134 results about "Saliva sample" patented technology

The invention relates to a composition for identifying oral squamous cell carcinoma based on saliva sample detection and application, and belongs to the technical field of molecular biology. The composition provided by the invention is used for identifying the oral squamous cell carcinoma by detecting the expression of SNORD52 and / or SNORD65 in a saliva sample; the composition comprises a specific reverse transcription stem-loop primer, a specific detection forward primer, a specific detection probe and a universal reverse primer. According to the composition disclosed by the invention, the expression of SNORD52 and / or SNORD65 in a saliva sample is detected, and a binomial logistic regression model is combined to perform auxiliary judgment on the oral squamous cell carcinoma, so that the composition has relatively high sensitivity and specificity, and the identification accuracy of the oral squamous cell carcinoma can be improved. The invention further provides a kit containing the composition, a detection object of the kit is a saliva sample, and the kit has the advantages of being small in sample dosage, noninvasive in sampling, convenient, rapid, small in risk and the like.

The invention relates to a saliva sample extractor for the non-invasive collection of saliva samples, particularly from infants. The saliva sample extractor is designed as a pacifier and comprises a teat (1) formed as a shape-elastic bladder with an inner cavity (7) for saliva collection. The wall of the teat (1) has at least one wall section made of a fluid-selectively permeable membrane (6), through which saliva fluid (8) can pass into the inner cavity (7). The teat (1) also has a vent opening closed by a one-way valve (5). Saliva collection occurs passively through natural sucking and chewing movements. The saliva sample extractor ensures hygienic, stress-free, and efficient saliva collection, which is particularly suitable for infants and uncooperative patient groups.

The invention discloses a plasma concentration detection method, and belongs to the field of biomedical detection.The plasma concentration detection method comprises the steps that a saliva sample and a mesenchymal fluid sample of a to-be-detected object are synchronously collected through non-invasive equipment and minimally invasive equipment, and after centrifugation and membrane filtration pretreatment, the saliva sample and the mesenchymal fluid sample are obtained; a multi-channel biosensor is used for detecting drug metabolite concentrations in saliva and interstitial fluid respectively, a blood drug concentration predicted value is further calculated through a composite calibration model, the model is constructed based on a random forest algorithm, saliva and interstitial fluid concentration data are input, the blood drug concentration is output, and dynamic correction is conducted through species characteristic parameters. Wherein species parameters are obtained in real time through near infrared spectrum, weight coefficients (alpha and beta) are optimized in combination with a multiple regression equation, and finally high-precision prediction is achieved through a weighting algorithm. The method has the advantages of non-invasiveness, cross-species universality, dynamic adaptive correction and the like, and is suitable for clinical monitoring, veterinary medicine and pharmacokinetic research.

The invention relates to the technical field of oral medicine, in particular to a method for dynamically monitoring the enamel demineralization risk of an orthodontic patient based on a salivary enzyme activity spectrum, and the method comprises the following steps: S1, obtaining a saliva sample from the orthodontic patient at least twice in an orthodontic treatment process; s2, treating the saliva sample to obtain a standardized saliva sample; s3, analyzing the standardized saliva sample to obtain at least two different enzymatic characteristic indexes of at least one enzyme or enzyme system in the saliva, and reflecting multiple molecular characteristics of the enzyme or enzyme system or response of the enzyme or enzyme system to environmental change through the enzymatic characteristic indexes; and S4, dynamically evaluating the enamel demineralization risk of the orthodontic patient based on the change of the enzymological characteristic indexes obtained at different time points. By analyzing multi-dimensional molecular behaviors and dynamic changes of enzymes or enzyme systems in saliva, the method aims at overcoming the defects of the prior art in the aspects of early warning ability, dynamic nature and individualized evaluation, and early, dynamic and individualized monitoring of the enamel demineralization risk of an orthodontic patient is achieved.

