72 results about "Alkaline phosphatase" patented technology

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention discloses a preparation method and application of a framework nucleic acid synergistic enhanced click chemistry and DNAzyme catalytic electrode. According to the present invention, the azide group modified split G-quadruplex fragment A is assembled on the double-stranded DNA (dsDNA) edge of the DNA tetrahedron through the Hoogsteen hydrogen bond, such that the reasonable spatial distribution of the reactants is achieved, and the click chemical reaction efficiency on the interface is improved; under the catalytic condition of Cu (I), the alkyne group modified split G-quadruplex fragment B and the fragment A assembled on the tetrahedral scaffold form a complete G-quadruplex through a click chemical reaction. And further, the compound is compounded with Hemin, so that the formed G-quadruplex / hemin compound has peroxidase-like activity. Meanwhile, the nucleic acid framework with the strong electronegativity characteristic further enhances the response current of catalytic oxidation TMB, and sensitive detection of Cu < 2 + > and alkaline phosphatase is achieved.

The invention discloses a nano-material-based nitrogen-phosphorus-potassium compound fertilizer as well as a preparation method and application thereof, and belongs to the field of plant nutrition and soil science research. According to the invention, the nano biochar prepared through nano grinding after pyrolysis of the rice straw is directly mixed with three chemical components, namely the urea, the ammonium dihydrogen phosphate and the potassium chloride, and the sodium bentonite, and the key points of the effects are triple effects of promoting early growth and rapid ripening, enhancing tillering and advancing heading. After the nano biochar is matched with balanced NPK nutrition (such as NPK-II), the activity level of soil key enzymes, including catalase (CAT), alkaline phosphatase (AKP), urease (UE), sucrase (SC) and the like, is remarkably improved. Besides, the composite particles are constructed through the cooperation of the nano-charcoal and the bentonite, and a low-temperature drying process is adopted, so that moisture absorption and caking can be inhibited, and the storage stability and field application performance of the fertilizer are remarkably improved.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The present invention relates to the technical field of bone tissue engineering scaffolds, and discloses a modified polycaprolactone scaffold and a preparation method thereof. The modified polycaprolactone scaffold comprises the following preparation raw materials: polycaprolactone, bioactive ceramics, solvent I and composite hydrogel; the composite hydrogel comprises a hydrogel solution and ZIF‑8 surface-modified polyester microspheres dispersed therein. The modified polycaprolactone scaffold provided by the present invention has a compressive strength higher than 20MPa, low in vitro cytotoxicity, high biosafety, no risk of hemolysis, can well induce cells to secrete alkaline phosphatase, has osteogenic activity, can also exert a long-term antibacterial effect, and is suitable for the repair and regeneration of bone tissue defects under infection.

A hardenable (e.g. dental) compositions is described comprising an encapsulated material. The encapsulated material comprises a basic core material and an inorganic shell material having certain viscosity criteria. Also described are dispensing devices and kits are described comprising a hardenable (e.g. dental) composition comprising a liquid material and an encapsulated material wherein the encapsulated material comprises a basic core material and an inorganic shell material comprising a metal oxide surrounding the core. The dispensing devices and kits can facilitate the methods of applying the hardenable composition. The hardenable or hardened (e.g. cured) composition can provide various technical effects such as a delayed release of a basic core material, a delayed increase in basicity, promoting remineralization of a tooth or bone structure, and increasing the average alkaline phosphatase (ALP) activity of pulp cells. In some embodiments, the composition is a dental (e.g. sealant) composition for application to a tooth structure.

A method for improving saline-alkaline soil using Salvia miltiorrhiza combined with biochar, comprising the following steps: S1. Soil preparation: applying harmless treatment organic fertilizer as a base fertilizer, evenly applying biochar according to a proportion, plowing and tilling the soil, and, according to planting density and topographical conditions, planning ridge spacing and ridge width of double high ridges; S2. Salvia miltiorrhiza planting: performing Salvia miltiorrhiza seedling transplantation using a seedling transplantation method and hole planting means, digging holes on ridge surfaces, trimming overly long parts of seedling roots, planting seedlings vertically, and covering with soil; S3. field management after transplanting: performing fertilization, weeding, and top dressing management. The present method for improving saline-alkaline soil using Salvia miltiorrhiza cultivation combined with biochar application reduces soil pH and total salt content, increases soil sucrose enzyme activity, increases soil alkaline phosphatase activity, and alters soil microbiome structure.

