97 results about "Saliva sample" patented technology

The invention relates to the technical field of oral medicine, in particular to a method for dynamically monitoring the enamel demineralization risk of an orthodontic patient based on a salivary enzyme activity spectrum, and the method comprises the following steps: S1, obtaining a saliva sample from the orthodontic patient at least twice in an orthodontic treatment process; s2, treating the saliva sample to obtain a standardized saliva sample; s3, analyzing the standardized saliva sample to obtain at least two different enzymatic characteristic indexes of at least one enzyme or enzyme system in the saliva, and reflecting multiple molecular characteristics of the enzyme or enzyme system or response of the enzyme or enzyme system to environmental change through the enzymatic characteristic indexes; and S4, dynamically evaluating the enamel demineralization risk of the orthodontic patient based on the change of the enzymological characteristic indexes obtained at different time points. By analyzing multi-dimensional molecular behaviors and dynamic changes of enzymes or enzyme systems in saliva, the method aims at overcoming the defects of the prior art in the aspects of early warning ability, dynamic nature and individualized evaluation, and early, dynamic and individualized monitoring of the enamel demineralization risk of an orthodontic patient is achieved.

The invention provides a microbial marker and a prediction model for schizophrenia with social anxiety disorder. The microbial marker is a Bacteroides microorganism in saliva. The relevance between the relative abundance of Bacteroides microorganisms in saliva and social anxiety disorder is found for the first time. A Nomogram clinical prediction mode is formed by taking the specific microbial marker as a microbial marker in combination with a children bad experience scale and 6-hydroxymelatonin and 5-hydroxyindoleacetic acid in serum, so that objective biological indexes can be provided for early diagnosis and monitoring of whether schizophrenia is accompanied by social anxiety disorder or not; errors of subjective evaluation are reduced; moreover, the oral saliva sample has the obvious advantage of being easy to collect for psychiatric patients, the working difficulty of medical staff can be greatly reduced, and revolutionary changes of prediction, diagnosis and treatment of schizophrenia accompanied with social anxiety disorder are expected to be brought.

The application discloses a method for determining 15 new psychoactive substances in saliva by solid phase supported liquid-liquid extraction (SLE) combined with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS), which specifically uses a mixed solution of methyl tert-butyl ether and n-hexane as an elution solvent, adopts solid phase supported liquid-liquid extraction to pretreat saliva samples, and then adopts UPLC-MS / MS to detect the collected eluent, so as to realize qualitative and quantitative determination of the new psychoactive substances in saliva. The method has the advantages of rapidness, accuracy, high sensitivity and simple operation, the detection limit of the method is 0.2-0.5 ng / mL, can meet the domestic and foreign limit requirements, and provides technical support for monitoring and investigation of the new psychoactive substances in saliva.

The application relates to the field of biotechnology and health informatics, and particularly discloses a microorganism marker combination for detecting obstructive sleep apnea of children and application thereof. The microorganism marker combination comprises the following nine microorganism genera: g__Streptococcus, g__Solobacterium, g__Schaalia, g__Capnocytophaga, g__Stomatobaculum, g__Mogibacterium, g__Clostridia_UCG-014, g__Selenomonas and g__Pseudoleptotrichia. By detecting the relative abundance of the above microorganism genera in a saliva sample of a child, a classification model is constructed by using a random forest algorithm, non-invasive and objective screening of OSA of the child is realized, and the application has high accuracy and clinical application value.

The present invention relates to a biosensor that enables the detection of biomarkers related to bone metabolism through saliva samples. This biosensor, which uses a saliva sampling method and offers an economical and user-friendly solution by adopting a non-invasive approach, can detect biomarkers related to bone formation and resorption such as Procollagen Type I N-terminal Propeptide (PINP) and β-C- terminal Telopeptide (β-CTX) in saliva with high sensitivity. The present invention increases patient comfort while also providing rapid analysis, making the diagnosis and treatment processes of diseases related to bone metabolism more efficient.

The invention relates to the field of disease prevention and control, in particular to an auxiliary monitoring system for epidemic disease protection. The invention provides the auxiliary monitoring system for epidemic disease protection, which can automatically collect a saliva sample of a detector, save manpower and prevent medical personnel from being infected with epidemic diseases. An auxiliary monitoring system for epidemic disease protection comprises a machine body, a small lead screw, a top cover and the like, the small lead screw is rotationally connected between the front portion and the rear portion of the machine body, and the top cover is clamped to the upper side of the machine body. According to the spittle collecting device, the spiral moving seat moves forwards to drive the safety disc to move forwards, meanwhile, the mouth of a detector is opened, a collecting cotton swab on an elbow moves to the throat of the detector, and the effects that the spittle sample of the detector can be automatically collected, manpower is saved, and medical workers are prevented from being infected with epidemic diseases are achieved.

