1200results about How to "Improve biological activity" patented technology

The invention relates to a high-activity recombinant XVII type collagen as well as a rapid preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 1, and the nucleotide sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 2. The invention also provides a preparation method and application of the recombinant XVII type collagen. The high-activity recombinant XVII type collagen has good biological activity, has good effects of promoting cell adhesion, cell proliferation and cell migration, and can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines. The high-activity recombinant XVII type collagen gene is integrated to different positions of a yeast genome through three different vectors, and the copy number of a target gene is increased, so that the expression quantity of the recombinant XVII type collagen is remarkably increased, and the yield of the high-activity recombinant XVII type collagen is effectively increased.

The invention provides a recombinant humanized III-type collagen with a triple helix structure and preparation and application thereof, and belongs to the technical field of protein engineering, the amino acid sequence of the recombinant humanized III-type collagen comprises n core units, n is an integer of 1 or greater than 1, and the amino acid sequence of each core unit is as shown in SEQ ID NO: 1; the invention also provides a construction method of the expression system and a preparation method of the recombinant humanized III-type collagen. The invention also provides practical industrial application according to the characteristics of the protein. The recombinant humanized III-type collagen provided by the invention is high in purity, good in stability and high in biological activity, and experiments show that the recombinant humanized III-type collagen has various biological activities of promoting cell proliferation and migration and the like.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention relates to the technical field of cosmetic raw materials, in particular to a fibroin-active matter nano aggregate and a preparation method thereof.The preparation method of the fibroin-active matter nano aggregate is characterized by comprising the following steps that S1, a fibroin aggregate aqueous solution and large-molecular-weight active matter are obtained, mixing the fibroin aggregate aqueous solution and the high-molecular-weight active matter to obtain a first mixed solution; and S2, continuously stirring the first mixed solution for 2-240 minutes to obtain a target product, wherein the large-molecular-weight active matter comprises at least one of protein, polysaccharide, glycosaminoglycan, sugar alcohol and a compound of the protein, the polysaccharide, the glycosaminoglycan and the sugar alcohol, and the mass ratio of the silk fibroin aggregate in the silk fibroin aggregate water solution to the large-molecular-weight active matter is (100: 1)-(1: 2). The transdermal property of the active matter can be simply and effectively improved while the structure and the performance of the active matter are not changed.

The invention provides an anti-human CD117 monoclonal antibody and an application thereof. The anti-human CD117 monoclonal antibody comprises three heavy chain complementary determining regions CDR-H1, CDR-H2 and CDR-H3 and three light chain complementary determining regions CDR-L1, CDR-L2 and CDR-L3, the amino acid sequence of the CDR-H1 is as shown in SEQ ID NO: 1, the amino acid sequence of the CDR-H2 is as shown in SEQ ID NO: 2, the amino acid sequence of the CDR-H3 is as shown in SEQ ID NO: 3, the amino acid sequence of the CDR-L1 is as shown in SEQ ID NO: 4, the amino acid sequence of the CDR-L2 is as shown in SEQ ID NO: 5, and the amino acid sequence of the light chain complementary determining regions is as shown in SEQ ID NO: 6. The amino acid sequence of the CDR-L3 is as shown in SEQ ID NO: 6. Compared with the existing known anti-human CD117 monoclonal antibody, the antibody disclosed by the invention shows better cellular level biology, and is expected to show a good clinical effect in the aspect of preventing and treating related diseases.

The invention relates to the technical field of microorganisms, in particular to high-temperature-resistant bacillus aryabhattai and application thereof, the bacillus aryabhattai is named as LYGW-K2, and the preservation number of the bacillus aryabhattai is CCTCC (China Center for Type Culture Collection) NO: M2024963. Soil is collected in a field test field, lignocellulose degrading bacteria are obtained through enrichment culture, Congo red dyeing and aniline blue fading preliminary screening, then the degrading bacteria are sequenced by adopting 16srDNA through determination of the activity of cellulase, hemicellulase and ligninase of the degrading bacteria, a filter paper strip disintegration test and straw degradation rate research, and the content of the lignocellulose is determined. And finally, a strain of high-temperature-resistant bacillus aryabhattai LYGW-K2 with a relatively high degradation rate on lignin and cellulose is selected out. The bacillus aryabhattai is used for straw decomposition, can still keep excellent biological activity at a high composting temperature, generates cellulose degrading enzyme and lignin degrading enzyme, accelerates the straw decomposition process, and is good in high temperature resistance and obvious in straw degradation effect.

