32 results about "Lateral flow immunoassay" patented technology

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

The disclosure relates to a point-of-care test device for diagnosis and detection of bacterial infections and hidden septic processes in samples of bodily fluids of premature and newborns comprising a quantitative or semi-quantitative lateral flow immunoassay unit adapted for parallel detection of proteins of the S100 family and for several detection of increased amounts of S100A12 and calprotectin in blood, serum, plasma, and saliva.

The present invention relates to multiplex lateral flow immunochromatographic diagnostic devices configured to detect immune activity biomarkers in biological samples. The device is configured to detect granulysin as a biomarker indicative of cytotoxic immune effector activity and at least one secondary biomarker indicative of tissue-associated immune injury. In certain embodiments, the device comprises a sample pad, a conjugate pad containing labeled detection antibodies, a porous membrane comprising spatially distinct capture zones corresponding to granulysin and the secondary biomarker, a control zone, and an absorbent pad configured to facilitate capillary flow. Detection of granulysin and the secondary biomarker within a single multiplex device enables determination of immune activation state and tissue-associated immune context from a single biological sample. The device is suitable for rapid diagnostic use, including point-of-care and laboratory environments, using whole blood, plasma, serum, or related biological fluids.

The present disclosure is directed to methods for fabricating cellulose-based microneedles with exceptional wicking properties which can be used for rapid extraction of dermal interstitial fluid (ISF). Microneedle arrays are fabricated through a combination of compression molding and laser etching, utilizing microcrystalline cellulose, a biocompatible material with excellent wicking properties. These microneedles are mechanically robust and can autonomously extract fluid from the skin without the need for external instruments. As such, they can be directly integrated with paper-based lateral flow immunoassays for point-of-care disease detection.

A device for detecting and / or quantifying functional C1-esterase inhibitor (fC1-INH), the device comprising: (i) a conjugate pad comprising a first zone and a second zone, on which a first agent and a second agent are immobilized, respectively, and (ii) a membrane, which is in communication with the conjugate pad, wherein the membrane comprises a third zone, on which a third agent is immobilized. The conjugate pad may further comprise a fourth zone for placing a biological sample, which flows through the device in the order of the first zone, the second zone, and the third zone.

An embodiment relates to a reagent buffer comprising a zwitterionic sulfonic solution, a non-ionic surfactant solution, and a buffer solution, wherein the reagent buffer has a pH range of about 7.0 to about 8.0 and a circular dichroism with an absorbance peak more than 0 at 190 nm and 225 nm. In an embodiment, the reagent buffer is configured to be a reagent buffer for a lateral flow immunoassay for detection of COVID-19.

The application relates to the technical field of food safety detection, and discloses a pathogenic bacteria multi-modal detection method based on a nano-composite material and a neural network, which comprises the following steps: preparing a nano-composite material based on the metal chelating property and interface regulation technology of cuttlefish ink source nanoparticles; constructing a dual-mode immunosensing detection platform based on the nano-composite material and a lateral flow immunoassay detection platform; preliminarily detecting pathogenic bacteria based on the dual-mode immunosensing detection platform to obtain dual-mode detection signals; extracting multi-modal signal characteristics of the dual-mode detection signals based on a fusion architecture of a multilayer perceptron and a radial basis function; and analyzing the multi-modal signal characteristics based on a neural network algorithm and outputting detection results and prediction results of the pathogenic bacteria. The application combines nano-composite engineering, multi-modal signal transduction and machine learning organically, breaks through the bottleneck of traditional detection technology, and realizes dual-mode detection of rapid and accurate detection and risk assessment of foodborne pathogenic bacteria.

