872 results about "Flammulina" patented technology

A method for cultivating golden mushroom mycelia abundant in Se and Zn elements through liquid fermentation belongs to the field of biotechnology. The invention substantially comprises cultivation of strains, cultivation of liquid seed, namely the domestication of two elements, and enrichment, fermentation and cultivation of two elements in a deep liquid, and collecting, drying and smashing of mycelia. The dried golden mushroom mycelia abundant in organic Se and organic Zn obtained with the method of the invention has over 35Mug/g organic Se and 4.25mg/g organic Zn. The powder of the mycelia with suitable food can be used as food additives and made into functional food, which has great health care function for the people in sub-health state resulted from deficient intake of microelements of Se and Zn. The invention is high in technological content, high content in effective components of the products, high additive value, which is suitable for industrial production.

The present invention provides a formula of high-yield flammulina velutipes culture medium and a producing technique thereof. The invention relates to the technical filed of biological strain, and specially to the formula of high-yield flammulina velutipes culture medium and producing technique thereof. The corncob is used as main ingredient for replacing fir sawdust. The invention adopts 40-45% of corncob, 10-12% of bagasse, 25-30% of rice bran, 8-10% of bran, 4-6% of soybean hull and 2-3% of corn flour as the raw materials of flammulina velutipes culture medium. The method comprises the techniques of dry mixing, wet mixing, etc. The pH value is controlled between 7.0 and 7.5. The cultured flammulina velutipes has the advantages of strong activity of mycelium, high production volume, ordered mushroom generation, excellent commodity valve, ordered growing of sporphore, uniform magnitude of bonnet, white color, and no infection of foreign fungus. The unit production obtains 286-293grams/bottle. The dry object of each bottle is 260grams. The biological transformation efficiency reaches up to 110-113% and is increased for 30-40% compared with the common formula. The economic benefit is excellent.

The invention relates to new technology and a new method suitable for the storage and fresh keeping of edible mushrooms, belongs to the technical fields of the storage and processing of agricultural products and agricultural horticulture in food science and engineering, and relates to comprehensive technology and a comprehensive method for high-oxygen atmosphere packaging of the edible mushrooms, which can be applied to the storage and fresh keeping of flammulina velutipes, button mushrooms, pleurotus ferulae, lentinus edodes and the like. The edible mushrooms are delicious in taste and rich in nutrients, and have the diet therapy and health care effects of reducing cholesterol, preventing hypertension, gastrointestinal ulcer, hepatopathy and hyperlipemia, resisting cancers, improving organism immunity and the like. The edible mushrooms such as flammulina velutipes, the button mushrooms and the like are fresh food predominantly, but fresh mushrooms are easy to generate brown stain, pileus stretching and decay after picking due to high moisture, and thus the fresh mushrooms are short in refreshing time. The invention adopts the comprehensive fresh-keeping technology and the comprehensive fresh-keeping method such as high-oxygen atmosphere packaging technology (HOAP), natural food preservatives, food absorbent and the like to achieve the aims of retaining the freshness of the edible mushrooms, restraining bacteria, avoiding moisture loss, preventing pileus from stretching and browning, preventing the edible mushrooms from generating peculiar smell and prolonging the storage refreshing time of the edible mushrooms.

The invention belongs to the technical field of edible fungi cultivation, and discloses an industrialized production method for flammulina velutipes in bags. Only corncobs, cottonseed hulls, bran and corn flour are selected as main raw materials, the industrialized production method comprises the steps of detection and acceptance inspection for the raw materials and auxiliary materials, dosing, pre-wetting, mixing, bagging, sterilization, cooling, inoculation, cultivation scratching, breeding, harvesting, packaging and the like, and industrialized production condition control is carried out. The industrialized production method is simple in incoming material and low in cost, the produced flammulina velutipes have the advantages of being high in nutritive value, short in production cycle, low in production cost and the like, labor efficiency and cultivation efficiency are improved, and comprehensive economic benefits are high.

