32results about How to "Improve biological activity" patented technology

The invention discloses a production method of distiller's grain fermentation liquid fertilizer specially applied to roxburgh rose. The distiller's grain fermentation liquid fertilizer is prepared bythe step of evenly mixing 50 to 80 parts of distiller's grain fermentation extracting solution, 0.5 to 1 part of microelement, 5 to 10 parts of seaweed meal, 5 to 10 parts of natural active organism,2 to 5 parts of potassium bicarbonate, 2 to 5 parts of natural active organism and 2 to 5 parts of natural surface active agent. A preparation method of the distiller's grain fermentation extracting solution comprises the steps: evenly stirring 50 to 80 parts of distiller's grain, 50 to 150 parts of rice washing water, 1 to 2 parts of EM microflora fermentation liquid and 0.5 to 1 part of sugar, performing composting fermentation at the room temperature to obtain a distiller's grain fermentation material, stirring and extracting through a sodium carbonate water solution, filtering and performing evaporation and condensation on obtained filtrate until the density is 1.1 to 1.2 to obtain distiller's grain fermentation extracting solution. The distiller's grain fermentation liquid fertilizerdisclosed by the invention has a good use effect, meanwhile can effectively prevent pest and disease damage to the roxburgh rose and is high-efficiency environment-friendly liquid fertilizer.

This invention belongs to the field of cosmetic raw material preparation technology, specifically relating to a method for preparing a low-odor Xinjiang Lithospermum extract, its product, and its application. The method includes: raw material pretreatment: drying and pulverizing Xinjiang Lithospermum raw material to obtain pretreated material; first-stage extraction: loading the pretreated material into a supercritical fluid extraction device, adding a composite entrainer for extraction, and collecting the first fraction in a first separator; second-stage extraction: collecting a second fraction rich in naphthoquinone components in a second separator; and post-treatment: concentrating the second fraction and removing the alcohol solvent to obtain the low-odor Xinjiang Lithospermum extract. This invention can separate and discard most of the odor-causing volatile components in the raw material under mild conditions in the first stage, and then efficiently extract the core active substances of naphthoquinone in the second stage, achieving a significant reduction in the odor of the extract and a significant improvement in its sensory quality.

The application provides a method and device for treating wastewater by combining a suspended filler floating bed and a soil infiltration system, and the method comprises the following steps: S1, breeding wastewater is treated by the suspended filler floating bed after passing through a hydrolysis tank, and effluent is treated in a sedimentation tank, and wastewater of the suspended filler floating bed is returned to the suspended filler floating bed for secondary treatment; S2, wastewater of the sedimentation tank is returned to the hydrolysis tank; and S3, effluent of the sedimentation tank is introduced into a soil underground infiltration system. The system provided by the application is beneficial to further strengthening the effects of denitrification, phosphorus removal and removal of suspended solids, and solves the problems of low dissolved oxygen content of influent and wastewater in the system, which is not conducive to the growth of nitrifying bacteria, and low effective volume utilization rate. In the use process, the filler does not need to be replaced, and mechanical aeration equipment does not need to be added, and the system has the advantages of low investment cost, no need to add chemical agents for treatment, obvious effect, not easy to breed mosquitoes, simple operation and management, and beautification of the environment, and has remarkable ecological and economic benefits for promoting deep purification of breeding wastewater.

The application discloses a kind of fulvic acid hydrogen peptide biological stimulant hormone fertilizer and preparation method thereof, belong to fertilizer technical field.Fertilizer is by 15~30 parts of fulvic acid, 5~12 parts of peony seed meal small molecule peptide, 20~35 parts of potassium dihydrogen phosphate and other components compound, water is made up to 100 parts, and auxiliary agent is emulsifier and chelating agent compound.Preparation first by dissolving fulvic acid, potassium phosphate in water and obtaining mixed solution, then adding small molecule peptide and auxiliary agent and stirring until the system is stable;Small molecule peptide is prepared by peony seed meal defatted powder crushing and enzymolysis, solves the problem of traditional fertilizer nutrient antagonism, unstable system, and the utilization of peony seed meal also improves resource utilization.Tomato, cucumber seedling test shows that it can significantly improve the growth index of crop root system and aboveground part, and the root-promoting and seedling-strengthening effect is better than that of similar fertilizer on the market, and the preparation process is simple, easy to industrialization, and has good agricultural application prospect.

