64 results about "Dehydratase" patented technology

The invention discloses a production method of fucosylated oligosaccharide. The core of the method is as follows: the GDP-D-rhamnose is converted into the GDP-L-fucose by using the GDP-D-rhamnose-3, 5-epimerase; the GDP-D-rhamnose is obtained by converting the GDP-D-mannose-4, 6-dehydratase and the GDP-4-keto-6-deoxy D-mannose reductase by taking the GDP-D-mannose as a substrate, and the GDP-D-rhamnose is obtained by taking the GDP-D-mannose as a substrate. The invention relates to a method for producing a fucosylated oligosaccharide, which is characterized in that a GDP-D-mannose-4, 6-dehydratase, a GDP-4-keto-6-deoxyD-mannose reductase, a GDP-D-rhamnose-3, 5-epimerase and a fucosyltransferase are expressed in a genetically modified host cell, and the fucosylated oligosaccharide can be produced by the way mentioned above.

The invention provides a series of GDP-mannose dehydratase polypeptides which can be applied to synthesis of GDP-fucose, and belongs to the technical field of enzyme engineering. The GDP-mannose dehydratase polypeptide comprises a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to that of a polypeptide shown in SEQ ID NO: 1, or a polypeptide of which the amino acid sequence is at least 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to that of a polypeptide M1-13. The GDP-mannose dehydratase polypeptide disclosed by the invention has relatively high catalytic activity.

The invention discloses a recombinant bacterium capable of increasing the yield of alpha-bisabolol as well as a preparation method and application of the recombinant bacterium, and belongs to the technical field of microorganisms. The invention aims to improve the yield of alpha-bisabolol and enhance the tolerance of a host to an organic solvent. The invention provides a recombinant bacterium for improving the yield of alpha-bisabolol. Escherichia coli is used as a starting strain; the method comprises the following steps of: overexpressing an acetyl CoA acyltransferase / HMG-CoA reductase mvaE gene, an HMG-CoA synthetase mvaS gene, a 2-methyl citrate dehydratase prpD gene, a mevalonate kinase ERG12 gene, a mevalonate 5-phosphate kinase ERG8 gene, a mevalonate 5-diphosphate decarboxylase ERG19 gene and an isopentenyl diphosphate isomerase idi gene, so as to obtain a recombinant vector; the gene is obtained from an alpha-bisabolol synthase gene of artichoke, a farnesyl diphosphate synthase ispA gene and an alpha-bisabolol synthase CcBOS gene of artichoke. The industrial process of synthesizing alpha-bisabolol by a biological method is promoted.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

The invention discloses construction and application of genetically engineered bacteria for high-yield rose honey sweet essence. The genetically engineered bacterium is obtained by overexpressing tobacco pre-benzoic acid dehydratase NtPHA2 and an ARO80 mutant ARO80S706T / L877Q / E910D by virtue of saccharomyces cerevisiae. According to the invention, NtPHA2 and an ARO80 mutant ARO80S706T / L877Q / E910D are over-expressed in the saccharomyces cerevisiae, so that the biosynthesis yield of rose honey sweet aroma substances such as 2-phenethyl alcohol and the like is increased, and the rose honey sweet essence can be produced in batches by utilizing the engineering strain.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

This invention belongs to the fields of fermentation and genetic engineering technology, and provides a high-yield hexanoic acid-producing engineered *Escherichia coli* strain, its construction method, and its applications. This engineered strain is *Escherichia coli*... E. coli w3110 was the starting strain, overexpressing the acetyl-CoA transferase gene. act and β-ketothiolase gene bktB Overexpression of trans-enoyl-CoA reductase gene ter 3-Hydroxybutyryl-CoA dehydrogenase gene hbd, and 3-hydroxybutyryl coenzyme A dehydratase gene crt Or, simultaneously not expressing the pyruvate formate lyase gene. pflB or lactate dehydrogenase gene ldhA This engineered bacteria can convert glucose into hexanoic acid, a high-value product, with a yield of 4.968 g / L after 18 hours of fermentation. It adopts aerobic fermentation, resulting in rapid cell growth, a short fermentation cycle, and a high acid production rate. The fermentation process is simple, easy to control, and has low production costs, which is conducive to its promotion and application in industrial production.

