Novel coronavirus SARS-CoV-2 S protein detection method

Abstract

The invention discloses a novel coronavirus SARS-CoV-2 S protein detection method. SARS-CoV-2 virus depends on spike protein on the surface to be combined with angiotensin converting enzyme 2 on the surface of a cell so as to enter the cell; according to the method, raw materials commonly used in chemiluminescence immunoassay and ACE2 are easy to purchase from the market, the biotin labeling and the alkaline phosphatase labeling are rapid, the reagent for SARS-CoV-2 S protein detection can be rapidly prepared, and the positive coincidence rate, the negative coincidence rate and the total coincidence rate are high compared with the SARS-CoV-2 fluorescence quantitative PCR result.

Description

technical field

[0001] The invention relates to a virus detection method, in particular to a novel coronavirus SARS-CoV-2 S protein detection method. Background technique

[0002] Viral antigen detection and immunological analysis methods usually use the double antibody sandwich method. Taking the detection of SARS-CoV N protein antigen as an example, the solid-phase end is SARS-CoV N protein-specific antibody, and the labeled end is SARS-CoV N protein-specific antibody. After adding the analyte and incubating, the SARS-CoV N protein in the analyte will combine with the SARS-CoV N protein-specific antibody at the solid phase end to form an immune complex, and at the same time, the SARS-CoV N protein-specific antibody at the label end will combine with the SARS-CoV N protein-specific antibody in the sample. - Different antigenic sites on the CoV N protein bind to form a labeled immune complex, and the unbound enzyme conjugates and other substances are removed by washing. By ...