This invention discloses a forest-grown Panax notoginseng extract, an oral liquid, its preparation method, and its application, belonging to the field of Panax notoginseng processing technology. The preparation method includes the following steps: (1) fresh forest-grown Panax notoginseng is crushed with water to obtain a Panax notoginseng suspension; (2) a complex enzyme is added to the Panax notoginseng suspension for enzymatic hydrolysis to obtain an enzymatic hydrolysate; (3) the enzyme inactivation of the hydrolysate, solid-liquid separation, and concentration of the clear liquid are obtained; the complex enzyme is cellulase, xylanase, and flavor protease, and the mass ratio of cellulase, xylanase, and flavor protease is 10-20:3-7:2-6. The Panax notoginseng extract prepared by enzymatic hydrolysis using forest-grown Panax notoginseng as raw material has the effects of assisting in lowering blood pressure and enhancing immunity. Compared with conventional Panax notoginseng extracts, this invention has a better effect in assisting in lowering blood pressure.

This application discloses the use of itaconic acid derivatives in the preparation of drugs for treating candidemia, belonging to the field of biomedical technology. The itaconic acid derivatives proposed in this application are dimethyl itaconic acid and / or 4-octyl itaconic acid. Dimethyl itaconic acid exhibits a dose-dependent therapeutic effect in model mice, with an optimal dose of 1200 mg / kg improving survival rate and reducing kidney damage; 4-octyl itaconic acid can enhance the anti-Candida albicans ability of macrophages and regulate the expression of inflammatory factors. The application of the itaconic acid derivatives proposed in this application in the treatment of candidemia works through immunomodulation, circumventing fungal resistance. Furthermore, the drugs are low in toxicity, have good biocompatibility, and use a universal experimental system, providing a new approach for the clinical treatment of candidemia with broad application prospects. This application also protects pharmaceutical compositions for treating candidemia with dimethyl itaconic acid and / or 4-octyl itaconic acid as active ingredients.

The application relates to a soluble silk fibroin pellet quick-freezing, separating and collecting device. The device comprises a low-temperature treatment tank, an external heat preservation tank, a feeding assembly, a separating assembly and an electric control system. The low-temperature treatment tank is nested in the external heat preservation tank and is adiabatic; the feeding assembly is arranged between a top cover plate and an adiabatic plate and comprises a material feeding pipe, a liquid nitrogen feeding pipe and a recovery pipe; a liquid drop pipe is arranged in the material pipe and is provided with a heating device arranged inside the liquid drop pipe to prevent freezing; liquid drops fall through the adiabatic plate into liquid nitrogen to be quickly frozen into small balls. The parameters of a stirring paddle can be adjusted to control the particle diameter, and a peristaltic pump and the specification of the drop pipe are used to control the size. The quick-frozen mixture flows into a separating tank through a low-temperature electromagnetic valve, small balls are intercepted by a screen, and liquid nitrogen is recycled. The electric control system is used for regulating and controlling stirring, the electromagnetic valve and heating power. The device can ensure resolubility through low shear quick freezing, the quick-frozen mixture can be freeze-dried in less than 24 hours due to the high specific surface area of the small balls, the cost is reduced through liquid nitrogen circulation, and the flux and particle diameter can be controlled through multi-parameter adjustment, so that the device is suitable for the preparation of silk fibroin and composite materials.

This invention discloses a highly efficient oxygen removal reagent and its application in the deoxygenation reconstruction of carbon resource molecular skeletons, comprising a pivot reagent, a phosphine reagent, an azo reagent, a reducing agent, an acid reagent, a base reagent, a photosensitizer, and an organic solvent; wherein, X in the pivot reagent includes a hydrazine group or a sulfone group; PG is a protecting group; LG is a leaving group; by using natural oxygen-containing functional groups such as alcohols, aldehydes, and ketones as direct coupling precursors, an integrated in-situ activation-deoxygenation coupling process is achieved, significantly reducing reaction steps.

