48 results about "Alkaline phosphatase" patented technology

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention discloses a nano-material-based nitrogen-phosphorus-potassium compound fertilizer as well as a preparation method and application thereof, and belongs to the field of plant nutrition and soil science research. According to the invention, the nano biochar prepared through nano grinding after pyrolysis of the rice straw is directly mixed with three chemical components, namely the urea, the ammonium dihydrogen phosphate and the potassium chloride, and the sodium bentonite, and the key points of the effects are triple effects of promoting early growth and rapid ripening, enhancing tillering and advancing heading. After the nano biochar is matched with balanced NPK nutrition (such as NPK-II), the activity level of soil key enzymes, including catalase (CAT), alkaline phosphatase (AKP), urease (UE), sucrase (SC) and the like, is remarkably improved. Besides, the composite particles are constructed through the cooperation of the nano-charcoal and the bentonite, and a low-temperature drying process is adopted, so that moisture absorption and caking can be inhibited, and the storage stability and field application performance of the fertilizer are remarkably improved.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

A method for improving saline-alkaline soil using Salvia miltiorrhiza combined with biochar, comprising the following steps: S1. Soil preparation: applying harmless treatment organic fertilizer as a base fertilizer, evenly applying biochar according to a proportion, plowing and tilling the soil, and, according to planting density and topographical conditions, planning ridge spacing and ridge width of double high ridges; S2. Salvia miltiorrhiza planting: performing Salvia miltiorrhiza seedling transplantation using a seedling transplantation method and hole planting means, digging holes on ridge surfaces, trimming overly long parts of seedling roots, planting seedlings vertically, and covering with soil; S3. field management after transplanting: performing fertilization, weeding, and top dressing management. The present method for improving saline-alkaline soil using Salvia miltiorrhiza cultivation combined with biochar application reduces soil pH and total salt content, increases soil sucrose enzyme activity, increases soil alkaline phosphatase activity, and alters soil microbiome structure.

The invention relates to the technical field of kits, in particular to a magnetic particle chemiluminiscence detection kit for insulin and a preparation method of the magnetic particle chemiluminiscence detection kit for insulin, specifically, a reagent M, a reagent R2 and a reagent R1 are used for forming the kit, and the reagent M is a magnetic particle working solution containing streptavidin magnetic beads; the reagent R2 is an enzyme-labeled working solution containing an insulin antibody labeled by alkaline phosphatase; the reagent R1 is a working solution containing a biotin-labeled insulin monoclonal antibody. Through verification, according to the chemiluminescence kit for insulin provided by the invention, the test sensitivity reaches 2.00 mu IU / mL, the test repeatability for high and low value samples is less than 3%, and the linear correlation with Rogowski reaches 0.98 within the concentration range of 2.00-450 mu IU / mL. The invention also provides a preparation method of the insulin chemiluminescence kit, the production cycle is greatly shortened, the batch difference is easy to control, and the production work of enterprises is facilitated.

The application provides a skin interstitial fluid miRNA multicolor visualization semi-quantitative detection method, which comprises the following steps: incubating a skin interstitial fluid sample with magnetic nano probes of surface immobilized hairpin probes H1, opening H1 and triggering a subsequent hybridization chain reaction of hairpin probes H2 and H3 in the presence of target miRNA, and constructing a biotin-rich DNA complex on the surface of the magnetic beads; performing enzyme labeling by using an alkaline phosphatase-streptavidin coupling compound, and catalyzing ascorbic acid phosphate to generate ascorbic acid potassium iodate, so that the color of the solution changes, and high-sensitivity detection of the target miRNA is realized. The multicolor visualization detection method can produce near-full-spectrum color changes by combining the gold nano double-cone local surface plasmon resonance property change, and is beneficial to improving the portability of the sensor and the accuracy of the visual analysis.

The invention discloses shape memory hemostasis and bone repair integrated frozen gel as well as a preparation method and application thereof. Natural macromolecular polysaccharide, inorganic nanofibers with bone repair activity and a cross-linking agent are added in the preparation process of the frozen gel, and the shape memory frozen gel with a double-network structure is formed through Schiff base reaction and electrostatic interaction. The prepared frozen gel has excellent liquid absorption capacity and mechanical strength, shows enhanced procoagulant activity, shows good cytocompatibility, and can enhance the activity of alkaline phosphatase and promote the formation of calcium nodules. Therefore, the material has rapid hemostasis and osteogenesis activity, and has a wide clinical application prospect.