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase / isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.

Disclosed in the present invention is the use of a threonine dehydratase in the regulation of the growth of Didymella segeticola and as a bactericide target. The threonine dehydratase gene Td of the present invention is derived from Didymella segeticola, and a knockout mutant is obtained by means of constructing a knockout gene fragment targeting the Td gene and then introducing same into a D. segeticola protoplast. Experiments find that the Td gene plays a role in the growth, the in-vivo pyruvic acid content and the in-vivo ATP content of D. segeticola, and the sensitivity thereof to wuyiencin. By means of molecular docking analysis, molecular dynamics simulation and microscale thermophoresis experiments, it is verified that threonine dehydratase can be used as a bactericide target, and the bactericide wuyiencin is obtained by means of screening. The threonine dehydratase of the present invention can be used as a bactericide target and a key protein in the pathogenic mechanism of a disease, and has broad application prospects in the control of plant pathogenic fungal diseases.

Provided are a genetically engineered bacterium for high-yield production of D-pantothenic acid (D-PA), and a preparation method therefor and a use thereof. The D-PA production yield of the engineered bacterium is significantly improved by means of the following actions: overexpressing acetolactate synthases IlvB and IlvN for pyruvate diversion while deleting a transcription attenuation region and mutating a valine feedback inhibition site; overexpressing ketol-acid reductoisomerase IlvC and dihydroxy acid dehydratase IlvD, and altering the coenzyme preference of IlvC from NADPH to NADH to unlock the upstream pathway for D-PA synthesis; overexpressing PanB and overexpressing a serine-glycine transport system to promote the synthesis of 5,10-methylenetetrahydrofolate; at the same time, enhancing the expression of ketopantoic acid reductase PanE; and finally overexpressing pantothenate synthetase PanC and increasing the content of cofactor ATP required by PanC. By means of the integrated enhancement of the above multiple modules, the provided engineered bacterium achieves improved cell growth due to balanced cofactors and increased D-PA.

The invention belongs to the technical field of gene engineering, and discloses a method for synthesizing L-cysteine through whole-cell catalysis, recombinant Escherichia coli and application, and the recombinant Escherichia coli is constructed through the following method: knocking out sdaA and tdcG genes for coding L-serine dehydratase; mutant beta subunit genes are obtained to construct recombinant plasmids, and the recombinant plasmids efficiently overexpress mutant genes and genes trpA in strains to obtain recombinant escherichia coli. The method is mild in reaction condition, low in energy consumption, environmentally friendly and high in substrate utilization rate through an enzyme method, and the overall cost is obviously reduced. The catalytic system can efficiently react at 25-35 DEG C from neutral to alkalescence, high temperature and high pressure or toxic chemical reagents are not needed, and energy consumption and environmental pressure are remarkably reduced. Meanwhile, the reaction system is high in substrate conversion rate and few in side reaction, the utilization rate of raw materials is greatly increased, and the method has obvious economic advantages.

This disclosure provides glucarate dehydratase enzymes useful for commercial scale production of 5-keto-4-deoxy glucaric acid (KDG) from glucaric acid. The produced KDG may be dehydrated to form furan dicarboxylic acid (FDCA). The disclosed enzymes ("dehydratase variants") are variants of Lactipantibacillus pentous dehydratase engineered to have improved thermal tolerance and resistance to inhibition by tartrate as compared with the parent enzyme.

