719 results about "Legume" patented technology

The invention relates to a gene GmHB1a participating in development of vascular bundles of soybean root nodules and application of the gene GmHB1a. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, which comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, the plant is a leguminous plant, preferably soybean, and the nodulation number of the transgenic plant is changed. Optionally, the change in number of nodules is manifested as an increase in number of nodules and / or in number of single nodules, and optionally, the change in number of nodules is manifested as a decrease in number of nodules and / or in number of single nodules.

The invention relates to a coral sand soil multi-dimensional ecological low-carbon reinforcement method which comprises the following steps: S1, a urease solution is sprayed on a coral sand single-particle soil layer, a cementing liquid A is sprayed after standing, the urease solution is extracted from legume plants, and the cementing liquid A is obtained by mixing a urea solution and a calcium chloride solution; s2, on the basis of treatment in the step S1, a sporosarcina pasteurii bacterial solution is firstly sprayed, after standing, a cementing solution B is sprayed, the cementing solution B is alternately and circularly sprayed for multiple times, and the cementing solution B is obtained by mixing a urea solution and a calcium chloride solution; s3, a 3D printing geosynthetic material is laid on the basis treated in the step S2; and S4, laying a coral sand single-particle soil layer on the basis of treatment in the step S3, and repeating the steps S1 to S3. According to the method, a single particle size-foundation unit size-engineering size reinforcing system is constructed, comprehensive reinforcing from a microscopic single particle to a macrostructure can be achieved, and the reinforcing effect and efficiency are effectively improved.

The invention discloses a complex microbial inoculant for promoting growth of leguminous plants and application of the complex microbial inoculant, and relates to the technical field of fertilizers and biologication.The complex microbial inoculant is prepared from 25-35 parts of a microbial inoculant, 10-20 parts of a biological active agent, 10-15 parts of a biological activator and 30-55 parts of water. The endophyte 3G1 which is screened and separated from rice seeds and can promote the growth of leguminous roots can be completely and successfully planted in plants by utilizing the effects of preventing and treating plant diseases and / or promoting plant growth of microorganisms, and the growth of the plants is directly influenced; in combination with the functions of low temperature resistance, plant disease resistance and the like of other complex microbial inoculants, the compound microbial inoculants have growth promoting and disease preventing effects on leguminous plants; the biological active agent is lipopeptide, so that the spreading performance of plant leaves can be improved, the absorption of the complex microbial inoculant is promoted, the activity of the microbial inoculant is improved, and the crop yield is promoted to be increased by 3%; the biological activator can provide trace elements in the plant growth process, and is matched with the complex microbial inoculants for use, so that the growth of crop seedlings can be effectively promoted, the stress resistance of the crop seedlings is improved, and the transplanting survival rate of the seedlings reaches up to 98%.

The invention discloses a linked molecular marker of a major QTL (quantitative trait loci) uq2 of a peanut legume singulation character as well as an identification method and application of the linked molecular marker. The molecular marker and a major QTL site uq2 of the peanut legume singulation character are co-localized on a second chromosome of a peanut genome, and the molecular marker comprises molecular markers SNP4 and SNP57. The physical position of the molecular marker SNP4 is the 11591222th base position of the second chromosome of a cultivated peanut genome, and the polymorphism is C / T; the physical position of the molecular marker SNP57 is the 11894442 base position of the second chromosome of the cultivated peanut genome, and the polymorphism is A / T. The linked molecular marker is obtained by using a primer group. The nucleotide sequences of the primer group are respectively as shown in SEQ ID No. 1 to SEQ ID No. 6. The major QTL locus uq2 of the peanut legume constriction character as well as the linked molecular marker and the identification method of the major QTL locus uq2 are suitable for gene localization of the peanut legume constriction character and molecular marker-assisted selective breeding.

Soybean plants producing seeds and soybean seeds comprising increased protein content are provided. Also provided are soybean plants producing seeds and soybean seeds comprising increased amounts of essential amino acids such as methionine, tryptophan or lysine. Protein compositions having increased essential amino acids can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions having increased protein content or increased amounts of essential amino acids are disclosed.

A soybean cultivar designated 13380227 is disclosed. The invention relates to the seeds of soybean cultivar 13380227, to the plants of soybean cultivar 13380227, to the plant parts of soybean cultivar 13380227, and to methods for producing progeny of soybean cultivar 13380227. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 13380227. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 13380227, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 13380227 with another soybean cultivar.

