10 results about "Xylonic acid" patented technology

The invention is suitable for the technical field of chemical synthesis, and provides a nitrogen modified metal composite material and a preparation method and application thereof. According to the method, primary biomass and derivatives thereof are carbonized in ethylene glycol containing a nitrogen precursor, meanwhile, bimetal is reduced and loaded on a nitrogen modified functional carbon carrier, so that an alloy catalytic site is formed, and the alloy catalytic site is beneficial to adsorption of monosaccharide, promotion of mass transfer of substances and promotion of monosaccharide oxidation into a final product saccharic acid; therefore, the monosaccharide oxidation reaction is efficiently carried out, and a theoretical basis is provided for directly synthesizing saccharic acid from agricultural wastes such as straw through chemical catalytic oxidation. The monosaccharide comprises xylose, glucose and the like; the product saccharic acid comprises xylonic acid, gluconic acid, glucaric acid and the like. Results show that the conversion rate of glucose is 100%, the yield of gluconic acid is 81.7%, and the yield of glucaric acid is 13.1%.

The application belongs to the field of natural medicines, and particularly relates to cyclic peptide natural products-xylonic acid peptides and the use thereof in the preparation of anti-parasitic drugs. Specifically, 12 cyclic peptide natural products containing xylonic acid structural units are disclosed, which exhibit different degrees of anti-parasitic activity in biological activity tests and have wide application value.

The application belongs to the technical field of medicine, and discloses application of uridine-5' monophosphate and a compound thereof in preparation of a medicine for enhancing the proliferation ability of male reproductive cells. It is found for the first time that the uridine-5' monophosphate has the proliferation ability on the male reproductive cells, and the proliferation effect is best when the concentration of the uridine-5' monophosphate is 10 μM, the proliferation effect on the mouse TM3 cells is increased by 52.87% compared with a control, and the proliferation effect on the mouse TM4 cells is increased by 53.57%. In addition, the uridine-5' monophosphate has a synergistic ability when being compounded with xylonic acid lactone, and further improves the proliferation effect on the TM3 cells, thereby laying a foundation for treatment of male reproductive system diseases in the future.

An efficient method for producing L-threonic acid from L-xylonic acid is disclosed.

This invention discloses a method for the biosynthesis of high-purity L-threonate magnesium, belonging to the field of food fortification agents and biochemical synthesis technology. The invention uses L-xylorate calcium solution as a substrate, and obtains a purified L-threose solution through oxidative cleavage. Then, a complex dehydrogenase system composed of arabinose dehydrogenase, xylose dehydrogenase, and aldose dehydrogenase synergistically catalyzes the oxidation of L-threose to L-threonate. Finally, L-threonate magnesium is prepared by salt formation with magnesium hydroxide, crystallization, and drying. The purity of the obtained product is greater than 99.0%, the oxalic acid residue is less than 0.01%, the process is safe and environmentally friendly, and suitable for industrial production.

Methods and engineered hosts are disclosed that convert a lignocellulosic xylose-containing biomass source into xylonic acid and / or xylonate, which can be further processed into other useful derivatives. In particular, an exemplary engineered Bacidiomycetes, e.g., R. toruloides, host produces / expresses one or more fungal enzymes that convert xylose into xylonic acid / xylonate. Methods of using such hosts to consume pretreated lignocellulosic biomass in combination with certain native promoters and heterologous genes are also described herein.

The invention discloses a method for improving acid production of cells through cooperation of diatom and gluconobacter oxydans, and belongs to the technical field of bioengineering. The diatom and the gluconobacter oxydans are simultaneously inoculated into a sugar-containing liquid culture medium and are aerated and stirred, and then fermentation is performed. According to the method, the gluconic acid or the xylonic acid is efficiently prepared through biotransformation of the diatom and the gluconobacter oxydans, the yield of the gluconic acid or the xylonic acid added with the diatom under the same condition is obviously higher than that of the gluconic acid or the xylonic acid not added with the diatom in each time period, efficient acid production of cells is achieved, and the method has good innovativeness and practical value.

The invention discloses a high-throughput screening method of a 2-keto-3-deoxy-D-xylonic acid dehydratase mutant library and an application of a mutant in biological synthesis of alpha-ketoglutaric acid. The directed evolution modification method comprises the following steps: carrying out fusion expression on a 2-ketone-3-deoxy-D-xylonic acid dehydratase coding gene (xylX), a Rex protein coding gene (rex) and an annularly arranged yellow fluorescent protein coding gene (cpYFP); the method comprises the following steps: firstly, carrying out primary screening on a 2-ketone-3-deoxy-D-xylonic acid dehydratase random mutant library by utilizing a flow cytometry technology, and screening according to a cell fluorescence value to obtain a mutant which possibly has relatively high catalytic activity; then, re-screening the mutants obtained by primary screening through a deep-well plate ultraviolet spectrum method, so as to obtain mutants with obviously improved catalytic activity; and finally, carrying out shake-flask culture on the mutant obtained by secondary screening, and determining the concentration of extracellular alpha-ketoglutaric acid by adopting a high performance liquid chromatography so as to determine the activity of the 2-keto-3-deoxy-D-xylonic acid dehydratase mutant. The high-throughput screening method disclosed by the invention is high in universality, high in sensitivity, simple, effective and high in throughput, and the screened 2-keto-3-deoxy-D-xylonic acid dehydratase mutant can be applied to biosynthesis of alpha-ketoglutaric acid.

The invention provides an ecologically fragile area vegetation intelligent restoration device and method.The method comprises the following steps that adhesive layer slurry, matrix layer slurry and seed layer slurry are sequentially sprayed and sown in an ecologically fragile area, the mass ratio of the adhesive layer slurry to the matrix layer slurry to the seed layer slurry is (3-5): (30-40): 10, and the adhesive layer slurry comprises a sodium silicate solution; the substrate layer slurry comprises water, soil powder, a bagasse fermentation product and a compound fertilizer, and the bagasse fermentation product is prepared by fermenting acetobacter xylinum; the seed layer slurry comprises seeds and the substrate layer slurry. According to the method, a sodium silicate solution is sprayed and sown on the ground of the ecologically fragile area, then matrix layer slurry is sprayed and sown to form a matrix layer, and acetic acid in the matrix layer slurry reacts with the sodium silicate solution to generate a sodium silicate gel bonding layer. The sodium silicate gel has strong viscosity and can fix the substrate layer on the ground surface.

A D-xylose dehydrogenase comprising an amino acid sequence that has at least 70% identity to the amino acid sequence according to SEQ ID NO. 2 or fragments thereof.