1447 results about "Rhamnose" patented technology

The invention discloses a preparation method for fast separating flavonoid glycosides from oil-tea-cakes with a medium pressure column, which comprises the following steps of: decorticating and crushing camellia oleifera abel seeds, degreasing the camellia oleifera abel seeds with non-polar solvents, performing extraction with ethanol water, filtering and condensing the extract to obtain crude extract concrete, performing fast separation with the medium pressure column to obtain an over 90 percent flavonoid glycoside mixture, and further adopting a high performance liquid chromatography to prepare over 95 percent flavonoid glycoside monomers, wherein the flavonoid glycoside monomers are kaemplerol 3-O-[2-O-beta-D-galactose-6-O-alpha-L-rhamnose]-beta-D-glucoside (I) and kaemplerol 3-O-[2-O-beta-D-xylose-6-O-alpha-L-rhamnose]-beta-D-glucoside (II) respectively. The method can be used for batch preparation, and has the advantage of providing quality raw materials for the development of flavonoid glycoside medicaments and healthcare functional products in the oil-tea-cakes.

The invention relates to a bacterium S2 for efficiently generating biosurfactant and a fermentation culture medium thereof, belonging to the field of biotechnology. The bacterium S2 is gram-negative bacterium which is rod-shaped, is (6-8)*(8-12) micrometers in size and has no spores and capsules. The bacterial strain belongs to the pseudomonas aeruginosa through identifying by morphological and physio-biochemical characteristics and molecular biology, and the collection code is CGMCC No.3034. The best fermentation culture medium is a novel culture medium which takes 50g/L of rapeseed oil as the only carbon source. The rhamnolipid is the only fermentation product of the bacterial strain and has high yield. The CMC (Critical Micelle Concentration) value of the fermentation liquid is 0.25 g/L; compared with the common chemical surfactant, the bacterium has more obvious emulsification and durability on hydrophobic substances, such as oils and the like.

The invention relates to a method for preparing icaritin through converting total flavones of epimedium by an enzyme method. The method comprises the step of converting multicomponent flavone glycosides in the total flavones of epimedium by a dual-enzyme system consisting of heat-resistant alpha-L-rhamnosidase and heat-resistant beta-glucosidase, so as to produce the icaritin. According to the method, multicomponent flavone compounds (such as icariin, Epimedin A, Epimedin B and Epimedin C) in the total flavones of epimedium can be almost completely converted into the target product; the optimal acting temperature is high, so that a substrate and intermediate products have good solubility, and a cosolvent is not required; and the enzymolysis time is short, and the yield is high. The icaritin prepared by the method has a remarkable higher propagation inhibiting effect on liver cancers, lung cancers, colon cancers and mammary cancers compared with each monomer of the total flavones of epimedium before conversion.

The invention discloses a rhamnolipid high-yielding bacterial strain, and applications thereof. The rhamnolipid high-yielding bacterial strain is named as pseudomonas aeruginosa KT1115, is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC M 2016686. The rhamnolipid high-yielding bacterial strain is stable in hereditary features and high in rhamnolipid yield, and is obtained via ARTP induced mutation, blue gel plate screening, and secondary screening. According to fermentation broth of pseudomonas aeruginosa KT1115, liquid surface tension is reduced to 24.8mN/m<2>, and emulsification value is as high as 80%. Optimization of fermentation conditions including carbon source and nitrogen source single factors, cheap crude glycerine and swill-cooked dirty oil are taken as a composite carbon source, so that production cost is reduced greatly. The rhamnolipid yield of pseudomonas aeruginosa KT1115 is 50.7g/L, is increased by 18.1 times compared with that of a starting strain, conversion rate is 0.75g/g, and is relatively high. Application prospect of pseudomonas aeruginosa KT1115 in further industrialized application is promising.

