247 results about "Isoflavones" patented technology

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention provides a dihydroisoflavone compound, a preparation method and application thereof, a derivative of the dihydroisoflavone compound, application of the derivative and a pharmaceutical composition, and relates to the technical field of medicines. The dihydroisoflavone compound and the derivative thereof provided by the invention have a remarkable inhibition effect on triple-negative breast cancer cells, and the half inhibition concentration value of the dihydroisoflavone compound on the triple-negative breast cancer cells is 0.86 mu M and is equivalent to that of a positive drug paclitaxel; a new choice, a new thought and a technical support are provided for developing the medicine for treating the triple negative breast cancer, and the medicine has important clinical application value.

The invention relates to a genetically modified licorice stem cell and a preparation method thereof, and relates to the technical field of genetic engineering, and the licorice stem cell is modified by introducing a gene coding dimethyl allyl transferase (PcM4DT) or an active fragment thereof and a gene coding isoflavone synthase (IFS) or an active fragment thereof into the licorice stem cell. The content of secondary metabolites in the genetically modified liquorice stem cells is remarkably increased compared with that of unmodified liquorice stem cells, the increased secondary metabolites meet the industrial production requirement and can be directly used for high-end skin care products and medical products, and the liquorice resource value is expanded.

The invention relates to a preparation method of a silymarin and pueraria isoflavone composite reagent. The preparation method comprises the following steps: S1, extracting silybin; s2, soaking the roots and stems of the kudzuvine roots; s3, extracting a radix puerariae isoflavone stock solution; s4, concentrating the radix puerariae isoflavone stock solution; s5, extracting a pueraria isoflavone concentrated solution; s6, mixing and granulating; and S7, tabletting and coating to obtain the flaky silymarin and pueraria isoflavone composite reagent. According to the prepared silymarin and pueraria isoflavone composite reagent, the silymarin dry powder and the pueraria isoflavone extract serve as core components, the silymarin dry powder can enhance liver detoxification and repair liver cells, the pueraria isoflavone extract can accelerate alcohol metabolism, and the silymarin dry powder and the pueraria isoflavone extract have a remarkable improvement effect on fatty liver, alcoholic liver and the like under the synergistic effect.

The invention provides a method for preparing chickpea tempeh by fermenting chickpeas. The method comprises the following steps: sprouting, introducing lactobacillus plantarum to carry out first fermentation, introducing rhizopus oryzae to carry out second fermentation, and the like. According to the method provided by the embodiment of the invention, the chickpea tempeh is endowed with richer flavor and better taste while functional components such as protein and isoflavone of the chickpea are improved, so that the chickpea tempeh has more excellent sensory quality and higher nutritional value.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to the technical field of natural medicines, in particular to a preparation method of active ingredients in fructus psoraleae and application of the active ingredients in anti-inflammatory products, firstly, a fructus psoraleae reflux extract is properly enriched by using an extraction method, then selective separation is performed by using different separation modes of HSCCC, and the active ingredients in the fructus psoraleae are obtained. And 10 monomeric compounds are separated by combining a prepared liquid phase. Through cell experiments, four compounds of psoralen, neopsoralen isoflavone, psoralen flavanone and psoralen flavanone methyl ether, which can well inhibit NO release, are screened out; the four monomeric compounds can reduce secretion of proinflammatory factors by weakening mRNA transcriptional ability and weaken over-phosphorylation expression of pathway protein, so that an anti-inflammatory effect is achieved; through verification, the fructus psoraleae flavanone and the fructus psoraleae flavanone methyl ether can play an anti-inflammatory role through MAPK and NF-kappa B signal channels.

The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.

The present invention discloses a method for separating 6-formylindomyrtone A from a high isoflavone mixture, and the steps are as follows: 1) Using the ophiopogon japonicus high isoflavone mixture as a raw material, dissolving the raw material in a mixed solution composed of ethyl acetate and a hydrophobic organic solvent to obtain a raw material solution; 2) Using a binary mixed solvent composed of an ionic liquid and water as an extractant and using the same solvent as the solvent of the raw material solution as a detergent, performing fractional extraction on the raw material solution, and collecting the raffinate; 3) Subjecting the raffinate to vacuum concentration, washing with water and drying treatment to obtain 6-formylindomyrtone A. The present invention uses an ionic liquid or a binary mixed solvent composed of an ionic liquid and a medium-polarity to polar organic solvent as an extractant, has a high selective separation ability for 6-formylindomyrtone A, and the purity and yield of the separated 6-formylindomyrtone A can both reach more than 90%.

