18 results about "Alkaline phosphatase" patented technology

A method for improving saline-alkaline soil using Salvia miltiorrhiza combined with biochar, comprising the following steps: S1. Soil preparation: applying harmless treatment organic fertilizer as a base fertilizer, evenly applying biochar according to a proportion, plowing and tilling the soil, and, according to planting density and topographical conditions, planning ridge spacing and ridge width of double high ridges; S2. Salvia miltiorrhiza planting: performing Salvia miltiorrhiza seedling transplantation using a seedling transplantation method and hole planting means, digging holes on ridge surfaces, trimming overly long parts of seedling roots, planting seedlings vertically, and covering with soil; S3. field management after transplanting: performing fertilization, weeding, and top dressing management. The present method for improving saline-alkaline soil using Salvia miltiorrhiza cultivation combined with biochar application reduces soil pH and total salt content, increases soil sucrose enzyme activity, increases soil alkaline phosphatase activity, and alters soil microbiome structure.

The invention relates to the technical field of kits, in particular to a magnetic particle chemiluminiscence detection kit for insulin and a preparation method of the magnetic particle chemiluminiscence detection kit for insulin, specifically, a reagent M, a reagent R2 and a reagent R1 are used for forming the kit, and the reagent M is a magnetic particle working solution containing streptavidin magnetic beads; the reagent R2 is an enzyme-labeled working solution containing an insulin antibody labeled by alkaline phosphatase; the reagent R1 is a working solution containing a biotin-labeled insulin monoclonal antibody. Through verification, according to the chemiluminescence kit for insulin provided by the invention, the test sensitivity reaches 2.00 mu IU / mL, the test repeatability for high and low value samples is less than 3%, and the linear correlation with Rogowski reaches 0.98 within the concentration range of 2.00-450 mu IU / mL. The invention also provides a preparation method of the insulin chemiluminescence kit, the production cycle is greatly shortened, the batch difference is easy to control, and the production work of enterprises is facilitated.

The application provides a skin interstitial fluid miRNA multicolor visualization semi-quantitative detection method, which comprises the following steps: incubating a skin interstitial fluid sample with magnetic nano probes of surface immobilized hairpin probes H1, opening H1 and triggering a subsequent hybridization chain reaction of hairpin probes H2 and H3 in the presence of target miRNA, and constructing a biotin-rich DNA complex on the surface of the magnetic beads; performing enzyme labeling by using an alkaline phosphatase-streptavidin coupling compound, and catalyzing ascorbic acid phosphate to generate ascorbic acid potassium iodate, so that the color of the solution changes, and high-sensitivity detection of the target miRNA is realized. The multicolor visualization detection method can produce near-full-spectrum color changes by combining the gold nano double-cone local surface plasmon resonance property change, and is beneficial to improving the portability of the sensor and the accuracy of the visual analysis.

The invention relates to a meadow alkaline halophilic bacterium LH-SA-1 capable of absorbing sodium and reducing salt and alkali as well as a saline-alkali soil remediation microbial inoculum and application of the meadow alkaline halophilic bacterium LH-SA-1. The LH-SA-1 is separated from severe saline alkali soil in Jilin Baicheng, the classification name of the LH-SA-1 is Alkalihalophilus lindianensis, and the preservation number of the LH-SA-1 is CGMCC (China General Microbiological Culture Collection Center) No. 32707. The LH-SA-1 can tolerate 3%-45% of various salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate and a strong alkali environment with the pH value of 9-12. The granular saline-alkali soil remediation microbial inoculum is prepared through composite sodium ion induced fermentation and double-membrane coupling concentration technologies in combination with a multifunctional carrier, the bacterial quantity is larger than or equal to 200 * 10 < 8 > CFU / g, and the catalase activity is larger than or equal to 130000 U / g. The microbial agent is applied to soda saline-alkali soil or coastal composite saline-alkali soil in northeast China, has multiple functions of remarkably reducing the saline-alkali content of the soil, improving the porosity of the soil, improving the activity of enzymes such as catalase, peroxidase, urease and alkaline phosphatase in the soil, improving the content of indoleacetic acid in the soil and the like, and provides an efficient and low-cost biological solution for saline-alkali remediation.

