144 results about "Pediococcus" patented technology

The invention discloses a lactic acid bacteria semi-solid fermentation product. The product is prepared by the following processes: inoculating lactic acid bacteria in raw materials and fermenting at a temperature of 24-44 DEG C for 24-40h to obtain the product. The raw materials comprise 4.0-5.0g of wheat bran, 1.2-1.8g of soybean meal, 0.8-1.2g of glucose or brown sugar, and water, wherein water is added until the total amount of the raw materials is up to 100mL. The inoculation amount of the lactic acid bacteria is 1.0-2.0*10<7>cfu/100mL, wherein the lactic acid bacteria is composed of Lactobacillus plantarum and Pediococcus pentosaceus with a strain viable count ratio of (3:10)-(10:3). The lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals provided by the invention is the first fluid fermentation feed independently developed in China according to the national conditions for culture, inherits the advantages of the traditional solid fermentation feeds, and at the same time has the advantages of being more convenient, fresher, more significant in feeding effects, lower in cost and the like.

A composite preparation for reducing a diarrhea rate of weaned piglets is disclosed. The composite preparation is prepared by mixing an oligosaccharide-acidifier solution and a probiotic fermentation liquor according to a volume ratio of 1:(3-5). Each mL of the oligosaccharide-acidifier solution contains 2500-3500 mug of oxalic acid, 30-60 mug of tartaric acid, 50-100 mug of malic acid, 4000-5500 mug of lactic acid, 2500-4000 mug of citric acid, 0.05-0.2 g of glucose, 0.05-0.1 g of isomaltooligosaccharide, 0.02-0.06 g of fructooligosaccharide and 0.08-0.15 g of chitosan oligosaccharide. The probiotic fermentation liquor is prepared by mixing a mixed fermentation liquor of lactobacillus plantarum and pediococcus pentosaceus and a clostridium butyricum fermentation liquor according to a volume ratio of (2-4):1. A preparing method of the composite preparation is also disclosed. The composite preparation reduces the diarrhea rate of weaned piglets, reduces the death rate of piglets, and boosts productive performance and immunity of piglets.

The invention relates to a compound biological deodorizer as well as a preparation method and application thereof. The compound biological deodorizer comprises a plant extract, pichia pastoris, pediococcus pentosaceus, pediococcus acidilactici, candida mycoderma and bacillus megatherium. Through the utilization of compound biological deodorizer to treat a waste transfer station, a livestock and poultry farm and a composting site, the NH3 removal rate can respectively reach 65.2-71.6%, 63.4-73.7% and 53.42-75.77%, and the H2S removal rate can reach 53.5-60.1%, 35.5-45.9% and 47.2-53.8%, so that the odor concentration is obviously reduced, the environment of the periphery of the waste transfer station, the livestock and poultry farm and the composting site is effectively improved, and the improvement of both economic benefits and environment-friendly benefits is facilitated.

The invention provides a method for processing fermented fish. The method comprises the following steps: (1) removing the heads, tails and viscera of fresh fish, and dicing; (2) pickling the fish dices with a saline solution; (3) dewatering the pickled fish dices; (4) adding ginger power, paprika, red kojic rice, purple perilla powder, cane sugar, dextrose and white spirit to the dewatered fish dices, and fully stirring; (5) activating Lactobacillus plantarum, Pediococcus pentosaceus and micrococcus so as to be used as leavens in the processing; (6) mixing the Lactobacillus plantarum, the Pediococcus pentosaceus and the micrococcus, adding the mixture to the fish dices subject to dosing, and fermenting; and (7) finishing the fermentation so as to inactivate the leavens, and then stopping the fermentation. The method for processing the fermented fish has the advantages of controllable fermentation conditions, high and stable product quality, high product safety, and good organoleptic quality.

The invention discloses a protein feed raw material prepared from Asian carps and is used for animal breeding and a preparation method for the protein feed raw material. The preparation method comprises the steps of performing smashing pretreatment on the Asian carps, mixing the smashed Asian carps with a microorganism preparation, and then performing fermentation so as to obtain the high-quality protein feed raw material with balanced protein and amino acid. The protein feed raw material can be widely applied to livestock breeding and animal aquaculture. Strains of a microorganism leavening agent comprise at least three of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, lactobacillus acidophilus and pediococcus pentosaceus. A method which is low in energy consumption and low in cost and does not have any waste emission is supplied to deep processing of the Asian carps, so that the ecological problem caused by the Asian carps in America can be effectively controlled, and protein resources of the Asian carps are fully used; the prepared protein feed raw material can guarantee no loss of nutrition of the raw material, and polypeptides, cellulose, oligopeptide, oligopeptide mixtures, trace elements and the like can be generated; the optimal nutrition is supplied to animal breeding.