The application discloses a preparation method of an ITO / Au@CdS / ALP photoelectrochemical sensor for rapidly detecting organophosphorus pesticides, which comprises the following steps: preparing Au@CdS NPs, and modifying alkaline phosphatase on the surface of the Au@CdS NPs to construct the sensor; the application is different from traditional photoelectrochemical sensors, which detect target substances by causing changes in system impedance or by introducing (removing) photoelectrochemical materials, but changes the intensity of photoelectric signals by changing the content of electron donors in the system; the preparation method is simple in operation, does not need a complex modification process, has good reproducibility and stability, and can rapidly, sensitively and accurately detect organophosphorus pesticides.

The application belongs to the technical field of detection, and relates to a human papilloma virus detection reagent and application thereof. The human papilloma virus detection reagent comprises human papilloma virus antigen coated magnetic microparticle reagent and alkaline phosphatase labeled human papilloma virus antibody reagent. The human papilloma virus antigen coated magnetic microparticle is sealed by a sealing agent which is mixed by 2-10wt% PEG6000 solution, DB1130 and 0.1-1wt% gelatin solution. The system for detecting human papilloma virus antibody adopted by the application fills the gap of domestic HPV chemiluminescence antibody detection, and has high sensitivity, accuracy and clinical value.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention belongs to the technical field of metal modified biochar, and relates to manganese-rich silicon modified biochar, a preparation method thereof and application of the manganese-rich silicon modified biochar as oxidase. The manganese-silicon-rich modified charcoal is of a porous net structure and comprises elements of Zn, Si, S, P, O, N, Mn, Mg, K, Fe, Ca and Al, the Mn element is evenly distributed in the manganese-silicon-rich modified charcoal with the mass fraction larger than 45% and exists in the form of spinel type Mn3O4, the Ca element exists in the form of CaCO3, and the Si element exists in the form of SiO2. The manganese-silicon-rich modified charcoal has oxidase-like activity, can directly oxidize a specific organic substrate, does not need H2O2 participation, has good chemical stability and catalytic activity, can preserve efficient catalysis in a complex environment, and can be used as oxidase in detection of L-ascorbic acid or alkaline phosphatase.

The invention relates to a meadow alkaline halophilic bacterium LH-SA-1 capable of absorbing sodium and reducing salt and alkali as well as a saline-alkali soil remediation microbial inoculum and application of the meadow alkaline halophilic bacterium LH-SA-1. The LH-SA-1 is separated from severe saline alkali soil in Jilin Baicheng, the classification name of the LH-SA-1 is Alkalihalophilus lindianensis, and the preservation number of the LH-SA-1 is CGMCC (China General Microbiological Culture Collection Center) No. 32707. The LH-SA-1 can tolerate 3%-45% of various salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate and a strong alkali environment with the pH value of 9-12. The granular saline-alkali soil remediation microbial inoculum is prepared through composite sodium ion induced fermentation and double-membrane coupling concentration technologies in combination with a multifunctional carrier, the bacterial quantity is larger than or equal to 200 * 10 < 8 > CFU / g, and the catalase activity is larger than or equal to 130000 U / g. The microbial agent is applied to soda saline-alkali soil or coastal composite saline-alkali soil in northeast China, has multiple functions of remarkably reducing the saline-alkali content of the soil, improving the porosity of the soil, improving the activity of enzymes such as catalase, peroxidase, urease and alkaline phosphatase in the soil, improving the content of indoleacetic acid in the soil and the like, and provides an efficient and low-cost biological solution for saline-alkali remediation.

The invention provides a traditional Chinese medicine monomer composition for preventing and treating glucocorticoid osteoporosis and hypertension. The composition comprises the following active ingredients: beta ecdysterone, pinoresinol diglucoside and syringin according to a molar concentration combination ratio of 1: 1: 2. The invention also discloses application of the traditional Chinese medicine monomer composition in preparation of medicines or health-care foods for preventing and treating glucocorticoid osteoporosis and hypertension. The traditional Chinese medicine monomer composition can effectively improve the spine mineralized bone mass, the spine bone density, the alkaline phosphatase activity, the blood vessel diameter and the blood pressure resistance of a zebrafish model with the osteoporosis and hypertension co-disease induced by glucocorticoid. In addition, by inhibiting oxidative stress and cell apoptosis caused by glucocorticoid, bone formation is promoted, angiogenesis is promoted, and then the osteoporosis and angiotension state is improved. The traditional Chinese medicine monomer composition can effectively prevent and relieve osteoporosis and vascular adverse reactions caused by glucocorticoid, and has good application and development prospects.