The invention discloses a construction method and application of a specific chromatogram of a Shanhaidan pharmaceutical preparation, and belongs to the technical field of traditional Chinese medicine detection. The method comprises the following steps: preparing a test solution by using a plurality of different batches of Shanhaidan medicinal preparations in combination with high performance liquid chromatography and mass spectrometry technologies; the preparation method comprises the following steps: preparing a single reference substance solution from uridine, tanshinol, daidzin, p-hydroxybenzoic acid, puerarin, caffeic acid, 3 '-hydroxy puerarin, physcion, calycosin-7-O-beta-D-glucoside, stilbene glucoside, rosmarinic acid, alkannic acid, formononin, salvianolic acid B, daidzein and calycosin-7-one; and respectively carrying out high performance liquid chromatography analysis on the test solution and the reference solution to obtain the characteristic chromatogram of the Shanhaidan pharmaceutical preparation. The method is simple and convenient to operate, high in precision, good in reproducibility and accurate and reliable in result. And at least 16 components can be identified at one time under the same chromatographic condition, so that the detection efficiency is remarkably improved, the time and the cost are saved, and the quality of the Shanhaidan pharmaceutical preparation is comprehensively and scientifically evaluated.

The present invention relates to a functional coffee substitute composition. The coffee substitute food composition comprising a fermented dandelion root extract, a fermented Acanthopanax seed extract, and a fermented soybean extract of the present invention possesses sensory characteristics similar to coffee, and through fermentation, the content of major plant functional components such as isoflavone aglycone, total polyphenols, flavonoids, and saponins increases, L-carnitine is newly synthesized, and since it is a novel coffee substitute food material having antioxidant effects and anti-diabetic activity, it can be utilized as a caffeine-reducing beverage, a beverage that promotes body fat metabolism, an antioxidant-enhanced beverage, and a health functional food.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention discloses an application of polygonatum cyrtonema cyclase PcAS1 in promoting synthesis of hematoxylin A. The cyclase PcAS1 is derived from polygonatum cyrtonema, a high isoflavone compound hematoxylin A is synthesized in a plant body, and according to the application, a recombinant plant expression vector for expressing a PcAS1 gene is constructed and transformed into the plant to realize synthesis of the hematoxylin A. Based on multiple omics data such as whole genome, re-sequencing and transcriptome of polygonatum cyrtonema, key cyclase PcAS1 closely related to homoisoflavone biosynthesis is systematically mined. Through a tobacco transient expression system and a polygonatum cyrtonema hairy root transformation system, it is proved for the first time that cyclase PcAS1 can significantly promote synthesis of high isoflavone hematoxylon A, and biosynthesis of hematoxylon A is achieved in polygonatum cyrtonema for the first time.

Aspects of disclosure relate to a composition and methodology for treating menopause symptoms utilizing a cannabinoid-based nanoplatform composition. The composition includes phytocannabinoids like CBD, CBG, and CBN, alongside isoflavones and polyphenols, all integrated into nanoplatform designed for enhanced bioavailability and controlled release. The formulation is designed to alleviate key menopause-related issues, including vasomotor symptoms (VMS), genitourinary syndrome of menopause (GSM), bone loss, mood disturbances, and sleep disorders, while addressing cardiovascular disease (CVD) risks. The composition combines cannabinoids, such as CBD, with isoflavones and polyphenols, and is incorporated into nanoplatforms for enhanced delivery and efficacy. Additionally, the disclosure includes a kit comprising oral capsules, intravaginal ovules, and instructions for use. The capsules contain a blend of cannabinoids, flavonoids, and polyphenols, while the ovules are composed of CBD and cocoa butter. The kit provides a comprehensive solution for managing menopause symptoms and mitigating cardiovascular risks.

The invention discloses a preparation method and application of 7-hydroxyisoflavone acetylferulate, relating to the technical field of feed additives. The preparation method comprises the following steps: adding acetylferulic acid, 7-hydroxyisoflavone, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N,N-dimethyl-4-pyridinamine to a reactor; using dichloromethane as a solvent; stirring at room temperature for 5 hours; adding water after the reaction is complete; and then extracting with dichloromethane; and concentrating and crystallizing the product to obtain a target product. The product prepared by this method has good fat solubility and stability, and can be added as an additive to livestock, poultry, and aquatic animal feed to significantly improve the growth performance, antioxidant capacity, immunity, and intestinal digestion capacity of the animal.

