170 results about "Chemiluminescence immunoassay" patented technology

This invention relates to the field of chemiluminescence immunoassay technology, specifically to a magnetic bead coating method and its application for improving the luminescence level of p-tau 217. This invention optimizes the process of coating p-tau 217 capture antibodies with carboxyl magnetic beads. By gradient adjustment of the input ratio of EDC and NHS, the optimal activator concentration is obtained to enhance the magnetic bead coupling activity, increase the antibody coupling efficiency, and thus improve the signal values ​​of antigen samples and clinical samples. This invention has broad application prospects for the development of highly sensitive detection of low-abundance proteins such as p-tau 217.

This invention provides a kit for detecting anti-peroxidase-1-IgG antibodies, comprising the antigen protein peroxiredoxin-1 (peroxidase-1), a solid-phase carrier, labeled antibody, antigen dilution buffer, sample dilution buffer, antibody dilution buffer, substrate chromogenic agent, washing buffer, standards, positive control, and negative control. This kit utilizes an indirect reaction principle combined with magnetic microparticle chemiluminescence immunoassay to detect anti-peroxidase-1-IgG antibodies in the serum of the test sample. This invention is the first to identify autoantibodies against the target antigen peroxidase-1 in the serum of patients with autoimmune nephrotic syndrome. The provided kit provides a basis for domestic and international research on the molecular mechanisms and clinical diagnosis and treatment of autoimmune nephrotic syndrome related to peroxidase-1 and peroxidase-1-IgG autoantibodies.

The application discloses a kind of chemiluminescence immunoassay analyzer cup arrangement systems, including vertically arranged support plate, support plate one side is equipped with hopper, the other side is equipped with reaction cup guide rail, the reaction cup guide rail includes the turning slide way that is connected in the lower end of front slide way, turning slide way is overall inclined arrangement, turning slide way includes waiting section, cup section and the transition section that is connected between waiting section and cup section, waiting section and cup section are straight slide way, wherein, waiting section is connected in the lower end of front slide way extension direction, transition section is arc slide way, transition section makes cup section and waiting section between having angle of intersection.The beneficial effects of the present application are: by being provided with turning slide way in the end of guide rail, so that the corresponding module in analyzer can be arranged along the width direction of guide rail, improve design flexibility, help to optimize equipment space utilization.

The invention relates to a preparation method of an 8-hydroxyguanine (8-Hydroxyguanine) detection kit based on a chemiluminescence immunoassay technology. The 8-Hydroxyguanine (8-Hydroxyguanine) detection kit is used for detecting 8-Hydroxyguanine. As 8-hydroxyguanine is small in molecular weight and poor in immunogenicity, when a corresponding antibody is prepared, firstly, a proper derivative site needs to be selected, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and high affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of an 8-hydroxyguanine hapten, preparation of an 8-hydroxyguanine complete antigen and an anti-8-hydroxyguanine antibody, a method for determining the concentration of 8-hydroxyguanine, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of 8-hydroxyguanine derivatives. The 8-hydroxyguanine derivative disclosed by the invention has a structure as shown in a formula (I),

The invention belongs to the technical field of chemiluminescence immunoassay, and particularly relates to application of a luminescence retarder in improving development quality of solid-phase membrane immunodetection. According to the invention, when the concentration of the delay agent is fixed, the time of luminescence starting delay and the concentration of the enzyme catalyst are in nonlinear negative correlation, that is, the delay time is obviously increased along with the reduction of the enzyme concentration. The nonlinear negative correlation is suitable for luminescent systems of different reinforcing agents. Benefited from the enzyme concentration dependent luminescence delay effect, the chemiluminescence liquid with the delay effect is used for improving the development quality of solid-phase membrane immunodetection for the first time, noise and background signals are suppressed, and the chemiluminescence liquid has wide application value in the aspect of solid-phase membrane immunodetection.

