Gel-type enzyme formaldehyde removing deodorant and preparation method and application thereof
A technology of deodorizing and deodorizing agents and deodorizing agents, applied in the direction of deodorization, separation methods, chemical instruments and methods, etc., can solve the problems of high cost of chemical deodorants, high production costs, secondary pollution, etc.
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Embodiment 1
[0037] A gel-type enzyme formaldehyde-removing, deodorizing and deodorizing agent is prepared by the following method:
[0038] (1) Inoculate Issakuria orientalis into liquid medium A, culture and stand for 72 hours at 37°C to obtain Issakia orientalis culture medium, the total effective number of viable bacteria in the culture medium is 3.6×10 8 a / mL;
[0039] (2) Inoculate lactic acid bacteria paracasei into liquid medium A, shake and culture at 37°C for 36 hours, and the total effective number of viable bacteria in the culture medium for lactic acid bacteria paracasei is 2.3×10 8 a / mL;
[0040] (3) Inoculate Pediococcus pentosaceae in liquid medium B, shake and culture at 37°C for 48 hours to obtain Pediococcus pentosaceae culture solution, the total effective number of viable bacteria in the culture solution is 7.5×108 a / mL;
[0041] (4) Inoculate baker's yeast in liquid medium A, shake and culture at 37°C for 48 hours to obtain baker's yeast culture solution, the total ...
Embodiment 2
[0052] A gel-type enzyme formaldehyde-removing, deodorizing and deodorizing agent is prepared by the following method:
[0053] (1) Inoculate Issaccharomyces orientalis, Lactobacillus paracasei, Pediococcus pentosaceus and baker's yeast into the liquid medium according to the ratio of the number of bacteria 1:1:1:1, and culture it statically at 37°C to make the culture medium The total number of viable bacteria in the 9 cells / mL, the culture solution was centrifuged at 4°C and 3500rpm, and the supernatant was collected;
[0054] (2) Add the supernatant of step (1) to a dialysis membrane with a molecular weight cut-off of 14000, and put it into a phosphate buffer (9.46 g of disodium hydrogen phosphate in water, adjusted to pH 7.0 with potassium dihydrogen phosphate solution , fixed to 1L), dialyzed to remove low-molecular-weight substances at 5°C and magnetic stirrer stirring conditions, recovered the substances in the dialysis membrane, and obtained a liquid deodorizer;
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Embodiment 3
[0062] Gas detection tube detection deodorization effect:
[0063] At room temperature, add 70ppm trimethylamine into a 1L brown glass bottle, and then spray 1ml of the liquid deodorizer prepared in Example 1 or 2 respectively. After 10 minutes, 1 hour, 3 hours, 5 hours, and 24 hours, extract 50ml of the gas in the container, and add an amine detection tube to detect the concentration of the amine gas. The culture medium not inoculated with microorganisms was used as the control group. Compared with the control group, the liquid deodorant obtained by the method of the present invention can completely digest and decompose trimethylamine within 1 hour, and has excellent deodorizing properties.
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