The invention discloses a flammulina velutipes culture medium and a preparation method thereof. The flammulina velutipes culture medium comprises the following components by mass percent: 30-54 percent of traditional Chinese medicine residue, 10-25 percent of cottonseed hull, 15-25 percent of wheat bran, 20-30 percent of corncob and 0.5-1.5 percent of light calcium carbonate. According to the technical scheme provided by the invention, raw material sources of the flammulina velutipes culture medium are increased, so that a positive role is played on stabilizing cottonseed hull; during production of the flammulina velutipes culture medium, the traditional Chinese medicine residues are directly crushed and compounded without being dried or aired, so that the production cost of flammulina velutipes is greatly lowered; and when the culture medium is used for culturing flammulina velutipes, compared with a cottonseed hull medium, the manufacturing cost is lowered and the flammulina velutipes quality is greatly improved.

The invention relates to composite barley green solid beverage and a production process thereof and belongs to the technical field of deep processing of agricultural products. The invention is characterized in that: the composite barley green solid beverage is prepared by using fresh barley grass juice and barley green powder as main raw materials and adding barley grass dreg powder, spiral seaweed powder, xanthan gum, sorbitol, sodium alginate, maltodextrin and sodium carboxymethylcellulose through procedures of sterilizing and packaging, wherein the fresh barley grass juice is used for performing biological green protection by using flammulina velutipes extracting solution; and the barley green powder is prepared through vacuum freeze drying. The composite barley green solid beverage have the characteristics of simple production process, high utilization rate of the raw materials and the like, can be produced industrially, has high nutritional value and high drinking performance and is ideal natural health-care beverage.

The invention discloses a flammulina velitupe residue culture medium material and a method for culturing agaricus bisporus. The flammulina velitupe residue culture medium material comprises the following active ingredients in parts by weight: 30-60 parts of flammulina velitupe residues, 35 parts of cow dung, 0.5-1 part of bagasse residues, 0.5-1.5 parts of silkworm faeces, 1 part of compound fertilizer, 1-1.5 parts of lime, 0.5-1 part of gypsum, and 2 parts of calcium superphosphate. The method for culturing agaricus bisporus comprises the steps: piling up, fermenting, sowing, earthing, fruiting and recovering to obtain agaricus bisporus. The fruiting period of the agaricus bisporus is advanced by seven to ten days, the agaricus bisporus recovery rate is prolonged by 10-15 days, the flammulina velitupe residues can be recycled, and the pollution of the flammulina velitupe residue on the environment can be reduced.

The invention discloses a method for preparing flammulina velutipes polysaccharides and protein efficiently synchronously. The method comprises the following steps of: grinding flammulina velutipes sporocarps, pretreating by using a cellulose, extracting by microwave, centrifuging and precipitating in alcohol to obtain the crude flammulina velutipes polysaccharides; dissolving the crude flammulina velutipes polysaccharides into water, adding an ionicliquid/salt aqueous two-phase system, mixing uniformly and centrifuging to obtain an upper phase and a lower phase; taking out the lower layer which is rich in the crude flammulina velutipes polysaccharides, removing salt by using an ultrafiltration membrane with the retention capacity of 6,000, concentrating, freezing and drying to obtain the flammulina velutipes polysaccharides; adding methylene dichloride into the upper layer which is rich in proteins, extracting, drying rotationally and removing the methylene dichloride to obtain the flammulina velutipes protein. The method can be used for preparing the flammulina velutipes polysaccharides and protein synchronously, can improve the extraction rate of the polysaccharides and the protein, is simple in process and low in equipment requirement and is suitable for large-scale production.

The invention relates to a white flammulina velutipes culture medium formula, which consists of the following raw materials in percent by weight: 9-11 percent of cotton seed hull, 12-14 percent of sawdust, 30.5-32.5 percent of wheat bran, 24-26 percent of corn cob, 12-14 percent of rice bran, 3-5 percent of corn, 1-3 percent of soybean meal and 0.5-2.5 percent of calcium carbonate. The white flammulina velutipes culture medium formula has the advantages that the formula is reasonable, the yield and the quality of white Flammulina velutipes can be greatly improved and the economic benefits are increased.

