283 results about "Isoflavones" patented technology

The invention belongs to the technical field of microbial fermentation, and particularly relates to a plant lactobacillus XD117, application of the plant lactobacillus XD117 in fermentation of astragalus membranaceus, an astragalus membranaceus fermentation product and a preparation method of the astragalus membranaceus fermentation product. The bacterial strain is lactobacillus plantarum XD117 and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacterial strain is CCTCC NO: M 20241730. The preparation method of the astragalus mongholicus fermentation product comprises the following steps: inoculating the lactobacillus plantarum XD117 into astragalus mongholicus enzymatic hydrolysate, and fermenting in a fermentation temperature range suitable for the lactobacillus plantarum XD117 to obtain the astragalus mongholicus fermentation product. The total content of calycosin, formononetin, calycosin glucoside and formononin in the astragalus mongholicus fermentation product is high, and the astragalus mongholicus fermentation product has a very good antioxidant effect.

The invention provides application of a soybean GmWRKY40 gene in soybean breeding, and belongs to the technical field of functional gene breeding. The nucleotide sequence of the soybean GmWRKY40 gene provided by the invention is as shown in SEQ ID NO: 1. According to the invention, the GmWRKY40 gene is constructed into a skeleton expression vector to obtain a recombinant vector, and the recombinant vector is transferred into soybean under the mediation of agrobacterium tumefaciens to obtain a transgenic plant with stable overexpression of the GmWRKY40 gene. Detection results show that overexpression of the GmWRKY40 gene can significantly improve the grain isoflavone content and the protein oil content of the transgenic soybean, improve the soybean quality and increase the soybean grain yield at the same time. Therefore, the soybean GmWRKY40 gene can be used for developing new varieties of high-quality soybeans.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and an engineering-modified biological host. The invention discloses a series of glabridin synthesizing enzymes (4 '-O-methyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase and demethylase) from glycyrrhiza glabra for the first time, and the glabridin synthesizing enzymes have corresponding activities in various biological hosts. The heterologous biosynthesis of the glabridin is realized for the first time, and the yield of the glabridin can reach 60% or above.

The invention is suitable for the technical field of gene engineering, and provides a soybean MYB transcription factor gene and application thereof in increasing the content of isoflavone. The soybean MYB transcription factor gene is GmMYB3a, and the nucleotide sequence of the soybean MYB transcription factor gene is as shown in SEQ ID NO. 1. According to the invention, a pTF101-GmMYB3a overexpression vector and an RNAi-GmMYB3a interference vector are successfully constructed, and the constructed vectors are transferred into a receptor soybean variety Williams 82. Experimental results show that the soybean strain with overexpression of the GmMYB3a gene shows higher isoflavone content, while the soybean strain with interference of the GmMYB3a gene shows lower isoflavone content, and the positive regulation effect of the GmMYB3a in the isoflavone biosynthesis process is proved. The invention not only provides an innovative molecular breeding strategy for cultivating soybean strains with high isoflavone content, but also has remarkable application potential and market prospect.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention provides a traditional Chinese medicine composition for treating pancreatic fibrosis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition disclosed by the invention takes isoflavone as a main component, and the isoflavone not only can reverse the fibrosis degree of pancreatic fibrosis cells by retarding the proliferation and migration ability of fibrosis cells, influencing the cell activity and reducing the generation of various fibrosis proteins, but also can inhibit the illness state of mice with chronic pancreatic fibrosis; the disease progression is slow or blocked. According to the traditional Chinese medicine composition, the pancreatic fibrosis process is effectively reversed through retardation of isoflavone on pancreatic stellate cell fibrosis induced by TGF-beta1, the inhibition effect of the traditional Chinese medicine composition on pancreatic fibrosis can be improved in combination with other traditional Chinese medicine ingredients, and the ingredients are safe.

The invention provides a dihydroisoflavone compound, a preparation method and application thereof, a derivative of the dihydroisoflavone compound, application of the derivative and a pharmaceutical composition, and relates to the technical field of medicines. The dihydroisoflavone compound and the derivative thereof provided by the invention have a remarkable inhibition effect on triple-negative breast cancer cells, and the half inhibition concentration value of the dihydroisoflavone compound on the triple-negative breast cancer cells is 0.86 mu M and is equivalent to that of a positive drug paclitaxel; a new choice, a new thought and a technical support are provided for developing the medicine for treating the triple negative breast cancer, and the medicine has important clinical application value.