The invention discloses a chemiluminescence immunoassay method and kit for detecting progestational hormone of pandas, and belongs to the technical field of biological detection. The specific process comprises the following steps: by taking goat anti-mouse IgG as a coating antibody, enabling a progestational hormone standard substance or a to-be-detected sample and horse radish peroxidase labeled progestational hormone to compete with a limited amount of progestational hormone antibodies together; after incubation and washing, a chemiluminescent substrate is added, and quantitative analysis of the progestational hormone in the panda urine or excrement is realized by detecting the intensity of a luminescent signal according to a standard curve. The invention provides a novel chemiluminescence immunoassay method, the sensitivity of the chemiluminescence immunoassay method is far higher than that of a traditional ELISA method (the lowest detection line can reach 31.25 pg / mL), the operation is simple and convenient, the detection is rapid, and the urgent demand on rapid and accurate determination of the hormone level in panda pregnancy monitoring and reproduction management can be met.

The utility model relates to the technical field of medical instruments, in particular to a POCT (Point of Care Testing) chemiluminescence immunity analyzer which comprises a mounting box, an analyzer, an incubation assembly, a tray, a rotating frame, a limiting block, a guide frame and the like, an analyzer for detecting reagent cards is mounted on the mounting box, an incubation assembly is connected into the mounting box, a tray is connected to the incubation assembly, a rotating frame is rotatably connected to the incubation assembly, at least four limiting blocks for placing the reagent cards are connected to the rotating frame at intervals in the circumferential direction, and a guide frame is connected to the mounting box; the guide frame penetrates through the outer wall of the mounting box. The guide frame and the feeding frame are arranged, reagent cards are placed on the feeding frame, then the feeding frame is pushed into the limiting block along the guide frame, the reagent cards do not need to be held by hands to be inserted into the analyzer, cross infection between the hands and the reagent cards is avoided, the safety of the analyzer is improved, operation is simple, and operation is convenient. The workload of operators is reduced, and human errors can be avoided.

The application discloses a kind of filling assembly and control method, magnetic separation cleaning device.Adding component includes cup rotating member and injection needle, cup rotating member is used to grab reaction cup and drive it to rotate, and its central axis is provided with injection channel;Injection needle is coaxially arranged with cup rotating member and can be from injection channel into reaction cup.In control timing, cup rotating member first drives reaction cup to rotate, and after it is in rotating state, injection needle then adds cleaning fluid or substrate into reaction cup.The design will overlap the timing of filling and mixing steps, effectively reduce the filling splash and magnetic bead loss, improve the purification efficiency, and can complete efficient processing in the short residence time of magnetic separation carousel, especially suitable for high-throughput chemiluminescence immunoassay equipment, while ensuring detection accuracy significantly improve detection throughput.

The utility model relates to a reagent disc applied to an immunity analyzer. The reagent disc comprises a reagent disc, a fixed cover fixedly mounted at the top end of the reagent disc and a movable cover detachably mounted, a reagent pot is detachably mounted in the reagent disc, and the outer wall of the reagent pot abuts against the inner wall of the reagent disc; a reagent tray is rotatably installed in the reagent pot in a limited mode, a plurality of reagent boxes are sequentially rotatably installed on the reagent tray in the circumferential direction in a limited mode, pinions are detachably installed on the reagent boxes, fixed gears are detachably installed in the reagent pot, the fixed gears and the reagent tray are coaxially arranged, and the fixed gears are meshed with the pinions. A rotating mechanism is mounted on the reagent tray and is in transmission fit with the reagent tray; a refrigeration mechanism for cooling the interior of the reagent pot is mounted on the reagent pot; according to the chemiluminescence immunoassay kit, reagents for chemiluminescence immunoassay can be uniformly mixed and refrigerated, so that the accuracy of subsequent detection results is improved.