The invention discloses an InDel molecular marker related to soybean grain weight, fat content and isoflavone content and application, and relates to the technical field of molecular detection. The nucleotide sequence of the InDel molecular marker is as shown in SEQ ID NO. 4. Whole genome association analysis finds that insertion / deletion variation of 16bp and 11bp exists in a specific region of a No.13 chromosome of soybean, and an InDel molecular marker related to soybean grain weight, fat content and isoflavone content is provided based on the insertion / deletion variation of 16bp and 11bp. By utilizing the InDel molecular marker, the genotype of a target site can be rapidly and accurately detected, and soybean materials with different genetic backgrounds can be distinguished, so that soybean varieties with excellent grain weight, fat and isoflavone content characters can be effectively screened in an auxiliary manner. According to the method, the breeding selection accuracy and efficiency are remarkably improved, and a powerful tool is provided for accelerating the soybean genetic improvement process.

The invention discloses a BRS-3 antagonist and application thereof in treating and / or preventing myocardial cell injury related diseases, and belongs to the field of biological medicine. It is found for the first time that licoflavone A and saikoside B2 can antagonize calcium ion (Ca < 2 + >) influx induced by a BRS-3 specific agonist MK5046 in a dose-dependent mode, and it is revealed that licoflavone A and saikoside B2 can serve as BRS-3 receptor antagonists to play a role. According to the application, an oxidative stress environment is simulated through hydrogen peroxide to explore the influence of licoflavone A of a BRS-3 receptor on myocardial cell activity, it is found for the first time that licoflavone A and bantag-1 can relieve myocardial cell injury caused by H2O2 by antagonizing BRS-3, it is revealed that the BRS-3 receptor possibly becomes a treatment target of myocardial injury caused by oxidative stress, and the application has a wide application prospect. The antagonist can be used for preparing medicines or health-care foods for treating or preventing myocardial cell injury related diseases.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

This utility model relates to a germination device for enriching soybean isoflavones, comprising a box body, a perforated tray disposed within the box body, and an ultraviolet lamp assembly positioned above the perforated tray. The ultraviolet lamp assembly emits ultraviolet light of at least UV-B wavelength. A lifting assembly is provided between the ultraviolet lamp assembly and the box body, and / or between the perforated tray and the box body. A spray assembly and a circulating air duct assembly are provided within the box body. An air duct control assembly, connected to the circulating air duct assembly, is located outside the box body and is used to regulate the temperature and humidity within the box body. This utility model employs a closed or semi-closed design, with a built-in ultraviolet lamp assembly emitting UV-B wavelength ultraviolet light. The ultraviolet lamps can be used individually or in combination, facilitating the adjustment of UV-B irradiation intensity and its uniformity, meeting the ultraviolet irradiation intensity requirements of germinating soybeans at different growth stages, and increasing the isoflavone content in germinating soybeans.

The invention relates to the field of mineral flotation, in particular to a dihydroxythioisoflavone copper-arsenic separation inhibitor and a preparation method and application thereof. The preparation method of the copper-arsenic separation inhibitor comprises the following steps: adding an 8-beta-D-glucopyranosyl-4 ', 7-dihydroxyisoflavone organic compound into a solvent, then adding a Lawson reagent, raising the temperature, and reacting to obtain a reaction solution; and cooling, filtering, purifying and crystallizing the reaction liquid to obtain the dihydroxythioisoflavone copper-arsenic separation inhibitor. The dihydroxy thioisoflavone copper-arsenic separation inhibitor prepared by the invention can selectively carry out oxidation inhibition on arsenic-containing minerals such as arsenopyrite, arsenhedrite and akaidite, reduces the content of arsenic in copper concentrate, is simple to apply on site, does not need to change the process flow on site, only needs to adjust the dosage of the medicament and add the medicament according to the amount, is simple to operate, and is suitable for industrial production. The index is stable.