The invention discloses a fermentation method of chrysanthemum broad bean sauce and belongs to the field of bioengineering. The fermentation method of the chrysanthemum broad bean sauce comprises thefollowing steps: (1) performing pretreatment on broad beans and performing pretreatment on chrysanthemum flowers; (2) making starters; (3) adding all the broad bean starters into a fermentation tank,adding saline water and chrysanthemum flower pulp, stirring uniformly and performing fermentation for 35 to 40 DEG C, wherein weissella confuse, saccharomyces rouxii, pediococcus pentosaceus LBST-L2 and bacillus tequilensis are added in the fermentation process and fermentation is conducted for 30 to 40 days. According to the fermentation method of the chrysanthemum broad bean sauce, the fermentation state of the broad bean sauce under the natural environment is simulated, the obtained broad bean sauce has strong flavor, different cultures are added at different stages, the maturation processof the broad bean sauce is accelerated, the fermentation cycle is shortened to 30 to 40 days, the beneficial active components are increased and the nutritional requirements of modern people are met.

The invention discloses preparing and application of a probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in a chicken. Traditional Chinese medicines comprise artemisia apiacea, antifebrile dichroa, herba verbenae, phellodendron, radix sophorae flavescentis, radix curcumae, cape jasmine, angelica sinensis, pericarpium citri reticulatae, honeysuckle and agrimonia pilosa. Effective traditional Chinese medicinal ingredients are extracted by three times of decoction; afterwards, a traditional Chinese medicinal culture medium substrate is prepared from the effective traditional Chinese medicinal ingredients; lactic acid bacteria PP (pediococcus pentosaceus) are inoculated in the traditional Chinese medicinal culture medium substrate; the inoculated traditional Chinese medicinal culture medium substrate is cultured to prepare the probiotics-fermented traditional Chinese medicinal preparation for preventing and treating the coccidiosis in the chicken. In comparison with the prior art, according to the preparing and the application of the probiotics-fermented traditional Chinese medicinal preparation for preventing and treating the coccidiosis in the chicken, a probiotics-fermented traditional Chinese medicinal aqueous extract is used as a target composition; not only are the further conversion and decomposition of a polysaccharide, starch, cellulose and pharmaceutical active ingredients in the traditional Chinese medicines promoted and are more effective active ingredients are generated, but also the activity of probiotics is improved; the growth and the release of the probiotics and metabolites thereof are facilitated; further, an anticoccidial treatment effect is obviously higher than that of a pure traditional Chinese medicinal preparation.

The invention relates to a method for enhancing the flavor of Cantonese style sausage through using a biological flavoring process. Gig hindquarter meat with a fat to lean ratio at (2:8)-(3:7) is taken as a raw material, and accessories consist of common salt, sugar, ascorbic acid, light soy sauce, taste essence, sodium nitrite, a compound enzyme and pediococcus pentosaceus. The preparation method comprises the following steps of: separating fat and lean, and washing and cleaning; adding a curing agent into minced raw material meat, uniformly mixing, and curing for 1-4hours at 0-4 DEG C; inoculating the pediococcus pentosaceus with the strain concentration being 104-105cfu/ml; filling and fermenting for 2-10h at 30-50 DEG C; and drying until the water content of the sausage is lower than 20% to obtain the sausage. In the method, the compound enzyme is applied to the sausage, so that the sausage is properly oxidized to form an aromatic component; and the pediococcus pentosaceus participates in fermentation can be used for enhancing the aroma, and the over oxidation of lipid is inhibited. The Cantonese style sausage prepared by using the method is intense in flavor, pure in aroma and stable in quality.

Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

The invention provides a processing method of ramie and pennisetum purpureum mixed silage, and belongs to the field of feed additives. The processing method comprises the following specific steps: mixing 60%-80% of pennisetum purpureum and 20%-40% of ramie to obtain silage; then spraying fermentation liquor to the silage, spraying an additive at the same time, and then performing fermentation. Thefermentation liquor comprises compound bacteria, compound enzyme and water, and the compound bacteria comprise lactobacillus plantarum, lactobacillus buchneri, pediococcus pentosaceus and bacillus subtilis; the compound enzyme comprises cellulase, xylanase and laccase; and the additive comprises calcium propionate, molasses, urea, ethanol and water. According to the processing method of the silage, ramie and pennisetum purpureum mixed silage is adopted, nutrients are complemented, the fermentation liquid and the additive are combined for fermentation, the defect that ramie and pennisetum purpureum silage is difficult to silage is overcome, the dry matter and crude protein content and lactic acid bacteria of the silage are increased, and the crude ash content, the acid washing fiber content, the lignin content and the pH value are reduced.

The invention discloses a preparation method and application of a fermented Chinese herb biological fodder for ruminant animals. The Chinese herb biological fodder is prepared from the following raw materials of 21 to 29 parts of radix astragali seu hedysari, 18 to 32 parts of poria cocos, 22 to 28 parts of radix codonopsis, 9 to 18 parts of wormwood and 10 to 20 parts of liquorice root; aspergillus oryzae, bacillus natto and phaffia rhodozyma are inoculated for aerobic fermentation, and then enterococcus faecium and pediococcus pentosaceus are inoculated for anaerobic fermentation to obtain the fermented Chinese herb biological fodder. According to the fermented Chinese herb biological fodder disclosed by the invention, Chinese herb raw material compatibility is reasonable; by means of step-by-step fermentation of beneficial microorganisms, pharmaceutical functions of the Chinese herbs are obviously improved; meanwhile, a lot of metabolite is generated, the rumen environment of the ruminant animals is improved, the fodder utilization rate is improved, diseases are reduced, the fodder cost is reduced, and the economical benefits are improved.

The invention belongs to the technical field of animal feeds and bioengineering, specifically relates to a method for producing probiotic peptides by using the two-step solid-state fermentation of peanut meal. The method comprises the following steps: using the peanut meal as a raw material, killing bacteria after uniformly mixing and churning the peanut meal and water, adding 2%(v/w) aspergillus niger(CGMCC9183) spore liquid to the water solution of the peanut meal and the water according to the weight of the peanut meal after killing the bacteria, culturing the mixture of the spore liquid and the water solution for 24-28 hours at the temperature of 28-32 DEG C, and turning over and mixing the materials once every four hours; and adding 10%(v/w) pediococcus pentosaceus(CGMCC9182) to the mixture of the spore liquid and the water solution according to the weight of the peanut meal after culturing the mixture of the spores liquid and the water solution, uniformly mixing the mixture of the peanut meal, the water, the spore liquid and the pediococcus pentosaceus, anaerobically fermenting the mixture of the peanut meal, the water, the spore liquid and the pediococcus pentosaceus for 36-42 hours at the temperature of 37-46 DEG C, drying and crushing the mixture of the peanut meal, the water, the spore liquid and the pediococcus pentosaceusat the temperature of 60 DEG C after fermenting, and obtaining the probiotic peptides. The polypeptides content of the probiotic peptides achieves more than 25%, the lactic acid content achieves more than 8.0%, the viable bacteria count of lactobacilli is more than 109CFU/g, and acidic proteinases achieve more than 200U/g. The method disclosed by the invention has the advantages that the cost of production is low and the fermenting process is easy to control, so that the quality of products is stable.

The invention belongs to the technical field of microorganism, and in particular relates to high cohesion pediococcus pentosaceus screened from fermented bean products and use thereof in purifying of vibrio parahaemolyticus in water body. The pediococcus pentosaceus F28-8 is preserved in China General Microbiological Culture Collection Center in 2014 November 13, and the preservation number is CGMCC No.9956. The pediococcus pentosaceus has high cohesion activity, can be used for bacteria-reducing and purifying treatment of vibrio parahaemolyticus in water body to avoid use of a chemical antibacterial agent and antibiotics for water bacteria-reducing and purifying treatment, provides new means for breeding and biological control of vibrio parahaemolyticus in temporary rearing water, and is conducive to the prevention of the spread of aquatic animal disease and food borne diseases caused by the vibrio parahaemolyticus.

The invention relates to the field of microorganisms, particularly an active microorganism combination biological agent and a preparation method thereof. The active microorganism combination biological agent is prepared by mixing Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, Bacillus amyloliquefaciens, Bacillus subtilis, Lactobacillus acidophilus, Pichia farinosa, Pichia membranifaciens, Dekkera anomala and Dekkera bruxellensis. The biological agent has the advantages of high treatment efficiency for pollutants, favorable treatment effect, low treatment cost and no secondary pollution.

