195 results about "Pentose" patented technology

The invention relates to the technical field of food processing, in particular to oat fermented milk and a preparation method thereof, and the preparation method comprises the following steps: respectively preparing bacterial suspensions of TZ3-1-2, TG8-1-9 and QF-1-1; adding the TZ3-1-2 bacterial suspension and the TG8-1-9 bacterial suspension into the oat juice, and carrying out lactic acid bacteria fermentation to obtain a lactic acid bacteria fermentation product; adding the QF-1-1 bacterial suspension into the lactic acid bacteria fermentation product for yeast fermentation to obtain a yeast fermentation product; and carrying out after-ripening catalysis on the yeast fermentation product to obtain the oat fermented milk. The lactic acid bacteria TZ3-1-2 are pediococcus pentosaceus, and the preservation number of the lactic acid bacteria TZ3-1-2 is CGMCC (China General Microbiological Culture Collection Center) No.32259; the lactic acid bacteria TG8-1-9 are lactobacillus plantarum, and the preservation number of the lactic acid bacteria TG8-1-9 is CGMCC (China General Microbiological Culture Collection Center) No.32258. The lactic acid bacteria TG8-1-9 are lactobacillus plantarum. According to the method, mixed fermentation and two-stage sequential fermentation technologies are utilized, the fermentation time is shortened, meanwhile, the defects of single fermentation are effectively overcome, and finally the oat fermented milk which is excellent in taste and good in tissue state in all aspects is obtained.

The present disclosure provides a recombinant yeast host cell having a plurality of native and / or heterologous enzymes that function in an engineered metabolic pathway to convert fructose-6-phosphate to acetyl-coenzyme A. The recombinant yeast host cell can be used, for example, in a process for making acetyl-coenzyme A, acetone and / or isopropanol. The plurality of native and / or heterologous enzymes is activated, upregulated, or overexpressed. The plurality of native and / or heterologous enzymes comprises a phosphoketolase; and optionally an acetate kinase; and / or a phosphotransacetylase. The recombinant yeast host cell comprises at least one of: at least two copies of a heterologous nucleic acid molecule encoding the phosphoketolase; a native and / or heterologous enzyme that functions in an engineered non-oxidative pentose phosphate pathway, wherein the native and / or heterologous enzyme is activated, upregulated, or overexpressed; and / or a native and / or heterologous protein that functions in an engineered metabolic pathway to convert pantothenate into acetyl-coenzyme A, wherein the native and / or heterologous protein is activated, upregulated or overexpressed and comprises at least one of: FEN2 or CAB1.

The invention provides an NMN efficient synthesis strain and a construction method and production application thereof, and belongs to the technical field of engineering bacteria. According to the invention, firstly, an NMN synthesis route taking NAM as a substrate is constructed in escherichia coli, and specifically, NadV and PrsD128A are introduced into the escherichia coli, so that the NMN synthesis flux is improved; pnuC is introduced to promote the external arrangement of the NMN; the method comprises the following steps: knocking out PncC, NadR, UshA, NudC and MazG; pfkA and TalA are knocked out to improve the carbon flux of the pentose phosphate pathway. GDH1 and GDH2 are heterologously expressed in engineering bacteria to establish a reducing power balance system, the reducing power balance system is used for converting NADPH excessively accumulated in an NMN synthesis route into NADH, and meanwhile, an esaR-esaI group response element is introduced to dynamically regulate and control the system. The engineering strain constructed by the method can accumulate 3.0 g / L of NMN after 24-hour shake flask fermentation.

