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58results about How to "Sampling method is simple" patented technology
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Current sampling circuit used for frequency converter and sampling method thereof
InactiveCN101515764ALow costSampling method is simpleDc-ac conversion without reversalElectrical resistance and conductanceFrequency changer
A current sampling circuit used for frequency converter comprises six switch pipes, and a sampling resistance is connected in serious at a lower bridge arm output end of at least two phases of direct current bus negative end or frequency converter of the current sampling circuit; a current sampling method of the current sampling circuit includes step of collecting information of current passing through the sampling resistance by the sampling resistance connected in series in the sampling circuit. The invention further provides two schemes, in one scheme, only a sampling resistance is connected in serious at a negative end of the direct current bus of the current sampling circuit, direct current bus current information is collected by the sampling resistance connected in serious at the negative end of the direct current bus, furthermore three-phase current information of a motor is reconstructed; in the other scheme, a sampling resistance is connected in serious at each output end of the three-phase lower bridge arm of the frequency converter, and three-phase load current information is obtained by directly sampling. The invention has simple sampling method, is easy to realize in engineering, and can be applied in any AC motor-driven system of three-phase balance current.
Owner:宁波德业变频技术有限公司
Conjoint analysis method for estimating DNA abundance of fishes based on environment DNA technology
ActiveCN105154564ASampling method is simpleProtect resourcesMicrobiological testing/measurementSequence analysisConjoint analysis
The invention relates to a conjoint analysis method for estimating the DNA abundance of fishes based on an environment DNA technology. The conjoint analysis method includes the steps that the total DNA abundance of the fishes is acquired through timed and quantified PCR; the species composition proportion of the fishes is acquired through 454 GS-FLX Titanium sequencing analysis; conjoint analysis of target species is performed through sequencing data and timed and quantified PCR data; a relative distribution diagram of mitochondrial DNA of the fishes in an environment is drawn through sufer 8.0 software. Based on the environment DNA identification technology, weather species exist and how many species exist can be analyzed just by collecting water samples without depending on fish species capturing, a sampling method is simple, and fish resources can be protected to the maximum degree; meanwhile, for some species which are rarely distributed and difficult to capture, the method is still more effective than traditional fishing investigation, the method can effectively solve the problem that effective species specificity primers are difficult to obtain when multiple closely related species exist in samples, and NDA abundance information of all species can be known just by conducting experiment once respectively through the two technologies.
Owner:SHANGHAI OCEAN UNIV
WSN (wireless sensor network) intra-network data fusion method based on kernel density estimation and non-parameter belief propagation
InactiveCN102056192AAchieve accurate fusion characterizationSampling method is simpleNetwork topologiesData switching networksNODALSpectral density estimation
The invention discloses a WSN (wireless sensor network) intra-network data fusion method based on kernel density estimation and non-parameter belief propagation, which comprises data acquisition and data fusion. The data acquisition is that monitoring unions which are respectively composed of no less than three sensor nodes for gathering the monitoring data are constructed in a monitoring region, each monitoring union is corresponding provided with a union header node for collecting the monitoring data, the sensor nodes in each monitoring union are respectively used for gathering the monitoring data of an object entering the monitoring region; and the data fusion is that the gathered monitoring data are subjected to KDE (kool desktop environment) processing by the sensor nodes in the monitoring unions respectively, the processed data are transmitted and collected to the union header nodes through NBP (name bind protocol) processing, the collected data are subjected to gauss mixing by the union header nodes, the data after gauss mixing are subjected to Gibbs sampling fusion, and the fused result is acted as a characteristic of the monitoring data. The accuracy of the monitoring data can be improved under a noisy or an uncertain environment, and the accurate fusion characterization of the monitoring data of the multi-node unions can be realized.
Owner:GUANGDONG UNIV OF PETROCHEMICAL TECH
Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage
InactiveCN102160504AGood effectExpand the scope of spaceWithdrawing sample devicesReceptacle cultivationSeedlingEcology
The invention discloses a rhizobox culture device applicable for sampling rhizosphere in a forest seedling stage. The device a seedling growing chamber and a rhizosphere sampling chamber, wherein water-draining holes are arranged on the bottoms of both the seedling growing chamber and the rhizosphere sampling chamber; the two chamber are movably connected through a connecting part and then communicated with each other; isolating meshes are covered on the vertical plane, in which the connecting part is positioned, and on the water-draining holes respectively; and in the rhizosphere sampling chamber, an isolating layer for sampling soil at the positions with different distances from the root is arranged near the vertical plane of the connecting part. The device is suitable for the forest seedling stage with relatively thick root system and can sample microzone soil at the positions with different distances from the root conveniently, effectively and precisely.