The invention provides recombinant IV-type collagen as well as a preparation method and application thereof, and relates to the technical field of biochemical engineering. The preparation method of the recombinant IV-type collagen comprises the following steps: obtaining a codon from amino acid of the recombinant IV-type collagen, further obtaining a target gene, and synthesizing the target gene onto an expression vector to obtain a plasmid; transferring the plasmid into an expression host, and screening a positive transformant to obtain an expression strain; and culturing the expression strain, and expressing the target protein to obtain the crude recombinant IV-type collagen. The high-purity recombinant IV-type collagen is prepared through a genetic engineering technology, and the recombinant IV-type collagen can significantly down-regulate inflammatory factors and reduce inflammation; heat injury can be reduced by up-regulating heat shock protein; after heat injury, the recombinant IV-type collagen can obviously lower metal matrix protease of heat injury cells, and also can obviously promote gene expression of type I collagen and type III collagen, improve skin elasticity and moisture content and delay skin aging.

The invention discloses burdock root fermentation liquor as well as a preparation method and application thereof in cosmetics, and belongs to the technical field of cosmetics. The preparation method provided by the invention comprises the following steps: inoculating a culture medium with pseudoalteromonas or schizosaccharomyces pombe for activation to obtain a seed solution; the seed solution is inoculated into a fermentation medium, burdock root powder is added, the adding concentration of the burdock root powder in the fermentation medium is 45-55 g / L, then fermentation is conducted to the end point, and after-treatment is conducted, the burdock root fermentation liquor is obtained. The burdock root fermentation liquor prepared by the preparation method disclosed by the invention has excellent anti-oxidation effect, anti-allergy soothing effect and tightening effect, also has certain healing promoting and repairing functions, is safe, non-toxic and small in irritation, and can be used for preparing anti-oxidation, anti-allergy and soothing cosmetic raw materials.

The invention discloses a production method of distiller's grain fermentation liquid fertilizer specially applied to roxburgh rose. The distiller's grain fermentation liquid fertilizer is prepared bythe step of evenly mixing 50 to 80 parts of distiller's grain fermentation extracting solution, 0.5 to 1 part of microelement, 5 to 10 parts of seaweed meal, 5 to 10 parts of natural active organism,2 to 5 parts of potassium bicarbonate, 2 to 5 parts of natural active organism and 2 to 5 parts of natural surface active agent. A preparation method of the distiller's grain fermentation extracting solution comprises the steps: evenly stirring 50 to 80 parts of distiller's grain, 50 to 150 parts of rice washing water, 1 to 2 parts of EM microflora fermentation liquid and 0.5 to 1 part of sugar, performing composting fermentation at the room temperature to obtain a distiller's grain fermentation material, stirring and extracting through a sodium carbonate water solution, filtering and performing evaporation and condensation on obtained filtrate until the density is 1.1 to 1.2 to obtain distiller's grain fermentation extracting solution. The distiller's grain fermentation liquid fertilizerdisclosed by the invention has a good use effect, meanwhile can effectively prevent pest and disease damage to the roxburgh rose and is high-efficiency environment-friendly liquid fertilizer.

The preparation method comprises the following steps: firstly, analyzing the amino acid sequence of an alpha 1 chain of the human XVII type collagen, and selecting partial amino acid fragments which are not easily degraded by endogenous or exogenous protease in non-transmembrane regions of different functional domains of the protein; the recombinant collagen gene fragment (Col17a) n is obtained by repeatedly splicing the recombinant collagen gene fragment (Col17a) n; then introducing homologous promoters from different methylotrophic yeasts to the 5'end of a recombinant collagen gene segment, optimally synthesizing selected (Col17a) n and promoter segments, obtaining a connecting segment by utilizing PCR cloning, connecting the collagen segment (Col17a) n, the promoter segments and a pPIC9K carrier skeleton segment by virtue of a Gibson Assessment seamless connection technology, and obtaining the recombinant collagen gene. And constructing to obtain the pichia pastoris expression plasmid pPIC9K-(Col17a) n of the recombinant human source XVII type collagen.

