The invention discloses an
arabidopsis pollen distant
signal gene AtSIPS and application thereof, and belongs to the technical field of
plant genetic engineering. The invention discloses an
arabidopsis pollen distant
signal gene AtSIPS and an application of a
protein coded by the
arabidopsis pollen distant
signal gene AtSIPS in regulation and control of distant hybridization reproductive disorder ability of cruciferous plants. The gene has
nucleotide sequences shown as SEQ ID No.1 and SEQ ID No.2. According to the invention, it is found for the first time that the AtSIPS gene is a distant signal in arabidopsis pollen, and the distant signal gene AtSIPS of the arabidopsis pollen is edited in a targeted manner in Col-0 ecological
arabidopsis thaliana by using a
CRISPR / Cas9 gene editing technology, so that an atsips1 / atsips2
mutant plant is obtained. The invention has the following advantages and positive effects: (1) the AtSIPS gene is determined as a distant signal in the arabidopsis pollen for the first time; (2) the
mutant pollen with the AtSIPS gene knocked out can germinate and grow on
capsella bursa-pastoris stigmas, and
hybrid ovules are obtained; (3) the discovery of the gene perfects the distant hybridization
molecular mechanism of the cruciferous plants, and provides a new idea for transferring ideal characters of wild relative species into the cruciferous plants in the future.