Owner:NANJING FORESTRY UNIV
Method and device for measuring anaerobic ammonia oxidation rate and contribution rate of anaerobic ammonia oxidation for removing nitrogen in water/sediment system in situ
ActiveCN103293233AThe results of the study were close toRepresentativeComponent separationNitrogenAmmonia
The invention discloses a method and a device for measuring the anaerobic ammonia oxidation rate and the contribution rate of anaerobic ammonia oxidation for removing nitrogen in a water / sediment system in situ. The device comprises a pipe (11), a rubber plug (10) and accessories, wherein both ends of the pipe (11) are open, the rubber plug (10) is matched with the pipe (11), and the accessories are distributed on the rubber plug (10) in a penetrating mode; the rubber plug (10) is completely sealed with the pipe (11); the accessories on the rubber plug include a pressure regulating device, a stirring device and a sampling device. According to the method and the device, a 15N isotopic tracing method is utilized to in-situ culture and collect overlying water of homogeneous sediments within a certain space range of the water / sediment system, thereby further measuring soluble 29N2 and 30N2 to calculate the anaerobic ammonia oxidation rate and the contribution rate of the anaerobic ammonia oxidation for removing the nitrogen within a certain area of the water / sediment system. The method and the device can satisfy environmental conditions of simulating in-situ temperature, illumination, states of nutrient elements and the like, and typical samples are collected to be used for measuring the anaerobic ammonia oxidation rate and the contribution rate of the anaerobic ammonia oxidation for removing the nitrogen.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Kit for simultaneously quantifying and detecting niacin, nicotinamide and pantothenic acid
InactiveCN105954453AGood precisionImprove accuracyComponent separationBiological testingBlood Collection TubeSmall sample
The invention provides a kit for quantitatively detecting niacin, nicotinamide and pantothenic acid in peripheral whole blood at the same time, which mainly consists of dried blood slices (Whatman protein saver 903), capillary blood collection tubes, nicotinic acid and nicotinamide and pantothenic acid vitamin standard substance and its isotope internal standard, trichloroacetic acid, and also includes dry blood spot preparation, dry blood spot pretreatment and detection steps. The nicotinic acid, nicotinamide, and pantothenic acid dry blood spot quantitative kit provided by the present invention combines dry blood spot sampling technology with high performance liquid chromatography-tandem mass spectrometry technology for the quantification of B vitamins, a minimally invasive, minimal blood collection at the same time Realize the evaluation of the nutritional status of multiple B vitamins, and realize the quantitative work of simultaneous detection of multiple vitamins under low sample volume conditions that cannot be accomplished by metabolic enzyme-dependent methods and immunoassay methods.
Owner:首都儿科研究所 +1
Liver cancer diagnosing reagent and kit using saliva as detection samples
PendingCN105132559ASampling method is simpleConvenient sampling methodMicrobiological testing/measurementDNA preparationSaliva sampleBiomarker (medicine)
The invention belongs to the technical field of biological medicine and relates to a detection reagent and kit for diagnosing liver cancer. The reagent is a long non-coding RNA (lncRNA) H19 detection reagent and used for detecting saliva samples. The kit using saliva as detection samples and used for diagnosing the liver cancer comprises protease and the lncRNA H19 detection reagent. The saliva detection reagent and kit has the advantages that the biomarker sensitive and specific in liver cancer diagnosing is found in the saliva for the first time, and great significance to early detection and treatment of the liver cancer, death rate lowering and China medical burden relieving is achieved.
