31 results about "Flammulina" patented technology

The invention belongs to the technical field of gene editing, and particularly relates to a construction method and application of a needle mushroom CRISPR / Cas9 gene editing vector. The CRISPR / Cas9 gene editing vector for flammulina velutipes is characterized in that the vector takes pFgnpt as a skeleton vector, and further comprises a Cas9 expression cassette and an sgRNA expression cassette; the nucleotide sequence of the Cas9 expression cassette is as shown in SEQ ID NO. 10. The nucleotide sequence of the sgRNA expression cassette is as shown in SEQ ID NO. 11. According to the present invention, with the agrobacterium-mediated transformation technology, the stable expression of Cas9 and sgRNA in the flammulina velutipes body is achieved, the efficient and stable CRISPR / Cas9 gene editing system is established, and the technical support is provided for the flammulina velutipes variety improvement, the functional gene research and the industrial upgrading.

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The present application provides an automatic picking device for Flammulina velutipes, comprising: a material frame conveying device for conveying the material frame from a growing workshop to a position to be picked; a positioning and clamping device arranged on the conveying device for positioning and clamping the material frame at the position to be picked; a first grabbing assembly comprising a pair of arc-shaped clamping jaws, the arc-shaped clamping jaws being opposite to clamp a cylindrical object and being capable of clamping the Flammulina velutipes and the culture medium apart; a second grabbing assembly comprising oppositely rotatable mechanical fingers, the mechanical fingers being capable of clamping a flaky cylinder to separate the cylindrical mushroom-containing piece from the Flammulina velutipes; and a mushroom-containing piece conveying device for conveying the separated cylindrical mushroom-containing piece to a storage and arrangement part. The device can automatically pick the cylindrical mushroom-containing piece from the bottle opening of the culture bottle and realize automatic picking, weight separation, harvesting and the like of the Flammulina velutipes, optimizes the operation process and helps to improve the growing efficiency.

The invention discloses application of a flammulina velutipes transcription factor gene AreA in regulation and control of cell wall polysaccharide synthesis and sporocarp taste. Flammulina velutipes as an important edible mushroom is widely welcomed due to rich nutrition, but the edible experience of consumers is seriously influenced and the market potential of the flammulina velutipes is limited due to the problems that the flammulina velutipes is poor in chewiness, easy to plug teeth and the like. The silent strain of the enoki mushroom areA gene is successfully constructed through an RNA interference technology, it is found that the polysaccharide content of cell walls and the toughness of sporocarp can be reduced through reduction of gene expression, then the taste of the sporocarp is improved, and the market competitiveness of the sporocarp is improved. According to the invention, the areA gene in the flammulina velutipes is effectively silenced through the specifically designed siRNA fragment, so that the polysaccharide content of the cell wall and the toughness of the sporocarp are reduced, and a theoretical basis is provided for reducing the toughness of the cell wall of the flammulina velutipes by using a genetic engineering technical means.

The invention provides a tablet candy capable of inhibiting fat absorption and a preparation method of the tablet candy, and relates to the technical field of functional foods. The pressed candy capable of inhibiting fat absorption comprises 0.1-25 parts of flammulina velutipes powder, 1-18 parts of L-arabinose, 0.05-5 parts of carob gum, 0.1-12 parts of chestnut powder, 0.1-10 parts of seaweed meal, 0.1-10 parts of instant oolong tea powder, 1-15 parts of composite vegetable powder, 30-80 parts of sorbitol, 3-15 parts of whole milk powder, 0.1-10 parts of edible mushroom concentrated powder, 1-10 parts of yoghurt powder, 1-15 parts of juicy peach juice powder and 0.1-2 parts of citric acid. And 0.1 to 1.5 parts of magnesium stearate. Various functional components such as beta-glucan and L-arabinose in the flammulina velutipes have a synergistic effect, so that the fat adsorption rate is increased; by means of the cold granulation and low-temperature tabletting technology, the retention rate of active ingredients is increased, meanwhile, the product has excellent stability and safety and good taste, the effects of improving intestinal flora and regulating blood sugar can be achieved, and important technical value and wide application prospects are achieved.