This invention discloses a lutein composition for relieving eye fatigue and its preparation method, belonging to the field of functional foods. The process involves: reacting phycocyanin and kelp polyphenols to obtain modified phycocyanin; reacting fucoidan and β-glucuronidase to obtain modified fucose; reacting fish collagen peptides and zinc chloride to obtain collagen peptide-zinc chelate; reacting lutein silica cage complex and modified phycocyanin to obtain a first complex; reacting the first complex and modified fucose to obtain a second complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil to obtain a lutein complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil, then adding trehalose to obtain the lutein complex; and finally mixing the lutein complex with maltodextrin to obtain the lutein composition.

This invention belongs to the field of food processing technology, specifically relating to a low molecular weight *Hydrangea hygroscopica* polysaccharide microsphere for relieving fatigue, its preparation method, and its application. The preparation method includes the following steps: (1) extraction of crude polysaccharide from *Hydrangea hygroscopica*; (2) degradation; (3) encapsulation: the low molecular weight *Hydrangea hygroscopica* polysaccharide obtained in step (2) is dripped into CaCl2 solution through electrospray treatment to form a gel core, and the gel core is encapsulated to obtain the low molecular weight *Hydrangea hygroscopica* polysaccharide microspheres. The beneficial effects of this invention are: this invention efficiently extracts *Hydrangea hygroscopica* polysaccharide through steam explosion-pulsed electric field synergy, accurately prepares 10-20 kDa high-activity low molecular weight fragments through compound enzymatic hydrolysis-ultrasound-ultrafiltration, and finally constructs colon-targeting microspheres using electrospray-layer-by-layer self-assembly. These microspheres can protect the active ingredients and allow them to reach the colon for release, significantly improving the bioavailability and anti-fatigue effect of the polysaccharide.

The invention relates to the technical field of biological medicine, and particularly discloses a preparation method of a collagen and anti-propionibacterium acnes gamma protein combined preparation, and the preparation method of the collagen and anti-propionibacterium acnes gamma protein combined preparation comprises the following steps: preparing anti-propionibacterium acnes gamma protein; adding water into the anti-propionibacterium acnes gamma protein and the recombinant III-type collagen, stirring and mixing, adding glycerol and a neutralizing agent, and mixing to obtain a uniform solution; the preparation method comprises the following steps: dispersing carbomer with water, adding a preservative and a humectant, mixing, finally adding the uniform solution, and mixing to obtain the required collagen and anti-propionibacterium acnes gamma protein combined preparation. The combined preparation prepared by the method can be used for effectively treating acnes, is remarkable in curative effect, does not generate drug resistance, is short in treatment cycle, can quickly eliminate skin damage, promote cell growth and adhesion and repair skin barriers, and does not generate new rash after healing.

This invention belongs to the field of food processing technology, specifically relating to an oat bran β-glucan extract, its preparation method, and its application. The preparation method involves obtaining the oat bran β-glucan extract from oat bran through multi-stage enzymatic hydrolysis. The enzymes used in the multi-stage hydrolysis sequentially include pentosanase, thermostable α-amylase, and proteinase K. This invention, under ultrasound assistance, allows pentosanase to efficiently break down cell walls first, significantly increasing the release rate of β-glucan. Subsequently, thermostable α-amylase and proteinase K precisely remove starch and protein impurities sequentially, greatly improving the purity of the product. This process route is mild, highly specific, and operates continuously, reducing impurity residue while improving extraction efficiency, providing a reliable and cost-effective solution for the large-scale preparation of high-purity, highly bioactive oat β-glucan.

The application discloses coumarin compounds, and a preparation method and application thereof. The coumarin compound is selected from a compound shown in formula (A-A); wherein R1 is selected from a group shown in the formula, H or C1-C10 alkyl, wherein R 1′ oxalyl chloride ester selected from H or C1-C10, R 1” substituted or unsubstituted aryl; R2 is selected from a group shown in the formula or H, wherein R 2′ substituted or unsubstituted aryl; R3 is selected from a group shown in the formula, H or hydroxyl, wherein R 3′ C1-C10 carboxylic acid, R 3” substituted or unsubstituted aryl; and when R1 of the coumarin compound is selected from hydrogen, R3 cannot be selected from hydroxyl. The coumarin compound has simple structure, small molecular weight and good biological activity, can inhibit test bacteria in different degrees, and can be used as a potential new antibacterial agent.