The invention discloses a recombinant VI-type collagen with antioxidant activity and application thereof, the recombinant VI-type collagen is a truncated protein of human VI-type collagen, and the amino acid sequence of the recombinant VI-type collagen is as shown in SEQ ID NO: 1. The recombinant type VI collagen can be expressed in an escherichia coli expression system, the expression yield is high, the tested biological activity (antioxidant activity, cell proliferation promoting activity and cell adhesion promoting activity) is obviously superior to that of natural type VI collagen, and the recombinant type VI collagen has excellent antioxidant activity, is an effective free radical scavenger, and can be used for preparing a biological preparation. The polypeptide can remove free radicals in the body, provides a molecular basis for some important physiological and pathological processes related to oxidation resistance, can be widely applied to the fields of food, health food, biological medicine and the like, and has good development and application prospects.

The invention discloses a high-strength silk fibroin / hydroxyapatite bone repair material and a preparation method thereof, and belongs to the field of biomedical materials. Methacrylic acid esterified silk fibroin and methacrylic acid esterified nano-hydroxyapatite are used as organic matter and inorganic filler, and the interface bonding capacity and dispersion stability between the organic component silk fibroin and the inorganic component hydroxyapatite are improved through photo-crosslinking; and performing high-temperature hot-pressing molding to obtain the silk fibroin / hydroxyapatite bone repair material. The bone repair material has relatively strong mechanical properties, osteogenesis induction capability, good biocompatibility and degradability, and has a wide application prospect in the field of bone defect repair.

The invention belongs to the technical field of biological medicines, and particularly relates to a pyruvate preparation for treating asthma and a preparation method of the pyruvate preparation. The pyruvate preparation is prepared from the following components in parts by weight: 10 to 12 parts of pyruvate, 10 to 12 parts of sodium chloride, 0.5 to 1 part of citric acid and 500 parts of water for injection. The pH value of the pyruvate preparation is 6 to 7. The pyruvate preparation is used for daily management of asthma patients, the purpose of inhibiting asthma is achieved by controlling increase of the number of inflammatory cells such as eosinophilic granulocytes and reducing inflammatory reaction, the effect of the pyruvate preparation is close to that of similar hormone medicine dexamethasone, and the effect of the pyruvate preparation is improved while the treatment purpose is achieved. The dependence and side effects of patients on hormone drugs are reduced.

The invention relates to the technical field of fertilizers, and discloses a fulvic acid fertilizer based on straw microbial fermentation and a preparation method and application thereof.The preparation method comprises the steps that straw is crushed and subjected to low-temperature plasma pretreatment, and pretreated straw is obtained; then, culturing the mixed microbial preparation to prepare a culture solution; then, the pretreated straw, a nutrient solution, a fermentation auxiliary agent and trichoderma are mixed for fermentation, and therefore a fermentation product is generated. The fermentation auxiliary agent is prepared from seaweed meal, bean flour, humus soil and molasses. And finally, carrying out solid-liquid separation on the fermentation product, and drying the separated solid part to finally obtain the fulvic acid fertilizer. Through combination of low-temperature plasma pretreatment and an innovative fermentation auxiliary agent, the preparation process of the fulvic acid fertilizer is optimized, the fertilizer efficiency of the fertilizer and the crop growth effect are improved, and meanwhile, the environment problem of agricultural waste is solved.

According to the method, a one-pot copolymerization method is adopted, MBAA and borax serve as cross-linking agents, zwitterionic polyelectrolyte hydrogel containing SBMA, PVA, THMA and AMPS is prepared, and the zwitterionic polyelectrolyte hydrogel has excellent adhesion performance and biocompatibility and is suitable for the field of biological medicine. According to the method, the physical characteristics of the hydrogel are enhanced due to crosslinking sites and hydrogen bonds of polyvinyl alcohol. The cationic and anionic groups of SBMA improve the adhesion performance, and the reaction monomer THMA provides hydrogen-bond interaction to further promote adhesion, so that the prepared hydrogel adhesive shows excellent adhesion performance and biocompatibility, and is suitable for biological medicine application of wound dressing, drug release systems, tissue engineering and the like.