The application relates to a hepcidin chemiluminescence immunoassay kit and a non-diagnostic method for detecting hepcidin, the kit comprising a calibrator, a quality control, an integrated bifunctional antibody reagent, a magnetic particle reagent, a cleaning solution and a chemiluminescence substrate solution; the integrated bifunctional antibody reagent is a buffer containing HEP-specific antibodies covalently connected with fluorescein isothiocyanate and alkaline phosphatase through pH-sensitive connecting bonds; the magnetic particle reagent is a magnetic microsphere suspension coated with a goat anti-fluorescein isothiocyanate antibody; the cleaning solution is a buffer containing a surfactant composition, and the surfactant composition comprises nonionic surfactants, zwitterionic surfactants and high-molecular nonionic surfactants. The hepcidin chemiluminescence immunoassay kit has the effects of ultra-low background noise, ultra-high sensitivity, ultra-wide linear range and simplified operation of end users by means of the integrated bifunctional antibody reagent and the surfactant composition cleaning solution.

The application belongs to the technical field of biological detection, and particularly relates to a detection system of foodborne pathogenic bacteria and application thereof. The detection system of foodborne pathogenic bacteria comprises a composite response material, a hyaluronic acid hydrogel containing L-ascorbic acid-2-phosphoric acid trisodium salt and a bacteriophage; the composite response material comprises ZIF-90 nanoparticles and hyaluronidase and alkaline phosphatase encapsulated in the ZIF-90 nanoparticles. The application constructs a dual-mode paper-based visual detection system based on the combination of specific recognition of a bacteriophage and ZIF-90 response release mechanism, and realizes high-specificity detection of target pathogenic bacteria. Compared with the defects that nucleic acid amplification or immunological methods are difficult to distinguish live bacteria from dead bacteria, the application only produces a signal response to live bacteria that can be infected by a bacteriophage, and significantly improves the authenticity and reliability of detection results.

The invention relates to the technical field of microbial fertilizers, in particular to a compound microbial fertilizer for deacidification and recarburization of red and yellow soil and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer is prepared from the following raw materials in parts by mass: 40 to 60 parts of livestock manure, 30 to 50 parts of soybean meal, 1 to 3 parts of lime powder, 1 to 3 parts of potassium mineral powder, 1 to 2 parts of bentonite, 1 to 3 parts of compound trace element agent, 10 to 30 parts of bamboo charcoal powder, 5 to 15 parts of corn starch, 5 to 15 parts of sodium pyrophosphate, 1 to 2 parts of alkaline phosphatase, 1 to 3 parts of dopamine and 1 to 5 parts of microbial agent. The obtained compound microbial fertilizer not only can effectively adjust the acid environment of red and yellow soil and continuously increase the carbon reserve of the soil, but also can provide comprehensive guarantee for plant growth by improving the physical structure and biological activity of the soil, and finally achieves the target of increasing the yield and improving the quality of crops; and the preparation method has the advantages of easily available raw materials, feasible process and good application prospect.

The application belongs to the technical field of biomedicine, and specifically discloses a squamous cell carcinoma antigen determination kit and application, which comprises anti-reagent A, anti-reagent B and magnetic micro-particle reagent, wherein the anti-reagent A is a biotin-labeled squamous cell carcinoma antigen antibody; the anti-reagent B is an alkaline phosphatase-labeled squamous cell carcinoma antigen antibody; the squamous cell carcinoma antigen determination kit can effectively detect the concentration of squamous cell carcinoma antigen (SCC) in serum and plasma samples, and is mainly used for the treatment monitoring of cervical cancer, non-small cell carcinoma and the like, and has a wide application prospect.

The invention relates to an immortalized cell line of human dermal papilla cells as well as a construction method and application of the immortalized cell line. The human dermal papilla cells are named as DYZ-20, the immortalized cell line is preserved in the China Center for Type Culture Collection on July 2, 2025, survival is detected, and the preservation number is CCTCC NO: C2025204. The immortalized cell line of the human dermal papilla cells can normally express alkaline phosphatase and a dermal papilla characteristic gene, and hair can grow after the immortalized cell line is transplanted into mouse skin.

