139 results about "Isoflavones" patented technology

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.

Aspects of disclosure relate to a composition and methodology for treating menopause symptoms utilizing a cannabinoid-based nanoplatform composition. The composition includes phytocannabinoids like CBD, CBG, and CBN, alongside isoflavones and polyphenols, all integrated into nanoplatform designed for enhanced bioavailability and controlled release. The formulation is designed to alleviate key menopause-related issues, including vasomotor symptoms (VMS), genitourinary syndrome of menopause (GSM), bone loss, mood disturbances, and sleep disorders, while addressing cardiovascular disease (CVD) risks. The composition combines cannabinoids, such as CBD, with isoflavones and polyphenols, and is incorporated into nanoplatforms for enhanced delivery and efficacy. Additionally, the disclosure includes a kit comprising oral capsules, intravaginal ovules, and instructions for use. The capsules contain a blend of cannabinoids, flavonoids, and polyphenols, while the ovules are composed of CBD and cocoa butter. The kit provides a comprehensive solution for managing menopause symptoms and mitigating cardiovascular risks.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

This utility model relates to a germination device for enriching soybean isoflavones, comprising a box body, a perforated tray disposed within the box body, and an ultraviolet lamp assembly positioned above the perforated tray. The ultraviolet lamp assembly emits ultraviolet light of at least UV-B wavelength. A lifting assembly is provided between the ultraviolet lamp assembly and the box body, and / or between the perforated tray and the box body. A spray assembly and a circulating air duct assembly are provided within the box body. An air duct control assembly, connected to the circulating air duct assembly, is located outside the box body and is used to regulate the temperature and humidity within the box body. This utility model employs a closed or semi-closed design, with a built-in ultraviolet lamp assembly emitting UV-B wavelength ultraviolet light. The ultraviolet lamps can be used individually or in combination, facilitating the adjustment of UV-B irradiation intensity and its uniformity, meeting the ultraviolet irradiation intensity requirements of germinating soybeans at different growth stages, and increasing the isoflavone content in germinating soybeans.

The application discloses a lactobacillus crispatus based on multiple targets to realize anti-aging effect and application of transformation of glycyrrhiza for efficiency enhancement, and belongs to the technical field of microorganisms and the technical field of medicine. The lactobacillus crispatus CCFM1362 provided in the application can ferment glycyrrhiza extract, can improve the content of glycyrrhizic acid, hemiglycyrrhizin isoflavone B, naringenin chalcone and other substances, can enhance the efficacy of glycyrrhiza extract, and can realize the effect of multiple targets for anti-aging, and the specific embodiments are as follows: (1) the content of beta-galactosidase in liver tissue is inhibited; (2) the level of aging-related secretory phenotype in liver tissue is reduced; (3) the Nrf2 related pathway is activated, and the level of oxidative stress is reduced; (4) the NF-kappa B related pathway is activated, and the level of chronic inflammation is reduced. Therefore, the lactobacillus crispatus CCFM1362 and the glycyrrhiza extract have great application prospect in the production of anti-aging products.

The present invention provides a protective film agent and a method for processing a workpiece, the protective film agent being capable of forming a protective film suitable for processing with a laser beam. The protective film agent is coated on a workpiece when the workpiece is processed, the protective film agent comprising: a water-soluble resin; a light absorber having a flavone structure, a flavonol structure, or an isoflavone structure; and a solvent that dissolves the resin and the light absorber.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention provides an astragalus membranaceus calycosin-7-methyltransferase protein AmOMT1, a coding gene, a primer group and application of the astragalus membranaceus calycosin-7-methyltransferase protein AmOMT1, and belongs to the technical field of biology. The amino acid sequence of the astragalus membranaceus calycosin-7-methyltransferase protein AmOMT1 is as shown in SEQ ID No. 1 (sequence identifier number 1). According to the present invention, based on the radix astragali seu hedysari genome and the second-generation transcriptome data, the transmethylase AmOMT1 having 4 'and 7-site methylation functions on 3', 4 ', 7-trihydroxy isoflavone simultaneously is found and identified by using the reverse genetics method, and the experiment results show that the AmOMT1 can catalyze the methylation of the 3', 4 ', 7-trihydroxy isoflavone to generate calycosin and 3', 4 '-dihydroxy-7-isoflavone, and the transmethylase AmOMT1 can catalyze the methylation of the 3', 4 ', 7-trihydroxy isoflavone can catalyze the methylation of the 3', 4 ', 7-trihydroxy isoflavone to generate the 3', 4 '-dihydroxy-7-isoflavone.