The invention relates to the technical field of kits, in particular to a magnetic particle chemiluminiscence detection kit for insulin and a preparation method of the magnetic particle chemiluminiscence detection kit for insulin, specifically, a reagent M, a reagent R2 and a reagent R1 are used for forming the kit, and the reagent M is a magnetic particle working solution containing streptavidin magnetic beads; the reagent R2 is an enzyme-labeled working solution containing an insulin antibody labeled by alkaline phosphatase; the reagent R1 is a working solution containing a biotin-labeled insulin monoclonal antibody. Through verification, according to the chemiluminescence kit for insulin provided by the invention, the test sensitivity reaches 2.00 mu IU / mL, the test repeatability for high and low value samples is less than 3%, and the linear correlation with Rogowski reaches 0.98 within the concentration range of 2.00-450 mu IU / mL. The invention also provides a preparation method of the insulin chemiluminescence kit, the production cycle is greatly shortened, the batch difference is easy to control, and the production work of enterprises is facilitated.

The application provides a skin interstitial fluid miRNA multicolor visualization semi-quantitative detection method, which comprises the following steps: incubating a skin interstitial fluid sample with magnetic nano probes of surface immobilized hairpin probes H1, opening H1 and triggering a subsequent hybridization chain reaction of hairpin probes H2 and H3 in the presence of target miRNA, and constructing a biotin-rich DNA complex on the surface of the magnetic beads; performing enzyme labeling by using an alkaline phosphatase-streptavidin coupling compound, and catalyzing ascorbic acid phosphate to generate ascorbic acid potassium iodate, so that the color of the solution changes, and high-sensitivity detection of the target miRNA is realized. The multicolor visualization detection method can produce near-full-spectrum color changes by combining the gold nano double-cone local surface plasmon resonance property change, and is beneficial to improving the portability of the sensor and the accuracy of the visual analysis.

The present invention discloses stress-resistant Geodermatophilus resistens and its application. The present invention provides stress-resistant Geodermatophilus resistens BI304, which has a registration number of CGMCC No. 25921 in the General Microbiological Center of the China Microbiological Culture Collection Administration. The strain BI304 of the present invention has alkaline phosphatase activity and is a functional new species of Geodermatophilus resistens, which has broad application prospects in garbage disposal, soil remediation, desert control, soil quality improvement, etc.

The invention belongs to the field of nano-materials, and particularly relates to a nano-enzyme with a spinel structure as well as application and a detection method of the nano-enzyme. The spinel-structured nano enzyme has oxidase-like activity or peroxidase-like activity, and is used for preparing oxidase-like, peroxidase-like, reagents, kits, materials, sensing platforms or sensors for detecting organic matters or inorganic matters. The method for detecting the organic matter or the inorganic matter based on the spinel structure nano enzyme has the advantages of high sensitivity, rapidness, low cost and strong specificity, and is particularly suitable for constructing a detection platform by taking alkaline phosphatase as a target detection object.

The invention discloses shape memory hemostasis and bone repair integrated frozen gel as well as a preparation method and application thereof. Natural macromolecular polysaccharide, inorganic nanofibers with bone repair activity and a cross-linking agent are added in the preparation process of the frozen gel, and the shape memory frozen gel with a double-network structure is formed through Schiff base reaction and electrostatic interaction. The prepared frozen gel has excellent liquid absorption capacity and mechanical strength, shows enhanced procoagulant activity, shows good cytocompatibility, and can enhance the activity of alkaline phosphatase and promote the formation of calcium nodules. Therefore, the material has rapid hemostasis and osteogenesis activity, and has a wide clinical application prospect.

The application discloses a preparation method of an ITO / Au@CdS / ALP photoelectrochemical sensor for rapidly detecting organophosphorus pesticides, which comprises the following steps: preparing Au@CdS NPs, and modifying alkaline phosphatase on the surface of the Au@CdS NPs to construct the sensor; the application is different from traditional photoelectrochemical sensors, which detect target substances by causing changes in system impedance or by introducing (removing) photoelectrochemical materials, but changes the intensity of photoelectric signals by changing the content of electron donors in the system; the preparation method is simple in operation, does not need a complex modification process, has good reproducibility and stability, and can rapidly, sensitively and accurately detect organophosphorus pesticides.