The invention provides an external traditional Chinese medicine composition with a bacteriostatic effect and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition comprises 20-40 parts of pepper, 20-40 parts of fructus cnidii, 10-30 parts of ligusticum, 5-15 parts of pogostemon cablin, 20-40 parts of schizonepeta, 10-30 parts of senecio scandens and 20-40 parts of mint. A response surface experiment design is adopted to optimize an extraction process of traditional Chinese medicinal materials and medicine residues, and the content of effective components such as total flavonoids in the extract is increased; the oil-soluble matrix and the water-soluble matrix are selected, so that the water-soluble medicine components and the oil-soluble medicine components can be better mixed, and the stability is high. The traditional Chinese medicine composition disclosed by the invention can inhibit the formation of bacterial cell membranes and destroy the structures of the bacterial cell membranes, and the permeability of the cell membranes is improved, so that macromolecular substances and proteins are leaked; the intracellular ATP content is reduced, and the extracellular alkaline phosphatase is increased, so that the growth of bacteria is finally inhibited or the bacteria are killed. According to the extraction process, effective components of the medicine are extracted to the maximum extent, and efficient utilization of the medicinal materials is guaranteed.

The invention provides a GDF-15 (growth differentiation factor-15) magnetic particle chemiluminescence immunoassay quantitative detection kit, which belongs to the field of in vitro diagnosis, and comprises a magnetic particle suspension, a biotin-labeled GDF-15 capture antibody solution, an alkaline phosphatase-labeled GDF-15 detection antibody solution, a calibrator, a chemiluminescence solution and the like. The kit provided by the invention has the advantages of rapidness and simplicity in operation, high sensitivity and repeatability, large measurement range and good stability, and can make up for the defects of the existing detection method.

This invention discloses a method for detecting organophosphorus pesticides using a self-cascaded Pt@Fe-CDs nanozyme, belonging to the field of chemical analysis and detection technology. This method synthesizes Pt@Fe-CDs nanozymes using iron-doped carbon dots (Fe-CDs) as a reducing agent and template. Pt@Fe-CDs simultaneously possess ascorbic acid oxidase-like and peroxidase-like activities. Under the catalysis of alkaline phosphatase, they hydrolyze L-ascorbic acid-2-phosphate trisodium salt to AA. Based on the fact that organophosphorus pesticides can inhibit ALP activity and hinder AA2P dephosphorylation, leading to a reduction in AA and thus causing changes in fluorescence and colorimetric signals, a simple and sensitive dual-signal fluorescence / colorimetric sensing platform for organophosphorus pesticides is constructed, with a detection limit of 0.28-0.53 ng / mL. The colorimetric and fluorescence biosensors established in this invention exhibit excellent performance in terms of sensitivity, specificity, and accuracy in actual samples.

The invention discloses a kit for detecting monocyte chemotactic protein-1 by a chemiluminescence method. The kit comprises a magnetic bead working solution, an enzyme working solution, an analysis buffer solution and a calibrator, the magnetic bead working solution comprises a mouse anti-human monocyte chemotactic protein-1 specific monoclonal antibody coated with streptavidin magnetic beads, an MCP-1 antibody and a magnetic bead buffer solution, and the concentration of the MCP-1 antibody in the magnetic bead working solution is 0.5 mu g / mL-5 mu g / mL; the enzyme working solution comprises an alkaline phosphatase labeled mouse anti-human monocyte chemotactic protein-1 specific monoclonal antibody and an enzyme buffer solution; the analysis buffer solution contains an animal serum component, and the calibrator contains a monocyte chemotactic protein-1 component. According to the chemiluminiscence detection kit for the monocyte chemotactic protein-1, the MCP-1 antibody is added into the magnetic bead working solution, so that the linear proportion of a working calibration curve is better, and the repeatability of a high-concentration sample is obviously improved.