The invention relates to a genetically modified licorice stem cell and a preparation method thereof, and relates to the technical field of genetic engineering, and the licorice stem cell is modified by introducing a gene coding dimethyl allyl transferase (PcM4DT) or an active fragment thereof and a gene coding isoflavone synthase (IFS) or an active fragment thereof into the licorice stem cell. The content of secondary metabolites in the genetically modified liquorice stem cells is remarkably increased compared with that of unmodified liquorice stem cells, the increased secondary metabolites meet the industrial production requirement and can be directly used for high-end skin care products and medical products, and the liquorice resource value is expanded.

The invention provides a donkey-hide gelatin solid beverage and a preparation method thereof, the preparation method comprises the following steps: adding 8 times by mass of water into rhizoma polygonati, Chinese wolfberry, astragalus membranaceus, codonopsis pilosula, red dates, angelica sinensis and orange peel, soaking for 0.5 h, extracting for 2 h for the first time, adding 6 times by mass of water for extracting for 1.5 h for the second time, combining filtrates, and concentrating until the relative density is 1.23 g / cm < 3 > to obtain a water extraction concentrated solution; mixing the water extraction concentrated solution with maltodextrin, and drying to obtain an extract; and adding auxiliary materials into the extract and the donkey-hide gelatin, and molding to obtain the donkey-hide gelatin solid beverage. In the extraction step, proper soaking time, water adding amount and extraction time are combined, so that the ferulic acid content, the total polysaccharide content, the hesperidin content and the calycosin-7-glucoside content in the concentrated solution are all higher. And on the basis, subsequent drying and forming are carried out, so that the final donkey-hide gelatin solid beverage also has relatively high content of the components, and the content in each batch of products is stable.

The invention relates to a preparation method of a silymarin and pueraria isoflavone composite reagent. The preparation method comprises the following steps: S1, extracting silybin; s2, soaking the roots and stems of the kudzuvine roots; s3, extracting a radix puerariae isoflavone stock solution; s4, concentrating the radix puerariae isoflavone stock solution; s5, extracting a pueraria isoflavone concentrated solution; s6, mixing and granulating; and S7, tabletting and coating to obtain the flaky silymarin and pueraria isoflavone composite reagent. According to the prepared silymarin and pueraria isoflavone composite reagent, the silymarin dry powder and the pueraria isoflavone extract serve as core components, the silymarin dry powder can enhance liver detoxification and repair liver cells, the pueraria isoflavone extract can accelerate alcohol metabolism, and the silymarin dry powder and the pueraria isoflavone extract have a remarkable improvement effect on fatty liver, alcoholic liver and the like under the synergistic effect.

The invention provides a method for preparing chickpea tempeh by fermenting chickpeas. The method comprises the following steps: sprouting, introducing lactobacillus plantarum to carry out first fermentation, introducing rhizopus oryzae to carry out second fermentation, and the like. According to the method provided by the embodiment of the invention, the chickpea tempeh is endowed with richer flavor and better taste while functional components such as protein and isoflavone of the chickpea are improved, so that the chickpea tempeh has more excellent sensory quality and higher nutritional value.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention discloses a processing method of radix astragali stir-fried with honey and bran. The method comprises the following steps: refining honey, preparing wheat bran, preparing honey and bran and preparing radix astragali stir-fried with honey and bran. Through the optimized processing technology designed by the invention, the quality of radix astragali processed products is remarkably improved, and chemical components contained in the radix astragali processed products are reserved to the maximum extent, so that the safety, effectiveness and controllability of clinical medication of the radix astragali processed products are ensured. According to the radix astragali fried with honey and bran, the beany flavor is obviously weakened, so that the taste is improved, and the total content of the symbolic component astragaloside of the radix astragali and the five flavonoid components calycosin-7-glucoside, the mediodarin, the formononin, the formononetin and the calycosin-7-glucoside of the radix astragali is increased compared with that of the radix astragali. The invention revives the processing technology of honey-bran-fried astragalus membranaceus which is endangered to be lost, protects and excavates the special processing method of genuine medicinal materials in Gansu province, and can ensure that the special traditional Chinese medicine processing variety of honey-bran-fried astragalus membranaceus can be propagated in generations.

The invention relates to the technical field of natural medicines, in particular to a preparation method of active ingredients in fructus psoraleae and application of the active ingredients in anti-inflammatory products, firstly, a fructus psoraleae reflux extract is properly enriched by using an extraction method, then selective separation is performed by using different separation modes of HSCCC, and the active ingredients in the fructus psoraleae are obtained. And 10 monomeric compounds are separated by combining a prepared liquid phase. Through cell experiments, four compounds of psoralen, neopsoralen isoflavone, psoralen flavanone and psoralen flavanone methyl ether, which can well inhibit NO release, are screened out; the four monomeric compounds can reduce secretion of proinflammatory factors by weakening mRNA transcriptional ability and weaken over-phosphorylation expression of pathway protein, so that an anti-inflammatory effect is achieved; through verification, the fructus psoraleae flavanone and the fructus psoraleae flavanone methyl ether can play an anti-inflammatory role through MAPK and NF-kappa B signal channels.