The invention provides a recombinant pichia kudriavzevii strain and application thereof, pichia kudriavzevii is taken as an original strain, and a reduced TCA pathway is introduced and comprises the following steps: overexpressing fumarate reductase FRD in the strain; weakening or inactivating an ethanol pathway, comprising: knocking out any one or a combination of at least two of encoding genes of ethanol dehydrogenase ADH1, ADH3 or ADH6; enhancing the PPP pathway in the pentose phosphate pathway of the strain, including: overexpressing glucose-6-phosphate dehydrogenase ZWF1 and / or 6-phosphogluconate dehydrogenase GND1; the method for reducing the activity of the malate dehydrogenase MDH2 in the TCA circulation pathway comprises the step of knocking out the coding gene of the malate dehydrogenase MDH2 in the TCA pathway. The pichia kudriavzevii is modified by using genetic engineering and metabolic engineering methods, so that the yield and the yield of succinic acid are increased, and the pichia kudriavzevii has important application prospects in industrial production.

The invention discloses a processing method capable of shortening the fermentation period and reducing the biogenic amine content for inoculated fermentation of smelly mandarin fish, and belongs to the field of food processing. The method comprises the following steps: (1) pretreating mandarin fish; (2) preparing fermentation liquor, wherein the fermentation liquor is prepared from NaCl, Chinese prickly ash, a leavening agent and water; the mass percentage concentration of each component is as follows: 0.1% of pepper, 4-8% of NaCl, 0.5-1.5% of a leavening agent and the balance of water; (3) fermentation process of smelly mandarin fish: completely immersing the fish body in the fermentation liquid, respectively fermenting for 6-12 days at the temperature of 5-20 DEG C, and collecting a smelly mandarin fish sample; and (4) performing vacuum packaging: performing vacuum packaging on the collected smelly mandarin fish samples, and performing cryopreservation at-18 DEG C. The leavening agents are lactobacillus sake, pediococcus pentosaceus, lactobacillus plantarum and mixed strains (lactobacillus sake, pediococcus pentosaceus and lactobacillus plantarum in a ratio of 1: 1: 1) respectively. The fermentation period of the siniperca chuatsi is shortened through inoculated fermentation, the content of biogenic amine is reduced, and the method has very high application and popularization value.

The invention discloses a method for promoting synthesis of microbial oil based on a light-operated gene and a photocatalyst, and belongs to the technical field of microbial oil. According to the invention, the oleaginous yeast Po1f-OptoZWF1 containing a light-operated gene expression system is constructed by adopting a molecular biological technology, and meanwhile, the oleaginous yeast is cultured by adopting a two-stage method (darkness and illumination). In the dark stage, sufficient nutrition is provided, and rapid proliferation of the oleaginous yeast is promoted; in the grease accumulation stage, illumination culture is converted, a photocatalyst is added, a photocatalyst-oleaginous yeast hybrid system and a light-operated gene expression system are constructed to inhibit the ZWF1 gene and a corresponding pentose phosphate pathway, and a photocatalytic material provides photo-induced electrons to promote regeneration of reducing power NADPH, so that grease synthesis is efficiently promoted. According to the method, through gene modification and a two-stage culture strategy, the yield of oil synthesis is remarkably increased, and the problems that existing oil-producing microorganisms are slow in proliferation and low in oil yield are solved.

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system, and a first separation system forming a pentose-rich stream and a pentose-lean stream. The pentose-lean stream may be introduced to a hydrolysis system forming a hydrolysate and the hydrolysate introduced to a second separation system forming a hexose-rich stream and a lignin stream. Additionally, at least one of the pentose-rich stream or the hexose-rich stream may be introduced to a bioreactor containing microorganisms configured to produce hydrocarbon fuels. Additionally, the present disclosure also relates to systems for the production of hydrocarbon fuels. A system may include a pretreatment system, a first separation system, a hydrolysis system, a second separation system, and one or more bioreactors. Alternatively a system may include a pretreatment system, a hydrolysis system, a sugar separation system, and one or more bioreactors.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The invention discloses pediococcus pentosaceus MPL5 and application thereof in promoting conversion of combined calcium in food into ionized calcium and improving calcium bioaccessibility, and belongs to the technical field of microorganisms. The pediococcus pentosaceus MPL5 provided by the invention can effectively promote combined calcium to be converted into ionized calcium (greater than 60%) in a culture solution or a composition fermentation solution, so that the bioavailability of calcium is improved; in addition, osteoporosis can be improved and conditioned, specifically, the bone mineral density, the number of bone trabecula, the connectivity of the bone trabecula and the connection density of the bone trabecula of an osteoporosis model mouse are remarkably improved, the separation degree of the bone trabecula and the index of a structural model are remarkably reduced, and therefore osteoporosis is reduced. The pediococcus pentosaceus MPL5 disclosed by the invention has a very wide application range in preparation of functional foods and health foods for promoting ionized calcium conversion and assisting in relieving osteoporosis, and is healthy and safe.