Owner:THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
Switching power supply and control circuit thereof
ActiveCN103916020AReduce Harmonic DistortionSimple structureEfficient power electronics conversionDc-dc conversionPower flowTotal harmonic distortion
The invention discloses a switching power supply and a control circuit of the switching power supply. The control circuit of the switching power supply comprises a peak current detection end, a feedback end, a driving end, a first voltage comparator and a pulse width modulation unit. The control circuit of the switching power supply further comprises a control end and a reference voltage adjusting unit, wherein the control end is suitable for receiving control voltage, the reference voltage adjusting unit is suitable for generating a peak value comparison reference voltage according to control voltage, and when the value of the control voltage is equal to zero V, or the value is larger than a first voltage value, the value of the peak value comparison reference voltage is a second voltage value. When the value of the control voltage is larger than zero V and smaller than or equal to the first voltage value, the value of the peak value comparison reference voltage is equal to the sum of a third voltage value and the value of the control voltagea, and the sum of the third voltage value and the first voltage value is equal to the second voltage value. According to the switching power supply and the control circuit of the switching power supply, the total harmonic distortion of the switching power supply is reduced.
Owner:SHANGHAI LUMIXESS TECH
Environmental protection monitoring device
InactiveCN108226611ASimple voltage and current samplingSmall structure sizeCurrent/voltage measurementEnvironmental resistanceOpen structure
The invention discloses an environmental protection monitoring device, comprising a voltage sampling unit, a current sampling unit, a device base, a circuit functional unit, and power and signal cables, wherein the voltage sampling unit and the current sampling unit are connected to the device base; a cable under test is clamped in via an open structure; the circuit functional unit is mounted in the device base; the power and signal cables are connected inside; the voltage sampling unit is used for acquiring a voltage sample signal of the cable under test; the current sampling unit is used foracquiring a current sample signal of the cable under test; the circuit functional unit is used for processing the voltage sample signal and the current sample signal. The power and signal cables areused for providing access for power and transmitting signals. The voltage and current sampling modes of the environmental protection monitoring device can be simplified; the environmental protection monitoring device is convenient to install.
Owner:NANJING XINLIAN ELECTRONICS +1
Detection method and kit for simultaneously detecting 19 elements in human body
InactiveCN108226271AQuantitatively accurateEasy to storePreparing sample for investigationMaterial analysis by electric/magnetic meansManganeseCadmium Cation
The invention relates to a detection method and a kit for simultaneously detecting 19 elements in a human body. The 19 elements are zinc, strontium, selenium, lithium, aluminum, molybdenum, arsenic, iron, barium, copper, lead, mercury, nickel, antimony, manganese, cadmium, thallium, chromium and cobalt respectively. The detection method comprises the following steps of (1), preparing diluent whichis a 0.1wt% to 2wt% nitric acid aqueous solution containing 1.5mg / L to 2.5mg / L of golden standard and 0.05wt% to 0.2wt% of triton 100, preparing a standard solution which is prepared by adding standard products of the 19 elements into the diluent, and preparing an internal standard solution which is prepared by adding standard products of internal standard elements into the diluent; (2), collecting urine at any time point, wherein the urine is diluted through the diluent, so that a to-be-detected sample is obtained; (3), detecting by adopting inductively coupled plasma-mass spectrometry (ICP-MS). The detection method can be used for simultaneously detecting the 19 elements in the human body; a sampling method is simple; a sample is easily stored; the pretreatment is simple; the sensitivity is high; the specificity is high, and the detection flux is high.
Owner:GUANGZHOU BIOHOP TECH INC
Intelligent control circuit of agricultural greenhouse geothermal heating system
InactiveCN104635530ARealize automatic operationWith temperature control functionProgramme control in sequence/logic controllersTemperature controlAgricultural science
The invention relates to an intelligent control circuit of an agricultural greenhouse, in particular to an intelligent control circuit of an agricultural greenhouse geothermal heating system. The intelligent control circuit comprises a power supply circuit, a temperature control circuit, a water shortage auto-stop circuit, a control output circuit and an open-phase protection circuit. The upper limit temperature signal end of the temperature control circuit is connected to the second input end of an OR gate c through an isolating diode a; the lower limit temperature signal end of the temperature control circuit is connected to the input end of an OR gate a; the output end of the OR gate a is connected to the first input end of the OR gate c; the output end of the OR gate c is connected to the input end of an OR gate b; the output end of the OR gate b is connected to the second input end of the OR gate c through an isolating diode b; the output end of the OR gate c is connected to a base of a triode a through a drive resistor a; a collector of the triode a is connected to a first relay coil; a normally open contact of a first relay is connected between the control end a and the control end b. The intelligent control circuit allows the agricultural greenhouse geothermal heating system to automatically run, and the environment good for growth of fruits and vegetables is created for the agricultural greenhouse.
