57 results about "Saliva sample" patented technology

The application discloses a method for determining 15 new psychoactive substances in saliva by solid phase supported liquid-liquid extraction (SLE) combined with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS), which specifically uses a mixed solution of methyl tert-butyl ether and n-hexane as an elution solvent, adopts solid phase supported liquid-liquid extraction to pretreat saliva samples, and then adopts UPLC-MS / MS to detect the collected eluent, so as to realize qualitative and quantitative determination of the new psychoactive substances in saliva. The method has the advantages of rapidness, accuracy, high sensitivity and simple operation, the detection limit of the method is 0.2-0.5 ng / mL, can meet the domestic and foreign limit requirements, and provides technical support for monitoring and investigation of the new psychoactive substances in saliva.

The application relates to the field of biotechnology and health informatics, and particularly discloses a microorganism marker combination for detecting obstructive sleep apnea of children and application thereof. The microorganism marker combination comprises the following nine microorganism genera: g__Streptococcus, g__Solobacterium, g__Schaalia, g__Capnocytophaga, g__Stomatobaculum, g__Mogibacterium, g__Clostridia_UCG-014, g__Selenomonas and g__Pseudoleptotrichia. By detecting the relative abundance of the above microorganism genera in a saliva sample of a child, a classification model is constructed by using a random forest algorithm, non-invasive and objective screening of OSA of the child is realized, and the application has high accuracy and clinical application value.

The present invention relates to a biosensor that enables the detection of biomarkers related to bone metabolism through saliva samples. This biosensor, which uses a saliva sampling method and offers an economical and user-friendly solution by adopting a non-invasive approach, can detect biomarkers related to bone formation and resorption such as Procollagen Type I N-terminal Propeptide (PINP) and β-C- terminal Telopeptide (β-CTX) in saliva with high sensitivity. The present invention increases patient comfort while also providing rapid analysis, making the diagnosis and treatment processes of diseases related to bone metabolism more efficient.

The invention relates to the field of disease prevention and control, in particular to an auxiliary monitoring system for epidemic disease protection. The invention provides the auxiliary monitoring system for epidemic disease protection, which can automatically collect a saliva sample of a detector, save manpower and prevent medical personnel from being infected with epidemic diseases. An auxiliary monitoring system for epidemic disease protection comprises a machine body, a small lead screw, a top cover and the like, the small lead screw is rotationally connected between the front portion and the rear portion of the machine body, and the top cover is clamped to the upper side of the machine body. According to the spittle collecting device, the spiral moving seat moves forwards to drive the safety disc to move forwards, meanwhile, the mouth of a detector is opened, a collecting cotton swab on an elbow moves to the throat of the detector, and the effects that the spittle sample of the detector can be automatically collected, manpower is saved, and medical workers are prevented from being infected with epidemic diseases are achieved.

This invention discloses a method for calculating stress age based on saliva extraction, belonging to the field of data processing technology. It includes collecting saliva samples and personal data from individuals, including individual characteristics, physiological data, and behavioral data; obtaining telomere detection data from the saliva samples; analyzing the telomere detection data, physiological data, and behavioral data to obtain telomere-related data; and generating a test report based on the telomere-related data. This method achieves the scientific quantification of emotional and psychological stress, and compared to traditional scales, the test results are more scientific and accurate.

A method for diagnosing prostate cancer comprising: providing a saliva sample from a subject, and measuring the expression of C4B (complement component 4) in the sample. Also claimed are separate meth

The invention discloses a non-invasive prediction method for acne risk of puberty women and a kit, and belongs to the technical field of biological detection. According to the present invention, the non-invasive advantage of the saliva sample is adopted, and the cycle specificity of saliva crystallization and the pathological correlation of the sex hormone ratio are combined to achieve the early accurate early warning of the acne risk, such that the problems of strong invasion and difficult promotion in the teenager group of the traditional serum detection are solved, and the characteristics of convenient operation, low cost and repeatable monitoring are provided; and a new technical means is provided for prevention and early intervention of acne of puberty women.