The invention relates to a saliva specimen collection technology, and discloses a saliva collection device, a collector of the saliva collection device comprises a horseshoe-shaped collector body matched with a sublingual area, a sublingual matched support body is formed by a middle concave area of the collector body and an upper shell and used for supporting a tongue belly, and a tongue frenum receding opening is formed in the front portion of the collector body. A negative pressure liquid collecting chamber and a drainage connecting pipe are arranged in the collector body, the tongue frenum receding opening is communicated with the middle concave area, two collecting holes symmetrically formed along the tongue frenum receding opening are formed in the front side of a base body at the bottom of the collector body, a flow guide opening is formed in the negative pressure liquid collecting chamber, and a sealing plug for blocking the flow guide opening is arranged at the tail of the collector body. And the two collecting holes are respectively communicated with the negative pressure liquid collecting chamber through drainage connecting pipes. The saliva collector is arranged at the tongue base to automatically collect saliva, a saliva sample does not make contact with the surrounding external environment from secretion to collection, the collection process is safe, efficient and convenient, the saliva sample is free of loss and pollution, and the saliva collector is particularly suitable for saliva sample collection of acute RIOM patients.

The invention provides a microbial marker and a prediction model for schizophrenia with social anxiety disorder. The microbial marker is a Bacteroides microorganism in saliva. The relevance between the relative abundance of Bacteroides microorganisms in saliva and social anxiety disorder is found for the first time. A Nomogram clinical prediction mode is formed by taking the specific microbial marker as a microbial marker in combination with a children bad experience scale and 6-hydroxymelatonin and 5-hydroxyindoleacetic acid in serum, so that objective biological indexes can be provided for early diagnosis and monitoring of whether schizophrenia is accompanied by social anxiety disorder or not; errors of subjective evaluation are reduced; moreover, the oral saliva sample has the obvious advantage of being easy to collect for psychiatric patients, the working difficulty of medical staff can be greatly reduced, and revolutionary changes of prediction, diagnosis and treatment of schizophrenia accompanied with social anxiety disorder are expected to be brought.

The application discloses a method for determining 15 new psychoactive substances in saliva by solid phase supported liquid-liquid extraction (SLE) combined with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS), which specifically uses a mixed solution of methyl tert-butyl ether and n-hexane as an elution solvent, adopts solid phase supported liquid-liquid extraction to pretreat saliva samples, and then adopts UPLC-MS / MS to detect the collected eluent, so as to realize qualitative and quantitative determination of the new psychoactive substances in saliva. The method has the advantages of rapidness, accuracy, high sensitivity and simple operation, the detection limit of the method is 0.2-0.5 ng / mL, can meet the domestic and foreign limit requirements, and provides technical support for monitoring and investigation of the new psychoactive substances in saliva.

The application relates to the field of biotechnology and health informatics, and particularly discloses a microorganism marker combination for detecting obstructive sleep apnea of children and application thereof. The microorganism marker combination comprises the following nine microorganism genera: g__Streptococcus, g__Solobacterium, g__Schaalia, g__Capnocytophaga, g__Stomatobaculum, g__Mogibacterium, g__Clostridia_UCG-014, g__Selenomonas and g__Pseudoleptotrichia. By detecting the relative abundance of the above microorganism genera in a saliva sample of a child, a classification model is constructed by using a random forest algorithm, non-invasive and objective screening of OSA of the child is realized, and the application has high accuracy and clinical application value.

The present invention relates to a biosensor that enables the detection of biomarkers related to bone metabolism through saliva samples. This biosensor, which uses a saliva sampling method and offers an economical and user-friendly solution by adopting a non-invasive approach, can detect biomarkers related to bone formation and resorption such as Procollagen Type I N-terminal Propeptide (PINP) and β-C- terminal Telopeptide (β-CTX) in saliva with high sensitivity. The present invention increases patient comfort while also providing rapid analysis, making the diagnosis and treatment processes of diseases related to bone metabolism more efficient.