The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.

The present invention discloses a method for separating 6-formylindomyrtone A from a high isoflavone mixture, and the steps are as follows: 1) Using the ophiopogon japonicus high isoflavone mixture as a raw material, dissolving the raw material in a mixed solution composed of ethyl acetate and a hydrophobic organic solvent to obtain a raw material solution; 2) Using a binary mixed solvent composed of an ionic liquid and water as an extractant and using the same solvent as the solvent of the raw material solution as a detergent, performing fractional extraction on the raw material solution, and collecting the raffinate; 3) Subjecting the raffinate to vacuum concentration, washing with water and drying treatment to obtain 6-formylindomyrtone A. The present invention uses an ionic liquid or a binary mixed solvent composed of an ionic liquid and a medium-polarity to polar organic solvent as an extractant, has a high selective separation ability for 6-formylindomyrtone A, and the purity and yield of the separated 6-formylindomyrtone A can both reach more than 90%.

The invention discloses a construction method and application of a specific chromatogram of a Shanhaidan pharmaceutical preparation, and belongs to the technical field of traditional Chinese medicine detection. The method comprises the following steps: preparing a test solution by using a plurality of different batches of Shanhaidan medicinal preparations in combination with high performance liquid chromatography and mass spectrometry technologies; the preparation method comprises the following steps: preparing a single reference substance solution from uridine, tanshinol, daidzin, p-hydroxybenzoic acid, puerarin, caffeic acid, 3 '-hydroxy puerarin, physcion, calycosin-7-O-beta-D-glucoside, stilbene glucoside, rosmarinic acid, alkannic acid, formononin, salvianolic acid B, daidzein and calycosin-7-one; and respectively carrying out high performance liquid chromatography analysis on the test solution and the reference solution to obtain the characteristic chromatogram of the Shanhaidan pharmaceutical preparation. The method is simple and convenient to operate, high in precision, good in reproducibility and accurate and reliable in result. And at least 16 components can be identified at one time under the same chromatographic condition, so that the detection efficiency is remarkably improved, the time and the cost are saved, and the quality of the Shanhaidan pharmaceutical preparation is comprehensively and scientifically evaluated.

The present invention relates to a functional coffee substitute composition. The coffee substitute food composition comprising a fermented dandelion root extract, a fermented Acanthopanax seed extract, and a fermented soybean extract of the present invention possesses sensory characteristics similar to coffee, and through fermentation, the content of major plant functional components such as isoflavone aglycone, total polyphenols, flavonoids, and saponins increases, L-carnitine is newly synthesized, and since it is a novel coffee substitute food material having antioxidant effects and anti-diabetic activity, it can be utilized as a caffeine-reducing beverage, a beverage that promotes body fat metabolism, an antioxidant-enhanced beverage, and a health functional food.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention discloses an application of polygonatum cyrtonema cyclase PcAS1 in promoting synthesis of hematoxylin A. The cyclase PcAS1 is derived from polygonatum cyrtonema, a high isoflavone compound hematoxylin A is synthesized in a plant body, and according to the application, a recombinant plant expression vector for expressing a PcAS1 gene is constructed and transformed into the plant to realize synthesis of the hematoxylin A. Based on multiple omics data such as whole genome, re-sequencing and transcriptome of polygonatum cyrtonema, key cyclase PcAS1 closely related to homoisoflavone biosynthesis is systematically mined. Through a tobacco transient expression system and a polygonatum cyrtonema hairy root transformation system, it is proved for the first time that cyclase PcAS1 can significantly promote synthesis of high isoflavone hematoxylon A, and biosynthesis of hematoxylon A is achieved in polygonatum cyrtonema for the first time.

Aspects of disclosure relate to a composition and methodology for treating menopause symptoms utilizing a cannabinoid-based nanoplatform composition. The composition includes phytocannabinoids like CBD, CBG, and CBN, alongside isoflavones and polyphenols, all integrated into nanoplatform designed for enhanced bioavailability and controlled release. The formulation is designed to alleviate key menopause-related issues, including vasomotor symptoms (VMS), genitourinary syndrome of menopause (GSM), bone loss, mood disturbances, and sleep disorders, while addressing cardiovascular disease (CVD) risks. The composition combines cannabinoids, such as CBD, with isoflavones and polyphenols, and is incorporated into nanoplatforms for enhanced delivery and efficacy. Additionally, the disclosure includes a kit comprising oral capsules, intravaginal ovules, and instructions for use. The capsules contain a blend of cannabinoids, flavonoids, and polyphenols, while the ovules are composed of CBD and cocoa butter. The kit provides a comprehensive solution for managing menopause symptoms and mitigating cardiovascular risks.