Owner:长沙聚湘堂智能装备有限公司
Open-phase protection circuit composed of voltage comparators
ActiveCN104485642ASimple structureLow costEmergency protective circuit arrangementsHemt circuitsControl theory
The invention discloses an open-phase protection circuit composed of voltage comparators, and relates to an open-phase protection circuit which includes a power supply circuit, a sampling circuit, a voltage comparator circuit and a control output circuit, wherein the power supply circuit includes a rectifier transformer, a single phase bridge type rectifier circuit, a filter capacitor a, a stabilizing integrated block and a filter capacitor b; the sampling circuit includes a phase line detecting element a, a phase line detecting element b, a phase line detecting element c, a rectifier diode a, a rectifier diode b, a rectifier diode c, a rectifier diode d, a rectifier diode e, a rectifier diode f, a voltage dividing resistor a and a voltage dividing resistor b; the voltage comparator circuit includes a voltage comparator a, a bias resistor a, a bypass resistor a, a voltage comparator b, a bias resistor b and a bypass resistor b; the control output circuit includes a driving resistor, a triode, a clamping diode, a relay, an interlocking control end a and an interlocking control end b. The open-phase protection circuit provides open-phase protection and low-voltage protection effectively for a three-phase motor, and is simple in structure.
Owner:ZHEJIANG HETAIXING CHEM FIBER CO LTD
Application of lnc-PCDH9-13:1 detection reagent to preparation of liver cancer diagnosis reagent/kit
InactiveCN107254521AConvenient sampling method for collecting salivaSampling method is simpleBioreactor/fermenter combinationsBiological substance pretreatmentsMortality rateTrue positive rate
The invention belongs to the field of biological medicine, and relates to a liver cancer diagnosis reagent / kit which comprises an lncRNA (long non-coding ribonucleic acid) lnc-PCDH9-13:1 detection reagent, wherein the reagent takes saliva as a sample in detection. A biomarker for diagnosing liver cancer sensitivity and specificity is found in the saliva for the first time, and the reagent / kit is of great significance for early diagnosis and treatment of liver cancer, reduction of death rate and relief of Chinese medical burden.
Owner:THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage
InactiveCN101435751AUnderstand the real state of water qualityReduce mistakesWithdrawing sample devicesEngineeringBottle
The invention provides a method and a device for collecting a water sample in a scallop culturing cage. The method is characterized by comprising: a sampling pipe is inserted in the cage through a hole of a base plate of the scallop cage, and is arranged in the center of the scallop cage; the other end of the sampling pipe is fixed on a buoyant raft of a culturing raft rack; sampling is started after adaption of between 3 and 5 days; one end of the sampling pipe is taken off from the buoyant raft, and is closely connected with a sampling pipe connection plug on a rubber plug of a sampling bottle mouth; a vacuumizing pipe of a manual vacuum pump is closely connected with a vacuumizing pipe connection plug on the rubber plug of the sampling bottle mouth; water in the sampling pipe is pumped and poured first, and then is vacuumized by the manual vacuum pump, so that water sucked in the sampling bottle can be taken to a laboratory for analyzing treatment after reaching a required amount. The method can collect on-site water sample without interrupting cultured creatures, thereby ensuring scientificity and effectiveness of marine ecologic environmental monitoring.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Sampling device and sampling method for pond-culture flatfish
The invention discloses a sampling device and a sampling method for a pond-culture flatfish, and belongs to the field of marine fish culture equipment. The sampling method comprises a funnel-shaped outer net cage, a large funnel net, a small funnel net, a large bamboo circle, a small bamboo circle, a stay bar, an edge line, a float line, a sinker line, a buoy, a buoy rope, a cross rope anchorage, a tail end anchorage, a tail end rope, a tail end anchorage line and a cross rope, wherein the sampling device is paved in a pond during sampling, and is taken in the next day, so that the requirements that regular sampling observation analysis is needed in the bastard halibut culture process and the sample n is greater than or equal to 30 are met; and the fish body is free of injury. The sampling device is low in price, simple in structure, easy to manufacture, and convenient to operate; the number of collected samples is ensured; the injuries to the fish body are reduced or avoided; the sample deviation is relatively small; and the condition of the pond-culture flatfish population can be really reflected.