It is to provide a composition derived from lactic acid bacteria having an anti-allergic effect (an effect of suppressing allergic symptoms such as hay fever) synergistically, and a food product, pet food, etc. containing the composition. It is to prepare a composition having an anti-allergic action, containing Lactobacillus crispatus KT-11 strain, KT-23 strain, or KT-25 strain bacterial cells and viable bacteria, dead bacteria, or a processed product of bacterial cells of other lactic acid bacteria belonging to the genus Lactobacillus, genus Bifidobacterium, genus Leuconostoc, genus Enterococcus, genus Pediococcus, etc. at a biomass-converted ratio of 99:1 to 50:50, preferably 99:1 to 75:25.

Compositions and methods are provided for novel guide RNA/Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.

The invention belongs to the technical field of flower cultivation and in particular relates to a preparation method of a biogas slurry nutrient capable of improving bioactive components of rose flowers. The preparation method specifically comprises the following steps: (1) weighing raw materials including biogas slurry, grape peel residues, fresh sweet potato leaves, fresh mulberry leaves, garlicstraw, radix morindae officinalis, radix rehmanniae, pediococcus pentosaceus and water in parts by weight; (2) obtaining fermented pulp; (3) obtaining a fermented solution; (4) carrying out magnetization treatment on the fermented solution to obtain the biogas slurry nutrient, wherein the biogas slurry nutrient contains abundant nutrient substances, has very strong bioactivity and is easy to absorb; the enzyme activity of rose plants can be effectively enhanced and the metabolic capability of cells is enhanced; the absorption and transportation conversion ratio of nutrients is improved; the synthesis and accumulation of anthocyanin and flavone are effectively promoted, and the synthesis and accumulation of vitamin C are promoted; the anti-oxidization activity is effectively improved and the nutritional and health-care value is improved; the flowering rate of roses can be effectively improved and the flowering time is prolonged, so that the flowers are large and have a bright color; the ornamental value of the flowers is effectively improved.

The invention discloses water-soluble biological organic fertilizer. The water-soluble biological organic fertilizer is prepared from the following components: chicken manure, potassium fulvate, amino acid, an organic material and a compound flora, wherein the organic material comprises one of starch or brown sugar or combination thereof, and rice bran; the compound flora is prepared from the following components: bacillus subtilis, pediococcus pentosaceus, aspergillus niger, bacillus mucilaginosus, rhizopus oryzae, pichia farinosa, sphingobacterium multivorum and ferment bacteria. By combining ferment bacterium strains and fermenting the organic material and the chicken manure, the water-soluble biological organic fertilizer has abundant useful microbiological florae; the solubility is improved after sufficient fermentation; the potassium fulvate and the amino acid are added and are mixed and stirred after a fermented product is dried; then a mixture is crushed and is ground and sieved, so that the water solubility is guaranteed. After the water-soluble biological organic fertilizer is applied, nutrients can be supplemented in time and long fertilizer efficiency time is guaranteed; the water-soluble biological organic fertilizer is convenient to use and manpower and material resources are effectively saved.

The invention discloses a compound biological synergist for petrochemical wastewater treatment and a preparation method thereof. The compound biological synergist comprises a complex flora and nutrients, wherein the complex flora is Rhodopseudomonas, Rhodospirillum rubrum, Lactobacillus acidophilus, Pediococcus, Bacillus subtilis, yeast, Acinetobacter, Lampropedia sp. and Thiobacillus denitrificans, and the nutrients are sodium humate, glucose, citric acid and glutamic acid coagulate. The invention relates to the technical field of water treatment. By using the compound biological synergist for petrochemical wastewater treatment and the preparation method thereof, the problems that petrochemical wastewater is susceptible to impact and microorganism poisoning is well solved, By adding high-efficiency bacteria and nutrients, the invention enhances the impact resistance and toxicity resistance of the biochemical system, improves the effluent quality, can improve the load for treating organic pollutants and the stability of microbial structure, improves the ability of the biochemical system to resist toxic and harmful substances, and provides reliable guarantee for further improving the effluent quality.

