143 results about "Rhamnose" patented technology

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

The application discloses application of lactobacillus rhamnosus in preparation of a medicine for treating osteoporosis, relates to the technical field of biological medicine, and the osteoporosis is osteoporosis caused by estrogen deficiency; the medicine is used for inhibiting osteoclast differentiation to play the role of improving osteoporosis; the lactobacillus rhamnosus in the medicine is used for inhibiting the generation of active oxygen to inhibit osteoclast differentiation, thereby improving the osteoporosis caused by estrogen deficiency; the lactobacillus rhamnosus in the medicine is used for activating a nuclear factor Nrf2 signal path and downstream antioxidant enzymes to play an antioxidation stress role; the lactobacillus rhamnosus in the medicine is used for activating an Nrf2-mediated antioxidation path, inhibiting active oxygen generation, and then hindering osteoclast differentiation and function, thereby playing the role of improving osteoporosis; the lactobacillus rhamnosus provides a brand-new medicine treatment strategy for osteoporosis.

This application discloses a composite oil displacement system and its preparation method, belonging to the field of oilfield development technology. The composite oil displacement system provided in this application includes rhamnolipin and betaine surfactant, wherein the rhamnolipin is a biosurfactant obtained by culturing *Pseudomonas aeruginosa*. Both the rhamnolipin biosurfactant and the betaine surfactant are heat- and salt-resistant; therefore, the composite oil displacement system prepared using the rhamnolipin biosurfactant and the betaine surfactant has good compatibility with high-temperature, high-salinity reservoirs, meaning it is suitable for such reservoirs. Therefore, it can significantly improve the oil displacement effect in high-temperature, high-salinity reservoirs.

This invention discloses a modified rhamnolipin waterproofing agent for well workover operations in offshore oilfields and its preparation method. The method involves a one-step process where a 3-chloro-2-hydroxypropyltrimethylammonium chloride aqueous solution is added dropwise to a rhamnolipin solution, attaching a quaternary ammonium cation to the hydroxyl group of the rhamnolipin. After the reaction is complete and the solution is purified, the modified rhamnolipin waterproofing agent is obtained. The raw materials used in this invention are readily available, the preparation method is simple, and it is conducive to industrial production. The raw materials and products used have low biotoxicity and good environmental friendliness, which is beneficial for application in offshore oilfields. The solution prepared with the modified rhamnolipin waterproofing agent exhibits low surface tension, good temperature and salt resistance, and wetting improvement ability. It can significantly improve the reservoir water-locking permeability recovery rate, demonstrating not only a good waterproofing effect but also alleviating reservoir contamination problems caused by long-term production to a certain extent.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

A starter culture for the fermentation of a plant-based milk alternative is provided, which is particularly suitable for the production of a plant-based yogurt alternative. The starter culture comprises lactic acid bacteria isolated from legume plant material, especially pea plant material. Preferably, the starter culture comprises lactic acid bacteria of the species Lactobacillus rhamnosus and / or Lactococcus lactis subsp. cremoris.

This invention discloses a probiotic liquid core ball cultured under polysaccharide confinement, its preparation method, and its application in improving children's intestinal flora. Belonging to the field of probiotic preparation technology, it employs an extrusion method combined with calcium alginate cross-linking technology, using xanthan gum and other thickening polysaccharides to optimize the types and concentrations of polysaccharides to construct the confinement space. The effects of different oxygen conditions on the encapsulation of different proportions of *Lactobacillus rhamnosus* LRa05 and *Bifidobacterium animalis* BLa80 were investigated to obtain the optimal probiotic liquid core ball cultured under polysaccharide confinement. This process is simple, uses safe raw materials, and after confinement culture, the viable bacteria count is ≥10⁹ CFU / g. It can directionally increase the abundance of beneficial bacteria in children's intestines, inhibit opportunistic pathogens, enhance flora diversity and stability, and is suitable for children's dietary scenarios, providing a safe and efficient intestinal flora regulation solution.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

