178 results about "Isoflavones" patented technology

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention relates to a preparation method of a silymarin and pueraria isoflavone composite reagent. The preparation method comprises the following steps: S1, extracting silybin; s2, soaking the roots and stems of the kudzuvine roots; s3, extracting a radix puerariae isoflavone stock solution; s4, concentrating the radix puerariae isoflavone stock solution; s5, extracting a pueraria isoflavone concentrated solution; s6, mixing and granulating; and S7, tabletting and coating to obtain the flaky silymarin and pueraria isoflavone composite reagent. According to the prepared silymarin and pueraria isoflavone composite reagent, the silymarin dry powder and the pueraria isoflavone extract serve as core components, the silymarin dry powder can enhance liver detoxification and repair liver cells, the pueraria isoflavone extract can accelerate alcohol metabolism, and the silymarin dry powder and the pueraria isoflavone extract have a remarkable improvement effect on fatty liver, alcoholic liver and the like under the synergistic effect.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to the technical field of natural medicines, in particular to a preparation method of active ingredients in fructus psoraleae and application of the active ingredients in anti-inflammatory products, firstly, a fructus psoraleae reflux extract is properly enriched by using an extraction method, then selective separation is performed by using different separation modes of HSCCC, and the active ingredients in the fructus psoraleae are obtained. And 10 monomeric compounds are separated by combining a prepared liquid phase. Through cell experiments, four compounds of psoralen, neopsoralen isoflavone, psoralen flavanone and psoralen flavanone methyl ether, which can well inhibit NO release, are screened out; the four monomeric compounds can reduce secretion of proinflammatory factors by weakening mRNA transcriptional ability and weaken over-phosphorylation expression of pathway protein, so that an anti-inflammatory effect is achieved; through verification, the fructus psoraleae flavanone and the fructus psoraleae flavanone methyl ether can play an anti-inflammatory role through MAPK and NF-kappa B signal channels.

The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention discloses an application of polygonatum cyrtonema cyclase PcAS1 in promoting synthesis of hematoxylin A. The cyclase PcAS1 is derived from polygonatum cyrtonema, a high isoflavone compound hematoxylin A is synthesized in a plant body, and according to the application, a recombinant plant expression vector for expressing a PcAS1 gene is constructed and transformed into the plant to realize synthesis of the hematoxylin A. Based on multiple omics data such as whole genome, re-sequencing and transcriptome of polygonatum cyrtonema, key cyclase PcAS1 closely related to homoisoflavone biosynthesis is systematically mined. Through a tobacco transient expression system and a polygonatum cyrtonema hairy root transformation system, it is proved for the first time that cyclase PcAS1 can significantly promote synthesis of high isoflavone hematoxylon A, and biosynthesis of hematoxylon A is achieved in polygonatum cyrtonema for the first time.

Aspects of disclosure relate to a composition and methodology for treating menopause symptoms utilizing a cannabinoid-based nanoplatform composition. The composition includes phytocannabinoids like CBD, CBG, and CBN, alongside isoflavones and polyphenols, all integrated into nanoplatform designed for enhanced bioavailability and controlled release. The formulation is designed to alleviate key menopause-related issues, including vasomotor symptoms (VMS), genitourinary syndrome of menopause (GSM), bone loss, mood disturbances, and sleep disorders, while addressing cardiovascular disease (CVD) risks. The composition combines cannabinoids, such as CBD, with isoflavones and polyphenols, and is incorporated into nanoplatforms for enhanced delivery and efficacy. Additionally, the disclosure includes a kit comprising oral capsules, intravaginal ovules, and instructions for use. The capsules contain a blend of cannabinoids, flavonoids, and polyphenols, while the ovules are composed of CBD and cocoa butter. The kit provides a comprehensive solution for managing menopause symptoms and mitigating cardiovascular risks.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

This utility model relates to a germination device for enriching soybean isoflavones, comprising a box body, a perforated tray disposed within the box body, and an ultraviolet lamp assembly positioned above the perforated tray. The ultraviolet lamp assembly emits ultraviolet light of at least UV-B wavelength. A lifting assembly is provided between the ultraviolet lamp assembly and the box body, and / or between the perforated tray and the box body. A spray assembly and a circulating air duct assembly are provided within the box body. An air duct control assembly, connected to the circulating air duct assembly, is located outside the box body and is used to regulate the temperature and humidity within the box body. This utility model employs a closed or semi-closed design, with a built-in ultraviolet lamp assembly emitting UV-B wavelength ultraviolet light. The ultraviolet lamps can be used individually or in combination, facilitating the adjustment of UV-B irradiation intensity and its uniformity, meeting the ultraviolet irradiation intensity requirements of germinating soybeans at different growth stages, and increasing the isoflavone content in germinating soybeans.