The application discloses tobacco isopropyl malate dehydrase NtIPDS, a coding gene, an RNAi interference carrier and application. The application successfully clones a coding gene of isopropyl malate dehydrase in tobacco, and the nucleotide sequence is as follows: (1) the nucleotide sequence shown in SEQ ID NO. 1; (2) the nucleotide sequence shown in SEQ ID NO. 1 is substituted and / or deleted and / or added with one or more nucleotides and the nucleotide sequence of the same functional protein. Based on the existing tobacco genetic engineering progress and related plant phenotype observation and identification, the NtIPDS gene is deeply researched, and it is found that the NtIPDS gene is related to tobacco chloroplast development and acyl sugar metabolism. After the gene is silenced, the chlorosis plant growth is slow, the chloroplast development is found to be abnormal through transmission electron microscope observation, and meanwhile, it is found that the acyl sugar content of the silenced plant is significantly increased through metabolic detection analysis.

The invention provides a genetically engineered recombinant Klebsiella michiganensis having reduced or inactivated glucose-specific PTS transport system, glycerol kinase, glycerol dehydrogenase, alcohol dehydrogenase, acetolactate synthase, acetolactate decarboxylase, fumarate reductase and 3-phosphoglyceraldehyde dehydrogenase, having, preferably enhanced, NADPH-dependent alcohol dehydrogenase, diol dehydratase, 3-phosphoglycerate dehydrogenase and 3-phosphoglycerate phosphatase activities, methods of making said recombinant Klebsiella michiganensis, and uses and methods of producing 1,3-propanediol using said recombinant Klebsiella michiganensis.

The application relates to the technical field of microbial engineering, and particularly discloses a dihydroxy acid dehydratase mutant, a recombinant microorganism thereof and application. The dihydroxy acid dehydratase mutant takes the amino acid sequence of wild-type dihydroxy acid dehydratase as a reference sequence, and contains a mutation that the 237th alanine is substituted by lysine, arginine, histidine or proline. The fermentation bacteria with the mutant of the application have obviously improved L-valine production capacity, high yield and few by-products. The application provides a new high-efficiency production mode for fermentative production of L-valine.

A method for producing fucosylated oligosaccharides. The core of the method lies in converting GDP-D-rhamnose into GDP-L-fucose by using GDP-D-rhamnose-3,5-epimerase, wherein the GDP-D-rhamnose is obtained by means of converting GDP-D-mannose as a substrate via GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxy-D-mannose reductase. The expression of GDP-D-mannose-4,6-dehydratase, GDP-4-keto-6-deoxy-D-mannose reductase, GDP-D-rhamnose-3,5-epimerase, and fucosyltransferase in genetically modified host cells enables the production of fucosylated oligosaccharides via the described pathway.

The application discloses a method for fermenting 3-hydroxypropionic acid and acrylic acid. The application provides a recombinant Corynebacterium glutamicum, wherein the ald gene of the aldehyde dehydrogenase of the microorganism is up-regulated, the expression of the glycerolaldehyde-3-phosphate dehydrogenase gapA is down-regulated, the heterologous diol dehydratase gene pduCDEGH is expressed, the heterologous 3-phospho dehydrogenase gdp and glycerol 3-phosphatase gpp are expressed. The recombinant microorganism is fermented in a shake flask or a fermenter with glucose or other organic carbon sources as the substrate to obtain 3-hydroxypropionic acid. The 3-hydroxypropionic acid in the fermentation broth is acidified and heated to further obtain acrylic acid. The recombinant microorganism can efficiently produce 3-hydroxypropionic acid and acrylic acid by using cheap glucose, sucrose, molasses and the like as raw materials, the production process is green, safe and simple, and has a good market application prospect.