The invention relates to a theophylline immunodetection reagent as well as a preparation method and a detection method thereof, in particular to a theophylline chemiluminescence immunodetection reagent as well as a preparation method and a detection method thereof, and the theophylline chemiluminescence immunodetection reagent comprises an anti-theophylline specific antibody and an indicating reagent for detecting an anti-theophylline specific antibody-theophylline conjugate, the anti-theophylline specific antibody is obtained by immunizing animals with theophylline immunogen. The theophylline immunogen has the beneficial effects that the theophylline immunogen has strong specificity and high immunogenicity, and the prepared anti-theophylline specific antibody has strong specificity and high titer; the chemiluminescence immunoassay reagent containing the anti-theophylline specific antibody can conveniently, rapidly and accurately determine the theophylline content in a sample, can simultaneously determine a plurality of samples on a full-automatic chemiluminescence immunoassay instrument, realizes high-flux rapid determination of theophylline, and has the advantages of high accuracy, strong specificity, high sensitivity and high sensitivity. And the accuracy and the detection efficiency are greatly improved.

The invention relates to an anti-ovarian antibody chemiluminiscence kit and a preparation method thereof. The kit comprises amino magnetic beads coated with anti-ovarian antibody recombinant protein, anti-human immune globulin labeled by alkaline phosphatase, an anti-ovarian antibody calibrator and a chemiluminiscence substrate. The method has the beneficial effects that the 7-day acceleration stability of the AOAb coated amino magnetic bead is improved to 95% or above, the signal-to-noise ratio is high, the correlation coefficient is greater than 0.999, the test repeatability CV is less than 3.5%, the reagent correlation and the magnetic bead stability can be better improved, and the test result is ensured to be accurate. By optimizing the amino magnetic bead cross-linking agent, 60 clinical samples are tested and compared with the result of a comparison manufacturer, the total coincidence rate is as high as 98%, and false positive can be effectively reduced. Meanwhile, a detection system is formed by matching with a Guilin superior chemiluminescence immunoassay analyzer IA-260, the testing amount per hour reaches 130 tests, and compared with a traditional colloidal gold immunochromatography method and a traditional enzyme-linked immunosorbent assay method, the detection speed is greatly increased, and the result optimization accuracy is greatly improved.

1. Name of the design product: full-automatic chemiluminescence immunoassay analyzer. 2. Use of the design product: automatic detection equipment based on chemiluminescence immunoassay technology. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The application relates to the field of biotechnology, and in particular to a wheat allergen specific IgE detection kit and a detection method thereof. The kit comprises magnetic particles, enzyme-labeled anti-human IgE antibodies and biotin-labeled wheat allergen. The wheat allergen is obtained by treating a wheat protein extract with heat treatment or a pretreatment agent. The pretreatment agent comprises one or more of sodium azide, L-cysteine and L-cysteine hydrochloride. The wheat allergen is biotin-labeled after specific pretreatment. The chemiluminescence immunoassay method uses streptavidin to prepare magnetic microspheres and horseradish peroxidase-labeled anti-human IgE antibodies. The method is fully automatic, ensures the accuracy of the detection result, and improves the test flux, the specificity and the sensitivity of the detection result.

The utility model belongs to chemical detection technical field especially for a kind of mixing device for chemiluminescence immunoassay, including mechanical arm, the lateral slide of mechanical arm is equipped with transverse drive arm, the side of transverse drive arm is equipped with longitudinal drive arm. This kind of mixing device for chemiluminescence immunoassay, by sliding connecting block on integrated plate vertical sliding drive gripper assembly vertical movement, sample and reagent are added into reaction cup, after reaction cup is grabbed by gripper assembly, vibration motor starts, drives vibration mounting seat and reaction cup vibration, rubber seat mainly provides a flexible connection, when gripper assembly vibration, it can provide a larger vibration amplitude to reach the mixing effect of sample and reagent in reaction cup. Such design, by the integration of gripper assembly and vibration motor, complete mixing action in the scheduling process of reaction cup, can simplify work flow, simultaneously, independent mixing mechanism is saved, and cost is saved.