Provided are a use of epicatechin or a legume extract containing epicatechin for preparing a composition for tranquilizing skin, and a skin itch treatment method comprising a step of applying a cosmetic composition to the skin of an individual causing itch, the cosmetic composition being the composition for tranquilizing skin.

The invention relates to natural plant hair dye, which comprises a bean extract, a lucid ganoderma extract, an astragalus extract, a polygonum multiflorum extract, vegetable fat, erythritol, L-proline, ferulic acid and gamma-aminobutyric acid, does not contain phenylenediamine, resorcinol, ammonium hydroxide, a vaccinium bracteatum thunb leaf extract and other components at all, and does not need to additionally add a thickening agent, a care agent and an alkalizing agent. The hair dye is good in hair dyeing effect, safe, reliable and non-irritant.

The invention discloses keratinase Phyto-Keratinase and application of the keratinase Phyto-Keratinase. The keratinase Phyto-Keratinase comprises the following components: (1) protease which is coded by a nucleotide sequence as shown in SEQ ID NO.1; and / or (2) protease which is derived from bacillus subtilis, is coded by a nucleic acid sequence with more than 98% of identity with the SEQ ID NO.1 nucleotide sequence and has the characteristic of hydrolyzing keratin. According to the plant keratinase Phyto-Keratinase disclosed by the invention, the Phyto-Keratinase (plant keratinase) produced by utilizing microbial fermentation is a serine proteolytic enzyme, is also a biocompatible cutinase, and is obtained by fermenting saccharomyces cerevisiae. Phyto-Keratinase provides a nitrogen source for the growth of plants (beans), and contains various amino acids (functional peptides) and special proteolytic enzymes derived from the plants at the same time. The Phyto-keratinase can provide the effects of resisting inflammation, removing cutin and providing nutrition for skin regeneration at the same time, can relieve various inflammations by inhibiting PAR-2 receptors, and has potential value in the fields of cosmetics, beauty medicine and environment.

The invention provides an additive-free high-protein nut mixed plant-based beverage and a preparation method thereof.The additive-free high-protein nut mixed plant-based beverage is prepared from, by weight, 10-50 parts of beans, 0-100 parts of white granulated sugar and 70-210 parts of nuts, and the ratio of the beans to the nuts is 1: (3-5); the preparation method comprises the following steps: mixing beans, nuts and water, and grinding to obtain a ground material liquid; heating the ground material liquid, adding white granulated sugar, and stirring at a constant temperature to obtain a mixed material liquid; performing primary protein hydration on the mixed feed liquid to obtain primary protein hydration feed liquid, and filtering to obtain filtered feed liquid; performing secondary protein hydration on the filtered feed liquid to obtain secondary protein hydration feed liquid, and homogenizing twice to obtain homogenized feed liquid; and sterilizing the homogenized feed liquid to obtain the product. The product is free of additives, mellow in taste, stable in state and free of bad states such as layering and precipitation in the shelf life.

A soybean cultivar designated 15301811 is disclosed. The invention relates to the seeds of soybean cultivar 15301811, to the plants of soybean cultivar 15301811, to the plant parts of soybean cultivar 15301811, and to methods for producing progeny of soybean cultivar 15301811. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 15301811. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 15301811, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 15301811 with another soybean cultivar.

The invention provides an MsLEA3 gene and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the alfalfa MsLEA3 gene is subjected to overexpression or gene editing is carried out on the MsLEA3 gene in alfalfa through a CRISPR / Cas9 system, so that an overexpression transgenic line of the alfalfa MsLEA3 gene or a gene editing transgenic line aiming at the alfalfa MsLEA3 gene is obtained. The salt tolerance of an overexpressed or gene-edited medicago sativa transgenic line of the MsLEA3 gene is remarkably improved, the MsLEA3 gene can be used for cultivating medicago sativa varieties with high salt resistance, a new theoretical basis and candidate genes are provided for cultivating new varieties of high-resistance leguminous forage, and the application of medicago sativa in production is greatly promoted.