This invention discloses an engineered bacterial strain for producing xylitol using glucose as the sole carbon source, its construction method, and its applications, belonging to the field of genetic engineering. Using *Escherichia coli* as the starting strain, this invention optimizes the metabolic pathway by knocking out the 2-keto-3-deoxy-6-phosphate glucuronide gene (eda), knocking out the 6-phosphoglucuronide dehydratase gene (edd), and weakening the glucose phosphoisomerase gene (pgi), thus concentrating the carbon flow in the pentose phosphate pathway. Furthermore, it optimizes the metabolic pathway by knocking out or overexpressing endogenous genes such as the ribulokinase gene (araB). Subsequently, the synthesis of xylitol from glucose was experimentally tested by expressing a recombinant expression plasmid containing 2-arabinol dehydrogenase, 4-arabinol dehydrogenase, and xylitol dehydrogenase. This invention, through optimized metabolic pathways, efficiently converts glucose to xylitol without the need for glycerol addition, and significantly increases the yields of both the precursor arabinol and the product xylitol, demonstrating promising production prospects.

The present invention relates to the technical field of microbial metabolic engineering, and in particular to a Corynebacterium glutamicum strain capable of efficiently producing D-pantothenic acid, a construction method therefor, and the use thereof. The construction method specifically comprises the following steps: (1) using wild-type Corynebacterium glutamicum ATCC 13032 as a chassis, attenuating ilvA and performing mutation expression so as to construct DPA1; (2) knocking out pyruvate quinone oxidoreductase and inserting a panBCE gene expression cassette into same so as to construct DPA2; (3) introducing into the DPA2 an acetohydroxy acid synthase, an acetolactate isomeroreductase and a 2-hydroxyacid dehydratase which are overexpressed by using a pEC-xk99E overexpression plasmid and derived from Escherichia coli W3110 and Bacillus subtilis 168, and are endogenous and are obtained by screening; and (4) under the control of a strong promoter, subjecting the selected ilvBNCD gene to tandem overexpression by means of pEC-xk99E to construct DPA3, so that by enhancing the driving force in a principle synthesis pathway of pantothenic acid and upstream pathways, the Corynebacterium glutamicum strain capable of efficiently producing D-pantothenic acid is obtained. The strain provided by the present invention can use carbon sources to produce D-pantothenic acid with a high yield and high conversion rate.

This invention belongs to the field of genetic engineering technology and discloses a method for whole-cell catalytic synthesis of L-cysteine, a recombinant Escherichia coli, and its applications. The recombinant Escherichia coli is constructed by the following method: knocking out the enzyme encoding L-serine dehydratase. sdaA and tdcG Genes; obtaining mutant β subunit genes; constructing recombinant plasmids; efficient overexpression of mutant genes and genes in strains. wait Recombinant Escherichia coli was obtained. The method of this invention utilizes enzymatic reactions under mild conditions, low energy consumption, and is environmentally friendly, resulting in high substrate utilization and significantly reduced overall costs. The catalytic system can react efficiently under neutral to weakly alkaline conditions at 25-35°C, without requiring high temperatures, high pressures, or toxic chemical reagents, significantly reducing energy consumption and environmental impact. Simultaneously, the reaction system of this invention exhibits high substrate conversion and few side reactions, resulting in a substantial increase in raw material utilization and significant economic advantages.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

This invention discloses a method for preparing 1,2-pentanediol, comprising using 2-pentanone as a substrate, adding 2-keto reductase, linalool dehydratase, formate dehydrogenase, lysozyme, coenzyme NAD+, and water to react and obtain n-pentene. The obtained n-pentene is then thoroughly mixed with ethanol. This mixture is then fed into a reaction system containing olefin monooxygenase, epoxide hydrolase, formate dehydrogenase, lysozyme, coenzyme NAD+, MgSO4, and water using a fed-batch method. After the n-pentene and ethanol mixture has been completely added, the reaction continues for a period of time, followed by extraction to obtain 1,2-pentanediol. This preparation method uses 2-pentanone as a raw material, catalyzing the synthesis of 2-pentanol via 2-keto reductase. 2-pentanol is then catalyzed by a dehydrating enzyme to generate n-pentene, which is then catalyzed by an olefin monooxygenase to generate 1,2-epoxypentane. Finally, epoxide hydrolase ring-opens the 1,2-pentanediol. This process requires only four enzymatic catalytic reactions for efficient synthesis.