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: Bacillus subtilis AGTP BS3BP5, Bacillus subtilis AGTP BS442, B. subtilis AGTP BS521, B. subtilis AGTP BS918, Bacillus subtilis AGTP BS1013, B. subtilis AGTP BS1069, B. subtilis AGTP 944, B. pumilus AGTP BS 1068 or B. pumilus KX11-1, Enterococcus faecium ID7, Propionibacterium acidipropionici P169, Lactobacillus rhamnosus CNCM-1-3698, Lactobacillus farciminis CNCM-1-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a β-glucosidase (E.C. 3.2.1.21), a β-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof.

The invention discloses a method for preparing neohesperidin by an enzymic method, comprising the following steps: performing coordination between hesperidin and metal ions and dissolving in water, and realizing conversion from hesperidin to neohesperidin under the action of immobilized rhamnosidase and immobilized rhamnoside transferase. The method provided by the invention is adopted to solve the problem that production cost of neohesperidin widely used in medical, functional food and its cosmetic fields is high, and simultaneously opens up a new approach for recycling of industrial waste orange peel and high value-added utilization of hesperidin.

The invention discloses an effective part of stevia and the activity and the application thereof. The effective part mainly comprises stevioside category and flavone category which are obtained by extracting and separating from dried stevia leaves, wherein the sum of the percentage content of the stevioside components in the stevioside part is 5 to 100 percent (w/w), and the stevioside components mainly contains the stevioside, stevibioside, rebaudioside A, B, C, D, E, and F, dulcoside A and the derivative thereof, etc.; the sum of the percentage content of the flavone components in the flavone part is 5 to 100 percent (w/w), and the flavone components mainly contains luteolin, quercetin, luteolin-7-O-Beta-D-glucoside, apigenin-7-O-Beta-D-glucoside, quercitrin, quercetin-3-O-Beta-D-arabinoside, quercetin-3-O-(4-O-anti form-caffeoyl acyl-Alpha-L-rhamnose-(1 to 6)-Beta-D-galactoside) and the derivative thereof, etc., and 4, 5-dicaffeoylquinic acid and the derivative thereof, etc. The effective part has significant sugar-reducing and fat-reducing effects, can be used singly or combined with any other Chinese medicines and Western medicines or foods in any proportion, is used for preparing medicines or functional foods, and is used for treating hyperglycemia and hyperlipoidemia.

The invention discloses a method for separating and purifying a flavone compound of momordica grosvenori leaves by HSCCC and the product thereof, wherein, the method of the invention comprises the following steps: the grosvenori leaves are added with water or other polar solvents so as to carry out reflux extraction; the obtained extracts adopt the mixed solution of ethyl acetate, normal butanol and water as a solvent system and are separated and purified by a preparation-type high-speed counter current chromatograph, and then kaempferol-3-O-Alpha-L- rhamnose-7-O-[Beta-D-glucosyl group(1-2)-O-L-rhamnoside] and kaempferol-3,7-Alpha-L-2- rhamnoside. In the method, by applying the HSCCC method to separate and purify the extracts of the momordica grosvenori leaves, two flavone compounds with higher purity are successfully obtained by separation, thereby extending the source of the raw materials for flavone products; moreover, the method has simple preparation technique, easy operation and high purification efficiency, lays the solid first stone for extraction and utilization of the effective components in the momordica grosvenori leaves, and can avoid environment pollution caused by the waste and throw of a large amount of momordica grosvenori leaves.

The invention belongs to the field of health-care products, and particularly relates to a probiotics and Chinese herbal medicine compound preparation with a hypoglycemic function. The preparation is formed by mixing the components in parts by weight as follows: 25-30 parts of dioscorea roots, 15-25 parts of pueraria roots, 20-30 parts of cinnamon, 20-40 parts of lycium berries and 30-40 parts of freeze-dried probiotics powder, wherein probiotics comprises bacteria in percentage by weight as follows: 40% of bifidobacterium adolescentis, 40% of bifidobacterium longum, 10% of lactobacillus plantarum and 10% of lactobacillus rhamnosus. The preparation has the hypoglycemic function.

