110 results about "Alkaline phosphatase" patented technology

Zeolite / clay / phosphate catalysts can be prepared by a process wherein a composition of zeolite-clay-phosphate is brought to a pH level of about 7.0 to about 14.0. The resulting slurry is then age reacted for about 0.5 to about 24 hours. Thereafter the slurry is dried to produce a zeolite / clay / phosphate catalyst particles that are particularly characterized by their high levels of zeolite stability.

The invention relates to a dental implant and a preparation method of a bioactive antibacterial surface of the dental implant. The dental implant is characterized in that medical titanium or a titanium alloy is taken a substrate; a nanotube morphology is constructed on the surface of the substrate through anodic oxidation; the surface of the substrate is uniformly coated with a polydopamine bionic membrane layer formed by auto-polymerization; the dental implant with the bioactive antibacterial surface provided with in-situ grafted silver nanoparticles is obtained by means of the adsorption and reduction capabilities of a polydopamine surface for silver ions. By adopting the dental implant, a uniform discrete nanotube oxide layer is formed in situ on the surface of the titanium substrate to be taken as a storage carrier of a large quantity of antibacterial silver particles, and the bioactive antibacterial surface which grows in situ is prepared through the steps of surface dopamine auto-polymerization and in-situ soaking and reduction and the like, so that the implant surface has more durable antibacterial property, the hydrophilic performance and bioactivity of the surface are improved, the alkaline phosphatase activity of osteoblast on the implant surface is increased, the differentiation of the osteoblast is facilitated, the infection risk of the implant after the implant is implanted is lowered, and the implanting success rate is increased.

The present invention provides methods of improving the osteogenic and / or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The treatment or condition may alter the structure of the bone matrix and / or cleave one or more specific proteins. Cleavage may generate peptides or protein fragments that have osteoinductive, osteogenic, or chondrogenic activity. Preferred treatments include collagenase and various other proteases. The invention further provides improved bone and cartilage matrix compositions that have been prepared according to the inventive methods and methods of treatment using the compositions. The invention further provides methods of preparing, testing, and using the improved bone matrix compositions. On a assay comprises exposing relatively undifferentiated mesenchymal cells to a bone matrix composition and measuring expression of a marker characteristic of osteoblast or chondrocyte lineage(s). Increased expression of the marker relative to the level of the marker in cells that have been exposed to a control matrix (e.g., an inactivated or untreated matrix) indicates that the treatment or condition increased the osteogenic and / or chondrogenic activity of the bone matrix. Suitable cells include C2C12 cells. A suitable marker is alkaline phosphatase. The inventive methods increase the osteogenic and / or chondrogenic activity of human DBM when tested using this assay system.

Described herein are reprogramming techniques allowing for production of mammary-derived iPSCs (“m-iPSCs”). The m-iPSCs described herein exhibit all the hallmarks of stem cell identity including round cluster, bright colony morphology, clonal expansion, and pluripotent marker expression (alkaline phosphatase expression, Oct-4, nanog, etc.) Further refined techniques allow for generation of m-iPSCs under essentially defined conditions.

The invention discloses a fusion gene fragment rolB-FGFs, which consists of a rolB gene and an FGFs gene that is designed according to the preference of a plant codon, wherein a PIM gene without the antibiotic marker and a human secreted alkaline phosphatase signal peptide (SEAP) gene are introduced; a plant secreted binary expression vector without the antibiotic marker is constructed; the expression vector is transformed into agrobacterium rhizogene; by taking the plant as the host and adopting a hairy root system to express the FGFs, the active FGFs can be purified from a hairy root and culture solution respectively, and the FGFs can be prepared industrially, thereby improving the inductivity, the expressed protein amount, the yield and the stability of the hairy root; and no antibiotic marker exists in the method, thereby protecting the environment. The ginseng hairy root containing the FGFs prepared by taking the ginseng as the host has the double pharmacological effects of the ginseng and the FGFs, thereby being a better health-care product or medicine; in addition, the ginseng hairy root is used for the development and the application of the functional food and medicine and has wide prospect.

The invention relates to an antibody chip for screening and diagnosing compositae plant virus diseases, and a kit and a method of the antibody chip. The antibody chip for screening and diagnosing compositae plant virus diseases is mainly characterized by comprising a base membrane and specific capture antibodies (primary antibodies) which are respectively pointed on the base membrane and used for capturing seven types of viruses with harm to the compositae plant, wherein the antibodies (primary antibodies) for capturing seven types of viruses have resistance to bean yellow mosaic virus, resistance to arabis mosaic virus, resistance to curtobacterium flaccumfaciens virus, resistance to cucumber mosaic virus, resistance to tobacco rattle virus, resistance to tobacco ringspot virus and resistance to tomato spotted wilt virus; three positive quality control points P, IgG pointed to be alkaline phosphatase mark, three negative quality control points N and a carbonate buffer solution pointed to reach a pH being 9.2-9.8 are also encapsulated on the base membrane. The antibody chip for screening and diagnosing compositae plant virus diseases has the advantages that the antibody chip for detecting a plurality of viruses of the compositae plant is successfully built, so that comprehensive detection is carried out on a plurality of pathogens at a high flux.

The invention discloses a medical implant and a preparation method thereof. The method comprises the following steps: firstly, constructing a special memory deformation high polymer material with pores, then fixing the special memory deformation high polymer material on the surface of an implant body, and carrying out volume expansion on the memory deformation high polymer material under illumination or temperature stimulation to obtain the degradable expansion implant. Compared with an existing medical implant, the implant is jointly composed of an outer-layer deformable material and an innerimplant body, and after the implant is implanted into sclerotin, the outer-layer high polymer material of the implant expands in a manual regulation and control mode, so that the high initial stability of the implant is achieved. Due to the degradability and the growth factor carrying capacity of the material, the synthesis of bone cell alkaline phosphatase and the deposition of calcium-containing minerals can be effectively promoted, and osteogenesis is induced, so that the rapid and effective osseointegration effect of the implant is finally realized.