The invention discloses a biological functional scaffold system driven by dual-mode printing, relates to the technical field of biomedicine, and is used for providing more accurate control on the aspects of porosity, cell attachment and surface modification. By integrating two advanced manufacturing technologies of 3D printing and aerosol spraying, the porosity and surface characteristics of the stent can be accurately controlled in the same printing process, and the cell attachment and proliferation environment is optimized. The cooperative working mechanism greatly improves the biocompatibility and mechanical strength of the biological scaffold, and further effectively promotes the tissue regeneration and repair efficiency. The whole manufacturing process is more efficient and accurate due to introduction of the control unit. The control unit automatically adjusts the printing speed and the spraying pressure based on real-time monitoring feedback, the uniformity and stability of the support are ensured, the error rate in the production process is reduced, and therefore the consistency and reliability of products are improved.

The invention discloses a microbial compound high-efficiency organic bacterial fertilizer which comprises the following raw materials: 10-20 parts of a microbial agent, 10-20 parts of humic acid, 3-10 parts of natural mineral powder, 1-7 parts of a plant extract, 2-4 parts of amino acid, 2-5 parts of a seaweed extract, 1-3 parts of chitosan, 3-7 parts of charcoal, 1-3 parts of chitin, 2-5 parts of potassium fulvate and 1-3 parts of lignosulfonate. The problems that existing organic bacterial manure is single in function, not remarkable in effect, high in preparation cost and the like are solved. The organic bacterial fertilizer has multiple functions of improving soil fertility, promoting crop growth, enhancing crop stress resistance and the like, and the preparation method is simple, easy to implement, low in cost and suitable for large-scale production.

The invention provides an application of a recombinant XVII type humanized collagen or a composition containing the recombinant XVII type humanized collagen in preparation of a preparation for preventing and / or treating lupus erythematosus, which can overcome the defect that more adverse reactions are generated in treatment in the existing scheme.

The invention relates to a preparation method of a placenta active factor as well as a product and application of the placenta active factor, and the preparation method comprises the following steps: soaking and rinsing a full-month normal delivery placenta, and homogenizing to obtain homogenate; repeatedly freezing and thawing the homogenate to collect a thaw solution, centrifuging the thaw solution, taking a supernatant, mixing the supernatant with a compound enzyme, and carrying out ultrasonic enzymolysis extraction to obtain an enzymolysis product; and centrifuging the enzymolysis product, taking the supernatant, and carrying out membrane filtration to obtain the placenta active factor. The compound enzyme comprises a combination of animal protein hydrolysis compound enzyme, alkaline protease, neutral protease and papain. The placenta tissue is extracted through cooperation of the specific compound enzyme and ultrasonic waves, the placenta tissue and the specific compound enzyme have a synergistic effect, the extraction efficiency can be further improved, the content of active ingredients in the extract is high, the biological activity is high, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention relates to daily chemical and pharmaceutical industries, in particular to a guanidyl-containing carboxymethyl chitosan quaternary ammonium salt derivative as well as a preparation method and application thereof. The chemical structural formula of the guanidyl-containing carboxymethyl chitosan quaternary ammonium salt derivative is shown in the specification. The prepared guanidyl carboxymethyl chitosan-containing quaternary ammonium salt derivative has good antioxidant activity and can be applied to preparation of an antioxidant, the preparation method is simple, convenient and efficient, the biological activity of chitosan is enhanced, the application range of chitosan is widened, and the guanidyl carboxymethyl chitosan-containing quaternary ammonium salt derivative can be widely applied to the fields of cosmetics, functional food and medicine. # imgabs0 #

The invention relates to the technical field of cell activity detection, in particular to an umbilical cord blood stem cell activity detection method and system.The method comprises the following steps that cells are separated from a fresh umbilical cord blood sample in a sterile environment, cell activity preliminary identification is conducted on the separated stem cells, and a cell activity evaluation result is generated; screening cells with activity meeting a preset standard, and performing function evaluation to obtain a screened cell set. According to the method, through accurate preliminary cell viability identification and function evaluation, the scheme optimizes the selection standard of umbilical cord blood stem cells, effectively screens out cells with high regeneration capacity and biological activity, enhances the success rate of cell therapy and reduces the risk caused by improper selection, and by analyzing the gene regulatory network of the screened cells, the cell therapy effect is improved. The functionality is accurately evaluated, it is ensured that only the most potential cells are used for subsequent culture and clinical application, the curative effect and the product quality are remarkably improved, and the culture efficiency and the success rate are further improved through an optimized culture scheme and a monitoring system.