The application provides a strain of mineralized soil organic phosphorus, Domibacillus indicus D99, and application of the strain in soil organic phosphorus mineralization, growth promotion of phosphorus solubilizing bacteria and plant growth promotion. The application first shows the function and role of Domibacillus indicus in soil organic phosphorus mineralization and plant growth promotion, and has important value for development of phosphorus solubilizing bacteria fertilizer and other microbial fertilizers. The strain of Domibacillus indicus D99 provided by the application has the ability of dissolving soil organic phosphorus, the acid phosphatase activity is 17.32, the alkaline phosphatase activity is 1.3, can promote plant growth, and improves the aboveground biomass by 50-115.

The application relates to a hepcidin chemiluminescence immunoassay kit and a non-diagnostic method for detecting hepcidin, the kit comprising a calibrator, a quality control, an integrated bifunctional antibody reagent, a magnetic particle reagent, a cleaning solution and a chemiluminescence substrate solution; the integrated bifunctional antibody reagent is a buffer containing HEP-specific antibodies covalently connected with fluorescein isothiocyanate and alkaline phosphatase through pH-sensitive connecting bonds; the magnetic particle reagent is a magnetic microsphere suspension coated with a goat anti-fluorescein isothiocyanate antibody; the cleaning solution is a buffer containing a surfactant composition, and the surfactant composition comprises nonionic surfactants, zwitterionic surfactants and high-molecular nonionic surfactants. The hepcidin chemiluminescence immunoassay kit has the effects of ultra-low background noise, ultra-high sensitivity, ultra-wide linear range and simplified operation of end users by means of the integrated bifunctional antibody reagent and the surfactant composition cleaning solution.

The application belongs to the technical field of biological detection, and particularly relates to a detection system of foodborne pathogenic bacteria and application thereof. The detection system of foodborne pathogenic bacteria comprises a composite response material, a hyaluronic acid hydrogel containing L-ascorbic acid-2-phosphoric acid trisodium salt and a bacteriophage; the composite response material comprises ZIF-90 nanoparticles and hyaluronidase and alkaline phosphatase encapsulated in the ZIF-90 nanoparticles. The application constructs a dual-mode paper-based visual detection system based on the combination of specific recognition of a bacteriophage and ZIF-90 response release mechanism, and realizes high-specificity detection of target pathogenic bacteria. Compared with the defects that nucleic acid amplification or immunological methods are difficult to distinguish live bacteria from dead bacteria, the application only produces a signal response to live bacteria that can be infected by a bacteriophage, and significantly improves the authenticity and reliability of detection results.

The present invention relates to a bacteria-responsive hemostatic and antibacterial cotton fabric and a preparation method thereof. By utilizing amino-modified phosphate ester, the oxidized cotton fabric and the amino-rich cationic hemostatic and antibacterial agent are connected together, thereby realizing the hemostatic and antibacterial functional finishing of the cotton fabric. The selected antibacterial agent has better biosafety than the commonly used nanosilver. The obtained cotton fabric can be applied to the medical and clothing fields and has bioresponsiveness: it has inherent antibacterial and hemostatic ability under normal use environment, and chemical grafting ensures sufficient wash resistance; and when the body is colonized by a large number of bacteria, the bacteria-responsive phosphate bond is catalyzed by the alkaline phosphatase secreted by the bacteria to break, releasing the free antibacterial agent, which enters the tissue to kill bacteria, thereby promoting rapid recovery from infectious diseases.