The invention relates to the field of mineral flotation, in particular to a dihydroxythioisoflavone copper-arsenic separation inhibitor and a preparation method and application thereof. The preparation method of the copper-arsenic separation inhibitor comprises the following steps: adding an 8-beta-D-glucopyranosyl-4 ', 7-dihydroxyisoflavone organic compound into a solvent, then adding a Lawson reagent, raising the temperature, and reacting to obtain a reaction solution; and cooling, filtering, purifying and crystallizing the reaction liquid to obtain the dihydroxythioisoflavone copper-arsenic separation inhibitor. The dihydroxy thioisoflavone copper-arsenic separation inhibitor prepared by the invention can selectively carry out oxidation inhibition on arsenic-containing minerals such as arsenopyrite, arsenhedrite and akaidite, reduces the content of arsenic in copper concentrate, is simple to apply on site, does not need to change the process flow on site, only needs to adjust the dosage of the medicament and add the medicament according to the amount, is simple to operate, and is suitable for industrial production. The index is stable.

The application discloses a lactobacillus crispatus based on multiple targets to realize anti-aging effect and application of transformation of glycyrrhiza for efficiency enhancement, and belongs to the technical field of microorganisms and the technical field of medicine. The lactobacillus crispatus CCFM1362 provided in the application can ferment glycyrrhiza extract, can improve the content of glycyrrhizic acid, hemiglycyrrhizin isoflavone B, naringenin chalcone and other substances, can enhance the efficacy of glycyrrhiza extract, and can realize the effect of multiple targets for anti-aging, and the specific embodiments are as follows: (1) the content of beta-galactosidase in liver tissue is inhibited; (2) the level of aging-related secretory phenotype in liver tissue is reduced; (3) the Nrf2 related pathway is activated, and the level of oxidative stress is reduced; (4) the NF-kappa B related pathway is activated, and the level of chronic inflammation is reduced. Therefore, the lactobacillus crispatus CCFM1362 and the glycyrrhiza extract have great application prospect in the production of anti-aging products.

The present invention provides a protective film agent and a method for processing a workpiece, the protective film agent being capable of forming a protective film suitable for processing with a laser beam. The protective film agent is coated on a workpiece when the workpiece is processed, the protective film agent comprising: a water-soluble resin; a light absorber having a flavone structure, a flavonol structure, or an isoflavone structure; and a solvent that dissolves the resin and the light absorber.

This disclosure relates to a method for quantitatively detecting active ingredients in Ophiopogon japonicus and its application. The method includes: S1, placing the Ophiopogon japonicus to be tested in an extract, shaking, sonicating, centrifuging, and then mixing with an internal standard to obtain a test solution; S2, using liquid chromatography-mass spectrometry to detect the content of active ingredients in the test solution; the active ingredients include flavonoids and saponins. This method can simultaneously and rapidly quantitatively analyze multiple active substances in Ophiopogon japonicus, including steroidal saponins with different physicochemical properties, high levels of isoflavones, and ketones, which is of great significance for evaluating the quality level of Ophiopogon japonicus.

To improve the equol intake of an individual user.SOLUTION: A recommended amount output system includes one or more computers that perform the followings: calculating a user's equol deficiency amount using a predicted value of the user's equol production amount and a predetermined target amount; calculating a recommended amount of equol or isoflavone that the user should ingest based on the calculated equol deficiency amount and an index value representing the degree of the user's equol production ability; and outputting the calculated recommended amount.SELECTED DRAWING: Figure 3