The invention relates to a method for preparing flammulina velutipes polysaccharide with simple operation and low equipment investment, which comprises the following process procedures: activating a slant strain, shaking culture of seeds, submerged fermentation in liquid, continuous fermentation, separation by an ultrafiltration membrane, condensation and alcohol precipitation. The method for preparing the flammulina velutipes polysaccharide has the advantages of continuous fermentation in a fermenter, prolonging the fermentation time, simplifying the operation flow and improving the polysaccharide yield.

The invention discloses a fermented composition of multiple probiotics and composite medicinal and edible fungi, and a preparation method and an application thereof. According to the invention, a composite polysaccharide extract is obtained by enzymolysis and extraction of various medicinal and edible fungi by using compound enzymes, and the extract is subjected to symbiotic fermentation by using multiple intestinal probiotics, such that a fermented composition is obtained. The invention also relates to an application of the fermented composition in preparing health-care products used for preventing and treating tumors. The medicinal and edible fungi adopted by the invention are any three or more than three selected from lucid ganoderma, agaricus blazei murill, shiitake, and flammulina velutipes. The fungi are subjected to crushing, soaking, and enzymolysis; and bifidobacterium and lactobacillus are added for fermentation. Through symbiotic fermentation with the intestinal probiotics, the molecular weight of medicinal and edible fungi composite polysaccharide is substantially reduced, tumor cell inhibiting activity is substantially improved, and inhibition effect is substantially improved.

The invention discloses a method for cultivating tomatoes in a greenhouse in a soilless mode. The method comprises the steps of designing cultivating grooves, preparing a substrate, sterilizing the substrate, preparing and applying a nutrient solution, and conducting comprehensive tomato cultivation management. A half of each cultivating groove is buried into soil, so that a great number of materials is saved. The substrate is divided into two layers. All the underground substrate is furnace slag. The substrate used on the ground is flammulina velutipese residues. The substrate has a large porosity, so that respiration, extension, water absorption and nutrient absorption of root systems are facilitated. The substrate is sterilized at the temperature higher than 70 DEG C, and the main target is the tomato mosaic disease. When tomatoes are cultivated according to the method for cultivating tomatoes in the greenhouse in the soilless mode, the use amount of pesticide and fertilizer and labor cost are reduced, and the tomato yield, the content of carbohydrate and the content of Vitamin C are all greatly increased. According to the method for cultivating tomatoes in the greenhouse in the soilless mode, the practicability is high, and high economic value is achieved.

The invention discloses a culture method of flammulina velutipes liquid strains, which comprises the steps of the culture medium preparation, sterilization, inoculated culture and the like. A culture medium comprises soybean powder, white granulated sugar, magnesium sulfate and monopotassium phosphate; and after the liquid culture medium is added into a liquid strain culture tank with an external heat source, the culture tank is pushed into a sterilizing pot for sterilization, the culture tank is cooled to room temperature for inoculation after the sterilization ends, and then sterile compressed air is introduced into the culture tank for culture. The culture medium of the method has the advantages of reasonable formula and rich nutrients; the liquid culture medium in the liquid strain culture tank adopting the external heat source is heated uniformly without damaging nutrient components. The liquid strains cultured by the method have the advantages of short production cycle, easy quality control, high spawn running speed, tidy fruiting, and high yield.

The invention discloses a culture medium for edible fungi. The prepared culture medium for edible fungi is prepared from the following raw materials in percentage by mass: 65.34-81.54% of salix mongolica wood flour, 4.02-19.89% of bran, 3.82-10.49% of bean pulp, 3.72-7.15% of corn flour and 2.0% of plaster stone, wherein the sum of the mass percents of the raw materials is 100%. The culture medium for edible fungi is suitable for cultivation of multiple edible fungi such as Pleurotus eryngii, Flammulina velutipes, Pleurotus citrinopileatus, Pleurotus ostreatus, Pleurotus nebrodensis, Lentinus edodes, Exidia auricuia and the like.