The invention discloses a camellia antler SCR0319T2 strain and an application of the camellia antler SCR0319T2 strain. According to the invention, a strain of camellia antler SCR0319T2 is collected and screened from the field, molecular biology identification shows that the strain belongs to Flammulina filiformes, subculture shows that the strain has excellent stability, can still grow stably after more than 30 times of passage, is low in elimination rate, can be used as an industrial large-scale production strain, and is high in strain running speed and short in production period. Through detection, the content of vitamin B1 and nicotinic acid in sporocarps is higher than that of common varieties, and mushroom bodies are durable in storage and good in taste.

This invention discloses an edible aerogel derived from *Flammulina velutipes*, its preparation method, and its applications. The preparation method includes the following steps: dispersing dried *Flammulina velutipes* powder in an aqueous sodium hydroxide solution, reacting, separating, and washing to obtain a precipitate; dispersing the precipitate in an aqueous sodium hypochlorite solution for bleaching, separating, washing, and freeze-drying to obtain an extract; dissolving polyphenols in an aqueous NaOH solution to obtain a NaOH / polyphenol dissolution system; dispersing the extract in the NaOH / polyphenol dissolution system, and shaking to promote CGC swelling; cycling the obtained suspension through freeze-thaw cycles 2-4 times; centrifuging the suspension, and partially dialyzing the solution to remove NaOH to obtain a hydrogel-like CGC-polyphenol complex; freeze-drying and grinding the hydrogel-like CGC-polyphenol complex to obtain an edible aerogel derived from *Flammulina velutipes*. This invention provides the application of the aforementioned edible aerogel derived from *Flammulina velutipes* in the preparation of weight-loss drugs or dietary supplements.

The invention relates to a white flammulina velutipes F523 with the preservation number of GDMCC No.67335. The white flammulina velutipes F523 is high in biological efficiency, short in growth cycle and stable in strain cultivation character, can be stably inherited and is used for flammulina velutipes cultivation and breeding. The invention also relates to an identification method of the flammulina velutipes F523, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether the amplification product has a band with a specific size so as to identify the F523. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention discloses an early-maturing flammulina velutipes variety and application thereof in flammulina velutipes breeding, and belongs to the technical field of flammulina velutipes molecular breeding. According to the invention, a new germplasm is created by using a CRISPR-Cas9 gene editing technology, and the fruiting phenotype of the strain is finally changed by changing a key gene by using the gene editing technology, so that a new variety 'Shangjin No.2' is created. The new variety at least has the following advantages: the yield is high; stipes are long, pilei are internally buckled and thick, and the appearance quality is good; the health-care food has the characteristics of high asparagine content, high glutamine content, high polysaccharide content and high cis-linoleic acid content, and is high in nutritional value; the method has the advantages of short growth period and prematurity. According to the invention, a domestic leading edible mushroom precise breeding technology platform is established, and the method has important strategic significance for improving the core competitiveness of the edible mushroom industry in China.

The utility model discloses a needle mushroom matrix fermentation bed which comprises a bottom plate, a box body is arranged on the bottom plate, a stirring groove is formed in the box body, a motor is arranged on the stirring groove, a group of cams are arranged at the output end of the motor, the output end of the motor is in butt joint with a stirring rod, and the other group of cams are connected with the stirring rod. Before use, raw materials and added substrates are poured into the screen plate on the box body, the scattered raw materials and substrates fall into the stirring tank from holes of the screen plate, the clustered raw materials or substrates remain on the screen plate, and then the motor drives the cam connected to the output end to rotate, so that the raw materials and the added substrates can be uniformly stirred. The cam is in sliding fit with two groups of wheel bodies on the arc-shaped plate, so that the net plate can longitudinally move above the stirring tank to form jolting, the clustered raw materials or substrates are dispersed by vibration and fall into the stirring tank, and then the stirring blades on the stirring rod can be used for stirring more uniformly, so that the stirring efficiency is improved.