The invention provides pediococcus pentosaceus and the accession number of the pediococcus pentosaceus is CCTCC M 2019323. Tests prove that the DPPP free radical scavenging rate of an extracellular supernatant of the pediococcus pentosaceus CCTCC M 2019323 is 81.89% and the tyrosinase inhibition rate is 64.82%. The extracellular supernatant of the pediococcus pentosaceus CCTCC M 2019323 is fermented by taking a medicinal plant as a matrix and the fermentation liquor has a good propionibacterium acnes inhibiting effect. Meanwhile, the medicinal plant fermentation liquor can also be applied to preparation of cosmetics, and the prepared plant fermentation liquor product containing the pediococcus pentosaceus CCTCC M 2019323 can effectively remove free radicals, promote skin renewal and reducesynthesis of melanin. The pediococcus pentosaceus has the broad application prospects.

The invention belongs to the technical field of functional food, and particularly relates to a preparation method for active probiotic fermentation sour soybean milk. The soybean milk for fermentation is prepared by uniformly mixing self-made soybean milk and pasteurized milk at the ratio of 8 to 2, performing homogenization treatment, and lactobacillus plantarum and lactococcus lactis are added into the soybean milk for fermentation at a ratio for fermentation. The preparation method comprises the following steps: preparing the self-made soybean milk; preparing the soybean milk for fermentation; performing activation and enlarge cultivation on lactobacillus plantarum and pediococcus pentosaceus; performing fermentation and post-fermentation on sour soybean milk.

The invention discloses a method for separating pediococcus pentosaceus, which comprises the following steps: A, regulating the pH of a prepared MRS (de Man, Rogosa and Sharpe) solid culture medium, and then, placing the MRS solid culture medium in a sterilizing pot for sterilizing, and naturally cooling the MRS solid culture medium; B, mixing a sample to be separated by using sterile double distilled water, whirling the mixture, and then standing the mixture; C, removing to obtain a supernatant fluid, thinning the supernatant fluid, respectively taking three dilution degrees of 10 to the power of -3, 10 to the power of -4 and 10 to the power of -5, respectively mixing the mixture with the prepared liquid MRS solid culture medium, adding the mixture in a glass plate, and shaking a culturing dish, so that the culture medium and a sample mixture are distributed at the bottom of the culture dish; and D, after the culture medium and the sample mixture are solidified, adding the MRS solid culture medium, solidifying the mixture, and then placing the mixture in a biochemical incubator to culture, so that the pediococcus pentosaceus is separated at the bottom of the culture dish. The method is feasible, and is simple and convenient to operate; after the sample to be separated is thinned, a diluent is poured into the plate; after the diluent is solidified, the diluent is poured into the culture medium to form an anaerobic environment; the probability that separated bacteria are cloned into the pediococcus pentosaceus is 100%; the method is more accurate; and the workload is reduced obviously.

The invention provides a preparation method of a forest and grass plant protein source probiotic fermented feed, and relates to the technical field of feeds. The preparation method comprises the following steps: (1) chopping tender branches and leaves, rubbing and drying to obtain a material A; (2) drying and chopping Rumex dapibus herba to obtain a material B; (3) mixing lactobacillus plantarum,pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici, and adding organic acids, short-chain fatty acids, amino acids, enzymes and vitamins to obtaina probiotic stock solution; and (4) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, diluting the probiotic stock solution obtained in the step (3) with water, spraying the diluted probiotic stock solution to the material C, performing uniform stirring, and performing sealed fermentation so as to obtain the forest and grass plant protein source probiotic fermented feed. The method is rich in raw material source and low in production cost, and the prepared feed can improve animal milk quality and milk yield.

The invention discloses a compounded enzyme preparation for improving fertility of a farmland and a preparation method for the compounded enzyme preparation. The compounded enzyme preparation comprises the following ingredients: sucrase, protease, amylase, cellulase, pectinase, xylanase, phosphatase, phytase, urease, dehydrogenase, aryl sulfatase, filamentous yeast, rhizopus oryzae, sticky actinomycetes, bacillus mucilaginosus, pediococcus pentosaceus and a water retention agent. The compounded enzyme preparation is obtained through compounding the above-mentioned diverse bio-enzymes and microbial agents. When the compounded enzyme preparation and an organic fertilizer are compounded and are applied to the farmland, the physicochemical properties of soil can be improved remarkably, the soil is loosened, a hardening phenomenon is improved, a pH value of the soil is increased, the content of organic matters, available nitrogen-phosphorus-potassium and beneficial bacteria in the soil is increased, the fertility of the farmland is improved, and the growth of crops and the improvement on quality of fruits of the crops are prompted.