The invention discloses a preparation method of an ITO / Au (at) CdS (at) ALP photoelectrochemical sensor for rapidly detecting organophosphorus pesticides. The method comprises the following steps: preparing Au (at) CdS NPs, and modifying the surface of the Au (at) CdS NPs with alkaline phosphatase to construct the sensor; different from a traditional photoelectrochemical sensor, a target substance is detected by causing change of system impedance or introducing (removing) a photoelectrochemical material, the intensity of a photoelectric signal is changed by changing the content of an electron donor in a system, and the preparation method is simple to operate, does not need a complex modification process, and is suitable for large-scale production. The method has good reproducibility and stability, and can rapidly, sensitively and accurately detect the organophosphorus pesticide.

The invention discloses a preparation method and application of soybean protein with an optimal calcium-phosphorus ratio. The preparation method comprises the following steps: firstly, adding soybean meal into deionized water, adjusting the pH value to 7.5-8.0 by using a sodium hydroxide solution, stirring, centrifuging, taking supernatant, adjusting the pH value to 4.5 by using a hydrochloric acid solution, standing, centrifuging, and taking precipitate; then stirring and dispersing the precipitate in deionized water, adjusting the pH value to 7 by using a sodium hydroxide solution, and performing pulsed electric field treatment to obtain a premix; adding a compound enzyme I composed of neutral protease, bromelain, trypsin and fig protease into the premix, carrying out first enzymolysis, enzyme deactivation and homogenization treatment, adding a compound enzyme II composed of neutral phytase and alkaline phosphatase, and carrying out second enzymolysis, enzyme deactivation and pulsed electric field treatment to obtain an enzyme treatment product; and finally, adding magnetic chitosan microspheres into an enzyme treatment product, carrying out adsorption treatment and magnetic separation to remove the chitosan microspheres, and carrying out vacuum freeze drying to obtain the soybean protein. The calcium-phosphorus ratio of the obtained soybean protein is the best for human bodies, efficient absorption of calcium by the human bodies is facilitated, and the soybean protein can be used for food and beverage processing and is wide in application prospect.

The application belongs to the technical field of detection, and relates to a human papilloma virus detection reagent and application thereof. The human papilloma virus detection reagent comprises human papilloma virus antigen coated magnetic microparticle reagent and alkaline phosphatase labeled human papilloma virus antibody reagent. The human papilloma virus antigen coated magnetic microparticle is sealed by a sealing agent which is mixed by 2-10wt% PEG6000 solution, DB1130 and 0.1-1wt% gelatin solution. The system for detecting human papilloma virus antibody adopted by the application fills the gap of domestic HPV chemiluminescence antibody detection, and has high sensitivity, accuracy and clinical value.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention belongs to the technical field of metal modified biochar, and relates to manganese-rich silicon modified biochar, a preparation method thereof and application of the manganese-rich silicon modified biochar as oxidase. The manganese-silicon-rich modified charcoal is of a porous net structure and comprises elements of Zn, Si, S, P, O, N, Mn, Mg, K, Fe, Ca and Al, the Mn element is evenly distributed in the manganese-silicon-rich modified charcoal with the mass fraction larger than 45% and exists in the form of spinel type Mn3O4, the Ca element exists in the form of CaCO3, and the Si element exists in the form of SiO2. The manganese-silicon-rich modified charcoal has oxidase-like activity, can directly oxidize a specific organic substrate, does not need H2O2 participation, has good chemical stability and catalytic activity, can preserve efficient catalysis in a complex environment, and can be used as oxidase in detection of L-ascorbic acid or alkaline phosphatase.

