The invention belongs to the technical field of
Raman spectroscopy, and particularly discloses a preparation method and application of a hydrogel in-situ coated Au-Pt nano-
enzyme flexible SERS (Surface Enhanced
Raman Scattering) substrate, and the preparation method comprises the following steps: synthesis of concentrated gold nanoparticles: stirring and heating a HAuCl4
aqueous solution to boiling, quickly adding
sodium citrate, stirring and heating to obtain a concentrated gold
nanoparticle solution; heating and refluxing the
mixed solution for 10-60 minutes until the
mixed solution becomes
wine red; gradually cooling to
room temperature while stirring to obtain concentrated gold nanoparticles; and preparation of the hydrogel in-situ coated Au-Pt nano-
enzyme flexible SERS substrate: mixing the hydrogel and concentrated gold nanoparticles according to a volume ratio, then adding H2PtCl6 and
ascorbic acid, and carrying out a reaction to obtain the hydrogel in-situ coated Au-Pt nano-
enzyme flexible SERS substrate Au-Pt-Atar. According to the prepared Au-Pt-
Agar, due to the fact that the Au-Pt-
Agar effectively coats the nano particles, the influence of the external environment (
oxygen, acid, alkali and the like) on the nano particles is prevented, the particles are evenly dispersed in the hydrogel, and the detection uniformity and stability of an SERS substrate are improved under the condition that the sensitivity is guaranteed.