The invention discloses a genetically engineered bacterium for producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium, wild type C. glutamicum ATCC 13032 is taken as a chassis bacterium, an ilvE gene, an avtA gene, an ilvA gene and a pqo gene in an original strain genome are knocked out, ilvBNC from different sources and mutants thereof are screened, an optimized mutant ilvBNC-VWBGTG / ATG is determined, the gene cluster and promoters Ptuf and Pefu are connected and integrated to a strain DPAe-2, and the D-pantothenic acid is produced. An engineering strain DPAg-11 capable of efficiently accumulating KIV is obtained, then a promoter of an aceE gene is replaced by PdapA A16, KIV accumulated by the obtained engineering strain DPAg-15 reaches 3.99 g / L, panB, panC, panE or ilvC from different sources are screened, it is determined that BspanBC + EcpanE has the best effect, BspanBC + EcpanE is constructed on plasmids and then converted into various engineering strains, a promoter of an EcpanE gene is replaced by PdapB, and KIV accumulated by the engineering strain DPAg-11 and PdapA A16 reaches 3.99 g / L. The method has the advantages that the method is simple and easy to implement, a substrate channel can be built between dihydroxy acid dehydratase (DHAD) and KPR by polypeptide pair RIAD-RIDD, D-PA produced by the finally obtained strain reaches 3.21 g / L and is increased to 97.0 times that of DPA produced by a wild strain, and production of D-PA can be effectively promoted by the strategy of the built substrate channel.

The invention relates to a genetically engineered bacterium for synthesizing L-theanine by using D-xylose. The genetically engineered bacterium is a recombinant host bacterium containing a heterologous expression Weimberg metabolic pathway related gene, a glutamate dehydrogenase encoding gene gdhA and a gamma-glutamyl methylamine synthetase encoding gene GMAS. Wherein the Weimberg metabolic pathway related genes comprise a xylose dehydrogenase encoding gene xylB, a xylose lactonase encoding gene xylC, a 2-keto-3-xylan dehydratase encoding gene yjhG, a D-KDX dehydratase encoding gene xylX and a 2, 5-dioxovalerate dehydrogenase encoding gene xylA, and the host bacteria are escherichia coli. The strain realizes efficient biosynthesis of L-theanine from D-xylose through a non-phosphorylated xylose assimilation pathway, and the pathway only needs seven enzymatic steps to complete de novo synthesis of L-theanine, is the shortest synthesis pathway known at present, and has a wide resource utilization prospect.

The invention discloses an aldoxime dehydratase mutant and application of the aldoxime dehydratase mutant in catalytic synthesis of a dinitrile compound, which is characterized in that wild aldoxime dehydratase of SEQ ID NO: 2 is subjected to M29A and L318F double mutation and marked as TU-1. The mutant can efficiently catalyze oxidation of 5-hydroxymethylfurfural (HMF) to generate 2, 5-furandicarboxaldehyde (DFF), the DFF is further converted into 2, 5-furandicarboxaldehyde dioxime (DFFD) and a final target product 2, 5-dicyanofuran (DCF) under mild conditions, the reaction selectivity is high, and the yield is excellent. The invention provides a green and controllable technical scheme with remarkable industrial potential for preparing high value-added furan chemicals from renewable resources.