Owner:DALIAN FUGU FOOD CO LTD
Motor counter electromotive force sampling system and method
ActiveCN107994817AReduce control complexityImprove Sampling AccuracyElectronic commutation motor controlEngineeringElectromotive force
The invention relates to a motor counter electromotive force sampling system and method. The system comprises a motor counter electromotive force sampling unit that is connected with a motor counter electromotive force sampling control unit; the motor counter electromotive force sampling control unit acquires working state data of a wire feed motor; the motor counter electromotive force sampling unit is controlled to stop sampling motor counter electromotive force upon power supply continuation and wire feed loop inductive load recovery; motor counter electromotive force is sampled to maximumextent upon motor counter electromotive force occurrence, sampling is not subject to wire feed loop impedance and loop inductance, and counter electromotive force sampling accuracy is improved accordingly. A hardware circuit is directly used herein to control motor counter electromotive force sampling; the sampling method is simple and effective, the circuit is simplified, and the cost is lowered;counter electromotive force sampling precision is guaranteed to maximum extent, and control precision is improved for a wire feed motor.
Owner:SHANGHAI HUGONG ELECTRIC WELDING MACHINE MFG
On-line particle image particle size analysis device
PendingCN106885763AHigh precisionSampling method is simpleWithdrawing sample devicesParticle size analysisComputational physicsAir compressor
The invention discloses an on-line particle image particle size analysis device, which comprises a sampling container, a sampling compressed air pipe, a sampling pipe, a material dispersion connector, an air compressor, a high-speed image pick-up device, a powder return pipe and an upper computer, wherein granular materials fall into the sampling container, are sucked into the sampling pipe, and are conveyed into the material dispersion connector through the sampling pipe; compressed air generated by the air compressor is introduced into the material dispersion connector; the materials in the material dispersion connector are blown away by the compressed air; the materials with uniform thickness fall into a high-speed image pickup region; a high-speed camera performs high-speed photo taking on the dispersion materials fell in the high-speed image pickup region; the particle graduation of the dispersed materials is recorded; the upper computer collects images shot by the high-speed camera; the shot images are analyzed through particle analysis software; the specimen particle size distribution condition is obtained. The on-line particle image particle size analysis device has the advantages that the concentration influence of taken materials is avoided; the measurement data precision is high; data such as morphological parameters can be obtained.
Owner:上海万澄环保科技有限公司
Rapid chick embryo egg sex identification method
InactiveCN106834460AThe identification steps are simple and feasibleImprove accuracyMicrobiological testing/measurementAutopsy resultMortality rate
The invention discloses a novel rapid chick embryo egg sex identification method which comprises the following steps: hatching an egg to 16, 17 or 18 days of age, aseptically obtaining allantoic fluid (10 microliters) from the tip of the embryo egg through a 1 ml of injector, sampling and sealing with paraffin wax for normal hatching; taking the obtained allantoic fluid as a template, designing a CDH-W specific primer for PCR amplification, detecting the amplified primer through gel electrophoresis and performing sex identification, wherein double bands (600 bp and 450 bp) are adopted for male amplification, and a single band (450 bp) is adopted for female amplification; after normal hatching, statistically calculating the hatching rate and the death rate, and performing biostatistical analysis with a control group; comparing a sex identification result with an autopsy result of a hatched chick, performing correlation analysis, and statistically calculating the accuracy rate of sex identification (if reaching 99 percent, the accuracy rate is deemed to be 100 percent of effective).