The invention relates to the field of disease prevention and control, in particular to an auxiliary monitoring system for epidemic disease protection. The invention provides the auxiliary monitoring system for epidemic disease protection, which can automatically collect a saliva sample of a detector, save manpower and prevent medical personnel from being infected with epidemic diseases. An auxiliary monitoring system for epidemic disease protection comprises a machine body, a small lead screw, a top cover and the like, the small lead screw is rotationally connected between the front portion and the rear portion of the machine body, and the top cover is clamped to the upper side of the machine body. According to the spittle collecting device, the spiral moving seat moves forwards to drive the safety disc to move forwards, meanwhile, the mouth of a detector is opened, a collecting cotton swab on an elbow moves to the throat of the detector, and the effects that the spittle sample of the detector can be automatically collected, manpower is saved, and medical workers are prevented from being infected with epidemic diseases are achieved.

This invention discloses a method for calculating stress age based on saliva extraction, belonging to the field of data processing technology. It includes collecting saliva samples and personal data from individuals, including individual characteristics, physiological data, and behavioral data; obtaining telomere detection data from the saliva samples; analyzing the telomere detection data, physiological data, and behavioral data to obtain telomere-related data; and generating a test report based on the telomere-related data. This method achieves the scientific quantification of emotional and psychological stress, and compared to traditional scales, the test results are more scientific and accurate.

The invention discloses a saliva biomarker and application thereof in rheumatoid arthritis disease activity assessment, and relates to the technical field of biomarkers. The saliva biomarker is one of the secretion amount of saliva extracellular vesicles and the expression level of porphyromonas gingivalis peptidyl arginine deiminase in the saliva extracellular vesicles. Peptidyl arginine deiminase exists in extracellular vesicles secreted by porphyromonas gingivalis in saliva, and in a patient with rheumatoid arthritis, the secretion amount of the extracellular vesicles in the saliva is remarkably increased and is in positive correlation with disease activity; the expression level of porphyromonas gingivalis peptidyl arginine deiminase in saliva extracellular vesicles is remarkably improved and is in positive correlation with disease activity. According to the invention, real noninvasive and high-compliance detection is realized based on a saliva sample, and large-scale screening and long-term management can be realized.

A method for diagnosing prostate cancer comprising: providing a saliva sample from a subject, and measuring the expression of C4B (complement component 4) in the sample. Also claimed are separate meth

The invention discloses a non-invasive prediction method for acne risk of puberty women and a kit, and belongs to the technical field of biological detection. According to the present invention, the non-invasive advantage of the saliva sample is adopted, and the cycle specificity of saliva crystallization and the pathological correlation of the sex hormone ratio are combined to achieve the early accurate early warning of the acne risk, such that the problems of strong invasion and difficult promotion in the teenager group of the traditional serum detection are solved, and the characteristics of convenient operation, low cost and repeatable monitoring are provided; and a new technical means is provided for prevention and early intervention of acne of puberty women.

The invention provides a method for detecting the total antioxidant capacity of saliva based on iron-based MOF nano-enzyme, and belongs to the field of biosensing. The method comprises the following steps: obtaining an MIL-53Fe nano enzyme; the preparation method comprises the following steps: mixing a diluent of MIL-53Fe nano-enzyme, a sodium acetate buffer solution, 3, 3 ', 5, 5'-tetramethyl benzidine and hydrogen peroxide for reaction, adsorbing a reaction solution by using filter paper, drying and curing by using a chitosan solution to obtain the visual microfluidic detection paper-based chip. Establishing a standard curve of the chromatic value and the ascorbic acid concentration; dropwise adding the treated saliva sample onto the paper-based chip, standing for reaction to obtain a chromatic value, and calculating according to the standard curve to obtain the total antioxidant capacity value of the saliva. Complex matrix interference is broken through, the detection sensitivity and portability are improved, and therefore clinical application of saliva TAC detection in noninvasive health monitoring is promoted.

