119 results about "Two stage fermentation" patented technology

Methods and systems for the production of one or more lipid products from a gaseous substrate using a two stage fermentation process are provided. The method comprises providing a gaseous substrate comprising CO₂ and H₂ to a primary bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce an acid such as acetate. The acid, e.g. acetate from the primary bioreactor is then provided to a secondary bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.

The invention discloses a method for simultaneously preparing 1,3-propylene glycol and 2,3-butylene glycol from glycerol by using microbe employing prior-stage anaerobic and later-stage aerobic two-stage fermentation process, which belongs to the field of the bio-chemical engineering technology. In the fermentation process, the seed liquid is added in the primary fermentation medium containing industrial glycerol or glycerol fermentation liquid, after 3-5 hours, the mixed liquid of glycerol and glucose is added in the form of the flow. The product concentrations of 1,3-propylene glycol and 2,3-butylene glycol are controlled by adjusting the air flow in the two-stage fermentation process and controlling the fermentation temperature. When the fermentation process is over, 1,3-propylene glycol and 2,3-butylene glycol are separated and purified by desalting, distilling, and vacuum rectification. The preparing method can improve the fermentation concentration and the yield of 1,3-propylene glycol and the production intensity, at the same time, 2,3-butylene glycol with higher concentration can be obtained with high raw material availability and low cost.

This invention relates to a microbial flocculating agent. The invention isolates a high-yield bioflocculation microorganism-Paenibacilluspolymyxa GA1 CCTCC M 206017, and utilizes bean dregs as the substituted culture medium to synthesize the flocculating agent. The culture process comprises: processing the bean dregs at pH11.0 by boiling at high temperature and pressure, adjusting the pH to pH6.0-8.0, inoculating with 0.5-2% seed solution, applying two-stage fermentation process, wherein: at the early state of 24 hours, the culture temperature is 30deg C and the shaker speed is 150r/min; and at the late stage of 32 hours, the culture temperature is 25deg C and the shaker speed is 100r/min. By applying the two-stage fermentation process, the bacteria can make use of nutrients to synthesize the flocculating agent at a maximu rate, with the yield increaed to 7.86g/L and culture cycle decreased to 56h. By using the bean dregs and the two-stage fermentation process, it can lower the cost for producing the microbial flocculating agent, improve the yield, and realize the massive industrial production of microbial flocculating agent.

The invention relates to a method for preparing a germinated brown rice ferment drink. Germinated brown rice is processed in an Aspergillus oryzae and Rhizopus oryzae mixed culture manner, is decomposed by using the enzyme system complementarity, and is inoculated with lactic acid bacteria to carry out fermentation. The prepared germinated brown rice ferment drink is rich in organic acids, variousfree amino acids, soluble dietary fibers, and active components which are gamma-aminobutyric acid and SOD, and has the advantages of sour-sweet mouthfeel, convenience in drinking, no additives, excellent nutrition and healthcare effects, great improvement of the raw material utilization rate in a two-stage fermentation process, and great solving of resource wastes of existing brown rice having bad mouthfeel.

The invention discloses a method for constructing genetic engineering bacterial strain Escherichia coli JM125 producing boletic acid and method for producing acid. The constructing process comprises inactivation or out-knocking enzymes in facultative microbiology which has abilities of reduction boletic acid and degrading acetonic acid, and exceeding expressing NAD regeneration. The following steps of two-stage fermentation experiment using constructed Escherichia coli are (1) activating bacillus coli is transferred to enrichment medium and cultured to improve biomass. (2) The treated bacterium liquid membrane obtained by aerobic cultivation is transferred into LB culture medium which contains 20 to 100g/L amylaceum and certain concentration carbonates to produce fumaric acid by anaerobic fermentation.

The invention relates to a method for the fermentation production of succinic acid by the circulating utilization of recombinant Bacillus coli cells, which is characterized by comprising the following steps: adopting the two-stage fermentation mode of first aerobic culture of Bacillus coli and later anaerobic acid production to produce succinic acid by utilizing Bacillus coli for producing succinic acid; when the two-stage fermentation is finished, utilizing metabolites (succinic acid, acetic acid, pyruvic acid and the like) produced in the fermentation process to aerobically induce cells; aseptically collecting Bacillus coli cells, and anaerobically biotransforming and synthesizing the succinic acid in a fresh culture medium; after finishing the anaerobic biotransformation and synthesis,aerobically inducing again, and recovering the cells to continue to biotransform and synthesize the succinic acid in the fresh culture medium, and achieving the purpose of circulatingly utilizing thecells to biotransforme and synthesize the succinic acid. The technical scheme of the invention solves the problems that the yield and production intensity of the succinic acid is on the low side in the whole process of two-stage fermentation due to the consumption of a great deal of carbon sources and time in the process of aerobic Bacillus coli culture.