The present application relates to the technical field of edible mushroom cultivation, and particularly relates to a flavedo-based flammulina velutipes culture medium and a preparation method thereof, wherein the raw materials comprise, by weight, 15-40 parts of citrus flavedo, 12-25 parts of wood chips, 10-20 parts of bran, 6-8 parts of bean cake powder, 1.5-2.5 parts of gypsum, 1.5-2 parts of sucrose, 0.3-0.8 parts of potassium dihydrogen phosphate, 0.2-0.3 parts of magnesium sulfate, 0.1-0.25 parts of bacillus subtilis and water, and the water is added to make the water content of the mixture reach 62%-67%. The flavedo-based flammulina velutipes culture medium and the preparation method thereof provided by the present application realize efficient resource utilization of fruit and vegetable flavedo waste, reduce the environmental problems caused by biomass waste, replace part of the traditional wood chip raw materials with flavedo, greatly reduce the raw material cost of the culture medium, significantly improve the economic benefits of flammulina velutipes cultivation, and are consistent with the development concept of agricultural circular economy.

The utility model discloses flammulina velutipes packaging equipment which comprises a carrying table, three lower limiting blocks are installed on the upper surface of the carrying table, two first electric push rods are installed on the upper surface of the carrying table, a movable plate is jointly installed at the telescopic ends of the two first electric push rods, three upper limiting blocks are installed on the bottom face of the movable plate, and the upper limiting blocks are installed on the bottom face of the movable plate. And a connecting frame is mounted on the bottom surface of the carrying table. According to the device, three lower limiting blocks and three bagging cylinders are arranged, two first electric push rods are used for driving a moving plate and three upper limiting blocks to move downwards, and meanwhile a second electric push rod is used for pushing a mounting plate, three connecting rods and three push discs to move forwards; according to the flammulina velutipes packaging device, the flammulina velutipes can be clamped, pushed out and packaged in a limited mode, operators do not need to place the flammulina velutipes frequently, the packaging efficiency of the flammulina velutipes is improved, and the flammulina velutipes packaged on the conveying belt can be automatically conveyed and discharged through the arrangement of the conveyor.

The application discloses a strain of Flammulina velutipes, a fermentation liquor of the strain and application thereof, and belongs to the technical field of microorganisms.The Flammulina velutipes strain R12 is preserved in the Guangdong Provincial Microbial Culture Collection Center on August 18, 2025, the address of the preservation center is No. 59 Building, 5th Floor, Guangzhou Xianlie Middle Road 100, and the preservation number is GDMCC NO. 66855.The fermentation liquor of the Flammulina velutipes strain R12 disclosed by the application has a specific bacteriostatic effect on vancomycin moderately drug-resistant Staphylococcus aureus XN108, and provides an important candidate substance for development of a new type of anti-Staphylococcus aureus drug.

The application discloses a white flammulina velutipes strain ZJJZG003 with early maturity, high amino acid content and high brittleness, an InDel marker for identification and application of the strain, and relates to the technical field of edible mushroom breeding. Flammulina filiformis The strain is obtained by crossing F24 and W1, has a faster growth speed, and has a mycelium growth speed of 5.63 mm / d at 30 DEG C and a growth speed of 4.75 mm / d at 25 DEG C, which are much higher than the growth speeds of the parents, and the time for primordium appearance is shortened.

The application discloses a flammulina velutipes variety X129, an identification method and application thereof. The flammulina velutipes variety X129 is preserved in the China General Microbiological Culture Collection Center (address: No. 3, Beichen West Road, Chaoyang District, Beijing, China) on June 6, 2025, and the biological preservation number is CGMCC No. 42022. The flammulina velutipes variety X129 provided by the application has a thick and non-opening cap, a thick and strong stem, few nodes, small pores in a transverse section and a white cut root.