The invention belongs to the technical field of plant allelopathy, and particularly relates to application of bean root exudates in allelopathy of hydroponic barley grass. On the basis of a hydroponic barley grass technology, a hydroponic wheat-bean mixed planting mode is created for the first time, allelopathy effects between different bean root exudates and hydroponic barley grass are mined and analyzed by surrounding bean root exudates and utilizing the allelopathy effects among organisms, specific responses of hydroponic barley grass to the bean root exudates in physiological and biochemical indexes are determined, and the method has a good application prospect. And identifying the physicochemical property change of the hydroponic barley grass. A novel method is provided for a novel efficient, safe and environment-friendly bacteriostatic mode of the hydroponic barley grass, a theoretical basis and an improvement direction are provided for developing a novel biological bacteriostatic agent and a hydroponic barley grass planting facility, and a hydroponic wheat and bean mixed planting mode is further promoted to become a planting technology for effectively improving green feed nutrition.

A soybean cultivar designated 10330716 is disclosed. The invention relates to the seeds of soybean cultivar 10330716, to the plants of soybean cultivar 10330716, to the plant parts of soybean cultivar 10330716, and to methods for producing progeny of soybean cultivar 10330716. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 10330716. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 10330716, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 10330716 with another soybean cultivar.

The present disclosure relates to low density, high protein, ready-to-eat food pieces, compositions including such food pieces, and methods of making such food pieces. The food pieces include at least 70% by dry weight protein, where the protein ingredients include a base protein blend that makes up at least 60% by weight of the protein ingredients. The base protein blend includes sodium caseinate and one or more of legume protein isolate, legume protein concentrate, milk protein isolate, or milk protein concentrate.

A threshing mechanism of the small-grain type bean combine harvester is used for separating grains from straw and threshing the grains, a screening mechanism is located below the threshing mechanism and used for receiving the grains, and a first receiving mechanism and a second receiving mechanism are sequentially arranged below the screening mechanism. The material screening mechanism enables grains to be conveyed from front to back through vibration, the grains which are successfully threshed leak the material screening mechanism and fall into the first material receiving mechanism, the first material receiving mechanism is connected with the grain box to collect the grains, and the grains which are not successfully threshed fall into the second material receiving mechanism from an outlet of the material screening mechanism. The second material receiving mechanism is connected to the threshing mechanism and is used for conveying unsuccessfully threshed grains back to the threshing mechanism for re-threshing operation; and the discharging fan works to provide wind power, so that the straws and the threshed shells are blown to the grass collector at the output end. The process of screening and threshing is mechanically completed, seed collection and impurity collection are completed, manual operation is replaced by a machine, the harvesting efficiency is improved, and the labor cost is reduced.

The invention discloses anthocyanin-enhanced sleep-aiding whole-bean black soybean milk and a preparation method thereof, and belongs to the technical field of food processing. The anthocyanin reinforced whole-bean black soybean milk is prepared by utilizing a high-energy fluid mill and comprises the following raw materials: 9-12 parts of black soybeans, 1-3 parts of mulberries, 1-3 parts of black Chinese wolfberry fruits, 73-86 parts of water, 0.5-2 parts of cane sugar and 0.01 part of maltodextrin. The preparation method comprises the following steps: finely grinding and crushing the raw materials by using a high-energy fluid mill in the processing process, homogenizing with auxiliary materials, and sterilizing. The black soybean milk is high in bean protein solubility and rich in anthocyanin and has the effects of soothing the nerves and helping sleep, the production process is easy to operate, the production period is short, and the black soybean milk has good application and popularization value.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIP5-GmPKM20 gene module in regulation and control of nodulation of leguminous plants, the GmNIP5-GmPKM20 gene module is one or more of GmNIP5 and genes interacting with the GmNIP5, by analyzing the influence of the transgenic GmNIP5 and the genes GmPKM20 interacting with the GmNIP5 on soybean nodulation, it is clear that the GmNIP5 promotes infection of nodule bacteria and formation of root nodules, and the GmNIP5-GmPKM20 gene module can be used for regulating and controlling nodulation of leguminous plants. GmPKM20 inhibits symbiotic nodulation signals of soybeans, and it is verified that GmPKM20 and GmPKM20 can directly interact with each other.

The present invention is directed at a method to produce a substantial number of seeds (e.g., 10,000) from a single plant. The method involves growing a mother plant under conditions designed to substantially maintain the mother plant in a vegetative state and substantially delay reaching reproductive stage. Then, removing the apical meristem once the mother plant has reached a desired stage of growth. The method further involves removing one or more branches from the mother plant (as branch cuttings). The method additionally involves maintaining each of the branch cuttings in growth media under acclimation conditions until each of the branch cuttings resume growing vegetatively as clone plants, then inducing the clone plants to flower and once the clone plants have been induced to flower, returning them to vegetative growth conditions. The clone plants are then maintained under vegetative growth conditions until they have produced a desired number of seeds.