The method of cultivating edible fungi by using the discarded branches and leaves of urban greening, at least one kind of urban greening branches and leaves among ginkgo, zelkova, magnolia or white birch is preliminarily sorted, washed, dried, crushed, mixed with nutrients and water, and stirred evenly , to make edible fungus culture material, put the edible fungus culture material into bags and carry out high-temperature sterilization. Flammulina velutipes common edible fungus. The invention uses the discarded branches and leaves of landscaping as raw materials, which not only effectively solves the problem of shortage of raw materials for cultivating edible fungi, but also reasonably solves the problem of waste disposal in current landscaping. The edible fungi cultivated by the invention are rich in nutrition, good in mushroom quality and high in yield ,with broadly application foreground.

The invention relates to a device and method of intelligent light supplementation of flammulina velutipes in different growth periods based on a convolutional neural network. The device of light supplementation is composed of a bearing device, a camera (the revolution is 1920*1080), a full spectrum LED lamp, a computer, a flammulina velutipes culture medium and a plastic pipe. The method comprises the steps of using image samples of massive manually labeled flammulina velutipes in the different growth periodss to train the convolutional neural network to obtain a trained classification model; using the camera to shoot growing flammulina velutipes in a planting room to conduct sampling, and using the trained model to judge which growth periods the flammulina velutipes samples belong to; then using the computer to control the light color of the full spectrum LED lamp according to the requirement of flammulina velutipes for light quality in the growth period, so that the light color of the full spectrum LED lamp meets the requirement most suitable for light quality of flammulina velutipes in the current growth period, and finally a promotion effect is played on the growth of flammulina velutipes by controlling the light quality to achieve the production aim of planting personnel. According to the device and method of the intelligent light supplementation of flammulina velutipes in the different growth periods based on the convolutional neural network, the yield of flammulina velutipes is increased, the quality of flammulina velutipes is improved, so that producers make a profit, and consumers are benefited.

The invention discloses a flammulina velutipes culture medium. The flammulina velutipes culture medium comprises raw materials in parts by weight as follows: 50-60 parts of corncob, 9-19 parts of bran, 5-8 parts of rice bran, 5-15 parts of oilseed rape meal, 5-15 parts of soybean hull, 1-2 parts of gypsum powder, 1-2 parts of shell fossil and 1-2 parts of peppermint oil. A preparation method of the flammulina velutipes culture medium comprises preparation of raw materials, bottling, sterilization and cooling, wherein preparation of raw materials comprises preparation of a dry material and preparation of a wet material; preparation of the wet material is as follows: a part of materials are mixed to sufficiently suck water, citric acid or pyruvic acid are added to adjust the pH value after alcoholic fermentation, then gypsum powder, shell fossil and a nutrition liquid are added, the mixture is treated on a shaking table at a constant temperature of 25 DEG C for 10-20 minutes, and the mixture is rotationally distillated for 1-2 hours till the water content is 50%-60%; and the cooled culture medium is used for inoculating flammulina velutipes. The flammulina velutipes culture medium is rich in iron, the use amount of rice bran and the cost are reduced, the water content in the culture medium is appropriate, and nutrition components are easy to absorb by flammulina velutipes.

The invention discloses a storing and fresh preserving method for white flammulina velutipes, which pertains to the technical field of storage and fresh preservation of agricultural commodity. The method comprises the steps of: (1) harvesting, finishing and grading of the white flammulina velutipes; (2) preparation of anti-browning inhibitors and the treatment thereof; (3) treatment of low temperature and dryness; (4) vacuum packaging; (5) storage at ice temperature until delivering out of a storehouse and distributing to the market. The white flammulina velutipes stored by the method is characterized by reducing the rotting rate, enzymatic browning and the respiration rate, lengthening the preservation period which reaches 20 to 30 days, keeping the original color, fragrance, taste, shape and nutrition, etc., and reaching a commodity rate over 95 percent. The anti-browning inhibitors are the food additives which are permitted by the country and are safe and reliable. The method of the invention can be widely applied in the fields of production, storage and fresh preservation and operation of the flammulina velutipes.