The invention relates to a meadow alkaline halophilic bacterium LH-SA-1 capable of absorbing sodium and reducing salt and alkali as well as a saline-alkali soil remediation microbial inoculum and application of the meadow alkaline halophilic bacterium LH-SA-1. The LH-SA-1 is separated from severe saline alkali soil in Jilin Baicheng, the classification name of the LH-SA-1 is Alkalihalophilus lindianensis, and the preservation number of the LH-SA-1 is CGMCC (China General Microbiological Culture Collection Center) No. 32707. The LH-SA-1 can tolerate 3%-45% of various salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate and a strong alkali environment with the pH value of 9-12. The granular saline-alkali soil remediation microbial inoculum is prepared through composite sodium ion induced fermentation and double-membrane coupling concentration technologies in combination with a multifunctional carrier, the bacterial quantity is larger than or equal to 200 * 10 < 8 > CFU / g, and the catalase activity is larger than or equal to 130000 U / g. The microbial agent is applied to soda saline-alkali soil or coastal composite saline-alkali soil in northeast China, has multiple functions of remarkably reducing the saline-alkali content of the soil, improving the porosity of the soil, improving the activity of enzymes such as catalase, peroxidase, urease and alkaline phosphatase in the soil, improving the content of indoleacetic acid in the soil and the like, and provides an efficient and low-cost biological solution for saline-alkali remediation.

The invention provides a traditional Chinese medicine monomer composition for preventing and treating glucocorticoid osteoporosis and hypertension. The composition comprises the following active ingredients: beta ecdysterone, pinoresinol diglucoside and syringin according to a molar concentration combination ratio of 1: 1: 2. The invention also discloses application of the traditional Chinese medicine monomer composition in preparation of medicines or health-care foods for preventing and treating glucocorticoid osteoporosis and hypertension. The traditional Chinese medicine monomer composition can effectively improve the spine mineralized bone mass, the spine bone density, the alkaline phosphatase activity, the blood vessel diameter and the blood pressure resistance of a zebrafish model with the osteoporosis and hypertension co-disease induced by glucocorticoid. In addition, by inhibiting oxidative stress and cell apoptosis caused by glucocorticoid, bone formation is promoted, angiogenesis is promoted, and then the osteoporosis and angiotension state is improved. The traditional Chinese medicine monomer composition can effectively prevent and relieve osteoporosis and vascular adverse reactions caused by glucocorticoid, and has good application and development prospects.

The application provides a strain of mineralized soil organic phosphorus, Domibacillus indicus D99, and application of the strain in soil organic phosphorus mineralization, growth promotion of phosphorus solubilizing bacteria and plant growth promotion. The application first shows the function and role of Domibacillus indicus in soil organic phosphorus mineralization and plant growth promotion, and has important value for development of phosphorus solubilizing bacteria fertilizer and other microbial fertilizers. The strain of Domibacillus indicus D99 provided by the application has the ability of dissolving soil organic phosphorus, the acid phosphatase activity is 17.32, the alkaline phosphatase activity is 1.3, can promote plant growth, and improves the aboveground biomass by 50-115.

The application relates to a hepcidin chemiluminescence immunoassay kit and a non-diagnostic method for detecting hepcidin, the kit comprising a calibrator, a quality control, an integrated bifunctional antibody reagent, a magnetic particle reagent, a cleaning solution and a chemiluminescence substrate solution; the integrated bifunctional antibody reagent is a buffer containing HEP-specific antibodies covalently connected with fluorescein isothiocyanate and alkaline phosphatase through pH-sensitive connecting bonds; the magnetic particle reagent is a magnetic microsphere suspension coated with a goat anti-fluorescein isothiocyanate antibody; the cleaning solution is a buffer containing a surfactant composition, and the surfactant composition comprises nonionic surfactants, zwitterionic surfactants and high-molecular nonionic surfactants. The hepcidin chemiluminescence immunoassay kit has the effects of ultra-low background noise, ultra-high sensitivity, ultra-wide linear range and simplified operation of end users by means of the integrated bifunctional antibody reagent and the surfactant composition cleaning solution.

The application belongs to the technical field of biological detection, and particularly relates to a detection system of foodborne pathogenic bacteria and application thereof. The detection system of foodborne pathogenic bacteria comprises a composite response material, a hyaluronic acid hydrogel containing L-ascorbic acid-2-phosphoric acid trisodium salt and a bacteriophage; the composite response material comprises ZIF-90 nanoparticles and hyaluronidase and alkaline phosphatase encapsulated in the ZIF-90 nanoparticles. The application constructs a dual-mode paper-based visual detection system based on the combination of specific recognition of a bacteriophage and ZIF-90 response release mechanism, and realizes high-specificity detection of target pathogenic bacteria. Compared with the defects that nucleic acid amplification or immunological methods are difficult to distinguish live bacteria from dead bacteria, the application only produces a signal response to live bacteria that can be infected by a bacteriophage, and significantly improves the authenticity and reliability of detection results.