The invention discloses a cell cellulose composition and application thereof in skin care products, and belongs to the technical field of cellulose preparation. According to the composition, bacterial cellulose, modified amphiphilic grafted cellulose micelles and cellulose nanocrystals are taken as cores, an isoflavone active group is grafted to a cellulose chain through an RAFT polymerization technology, an amphiphilic carrier capable of loading an oil-soluble active matter is formed, grape seed oil, resveratrol and other active ingredients can be loaded, slow release and controlled release are achieved, and the composition has a good application prospect. A bacterial cellulose three-dimensional network and nanocrystals form a protective film on the skin surface, hyaluronic acid can enhance cuticle lipid arrangement and accelerate skin barrier function reconstruction, an isoflavone grafted structure provides ultraviolet protection and anti-inflammatory pathway regulation and control, inflammatory factor release is remarkably inhibited, and the anti-inflammatory effect is achieved. A cellulose nanocrystal and bacterial cellulose composite network is provided to improve the stability of the emulsion, the problem that a traditional product is prone to layering is solved, and the emulsion has the effects of physical barrier, long-acting moisturizing and repairing and is suitable for anti-allergy repairing type skin care products such as essence and masks.

The application discloses a lactobacillus crispatus based on multiple targets to realize anti-aging effect and application of transformation of glycyrrhiza for efficiency enhancement, and belongs to the technical field of microorganisms and the technical field of medicine. The lactobacillus crispatus CCFM1362 provided in the application can ferment glycyrrhiza extract, can improve the content of glycyrrhizic acid, hemiglycyrrhizin isoflavone B, naringenin chalcone and other substances, can enhance the efficacy of glycyrrhiza extract, and can realize the effect of multiple targets for anti-aging, and the specific embodiments are as follows: (1) the content of beta-galactosidase in liver tissue is inhibited; (2) the level of aging-related secretory phenotype in liver tissue is reduced; (3) the Nrf2 related pathway is activated, and the level of oxidative stress is reduced; (4) the NF-kappa B related pathway is activated, and the level of chronic inflammation is reduced. Therefore, the lactobacillus crispatus CCFM1362 and the glycyrrhiza extract have great application prospect in the production of anti-aging products.

The present invention provides a protective film agent and a method for processing a workpiece, the protective film agent being capable of forming a protective film suitable for processing with a laser beam. The protective film agent is coated on a workpiece when the workpiece is processed, the protective film agent comprising: a water-soluble resin; a light absorber having a flavone structure, a flavonol structure, or an isoflavone structure; and a solvent that dissolves the resin and the light absorber.

The invention provides an establishment method of a specific chromatogram of an angelica sinensis blood-enriching oral liquid as well as the specific chromatogram and application of the angelica sinensis blood-enriching oral liquid, and the establishment method is characterized by comprising the following steps: taking ferulic acid, calycosin, calycosin glucoside, formononin and ligustilide reference substances, and adding a methanol solution to prepare a reference substance solution; adding a methanol solution into the angelica sinensis blood-enriching oral liquid, filtering, and taking filtrate to prepare a test solution; a reference substance solution and a test solution are taken respectively, a high performance liquid chromatograph is adopted for detection, a mobile phase A is an acetonitrile solution, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration being 0.05%-0.2%, elution is conducted according to an elution procedure, and the specific chromatogram of the angelica sinensis blood replenishing oral liquid is established through chromatographic analysis. According to the method, the peak corresponding to the calycosin-7-glucoside peak is determined as the S peak, and the specific chromatogram of the angelica sinensis blood-enriching oral liquid is evaluated by adopting the relative retention time, so that the quality of the angelica sinensis blood-enriching oral liquid can be detected and evaluated comprehensively, objectively and accurately.

This disclosure relates to a method for quantitatively detecting active ingredients in Ophiopogon japonicus and its application. The method includes: S1, placing the Ophiopogon japonicus to be tested in an extract, shaking, sonicating, centrifuging, and then mixing with an internal standard to obtain a test solution; S2, using liquid chromatography-mass spectrometry to detect the content of active ingredients in the test solution; the active ingredients include flavonoids and saponins. This method can simultaneously and rapidly quantitatively analyze multiple active substances in Ophiopogon japonicus, including steroidal saponins with different physicochemical properties, high levels of isoflavones, and ketones, which is of great significance for evaluating the quality level of Ophiopogon japonicus.

To improve the equol intake of an individual user.SOLUTION: A recommended amount output system includes one or more computers that perform the followings: calculating a user's equol deficiency amount using a predicted value of the user's equol production amount and a predetermined target amount; calculating a recommended amount of equol or isoflavone that the user should ingest based on the calculated equol deficiency amount and an index value representing the degree of the user's equol production ability; and outputting the calculated recommended amount.SELECTED DRAWING: Figure 3

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).