The invention discloses a preparation method of Se-enriched nutrient corn beverage, which is characterized by comprising the following steps of: using fresh sweet corn as raw material, conducting controlled hydrolyzing on fresh sweet corn steep liquor starch liquid by using amylase and maltogenic amylase, then using Lactobacillus acidophilus and Se-enriched beer yeast to carry two-stage fermentation on corn liquid, and obtaining a novel Se-enriched natural corn beverage product with rich health care functional factors and good taste.

The invention belongs to the microorganism field, particularly relates to a corayceps militaris polysaccharide two-stage fermentation synthesis process; the process comprises the following steps: corayceps militaris strains are vaccinated on a potato slant culture medium, are cultured at the constant temperature and then are put in a conical flash containing a seed culture medium for shaking culture; then the corayceps militaris strains are put in a fermentation tank containing a fermentation medium, the fermentation process is as follows: the corayceps militaris is cultured at constant temperature for four to five days in ventilating and stirring condition, which is mainly used for providing a culture condition suitable for growing of corayceps militaris thallus so that the corayceps militaris grows rapidly; then the corayceps militaris is cultured for two to three days at the constant temperature in the condition of ventilation and slightly stirring, a culture solution is centrifuged, and ethanol in two times of volume of is added into an obtained liquid supernatant which is mixed and put up for the night at the temperature of 4 DEG C; and precipitates obtained through secondary centrifugation is dried so as to obtain the corayceps militaris. The process is reasonable in design and simple in operation, is suitable for large-scale industrial production and can solve the situation of drug source shortage and has obvious benefits.

The invention belongs to the field of foods, and particularly relates to a low-salt quick fermentation technology of Pixian thick broad-bean sauce. The technology comprises the following steps of (1) making low-salt fermented hot pepper blanks; (2) making sweet fermented broad beans; and (3) making the low-salt Pixian thick broad-bean sauce: mixing the low-salt fermented hot pepper blanks with the fermented broad beans in a ratio of the low-salt fermented hot pepper blanks to the sweet fermented broad beans being 9 to 1 to obtain a low-salt sauce, putting the low-salt sauce in a sealed fermenting tank or a sealed fermenting jar, adopting a two-stage fermentation technology comprising stirring the sauce, drying the sauce in the sun and allowing the sauce to stand at night for natural fermentation to obtain the low-salt Pixian thick broad-bean sauce, and then subjecting the low-salt Pixian thick broad-bean sauce to a pasteurization technology so as to obtain the finished product of the low-salt Pixian thick broad-bean sauce. According to the technology disclosed by the invention, pre-fermentation is performed, so that the fermentation flavor of the hot peppers can be promoted, and the salinity can also be reduced; under the effects of acid and salt, the growth of putrefying bacteria can be restrained, and pollution caused by infectious microbes in subsequent processes can be avoided; in addition, dried hot peppers and fresh hot peppers are adopted, so that the occupied volume of salting ponds of enterprises can be reduced, and the cost of the enterprises for storing the hot peppers can be reduced; and the fresh hot peppers are added, so that the table salt in the high-salt hot pepper blanks can be diluted, the flavor of the fresh hot peppers can also be increased, and the mouth feel of the sauce can be enriched.

A two-stage fermenting process for preparing composite biologic flocculant includes such steps as physically and chemically pretreating cellulose, preparing culture liquid, adding inorganic salt, regulating pH value, sterilizing, adding cellulose gradating bacteria to convert cellulose to glucose and cellodiase, and adding flocculant generating bacteria.

The present invention provides a process for the production of a microbial oil comprising culturing a micro-organism in a two stage fermentation process where, in a last stage that precedes the end of fermentation, the carbon source is: consumed by the micro-organisms at a rate greater than it is added to the medium; added at a rate ≦0.30 M carbon/kg medium; or is rate limiting on the growth of the micro-organism. The micro-organisms thus have the carbon source restricted so that they preferentially metabolise fats or lipids other than arachidonic acid (ARA), so increasing the proportion of ARA in the cells. A microbial oil is then recovered from the micro-organism, using hexane as a solvent, that has at least 50% ARA and at least 90% triglycerides.