The invention discloses two types of flavonoid glycoside compounds which are kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(2'' ', 4'' '-O-diacetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-glucopyrose galactopyranoside and kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(4'' '-O-acetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-D-glucopyrose galactopyranoside respectively. The invention also discloses a method for preparing the two types of flavonoid glycoside compounds. Moreover, pharmacological tests prove that the two types of flavonoid glycoside compounds have quite good effects of reducing blood sugar and blood fat and resisting oxidation and cerebral ischemia, and thus, the flavonoid glycoside compounds of the invention can be used for preparing medicaments, food and cosmetics for preventing and treating diabetes, reducing blood fat and resisting oxidation and cerebral ischemia.

The invention relates to lactobacillus rhamnosus grx19 and its application. The collection number of the lactobacillus rhamnosus grx19 is CGMCC No.5519. The stain can be used to produce live bacteria lactobacillus beverages with long shelf-life and fermented dairy products. Lactobacillus rhamnosus grx19 is taken as culture starter alone or in combination with other lactic acid bacterias and then live bacteria lactobacillus beverages and fermented dairy products are prepared according to routine methods. After heat treatment at the temperature of 70-75 DEG C for 15-20 seconds, by the adoption of aseptic filling or sanitary filling, live bacteria lactobacillus beverages and fermented dairy products sold at room temperature can be produced.

The invention discloses aprobiotic solid beverage for infants. The probioticsolid beverage comprises components in percentage by weight as follows: 15-25% of bifidobacterium powder, 15-25% of lactobacillus rhamnosus powder, 10-30% of rice protein powder, 10-20% of bovine colostrum powder, 5-15% of maltodextrin, 5-10% of fructo-oligosaccharide and 5-10% of galactooligosaccharide. The probioticsolid beverage is natural in component, scientific in formula, balanced in nutrition, convenient to drink, free of caking, good in dissolving ability and unique in taste and has effects of improving intestinal tracts of the infants, improving digestion and enhancing the immunity.

The invention provides a green extraction technology for the comprehensive and efficient utilization of a Dioscorea zingiberensis resource. Saponins are extracted and simultaneously other effective components in Dioscorea zingiberensis, such as Dioscoreae starch, celluloses, a coronary heart disease control raw material, pectin, agricultural nucleic acids and the like, are comprehensively, efficiently and synchronously extracted through the technology. Rhamnose is extracted from a hydrolysis waste liquid; the whole process of the technology is green; and the technology has the advantages of great improvement of the saponin production level in China, improvement of the resource utilization efficiency, energy saving, environmental protection, realization of the unification of the economic benefit, the environmental benefit and the social benefit, and promotion of the sustainable development of Chinese economy and society.

The invention discloses a probiotic composition for relieving anxiety or depression and an application thereof. The probiotic composition comprises prebiotics, a K-KLJ probiotic combination and functional substances. The K-KLJ probiotic composition contains at least one of lactobacillus plantarum, lactobacillus reuteri, lactobacillus casei, and lactobacillus helveticus, or also contains at least one of lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacterium animalis and bifidobacterium longum. The probiotic composition of the present invention can significantly increase quantity ofbeneficial intestinal flora, improve intestinal discomfort, and reduce chronic inflammation, thereby reducing unhealthy emotion, and preventing emotional diseases such as depression and anxiety. Moreover, according to the invention, the combination of probiotic strains, the prebiotics, the probiotic strains and the functional substances have effect of significant synergistic enhancement.

The invention provides a method for preparing daily chemical microencapsulated probiotic. The method comprises the steps that: first, three probiotics which are lactobacillus acidophilus, lactobacillus plantarum and lactobacillus rhamnosus are fermented and centrifuged; such that wet cells are obtained; emulsifying, pre-freezing and freeze-drying are carried out; First embedding is carried out upon the obtained probiotic powder by using a crosslinked product of gelatin and TG enzyme; and second embedding is carried out by using a mixture of soluble starch, maltose and maltodextrin. With the method, the prepared microencapsulated probiotic has an embedding rate reaching 87%. The microencapsulated probiotic can be used in daily chemicals used for inhibiting acnes, relieving allergy symptoms, and lightening spots.