This invention discloses a method for calculating stress age based on saliva extraction, belonging to the field of data processing technology. It includes collecting saliva samples and personal data from individuals, including individual characteristics, physiological data, and behavioral data; obtaining telomere detection data from the saliva samples; analyzing the telomere detection data, physiological data, and behavioral data to obtain telomere-related data; and generating a test report based on the telomere-related data. This method achieves the scientific quantification of emotional and psychological stress, and compared to traditional scales, the test results are more scientific and accurate.

The invention discloses a saliva biomarker and application thereof in rheumatoid arthritis disease activity assessment, and relates to the technical field of biomarkers. The saliva biomarker is one of the secretion amount of saliva extracellular vesicles and the expression level of porphyromonas gingivalis peptidyl arginine deiminase in the saliva extracellular vesicles. Peptidyl arginine deiminase exists in extracellular vesicles secreted by porphyromonas gingivalis in saliva, and in a patient with rheumatoid arthritis, the secretion amount of the extracellular vesicles in the saliva is remarkably increased and is in positive correlation with disease activity; the expression level of porphyromonas gingivalis peptidyl arginine deiminase in saliva extracellular vesicles is remarkably improved and is in positive correlation with disease activity. According to the invention, real noninvasive and high-compliance detection is realized based on a saliva sample, and large-scale screening and long-term management can be realized.

A method for diagnosing prostate cancer comprising: providing a saliva sample from a subject, and measuring the expression of C4B (complement component 4) in the sample. Also claimed are separate meth

The invention discloses a non-invasive prediction method for acne risk of puberty women and a kit, and belongs to the technical field of biological detection. According to the present invention, the non-invasive advantage of the saliva sample is adopted, and the cycle specificity of saliva crystallization and the pathological correlation of the sex hormone ratio are combined to achieve the early accurate early warning of the acne risk, such that the problems of strong invasion and difficult promotion in the teenager group of the traditional serum detection are solved, and the characteristics of convenient operation, low cost and repeatable monitoring are provided; and a new technical means is provided for prevention and early intervention of acne of puberty women.

The invention provides a method for detecting the total antioxidant capacity of saliva based on iron-based MOF nano-enzyme, and belongs to the field of biosensing. The method comprises the following steps: obtaining an MIL-53Fe nano enzyme; the preparation method comprises the following steps: mixing a diluent of MIL-53Fe nano-enzyme, a sodium acetate buffer solution, 3, 3 ', 5, 5'-tetramethyl benzidine and hydrogen peroxide for reaction, adsorbing a reaction solution by using filter paper, drying and curing by using a chitosan solution to obtain the visual microfluidic detection paper-based chip. Establishing a standard curve of the chromatic value and the ascorbic acid concentration; dropwise adding the treated saliva sample onto the paper-based chip, standing for reaction to obtain a chromatic value, and calculating according to the standard curve to obtain the total antioxidant capacity value of the saliva. Complex matrix interference is broken through, the detection sensitivity and portability are improved, and therefore clinical application of saliva TAC detection in noninvasive health monitoring is promoted.

The invention relates to the technical field of biological detection, in particular to a saliva RF-IgM rapid detection kit and application thereof, and the saliva RF-IgM rapid detection kit comprises a saliva pretreatment module, a signal amplification module and a result interpretation module. The invention provides an RF-IgM rapid detection method based on a saliva sample and application thereof, and solves the problems of invasiveness and time consumption of traditional serological detection. Technical difficulties in saliva detection, including the problems of insufficient sensitivity and high false negative rate caused by large IgM molecular weight and saliva mucoprotein interference, are solved. Non-invasive, rapid and convenient detection of rheumatoid factors is achieved, patient compliance is improved, and the kit is suitable for outpatient real-time detection (POCT), primary medical treatment and large-scale population early screening.