The invention belongs to the technical field of microbial fermentation, discloses a method for multi-bacterium synergistic fermentation of roxburgh rose juice, and particularly discloses a microbial agent which comprises rhizopus arrhizus CLF001 and at least one of pediococcus pentosaceus, lactobacillus plantarum, acetic acid bacteria, saccharomycetes and lactobacillus acidophilus. According to the microbial agent provided by the invention, the content of polysaccharide, polyphenol, brass and the like can be remarkably increased and the content of tannin in the roxburgh rose normal juice can be reduced by fermenting the roxburgh rose normal juice for 3 days by using the microbial agent, so that the fermented roxburgh rose normal juice does not feel obvious astringent taste, and the bitterness of the roxburgh rose normal juice is greatly improved; when the microbial agent is used for fermentation, polyphenol substances such as tannin with heavy astringent taste in the roxburgh rose normal juice are converted into non-astringent polyphenol substances, the taste of the normal juice is improved, the content of active ingredients is increased, for example, key ingredients (such as polyphenol, flavone and polysaccharide) capable of remarkably improving the antioxidant activity in the roxburgh rose normal juice can be used for improving the antioxidant performance of the roxburgh rose normal juice.

The invention discloses a method for efficiently synthesizing D-psicose from cane sugar by escherichia coli through double PTS transfer. E.coliJM109 (DE3) is used as a chassis host bacterium, and a D-psicose synthesis path based on a sucrose PTS transport system is established through co-expression of alsE, a6PP, scrA and scrB genes; by introducing the glpX [K29A] gene, a carbon source is driven to flow from a D-fructose PTS transport system to a D-psicose synthesis path; through knockout of pfkA and rpiA genes and inhibition of expression of fbaA genes, an endogenous glycolysis pathway and a phosphopentose pathway are adjusted, and the carbon flow entering tricarboxylic acid circulation is adjusted, and finally, the purpose that the D-psicose is efficiently synthesized from sucrose by escherichia coli through double PTS transport is finally achieved. Genetic engineering modification is carried out on wild escherichia coli, D-psicose is efficiently synthesized from sucrose by using a sucrose PTS and fructose PTS system, and a new scheme is provided for synthesis of D-psicose.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention discloses lactobacillus pentosus capable of converting various substances of dendrobium officinale to generate rich active small molecules and improving skin health, and belongs to the technical field of microorganisms. The lactobacillus pentosus (Lactobacillus pentosus) CCFM1482 obtained by screening can be used for producing various active substances by fermenting dendrobium officinale and regulating and controlling the synthesis of skin collagen factors, such as HSF cells (human skin fibroblasts), MDA (malondialdehyde), Nrf2 (nuclear factor E2 related factor 2) and HaCaT cells (human keratinocytes). Therefore, the lactobacillus pentosus CCFM1482 fermented dendrobium officinale kimura ex migo fermentation liquor has a huge application prospect in the preparation of products for preventing or treating the skin quality, and the dendrobium officinale kimura ex migo fermentation liquor can be used for preparing the products for preventing or treating the skin quality.