This invention discloses a strain of *Lactobacillus rhamnosus* H7 and its applications, belonging to the field of food microbiology technology. This invention provides a *Lactobacillus rhamnosus* H7 isolated from fermented meat, deposited at the Guangdong Provincial Microbial Culture Collection Center. This strain has the function of degrading nitrite. When inoculated into dried sausage, the residual nitrite and nitrosamine levels in the dried sausage are reduced, and the color of the meat product is significantly improved. When inoculated into fermented mandarin fish, the residual nitrite and nitrosamine levels in the fermented mandarin fish are also reduced. Therefore, inoculating fermented meat products with *Lactobacillus rhamnosus* H7 not only helps improve the safety of fermented meat products but also enhances their quality. This strain has good application prospects as a starter culture for fermented meat products.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

The present disclosure relates to a use of Lacticaseibacillus rhamnosus FMBL L23004 CNN in the preparation of a medicament for preventing or treating diabetes mellitus. The Lacticaseibacillus rhamnosus FMBL L23004 CNN was deposited with the China Center for Type Culture Collection (CCTCC) on 26 Jun. 2023 under the accession number CCTCC NO: M 20231099. The Lacticaseibacillus rhamnosus FMBL L23004 CNN strain has the effect of inhibiting the activity of α-glucosidase, α-amylase, and dipeptidyl peptidase IV. Administration of the strain can significantly lower oral glucose tolerance and insulin tolerance in Type 2 Diabetes Mellitus (T2DM) mice, reduce abnormal postprandial blood glucose fluctuations, decrease fasting blood glucose levels, lower fasting insulin levels, reduce blood lipid levels, and alleviate insulin resistance. The strain significantly improves the composition of the gut microbiome, increases the abundance and diversity of beneficial gut bacteria, and reduces the relative abundance of pathogenic bacteria.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The present application discloses a use of lactobacillus rhamnosus TCI366 and / or its metabolite, which is a use of lactobacillus rhamnosus TCI366 and / or its metabolite in preparation of a composition for improving skin condition, wherein the lactobacillus rhamnosus TCI366 is deposited in the German Collection of Microorganisms and the deposit number is DSM33290.

The application discloses a probiotic composition and a production process of a probiotic fermented bread crumb, and belongs to the technical field of bread crumb processing. The probiotic composition is a microcapsule structure containing malt dextrin, arabic gum and heat-denatured whey protein isolate as wall materials, and lactobacillus acidophilus and lactobacillus rhamnosus as core materials. The structure can ensure that a small amount of live bacteria and metabolic products are slowly released through the micropores of the wall materials in a normal temperature fermentation environment, so that the free lactic acid bacteria are prevented from rapidly proliferating, excessively producing acid and competing with yeast for carbon and nitrogen sources in the initial stage of fermentation. The probiotic composition can provide nutrition for the yeast in the fermentation process, promote cell division and metabolic activity of the yeast, and the polydextrose, inulin and arabic gum in the microcapsule wall materials are hydrophilic colloids, which can form a uniform hydration network in the dough.

This invention discloses a kidney-tonifying and liver-nourishing compound probiotic Cistanche fermented drink and its preparation method, comprising the following steps: mixing and sterilizing Cistanche deserticola, Poria cocos, malt, functional components, and natural selenium-strontium mineral water to obtain a fermentation substrate; activating *Lactobacillus casei*, *Lactobacillus acidophilus*, *Lactobacillus rhamnosus*, and *Lactobacillus plantarum* strains respectively, centrifuging and freeze-drying, and then mixing them to obtain a compound probiotic powder; culturing the compound probiotic powder in MRS medium to obtain a compound probiotic liquid; inoculating the compound probiotic liquid into the fermentation substrate for fermentation, sterilizing, and obtaining the kidney-tonifying and liver-nourishing compound probiotic Cistanche deserticola fermented drink. This invention, through the innovative integrated formulation technology of combining Cistanche deserticola, malt, and Poria cocos as medicinal and edible ingredients, multifunctional factor compounding, and compound probiotic fermentation, prepares a kidney-tonifying probiotic Cistanche deserticola fermented drink with high active ingredients, clear efficacy, and excellent flavor.

