3219 results about "Yeast fungi" patented technology

Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

The invention provided herein relates to vaccines that can be tailored to achieve a desired immune response. Some compositions provided herein are used for preferentially eliciting a humoral immune response while other compositions are useful for preferentially eliciting a cell-mediated response. Combinations of vaccine compositions are also useful for eliciting both types of responses and/or for modulating the type of immune response elicited. The invention also provides methods for eliciting an immune response in an individual by administering the compositions disclosed herein. These immune responses are useful for protecting an individual from various types of diseases, infections, and undesirable conditions.

The present invention relates to feed additives for cats. The invention provides methods for making a biological compositions comprising yeast cells that can improve the immune functions of cats. The invention also relates to methods for manufacturing the biological compositions, and methods of using the biological compositions as feed additives.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

The present invention provides biological fertilizer compositions that comprise yeast cells and garbage. The yeast cells of the invention have an enhanced ability to fix atmospheric nitrogen, decompose phosphorus minerals and compounds, decompose potassium minerals and compounds, decompose complex carbon compounds, overproduce growth factors, overproduce ATP, decompose undesirable chemicals, suppress growth of pathogenic microorganisms, or reduce undesirable odor. The biological fertilizer composition of the invention can replace mineral fertilizers in supplying nitrogen, phosphorus, and potassium to crop plants. Methods of manufacturing biological fertilizer compositions, and methods of uses are also encompassed.

The invention discloses a method for preparing fermented fruit and vegetable juice and application of a product obtained by the method, and the method comprises the following steps: mixing fruits and vegetables to obtain a high-Brix-degree material with the Brix greater than or equal to 30 degrees, adding yeast for first-time fermentation, and adding lactic acid bacteria for secondary synergistic fermentation when the Brix is reduced to below 30 degrees. The fruit and vegetable juice is fermented by use of microorganism for metabolism to produce amino acids, organic acids, alcohols, esters, and other flavor substances to enhance fragrance and enrich the taste. The fermented fruit and vegetable juice prepared by the method can be directly eaten, can be added as a food ingredient into food can also be used as a raw material for preparing a concentrated main agent after blending and concentration. Compared with products on the market in prior art, the preparation method can solve the problem that high-Brix-degree fruit and vegetable juice is difficult to ferment, light in flavor and single in fragrance, and has the advantages of simple process, low cost, convenient transportation, broad market prospects, and the like.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention discloses a recombinant microorganism for preparing dharma diene and protopanoxadiol and a construction method of the recombinant microorganism. The construction method of the recombinant bacteria comprises a step of adding dharma diene synthase, protopanoxadiol synthase and nicotinamide adenine dinucleotide phosphate - cytochrome P450 reductase encoding gene into saccharomyces cerevisiae to obtain recombinant bacteria I. According to the recombinant microorganism for preparing the dharma diene and the protopanoxadiol and the construction method of the recombinant microorganism, by means of homologous recombination, the dharma diene synthase, the protopanoxadiol synthase and the nicotinamide adenine dinucleotide phosphate - the cytochrome P450 reductase encoding gene are all added into the saccharomyces cerevisiae to obtain the initial recombinant bacteria, and the effect that the initial recombinant bacteria can produce trace amount of the dharma diene and trace amount of the protopanoxadiol is discovered; tHMG1 activity of the initial recombinant bacteria is further improved, and therefore intermediate recombinant bacteria are obtained, and by means of the intermediate recombinant bacteria, the yield of the dharma diene and the yield of the protopanoxadiol are significantly increased; the activity of one or two or three of ERG1, ERG9 and ERG20 are improved on the basis of the intermediate recombinant bacteria, and the effect that the recombinant bacteria which can be used to increase the yield of the dharma diene and the yield of the protopanoxadiol are constructed is also discovered. By means of the recombinant microorganism for preparing the dharma diene and the protopanoxadiol and the construction method of the recombinant microorganism, the foundation is laid for artificially synthesizing the dammar diene and the protopanoxadiol.