The application relates to application of C-methylated high isoflavone compounds in preparation of antitumor drugs and belongs to the technical field of drug application. In order to solve the problem that the existing antitumor activity is not much reported, the application of C-methylated high isoflavone compounds in preparation of antitumor drugs is provided, the C-methylated high isoflavone compounds are selected from compounds shown in the following formula 1 or formula 2: the C-methylated high isoflavone compounds or pharmaceutically acceptable salts thereof are used as active ingredients for preparation of antitumor drugs for preventing and / or treating adrenal pheochromocytoma (PC-12) and / or human brain astrocytoma (U-87MG). The C-methylated high isoflavone compounds have the advantages of high activity and high inhibition capacity, can be extracted from polygonatum sibiricum and have good drug safety.

This invention discloses an isoflavone compound, its preparation method, and its applications. The compounds are derived by directional esterification modification of all phenolic hydroxyl groups with allyloxycarbonyl chloride, o-chlorobenzoyl chloride, or cinnamoyl chloride, using 4',7-dihydroxyisoflavone or 4',5,7-trihydroxyisoflavone as the parent nucleus. The invention also provides a mild and efficient preparation method for these compounds and confirms their significant anti-inflammatory pharmacological activity. Pharmacological studies show that compounds B and E, in particular, exhibit excellent in vitro and in vivo anti-inflammatory activity, with compound E showing superior activity compared to the positive control drug dexamethasone. These compounds can be used to prepare drugs for treating inflammatory diseases such as pneumonia and colitis.

The invention belongs to the field of functional food processing, and provides a preparation process of functional soybean milk powder with a sleep improving effect, which adopts a five-step linkage design of germinated soybean enzymolysis, lactobacillus plantarum fermentation, in-situ calcium nucleation, isoelectric point adsorption, exopolysaccharide stabilization and spray drying. Layered coating of the fermentation system autogenous exopolysaccharide and the fermentation modified soybean protein on the organic acid calcium microcrystal core is realized through pH gradient dynamic regulation and control, and a core-protein middle layer-polysaccharide outer layer ternary in-situ composite microcapsule structure is constructed. The multi-target synergy that the gamma-aminobutyric acid is enriched to 150-300 mg / 100 g, the isoflavone aglycone rate reaches 70-85% and the microcapsule D50 is controlled to be 5-80 microns is achieved, the three technical problems that high-solid-content low-viscosity spraying and strong interaction conflict exists, the dry powder flowability and core-shell layer stability conflict exists, and a narrow pH window and amplification tolerance are opposite are solved, and the method has wide functional food application value.

The application discloses a composition for treating lumbar intervertebral disc herniation and repairing annulus fibrosus, relates to the technical field of traditional Chinese medicine compositions, and comprises the following raw materials: radix rehmanniae preparata, plastrum testudinis, fructus psoraleae, eucommia ulmoides oliver, walnut kernel, bee pollen, ligustilide, saikosaponin A, salvianolic acid B, ferulic acid, calycosin, stibene glycoside, prunol, loganin, hydroxysafflor yellow A and ligustilide. Experiments show that the composition can strengthen the repair of annulus fibrosus, has anti-inflammatory and analgesic effects, protects collagen, inhibits degeneration, can effectively treat lumbar intervertebral disc herniation and realize the structural repair of damaged annulus fibrosus, and provides a new technical scheme for the clinical treatment of lumbar intervertebral disc herniation.

The invention discloses a glycosyl transferase combined mutant and application thereof, and aims to solve the problems that the existing wild PlUGT43 and F196Y single-point mutants thereof have narrow substrate spectrums and cannot efficiently catalyze a plurality of isoflavone derivatives with large structural differences to perform C-glycosylation reaction. The glycosyl transferase combination mutant provided by the invention is a protein which simultaneously contains five mutated sites, namely T81S, N186S, L187A, F196Y and S310P, on the basis of a wild PlUGT43 amino acid sequence as shown in SEQ ID NO.3. The glycosyl transferase combination mutant provided by the invention is a protein which is obtained by mutating five sites, namely T81S, N186S, L187A, F196Y and S310P. Compared with a wild type and an F196Y single-site mutant, the combined mutant has the advantages that the catalytic activity on classical substrates such as daidzein is improved, and efficient C-glycosylation on various substrates such as soybean dihydroaglycone, equol and resveratrol which are difficult to catalyze or extremely low in catalytic efficiency is also realized; the application potential of the glycosyl transferase in the aspect of synthesis of diversified natural products is improved.

A cosmetic lip care composition for improving the appearance of the lips, characterized in that it comprises at least one plant-based isoflavone component and a cosmetically compatible carrier base, wherein the composition is intended for topical application to the lips.