The invention discloses a preparation process of bovine prostate dry powder. The preparation process comprises the following steps: pretreating raw materials; preparing homogenate; freezing and thawing treatment; filtering and separating; carrying out alkaline protease enzymolysis; performing enzymolysis with acid protease; performing activation treatment; performing primary acetone precipitation; carrying out secondary and tertiary acetone precipitation; drying and crushing; the moistening is balanced; the invention relates to the technical field of bioengineering, through a three-stage system of freezing and thawing, double-stage enzymolysis and activation, cell structures are damaged by freezing and thawing, proteins are fully decomposed by double-stage enzymolysis, release of active substances is enhanced in the activation step, and the retention rate of core components such as alkaline phosphatase is effectively increased; after water-soluble impurities are removed step by step through acetone gradient precipitation and a moistening phase is balanced and optimized in a phase state, fat-soluble impurities are further removed through ethanol repeated precipitation, the product purity is improved, and the residual amount of an organic solvent is effectively reduced.

The present invention relates to the field of microbial engineering technology, and specifically discloses a composite microbial agent for controlling cyanobacterial blooms. The Bacillus velezinoff provided by the present invention destroys algal cells and inhibits the activity of related biological enzymes by secreting heterocyclic algaecidal active substances, while causing the leakage of cell contents (such as algal toxins, polysaccharides, and lipids); the extracellular enzyme system (such as alkaline phosphatase and β-glucosidase) of Sphingomonas decomposes free algal toxins into low-toxic linear peptide segments, while hydrolyzing the β-1,4-glycosidic bonds in EPS, converting algal organic fragments into small-molecule soluble substances, and providing substrates for subsequent metabolism of Rhodococcus; the lipase and esterase secreted by Rhodococcus can hydrolyze triglycerides and wax components, and convert fatty acids into energy and biosurfactants through a β-oxidation pathway, thereby enhancing the hydrophilicity of algal fragments and promoting overall degradation efficiency.

The invention relates to the technical field of microbial fertilizers, in particular to a compound microbial fertilizer for deacidification and recarburization of red and yellow soil and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer is prepared from the following raw materials in parts by mass: 40 to 60 parts of livestock manure, 30 to 50 parts of soybean meal, 1 to 3 parts of lime powder, 1 to 3 parts of potassium mineral powder, 1 to 2 parts of bentonite, 1 to 3 parts of compound trace element agent, 10 to 30 parts of bamboo charcoal powder, 5 to 15 parts of corn starch, 5 to 15 parts of sodium pyrophosphate, 1 to 2 parts of alkaline phosphatase, 1 to 3 parts of dopamine and 1 to 5 parts of microbial agent. The obtained compound microbial fertilizer not only can effectively adjust the acid environment of red and yellow soil and continuously increase the carbon reserve of the soil, but also can provide comprehensive guarantee for plant growth by improving the physical structure and biological activity of the soil, and finally achieves the target of increasing the yield and improving the quality of crops; and the preparation method has the advantages of easily available raw materials, feasible process and good application prospect.

The application discloses a cucumber mosaic virus photoelectric chemical sensor and a detection method thereof, and belongs to the technical field of photoelectric chemical sensors.The existing PEC sensor has the problems of low photoelectric conversion efficiency and lack of efficient signal amplification strategy.The sensor provided by the application takes a screen-printed electrode as a substrate, a three-dimensional flower-shaped ZnIn2S4 nanomaterial is used as a light active layer to modify a working electrode, and a basic phosphatase-mediated enzymatic biological catalytic precipitation signal amplification system is matched;the detection method adopts a sandwich immunization mode to capture viruses, catalyzes the generation of BCP precipitation to shield the transmission of photo-generated carriers, and quantitatively detects through photocurrent attenuation, the linear range of the detection is 0.001-200 ng / mL, and the detection limit is 0.001 ng / mL, so that the cucumber mosaic virus can be rapidly screened in a field.

The application belongs to the technical field of biomedicine, and specifically discloses a squamous cell carcinoma antigen determination kit and application, which comprises anti-reagent A, anti-reagent B and magnetic micro-particle reagent, wherein the anti-reagent A is a biotin-labeled squamous cell carcinoma antigen antibody; the anti-reagent B is an alkaline phosphatase-labeled squamous cell carcinoma antigen antibody; the squamous cell carcinoma antigen determination kit can effectively detect the concentration of squamous cell carcinoma antigen (SCC) in serum and plasma samples, and is mainly used for the treatment monitoring of cervical cancer, non-small cell carcinoma and the like, and has a wide application prospect.