The invention relates to a compound mushroom flavoring and a preparation method thereof. The main raw materials of the compound mushroom flavoring comprise meadow mushroom powder, straw mushroom powder, sodium glutamate, salt, sugar, maltodextrin, yeast extract, disodium 5'-ribonucleotide and yolk powder. The preparation method of the compound mushroom flavoring comprises the following steps: rinsing, boiling, pulverizing, pulping, homogenizing, concentrating and spray-drying fresh mushrooms, and then, obtaining mushroom powder; adding other raw materials; and carrying out the processes of mixing, granulating, drying and the like to obtain the compound mushroom flavoring. The compound mushroom flavoring has the flavor of the meadow mushroom and the straw mushroom simultaneously by organically mixing the sodium glutamate, the yeast extract, the disodium 5'-ribonucleotide and the mushroom powder, has strong functions of increasing freshness and increasing fragrance and has soft flavor. The cell walls of mushrooms can be broken by the homogenizing action to better release the substances in the cells of the mushrooms, thereby further improving the nutrient value of the product. The sugar is added in the concentration process, thereby further improving and increasing the flavor of the materials. The compound mushroom flavoring is granular and is convenient in use.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention belongs to the technical field of additives, and particularly discloses a mycotoxin purification agent for feed, a preparation method and an application of the agent. The mycotoxin purification agent contains montmorillonite, bentonite, bacillus and fermentation concentrate thereof, inactivated compound lactobacilli and fermentation concentrate thereof, and yeast cell wall polysaccharides. When the mycotoxin purification agent for feed provided by the invention is adopted, mycotoxin can be adsorbed and degraded simultaneously, mycotoxin in the feed is effectively removed, damages of the mycotoxin to animals are reduced, and incidence rate of gastrointestinal diseases and other diseases is reduced. The mycotoxin purification agent has the characteristics of simple preparation method, wide material source, low price and great market application value in the field of feed additives.

The invention discloses a culture feed for piglets, which mainly comprises corn, rice, full-cream puffed soybean, fermented bean pulp, peeled bean pulp, fish meal and the like. Yeast cell extract is added to enhance the immunity of piglets, and compound enzyme preparations and probiotics are used instead of high copper and high zinc, thereby lowering the environmental pollution, enhancing the digestion and absorption of the feed, and maintaining normal flora of the intestinal tract. Full organic trace elements are used instead of inorganic trace elements, thereby lowering the oxidization of inorganic salts on the feed and organisms. The invention also discloses a preparation method of the culture feed, which effectively reserves beneficial ingredients and discards harmful ingredients through the granulation process, thereby effectively reserving the nutritive value of the feed.

The invention relates to a fermented liquid feed for pig and poultry as well as a preparation method and an application thereof, belonging to the field of biotechnology, in particular to a liquid feed produced by the fermentation of a plurality of beneficial microbes and Chinese medicinal herbs as well as a preparation method and an application thereof. The preparation method has simple process and comprises the following steps: after primary slant culture and secondary solution culture, lactobacillus, fermentation lactobacillus, bacillus subtilis, natto bacillus and saccharomyces cerevisiae are put into a solid fermenting tank with a fermentation medium containing astragalus root, Na2HPO4, nitrogen source and carbon according to weight proportion. The fermented liquid feed adopting the method has high quality, thicker mellow flavor and short fermenting time, contains protein, polypeptide, small peptide, amino acid, astragalus polyose and a plurality of beneficial microbes and is applicable to preparing various perfect compound feeds for pig and poultry.

The invention discloses a genetically engineered bacteria used for producing stevia glycosyltransferase UGT76G1; a UGT76G1 coding gene is inserted between the restriction enzyme cutting sites EcoRI and XhoI of a PYes2 carrier, so as to construct a recombinant plasmid, and then the recombinant plasmid is introduced to expression host Saccharomyces cerevisiae YPH499 to obtain the engineered bacteria; and the coding gene of the UGT76G1 is GenBank, No. GenBank: AY345974.1, and the gene sequence of the coding gene is named as UGT (Udp Glucuronyl Transferase). The invention also discloses a construction method of the genetically engineered bacteria and the application of the bacteria to the production of rebaudioside A. According to the invention, under the condition that expensive UDPG (Uridine Diphosphate Glucose) is not added, cheap carbon source glucose is used as a substrate, the metabolic pathway of UDPG in the yeast is regulated, and then the rebaudioside A is produced from St glycosides through whole cell catalysis.