The invention relates to a SSR label fingerprint of a flammulina velutipes F3 strain and applications thereof. The fingerprint is composed of six pairs of SSR labeled specific allelic sections which are developed on the basis of the genomic sequence of flammulina velutipes. The fingerprint can be used to identify the flammulina velutipes F3 strain, and the identification comprises the following steps: carrying out SSR labeling amplification on a flammulina velutipes strain, and then comparing the obtained fingerprint with the fingerprint of flammulina velutipes F3 strain, if the two fingerprints are identical, the flammulina velutipes strain is the F3 strain. Compared to the conventional morphology detection, antagonism tests, and fruiting experiments, the fingerprint identification method has the advantages of short detection time, high accuracy, and good repeatability, and has the specificity on F3 strain in collected 24 flammulina velutipes strains which are commonly cultured in China.

The invention discloses an edible mushroom parent strain culture medium and a preparation method thereof. The culture medium comprises the following components: 200g of peeled potatoes, 40g of hard wood chips, 10g of glucose, 10g of cane sugar, 2g of monopotassium phosphate, 1.5g of magnesium sulfate, 10-15mg of gibberellin, 10-15mg of 2.4-D, 18-20g of agar and 1,000ml of water. The culture medium is based on study on biological characteristics of different edible mushroom strains, scientific methods are used, a proper amount of plant growth regulating agents are added into the culture medium, on premise that the quality of the edible mushroom strain is not affected, a novel edible mushroom variety of an edible mushroom variety breeding person is prevented from expanding propagation by others, and the intellectual property of the strain breeding person is not abused by others. The culture medium is applicable to parent strains of oyster mushroom, lentinus edodes, black fungus, pholiota nameko, flammulina velutipes, hericium erinaceus and lucid ganoderma.

The invention discloses a method using pleurotus nebrodensis waste to cultivate flammulina velutipes. A formula of stock culture comprises 200 grams of peeled potatoes, 20 grams of glucose, 20 grams of agar, 3 grams of monopotassium phosphate (KH2PO4), 1 gram of magnesium sulfate (MgSO4), 150 micrograms of vitamin B and 1000 milliliters of water. A formula of stock comprises 98% of wheat berry and 2% of land plaster. A formula of cultigens comprises 98% of cotton seed hulls, 1% of sugar and 1% of land plaster. The rate between materials and water is 1:1.2 to 1.5. A formula of cultivation bags comprises 45% of fungi bran, 45% of cotton seed hull, 8% of corn powder, 1% of sugar and 1% of land plaster. The rate between materials and water is 1:1.2 to 1.5. The method can effectively utilize a lot of nutrition contained in the pleurotus nebrodensis waste to enable the pleurotus nebrodensis waste to be turned into wealth and effectively reduces production cost.

The present invention discloses a preparation method for increasing antioxidation activity of flammulina velutipes polysaccharide, wherein an enzymolysis control method is adopted to regulate and control molecular weight distribution of flammulina velutipes polysaccharide, flammulina velutipes is sequentially subjected to crushing, impurity removing, hot water extraction, protein removing, concentration and ethanol fractional precipitation separation to obtain the flammulina velutipes polysaccharide, and an enzyme method is adopted to carry out degradation control so as to reduce a molecular weight of the flammulina velutipes polysaccharide to 19000-30000 Da. The flammulina velutipes polysaccharide prepared by using the method is suitable for industrial production, wherein a polysaccharide extraction rate is increased by 2.5-3.5 times compared with a polysaccharide extraction rate of the traditional water extraction method, significant antioxidation activity is provided, IC50 of the flammulina velutipes polysaccharide in a DPPH.radical system is 1.69-2.12 mg/mL, and an ORAC value can be up to 923 mumol Trolox/g.