The invention relates to the technical field of biological detection, in particular to a saliva RF-IgM rapid detection kit and application thereof, and the saliva RF-IgM rapid detection kit comprises a saliva pretreatment module, a signal amplification module and a result interpretation module. The invention provides an RF-IgM rapid detection method based on a saliva sample and application thereof, and solves the problems of invasiveness and time consumption of traditional serological detection. Technical difficulties in saliva detection, including the problems of insufficient sensitivity and high false negative rate caused by large IgM molecular weight and saliva mucoprotein interference, are solved. Non-invasive, rapid and convenient detection of rheumatoid factors is achieved, patient compliance is improved, and the kit is suitable for outpatient real-time detection (POCT), primary medical treatment and large-scale population early screening.

A method of testing a saliva sample includes: providing a testing device having a color palette and multiple test areas, using colorimetric analysis and lateral flow assay tests; collecting at least a prescribed volume of a saliva sample; dispensing, onto each of multiple test areas of a testing device, a respective portion of the collected saliva sample, the testing device thereby developing a respective discernible reaction at each test area; capturing, by a computing device, an image of testing device; analyzing, through a testing platform, the image by colorimetric and liquid flow assay analysis; and displaying results of the colorimetric analysis, the displayed results representing oral health parameters. The displayed results including quantitative indices and trend identifiers related to oral health parameters. A kit for use in conducting the above method includes the sample reactive device.

The invention relates to the technical field of biological sample collection, and provides saliva sample collection equipment which comprises a storage box and a sealing cover, a frame is arranged on the inner wall of the storage box, a storage block, a disinfection box and a storage shell are fixedly connected to the inner wall of the frame, a plurality of storage grooves are formed in the upper surface of the storage block, and the storage grooves are communicated with the storage box. Test tubes are arranged on the inner walls of the multiple storage grooves, bouncing pieces are arranged at the bottoms in the multiple storage grooves, and a cooling component is arranged below the storage block. The disinfection device is reasonable in structure, a flow guide cover needing to be cleaned and disinfected is placed in the disinfection box, a sealing plug is opened, disinfectant is added into the liquid box through a liquid inlet, the disinfectant enters a spray head through a water conveying pipe by means of a water pump, the flow guide cover is cleaned and disinfected through the spray head, disinfection treatment on the flow guide cover is achieved, and secondary use is facilitated.

The present invention is applicable to the field of biomedical technologies and provides the use of EHD3 and ATP6V1E1 as markers for predicting the progression of periodontal disease and therapeutic targets. The present invention collects non-invasive and easily obtainable saliva samples from healthy individuals and patients clinically diagnosed with mild, moderate, and severe periodontitis, purifies the exosomes therein, and conducts proteomic sequencing studies. During the research process, the purified exosomes are identified, and then proteomic detection is carried out. By deeply analyzing the proteomic data, the present invention discovers two key proteins, namely EHD3 and ATP6V1E1, whose expression levels continuously increase as periodontitis progresses from mild to moderate to severe. These two proteins can accurately reflect the process of periodontal disease, and thus can be used as predictive and diagnostic biomarkers for the progression of different stages of periodontal disease, and also provide potential targets for the effective treatment of periodontal disease.

The invention discloses an ergothioneine trimethylamine lyase mutant and application of the ergothioneine trimethylamine lyase mutant in detection of histidine betaine in saliva. L-histidine betaine is catalyzed by ergothioneine trimethylamine lyase to be hydrolyzed into urocanic acid, the light absorption value of the urocanic acid at 290 nm is detected by a colorimetric method, and the content of L-histidine betaine in saliva can be determined. The method is high in sensitivity, and detection of 12.5-100 mg / L histidine betaine in a saliva sample can be completed within 1.5 hours. The method is simple and convenient to operate and can be used for quickly judging the metabolic state of human ergothioneine.