A method of testing a saliva sample includes: providing a testing device having a color palette and multiple test areas, using colorimetric analysis and lateral flow assay tests; collecting at least a prescribed volume of a saliva sample; dispensing, onto each of multiple test areas of a testing device, a respective portion of the collected saliva sample, the testing device thereby developing a respective discernible reaction at each test area; capturing, by a computing device, an image of testing device; analyzing, through a testing platform, the image by colorimetric and liquid flow assay analysis; and displaying results of the colorimetric analysis, the displayed results representing oral health parameters. The displayed results including quantitative indices and trend identifiers related to oral health parameters. A kit for use in conducting the above method includes the sample reactive device.

The invention discloses an ergothioneine trimethylamine lyase mutant and application of the ergothioneine trimethylamine lyase mutant in detection of histidine betaine in saliva. L-histidine betaine is catalyzed by ergothioneine trimethylamine lyase to be hydrolyzed into urocanic acid, the light absorption value of the urocanic acid at 290 nm is detected by a colorimetric method, and the content of L-histidine betaine in saliva can be determined. The method is high in sensitivity, and detection of 12.5-100 mg / L histidine betaine in a saliva sample can be completed within 1.5 hours. The method is simple and convenient to operate and can be used for quickly judging the metabolic state of human ergothioneine.

Device for the visual detection of analytes, in particular HCG, in a liquid sample originating from the human or animal organism, in particular a urine or saliva sample, comprising a carrier body (1) which receives at least one test strip (3) provided with a sample receiving section (32) and a test section (34), characterized in that a slider (2) is arranged which is mounted so as to be displaceable in the longitudinal direction of the carrier body (1) and which, in at least one sliding position, projects beyond the carrier body (1) in an extended manner, wherein at least the carrier body (1) is made of biodegradable material, in particular of a paper material.

The utility model provides a saliva sampling detection device which comprises a detection head, a drainage component, a locking part and a saliva storage component, a threaded head is installed on one of the detection head and the drainage component, and a threaded hole is formed in the other one of the detection head and the drainage component; the locking part comprises a limiting piece and a buckling piece, the limiting piece is installed on one of the detection head and the drainage component, and the buckling piece is installed on the other one of the detection head and the drainage component; the fastener comprises an intercepting surface, and when the limiting part makes contact with the intercepting surface, the detection head rotates in one direction relative to the drainage component. The detection head rotates forwards relative to the drainage component and is gradually screwed and connected, the limiting piece rotates relative to the drainage component along with the detection head, at the moment, the limiting piece does not make contact with the interception face of the fastener, and if the detection head rotates backwards relative to the drainage component, when the detection head and the limiting piece rotate by at most 360 degrees relative to the drainage component, the limiting piece makes contact with the interception face, and the detection head is stopped. The interception face prevents the limiting piece from rotating relative to the drainage component, and therefore separation of the detection head and the drainage component is limited.

The invention discloses an esophageal squamous cell carcinoma noninvasive screening system based on a multi-marker saliva microbiome, and relates to the technical field of microorganisms and oncology, the key points of the technical scheme are as follows: the system comprises an input module, an analysis module and an output module, the input module inputs abundance change of specific flora of a saliva sample needing to be screened; the analysis module is used for analyzing the input saliva sample flora data; and the output module outputs the analysis result of the analysis module and judges whether the screened saliva sample owner belongs to the high-risk population of the esophageal squamous cell carcinoma patient. The abundance change of the specific flora of the saliva sample is obtained through metagenome sequencing or targeted PCR detection, and a microbial characteristic model is constructed to distinguish high-risk people. By means of the system, early-stage, non-invasive and high-acceptability screening of esophageal squamous carcinoma can be achieved.

A method for processing a saliva sample for downstream DNA analysis. The method includes mixing the saliva sample with a chelating resin solution to form a mixture, incubating the mixture at a temperature of 120° C. or lower and duration of at least 1 minute, wherein the temperature and duration are inversely related, shaking the mixture before and after incubation for a period ranging from 0 seconds to 2 hours, separating the mixture into a supernatant and a sedimented chelating resin using either centrifugation or by allowing natural gravitational settling, and recovering the supernatant for subsequent analysis.

The utility model discloses a portable saliva test box, and belongs to the field of saliva sample collection, the portable saliva test box comprises a test box, the top end of the test box is provided with a sealing cover, the bottom surface of the sealing cover is provided with a sampling rod, the bottom end of the sampling rod is provided with a sampling head, one side of the top surface of the test box is provided with a detection port, and the inner side surface of the test box is provided with a through port; and the through opening is communicated with the detection opening. The method has the effect of improving the accuracy of the detection result of the saliva sample.