Owner:YANGZHOU UNIV
Method and system for predicting ultra-high-order harmonics generated by pulse width modulation
ActiveCN113131479AEasy to moveReduce power consumptionAc-dc conversionHarmonic reduction arrangementPower qualitySample Mode
The invention discloses a method and a system for predicting ultra-high-order harmonics generated by pulse width modulation. The method comprises the following steps of: acquiring a sampling mode of the pulse width modulation, a topological structure of pulse width modulation equipment and waveforms of modulated waves and carrier waves; deducing a dual Fourier transform formula in a modulation converter system by using a Fourier transform basic theory; calculating undetermined parameters in the deduced dual Fourier transform by using the waveforms of the modulated waves and the carrier waves, and calculating coefficients of sampling under different rules; and obtaining a mathematical model of an ultra-high harmonic generation result under a corresponding application scene and the sampling mode by using the calculated coefficients of the sampling under different rules, determining the amplitude of the ultra-high harmonics under the corresponding frequency according to the coefficients of the sampling under different rules, and processing the ultra-high harmonics with the amplitude higher than 0.05 pu to realize ultra-high harmonic prediction. According to the method and system, theoretical and method bases are provided for pulse width modulation mode design, ripple filter design, ultra-high harmonic influence evaluation and the like developed for reducing ultra-high harmonics, and the electric energy quality of a power grid system rich in a power electronic grid-connected converter can be improved.
Owner:STATE GRID CORP OF CHINA +1
Collection method and measurement method for alkali metals and compounds thereof in air in working place
ActiveCN103499654ASimple structureLarge adsorption capacityComponent separationWithdrawing sample devicesAbsorption capacityAlkali metal
The invention discloses a collection method for alkali metals and compounds thereof in air in a working place. The sampling can be finished by introducing the air in the working place into a sulfuric acid and methanesulfonic acid mixed aqueous solution which serves as an absorption medium. The collection method is simple, high in collection efficiency and high in absorption capacity. The invention also discloses a measurement method for the alkali metals and the compounds thereof in the air in the working place. The measurement method comprises the following steps of preparing a standard sequence of alkali metal ions; measuring a standard series, a sample and a blank reference solution through an ion chromatograph, drawing a standard curve, subtracting a response value measured by the sample into the standard curve to obtain the concentration of the alkali metal ions in the sample, and carrying out conversion to obtain the concentration of the alkali metals and the concentration of the compounds of the alkali metals in the air in the working place. According to the collection and measurement methods, various alkali metals and the compounds thereof can be simultaneously collected and measured through one-time sampling and under a chromatographic condition; the chromatographic measurement operation is simple; the stability is high; a result is reliable.
Owner:ZHEJIANG ACAD OF MEDICAL SCI
Farmland irrigation water sampling method
InactiveCN104977185AImprove adsorption capacityLow costWithdrawing sample devicesPreparing sample for investigationActivated carbonSorbent
The invention provides a farmland irrigation water sampling method including the procedures of activated carbon purification, activated carbon absorption bag preparation, sampler installation, sampling and target substance elution; activated carbon after purification is put into a small bag and sealed, an activated carbon absorption bag is prepared, the activated carbon absorption bag is mounted on a sampling rod to prepare a sampler, the sampler is placed in farmland or an irrigation water channel for sampling, a target substance in the activated carbon absorption bag taken from sampling is eluted, and a to-be-measured solution is obtained. The sampler produced with activated carbon as an adsorbent, has quite strong adsorption capacity on various heavy metals, has low cost, has the consistent adsorption capacity after being soaked in water for 30 days, and has the function of long-term accumulation adsorption acquisition of the target substance. The sampling method is simple and is convenient to operate, and provides a sampling method for the heavy metal total amount in water in a longer period for pollution monitoring and evaluation works of the farmland irrigation water.
Owner:农业部亚热带果品蔬菜质量监督检验测试中心
PCR (Polymerase Chain Reaction) primer, reagent or kit and identification method for identifying or assisting in identifying freshwater fish species
PendingCN113564262AAccurate identificationDo not cause interferenceMicrobiological testing/measurementDNA/RNA fragmentationNucleotideZoology
The invention provides a novel PCR (Polymerase Chain Reaction) primer (16s 200) for identifying or assisting in identifying freshwater fish species. A nucleotide sequence of the primer is SEQ ID NO.1 in a sequence table. The PCR primer is applied to eDNA metabolism coding of a fish mitochondrial DNA sequence. The PCR primer is adopted to perform PCR amplification on eDNA in a water body, based on a high-throughput sequencing method, the problem that an effective species specific primer is difficult to obtain under the condition that various closely-related species exist in the water body can be effectively solved, and based on an environmental DNA monitoring technology, an effective technical support is provided for taxonomy research and diversity protection of fishes. The method for identifying the freshwater fish species by using the PCR primer has the advantages of short consumed time, simplicity and convenience in operation, high specificity and the like.