A Lacticaseibacillus rhamnosus MBP01 and a combination thereof for preventing and treating irritable bowel syndrome are provided, which relates to the technical field of microorganism. A deposit number of the Lacticaseibacillus rhamnosus MBP01 is GDMCC NO: 64725. The MBP01 can improve a weight loss trend of IBS model mice, increase food intake and feces water content, alleviate colon tissue damage, downregulate expression levels of IL-1β, TNF-α, PAR-2, and serum CORT and increase an expression level of IL-10. MBP01 with a high concentration can improve an expression level of OCC. Therefore, MBP01 has a certain positive regulatory effect on the intestinal function of IBS model mice and can effectively prevent and treat irritable bowel syndrome.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

This invention discloses a self-thickening, moisturizing, and oil-controlling gentle facial cleanser and its preparation method, belonging to the field of cosmetic technology. The raw materials are composed of the following percentages by weight: rhamnolipin 2%~8%, alkyl glycoside 5%~15%, cationic polymer 0.1%~1.5%, moisturizer 2%~6%, plant extract 0.5%~3%, pH adjuster (the amount needed to adjust the system pH to 5.0~7.0), preservative 0.1%~1.0%, and water as the balance. Through the ternary system of rhamnolipin-alkyl glycoside-cationic polymer, self-thickening and foam stability are achieved through electrostatic interaction and steric hindrance synergy, without relying on traditional thickeners such as carbomer. Simultaneously, it possesses excellent moisturizing and oil-controlling properties, with an oil removal rate ≥89%, a skin moisture content increase of ≥15% after 4 hours of use, and an oil secretion inhibition rate of ≥20% after 4 hours, with extremely low irritation. This invention solves the technical problem of low viscosity and poor foam performance in the rhamnolipin and alkyl glycoside compound system.

A sulfate-free personal care composition that includes a rhamnolipid surfactant and provides good cleaning and foaming properties. The rhamnolipid surfactant has a single rhamnose head group (i.e., mono-rhamno) and a single lipid tail group (i.e., mono lipid). The single lipid tail group has a carbon chain length of 10 to 13. The composition may include one or more additional surfactants selected from anionic surfactants, amphoteric surfactants, zwitterionic surfactants, non-ionic surfactants, cationic surfactants and combinations thereof, as long as the additional surfactant is free of sulfates. The composition also includes a dermatologically acceptable carrier.

The present invention relates to a Lactobacillus rhamnosus strain. In particular, the present invention relates to a composition comprising a Lactobacillus rhamnosus optionally in combination with a further lactobacilli and the use of the same in the treatment, prevention or alleviation of candidiasis.

The present application provides a pectin gel and a preparation method therefor. The pectin gel comprises pectin and a fat-soluble substance. The pectin comprises pectin. The weight-average molecular weight of the pectin is in the range of 5.0*10^4 Da to 6.0*10^5 Da, and the polydispersity index is 1 to 6. In the structural domains of the pectin, HG > 30 mol%, and RG-1 ≤ 35 mol%; HG is homogalacturonan, and RG-1 is rhamnogalacturonan type I. The emulsion stability of the pectin gel prior to molding is high. Thus, the molded pectin gel has good textural stability, low water syneresis and oil exudation, and a good mouthfeel.

A probiotic composition for treating or preventing metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma(MASLD-HCC) or colorectal cancer, comprises one or more microbiota selected from a group consisting of Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus GG, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis, Streptococcus thermophilus or any combination thereof. The probiotic composition demonstrates improved therapeutic effects when combined with low-dose Sorafenib for MASLD-HCC and outperforms 5-Fluorouracil in colorectal cancer models.

The present invention provides compositions and methods for treating patients. [Solution] The exemplary composition may include a vaginal microbiome composition. The vaginal microbiome composition may include a mixture of bacteria. The bacterial mixture may include Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, and Lactobacillus rhamnosus.

The application belongs to the field of admixtures, and particularly relates to a concrete thickening water-retaining agent and a preparation method and application thereof. The concrete thickening water-retaining agent is obtained by free radical polymerization reaction of an unsaturated acid monomer I, an unsaturated amide monomer, an unsaturated sulfonate monomer and a functional monomer; and the functional monomer is an esterification reaction product of rhamnolipid and an unsaturated acid monomer II. The key of the application is to introduce the esterification reaction product of rhamnolipid and the unsaturated acid monomer II as a copolymerization monomer into a copolymerization reaction system of the unsaturated acid monomer I, the unsaturated amide monomer and the unsaturated sulfonate monomer, so that the molecular structure of the concrete thickening water-retaining agent can be changed, the obtained concrete thickening water-retaining agent not only has excellent thickening and water-retaining properties, but also can improve the stability thereof in concrete.