The invention relates to a nano antibody for specifically recognizing a tacrolimus-antibody compound and application of the nano antibody, and belongs to the technical field of biological detection. A variable region of the nano antibody has three complementary determining regions CDR1, CDR2 and CDR3, can specifically recognize a tacrolimus-antibody compound, and can be used for detecting drugs by a double-antibody sandwich method; the kit is widely suitable for various immunodetection technologies such as an enzyme-linked immunosorbent assay (ELISA), a chemiluminescence immunoassay (CLIA), an electrochemical luminescence immunoassay (ECLIA), a fluorescence immunoassay (FIA), an immunochromatography test strip method and the like. According to the method, the limitation of a traditional competition method is overcome, high-sensitivity, high-specificity and high-stability detection is realized, the method can be adapted to various detection platforms, and a portable pretreatment process without centrifugation can also be realized.

The utility model relates to a sample rack advancing and retreating device of a chemiluminescence immunity analyzer, and belongs to the technical field of sample loading and unloading, the sample rack advancing and retreating device comprises a bottom plate, an outer frame is fixed on the front surface of the bottom plate, a partition plate and a bearing seat are fixed on the front surface of the bottom plate, a pushing mechanism is arranged on the front surface of the bottom plate, a transmission mechanism is arranged on the outer frame, and a connecting assembly is fixed on the transmission mechanism. And a driving mechanism is arranged on the outer frame. According to the sample rack advancing and retreating device for the chemiluminescence immunoassay analyzer, feeding and retreating partition is carried out through the partition plate, a rack body with a plurality of test tubes can be conveyed and detected through the arrangement of the sliding seat, the connecting seat and the extending seat, so that the use efficiency of the device is improved, and through the arrangement of the transmission mechanism and the driving mechanism, the sample rack advancing and retreating device is convenient to operate. A detected sample can be conveniently conveyed to the bearing base, material returning work of the device is achieved, the use convenience of the whole device is improved, feeding and discharging of the whole device are convenient, a plurality of test tubes can be detected at the same time, and the use efficiency is high.

The utility model discloses a magnetic bead cleaning system for a chemiluminescence immunoassay analyzer, which comprises a waste liquid container, a first container, a second container and a cleaning module, and the cleaning module comprises a plurality of extraction and injection needle groups, a solid-liquid separation needle, a liquid injection and cleaning unit and a liquid suction unit, each suction and injection needle group comprises a first liquid injection needle, an anti-overflow needle, a second liquid injection needle, a first suction needle and a second suction needle which are arranged in sequence, and the first suction needle, the second suction needle and the anti-overflow needle are connected with the liquid suction unit. The extraction and injection needle set is composed of five needles, two-injection and three-suction can be carried out, and the magnetic bead cleaning effect is ensured; besides, magnetic bead cleaning liquid is crystalline cleaning liquid, suction needles in the suction and injection needle sets have the risk of blockage, once blockage occurs, the cleaning liquid overflows to generate crystals, an anti-overflow needle is additionally arranged in each suction and injection needle set, and when the liquid level in the reaction cup exceeds a certain height, the excessive liquid can be pumped away through the anti-overflow needles. And liquid in the reaction cup is prevented from overflowing.

The utility model relates to a chemiluminescence immunoassay device and a magnetic washing structure thereof, and effectively solves the problems that the accuracy of a detection result is influenced because the magnetic field intensity of a permanent magnet group cannot be adjusted because a compound is separated from a liquid phase by mostly adopting a magnetic field generated by the permanent magnet group in the conventional magnetic separation washing device. Comprising a liquid injection assembly arranged on a supporting frame, the liquid injection assembly comprises a liquid injection fixing plate coaxially fixed to the supporting frame, and a plurality of liquid injection needles are arranged on the liquid injection fixing plate; the magnetic bead cleaning device is ingenious in structure and convenient to use, compatibility of magnetic bead cleaning to different magnetic beads is achieved through the arrangement of the electromagnetic iron core, the magnetic attraction block, the coil and the like, the problem that cleaning is not thorough due to the fact that the magnetic bead attraction force is large when the magnetic field intensity is high is solved, meanwhile, the magnetic attraction block does not demagnetize due to time increase, the magnetic field intensity is weakened, and the magnetic bead cleaning efficiency is improved. Therefore, the accuracy of the detection result is greatly improved.