The invention belongs to the technical field of molecular biology and genetic engineering, and discloses a CRISPR / dCas9 vector for improving the yield of lactyl-N-tetrasaccharide of bacillus subtilis as well as a construction method and application of the CRISPR / dCas9 vector, and a CRISPR / dCas9 system is applied to production of lactyl-N-tetrasaccharide from bacillus subtilis 168 for the first time. According to the invention, pW-dcas9, pW-sgRNA and pW-sgRNA-zwf recombinant vectors are constructed, the transcriptional level of the zwf gene in a phosphopentose pathway is significantly reduced by 11.32 times by using a CRISPR / dCas9 system induced by xylose, and the metabolic flux of a competitive pathway is weakened. The yield of the lactyl-N-tetrasaccharide of the engineering strain obtained by the invention is greatly improved by 31% and reaches 7.25 g / l. Meanwhile, an effective method is provided for lowering metabolic flows of a cell wall competitive pathway and a glycolysis competitive pathway in a later period.

The invention relates to the field of food processing, and particularly discloses composite yeast for low-GI bread, a preparation method of the composite yeast and the low-GI bread. The low-GI bread composite yeast comprises the following raw materials in parts by weight: 4-5 parts of saccharomyces cerevisiae, 3-5 parts of lactobacillus plantarum, 1-2.5 parts of pediococcus pentosaceus, 100-150 parts of solid state fermentation pomace powder, 0.1-0.5 part of an enzyme inhibitor and 25-40 parts of millet resistant starch. The compound yeast is used for preparing the bread with the low GI value, and the prepared bread is low in glycemic index, good in expansibility, large in size, good in water binding capacity and not prone to hardening.

The invention relates to the technical field of synthetic biology, in particular to a squalene high-yield saccharomyces cerevisiae engineering bacterium and a construction method and application thereof.Saccharomyces cerevisiae CEN.PK.2-1D is used as an original strain, pentose phosphate pathway genes ZWF1, GND1, TAL1 and TKL1 and IDP1 genes of TCA circulation are integrated in a genome of the original strain through homologous recombination, and the squalene high-yield saccharomyces cerevisiae engineering bacterium is obtained. Constructing a dual-channel cofactor supply system to improve the intracellular NADPH level and the NADPH / NADP + ratio; according to the saccharomyces cerevisiae genetic engineering strain Sc-Sq04 constructed by the method disclosed by the invention, the yield of squalene obtained by shake flask fermentation reaches 741.08 + / -28.9 mg / L by further integrating all genes of an MVA way, ERG20 and ERG9 genes, and a new thought is provided for transforming saccharomyces cerevisiae to synthesize terpene compounds and promoting industrial application.

The invention provides application of aspergillus aculeatus in fermentation processing of traditional Chinese medicine indigo naturalis. The invention further provides application of the combined use of the pediococcus pentosaceus and the aspergillus aculeatus in fermentation processing of the traditional Chinese medicine indigo naturalis. The combination of the aspergillus aculeatus and the pediococcus pentosaceus has obvious advantages in the fermentation of the indigo naturalis. The invention also provides a preparation method of indigo naturalis. In the aspect of an enzyme system, beta-glucosidase generated by the aspergillus aculeatus and the pediococcus pentosaceus can promote dissolution of an indigo precursor substance indole glycoside. In addition, other specific enzymes, such as glucoside hydrolase or oxidoreductase and the like, can also be generated, complex components in indigo naturalis are further decomposed through mutual cooperation of the two enzymes, more precursor substances are released, generation and accumulation of indigo blue and indirubin are jointly promoted, and the content of indigo blue and indirubin is increased. In addition, growth and metabolism of harmful microorganisms can be inhibited, harmful substances possibly generated in the indigo naturalis fermentation process are reduced, and the quality and safety of indigo naturalis products are improved.