The invention relates to an immortalized cell line of human dermal papilla cells as well as a construction method and application of the immortalized cell line. The human dermal papilla cells are named as DYZ-20, the immortalized cell line is preserved in the China Center for Type Culture Collection on July 2, 2025, survival is detected, and the preservation number is CCTCC NO: C2025204. The immortalized cell line of the human dermal papilla cells can normally express alkaline phosphatase and a dermal papilla characteristic gene, and hair can grow after the immortalized cell line is transplanted into mouse skin.

The invention relates to an insulin-like growth factor I quantitative determination kit and a preparation method thereof. Specifically, the insulin-like growth factor I detection kit comprises: 1) a lysate; 2) a reagent buffer solution; 3) coupling the anti-insulin-like growth factor I antibody with alkaline phosphatase; and 4) a biotin-labeled anti-insulin-like growth factor I antibody. The insulin-like growth factor I detection kit disclosed by the invention is good in stability, strong in specificity and good in precision.

The invention provides an external traditional Chinese medicine composition with a bacteriostatic effect and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition comprises 20-40 parts of pepper, 20-40 parts of fructus cnidii, 10-30 parts of ligusticum, 5-15 parts of pogostemon cablin, 20-40 parts of schizonepeta, 10-30 parts of senecio scandens and 20-40 parts of mint. A response surface experiment design is adopted to optimize an extraction process of traditional Chinese medicinal materials and medicine residues, and the content of effective components such as total flavonoids in the extract is increased; the oil-soluble matrix and the water-soluble matrix are selected, so that the water-soluble medicine components and the oil-soluble medicine components can be better mixed, and the stability is high. The traditional Chinese medicine composition disclosed by the invention can inhibit the formation of bacterial cell membranes and destroy the structures of the bacterial cell membranes, and the permeability of the cell membranes is improved, so that macromolecular substances and proteins are leaked; the intracellular ATP content is reduced, and the extracellular alkaline phosphatase is increased, so that the growth of bacteria is finally inhibited or the bacteria are killed. According to the extraction process, effective components of the medicine are extracted to the maximum extent, and efficient utilization of the medicinal materials is guaranteed.

The invention provides a GDF-15 (growth differentiation factor-15) magnetic particle chemiluminescence immunoassay quantitative detection kit, which belongs to the field of in vitro diagnosis, and comprises a magnetic particle suspension, a biotin-labeled GDF-15 capture antibody solution, an alkaline phosphatase-labeled GDF-15 detection antibody solution, a calibrator, a chemiluminescence solution and the like. The kit provided by the invention has the advantages of rapidness and simplicity in operation, high sensitivity and repeatability, large measurement range and good stability, and can make up for the defects of the existing detection method.

The present invention provides a double-staining immunohistochemistry kit and its application, which relates to the field of immunohistochemistry. Among them, the double-staining immunohistochemistry kit includes: a fixative, a membrane-breaking agent, a primary antibody reagent, a secondary antibody reagent, and a staining agent. Among them, the primary antibody reagent includes a LEF1 antibody and a CD20 antibody. The secondary antibody reagent includes a secondary antibody polymer labeled with alkaline phosphatase AP and a secondary antibody polymer labeled with horseradish peroxidase HRP. The kit can obtain stained sample cells with complete cell morphology, has a simple process, can effectively improve the recognition rate of cell morphology, and improve the diagnostic accuracy rate.

This invention discloses a method for detecting organophosphorus pesticides using a self-cascaded Pt@Fe-CDs nanozyme, belonging to the field of chemical analysis and detection technology. This method synthesizes Pt@Fe-CDs nanozymes using iron-doped carbon dots (Fe-CDs) as a reducing agent and template. Pt@Fe-CDs simultaneously possess ascorbic acid oxidase-like and peroxidase-like activities. Under the catalysis of alkaline phosphatase, they hydrolyze L-ascorbic acid-2-phosphate trisodium salt to AA. Based on the fact that organophosphorus pesticides can inhibit ALP activity and hinder AA2P dephosphorylation, leading to a reduction in AA and thus causing changes in fluorescence and colorimetric signals, a simple and sensitive dual-signal fluorescence / colorimetric sensing platform for organophosphorus pesticides is constructed, with a detection limit of 0.28-0.53 ng / mL. The colorimetric and fluorescence biosensors established in this invention exhibit excellent performance in terms of sensitivity, specificity, and accuracy in actual samples.