The invention discloses application of a nodU gene in regulation and control of nodulation capability and nitrogen fixation efficiency of azotobacter stem nodule, and belongs to the technical field of biology. According to the research, the nodU gene is over-expressed in the azotobacter japonicus, so that the nodulation and nitrogen fixation capacities of the azotobacter japonicus can be improved at the same time. The nodulation gene nodU overexpression strain of the nodulation gene nodU of the stem nodulation nitrogen-fixing rhizobium ORS571 is constructed, the nodulation gene nodU overexpression strain is inoculated to a leguminous plant for symbiosis, and compared with the leguminous plant inoculated with wild type rhizobium, the nodulation number of the leguminous plant can be obviously increased, the nodulation nitrogen-fixing capacity is improved, and the plant growth phenotype is improved.

The invention provides a method for improving the productivity of degraded grazing grassland, and belongs to the technical field of grassland restoration. The method comprises the following steps that water and fertilizer automatic management devices are installed on the grazing grassland needing to be repaired every 2 m; the gramineous forage grass is sown in ditches, then the two kinds of leguminous forage grass are evenly mixed, and the mixed forage grass and the gramineous forage grass are sown in a cross mode; a wireless sensor system is arranged in the automatic water and fertilizer management device, the soil moisture content, the soil nutrient content and the vegetation growth condition are monitored through the wireless sensor system, and precise irrigation and precise fertilization are conducted according to monitoring data. By means of the measures of intelligent fertilization irrigation and no-tillage reseeding, the productivity of the degraded grassland is improved. The fertilization time, the use amount and the field moisture capacity of the grass on the grassland are strictly controlled, so that the absorption of the grass on the grassland to nutritional ingredients can be promoted, and the productivity of the grassland and the yield of the grass are further improved.

To provide a method for producing bean powder obtained from plants of the genus Mucuna including Mucuna pruriens, the method being capable of producing powder that maintains, as much as possible, the L-dopa content in beans obtained from plants of the genus Mucuna including Mucuna pruriens, and that inhibits discoloration due to heating and maintains the original white color of the beans.SOLUTION: A method for producing Mucuna bean powder, the method comprising a step for sterilizing beans (hereinafter referred to as "Mucuna beans") obtained from plants of the genus Mucuna including Mucuna pruriens (step S1), a step for washing the sterilized Mucuna beans (step S2), a step for dehydrating the washed Mucuna beans (step S3), a step for steam-heating the dehydrated Mucuna beans at 100-150°C for 5-30 minutes (step S4), a step for quickly cooling the Mucuna beans after steam-heating (step S5), and a step for crushing and drying the cooled Mucuna beans at 80-100°C (step S6).SELECTED DRAWING: Figure 1

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust and / or powdery mildew) in a plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

The invention relates to the technical field of microbial fermentation, and particularly discloses a microbial fermentation process for food processing. The composition used in the process comprises lactobacillus plantarum, aspergillus oryzae and a compound enzyme preparation containing neutral protease, cellulase and phytase. The preparation method comprises the following steps: firstly, pretreating the raw materials, then compounding lactobacillus plantarum and aspergillus oryzae into a fermentation starter according to a specific proportion, fermenting in a liquid environment, and implementing dynamic stepped oxygen control; adding a complex enzyme preparation in a hypha growth period; then transferring into solid matrix conversion to construct a porous structure; and finally, carrying out two-stage temperature-controlled vacuum drying to obtain the product. The composition disclosed by the invention can be used for fermenting plant-based raw materials such as beans and grains, and has the advantage that the availability of a substrate can be improved through the synergistic interaction of bacteria and enzymes; in addition, according to the process, through stepped oxygen control, the double-bacterium respiratory rhythm can be precisely adapted, and the problem of metabolism mutual exclusion in a constant oxygen mode is thoroughly solved.

The present application provides isolated regulatory genome sequences of legume plants, preferably Glycine, Pisum, Cicer plants, such as Glycine max, Pisum sativum, Vigna radiata, Vicia faba, Phaseolus vulgaris, Cicer arietinum, and uses thereof, as shown in SEQ ID NO: 3. The present application has important application value for developing close-planting soybeans, intercropped soybeans, etc., and has great value for optimizing the architecture of soybean plant type and improving the yield of soybeans per unit area related to theoretical and practical research.