The invention provides pediococcus pentosaceus King33 for relieving excrement odor of pet dogs and cats as well as a microbial agent and application of the pediococcus pentosaceus King33, and belongs to the technical field of microorganisms. The preservation number of the pediococcus pentosaceus King33 is CGMCC (China General Microbiological Culture Collection Center) No.31050, the pediococcus pentosaceus King33 is classified and named as pediococcus pentosaceus, and the preservation date is June 24, 2024. According to the pediococcus pentosaceus King33 disclosed by the invention, the odor in cat excrement can be reduced, and the excrement quality is improved; according to the pediococcus pentosaceus King33 disclosed by the invention, the content of volatile organic compounds and biogenic amines in cat feces can be reduced; the pediococcus pentosaceus King33 disclosed by the invention can be used for relieving body odor, halitosis and urine odor of dogs.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

The invention belongs to the field of microorganisms, and particularly relates to pediococcus pentosaceus capable of producing various pyrazines and application of the pediococcus pentosaceus. The strain is separated from distiller's yeast of Baijiu, and the preservation number of the strain is GDMCC NO: 65815. Tests prove that the strain can generate special pyrazine compounds, such as 2-methylpyrazine, other strains do not generate the special pyrazine compounds, and the 2-methylpyrazine has typical nut and baked food fragrance and can be used in the field of food processing of wine brewing, spices and the like.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

The invention discloses a method for distinguishing a vibrio alginolyticus wild type virulent strain and a cya gene deletion type low virulent strain based on proteomics. According to the method, a peak area ratio of ATP synthase subunit alpha (atpA) to phosphopentose mutase (deoB) in vibrio alginolyticus, which is measured by proteomics, is utilized to judge an adenylate cyclase coding gene cya-deleted attenuated strain and a wild virulent strain, the vibrio alginolyticus cya gene deleted attenuated strain with a result of atpA / deoB in a sample to be detected being less than 0.2, and the vibrio alginolyticus cya gene deleted attenuated strain with a result of atpA / deoB in the sample to be detected being less than 0.2 is a wild virulent strain with a result of atpA / deoB in the sample to be detected being less than 0.2. The vibrio alginolyticus strain with the result of atpA / deoB being 1.2 or above is a vibrio alginolyticus wild type virulent strain. The distinguishing method is easy to operate, high in detection speed and reliable in result.

The invention belongs to the technical field of food fermentation, and provides a naan air-holding aroma-enhancing rapid fermentation composite leavening agent and a preparation method, the composite leavening agent comprises freeze-drying type lactobacillus plantarum, freeze-drying type saccharomyces cerevisiae, freeze-drying type pediococcus pentosaceus, lactobacillus rhamnosus, lactobacillus casei and Xiangba powder, and the optimal mass ratio of the freeze-drying type lactobacillus plantarum, the freeze-drying type saccharomyces cerevisiae, the freeze-drying type pediococcus pentosaceus, the lactobacillus rhamnosus, the lactobacillus casei and Xiangba powder is 2: 1: 3: 2: 3: 1 or 3: 1: 3: 1: 2: 2. The crusty pancake prepared according to the proportion has better sensory characteristics, specifically, the crusty pancake tastes softer and more elastic, the surface glossiness is improved, and the color is more uniform and natural. In addition, the method can also shorten the fermentation time, and endows the product with unique aroma of the sour dough fermented crusty pancake, thereby enhancing the flavor of the final product. The problems that the taste and the flavor are single when commercial yeast is independently used for making the fermented crusty pancake are solved, and the problems that when traditional sour dough is used for making fermented flour products, the fermentation time is long, the stability is poor, the quality is difficult to control, harmful microorganisms are prone to breeding and the like are solved.

The invention relates to the technical field of raw materials of skin care products, and particularly discloses a soothing and moisturizing compound as well as a preparation method and application thereof. The preparation method of the soothing and skin-moistening compound comprises the following steps: 1) mixing rice, cornflower, quisqualis indica, cashew nut and water, heating and extracting to obtain a pre-extract; (2) adding compound bacteria into the pre-extract, and fermenting to obtain a fermented product; the invention discloses a soothing and skin-moistening compound and a preparation method thereof.The soothing and skin-moistening compound has the advantages that the soothing and skin-moistening compound is prepared from compound bacteria of streptomyces cinereorubens fructose fermentation subspecies, fermented lactobacillus mucus and pediococcus pentosaceus, and the soothing and skin-moistening compound is more remarkable in soothing effect.