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention provides an astragalus membranaceus calycosin-7-methyltransferase protein AmOMT1, a coding gene, a primer group and application of the astragalus membranaceus calycosin-7-methyltransferase protein AmOMT1, and belongs to the technical field of biology. The amino acid sequence of the astragalus membranaceus calycosin-7-methyltransferase protein AmOMT1 is as shown in SEQ ID No. 1 (sequence identifier number 1). According to the present invention, based on the radix astragali seu hedysari genome and the second-generation transcriptome data, the transmethylase AmOMT1 having 4 'and 7-site methylation functions on 3', 4 ', 7-trihydroxy isoflavone simultaneously is found and identified by using the reverse genetics method, and the experiment results show that the AmOMT1 can catalyze the methylation of the 3', 4 ', 7-trihydroxy isoflavone to generate calycosin and 3', 4 '-dihydroxy-7-isoflavone, and the transmethylase AmOMT1 can catalyze the methylation of the 3', 4 ', 7-trihydroxy isoflavone can catalyze the methylation of the 3', 4 ', 7-trihydroxy isoflavone to generate the 3', 4 '-dihydroxy-7-isoflavone.

The application relates to application of C-methylated high isoflavone compounds in preparation of antitumor drugs and belongs to the technical field of drug application. In order to solve the problem that the existing antitumor activity is not much reported, the application of C-methylated high isoflavone compounds in preparation of antitumor drugs is provided, the C-methylated high isoflavone compounds are selected from compounds shown in the following formula 1 or formula 2: the C-methylated high isoflavone compounds or pharmaceutically acceptable salts thereof are used as active ingredients for preparation of antitumor drugs for preventing and / or treating adrenal pheochromocytoma (PC-12) and / or human brain astrocytoma (U-87MG). The C-methylated high isoflavone compounds have the advantages of high activity and high inhibition capacity, can be extracted from polygonatum sibiricum and have good drug safety.

This invention discloses an isoflavone compound, its preparation method, and its applications. The compounds are derived by directional esterification modification of all phenolic hydroxyl groups with allyloxycarbonyl chloride, o-chlorobenzoyl chloride, or cinnamoyl chloride, using 4',7-dihydroxyisoflavone or 4',5,7-trihydroxyisoflavone as the parent nucleus. The invention also provides a mild and efficient preparation method for these compounds and confirms their significant anti-inflammatory pharmacological activity. Pharmacological studies show that compounds B and E, in particular, exhibit excellent in vitro and in vivo anti-inflammatory activity, with compound E showing superior activity compared to the positive control drug dexamethasone. These compounds can be used to prepare drugs for treating inflammatory diseases such as pneumonia and colitis.

The invention belongs to the field of functional food processing, and provides a preparation process of functional soybean milk powder with a sleep improving effect, which adopts a five-step linkage design of germinated soybean enzymolysis, lactobacillus plantarum fermentation, in-situ calcium nucleation, isoelectric point adsorption, exopolysaccharide stabilization and spray drying. Layered coating of the fermentation system autogenous exopolysaccharide and the fermentation modified soybean protein on the organic acid calcium microcrystal core is realized through pH gradient dynamic regulation and control, and a core-protein middle layer-polysaccharide outer layer ternary in-situ composite microcapsule structure is constructed. The multi-target synergy that the gamma-aminobutyric acid is enriched to 150-300 mg / 100 g, the isoflavone aglycone rate reaches 70-85% and the microcapsule D50 is controlled to be 5-80 microns is achieved, the three technical problems that high-solid-content low-viscosity spraying and strong interaction conflict exists, the dry powder flowability and core-shell layer stability conflict exists, and a narrow pH window and amplification tolerance are opposite are solved, and the method has wide functional food application value.

The application discloses a composition for treating lumbar intervertebral disc herniation and repairing annulus fibrosus, relates to the technical field of traditional Chinese medicine compositions, and comprises the following raw materials: radix rehmanniae preparata, plastrum testudinis, fructus psoraleae, eucommia ulmoides oliver, walnut kernel, bee pollen, ligustilide, saikosaponin A, salvianolic acid B, ferulic acid, calycosin, stibene glycoside, prunol, loganin, hydroxysafflor yellow A and ligustilide. Experiments show that the composition can strengthen the repair of annulus fibrosus, has anti-inflammatory and analgesic effects, protects collagen, inhibits degeneration, can effectively treat lumbar intervertebral disc herniation and realize the structural repair of damaged annulus fibrosus, and provides a new technical scheme for the clinical treatment of lumbar intervertebral disc herniation.