The invention discloses a preparation method and application of 7-hydroxyisoflavone acetylferulate, relating to the technical field of feed additives. The preparation method comprises the following steps: adding acetylferulic acid, 7-hydroxyisoflavone, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N,N-dimethyl-4-pyridinamine to a reactor; using dichloromethane as a solvent; stirring at room temperature for 5 hours; adding water after the reaction is complete; and then extracting with dichloromethane; and concentrating and crystallizing the product to obtain a target product. The product prepared by this method has good fat solubility and stability, and can be added as an additive to livestock, poultry, and aquatic animal feed to significantly improve the growth performance, antioxidant capacity, immunity, and intestinal digestion capacity of the animal.

The invention discloses an InDel molecular marker related to soybean grain weight, fat content and isoflavone content and application, and relates to the technical field of molecular detection. The nucleotide sequence of the InDel molecular marker is as shown in SEQ ID NO. 4. Whole genome association analysis finds that insertion / deletion variation of 16bp and 11bp exists in a specific region of a No.13 chromosome of soybean, and an InDel molecular marker related to soybean grain weight, fat content and isoflavone content is provided based on the insertion / deletion variation of 16bp and 11bp. By utilizing the InDel molecular marker, the genotype of a target site can be rapidly and accurately detected, and soybean materials with different genetic backgrounds can be distinguished, so that soybean varieties with excellent grain weight, fat and isoflavone content characters can be effectively screened in an auxiliary manner. According to the method, the breeding selection accuracy and efficiency are remarkably improved, and a powerful tool is provided for accelerating the soybean genetic improvement process.

The invention discloses a BRS-3 antagonist and application thereof in treating and / or preventing myocardial cell injury related diseases, and belongs to the field of biological medicine. It is found for the first time that licoflavone A and saikoside B2 can antagonize calcium ion (Ca < 2 + >) influx induced by a BRS-3 specific agonist MK5046 in a dose-dependent mode, and it is revealed that licoflavone A and saikoside B2 can serve as BRS-3 receptor antagonists to play a role. According to the application, an oxidative stress environment is simulated through hydrogen peroxide to explore the influence of licoflavone A of a BRS-3 receptor on myocardial cell activity, it is found for the first time that licoflavone A and bantag-1 can relieve myocardial cell injury caused by H2O2 by antagonizing BRS-3, it is revealed that the BRS-3 receptor possibly becomes a treatment target of myocardial injury caused by oxidative stress, and the application has a wide application prospect. The antagonist can be used for preparing medicines or health-care foods for treating or preventing myocardial cell injury related diseases.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

This utility model relates to a germination device for enriching soybean isoflavones, comprising a box body, a perforated tray disposed within the box body, and an ultraviolet lamp assembly positioned above the perforated tray. The ultraviolet lamp assembly emits ultraviolet light of at least UV-B wavelength. A lifting assembly is provided between the ultraviolet lamp assembly and the box body, and / or between the perforated tray and the box body. A spray assembly and a circulating air duct assembly are provided within the box body. An air duct control assembly, connected to the circulating air duct assembly, is located outside the box body and is used to regulate the temperature and humidity within the box body. This utility model employs a closed or semi-closed design, with a built-in ultraviolet lamp assembly emitting UV-B wavelength ultraviolet light. The ultraviolet lamps can be used individually or in combination, facilitating the adjustment of UV-B irradiation intensity and its uniformity, meeting the ultraviolet irradiation intensity requirements of germinating soybeans at different growth stages, and increasing the isoflavone content in germinating soybeans.

The invention relates to the field of mineral flotation, in particular to a dihydroxythioisoflavone copper-arsenic separation inhibitor and a preparation method and application thereof. The preparation method of the copper-arsenic separation inhibitor comprises the following steps: adding an 8-beta-D-glucopyranosyl-4 ', 7-dihydroxyisoflavone organic compound into a solvent, then adding a Lawson reagent, raising the temperature, and reacting to obtain a reaction solution; and cooling, filtering, purifying and crystallizing the reaction liquid to obtain the dihydroxythioisoflavone copper-arsenic separation inhibitor. The dihydroxy thioisoflavone copper-arsenic separation inhibitor prepared by the invention can selectively carry out oxidation inhibition on arsenic-containing minerals such as arsenopyrite, arsenhedrite and akaidite, reduces the content of arsenic in copper concentrate, is simple to apply on site, does not need to change the process flow on site, only needs to adjust the dosage of the medicament and add the medicament according to the amount, is simple to operate, and is suitable for industrial production. The index is stable.