This invention relates to the field of pancreatic extract technology, and discloses a pancreatic enzymatic hydrolysate, its preparation method, and its application, comprising the following steps: using porcine pancreas as raw material, 10% of the total mass of porcine pancreas is used to prepare a fermentation substrate; the fermentation substrate and the remaining porcine pancreas are enzymatically hydrolyzed, followed by solid-liquid separation and drying to obtain the pancreatic enzymatic hydrolysate; a fermenting agent is added during the preparation of the fermentation substrate, wherein the fermenting agent includes Pediococcus pentosaceus and Lactobacillus sakei. The pancreatic extract of this invention can alleviate inflammation.

This invention provides a method for the co-production of organic acids and lignin from lignocellulose, comprising the following steps: Step 1, pretreating the lignocellulose raw material to obtain a pretreated material; Step 2, enzymatically hydrolyzing the pretreated material to obtain an enzymatic hydrolysate containing pentoses, hexoses, and lignin; Step 3, performing solid-liquid separation on the enzymatic hydrolysate to obtain an enzymatic hydrolysate containing pentoses and hexoses and a solid phase containing lignin; Step 4, subjecting the enzymatic hydrolysate containing pentoses and hexoses to acid-producing fermentation to obtain a fermentation broth containing a mixture of organic acids; Step 5, extracting lignin from the lignin-containing solid phase to obtain lignin. The method provided by this invention can efficiently separate lignin and obtain various organic acids through a simple process.

Cosmetic products containing specific amounts of adenosine and at least one pentose in a cosmetic carrier are suitable for the treatment of hair and / or scalp and lead to - Revitalization of the hair and / or - Stimulation / activation of energy metabolism in hair follicles / hair roots and / or - Stimulation / enhancement of hair growth and / or - Prevention / reduction of premature hair loss and / or - Delaying scalp aging and / or - Increased keratin synthesis and / or - Improvement of the tensile strength of keratin fibers.

The application discloses a preservative for reducing the content of polycyclic aromatic hydrocarbons in meat products and application thereof. The preservative comprises Lactobacillus pentosus R3, the Lactobacillus pentosus R3 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No. 26346, and the preservation date is December 27, 2022. The preservative is inoculated on the surface of the meat product, the growth of spoilage microorganisms can be inhibited, fat oxidation of the meat product is delayed, the shelf life of the smoked meat product is prolonged, the content of polycyclic aromatic hydrocarbons in the smoked meat product can be significantly reduced, and the food safety is improved.

The present disclosure provides genetically engineered microorganisms for the simultaneous fermentation of pentose and hexose sugars, for example, glucose and xylose. The microorganisms can be modified to express AtSWEET polypeptides, LST1 polypeptides, mutants thereof, homologs thereof or combinations thereof. Also provided are methods of co-fermenting hexose and pentose sugars, methods of increasing the conversion of lignocellulosic biomass via microbial fermentation, and methods of generating biofuel.

The present invention belongs to the field of biotechnology, and particularly relates to a bionic synthesis engineering bacterium of compound Danshen and its application. The engineering bacterium is obtained by introducing cytochrome P450 reductase, tyrosine hydroxylase and D-lactate dehydrogenase of Lactobacillus pentosus on the basis of a Saccharomyces cerevisiae starting bacterium that produces protopanaxadiol, cryptotanshinone diene and borneol. The obtained Saccharomyces cerevisiae genetic engineering bacterium can simultaneously synthesize the active ingredients of compound Danshen preparations, namely protopanaxadiol, cryptotanshinone diene, danshensu and borneol, and has application value.