The invention provides a method for detecting the total antioxidant capacity of saliva based on iron-based MOF nano-enzyme, and belongs to the field of biosensing. The method comprises the following steps: obtaining an MIL-53Fe nano enzyme; the preparation method comprises the following steps: mixing a diluent of MIL-53Fe nano-enzyme, a sodium acetate buffer solution, 3, 3 ', 5, 5'-tetramethyl benzidine and hydrogen peroxide for reaction, adsorbing a reaction solution by using filter paper, drying and curing by using a chitosan solution to obtain the visual microfluidic detection paper-based chip. Establishing a standard curve of the chromatic value and the ascorbic acid concentration; dropwise adding the treated saliva sample onto the paper-based chip, standing for reaction to obtain a chromatic value, and calculating according to the standard curve to obtain the total antioxidant capacity value of the saliva. Complex matrix interference is broken through, the detection sensitivity and portability are improved, and therefore clinical application of saliva TAC detection in noninvasive health monitoring is promoted.

The invention relates to the technical field of biological detection, in particular to a saliva RF-IgM rapid detection kit and application thereof, and the saliva RF-IgM rapid detection kit comprises a saliva pretreatment module, a signal amplification module and a result interpretation module. The invention provides an RF-IgM rapid detection method based on a saliva sample and application thereof, and solves the problems of invasiveness and time consumption of traditional serological detection. Technical difficulties in saliva detection, including the problems of insufficient sensitivity and high false negative rate caused by large IgM molecular weight and saliva mucoprotein interference, are solved. Non-invasive, rapid and convenient detection of rheumatoid factors is achieved, patient compliance is improved, and the kit is suitable for outpatient real-time detection (POCT), primary medical treatment and large-scale population early screening.

The invention discloses an ergothioneine trimethylamine lyase mutant and application of the ergothioneine trimethylamine lyase mutant in detection of histidine betaine in saliva. L-histidine betaine is catalyzed by ergothioneine trimethylamine lyase to be hydrolyzed into urocanic acid, the light absorption value of the urocanic acid at 290 nm is detected by a colorimetric method, and the content of L-histidine betaine in saliva can be determined. The method is high in sensitivity, and detection of 12.5-100 mg / L histidine betaine in a saliva sample can be completed within 1.5 hours. The method is simple and convenient to operate and can be used for quickly judging the metabolic state of human ergothioneine.

The utility model provides a saliva sampling detection device which comprises a detection head, a drainage component, a locking part and a saliva storage component, a threaded head is installed on one of the detection head and the drainage component, and a threaded hole is formed in the other one of the detection head and the drainage component; the locking part comprises a limiting piece and a buckling piece, the limiting piece is installed on one of the detection head and the drainage component, and the buckling piece is installed on the other one of the detection head and the drainage component; the fastener comprises an intercepting surface, and when the limiting part makes contact with the intercepting surface, the detection head rotates in one direction relative to the drainage component. The detection head rotates forwards relative to the drainage component and is gradually screwed and connected, the limiting piece rotates relative to the drainage component along with the detection head, at the moment, the limiting piece does not make contact with the interception face of the fastener, and if the detection head rotates backwards relative to the drainage component, when the detection head and the limiting piece rotate by at most 360 degrees relative to the drainage component, the limiting piece makes contact with the interception face, and the detection head is stopped. The interception face prevents the limiting piece from rotating relative to the drainage component, and therefore separation of the detection head and the drainage component is limited.

The utility model discloses a portable saliva test box, and belongs to the field of saliva sample collection, the portable saliva test box comprises a test box, the top end of the test box is provided with a sealing cover, the bottom surface of the sealing cover is provided with a sampling rod, the bottom end of the sampling rod is provided with a sampling head, one side of the top surface of the test box is provided with a detection port, and the inner side surface of the test box is provided with a through port; and the through opening is communicated with the detection opening. The method has the effect of improving the accuracy of the detection result of the saliva sample.

The application discloses a method for screening metabolic biomarkers based on saliva metabolic fingerprint and application, and relates to the technical field of biological sample metabolic analysis. The method comprises the following steps: step 1, inorganic nanoparticles are used to enhance laser desorption ionization time-of-flight mass spectrometry technology, saliva samples of Parkinson's disease patients and healthy volunteers are subjected to metabolic detection, and saliva metabolic fingerprints are obtained; step 2, the saliva metabolic fingerprints obtained in step 1 are subjected to a machine learning algorithm, saliva samples are subjected to a variety of model training, a variety of models are evaluated through a cross-validation method, and a final prediction model is screened according to a verification result; step 3, saliva samples of Parkinson's disease patients and healthy volunteers are predicted according to the prediction model of step 2, a prediction result is obtained, and metabolic biomarkers in the saliva samples are screened according to the prediction result. The application has high screening efficiency, the result is accurate and reliable, and the screened metabolic biomarkers can be used for prediction of Parkinson's disease.