The invention discloses a two-stage anaerobic treatment system for cassava alcohol wastewater. The system comprises a primary heat exchanger, a high temperature anaerobic fermentation tank, a primary drum screen, a secondary drum screen, a secondary heat exchanger, a sedimentation pool, an air floatation pool, an adjustment pool and a medium temperature UASB reactor. Wastewater goes by the primary heat exchanger for cooling, enters the high temperature anaerobic fermentation tank for high temperature anaerobic fermentation, goes by the two-stage drum screen, the secondary heat exchanger, the sedimentation pool and the air floatation pool for removing suspending matters and partial COD, and enters the UASB reactor for medium temperature anaerobic fermentation. The system has the following advantages: biogas generated in the two-stage fermentation is collected and used, and wastewater enters a subsequent aerobic segment for continuous treatment; and the whole treatment process flow is concise, the management is convenient, the energy consumption of the production and the operation is low, the treatment efficiency is high, and sludge has a less output and is easy to dispose.

The invention relates to a method for producing cellulase, and belongs to the field of biology technology. The invention is characterized in that two stage fermentation of golden mushroom bran containing lactic acid bacteria and composite mould is used for producing cellulase, and the method has the advantages of wide enzyme raw material source, low cost, complete enzyme system, good stability and high efficiency; the method not only can be used for large scale industrial production, but also can alleviate environment pollution problem. Average enzyme activity of the prepared enzyme is 572 U/g, and not only can be used for producing fuel ethanol, but also can be used for textile, foodstuff, forage and chemical industry, etc.

The invention provides a biologic deodorant as well as a preparation method and an application thereof. The biologic deodorant is a biologic fermentation liquid of which a microorganism strain is combined with one or more of yeast strains or one or two of bacteria into a mixed bacterium, wherein the yeast strains comprise candida Antarctica lipase and candida rugosa; the bacteria comprise bacillus licheniformis and bacillus subtilis; after microorganism strains are subjected to strain domesticating by using tea seed cake dregs as a sole nitrogen source, a two-stage fermentation process is adopted for fermentation, finally the microorganism strains in a fermentation bacterial liquid are in the dormant state, and the final biologic deodorant is obtained; when the biologic deodorant is used, after water is added and the biologic deodorant is in contact with air, the microorganism strains can continuously grow and generate more enzymes. By adopting the biologic deodorant provided by the invention, organic matters which can generate odor can be fundamentally removed, the degree that a pipeline is blocked by organic matters is reduced or eliminated, and the biologic deodorant can be also used for washing oil-containing or/and protein-containing or/and starch-containing dirt, does not damage any pipeline in a sewer, and is environmental-friendly.

The embodiments of the invention disclose a method for treating landscaping solid organic waste by biomass carbonization and a two-stage fermentation combined process. The method includes the following steps: separating branches, trunks and roots from landscaping solid organic waste to obtain a mixed material of the branches, trunks and roots and remaining materials; crushing the mixed material and carbonizing the mixed material to obtain wood tar, pyroligneous liquor containing condensed water, biomass charcoal and combustible gas; crushing the remaining materials and evenly mixing the remaining materials with livestock manure, water, the wood tar and the pyroligneous liquor containing condensed water to obtain a fermentation material; and carrying out the two-stage fermentation combinedprocess treatment on the fermentation material to obtain basic organic fertilizer. The method firstly carries out classification and then carries out treatment by combination of multiple processes, can make good use of complementary advantages of the multiple processes to prepare a variety of resource utilization products, realizes the balance of energy supply and demand, effectively shortens fermentation time, and improves fermentation efficiency and final product quality.

The present invention relates to feed additive containing microorganism. It is made up by using trichodermin, aspergillus, actinomycetes, bacillus and saccharomyces cerevisiae Li1012 through the processes of pure strain amplification culture, liquid fermentation culture and solid two-stage fermentation culture.

The invention relates to the field of fermentation production processes, and discloses a chlortetracycline fermentation production method using refined protein powder. According to the present invention, Streptomyces aureus is adopted as a starting strain, and pure culture and two-stage fermentation are performed to produce chlortetracycline; with the application of the 100% refined protein powder to replace the soybean cake powder, the chemical titer is increased by 2.3% compared to the application of the soybean cake powder, and the yield is increased by 1.8% compared to the unimproved method, such that the yield is effectively increased; and the raw material cost input per tank can be reduced by about 540 yuan after the cost accounting, the effect is good, the fermentation production level is stably increased, and the theoretical and applied basis is provided for the industrial production of the antibiotics and the scientific research.

