Magnetic particle chemiluminescence immunoassay kit of free thyroxine

A technique of free thyroxine and chemiluminescence immunity, which is applied in the field of immunoassay medicine, can solve the problems that cannot be widely used in clinical diagnosis and scientific research work, and is limited in popularization and use, and achieves simple and reliable pretreatment process, convenient use and simple structure. Effect

Inactive Publication Date: 2010-02-03
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Detection of FT in prior art 4 The chemiluminescence immunoassay kit is a closed automatic chemiluminescence measurement system, which requires an expensive automatic chemiluminescence measurement instrument, which limits the promotion and use, and cannot be widely used in clinical diagnosis and scientific research.

Method used

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  • Magnetic particle chemiluminescence immunoassay kit of free thyroxine
  • Magnetic particle chemiluminescence immunoassay kit of free thyroxine
  • Magnetic particle chemiluminescence immunoassay kit of free thyroxine

Examples

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Embodiment 1

[0058] Embodiment 1 FT of the present invention 4 Preparation of Magnetic Particle Chemiluminescent Immunoassay Assay Kit I

[0059] 1), FT 4 Preparation of calibrator

[0060] A) Preparation of hormone-free human serum: put 60mL of normal human serum in equal parts into four volumetric flasks, then add 8.0g of activated carbon respectively, mix evenly with a vortex mixer by inversion, shake for 8h, centrifuge at 6000rpm for 20min, and The supernatant was filtered, and the preservative Proclin-300 with a volume percentage concentration of 0.5‰ was added to the filtrate, and it was frozen.

[0061] B) Add different amounts of FT to the hormone-free human serum obtained in step A) 4 The pure product was diluted into a calibration product, and after the concentration was determined by the radioimmunoassay method, the concentrations were divided into the following order: 0pmol / L, 1.59pmol / L, 3.45pmol / L, 15.6pmol / L, 37.5pmol / L, and 122pmol / L 6 bottles of FT 4 calibrator.

[0...

Embodiment 2

[0082] Embodiment 2 FT of the present invention 4 Preparation of Magnetic Particle Chemiluminescence Immunoassay Assay Kit II

[0083] 1), FT 4 The preparation of calibrator is the same as embodiment 1

[0084] 2), preparation of anti-FITC monoclonal antibody coated magnetic particle solution

[0085] Activate the magnetic particles with a particle size of 2.0 μm with glutaraldehyde, stir at room temperature, and mix for 2 hours, then strengthen the magnetic field with a strength of 2000 gauss, let it stand for 25 minutes, pour out the supernatant, and use 0.01mol of pH value 7.4 Wash 4 times with / L phosphate buffer solution, and use this solution to suspend at a concentration of 50 mg / mL; add 80 μg of anti-FITC monoclonal antibody to each ml of suspension, stir overnight at 4°C, and then apply a magnetic field with a magnetic field strength of 2000 Gaussian, let it stand for 15min, pour out the supernatant, and use 0.02mol / L phosphate buffer (pH is 7.2) containing mass pe...

Embodiment 3

[0104] Embodiment 3 FT of the present invention 4 Preparation of Magnetic Particle Chemiluminescence Immunoassay Assay Kit III

[0105] 1), FT 4 The preparation of calibrator is the same as embodiment 1

[0106] 2), preparation of anti-FITC monoclonal antibody coated magnetic particle solution

[0107] Activate the magnetic particles with a particle size of 3.0 μm with glutaraldehyde, stir at room temperature, and mix for 2 hours, then strengthen the magnetic field with a strength of 2000 gauss, let it stand for 20 minutes, pour out the supernatant, and use 0.01mol of pH value 7.4 Wash 5 times with / L phosphate buffer solution, and use this solution to suspend at a concentration of 75 mg / mL; add 60 μg of anti-FITC monoclonal antibody to each ml of suspension, stir overnight at 4°C, and then apply a magnetic field with a magnetic field strength of 2000 Gaussian, let it stand for 10min, pour out the supernatant, and use 0.02mol / L phosphate buffer solution (pH is 7.2) containi...

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Abstract

The invention discloses a magnetic particle chemiluminescence immunoassay kit of free thyroxine (FT4), belonging to the medical field of immunoassay. The kit comprises: 1) free thyroxine series calibrator; 2) magnetic particle solution enveloped by anti-fluorescein isothiocyanate monoclonal antibody; 3) free thyroxine antibody solution marked by fluorescein isothiocyanate; 4) free thyroxine markedby horse radish peroxidase; 5) chemiluminescence substrate liquid; and 6) concentrated washing liquid. The invention also discloses a preparation method of the kit. The kit in the invention has advantages of simpleness, convenience, rapidness, sensitivity, stability and the like.

Description

technical field [0001] The invention belongs to the field of immunoanalysis medicine, and in particular relates to a free thyroxine magnetic particle chemiluminescence immunoassay kit and a preparation method thereof. The kit combines immunomagnetic particle separation technology and chemiluminescence immunoassay technology, with wide detection range and high sensitivity. Background technique [0002] The thyroid is the largest endocrine gland in the human body, and the average physiological weight of an adult is about 20-25g. The thyroid contains many round or oval acini of varying sizes. The acinus is surrounded by a single layer of epithelial cells, and the cavity of the acinus is filled with colloid. Glial is the secretion of acinar epithelial cells, the main component is thyroglobulin. The acinar epithelial cells are the site of synthesis and release of thyroid hormones, and the acinar lumen colloid is the storage of hormones. Thyroid hormones are important hormones...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543G01N33/535G01N21/76
Inventor 林金明靳辉肖勤
Owner TSINGHUA UNIV
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