The invention relates to preparation of a cyclic adenosine monophosphate (cAMP) detection kit by using a chemiluminescence immunoassay technology. Cyclic adenosine monophosphate is an important second messenger for cell signal transduction and has wide physiological functions. Cyclic adenosine monophosphate is small in molecular weight and poor in immunogenicity, so that when a corresponding antibody is prepared, a proper derivative site needs to be selected firstly, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a cyclic adenosine monophosphate hapten, preparation of a cyclic adenosine monophosphate complete antigen and an anti-cyclic adenosine monophosphate antibody, a method for measuring the concentration of cyclic adenosine monophosphate, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of a cyclic adenylate derivative. The cyclic adenylic acid derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The invention relates to a preparation method of a cyclic guanylic acid (Guanosine 3 ', 5'-cyclic guanylic acid, cGMP) detection kit based on a chemiluminescence immunoassay technology, and particularly relates to a preparation method of a cyclic guanylic acid (Guanosine 3 ', 5'-cyclic guanylic acid, cGMP) detection kit based on a chemiluminescence immunoassay technology. Cyclic guanylate is small in molecular weight and poor in immunogenicity. When a corresponding antibody is prepared, a proper isomer and a derivative site need to be selected firstly, and a derivative hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a cyclic guanylic acid hapten, a cyclic guanylic acid complete antigen, preparation of an anti-cyclic guanylic acid antibody, a method for measuring the concentration of cyclic guanylic acid, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of a cyclic guanylate derivative. The cyclic guanylate derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The utility model relates to the technical field of medical equipment, in particular to a chemiluminescence immunoassay analyzer which comprises a first shell, a tray assembly, a bottom plate assembly and a photomultiplier assembly, an x-axis movement assembly and a y-axis movement assembly are arranged on the bottom plate assembly, the photomultiplier assembly is transversely installed on the x-axis movement assembly, and the y-axis movement assembly is transversely installed on the y-axis movement assembly. The tray assembly is installed on the y-axis movement assembly, the transmitting end of the photomultiplier assembly is provided with the testing head sleeve assembly, the first shell is arranged outside the second shell in a sleeving mode, and the front panel assembly and the rear panel assembly are connected to the two sides of the first shell through screws respectively. The test head sleeve assembly comprises a z-axis motor, a lead screw nut mechanism and a test head, the z-axis motor is connected to the photomultiplier assembly through a mounting bracket, the z-axis motor is connected with the test head through the lead screw nut mechanism, and a spring is arranged in the test head. According to the utility model, the problems of large occupied space and high integration difficulty in vertical arrangement of the existing photomultiplier are solved.

The application discloses a kind of full-automatic chemiluminescence immunoassay analyzer, it is related to immunoassay analyzer technical field, the analyzer includes main casing and front casing, the front casing is set in the side end of main casing, the inside of main casing is provided with reaction disc, the periphery of reaction disc is provided with conveying assembly, sampling assembly, gripper assembly, photometric assembly, mixing assembly and magnetic separation component, compared with the analyzer of current setting reagent processing unit and its supporting isolation component, high-precision sample reagent storage environment is constructed by isolation component, fundamentally solve the problem that traditional analyzer is easily polluted by outside and energy consumption is too high in non-working state, in addition, the linkage of driving assembly, isolation component and collaborative component in the application ensures that reagent processing unit has fault redundancy capability, so that equipment can still complete current detection process when local fault, effectively improve work efficiency.