The application discloses a nucleic acid absolute quantification method based on nucleic acid constant temperature amplification, which comprises the following specific steps: step one, sample pretreatment, blood samples and saliva samples are pretreated respectively; step two, reaction system configuration and assembly; step three, digital micro-reaction unit preparation and amplification; and step four, multiple signal analysis and absolute quantification. In the application, multiple primer design and a double-probe system are adopted, so that the mutation detection rate is improved, and the detection limit can be reduced to 0.1% variation through Cas12a-sgRNA auxiliary verification. The double-emulsification technology of the micro-fluidic chip is adopted, so that the droplet diameter variation coefficient is controlled within 5%, and the clogged micropore rate is reduced by real-time analysis of the micropore array image through the ImageJ software, automatic marking and exclusion of abnormal units.

Provided herein are fluid sample collection devices, particularly, saliva sample collection devices, and methods of using them. In certain embodiments, the fluid sample collection device comprises: a first portion and a second portion, the two portions removably joined together, wherein each of the first portion and the second portion comprises a casing configured to removably accommodate an adsorbent sample collection pad. Each adsorbent sample collection pad comprises a sample collection element and a longitudinal element, which fits in a longitudinal groove of the casing. The casings removably connect with each other via connection elements to form the fluid sample collection device. In some embodiments, the adsorbent sample collection pad comprises an indicator dye, which provides a signal when contacted with a sample. In some cases, the first and / or the second portion comprise a holding element for a user. Methods of collecting saliva samples using the collection devices are also described.

An object of the present invention is to provide a reagent capable of reducing a non-specific reaction in the detection of a substance to be measured in a saliva sample using Substance 1 that is labeled with a peroxidase and that binds to the substance to be measured, a kit including the reagent, and a method for measuring a substance to be measured using the reagent. According to the present invention, there are provided a reagent containing a glycoprotein, in which, in detection of a substance to be measured in a saliva sample using Substance 1 that is labeled with peroxidase and binds to the substance to be measured, the reagent is a reagent for being brought into contact with the saliva sample prior to contact between the Substance 1 and the substance to be measured; a kit containing the reagent; and a method for measuring of a substance to be measured using the reagent.

The invention relates to a method for identifying an emotion or a group of emotions felt by a subject after olfactory stimulation, from saliva samples of said subject implementing salivary biomarkers signature. In a second aspect, the invention also relates to a method for evaluating the capacity of an olfactory stimulus to provoke in a subject a desired emotion or group of emotions. The invention finally relates to the use of kit in the context of the present invention.

The utility model relates to an anti-pollution saliva collection detector, and relates to the field of saliva detectors. The device comprises a detection piece and a sampling piece, the detection piece comprises a shell, the bottom surface of the shell is open, a bottom plate is assembled at the open end of the shell, a mounting groove is formed in the top surface of the bottom plate, a detection card is horizontally placed in the mounting groove, a funnel-shaped sample adding hole is formed in one side of the top surface of the shell in a penetrating manner, and the bottom surface of the shell is provided with an opening; a display hole is formed in the other side of the top surface of the shell in a penetrating manner, and the display hole corresponds to the detection card display area. The capacity of the sample adding hole is large, enough saliva samples to be detected can be collected, meanwhile, the situation that the saliva samples overflow during detection is avoided, and the detection result is affected by sample pollution possibly caused by sample overflow which pollutes test paper in other areas or makes contact with a detector shell; the hidden danger of sample overflow is solved by an enlarged containing area of a liquid collecting area of the sample adding hole and a funnel-shaped hole.