The invention relates to the field of biological engineering, in particular to a culture medium and a preparation method of the culture medium. The formula of the culture medium for a yellow flammulina liquid strain comprises raw materials and an aqueous solution, wherein the raw materials comprise the following components in percentage by weight: 1.5-2.5 percent of white granulated sugar, 0.2-0.5 percent of soy bean meal, 0.1-0.3 percent of deactivated yeast powder, 0.05-0.1 percent of magnesium sulfate and 0.05-0.1 percent of monopotassium phosphate. The preparation method of the culture medium for the yellow flammulina liquid strain comprises the steps of culture medium configuration, adjusting, sterilizing, inoculating and culturing. Due to the adoption of the technical scheme, after the culture medium for the yellow flammulina liquid strain, which is disclosed by the invention, is used, yellow flammulina mycelia have high growth speed, strong activity and high mycelium content; and the inoculated strain has the advantages of high germination rate, short fermentation period and high rate of finished products.

The invention discloses instant freeze-dried fungus soup which takes pleurotus eryngii, lentinus edodes, pleurotus ostreatus, flammulina velutipes, cordyceps militaris and eggs as main materials, and is prepared by the following steps: washing the pleurotus eryngii, the lentinus edodes, the leurotus ostreatus, the flammulina velutipes and the cordyceps militaris, removing roots, and cutting into sections; preparing hot fungus soup; cooling the hot fungus soup in air and putting the cooled soup into a freezing plate; putting the freezing plate into a quick-freezing tunnel and freezing into a soup ice block; putting the soup ice block into a vacuum bin with the vacuum degree less than 100Pa, and performing dehydrating and drying; when the water content of an entity is less than 3%, obtaining a freeze-dried dehydrated soup ice block, namely the instant freeze-dried fungus soup. The instant freeze-dried fungus soup has the advantages of being rich in nutrition, delicious in taste, convenient and quick to eat and beneficial to health.

The invention relates to a rice aroma beef sauce and a preparation method thereof. The rice aroma beef sauce is characterized in that the rice aroma beef sauce is prepared from the following raw materials by weight: 50-55 parts of beef, 2-2.4 parts of creeping liriope, 1-1.2 parts of equisetum hyemale l, 0.8-1 part of american ginseng, 1-1.2 parts of rehmannia glutinosa(gae-rtn.) libosch, 0.9-1 part of gerbera anandria (linn.)sch.-bip, 1.1-1.4 parts of monochoria vaginalis, 3-4 parts of propolis, 7-8 parts of hulless barley powder, 3-4 parts of capsella bursa-pastoris, 4-5 parts of flammulina velutipes, 4-4.5 parts of garlic sprouts, 22-25 parts of sunflower seed kernel, 30-35 parts of glutinous rice, 10-11 parts of crab meat, 0.1-0.2 part of a composite flavor protease, 350-370 parts of sweet flour paste, and 4-5 parts of a nutritional additive. According to the present invention, the aroma and the sweet taste of the glutinous rice and the delicious taste of the crab meat are fused into the rice aroma beef sauce, such that the rice aroma beef sauce has the unique flavor; the rice aroma beef sauce is rich in nutrition, wherein the hulless barley powder is rich in dietary fibers so as to remove toxins from the body, and the garlic sprouts contain capsaicine so as to provide effects of sterilization and influenza prevention; and the rice aroma beef sauce further contains a variety of Chinese herbal ingredients so as to provide effects of lung moisturizing, heart-fire clearing, eyesight improving, nebula removing, qi reinforcing and yin nourishing after regular taking.

The invention relates to a pleurotus eryngii culture medium, belonging to the technical field of edible fungus culture. The culture medium comprises the following components in parts by weight: 25-35 parts of mulberry wood dust, 25-35 parts of corn cob, 13-18 parts of bagasse, 8-12 parts of corn flour, 11-15 parts of rice bran, 1-3 parts of lime, 5-10 parts of xylitol slag, 0.5-1 part of calcium magnesia phosphate fertilizer, 8-12 parts of pecan shell, 3-8 parts of Flammulina velutipes mushroom residue and 0.01-0.1 part of fermentative bacteria strain. By using the mulberry branches and rice bran instead of the poplar wood dust, cotton seed hull, wheat bran and other raw materials, the pleurotus eryngii culture medium adjusts measures to local conditions, implements cyclic utilization of wastes in agriculture and forestry, lowers the pleurotus eryngii production cost, rationally utilizes the resources, implements energy saving and emission reduction, and has important meanings in implementing circular economy.