Device for the visual detection of analytes, in particular HCG, in a liquid sample originating from the human or animal organism, in particular a urine or saliva sample, comprising a carrier body (1) which receives at least one test strip (3) provided with a sample receiving section (32) and a test section (34), characterized in that a slider (2) is arranged which is mounted so as to be displaceable in the longitudinal direction of the carrier body (1) and which, in at least one sliding position, projects beyond the carrier body (1) in an extended manner, wherein at least the carrier body (1) is made of biodegradable material, in particular of a paper material.

The utility model provides a saliva sampling detection device which comprises a detection head, a drainage component, a locking part and a saliva storage component, a threaded head is installed on one of the detection head and the drainage component, and a threaded hole is formed in the other one of the detection head and the drainage component; the locking part comprises a limiting piece and a buckling piece, the limiting piece is installed on one of the detection head and the drainage component, and the buckling piece is installed on the other one of the detection head and the drainage component; the fastener comprises an intercepting surface, and when the limiting part makes contact with the intercepting surface, the detection head rotates in one direction relative to the drainage component. The detection head rotates forwards relative to the drainage component and is gradually screwed and connected, the limiting piece rotates relative to the drainage component along with the detection head, at the moment, the limiting piece does not make contact with the interception face of the fastener, and if the detection head rotates backwards relative to the drainage component, when the detection head and the limiting piece rotate by at most 360 degrees relative to the drainage component, the limiting piece makes contact with the interception face, and the detection head is stopped. The interception face prevents the limiting piece from rotating relative to the drainage component, and therefore separation of the detection head and the drainage component is limited.

The invention discloses an esophageal squamous cell carcinoma noninvasive screening system based on a multi-marker saliva microbiome, and relates to the technical field of microorganisms and oncology, the key points of the technical scheme are as follows: the system comprises an input module, an analysis module and an output module, the input module inputs abundance change of specific flora of a saliva sample needing to be screened; the analysis module is used for analyzing the input saliva sample flora data; and the output module outputs the analysis result of the analysis module and judges whether the screened saliva sample owner belongs to the high-risk population of the esophageal squamous cell carcinoma patient. The abundance change of the specific flora of the saliva sample is obtained through metagenome sequencing or targeted PCR detection, and a microbial characteristic model is constructed to distinguish high-risk people. By means of the system, early-stage, non-invasive and high-acceptability screening of esophageal squamous carcinoma can be achieved.

A method for processing a saliva sample for downstream DNA analysis. The method includes mixing the saliva sample with a chelating resin solution to form a mixture, incubating the mixture at a temperature of 120° C. or lower and duration of at least 1 minute, wherein the temperature and duration are inversely related, shaking the mixture before and after incubation for a period ranging from 0 seconds to 2 hours, separating the mixture into a supernatant and a sedimented chelating resin using either centrifugation or by allowing natural gravitational settling, and recovering the supernatant for subsequent analysis.

The utility model discloses a portable saliva test box, and belongs to the field of saliva sample collection, the portable saliva test box comprises a test box, the top end of the test box is provided with a sealing cover, the bottom surface of the sealing cover is provided with a sampling rod, the bottom end of the sampling rod is provided with a sampling head, one side of the top surface of the test box is provided with a detection port, and the inner side surface of the test box is provided with a through port; and the through opening is communicated with the detection opening. The method has the effect of improving the accuracy of the detection result of the saliva sample.

The application discloses a method for screening metabolic biomarkers based on saliva metabolic fingerprint and application, and relates to the technical field of biological sample metabolic analysis. The method comprises the following steps: step 1, inorganic nanoparticles are used to enhance laser desorption ionization time-of-flight mass spectrometry technology, saliva samples of Parkinson's disease patients and healthy volunteers are subjected to metabolic detection, and saliva metabolic fingerprints are obtained; step 2, the saliva metabolic fingerprints obtained in step 1 are subjected to a machine learning algorithm, saliva samples are subjected to a variety of model training, a variety of models are evaluated through a cross-validation method, and a final prediction model is screened according to a verification result; step 3, saliva samples of Parkinson's disease patients and healthy volunteers are predicted according to the prediction model of step 2, a prediction result is obtained, and metabolic biomarkers in the saliva samples are screened according to the prediction result. The application has high screening efficiency, the result is accurate and reliable, and the screened metabolic biomarkers can be used for prediction of Parkinson's disease.