The invention discloses a glycosyl transferase combined mutant and application thereof, and aims to solve the problems that the existing wild PlUGT43 and F196Y single-point mutants thereof have narrow substrate spectrums and cannot efficiently catalyze a plurality of isoflavone derivatives with large structural differences to perform C-glycosylation reaction. The glycosyl transferase combination mutant provided by the invention is a protein which simultaneously contains five mutated sites, namely T81S, N186S, L187A, F196Y and S310P, on the basis of a wild PlUGT43 amino acid sequence as shown in SEQ ID NO.3. The glycosyl transferase combination mutant provided by the invention is a protein which is obtained by mutating five sites, namely T81S, N186S, L187A, F196Y and S310P. Compared with a wild type and an F196Y single-site mutant, the combined mutant has the advantages that the catalytic activity on classical substrates such as daidzein is improved, and efficient C-glycosylation on various substrates such as soybean dihydroaglycone, equol and resveratrol which are difficult to catalyze or extremely low in catalytic efficiency is also realized; the application potential of the glycosyl transferase in the aspect of synthesis of diversified natural products is improved.

To provide a technique for improving equol intake of individual users.SOLUTION: A recommended amount output system includes one or more computers that: acquire an index value representing a degree of equol production capacity of a target user and information representing a state of the target user; execute a determination process of determining a recommended amount of equol and / or isoflavone to be ingested by the target user on the basis of equol and / or isoflavone intake of other users whose information representing equol production capacity and state is similar to that of the target user, by using information of the other users stored in advance in a storage device, including an index value representing a degree of equol production capacity of the other users, information representing states of the other users, and information representing equol and / or isoflavone intake of the other users; and output the determined recommended amount.SELECTED DRAWING: Figure 6

A cosmetic lip care composition for improving the appearance of the lips, characterized in that it comprises at least one plant-based isoflavone component and a cosmetically compatible carrier base, wherein the composition is intended for topical application to the lips.

A small molecule composition, comprising licochalcone A, licochalcone C, licochalcone D, licochalcone E, echinatin, isoliquiritigenin, formononetin, liquiritigenin, licoflavone C, glabrol, licoisoflavone A and licoisoflavone B, and the use thereof in preparing anticancer or antitumor drugs, anti-ferroptosis drugs, or drugs for sensitization of anticancer or antitumor drugs. The small molecule composition can also be used in combination with other antitumor drugs.

The application discloses application of a Chinese medicine pair effective component composition of Astragalus membranaceus and Curcuma zedoary in preparation of anti-tumor metastasis drugs. The composition is composed of calycosin-7-glucoside and curcumin. The composition can effectively inhibit transendothelial invasion and vasculogenic mimicry generation of tumor cells at a non-toxic dose, and has the advantages of high efficiency and low toxicity in treating tumor metastasis. The important point of the application is that the preferred composition can significantly reduce the exosome LAP-TGF-beta1 protein loading level, thereby greatly inhibiting the tumor metastasis process caused by the exosome-mediated TGF-beta signal pathway. The finding provides a new perspective for the development of drugs for targeting exosome LAP-TGF-beta1 protein loading to resist tumor metastasis, and overcomes the defects of unclear effective components, uncontrollable proportioning and unclear treatment effect of traditional Chinese medicine compatibility. Therefore, the Chinese medicine effective component composition has application prospects in preparation of anti-tumor metastasis drugs.

The invention provides an anti-alcohol functional water based on liver enzyme activation, and relates to the technical field of functional water. The hangover alleviating functional water based on liver enzyme activation is prepared from radix puerariae isoflavone aglycone, globe artichoke water-soluble polysaccharide, emblic leafflower fruit procyanidine oligomer, an electrolyte combination, a pH buffer system, hovenia dulcis thunb, fructus gardeniae, beta-nicotinamide mononucleotide, hydrogen-rich water, vitamin C, vitamin E and curcumin, and the electrolyte combination is prepared from sodium chloride, potassium chloride and sodium bicarbonate. Alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver can be activated through the radix puerariae isoflavone aglycone, the globe artichoke water-soluble polysaccharide, the emblic leafflower fruit proanthocyanidin oligomer and the like, so that decomposition and metabolism of alcohol are accelerated, the retention time of the alcohol in the body can be remarkably shortened by activating the enzymes and dispelling drunkenness, and the symptoms of drunkenness and hangover are reduced.