The invention provides an Au-DTNB-Ag nanosphere, a preparation method and application of the Au-DTNB-Ag nanosphere in preparation of SERS (Surface Enhanced Raman Scattering) enhanced immunochromatography test paper. Wherein the Au-DTNB-Ag nanosphere comprises a nanogold core, a nanosilver shell and a self-assembly DTNB monomolecular layer located in the middle, the particle size of the nanogold core ranges from 16 nm to 18 nm, and the thickness of the nanosilver shell ranges from 17 nm to 18 nm. The invention also provides an SERS (Surface Enhanced Raman Scattering) immune probe using the nanosphere, DTNB in the Au-DTNB-Ag nanosphere is coupled with the rabbit-derived polyclonal antibody, and the invention also provides an application of the nanosphere or the immune probe in preparation of SERS enhanced immunochromatography test paper. The SERS technology is combined with the immunochromatography test paper, rapid and high-sensitivity quantitative detection and analysis of soybean allergen glycinin are achieved, actual sample detection of soybean products in daily life is achieved, a more convenient, rapid and efficient detection tool is provided for detection of allergens in food of allergic consumers, and the method is suitable for large-scale popularization and application. And reference opinions are provided for selection of consumers.

The invention relates to aspergillus oryzae sourced protease and application thereof in traditional bean fermentation products, and belongs to the technical field of enzyme engineering and microbial engineering. The amino acid sequence provided by the invention comprises an acid protease, two neutral proteases and an alkaline protease, wherein the proteases have higher degradation degree on globulin and albumin; the enzyme activity is the highest at 45-50 DEG C, and the salt tolerance is good. When the soybean sauce is prepared, the physicochemical indexes, free amino acids, organic acids and flavor substances of the soybean sauce are remarkably improved; wherein the quantity and the content of flavor substances are remarkably increased, and the content of free amino acid is increased by 20-25%; and the flavor sensory evaluation is more pleasant. In the early stage to the middle stage of fermentation, the content of amino acid nitrogen in the soy sauce is remarkably increased, the production cycle of the soy sauce can be shortened, and the method has wide application prospects in the food industry.

The invention provides a bean food frying device which comprises a frying box, a rotary drum capable of rotating around the axis of the rotary drum is arranged in the frying box, a screen frame capable of rotating along with the rotary drum is arranged in the frying box, and the face, away from the rotary drum, of the screen frame is communicated with a discharging pipe flush with one side in the screen frame. An arc-shaped plate covering the screen frame is fixed in the frying box, the two sections of the arc-shaped plate are located above and below the screen frame in the transverse state respectively, and the discharging end of the discharging pipe makes contact with the inner wall of the arc-shaped plate; a discharging opening is formed in the side, close to the lower section of the arc-shaped plate, of the frying box. According to the broad bean oil draining device, broad beans can effectively and repeatedly roll during oil frying and oil draining, the situation that the broad beans are in a static state all the time is avoided, it is guaranteed that all the broad beans can be fully fried, the follow-up broad bean oil draining efficiency is improved, oil can be thoroughly drained from the broad beans, and the broad bean oil draining efficiency is improved. And meanwhile, workers do not need to manually discharge the broad beans after oil draining, so that the processing efficiency of the broad beans is improved.

Aspects of the present disclosure relates to genetically altered LysM receptors. In particular, the present disclosure relates to a hydrophobic patch into the LysM2 domain which can increase affinity and / or selectivity for LCOs and by replacement of regions in the LysM1 domain with the corresponding regions of the LysM1 domain from a donor LysM receptor that can alter the affinity and / or selectivity for the oligosaccharide particularly for LCOs and can alter the specificity between LCO when using regions from a high affinity and specificity LCO LysM receptor such as a legume NFR1 receptor. The present disclosure also relates to genetically altering LysM receptors in plants to include a hydrophobic patch or alter the hydrophobic patch and to genetically altering LysM receptors in plants by replacement of regions in the LysM2 domain.

A soybean cultivar designated 13150624 is disclosed. The invention relates to the seeds of soybean cultivar 13150624, to the plants of soybean cultivar 13150624, to the plant parts of soybean cultivar 13150624, and to methods for producing progeny of soybean cultivar 13150624. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 13150624. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 13150624, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 13150624 with another soybean cultivar.