Method for producing cellulase

A cellulase and production method technology, applied in the biological field, can solve the problems of low enzyme production efficiency, low enzyme production efficiency, incomplete enzyme system, etc., and achieve the effects of reduced enzyme production costs, wide sources, and improved efficiency

Active Publication Date: 2014-04-23
明博医药技术开发(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are fundamental defects in the traditional solid fermentation method: (1) natural cellulose raw materials such as corn and other crop stalks are water-insoluble, and cannot be directly absorbed by the fermentation cells during the cultivation process and enter the cells to induce cellulase. Synthetic; (2) cellulase inducer can only be ferment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Trichoderma viride in the compound mold liquid: Aspergillus niger=1.5.

[0025] (2) The inoculation amount of the lactic acid bacteria liquid in the edible fungus chaff was 0.1% of the dry matter. After 5 days of anaerobic fermentation, the compound mold liquid was inoculated at 1% of the dry matter, cultured in ventilation, and fermented at 30°C for 7 days.

Embodiment 2

[0027] (1) Trichoderma viride in the composite mold liquid: Aspergillus niger=1.

[0028] (2) The inoculum amount of the lactic acid bacteria liquid in the edible fungus chaff is 0.2% of the dry matter. After 4 days of anaerobic fermentation, the compound mold liquid is inoculated at 1.5% of the dry matter, cultured in ventilation, and fermented at 30°C for 6 days.

Embodiment 3

[0030] (1) Trichoderma viride in the compound mold liquid: Aspergillus niger=0.8.

[0031] (2) The inoculation amount of the lactic acid bacteria liquid in the edible fungus chaff is 0.3% of the dry matter. After 3 days of anaerobic fermentation, the compound mold liquid is inoculated at 2% of the dry matter, cultured in ventilation, and fermented at 30°C for 5 days.

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PUM

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Abstract

The invention relates to a method for producing cellulase, and belongs to the field of biology technology. The invention is characterized in that two stage fermentation of golden mushroom bran containing lactic acid bacteria and composite mould is used for producing cellulase, and the method has the advantages of wide enzyme raw material source, low cost, complete enzyme system, good stability and high efficiency; the method not only can be used for large scale industrial production, but also can alleviate environment pollution problem. Average enzyme activity of the prepared enzyme is 572 U/g, and not only can be used for producing fuel ethanol, but also can be used for textile, foodstuff, forage and chemical industry, etc.

Description

technical field [0001] The invention relates to a production method of cellulase, which belongs to the field of biotechnology. Background technique [0002] As fossil fuels become scarce and depleted, energy is a common problem facing mankind. Finding new energy is related to the sustainable development of economy and even the survival of human beings. Cellulose, unlike fossil fuels, is a renewable resource. Photosynthesis on the earth can produce more than 10 billion tons of plant dry matter every year, more than half of which is cellulose and hemicellulose. Cellulose is a polysaccharide that can be decomposed to produce disaccharides or glucose under the catalytic conditions of cellulase, and then fermented to produce energy substances such as ethanol. [0003] It is a pity that the utilization of cellulose has not yet been fully developed, resulting in a huge waste of resources. In order to make better use of cellulose, more and more foreign scholars have begun to pay...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/885C12R1/685
CPCC12N9/2437
Inventor 聂向红马明刘付燕
Owner 明博医药技术开发(上海)有限公司
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