The application discloses a method for screening metabolic biomarkers based on saliva metabolic fingerprint and application, and relates to the technical field of biological sample metabolic analysis. The method comprises the following steps: step 1, inorganic nanoparticles are used to enhance laser desorption ionization time-of-flight mass spectrometry technology, saliva samples of Parkinson's disease patients and healthy volunteers are subjected to metabolic detection, and saliva metabolic fingerprints are obtained; step 2, the saliva metabolic fingerprints obtained in step 1 are subjected to a machine learning algorithm, saliva samples are subjected to a variety of model training, a variety of models are evaluated through a cross-validation method, and a final prediction model is screened according to a verification result; step 3, saliva samples of Parkinson's disease patients and healthy volunteers are predicted according to the prediction model of step 2, a prediction result is obtained, and metabolic biomarkers in the saliva samples are screened according to the prediction result. The application has high screening efficiency, the result is accurate and reliable, and the screened metabolic biomarkers can be used for prediction of Parkinson's disease.

The application discloses a nucleic acid absolute quantification method based on nucleic acid constant temperature amplification, which comprises the following specific steps: step one, sample pretreatment, blood samples and saliva samples are pretreated respectively; step two, reaction system configuration and assembly; step three, digital micro-reaction unit preparation and amplification; and step four, multiple signal analysis and absolute quantification. In the application, multiple primer design and a double-probe system are adopted, so that the mutation detection rate is improved, and the detection limit can be reduced to 0.1% variation through Cas12a-sgRNA auxiliary verification. The double-emulsification technology of the micro-fluidic chip is adopted, so that the droplet diameter variation coefficient is controlled within 5%, and the clogged micropore rate is reduced by real-time analysis of the micropore array image through the ImageJ software, automatic marking and exclusion of abnormal units.

The utility model discloses an HIV (human immunodeficiency virus) self-test saliva detection box which comprises a detection box body, the upper end of the detection box body is provided with a developing area and a sample hole, the end face and the side wall of the detection box body are respectively provided with two groups of positioning grooves and fixing grooves, the positioning grooves and the fixing grooves are mutually symmetrical in position, and a positioning plate is clamped in the two groups of fixing grooves together. The outer wall of the positioning plate is in a U shape, a positioning hole is formed in the center point of the positioning plate in a penetrating mode, the U-shaped inner wall of the positioning plate makes contact with the end faces of the two positioning blocks, and a fixing device for fixing the positioning plate is installed on the side wall of the detection box body. By arranging the positioning plate and the positioning hole, a saliva sample can accurately enter the central area of the sample hole along a vertical path, so that the sample is prevented from being diffused or dropped to the surrounding area due to dropping position deviation, the accuracy of a detection result is improved, the operation error rate is reduced, and the detection efficiency is improved. And the anxiety emotion caused by tension or unskilled feeling of the user is relieved.

Provided herein are fluid sample collection devices, particularly, saliva sample collection devices, and methods of using them. In certain embodiments, the fluid sample collection device comprises: a first portion and a second portion, the two portions removably joined together, wherein each of the first portion and the second portion comprises a casing configured to removably accommodate an adsorbent sample collection pad. Each adsorbent sample collection pad comprises a sample collection element and a longitudinal element, which fits in a longitudinal groove of the casing. The casings removably connect with each other via connection elements to form the fluid sample collection device. In some embodiments, the adsorbent sample collection pad comprises an indicator dye, which provides a signal when contacted with a sample. In some cases, the first and / or the second portion comprise a holding element for a user. Methods of collecting saliva samples using the collection devices are also described.

The utility model relates to a single-gene genetic disease detection and screening device which comprises a tube body and a tube cover which cover each other, and the tube body is in threaded connection with the tube cover; the tube body is used for accommodating a sample preservation solution, and a breaking groove is formed in the side wall of the tube cover and is used for accommodating a swab; when the groove bottom wall of the breaking groove is used for abutting against the breaking point of the swab, the tube body is inserted into the tube cover. In the application, the groove is broken from the side wall of the tube cover, so that the tube cover and the tube body clamp the swab, and the swab is broken; part of the tube body is inserted into the tube cover, so that a user can conveniently hold the tube body and the tube body. When the swab is broken off by a user, the risk that the tube body, the tube cover and the swab are separated from each other can be reduced, so that a childbearing-age couple can sample saliva conveniently, and detection and screening of single-gene genetic diseases are carried out.

