The invention relates to the field of
fermentation processes, in particular to a method for preparing 1, 4-
butanediol through multi-strain synergistic
fermentation, which comprises the following steps: inoculating a first strain and a second strain into a culture medium containing glucose and / or
xylose for synergistic
fermentation, producing
ornithine by the first strain by taking glucose and / or
xylose as a substrate, and converting
ornithine into 1, 4-
butanediol by the second strain. The invention discloses 1, 4-
butanediol. Glucose is converted into
ornithine through the first strain, ornithine is converted into 1, 4-butanediol through the second strain, the first strain and the second strain are subjected to synergistic fermentation, preparation of 1, 4-butanediol through microbial synergistic fermentation is achieved, and higher metabolic capacity and higher production efficiency are achieved. The mixed
sugar of
xylose and glucose prepared from
biomass raw materials is used as a substrate for fermentation, so that the method has the advantages that the raw materials are easy to obtain, renewable and low in cost, and the production cost can be reduced.