The invention discloses a preparation method of sturgeon roe peptide freeze-dried powder with an anti-aging effect. The preparation method specifically comprises the following steps: (1) unfreezing sturgeon roes, adding water, uniformly stirring, grinding and crushing, and adjusting the pH value; (2) heating and naturally cooling; (3) adding alkaline protease, uniformly stirring, carrying out enzymolysis, and adding starch sodium octenylsuccinate and vitamin E powder; (4) carrying out enzyme inactivation, naturally cooling, and adjusting the pH value; (5) carrying out rough filtration and centrifugal treatment, taking supernate, adsorbing, filtering and taking filtrate; and (6) carrying out plate frame filtration, taking filtrate, carrying out vacuum freeze drying, screening and packaging, thereby obtaining the product. The preparation method disclosed by the invention has the advantages of simplicity and convenience in operation, low cost, high embedding yield and the like, and has important significance in improving the stability and biological activity of the sturgeon roe peptide. Moreover, the sturgeon roe peptide freeze-dried powder has an anti-aging effect, and can be widely applied to oral liquid, solid beverages, tablet candies and the like in common foods.

The invention discloses a preparation method and application of a composite nitrogen, phosphorus and sulfur removal autotrophic filter material, and relates to the technical field of sulfur autotrophic filter materials, and the preparation method comprises the following steps: stacking and combining a basic filter material, a catalyst loaded filter material, a strain-heterojunction loaded filter material and a magnesium oxide microsphere filter material from top to bottom, the composite nitrogen, phosphorus and sulfur removal autotrophic filter material is obtained; in the stacking combination process of the composite nitrogen, phosphorus and sulfur removal autotrophic filter material, the stacking thickness ratio of the basic filter material to the catalyst loaded filter material to the strain-heterojunction loaded filter material to the magnesium oxide microsphere filter material is 10: (3-5): (2-4): (4-5). The efficient and low-cost composite nitrogen, phosphorus and sulfur removal autotrophic filter material is developed according to the synergistic treatment requirements of nitrogen and phosphorus pollution and PNP pollution.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to an anti-saccharification and anti-aging aronia melanocarpa fruit composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 150 to 400 parts of aronia melanocarpa fruit fermentation liquor, 10 to 80 parts of lycium ruthenicum, 10 to 80 parts of ginseng, 0.5 to 2.5 parts of grape seed extract, 10 to 70 parts of collagen peptide, 0.5 to 4 parts of sodium hyaluronate, 0.1 to 0.8 part of resveratrol and 0.03 to 0.3 part of yeast extract. The content of type I collagen peptide in the skin is increased, the generation of in-vitro anti-glycation end products is inhibited, and the anti-glycation and anti-aging effects are achieved.

The invention discloses a class of radiopharmaceutical labeled precursors targeting prostate specific membrane antigens, the structure of the radiopharmaceutical labeled precursors is shown as the following: # imgabs0 #, a precursor reagent RESCA-PSMA-1802 targeting prostate specific membrane antigens is synthesized for the first time, the reagent can be labeled by using an 18F-Al method to prepare a positron imaging agent 18FAl-RESCA-PSMA-1802, and the reagent can be used for preparing the prostate specific membrane antigens. The polymer has good stability and biological activity in vivo and in vitro, has good targeting property on prostate cancer cells, can reflect tumor recurrence or metastasis conditions, and is very suitable for PET imaging.

