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Method for co-production of 1,3-propanediol and D-1,2,4-butanetriol

A technology of propylene glycol and Klebsiella, applied in botany equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of limiting xylose, high xylose price, and low conversion rate

Active Publication Date: 2020-08-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of xylose is high, and the conversion rate of xylose in the production process of 1,3-propanediol is low, which seriously limits the application of xylose in the production of 1,3-propanediol

Method used

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  • Method for co-production of 1,3-propanediol and D-1,2,4-butanetriol
  • Method for co-production of 1,3-propanediol and D-1,2,4-butanetriol
  • Method for co-production of 1,3-propanediol and D-1,2,4-butanetriol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Construction of recombinant Klebsiella pneumoniae

[0050] Specific steps are as follows:

[0051] Synthesize the gene yjhG (SEQID No.6) encoding xylose dehydratase derived from Escherichia coli (Escherichia coli); combine the synthesized gene kivD encoding 2-ketoisovalerate decarboxylase with the recombinant plasmid pEtac-kivD-tac -xdh (recorded in the literature "Jing P Y, Cao X, Lu X Y, etc. Modification of an engineered Escherichia coli by a combined strategy of deleting branch pathway, fine-tuning xylose isomerase expression, and substituting decarboxylase to improve1,2,4-butanetriol production[J].Journal of Bioscience and Bioengineering, 2018,126(5):547-552.", the nucleotide sequence of the tac promoter is shown in SEQ ID No.7) by restriction endonuclease Xba I After digestion with Nco I, ligation was performed to obtain the ligation product; the ligation product was transformed into Escherichia coli (Escherichiacoli) BL21 to obtain the transformati...

Embodiment 2

[0052] Example 2: Coproduction of 1,3-propanediol and D-1,2,4-butanetriol

[0053] Specific steps are as follows:

[0054] With Klebsiella pneumoniae (Klebsiella Pneumoniae) JI22914 as a control, the single colony of recombinant Klebsiella pneumoniae JI22914 / pEtac-yjhG-tac-kivD-tac-xdh obtained in Example 1 was streak-inoculated on LB solid medium cultured at 37°C for 12-14 hours to form a single colony; pick a single colony and inoculate it into LB liquid medium, cultivate it at 37°C and 100rpm for 12 hours to obtain a first-class seed liquid; inoculate according to 1% (v / v) Transfer the primary seed solution to the new LB liquid medium, cultivate it for 8h at 37°C and 100rpm to obtain the secondary seed solution; transfer the secondary seed solution to the In the fermentation medium, shake culture at 37° C. and 150 rpm for 48 hours to obtain a fermentation broth.

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Abstract

The invention discloses a method for co-production of 1,3-propanediol and D-1,2,4-butanetriol, belongs to the technical field of fermentation and the technical field of biology and provides a recombinant klebsiella capable of efficiently transforming glycerol and xylose to co-produce 1,3-propanediol and D-1,2,4-butanetriol. The recombinant klebsiella takes a klebsiella as a host and expresses a gene xdh of encoding xylose dehydrogenase, a gene kivD of encoding 2-ketoisovalerate decarboxylase and a gene yjhG of encoding xylonic acid dehydratase, and the recombinant klebsiella is inoculated intoa fermentation medium containing the glycerol and the xylose for fermentation for 48 h, so that the yield of the 1,3-propanediol in a fermentation liquid can reach 18.1 g / L, and the yield of the D-1,2,4-butanetriol can reach 3.3 g / L; and meanwhile, the transformation rate of the glycerol in the fermentation liquid can reach 45.3% and the transformation rate of the xylose can reach 12.3%.

Description

technical field [0001] The invention relates to a method for co-producing 1,3-propanediol and D-1,2,4-butanetriol, and belongs to the field of fermentation technology and the field of biotechnology. Background technique [0002] 1,3-propanediol (1,3-propanediol, 1,3-PDO) is a colorless, odorless, viscous liquid with a salty taste and hygroscopicity. In the polyurethane industry, it is often used as the raw material of polyester polyol, the initiator of polyether polyol and the chain extender of polyurethane, etc.; in the organic chemical industry, it is an important monomer and intermediate, the most important The purpose is to synthesize polytrimethylene terephthalate (PTT) as a polymer monomer. Therefore, the market capacity of 1,3-propanediol is huge. [0003] At present, 1,3-propanediol is often produced in industry by inoculating Klebsiella pneumoniae (Klebsiella Pneumoniae), which can produce 1,3-propanediol, into a fermentation medium containing glycerol for ferment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/74C12N15/53C12N15/60C12P7/18C12R1/22
CPCC12N9/0006C12N9/88C12N15/74C12P7/18C12Y101/01C12Y401/01071C12Y402/01082
Inventor 诸葛斌刘郁青李玉石陆信曜宗红
Owner JIANGNAN UNIV
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