This invention relates to the field of probiotic development and utilization technology, specifically to a probiotic strain of *Lactobacillus plantarum* SWUN3927 and its applications. The *Lactobacillus plantarum* (… Lactobacillus plants SWUN3927, isolated from colostrum of Tibetan yaks, was used in this invention to identify the probiotic effects of *Lactobacillus plantarum* SWUN3927 through animal experiments. The results showed that *Lactobacillus plantarum* SWUN3927 is resistant to acid and bile salts and has an inhibitory effect on pathogenic bacteria; its hydrophobicity is 74.67% and its self-aggregation is 58.98%, demonstrating that *Lactobacillus plantarum* SWUN3927 of this invention has good intestinal adhesion and possesses the potential of a probiotic. This invention verifies that *Lactobacillus plantarum* SWUN3927 can effectively regulate animal immunity, improve the body's antioxidant capacity, increase lysozyme content and alkaline phosphatase activity, improve daily weight gain, and ultimately improve animal growth performance.

The invention relates to the technical field of microbial complex microbial agents, in particular to peony nanocarbon liquid EM flora microbial agent composite compost which comprises the following raw materials in parts by weight: 2-4 parts of streptomyces microflavus zymophyte liquid, 2-4 parts of bacillus firmus zymophyte liquid, 1-3 parts of rhodovulum sulfidophilum culture liquid and 1-3 parts of nanocarbon solution. The peony fertilizer is prepared from 3-5 parts of kitchen waste filter residues, 10-12 parts of animal feather meal, 150-250 parts of decomposed cow dung, 80-120 parts of peanut straw, 2-4 parts of oil residues, 20-40 parts of feather meal, 10-12 parts of slaked lime, 2-4 parts of nitrogen, 20-40 parts of soybean meal, 20-40 parts of soybean curb residues, 4-6 parts of boric acid, 20-30 parts of phosphorus, 2-4 parts of magnesium sulfate, 2-4 parts of zinc oxide and 2-4 parts of potassium permanganate. Meanwhile, the granular structure of the soil is improved, the pH value of the soil is reduced, the EC value is increased, the activities of urease, sucrase, neutral phosphatase, alkaline phosphatase and catalase in the soil are improved, the photosynthesis of the peony is promoted, and the economic benefits of peony planting are increased.

The invention discloses a broad-spectrum Chinese herbal medicine compound for resisting main pathogenic bacteria of prawns as well as a preparation method and application of the broad-spectrum Chinese herbal medicine compound, and belongs to the technical field of aquaculture disease control. The compound consists of sanguisorba officinalis, myrobalan, coptis chinensis and smoked plum in a mass ratio of 1: 4: 4, and is prepared by the steps of crushing and sieving the raw materials, mixing in proportion, decocting in water, concentrating and sterilizing. The compound has a remarkable broad-spectrum antibacterial effect on six main pathogenic bacteria causing common diseases such as penaeus vannamei royal death disease, red-leg disease, luminescence disease and enteritis disease, the average inhibition zone reaches 22.08 mm, the minimum inhibitory concentration is as low as 2.67 mg / mL, the safety is high, the median lethal dose is larger than 10000 mg / kg, and the actual dosage is far lower than a toxicity threshold value. Meanwhile, the compound also can obviously improve the activity of acid phosphatase and alkaline phosphatase of the prawns to two times that of a control group, and the immune protection rate reaches 46.67%. When the feed additive is added into a prawn feed according to the addition amount of 1300-1500mg / kg, common bacterial diseases of prawns can be effectively prevented and treated, and hepatopancreas and midgut tissues of the prawns are not damaged.

The invention belongs to the technical field of biomedical materials and oral implantation, and particularly relates to a titanium alloy oral implant with a gradient porous structure and a partitioned modified surface and a preparation method of the titanium alloy oral implant. The implant is totally divided into an upper area and a lower area, the lower area adopts an innovative three-stage gradient porous structure, the neck edge area is provided with small holes, the middle transition area is provided with middle holes, and the root area is provided with large holes; after the implant is embedded into a jaw bone part, the elasticity modulus is gradually reduced from the crown part to the root part, the implant is matched with the mechanical property of a natural alveolar bone, and the stress shielding effect is effectively reduced. The surface of the implant is subjected to partitioned modification treatment through a femtosecond laser technology, and adhesion and proliferation of mouse fibroblasts are remarkably promoted after the upper region is treated; a specific micro-nano composite structure is formed after the lower region is treated, so that the proliferation rate of osteoblasts and the activity of alkaline phosphatase can be remarkably improved. Through the structure-function integrated design, mechanical suitability optimization, soft tissue closure enhancement and bone combination efficiency improvement are achieved at the same time.