-L1 antibody. The FIP active polypeptide provided by the invention provides a theoretical basis for exploring pharmacodynamic substances and action mechanisms of FIP, provides candidate drugs for tumor immunologic diagnosis and treatment, and has a good application prospect and a relatively high economic value.