The application discloses a nucleic acid absolute quantification method based on nucleic acid constant temperature amplification, which comprises the following specific steps: step one, sample pretreatment, blood samples and saliva samples are pretreated respectively; step two, reaction system configuration and assembly; step three, digital micro-reaction unit preparation and amplification; and step four, multiple signal analysis and absolute quantification. In the application, multiple primer design and a double-probe system are adopted, so that the mutation detection rate is improved, and the detection limit can be reduced to 0.1% variation through Cas12a-sgRNA auxiliary verification. The double-emulsification technology of the micro-fluidic chip is adopted, so that the droplet diameter variation coefficient is controlled within 5%, and the clogged micropore rate is reduced by real-time analysis of the micropore array image through the ImageJ software, automatic marking and exclusion of abnormal units.

The invention provides a novel coronavirus antigen detection kit based on immunochromatography, the kit is a detection reagent card comprising a detection reagent strip, a sample collection pad and a plastic card shell, the detection reagent strip is prepared by sequentially pasting a coated NC film, a combination pad, a sample pad and absorbent paper on a PVC backboard, a sample pad of the detection reagent card is pretreated by using 50 to 200mM of Tris-HCL with the pH value of 9.0, 1.5 to 4 percent of NaCl, 0.2 to 0.4 percent of SDS (Sodium Dodecyl Sulfate), 3 to 5 percent of BSA (Bovine Serum Albumin) and 0.2 to 1 percent of Tween-20; and labeling the novel coronavirus antibody by using latex microspheres. On the basis of combination of an immunochromatography method and a latex microsphere labeling process, the N protein antigen of the novel coronavirus in nasopharynx swabs, throat swabs, nose swabs and saliva samples is detected, and the kit can be applied to diagnosis of the novel coronavirus, self-screening and self-detection of suspected personnel and the like.

The utility model discloses an HIV (human immunodeficiency virus) self-test saliva detection box which comprises a detection box body, the upper end of the detection box body is provided with a developing area and a sample hole, the end face and the side wall of the detection box body are respectively provided with two groups of positioning grooves and fixing grooves, the positioning grooves and the fixing grooves are mutually symmetrical in position, and a positioning plate is clamped in the two groups of fixing grooves together. The outer wall of the positioning plate is in a U shape, a positioning hole is formed in the center point of the positioning plate in a penetrating mode, the U-shaped inner wall of the positioning plate makes contact with the end faces of the two positioning blocks, and a fixing device for fixing the positioning plate is installed on the side wall of the detection box body. By arranging the positioning plate and the positioning hole, a saliva sample can accurately enter the central area of the sample hole along a vertical path, so that the sample is prevented from being diffused or dropped to the surrounding area due to dropping position deviation, the accuracy of a detection result is improved, the operation error rate is reduced, and the detection efficiency is improved. And the anxiety emotion caused by tension or unskilled feeling of the user is relieved.

Provided herein are fluid sample collection devices, particularly, saliva sample collection devices, and methods of using them. In certain embodiments, the fluid sample collection device comprises: a first portion and a second portion, the two portions removably joined together, wherein each of the first portion and the second portion comprises a casing configured to removably accommodate an adsorbent sample collection pad. Each adsorbent sample collection pad comprises a sample collection element and a longitudinal element, which fits in a longitudinal groove of the casing. The casings removably connect with each other via connection elements to form the fluid sample collection device. In some embodiments, the adsorbent sample collection pad comprises an indicator dye, which provides a signal when contacted with a sample. In some cases, the first and / or the second portion comprise a holding element for a user. Methods of collecting saliva samples using the collection devices are also described.