The present application relates to a kind of mycophenolic acid detection reagent and its preparation and detection method, specifically a kind of mycophenolic acid chemiluminescence immunoassay reagent and its preparation and detection method, comprising: anti mycophenolic acid specific antibody, for detecting the indicating reagent of anti mycophenolic acid specific antibody-mycophenolic acid combination;The above anti mycophenolic acid specific antibody is obtained by mycophenolic acid immunogen immunization animal.The present application has the advantages that: the mycophenolic acid immunogen of the present application is specific, and immunogenicity is high, and the prepared anti mycophenolic acid specific antibody is specific, and titer is high;Chemiluminescence immunoassay reagent containing the above anti mycophenolic acid specific antibody can determine the mycophenolic acid content in sample conveniently, quickly and accurately, and multiple samples can be simultaneously determined on full-automatic chemiluminescence immunoassay analyzer, realize the high-throughput rapid determination of mycophenolic acid, and the accuracy is high, and the specificity is strong, and accuracy and detection efficiency have been greatly improved.

This invention discloses a shake-type fully automated chemiluminescence immunoassay analyzer, belonging to the field of immunoassay analyzer technology. It includes a main body and a display screen. The main body has a detection port at its end, with a detection chip placed on the surface of the port. A groove is formed on the surface of the main body, and a placement assembly is located inside the groove. The placement assembly is used to place multiple sets of solution bottles to be tested. A sealing plate is provided on the surface of the groove, and a clamping assembly is provided on the surface of the placement assembly. This invention controls the output of a servo motor to drive a main gear to rotate coaxially. When the main gear rotates, it drives a secondary gear to mesh and rotate. When the secondary gear rotates, it drives a locking post to rotate coaxially. When the locking post rotates, it drives a first connecting plate to rotate coaxially. At this time, a second connecting plate located above the first connecting plate rotates coaxially under the action of a buffer assembly, thereby achieving centrifugal rotation of the solution bottles placed inside the placement seat, ensuring uniform mixing of the internal solutions.

The invention relates to the technical field of biology, in particular to a magnetic microsphere, a preparation method and application thereof and a reagent containing the magnetic microsphere. The preparation method comprises the following steps: firstly, preparing carboxyl-containing polymer microspheres by a one-step method, then carrying out heat treatment under an alkaline condition, and then carrying out THF oscillation to finally prepare porous polystyrene carboxyl microspheres with uniform pore channels, meanwhile, avoiding using divinyl benzene as a cross-linking agent, reducing the luminous background of the finally obtained magnetic microspheres, and improving the luminous efficiency of the magnetic microspheres. The method can be better applied to the field of chemiluminescence immunoassay. Due to the existence of a surface porous structure and carboxyl, the stability and the functionalization degree of the magnetic material are improved, the problems of magnetic leakage and magnetic shell layer falling are avoided, the surface of the porous ball is smoother and more uniform, and a solid foundation is laid for the subsequent coating process and reduction of non-specific adsorption of the surface of the magnetic ball; the magnetic ball is prepared by using a grinding method, and has the advantages of safety, no toxicity, easiness in operation, no pollution and easiness in industrial popularization.

The utility model belongs to the technical field of detection instruments, and discloses a chemiluminescence immunoassay analyzer. The chemiluminescence immunity analyzer comprises a rack, and a sample adding mechanism, a reagent adding mechanism, an incubation mechanism, a magnetic separation cleaning mechanism, a reagent storage mechanism, a sample injection mechanism, a photoelectric detection mechanism, a uniform mixing mechanism, a reaction cup arrangement mechanism and a cup grabbing hand mechanism which are arranged on the rack. The rack of the chemiluminescence immunoassay analyzer is designed to be rectangular, all mechanisms are arranged on the rack in a planar one-layer integrated manner, the layout scheme is reasonable and efficient, and the integration is high.