Disclosed is an in vitro method for assessing whether a human subject has periodontitis. The method comprises detecting, in a sample of saliva from said subject, the concentrations of the proteins Hepatocyte Growth Factor (HGF) and Matrix Metalloproteinase 8 (MMP-8). Based on the concentrations determined, and adding age, and possibly other demographic markers such as sex and / or BMI, a testing value reflecting the joint concentrations is determined for said proteins, in combination with one or more demographic markers. The testing value is compared with a threshold value. The threshold reflects in the same manner the joint concentrations and the age, and possibly other demographic markers, as associated with periodontitis and may be seen as an upper limit of testing values as seen in a population of subjects without periodontitis. Thereby a testing value at or above the threshold value is indicative for periodontitis in said subject.

An in vitro method for assessing or predicting the response of human patients to treatment of periodontal disease is disclosed. This method is based on insights into identifying biomarker proteins. Therefore, the concentration of a specific combination of proteins is measured in a saliva sample from the patient. One such combination is at least one of interleukin-1-β (IL-1β) and matrix metalloproteinase-8 (MMP-8), and α-1-acid glycoprotein (A1AGP). Based on the measured concentrations, at least one value reflecting the combined concentration of these proteins is determined. This at least one value can indicate the likelihood that the human patient has been or will be successfully treated for periodontitis. This at least one value can be compared to at least one threshold, which in the same manner reflects the combined concentration associated with successful treatment of periodontitis. This comparison allows assessment of whether the test value is an indicator of the patient's periodontal treatment status.

The invention belongs to the technical field of alpha fetoprotein visual quantitative analysis, and particularly relates to a visual quantitative analysis method for alpha fetoprotein in saliva, a double-recognition multiple-quenching molecular imprinting and electrochemical luminescence microscope is constructed, and MI-ECLM takes self-developed SECLM as a signal output device and comprises a molecular imprinting polymer and a double-mechanism quencher; preparing a saliva sample and performing pretreatment; the pretreated saliva sample is placed on an MI-ECLM sensor, and an optical image is obtained through electrochemical luminescence imaging; and analyzing the optical image to realize visual quantitative detection of alpha fetoprotein in saliva.

The invention discloses a colloidal gold immunochromatography test strip for saliva sample detection and a preparation method thereof.The test strip comprises a supporting layer, and a sample pad, a combination pad, an NC membrane and a water absorption layer are sequentially arranged on the supporting layer from the sample introduction end; at least partial areas of the sample pad and the combination pad are obtained by drying after being pretreated by saliva sample treatment liquid, and the saliva sample treatment liquid comprises the following components: a zwitterionic anti-adsorption buffer system, an antibody conformation relaxation agent, a chelating agent type colloid redispersant, hydrophilic small molecular protein and a cyclodextrin type hydrophobic shielding agent. According to the method, systematic improvement is performed from the biochemical performance of the sample and the state of the antigen target, so that the defects that the target is covered by the colloidal environment of saliva and the target is lost due to aggregation of the target antigen are effectively overcome, and the detection sensitivity is greatly improved.

Provided is a method of diagnosing and / or monitoring mild traumatic brain injury (mTBI) or concussion in a subject. The method comprises determining a level of at least one miRNA in a saliva sample from the subject. Also provided is a sensor element, a detection system, composition and a kit for diagnosing and / or monitoring TBI, and a method of determining an appropriate treatment for a subject with suspected mTBI or concussion.

The invention discloses a primer probe set for noninvasive detection of individualized medication gene loci, a kit and application, and belongs to the field of biotechnology and molecular diagnosis. The sequences of the primer probe group are as shown in SEQ ID NO. 1 to SEQ ID NO. 51. The primer probe group is prepared into the kit, the kit comprises 7 tubes of independent premixed PCR reaction liquid, and high-throughput typing of 17 SNP loci of 12 drug genes is realized through a single-channel or multi-channel fluorescent PCR and melting curve analysis technology and a same PCR reaction program. An oral swab or a saliva sample is adopted and can be directly used as a template for gene detection after being treated by a nucleic acid lysis solution. Besides, the kit has the advantages of simplicity and convenience in operation, high sensitivity, low cost and the like, is suitable for detecting drug metabolism gene related types on an assembly line platform, and can be used for clinical early-stage rapid screening and accurate intervention.