The invention relates to a yellow flammulina velutipes C53 with a preservation number of GDMCC No.67334. The yellow flammulina velutipes C53 has the advantages of high yield, short growth cycle, more edible parts of stipes, difficulty in browning of stipe bases, long preservation time and stable inheritance, and is used for cultivation and breeding of flammulina velutipes. The invention also relates to an identification method of the flammulina velutipes C53, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether an amplification product has a band with a specific size so as to identify the C53. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

This application discloses a high-resistance, high-brittleness yellow enoki mushroom (ZJJZG002), InDel marker, and their application, relating to the field of edible fungi cultivation technology. The preservation number is CGMCC NO.41174, and the classification name is *Volvariella fuciformis* (also known as 'Silky Cap Winter Mushroom'). Flammulina filiformis The strain ZJJZG002 was obtained by using yellow enoki mushroom F24 and white enoki mushroom W1 as parents. After treating the parental materials with ultraviolet mutagenesis, single spore isolation, hybridization (hybrid combination XB51×YU19), and fruiting tests were carried out. The strain ZJJZG002 does not open its cap easily, has a strong mushroom aroma, a crisp taste, and uniform fruiting. It is suitable for various cultivation methods and can inject new seed sources into the domestic enoki mushroom market.

The invention provides streptomyces albidoflavus and application thereof, and relates to the technical field of microorganisms. The streptomyces alboflavus disclosed by the invention is named as Streptomyces alboflavus LY7, and the preservation number of the streptomyces alboflavus LY7 is CGMCC (China General Microbiological Culture Collection Center) No.37530. The strain has an inhibition effect on various phytopathogens, and has great potential in agricultural applications such as plant disease control. Related application and products of the biocontrol bacterium streptomyces albidoflavus, such as multifunctional bacterial manure taking needle mushroom bran as a matrix, are expanded, and the streptomyces albidoflavus has obvious bacteriostatic activity on main soil-borne disease pathogenic bacteria of ginseng, and can effectively prevent and treat root diseases of ginseng; the ginseng growth can be obviously promoted; the ginseng yield is increased. In addition, the microbial fertilizer can also effectively regulate the physicochemical properties of soil in the ginseng field, improve the enzyme activity of the soil and promote the healthy development of the ginseng industry.

The application discloses a Camellia japonica SCR0319T2 strain and application thereof. The Camellia japonica SCR0319T2 strain is collected and screened from the wild, and is identified by molecular biology to belong to Flammulina filiformis After subculture, it is found that the Camellia japonica SCR0319T2 strain has excellent stability, can stably grow after more than 30 generations, has a low elimination rate, can be used as an industrial large-scale production strain, and has a fast spawn production speed and a short production cycle. After detection, it is found that the fruiting body has higher contents of vitamin B1 and nicotinic acid than common varieties, is resistant to storage, and has a good taste.

The application discloses a kind of based on flammulina velutipes polysaccharide and brown alga polyphenol synergic high pressure electrostatic field auxiliary frozen and thawed aquatic product technology, belongs to food processing technical field.The application is aimed at the problems such as juice loss, texture deterioration, color and flavor decline and single physical field regulation limitation in the process of frozen and thawed aquatic product, and uses the synergic scheme of composite impregnation pretreatment combined with high pressure electrostatic field physical auxiliary.First, aquatic product is impregnated with flammulina velutipes polysaccharide and brown alga polyphenol composite solution, then frozen to center temperature-5 DEG C or lower under 4~7kV high pressure electrostatic field, after-18 DEG C storage, thawed to target temperature under the same electric field intensity.The method can refine ice crystal, reduce cell mechanical damage, inhibit lipid oxidation and microbial reproduction, significantly reduce juice loss rate, improve product elasticity, chewiness and color stability, adapt to clam meat, fish meat and other aquatic products.