A naturally caffeinated coffee fruit alcoholic beverage and method for producing the same are disclosed and described. In some embodiments, this beverage may comprise natural alcohol in a range of about 1% to about 11% ABV from at least microbial fermentation of dissolved sugar; naturally extracted caffeine from maceration, freezing, thawing, and microbial fermentation of coffee fruit flesh; natural coffee fruit flavor from maceration, freezing, thawing, and microbial fermentation of coffee fruit flesh; natural ginger flavor from maceration, freezing, thawing, and microbial fermentation of ginger; dissolved gas at about 1.75 to 3.30 volumes of the dissolved gas; and water. The natural alcohol, the natural caffeine, the natural coffee fruit flavor, the natural ginger flavor, and the dissolved gas may each be dispersed within the water. The method may comprise use of a mixed microbial culture and two stage fermentation process.

The invention belongs to the technical field of biological engineering, and relates to a gene engineering bacterium strain for producing succinic acid, and a method for producing succinic acid by fermentation by using the same. The classification designation of the gene engineering bacterium strain for producing succinic acid is Escherichia coli BA305, and the preservation number is CCTCC NO:M2012102. The construction process mainly comprises the following steps: inactivating or knocking out phosphoenolpyruvic acid carboxylase gene and ptsG gene in a phosphate translocation system; and overexpressing the phosphoenolpyruvic acid carboxylase so that the recombinant colibacillus can efficiently utilize glucose, xylose, arabinose, levulose and other monosaccharides and efficiently utilize mixed saccharides in various proportions and cellulose hydrolysate, thereby greatly enhancing the synthesis efficiency of the succinic acid. The fermentation method adopts a two-stage fermentation mode: the aerobic stage can enhance the biomass, and the anaerobic stage is used for fermentation to produce the acid.

The invention discloses a recombinant plasmid for producing succinic acid and a succinic acid high-yield genetic engineering bacterium. The invention also discloses a method for preparing the recombinant plasmid and the genetic engineering bacterium. The invention simultaneously discloses application of the recombinant plasmid and the genetic engineering bacterium to the preparation of biological products for fermentation production of the succinic acid in the genetic engineering. The genetic engineering bacterium which is established by the recombinant plasmid has high stability, and improves the growth rate compared with the original strain BL21 (DE3); and the yield of the succinic acid is greatly improved under the condition of addition of revulsant with different concentrations, namely lactose or IPTG and two-stage fermentation of completely anaerobic or aerobic accumulation of biomasses and oxygen-free acid fermentation.

The invention discloses a rapid production method of lactic acid bacteria fermented soya beans capable of dissolving fibrous protein. According to the rapid production method, two-stage solid-state fermentation is adopted to produce functional fermented soya beans. The rapid production method comprises following steps: soybean is selected, and impurities are removed; soybean is immersed in warm water at a ratio of 1:2-3 for 6 to 9h, wherein the temperature of the warm water ranges from 25 to 30 DEG C; steaming is carried out for 8 to 15min at 110 to 121 DEG C, after cooling, an obtained product is inoculated with 3 to 5% (V/W) of SYL-1 Bacillus subtilis; fermentation is carried out for 42 to 54h at 26 to 32 DEG C so as to obtain a fermented soya beans semi-finished product, and the fermented soya beans semi-finished product is inoculated with 3 to 5%(V/W) of SYL-2 Lactobacillus brevis, is stirred to be uniform, and is subjected to fermentation for 42 to 54h at 32 to 37 DEG C; after fermentation, an obtained product is subjected to 72h of post-fermentation in a refrigerator at 4 DEG C so as to obtain a finished product. According to the rapid production method, bioengineering technology is adopted; two-stage fermentation, including bacillus subtilis primary fermentation and lactic acid bacteria post-fermentation, is carried out via combination with traditional fermentation method; flavor components such as fermented soya beans ester substances are increased; nutritional ingredients such as peptone, polypeptides, and amino acids are increased; functional ingredients such as douchi fibrinolytic enzyme, oligosaccharides, organic acids, and lactic acid bacteria are increased; the lactic acid bacteria fermented soya beans is capable of adjusting intestinal flora balance, dissolving fibrous protein, treating constipation, preventing oxidation, and preventing cardiovascular diseases.

A process for preparing alcohol from the saccharomyces cerevisiae 1912 which can resist high-concentration alcohol and sugar includes such steps as culturing while introducing aseptic air, and fermenting while introducing aseptic air. Its advantages are high conversion rate and output rate, and low cost.

The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO or CO₂ and H₂ or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.