The invention relates to a novel 1, 2, 4-oxadiazole compound (I) as a fungicide, in which A1, R1, R2, R3, R4 and R5 are defined in the specification. The invention also discloses a preparation method of the compound of the formula (I) and application of the compound as a fungicide. (I)

This invention belongs to the field of biomedicine, specifically relating to a method for the expression and purification of recombinant human tropoelastin. More specifically, it relates to a method for efficiently expressing human tropoelastin using *E. coli* as a host by constructing an expression plasmid containing a T7 promoter, and obtaining a high-purity product through a series of purification processes. The technical problem to be solved by this invention is to provide a human tropoelastin with intact structure and high biological activity that can be efficiently expressed in a prokaryotic expression system, as well as its preparation method and applications.

This invention provides a bovine FSH recombinant protein, a recombinant expression vector, and a method for preparing the same. The bovine FSH recombinant protein has the amino acid sequence shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, or SEQ ID NO.4. A method for preparing the bovine FSH recombinant protein involves expressing the coding gene shown in SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, or SEQ ID NO.8 in a host bacterium or host cell to obtain a low-glycemic FSH recombinant protein. This invention, through genetic engineering, obtains a bovine FSH recombinant protein by first constructing a vector and then artificially regulating post-translational N-glycosylation modification by introducing the Asn-X-Thr mutation of the N-glycosylation motif, achieving stable and efficient expression of low-glycemic FSH with significantly enhanced biological activity.

The present application relates to the technical field of biological medicine, in particular to an anti-tumor grifola frondosa water-soluble protein complex preparation and a preparation method thereof.The preparation raw materials of the complex preparation include the following components in parts by weight: grifola frondosa water-soluble protein 2-3 parts, fermentation preparation 0.8-1.2 parts, rosemary acid 0.1-0.2 parts.

This application discloses a liver peptide with liver-protective and eye-fatigue-relieving properties, its preparation method, and its applications, belonging to the field of biotechnology. The liver peptide contains at least one of DAIP, SPF, DIAP, SLP, AVGP, or NLP. The prepared liver peptide has multiple physiological functions: it can effectively activate ADH enzyme activity, reduce ALT and AST levels, and protect hepatocytes; it improves lipid metabolism by regulating the expression of SREBP-1c, FAS, and PPAR-α genes; it also has significant antioxidant capacity, reducing ROS levels and increasing SOD and CAT content; and it can upregulate RPE65 and LRAT gene expression, maintaining visual function. The method disclosed in this invention is simple and convenient to operate, and the obtained liver peptide can be widely used in functional products for liver protection and eye fatigue relief, providing an innovative solution for the high-value utilization of liver resources.

The invention relates to the technical field of biological medicine and gene therapy, in particular to a nucleic acid delivery system for targeting pancreatic beta cells and application of the nucleic acid delivery system. The composition comprises a nano-composite formed by a cationic polymer modified by a targeting ligand and siRNA (small interfering Ribonucleic Acid), the targeting ligand is a GLP-1 receptor targeting peptide; the cationic polymer is a PEI-PFA polymer; and the siRNA is the siRNA of the target DYRK1A (DYRK1A). The invention provides a brand-new pancreatic beta cell targeted delivery platform for gene therapy of type 2 diabetes mellitus. The platform realizes beta cell function regeneration by accurately regulating and controlling DYRK1A gene expression, has targeting property, safety and high efficiency, and provides a new strategy with wide clinical application prospect for diabetes treatment.