An object of the present invention is to provide a reagent capable of reducing a non-specific reaction in the detection of a substance to be measured in a saliva sample using Substance 1 that is labeled with a peroxidase and that binds to the substance to be measured, a kit including the reagent, and a method for measuring a substance to be measured using the reagent. According to the present invention, there are provided a reagent containing a glycoprotein, in which, in detection of a substance to be measured in a saliva sample using Substance 1 that is labeled with peroxidase and binds to the substance to be measured, the reagent is a reagent for being brought into contact with the saliva sample prior to contact between the Substance 1 and the substance to be measured; a kit containing the reagent; and a method for measuring of a substance to be measured using the reagent.

This invention relates to the field of biotechnology, specifically to a preservation solution and its preparation method for preserving human cellular nucleic acid DNA and RNA in saliva samples at both room temperature and 60°C. The preservation solution of this invention, capable of preserving human cellular nucleic acid DNA and RNA in saliva samples at both room temperature and 60°C, comprises the following components: 1,2-cyclohexanediaminetetraacetic acid (CDTA), hydroxyethylpiperazine-2-ethanesulfonic acid, SDS, sodium chloride, ethanol, and Tween-20. This invention offers the following technical advantages: The saliva DNA & RNA preservation solution provided by this invention can stably preserve nucleic acid DNA in saliva samples at 37°C for at least 2 years. The saliva DNA & RNA preservation solution provided by this invention can stably preserve nucleic acid in saliva samples at 37°C for at least 30 days. The saliva DNA & RNA preservation solution provided by this invention can stably preserve nucleic acid in saliva samples at 60°C for at least 7 days, facilitating sample transport to third-party testing institutions for analysis.

A method and composition for the simultaneous detection of a protein and a nucleic acid within a single biological sample using proximity ligation of aptamers and quantitative polymerase chain reaction (qPCR) is disclosed. The invention employs two aptamers, each targeting a distinct epitope of the protein of interest, functionalized with primer sites to enable ligation by way of a short DNA linker in the presence of the protein. This ligated construct is then amplified and detected using qPCR. Concurrently, viral RNA is detected in the same reaction using reverse transcription- qPCR (RT-qPCR) with TaqMan probes in a separate fluorescent channel. This dual detection approach is demonstrated with SARS-CoV-2 nucleocapsid protein and RNA and is validated in saliva samples and digital droplet PCR. The invention provides a sensitive, rapid, and specific method for multiplexed molecular diagnostics.

The invention relates to a method for identifying an emotion or a group of emotions felt by a subject after olfactory stimulation, from saliva samples of said subject implementing salivary biomarkers signature. In a second aspect, the invention also relates to a method for evaluating the capacity of an olfactory stimulus to provoke in a subject a desired emotion or group of emotions. The invention finally relates to the use of kit in the context of the present invention.

The utility model relates to an anti-pollution saliva collection detector, and relates to the field of saliva detectors. The device comprises a detection piece and a sampling piece, the detection piece comprises a shell, the bottom surface of the shell is open, a bottom plate is assembled at the open end of the shell, a mounting groove is formed in the top surface of the bottom plate, a detection card is horizontally placed in the mounting groove, a funnel-shaped sample adding hole is formed in one side of the top surface of the shell in a penetrating manner, and the bottom surface of the shell is provided with an opening; a display hole is formed in the other side of the top surface of the shell in a penetrating manner, and the display hole corresponds to the detection card display area. The capacity of the sample adding hole is large, enough saliva samples to be detected can be collected, meanwhile, the situation that the saliva samples overflow during detection is avoided, and the detection result is affected by sample pollution possibly caused by sample overflow which pollutes test paper in other areas or makes contact with a detector shell; the hidden danger of sample overflow is solved by an enlarged containing area of a liquid collecting area of the sample adding hole and a funnel-shaped hole.

Disclosed is an in vitro method for assessing whether a human subject has periodontitis. The method comprises detecting, in a sample of saliva from said subject, the concentrations of the proteins Hepatocyte Growth Factor (HGF) and Matrix Metalloproteinase 8 (MMP-8). Based on the concentrations determined, and adding age, and possibly other demographic markers such as sex and / or BMI, a testing value reflecting the joint concentrations is determined for said proteins, in combination with one or more demographic markers. The testing value is compared with a threshold value. The threshold reflects in the same manner the joint concentrations and the age, and possibly other demographic markers, as associated with periodontitis and may be seen as an upper limit of testing values as seen in a population of subjects without periodontitis. Thereby a testing value at or above the threshold value is indicative for periodontitis in said subject.