The invention provides a feed for improving the disease resistance of a grass carp. The feed is characterized by comprising soybean cake, wheat meal, shell meal, fish meal, table salt, rice bran, fish oil, betaine, traditional Chinese medicine feed premix additive, zinc-containing polypeptide, zinc-containing microelement additive, vitamin mixture additive at least containing vitamin C, vitamin E and inositol, yeast and amino acid mixture additive at least containing threonine. The feed provided by the invention can improve the immunity of the organism of the grass carp and protect the grass carp from getting sick; the application of antibacterial chemical medicines and anti-virus chemical medicines is reduced to a certain degree, the survival rate of the grass carp is increased, and the activity of lysozyme and superoxide dismutase in the grass carp is improved; and moreover, the feed ensures little drug residue and low toxicity, and does not influence the quality of the grass carp or harm the human health.

The invention relates to a method for preparing a mixed-type nucleotide feed additive from yeast cells and applications of the feed additive, which belong to the technical field of the preparation of nucleotide feed additives from yeast cells. The method comprises the steps: using yeast cells as raw materials and combining a homogenization method and a salt method to break yeast cell walls ; separating the yeast cell walls, protein and other insoluble substances by centrifugation and fractional isoelectric crystallization; specially collecting RNA precipitates at special isoelectric points to obtain wet and crude nucleic acid with the purity of 70 percent; dissolving the wet and crude nucleic acid in water, using high-activity phosphodiesterase from malt roots to carry out a high-efficiency directional enzymatic reaction; directly spraying, drying and crushing the solution after the enzymatic reaction to obtain the mixed-type nucleotide feed additive product comprising the protein content lower than 10 percent and four monosomic nucleotides (AMP, GMP, CMP and UMP) larger than 60 percent. After the feed additive is applied to animal feed, the intestinal absorption function of animals can be notably improved, the growth of the animals is stimulated, and the immune function, the antiviral capability and the anti-stress capability of the animals are improved.

The invention discloses a method of using waste bear yeast to simultaneously prepare zymosan, trehalose and yeast extract. The method comprises steps of debittering and impurities removing of the waste bear yeast, autolysis, enzymatic hydrolysis and inactivation of the waste bear yeast, separation and drying of the zymosan, membrane separation, concentration, crystallization and drying of the trehalose, concentration and drying of the yeast extract, etc. The method has the characteristics of high raw material utilization rate, good quality, low cost and easy industrialization, realizes that three products of the zymosan, the trehalose and the yeast extract are simultaneously produced from the same industrial waste, the waste bear yeast, improves the utilization rate of industrial wastes and achieves the target of maximal waste resource recovery.

The invention provides a method for producing an organic fertilizer from municipal dry branch/fallen leaf waste and application thereof. The method comprises the following steps: collecting branches, fallen leaves and other municipal greening wastes, and mixing Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Trichoderma reesei to obtain a composite microbial inoculant; adding bran coat, chaff powder or peanut hull meal, the composite microbial inoculant, urea and potassium dihydrogen phosphate, thoroughly mixing, piling into a 1.5-2m-high cone, and controlling the water content at 60-70%; carrying out hide lifting on the pile in the composting process, and fermenting for 20-30 days; and carrying out aerobic and anaerobic fermentation on the dry branch/fallen leaf waste by using the microbial inoculant to obtain the organic fertilizer. The preparation technique is simple; and the prepared organic fertilizer can improve the soil and reduce the environmental pollution caused by the waste.

The invention discloses a microbial deodorizing inoculant which is used in refuse sites, public toilets and livestock/poultry cultivation farms. The microbial deodorizing inoculant contains Candida utilis of which the collection number is ACCC 20060, Lactobacillus plantarum of which the collection number is ACCC 11016, lactic streptococci of which the collection number is ACCC 10653, cerevisiae fermentum of which the collection number is ACCC 21135, Streptococcus thermophilus of which the collection number is ACCC 10651, Bacillus licheniformis of which the collection number is ACCC 01870, and Bacillus natto of which the collection number is ACCC 19833. The microbial deodorizing inoculant absorbs and degrades generated malodorous gas hydrogen sulfide and ammonia gas, eliminates the malodor of the garbage and garbage leachate, inhibits the putrefaction bacteria from growth, basically prevents the malodorous gas from generation, and forms a disease-spread-free refuse piling environment capable of eliminating the malodor.

The present invention relates to fungal Δ12 fatty acid desaturases that are able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific ω-3 and ω-6 fatty acids by over-expression of the Δ12 fatty acid desaturases are also described herein.