This invention provides a gene biomarker for the auxiliary diagnosis of lung cancer and its application. It screens for highly expressed gene biomarkers associated with lung cancer in saliva samples. The gene biomarker is composed of at least one of NANP, APOA5, ATG9A, GAL3ST1, BICC1, GPR143, LHX5, and EPS8L3. It can be used in lung cancer auxiliary diagnostic kits. The gene biomarker provided by this invention is derived from saliva and can be detected using a non-invasive method. The detection is safe and non-invasive, providing a basis for rapid screening, diagnosis, and precision treatment of lung cancer.

1. Name of the product in this design: Saliva Detector. 2. Purpose of this design: For testing saliva samples. 3. The key design feature of this product is its shape. 4. Images or photos that best illustrate the design key points: Use state diagrams. 5. The rear view is the same as the front view, so the rear view is omitted.

The present disclosure provides a test strip (100) for measuring chloride ion concentration in a sample The test strip (100) comprises a substrate (102) with a sample port (104) at one end and a sample elution area (106) adjacent to the sample port. The sample elution area (106) comprises a complexing agent, where a concentration of the complexing agent is pre-determined and is variable along the direction of sample elution in the sample elution area (106). The chloride ion concentration in the sample is measured from a detectable visual change in the sample elution area (106) resulting from a reaction of the complexing agent with chloride ions in the sample. A method for measuring chloride ion concentration in a sample using the test strip is provided. A method of preparing the test strip (100) is also disclosed. The test strip (100) finds applications in screening, monitoring and / or diagnosing cystic fibrosis from a salivary sample.

Disclosed is an in vitro method for assessing whether a human patient suffering from periodontitis has mild periodontitis or advanced periodontitis. The method is based on the insight to determine a selection of two biomarker proteins. Accordingly, in a sample of saliva a patient suffering from periodontitis, the concentrations are measured of the proteins Pyruvate Kinase (PK) and at least one of Matrix metalloproteinase-9 (MMP9), S100 calcium-binding protein A8 (S100A8), and Hemoglobin subunit beta (Hb-beta); or of the proteins Matrix metalloproteinase-9 (MMP9) and at least one of S 100 calcium-binding protein A8 (S100A8) and S100 calcium-binding protein A9 (S100A9). Based on the concentrations as measured, a value is determined reflecting the joint concentrations for said proteins. This value is compared with a threshold value reflecting in the same manner the joint concentrations associated with advanced periodontitis. The comparison allows assessing whether the testing value is indicative of the presence of advanced periodontitis or of mild periodontitis in said patient. Thereby, typically, a testing value reflecting a joint concentration below the joint concentration reflected by the threshold value is indicative for mild periodontitis in said patient, and a testing value reflecting a joint concentration at or above the joint concentration reflected by the threshold value, is indicative for advanced periodontitis in said patient.

This invention discloses a fluid sample sampling head and its application. The fluid sample sampling head has a porous fiber structure, and the hardening of its circumferential wall is less than that of its two ends along its axial direction. Under external pressure, the fluid sample sampling head undergoes linear expansion and contraction deformation, and its circumferential wall is used to collect bodily fluids. By utilizing its circumferential wall to collect bodily fluids, this fluid sample sampling head increases the surface area in contact with bodily fluids such as saliva, thereby better adsorbing saliva and increasing the amount of saliva adsorbed. Furthermore, the fact that the circumferential wall of the fluid sample sampling head has less hardening than its axial wall allows it to undergo linear expansion and contraction deformation under external pressure. Therefore, when bodily fluid is squeezed out of the fluid sample sampling head, the linear expansion and contraction deformation under external force better extrudes the absorbed saliva sample, increasing the amount of saliva collected.

The invention provides an individual biological age deduction method and system based on a saliva sample, and relates to the technical field of biological age deduction, and the method comprises the steps: obtaining a plurality of age-related methylation chip data samples; performing data processing on the methylated chip data sample to obtain a plurality of target CpG sites; carrying out site screening on each target CpG site through a regression model to obtain a target site set, and determining a first prediction age according to the target site set; according to a second prediction age and a third prediction age which are output through the SVM model and the ANN model, accuracy evaluation is carried out on the second Elastic Net regression model, the SVM model and the ANN model, and an optimal prediction model is determined; and outputting the predicted age output by the optimal prediction model as an individual biological age inference result. According to the method, higher-precision, higher-robustness and better-generalization-ability inference of the individual biological age can be realized.