The invention discloses a goat non-grain protein compound functional feed, belongs to the technical field of animal feeds, and aims to solve the problems that the traditional goat daily ration depends on high-price protein raw materials such as soybean meal, the cost is high, anti-nutritional factors exist, agricultural by-products such as macadamia nut green peel are not effectively utilized, and the feed utilization rate is low. The technical problems of complex fermentation of single strain or simple strain combination, low raw material efficiency, incomplete degradation of antinutritional factors, easy contamination by infectious microbes and the like are solved. The feed comprises a beer residue mixed fermentation material, a needle mushroom mycelium high-protein raw material, a jasmine flower residue mixed fermentation material and other raw materials in specific parts by weight, beer residues and oat hulls are fermented according to the ratio, the needle mushroom mycelium high-protein raw material is prepared by inoculating needle mushroom strains with a specific culture medium, and jasmine flower residues and oat hulls are fermented according to the ratio. The feed also contains a premix and a mineral nutrient additive, and efficient utilization of the raw materials is realized through multi-strain synergistic fermentation and fungal conversion; the feed is mainly used for goat breeding, and can reduce feed cost and improve goat growth performance and feed utilization efficiency.

The invention discloses a floating type intelligent identification root cutting device for flammulina velutipes. The floating root cutting device is composed of a grabbing device, a root cutting module, a root protecting module and a floating module. The grabbing device is used for grabbing flammulina velutipes, the root cutting module is used for cutting off the roots of the flammulina velutipes, the root protecting module is used for protecting the roots of the flammulina velutipes and fixing the positions of the roots, the floating module is used for achieving floating root cutting of the flammulina velutipes, and the camera module is installed on the floating module. The camera module is used for shooting and identifying roots of flammulina velutipes; and the root protection module can play a role in supporting the flammulina velutipes and effectively protect the roots of the flammulina velutipes, and can fix the flammulina velutipes, so that the root cutting efficiency and the root cutting stability are greatly improved.

The present application belongs to the technical field of edible mushroom planting, and particularly relates to a flammulina velutipes culture medium taking barley straw as main raw material and a preparation method thereof. The flammulina velutipes culture medium comprises barley straw powder, cotton seed hulls, bran, corn flour, lime, gypsum and a slow-release oxygen functional component. The particle size of the barley straw powder is 2-5 mm, and the barley straw powder accounts for 40%-60% of the total dry matter mass of the culture medium. The slow-release oxygen functional component is composed of a zeolite inner core loaded with calcium peroxide and a chitosan grafted polyacrylamide coating layer, and the coating layer has dual sensitivity to pH and temperature. The present application replaces part of the cotton seed hulls with barley straw as the main raw material, and the addition amount is as high as 40%-60%, which greatly reduces the cost of the culture medium, provides a new way for the resource utilization of agricultural waste, and has good environmental and economic benefits.

The invention provides low-GI functional honey as well as a preparation method and application thereof. The method comprises the following steps: preparing a honey culture medium by taking natural honey as a carbon source; the method comprises the following steps: inoculating edible mushrooms on a honey culture medium, fermenting, dynamically regulating and controlling fermentation conditions, centrifuging after fermentation, and carrying out ethanol precipitation, dialysis and freeze-drying on the obtained supernatant to obtain the low-GI functional honey, wherein the edible mushrooms are toadstool, aspergillus oryzae, chanterelle, shiitake mushrooms, pleurotus eryngii, flammulina velutipes or agaric. According to the method, monosaccharide in the honey can be converted into low-digestibility saccharides (such as sugar alcohol and oligosaccharide), meanwhile, the flavor of the honey is reserved, and the obtained low-GI functional honey is suitable for being used as food for auxiliary intervention of human diabetes, intestinal health management and sugar control of pets.

The present application relates to the technical field of health food, and in particular to a compound probiotic tablet for sleep aid and mood regulation, a preparation method thereof and application.The compound probiotic tablet comprises animal bifidobacterium lactis subspecies Probio-M8, animal bifidobacterium lactis subspecies Bbm-19, lactobacillus gasseri ProSci-108, lactobacillus gasseri G098, lactobacillus rhamnosus R7970, clostridium tyrobutyricum EC80, flammulina velutipes polysaccharide and excipients, synergistic effects between the flammulina velutipes polysaccharide and the strains are utilized, the types and the amount of the probiotics are reduced, the intestinal flora structure is reshaped, the synthesis of GABA and 5-HT is promoted, the central nervous system is regulated through the intestinal-brain axis, and the effects of sleep aid, mood regulation and improvement of skin water content are significantly improved.Meanwhile, the stability of the probiotic tablet is improved through improvement of the tablet excipients.