The present application relates to the technical field of mesoporous materials, and particularly to an interface pore rich-hydroxyl silica with monodispersity and uniform size, and a preparation and application thereof. The present application provides a preparation method of the interface pore rich-hydroxyl silica with monodispersity and uniform size. The preparation process is to perform a reaction in a water-oil two-phase system. The oil phase is an easily volatile non-water-soluble organic solvent such as n-hexane. Caprylic triglyceride is added to the oil phase. A non-surfactant pore-forming scheme is adopted. N-octanol and propyl silicate are used as auxiliary pore-forming agents. Sodium silicate is used as a silicon source. Ethyl acetate is used as a catalyst. The synthesis reaction is performed under the condition that the heating temperature is 25-45 DEG C. The prepared silica presents a spherical structure, has uniform particle size, high dispersity, controllable diameter, an open pore structure on the surface and rich silicon hydroxyl, and exhibits good biological safety. The interface pore rich-hydroxyl silica has a great application prospect in the fields of biomaterials, medical adjuvants, food, daily chemicals, cosmetics, electronics and the like.

This invention discloses an in-situ depolymerization modification composition of mineral-derived humic acid, its method, and its applications. The composition comprises a hydrogen bond acceptor and an acidic hydrogen bond donor, wherein the hydrogen bond acceptor is selected from one or more of choline compounds, betaine compounds, and quaternary ammonium salt compounds; and the acidic hydrogen bond donor is an aliphatic polycarboxylic acid or a hydroxycarboxylic acid. This method is simple, green, and efficient, yielding a product with low molecular weight, good water solubility, and strong resistance to hard water. It is suitable for various product forms such as solid fertilizers, liquid fertigation fertilizers, and soil conditioners, and has broad application prospects in modern agriculture and ecological restoration.

The application discloses an indole compound, a preparation method and application thereof, and the indole compound has the structure shown in formula (I). The indole compound has anti-inflammatory activity and bioactivity of promoting angiogenesis. Meanwhile, raw materials used in a synthesis method of preparing the indole compound by using a rhodium / copper bimetal catalyst are cheap and easy to obtain, and the preparation process is simple and easy to operate.

This invention discloses the application of a polysaccharide coating membrane in the preparation of cell culture products, belonging to the field of cell culture food technology. Specifically, after cells are seeded onto a scaffold, a polysaccharide coating membrane is formed around it. This can alleviate the phenomenon of cells detaching from the scaffold during culture, thereby ensuring the effective proliferation and differentiation of muscle stem cells and / or adipose stem cells on the scaffold, providing a new solution for the efficient and standardized production of cell culture products.

This invention provides a method for soil improvement in edible mushroom cultivation bases, relating to the field of edible mushroom technology. The method specifically includes the following steps: S1. Laying peat moss; S2. Spreading biochar; S3. Spreading fermentation liquid; S4. Laying rice straw or wheat straw; S5. Evenly spreading adjuvants; S6. Applying at multiple points. This invention improves soil structure and nutrient distribution through multi-layered organic matter and gradient mineral addition, enhancing aeration and water retention. Simultaneous use of ultraviolet disinfection and biological bacterial liquid effectively kills harmful pathogens while rapidly restoring beneficial microorganisms. Regular spraying of photosynthetic bacteria liquid and biological enzyme liquid maintains a healthy soil ecosystem. Multi-point application of compound bacterial agents activates the soil microenvironment. pH adjustment ensures the soil remains in optimal condition, increasing the yield and quality of edible mushrooms. Biological barriers and triple spraying effectively prevent pests and diseases, reducing the use of chemical pesticides and ensuring environmental safety.

The application discloses a kind of micropore pre-reaction and pesticide residue detection equipment of immunochromatography, belong to pesticide detection technical field, including shell and for raw material processing processing mechanism, shell is provided with for the pesticide residue of raw material detection detection mechanism and for the replacement of detected liquid replacement mechanism;The application is by integrating units such as broken jar, reference liquid jar, liquid pump and feeding pump in shell, constructs the automatic sample pretreatment system, when detecting, fruit and vegetable samples are broken in broken jar, equipment is pumped into special reference liquid by pH7.2-7.6 phosphate buffer solution, Tween-20, NaCl and gelatin, the reference liquid not only can efficiently extract nicotinamide pesticide in fruit and vegetable, more importantly, its mild component can effectively eliminate the interference of fruit and vegetable matrix to subsequent immune response, while protecting the biological activity of colloidal gold labeled antibody, lay foundation for subsequent high specificity binding, improve the accuracy and reliability of detection.