The utility model provides a blocking device for a track of a biochemical and immune online system, which is arranged at the tail end of a track of a chemiluminescence immunoassay analyzer, is used for blocking and blocking track transportation of the chemiluminescence immunoassay analyzer and a biochemical analyzer, cannot protrude out of the track, and can ensure that the length of the biochemical and immune online system is stable. By arranging the driving unit, the gear, the rack, the guide assembly and the barrier plate, the driving unit can drive the gear to rotate, then the rack is driven to slide on the guide assembly, and lifting of the barrier plate is achieved; when the sample frame needs to be blocked, the blocking plate is driven to ascend to block the sample frame from being conveyed from the chemiluminescence immunoassay analyzer track to the biochemical analyzer track; when the sample frame needs to be released, the barrier plate is driven to descend, the sample frame is released, and cross-track rail-changing conveying of the sample frame can be achieved; accurate blocking or releasing can be achieved without reducing the speed of the sample frame, collision of the sample frame is avoided, and sample splashing can be avoided.

The utility model provides a light excitation chemiluminescence immunoassay reagent disc, which comprises a disc body and a reaction hole unit arranged on the disc body, and the reaction hole unit comprises a pre-reaction hole, a main reaction hole and a signal amplification hole which are sequentially connected in series to form a three-stage reaction channel; independent micro heating films are respectively integrated at the bottoms of the pre-reaction hole, the main reaction hole and the signal amplification hole; connecting runners are arranged between the pre-reaction hole and the main reaction hole as well as between the main reaction hole and the signal amplification hole, and fluid flow is controlled through centrifugal force or capillary force; a micro-column array or a micro-cavity array is arranged in the pre-reaction hole, and the surface of the micro-column array is modified with a biomolecule capture layer; a micro-nano composite structure is arranged in the main reaction hole, and enzyme or probe molecules are immobilized on the surface of the micro-nano composite structure. According to the utility model, the technical problems of insufficient reaction steps and low temperature control precision of the reagent disc in the prior art can be effectively solved.

The invention discloses an application of an improved isoluminol luminescent substrate in a MaglumiX8 chemiluminescence immunoassay system, and belongs to the technical field of in vitro diagnostic reagents. The luminescent substrate comprises an alkaline solution containing a metal leaf line complex and a derivative thereof, and a piperazine phenol derivative, and a peroxide solution containing urea and stannate. According to the invention, a metal leaf line substance is added into an alkaline solution, an active structure is stabilized through coordination modification, a reaction with hydrogen peroxide is promoted, a chemiluminescence signal is enhanced, and the luminous efficiency and the detection sensitivity are improved; by adding the piperazine phenol derivative, lasting glow of isoluminol is realized, signal stability is ensured, and result reliability is improved. In a hydrogen peroxide system, a urea / stannate stabilizer is added, so that the stability of a substrate is improved. Experimental verification shows that the substrate has high accuracy correlation with an original reagent, excellent precision and stable performance after being stored at room temperature for 24 months, and is suitable for the detection fields of hormones, tumor markers and the like.

The application provides a diluent of a peroxidase conjugate and application thereof, and the diluent comprises a basic buffer, a coagulant, a surfactant, an alkali metal chloride and an inert protein; the surfactant is selected from one or more of polyoxyethylene fatty alcohol ether-1, polyoxyethylene fatty alcohol ether-4 and polyoxyethylene fatty alcohol ether-5; the concentration of the coagulant is 0.02w / v% to 0.1w / v%; the concentration of the surfactant is 0.02w / v% to 0.5w / v%; the concentration of the alkali metal chloride is 0.5w / v% to 1w / v%; and the concentration of the inert protein is 0.1w / v% to 0.5w / v%. The diluent can reduce the background signal value of chemiluminescence immunoassay, and improve the signal-to-noise ratio of each detection index, so as to improve the sensitivity and accuracy of the chemiluminescence immunoassay technology.