A small molecule composition, comprising licochalcone A, licochalcone C, licochalcone D, licochalcone E, echinatin, isoliquiritigenin, formononetin, liquiritigenin, licoflavone C, glabrol, licoisoflavone A and licoisoflavone B, and the use thereof in preparing anticancer or antitumor drugs, anti-ferroptosis drugs, or drugs for sensitization of anticancer or antitumor drugs. The small molecule composition can also be used in combination with other antitumor drugs.

The application discloses application of a Chinese medicine pair effective component composition of Astragalus membranaceus and Curcuma zedoary in preparation of anti-tumor metastasis drugs. The composition is composed of calycosin-7-glucoside and curcumin. The composition can effectively inhibit transendothelial invasion and vasculogenic mimicry generation of tumor cells at a non-toxic dose, and has the advantages of high efficiency and low toxicity in treating tumor metastasis. The important point of the application is that the preferred composition can significantly reduce the exosome LAP-TGF-beta1 protein loading level, thereby greatly inhibiting the tumor metastasis process caused by the exosome-mediated TGF-beta signal pathway. The finding provides a new perspective for the development of drugs for targeting exosome LAP-TGF-beta1 protein loading to resist tumor metastasis, and overcomes the defects of unclear effective components, uncontrollable proportioning and unclear treatment effect of traditional Chinese medicine compatibility. Therefore, the Chinese medicine effective component composition has application prospects in preparation of anti-tumor metastasis drugs.

The invention provides an anti-alcohol functional water based on liver enzyme activation, and relates to the technical field of functional water. The hangover alleviating functional water based on liver enzyme activation is prepared from radix puerariae isoflavone aglycone, globe artichoke water-soluble polysaccharide, emblic leafflower fruit procyanidine oligomer, an electrolyte combination, a pH buffer system, hovenia dulcis thunb, fructus gardeniae, beta-nicotinamide mononucleotide, hydrogen-rich water, vitamin C, vitamin E and curcumin, and the electrolyte combination is prepared from sodium chloride, potassium chloride and sodium bicarbonate. Alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver can be activated through the radix puerariae isoflavone aglycone, the globe artichoke water-soluble polysaccharide, the emblic leafflower fruit proanthocyanidin oligomer and the like, so that decomposition and metabolism of alcohol are accelerated, the retention time of the alcohol in the body can be remarkably shortened by activating the enzymes and dispelling drunkenness, and the symptoms of drunkenness and hangover are reduced.

The invention relates to the field of natural product pharmacy, in particular to an acetylcholin esterase inhibitor sourced from a natural product and application of the acetylcholin esterase inhibitor. Although a chemical synthetic acetylcholin esterase inhibitor used in clinical treatment at present achieves a certain curative effect, the chemical synthetic acetylcholin esterase inhibitor still has the problems of side effects, drug resistance and the like. Therefore, the invention provides the acetylcholin esterase inhibitor, the inhibitor is a single-drug or double-drug composition derived from natural products, the single drug is grifolin isoflavone (TI) or isoeugenol acetate (IA), and the double drug is prepared from TI and IA in proportion. The acetylcholin esterase inhibitor disclosed by the invention has good AChE inhibitory activity, anti-Abeta aggregation effect, antioxidant activity and good neuroprotection effect, and the TI and / or IA can be used as a novel acetylcholin esterase inhibitor to be applied to the development and use of medicines for clinically treating and / or preventing related diseases such as Alzheimer's disease.

The application provides a astragalus membranaceus calyx heterophylla isoflavone methyltransferase protein AmOMT1, a coding gene, a primer set and application thereof, and belongs to the technical field of biotechnology.The amino acid sequence of the astragalus membranaceus calyx heterophylla isoflavone methyltransferase protein AmOMT1 is shown as SEQ ID No.1.Based on astragalus membranaceus genomic and second-generation transcriptomic data, a methyltransferase AmOMT1 with 4' and 7 position methylation functions for 3', 4', 7-trihydroxyisoflavone is found and identified by a reverse genetics method.Experiments show that AmOMT1 can catalyze the methylation of 3', 4', 7-trihydroxyisoflavone to generate calyx heterophylla isoflavone and 3', 4'-dihydroxy-7-methoxyisoflavone.

The application relates to the technical field of fluorescence detection, in particular to a method for detecting the composition of a lithium metal negative electrode, wherein the lithium metal negative electrode is a lithium metal negative electrode after charge-discharge cycle in a lithium battery, and the method comprises the following steps: adopting an isoflavone compound as a fluorescent probe to detect the composition on the surface of the lithium metal negative electrode. Adopting the isoflavone compound as the probe can realize visual observation and quantitative detection of deposited lithium, lithium dendrites, by-products, dead lithium and a solid electrolyte interface film on the surface of the lithium metal negative electrode.