The invention provides recombinant escherichia coli for synthesizing vanillyl alcohol as well as a construction method and application of the recombinant escherichia coli. Wherein the recombinant escherichia coli comprises a host bacterium, and the host bacterium overexpresses a DAHP synthetase encoding gene AroGfbr and a 3-dehydroshikimic acid dehydratase encoding gene QuiC on an escherichia coli genome by using a pTrc promoter, so that sufficient supply of protocatechuic acid is realized; an alcohol dehydrogenase encoding gene ADH4 or ADH6 is introduced, so that the conversion speed of a toxic intermediate 3, 4-dihydroxybenzaldehyde is accelerated, and the fermentation density of the strain is improved; genes mtn and luxs of a methylation cofactor SAM regeneration pathway are over-expressed on a genome, so that the methylation efficiency is improved. Therefore, the recombinant Escherichia coli provided by the invention can efficiently synthesize vanillyl alcohol through the combination of the transformation strategies, and can efficiently accumulate 1.66 g / L vanillyl alcohol in a shake flask, and the yield of a 3L fermentation tank reaches 6.21 g / L. The method for producing vanillyl alcohol by using the recombinant strain provided by the invention has the advantages of low cost, controllable process, simplicity and convenience in separation and extraction, and facilitation of industrial production.

This invention discloses the application of threonine dehydratase in regulating the growth of Didymella segeticola and as a target for fungicides. The threonine dehydratase gene Td of this invention is derived from Didymella segeticola. A knockout gene fragment of the Td gene was constructed and introduced into *D. segeticola* protoplasts to obtain a knockout mutant. Experiments showed that the Td gene plays a role in the growth of *D. segeticola*, its pyruvate content, its ATP content, and its sensitivity to wuyimin. Molecular docking analysis, molecular dynamics simulation, and micro-thermophoresis experiments demonstrated that the threonine dehydratase can serve as a target for fungicides, and the fungicide wuyimin was screened and obtained. The threonine dehydratase of this invention can serve as a fungicide target and a key protein in the pathogenic mechanism of plant pathogenic fungi, showing broad application prospects in the control of plant pathogenic fungal diseases.

The application belongs to the technical field of biology, and provides a recombinant microorganism and application of the recombinant microorganism in preparation of 2'-fucosyllactose, wherein the recombinant microorganism carries nucleic acids encoding GDP-mannose dehydratase, GDP-L-fucose synthase and alpha-(1,2) fucosyltransferase. It is found that by making the microorganism express GDP-mannose dehydratase, GDP-L-fucose synthase and alpha-(1,2) fucosyltransferase and adjusting the copy number of the above genes, efficient production of 2'-fucosyllactose can be realized, and the production cost is low, the cycle is short, and the yield is high.

The invention relates to a genetically modified cell containing GDP-mannose dehydratase polypeptide, which can be applied to synthesis of human milk oligosaccharide HMOs, and belongs to the technical field of synthetic biology. The present invention provides a genetically modified cell comprising a recombinant nucleic acid sequence encoding a GDP-mannose dehydratase polypeptide (GDP-mannose dehydratase) having an amino acid sequence that is at least 70%, 75%, 80%, 85%, 90%, 95%, 99%, 100% identical to SEQ ID NO: 1 and / or polypeptides M1-M13, and a genetically modified cell having a fucosyllactose synthesis activity.

The invention discloses a recombinant yarrowia lipolytica strain Ylscu-03 for producing scutellarin as well as a construction method and application of the recombinant yarrowia lipolytica strain Ylscu-03, and belongs to the technical field of genetic engineering and metabolic engineering. The recombinant yarrowia lipolytica strain Ylscu-03 is constructed by the following steps: introducing SlA-Tsynth7, SUMO-FNS I-Tsynth27, VPRHX, Pleu-Pxo-Ptef, F7GAT-Tsynth2, UDPGDH-Tsynth8 and TAL-Tsynth7 into a recombinant yarrowia lipolytica engineering strain Ylscu2-00, replacing an original promoter of a yeast endogenous pre-benzoic acid dehydratase gene PHA2 with a core promoter pPHA2-50, and introducing BbXFPK-Tsynth22 and BsPTA-Tsynth7, so that the recombinant yarrowia lipolytica strain Ylscu-03 is obtained. And the yield of scutellarin produced by fermenting Ylscu-03 for 84 hours can reach 6407.63 mg / L.