The invention relates to the technical field of medical instruments, and discloses an application of a collagen-gelatin combined VEGF (vascular endothelial growth factor) mimic peptide in promoting growth of blood vessels, the collagen-gelatin combined VEGF mimic peptide is QKCMP-Enhancer, and the sequence of the collagen-gelatin combined VEGF mimic peptide is Ac-KLTWQELYQLKYKGI-(GPO) 5-(KR) 4-(RGD) 4-(KR) 4-GGG-(Gly) 3-NH2. The invention discloses a collagen-gelatin combined VEGF (vascular endothelial growth factor) mimic peptide which shows remarkable biological activity in the aspects of promoting angiogenesis, stimulating cell proliferation and inhibiting cell apoptosis. A new way and a new strategy are developed for treating related diseases; the collagen-gelatin is combined with the VEGF mimic peptide, so that a safer, more efficient and controllable angiogenesis method can be realized, and the collagen-gelatin combined VEGF mimic peptide has remarkable clinical application potential.

The invention discloses an osthole compound, a metal complex and a preparation method and application of the osthole compound, the osthole compound (OST-Alkyne) has remarkable anti-tumor biological activity, osthole (OST) and a cyclometalated iridium precursor are further connected through a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, higher anti-tumor activity is shown, and the osthole compound has a good application prospect. Mitochondria is targeted, and tumor is killed by reducing mitochondrial membrane potential and inducing cell autophagy and necrotic apoptosis; according to the invention, natural products are combined with metals, so that not only are good selectivity and anti-proliferation ability to brain glioma cells realized, but also toxic and side effects to normal cells are overcome, a new design idea is provided for novel metal drugs to treat brain glioma, and the application prospect is wide.

The invention discloses a construction method and application of a ratio-dependent electrochemical luminescence (ECL) biosensor based on an antifouling red cell membrane, and belongs to the technical fields of photoelectric analysis, biosensing and interface antifouling. In the invention, the erythrocyte membrane with super-hydrophilicity and low membrane protein content is used as an efficient antifouling interface, and the good biocompatibility of the erythrocyte membrane avoids the influence of a chemical antifouling coating on the biological activity of the ECL sensor. Meanwhile, the electropolymerized polyaniline nanowire is embedded into the antifouling erythrocyte membrane, so that the chemical stability and the conductivity of the antifouling erythrocyte membrane are enhanced, and the application of the antifouling erythrocyte membrane in ECL analysis is expanded. C3N4 is introduced as an interior label for eliminating background signals and external interference, so that the detection precision of the biosensor is improved. On the basis, the constructed ECL antifouling biosensor realizes ultra-sensitive detection of human breast cancer cells MCF-7 in human serum, and the detection limit is as low as 3 cells / mL.

The invention provides a preparation method of a mineralized collagen scaffold material with a bionic structure, and belongs to the field of biomedical engineering.The preparation method comprises the steps that an amorphous calcium phosphate solution stabilized by polyacrylic acid is prepared, and the molar ratio of Ca to P in the amorphous calcium phosphate solution is 1.67: 1; preparing a collagen solution containing noradrenaline; carrying out isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense membrane, and stretching the dense membrane to generate 120-200% deformation, so as to obtain a collagen membrane with a bionic structure; and soaking the collagen membrane in the amorphous calcium phosphate solution, and then performing freeze drying to obtain the noradrenaline-mineralized collagen scaffold material. The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenesis inductivity and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering. The invention further provides the mineralized collagen scaffold material with the bionic structure and application of the mineralized collagen scaffold material.

The invention relates to the technical field of functional composite materials, and discloses a preparation method of polylactic acid porous microspheres and application of the polylactic acid porous microspheres in stem cell inoculation, and the preparation method comprises the following steps: (1) dissolving chitosan in an acetic acid aqueous solution, adding bioactive glass nanoparticles, and performing ultrasonic treatment to obtain an aqueous phase solution; (2) dissolving PLGA or PLA in dichloromethane, and adding an emulsifier to obtain an oil phase solution; (3) dropwise adding the water-phase solution into the oil-phase solution to obtain a water-in-oil primary emulsion; (4) dropwise adding the water-in-oil primary emulsion into a PVA aqueous solution to obtain a double emulsion; and (5) stirring the double emulsions, carrying out centrifugal separation, drying, and immersing in a glutaraldehyde solution for cross-linking treatment to obtain the porous microspheres. The porous microspheres are prepared by a double-emulsion solvent evaporation method, and have more uniform pore size distribution and higher biological activity, so that the porous microspheres can be used as an excellent stem cell inoculation carrier to meet the attachment and proliferation of high-proportion cells.