Owner:SHANGHAI OCEAN UNIV +1
Method for quickly judging softening effect of water sample of water source well
ActiveCN110554160ASimple methodStrong targetingWithdrawing sample devicesTesting water hardnessWater softeningWater source
The invention belongs to the technical field of water softening, and particularly relates to a method for rapidly judging a softening effect of a water sample of a water source well. The method specifically comprises the following steps: measuring a theoretical value of a balance constant K; sampling a target water source; testing the mineralization degree and the calcium and magnesium ion concentration of the target water source water sample; determining a correction value of the balance constant K; calculating a residual hardness value C of the target water source; judging a water source well. The method has the advantages of quickness, simplicity, pertinence, high operability and high accuracy, the softened residual hardness value can be speculated only by testing the mineralization degree, calcium ions and magnesium ions of the water sample of the water source well, and softening experiments or industrial softening does not need to be carried out indoors or in a steam injection center. According to the method, the water source well is firstly judged and then dug, the problems existing in a traditional method that the water source well is firstly dug and then judged are solved,the water source well digging success rate is increased, and manpower and material resources are saved.
Owner:CHINA PETROLEUM & CHEM CORP +1
Multiple PCR primers for detecting lily viruses and application of multiple PCR primers in RNA extraction-free rapid detection method of lily viruses
PendingCN112458214ASimple extraction methodSampling method is simpleMicrobiological testing/measurementMicroorganism based processesRNA extractionSubcutaneous tissue
The invention discloses multiple PCR primers for detecting lily viruses. The lily viruses are cucumber mosaic virus (CMV), lily symptomless virus (LSV) and lily mottle virus (LMoV) respectively. Meanwhile, the invention further provides application of the multiple PCR primers in RNA extraction-free rapid detection method of lily viruses. The multiple PCR primers have the advantages that firstly, an RNA extraction method is simplified, a dissecting needle is adopted for picking trace subcutaneous tissues of lily and rapidly transferring the trace subcutaneous tissues to a reverse transcriptionreaction solution, a sampling method is simple, convenient and easy to operate, intermediate links capable of causing RNA degradation and pollution are omitted, an RNA extraction kit is not needed, the detection cost is reduced, and the working efficiency is improved; and secondly, lily virus CMV, LSV and LMoV primers are independently designed and developed, three pairs of specific primers with clear PCR product electrophoresis bands and good repeatability are screened out, amplified fragments are recycled for sequencing, the sequence alignment homology reaches 98% or above, it is proved thatgene fragments obtained through amplification of the primers designed and developed through the method are lily CMV, LSV and LMoV indeed, and it is guaranteed that a detection result is true and reliable.
Owner:甘肃省农业科学院生物技术研究所
A single cell sampling device and sampling method based on a porous metal enrichment probe
ActiveCN105713829BSimple preparation processLow costBiological material testing proceduresPlant tissueMicrometer
The invention discloses a unicellular sampling device and method based on a porous metal enrichment probe, and belongs to the technical field of unicellular sampling. The problem that existing unicellular sampling devices and methods based on capillary tube probes are high in cost, poor in repeatability and complicated in process is solved. The sampling device comprises a linear controller, the porous metal enrichment probe and a microscope, wherein the linear controller is composed of a base, a supporting rod, a clamping device, a micrometer head and a fixing base, the porous metal enrichment probe has a three-dimensional continuous porous structure, one end of the porous metal enrichment probe is a tip end, the other end of the porous metal enrichment probe is fixed to the fixing base, the porous metal enrichment probe moves in the axis direction of the micrometer head along with a measuring rod of the micrometer head, and the microscope is used for observing cells in tissue to be sampled. The unicellular sampling device is easy and convenient to operate, good in repeatability, low in cost, high in efficiency and capable of achieving unicellular sampling of animal and plant tissue, then, rapid analysis of unicellular metabolins is achieved, and differences between the metabolins among different cells are found.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Narcotic saliva detection
InactiveCN101526532ASampling method is simpleNot susceptible to adulterationMaterial analysisMonoclonal antibodyTrue positive rate
The invention relates to narcotic saliva detection belonging to the field of biological medicines, biological separation, biological devices, reagents and relative detection reagents. A colloidal gold immunity tomography technology is very developed as a development platform technology for the product, but the development key of the product lies in the high sensitivity and the high specificity of a monoclonal antibody and the optimization of the product manufacture technological process. In order to evade the technological problems, the monoclonal antibody used for product development can be firstly separated so that monoclonal antibody stubs with the optimal sensitivity and the optimal specificity can be selected for protein labeling and can meet the special requirements for the saliva detection.