This invention discloses an aqueous solution of silk fibroin-hydrophobic active material aggregates and its preparation method, comprising the following steps: adding a hydrophobic active material with a planar conjugated structure to an aqueous dispersion system of high-crystalline silk fibroin aggregates; and inducing a conjugation reaction between the hydrophobic active material and the silk fibroin aggregates by stirring or homogenization to form silk fibroin-hydrophobic active material aggregates, thereby obtaining an aqueous solution of silk fibroin-hydrophobic active material aggregates. This invention utilizes the mutual conjugation reaction between high-crystalline silk fibroin at the nanoscale and a hydrophobic active material with a planar conjugated structure. Under high-speed stirring or homogenization, the two are induced to directly form nano-aggregates, and the hydrophobic active material is dissolved by utilizing the water dispersibility of silk fibroin. No other solvents, co-solvents, or carriers are required, and the method is simple and controllable.

The present application relates to a kind of carbonyl oxides azo compounds or its agriculturally acceptable salt and its preparation method and application, and its application as insecticide and fungicide, and part compound exhibits excellent fungicidal activity and nematocidal activity.

This invention belongs to the field of cosmetic raw material preparation technology, specifically relating to a method for decolorizing Xinjiang erythromycin, its products, and applications. The method includes: raw material pretreatment: pulverizing Xinjiang erythromycin and processing it using high-pressure instantaneous explosion technology; extraction: extracting the pretreated material with an ethanol-water solution, and obtaining a crude extract of Xinjiang erythromycin after solid-liquid separation; activated carbon decolorization: adding ethanol to the crude extract and mixing evenly, then adding activated carbon for decolorization treatment, followed by solid-liquid separation after decolorization; resin purification: loading the supernatant obtained after decolorization onto a resin column, performing gradient elution, collecting the ethanol eluent rich in phenolic acids, concentrating, and drying. This invention achieves an excellent balance between efficient decolorization and retention of highly active ingredients. It can strongly adsorb and remove dark pigments while significantly reducing the adsorption loss of the target phenolic acids.

This invention belongs to the fields of coordination chemistry, molecular magnetic materials, and biofunctional materials. Specifically, it discloses a three-dimensional iron-based metal-organic framework material, its preparation method, and its applications. The chemical formula of the three-dimensional iron-based metal-organic framework material is: [Formula omitted for brevity]. Wherein, is a divalent iron ion, M is a metal ion capable of forming dicyano coordination units, CN is a cyano-bridging ligand, L is a neutral nitrogen-containing bridging ligand, and G is a guest molecule. The material of this invention employs strong coordination bonds to construct a robust three-dimensional framework structure. The Fe(II) center is multiple-coordinated and fixed within the rigid framework by multidentate nitrogen-containing ligands and cyano-bridging anions. After multiple temperature-induced spin-state transition tests, the material of this invention maintains good reversibility in both its crystal structure and functional signal, without structural collapse, guest molecule loss, or ligand detachment. The material of this invention exhibits excellent cycling stability and reusability, making it more suitable for long-term operation in practical devices.

The present application belongs to the technical field of molecular biology, and relates to a truncated fish peptidoglycan recognition protein and application thereof. The amino acid sequence of the truncated fish peptidoglycan recognition protein is shown in Table SEQ ID NO. 1. The truncated fish peptidoglycan recognition protein provided by the present application has higher expression efficiency and higher yield in a prokaryotic expression system compared with a natural peptidoglycan recognition protein. The truncated fish peptidoglycan recognition protein has stronger binding capacity with peptidoglycan (PGN) and lipopolysaccharide (LPS), which are main components of cell walls of gram-positive bacteria and gram-negative bacteria. The bacteriostatic and agglutination capacity of the truncated fish peptidoglycan recognition protein on gram-positive bacteria and gram-negative bacteria is significantly improved. These characteristics show that the truncated fish peptidoglycan recognition protein has development potential as an aquatic immunopotentiator, and provides important support for subsequent application research.