The invention provides a method for increasing the content of linoleic acid in soybeans through solid fermentation of edible fungi. Ganoderma lucidum G8, cordyceps sinensis No.1 and flammulina velutipes 738 are used as fermentation strains, and the soybean mycoplasm is obtained in a solid fermentation mode. The content of linoleic acid in the soybeans after solid fermentation of the edible fungus strain is greatly increased, the content of linoleic acid in the soybeans after solid fermentation of the lucid ganoderma G8 is increased to the maximum, the content of linoleic acid in the soybeans is increased to 132390.47 mg / kg from 59862.44 mg / kg before fermentation, the content of linoleic acid in the soybeans is increased by 72528.03 mg / kg, and the increase percentage is 121.16%; secondly, the content of linoleic acid in the soybeans is increased to 109452.56 mg / kg, the content of linoleic acid is increased to 49590.12 mg / kg, and the increase percentage is 82.84%; and then, the linoleic acid content in the flammulina velutipes 738 and the soybeans is increased to 104346.73 mg / kg and is increased to 44484.29 mg / kg, and the increase percentage is 74.31%. Therefore, edible fungus solid fermentation is an effective method for increasing the linoleic acid in the soybeans, and the linoleic acid content is increased by different amplitudes after solid fermentation of different edible fungus strains.

The invention discloses an early-maturing yellow flammulina velutipes variety and application thereof in flammulina velutipes breeding, and belongs to the technical field of flammulina velutipes molecular breeding. According to the invention, a new germplasm is created by using a CRISPR-Cas9 gene editing technology, and the fruiting phenotype of the strain is finally changed by changing a key gene by using the gene editing technology, so that a new variety 'Shangjin No.3' is created. The new variety at least has the following advantages: the yield is high; the stipe is longer, the color is golden yellow, and the appearance quality is better; the content of 19 amino acids is increased, especially the content of lysine, asparagine and glutamine is increased remarkably, the effect of increasing the content of polysaccharide and the content of cis-linoleic acid is also very obvious, and the nutritional value is very high; the method has the advantages of short growth period and prematurity. According to the invention, a domestic leading edible mushroom precise breeding technology platform is established, and the method has important strategic significance for improving the core competitiveness of the edible mushroom industry in China.

The invention relates to the technical field of flocculant development, in particular to a bio-based flocculant and a preparation method thereof.The bio-based flocculant comprises 15%-25% of cationic polyacrylamide, 23%-57% of polymeric silicate aluminum ferric sulfate, 6%-10% of heavy metal composite adsorption aid powder, 13%-23% of a magnetic adsorption aid, 7%-13% of composite bacterial powder, 2%-5% of a microbial deodorant, 4%-9% of a microbial oxygen aid and 2%-16% of an excipient. The heavy metal composite adsorption assistant powder comprises stenotrophomonas maltophilia, providencia alcaligenes and a mixture of metallophilic bacteria and lysinibacillus, the composite bacterial powder is a mixture of agaricus blazei hyphae, lentinus edodes mushroom residues, flammulina velutipes mushroom residues and oyster mushroom residues, and the microbial oxygen assistant is a mixture of cyanobacteria chlorophycocyanobacteria and zoogloea. On one hand, the flocculation speed and the decomposition speed of heavy metal impurities in sewage can be effectively increased, on the other hand, magnetic adsorption and rapid flocculation of the heavy metal impurities in the sewage can be achieved, and meanwhile, the flocculant can also achieve increase of the oxygen content of the sewage, so that the oxygen exposure purification efficiency during sewage flocculation is effectively improved.