Owner:陈桂勇
Periphytic algae sampling device
PendingCN109085021ASampling method is simpleThe collection steps are simpleWithdrawing sample devicesAlgaeTorsion spring
The invention provides a periphytic algae sampling device. The periphytic algae sampling device comprises a handle, a hollow connecting rod, a cable, a cable driving component and a sampling barrel, the periphytic algae sampling device is characterized by: the cable driving component is arranged on the handle, the handle is connected with the upper end of the hollow connecting rod, and the lower end of the hollow connecting rod is connected with the sampling cylinder; a separator is arranged in the sampling barrel, a through hole is arranged on the separator, a movable plug is arranged on thetop surface of the separator, and the movable plug can cover the through hole; a first rotating shaft is arranged on the bottom surface of the separator, a rotating disc is arranged on the first rotating shaft, and a brush body is arranged on the bottom surface of the rotating disc; a torsion spring is arranged between the rotating disc and the separator, and a cable arrange groove is arranged onthe side surface of the rotating disc; the cable passes through the hollow connecting rod, the upper end of the cable is connected with the cable driving component, and the lower end of the cable is fixedly connected with the cable arrange groove on the side surface of the rotating disc. According to the periphytic algae sampling device, periphytic algae and in situ water can be collected at the same time, the periphytic algae sampling device is convenient to use and can greatly improve the sampling efficiency.
Owner:NANCHANG INST OF TECH
Device for collecting trace dust in gas and method for detecting particle diameter and content of particles
PendingCN107907388ASampling method is simpleFlexible sampling methodWithdrawing sample devicesParticle size analysisAnalytical chemistryPhysics
The invention discloses a device for collecting trace dust in gas and a method for detecting the particle diameter and the content of particles. The device comprises a dust filter, a three-way valve,a gas volume flow rate meter and an injector, wherein the dust filter is connected with the gas volume flow meter and the injector through the three-way valve. The invention also discloses the methodfor collecting and detecting the particle diameter and the content of particles of the trace dust in the sample gas. The device and the method can be applicable to the conditions that the sample gas quantity is less than 100mL and the dust content is less than 5mg / L, and can also be applicable to the conditions that the gas quantity is greater than or equal to 100mL and the dust content is greaterthan or equal to 5mg / L; the problem that the detection of the trace dust in the gas is limited by the sample quantity and the dust content is solved.
Owner:SOUTH CHINA UNIV OF TECH
An intelligent control circuit of a geothermal heating system for agricultural greenhouses
InactiveCN104635530BRealize automatic operationWith temperature control functionProgramme control in sequence/logic controllersElectrical resistance and conductanceLower limit
Owner:长沙聚湘堂智能装备有限公司
Wheat weighing and accurate sampling bin
PendingCN114208507AFor long-term storageSampling method is simpleAgriculture tools and machinesWithdrawing sample devicesAgricultural scienceAgricultural engineering
The invention belongs to the technical field of grain storage bins, and particularly relates to a wheat weighing accurate sampling bin which comprises a storage barrel, a cover body is arranged above the storage barrel, through holes are formed in the cover body and the storage barrel in an up-down corresponding mode, sampling outer pipes are fixedly installed in the through holes, first sampling openings are formed in the pipe walls of the sampling outer pipes, and second sampling openings are formed in the pipe walls of the sampling outer pipes. The bottom of the sampling outer pipe is provided with an electromagnetic valve for controlled discharging, the sampling inner pipe is coaxially arranged in the sampling outer pipe, the pipe wall of the sampling inner pipe is provided with a sampling port II matched with the sampling port I, the cover body is provided with a driving system for driving the sampling inner pipe, and the diameter of the outer wall of the sampling inner pipe is equal to that of the inner wall of the sampling outer pipe. The grain sampling device has the characteristics of accurate sampling and no influence on grain storage quality.
Owner:ZHENGZHOU LONGWEI ELECTRONICS TECH CO LTD
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