To provide a tablet containing isoflavones that achieves both hardness and compressibility. [Solution] The tablets of the present invention contain (A) isoflavones, (B) excipients, and (C) lubricants. It is preferable that (A) isoflavones contain daidzein and genistein. It is also preferable that the mass ratio of daidzein to genistein is genistein:daidzein = 1:0.5 to 4.5. Furthermore, it is also preferable that the tablets contain (D) a fluidizing agent.

The invention relates to the technical field of food biology, in particular to a high-activity fermented astragalus mongholicus and red date compound for improving myocardial function, which is prepared by taking Xinjiang Qiang red dates and astragalus mongholicus as main raw materials and matching cellulase, pectinase, beta-glucanase and hydroxypropyl-beta-cyclodextrin. In the preparation process, gradient freezing-microwave pretreatment and ternary composite enzymolysis synergistic wall breaking are adopted, quaternary strains of bifidobacterium longum, bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum are compounded, adenosine concentration response type staged solid-state fermentation is performed, and microcapsule embedding stabilizing treatment is performed to obtain the product. The content of cAMP in the product is greater than or equal to 13.0 mu g / g, and the content of calycosin-7-glucoside in astragalus membranaceus is greater than or equal to 1.9 mg / g. The compound can be used for preparing myocardial ischemia pretreatment health-care food and a pharmaceutical composition for adjuvant therapy in the myocardial infarction recovery period, and the dosage forms comprise chewable tablets, oral liquid, capsules and the like.

The present application belongs to the field of Chinese medicine preparation, and relates to a quality detection method of a Chinese medicine compound composition for benefiting kidney and dispelling wind. The Chinese medicine compound composition for benefiting kidney and dispelling wind comprises the following components in weight fractions: 9-30 parts of raw astragalus, 6-12 parts of wine holly fruit, 3-6 parts of scorpion, 5-10 parts of fried cantharis, 9-30 parts of prince salvia, 6-12 parts of fried atractylodes, 6-30 parts of pyrrosia, 3-10 parts of safflower, 3-10 parts of chuanxiong, 15-30 parts of white flower snake tongue, 15-30 parts of poria bark, and 9-30 parts of plantago seed. The quality detection method of the Chinese medicine compound composition adopts thin layer chromatography to identify astragalus and wine holly fruit, and further improves the comprehensiveness and reliability of the quality monitoring of the Chinese medicine compound composition by determining the content of calycosin-7-glucoside, and more perfectly characterizes the effective active components and quality of the medicine, which is beneficial to the comprehensive monitoring of the active components and further guarantees the stability, consistency, controllability, safety and effectiveness of the Chinese medicine compound composition.

The invention relates to the technical field of agricultural biology. The invention provides application of exogenous treatment in promotion of plant germination and enrichment of isoflavone content. The exogenous treatment is hydrogen sulfide donor treatment. Preferably, the plant is soybean, and the hydrogen sulfide donor treatment is applied to at least one of the following (b1)-(b28). Preferably, the hydrogen sulfide donor is NaHS (sodium hydrogen sulfide). The invention further provides a method for promoting plant germination. The invention also provides a special culture solution for implementing the method. The invention further provides a method for promoting enrichment of isoflavone in plants. The invention also provides the soybean sprouts produced by the method. According to the method, the NaSH serving as a hydrogen sulfide donor is adopted for treatment, so that soybean germination can be promoted, the extension of the soybean sprouts is promoted, and the fresh weight of the soybean sprouts is also promoted; meanwhile, the enrichment of isoflavone content can be promoted, and a basis is provided for producing functional bean sprout products with higher nutritional value.

The present invention relates to a method for preparing extracellular vesicles of Lactobacillus cells with increased aglycone isoflavone content, and a composition for preventing hair loss using same. The Lactobacillus of the present invention enables the effective extraction of aglycone isoflavones contained in legumes through a fermentation process, and the extracellular vesicles secreted from the Lactobacillus exhibit anti-inflammatory and immunomodulatory properties and can be expected to have the effects of promoting scalp health and preventing hair loss. Owing to their small size and excellent intercellular delivery capability, the extracellular vesicles can act effectively in deep layers of the skin and scalp. In addition, the extracellular vesicles have high biocompatibility and fewer side effects, and can be used more safely than conventional chemical compositions.

The invention discloses application of licoflavone A in resisting viral hepatitis B, and belongs to the technical field of medicine application. The licoflavone A is used for treating viral hepatitis B. The licoflavone A used in the invention shows good safety and tolerance in HepG2.2. 15 and HepG2.A64 cells, can play a role in resisting HBV under the condition of no obvious cytotoxicity, and has significant advantages compared with interferon drugs in the prior art with long treatment course, large side effect and poor tolerance.