The present invention relates to a bacteria-responsive hemostatic and antibacterial cotton fabric and a preparation method thereof. By utilizing amino-modified phosphate ester, the oxidized cotton fabric and the amino-rich cationic hemostatic and antibacterial agent are connected together, thereby realizing the hemostatic and antibacterial functional finishing of the cotton fabric. The selected antibacterial agent has better biosafety than the commonly used nanosilver. The obtained cotton fabric can be applied to the medical and clothing fields and has bioresponsiveness: it has inherent antibacterial and hemostatic ability under normal use environment, and chemical grafting ensures sufficient wash resistance; and when the body is colonized by a large number of bacteria, the bacteria-responsive phosphate bond is catalyzed by the alkaline phosphatase secreted by the bacteria to break, releasing the free antibacterial agent, which enters the tissue to kill bacteria, thereby promoting rapid recovery from infectious diseases.

The invention discloses a preparation process of bovine prostate dry powder. The preparation process comprises the following steps: pretreating raw materials; preparing homogenate; freezing and thawing treatment; filtering and separating; carrying out alkaline protease enzymolysis; performing enzymolysis with acid protease; performing activation treatment; performing primary acetone precipitation; carrying out secondary and tertiary acetone precipitation; drying and crushing; the moistening is balanced; the invention relates to the technical field of bioengineering, through a three-stage system of freezing and thawing, double-stage enzymolysis and activation, cell structures are damaged by freezing and thawing, proteins are fully decomposed by double-stage enzymolysis, release of active substances is enhanced in the activation step, and the retention rate of core components such as alkaline phosphatase is effectively increased; after water-soluble impurities are removed step by step through acetone gradient precipitation and a moistening phase is balanced and optimized in a phase state, fat-soluble impurities are further removed through ethanol repeated precipitation, the product purity is improved, and the residual amount of an organic solvent is effectively reduced.

The present invention relates to the field of microbial engineering technology, and specifically discloses a composite microbial agent for controlling cyanobacterial blooms. The Bacillus velezinoff provided by the present invention destroys algal cells and inhibits the activity of related biological enzymes by secreting heterocyclic algaecidal active substances, while causing the leakage of cell contents (such as algal toxins, polysaccharides, and lipids); the extracellular enzyme system (such as alkaline phosphatase and β-glucosidase) of Sphingomonas decomposes free algal toxins into low-toxic linear peptide segments, while hydrolyzing the β-1,4-glycosidic bonds in EPS, converting algal organic fragments into small-molecule soluble substances, and providing substrates for subsequent metabolism of Rhodococcus; the lipase and esterase secreted by Rhodococcus can hydrolyze triglycerides and wax components, and convert fatty acids into energy and biosurfactants through a β-oxidation pathway, thereby enhancing the hydrophilicity of algal fragments and promoting overall degradation efficiency.

The invention relates to the technical field of microbial fertilizers, in particular to a compound microbial fertilizer for deacidification and recarburization of red and yellow soil and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer is prepared from the following raw materials in parts by mass: 40 to 60 parts of livestock manure, 30 to 50 parts of soybean meal, 1 to 3 parts of lime powder, 1 to 3 parts of potassium mineral powder, 1 to 2 parts of bentonite, 1 to 3 parts of compound trace element agent, 10 to 30 parts of bamboo charcoal powder, 5 to 15 parts of corn starch, 5 to 15 parts of sodium pyrophosphate, 1 to 2 parts of alkaline phosphatase, 1 to 3 parts of dopamine and 1 to 5 parts of microbial agent. The obtained compound microbial fertilizer not only can effectively adjust the acid environment of red and yellow soil and continuously increase the carbon reserve of the soil, but also can provide comprehensive guarantee for plant growth by improving the physical structure and biological activity of the soil, and finally achieves the target of increasing the yield and improving the quality of crops; and the preparation method has the advantages of easily available raw materials, feasible process and good application prospect.