The application discloses a 3D printing bacterial cellulose-based Lactobacillus rhamnosus / cottonseed peptide embedding material and a preparation method and application thereof. The method comprises the following steps: mixing sodium alginate and bacterial cellulose after shearing and homogenizing to obtain a sodium alginate-bacterial cellulose mixed solution, then adding Lactobacillus rhamnosus powder and cottonseed peptide to form a uniform suspension, and dropping the uniform suspension into a calcium chloride solution to solidify and form microspheres; another sodium alginate-bacterial cellulose mixed solution with high bacterial cellulose content is prepared as 3D printing ink, the microspheres are uniformly dispersed in the ink, and then 3D printing is performed, finally, a calcium chloride solution is sprayed on the surface of the printing product for secondary solidification, and a composite material with a double-layer embedding structure is obtained. The double-layer embedding system constructed by the bacterial cellulose and the sodium alginate can effectively protect and control the release of the Lactobacillus rhamnosus and the cottonseed peptide, and significantly improves the treatment effect of enteritis.

This invention discloses a primer-probe combination for detecting multiple types of Lactobacillus and its applications, belonging to the field of biotechnology. The primer-probe combination includes a primer-probe combination for detecting *Lactobacillus casei*, a primer-probe combination for detecting *Lactobacillus paracasei*, and a primer-probe combination for detecting *Lactobacillus rhamnosus*. The primer-probe combination of this invention can be used for both singleton and multiplex PCR detection, and can accurately detect *Lactobacillus casei*, *Lactobacillus paracasei*, and *Lactobacillus rhamnosus*, enabling the authentication of products containing Lactobacillus. This invention provides a more convenient method for detecting *Lactobacillus casei*, *Lactobacillus paracasei*, and *Lactobacillus rhamnosus*.

The application discloses a rehmannia polysaccharide, a preparation method thereof and application of the rehmannia polysaccharide in preparation of a medicine for preventing and treating kidney injury caused by cisplatin. The rehmannia polysaccharide contains the following monosaccharides in percentage by mass: rhamnose (Rha) 10.265%-14.963%, glucuronic acid (GlcA) 0.514%-0.904%, galacturonic acid (GalA) 35.784%-40.596%, glucose (Glc) 3.128%-5.048%, galactose (Gal) 37.892%-41.438% and arabinose (Ara) 3.869%-4.865%. The structure of the rehmannia polysaccharide is clear, and the rehmannia polysaccharide shows a significant improvement effect on cisplatin nephrotoxicity through cell experiments and animal experiments, and has important value for developing a new and effective prevention and treatment product of cisplatin-induced acute kidney injury.

The application relates to the field of biological medicine, and particularly relates to application of kaempferol-3-O-acacia di-saccharide-7-O-rhamnose glycoside in preparation of a medicine for preventing and / or treating diseases related to glycolipid metabolism disorder. The compound or a pharmaceutically acceptable salt, ester, solvate or prodrug thereof can be used for treating diseases such as type 2 diabetes, insulin resistance, hyperglycemia, hyperlipidemia and non-alcoholic fatty liver disease. The compound can significantly reduce intracellular glucose, triglyceride and total cholesterol levels, and can play a role by activating an AKT signal pathway, so as to improve glycolipid metabolism disorder and has potential clinical application value.

This invention discloses the application of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder in the preparation of immunomodulatory drugs, belonging to the field of bio-fermentation and immunomodulation technology. The *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is prepared by fermenting ginseng with *Lactobacillus rhamnosus*. The rare ginsenosides include at least one of Ck, Rk1, Rh4, and Rg5. The immunomodulatory drug is used to improve cyclophosphamide-induced immunodeficiency. The amount of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder added to the immunomodulatory drug is 10%-50% (mass fraction). The preparation method of the *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is as follows: S1, ginseng pretreatment; S2, strain activation; S3, fermentation culture; S4, freeze-dried powder preparation. This invention has the technical effects of high bioavailability of ginseng active ingredients, significant immunomodulatory effect, good safety, and effective improvement of cyclophosphamide-induced immunodeficiency.