The application discloses a fluorescent lateral flow immunoassay method and preparation and application of a test strip thereof, and the AuNCs-LFA test strip comprises a sample pad, a gold label pad, an NC membrane, an absorption pad and a PVC bottom plate; wherein the NC membrane comprises a signal probe AuNCs-Ab, an antigen ZEN-BSA and a goat anti-mouse lgG. The application provides a fluorescent LFA detection method based on metal nanocluster-labeled antibodies, which can be used for detecting ZEN and other small molecule compounds. Compared with a traditional colloidal gold LFA method, the method has improved speed and sensitivity for detecting small molecules.

The invention provides a flaky gold nanoparticle aggregate as well as a preparation method and application thereof, the preparation method comprises the following steps: step 10, adding a hydrogen peroxide solution and a sodium borohydride solution into a sodium citrate solution, centrifuging to remove supernate after reaction, and redissolving with deionized water to obtain a seed solution; adding the seed solution into the growth solution, reacting for a period of time, then adding a silver nitrate solution, reacting, and centrifuging to remove supernate; re-dissolving with deionized water to obtain a silver nanosheet solution; and step 20, adding the silver nanosheet solution and the monovalent gold solution into the stabilizer solution, and centrifuging to remove supernate after reaction to obtain the flaky gold nanoparticle aggregate. According to the flaky gold nanoparticle aggregate and the preparation method and application thereof, the sensitivity of a lateral flow immunoassay test strip can be enhanced, the preparation process is simple, and no additional operation is added when the lateral flow immunoassay test strip is used for testing.

A lateral flow assay (LFA) device (100) for detecting biomarkers for intrapartum and neonatal complications and method thereof is provided. The LFA device (100) is equipped with a lateral flow immunoassay assembly (102) that integrates a sample pad (104), conjugate release matrix (106) pre-impregnated with bio-functionalized gold nanoparticles (AuNPs) and latex nanoparticles conjugated with capture antibodies specific to biomarkers, a chromatography membrane (108) with one or more test lines (112, 114 and 116) and a control line (118) for analyte detection, and an absorbent pad (110) for excess sample capture. The LFA device (100) enables non-invasive sample analysis and delivers visual results within 15 minutes making it highly suitable for use in time-sensitive clinical scenarios such as suspected P-PROM, neonatal sepsis, or intra-amniotic inflammation.

The invention relates to the technical field of helicobacter pylori detection, in particular to a method and a kit for dual-mode lateral flow immunodetection of helicobacter pylori. According to the technical scheme, the kit comprises a nano-label probe, an immunochromatography test strip and a running buffer solution. According to the invention, the core-shell structure magnetic-fluorescent nanoprobe is constructed, and a direct detection mode and a magnetic enrichment detection mode are innovatively integrated, so that ultra-sensitive and high-specificity detection of helicobacter pylori is successfully realized; on one hand, the detection sensitivity is improved by 50-500 times compared with that of a traditional method, and the lowest detection limit reaches 10 cells / mL; on the other hand, due to the dual-mode design, different sample types from clean to complex (such as excrement) can be flexibly handled, detection can be completed within 15-30 minutes, meanwhile, strong matrix interference resistance and excellent operation convenience are achieved, and the efficiency and accuracy of helicobacter pylori screening and diagnosis are guaranteed.

The embodiment of the invention discloses an electrochemical lateral flow immunoassay test strip as well as a preparation method and application thereof. The invention provides a novel electrochemical lateral flow immunoassay (eLFIA) test strip with a built-in electrode, the test strip is integrated with a portable potential analyzer, a portable sensing device based on an electric signal output by a smart phone is constructed, the portable sensing device is used for detecting cardiac troponin I (cTnI) in human serum, and the whole detection process can be completed within 10 minutes. Specifically, a screen printing technology is adopted, an electrode material is directly printed on an NC film, and preparation of the eLFIA test strip is achieved. The eLFIA test strip prepared by the method shows higher sensitivity and good reproducibility, and has been successfully applied to detection of cTnI in an actual human serum sample. Compared with a commercial ELISA kit, the eLFIA test strip not only has considerable detection limit, but also has the advantages of being simpler and more convenient to operate and higher in detection speed, and shows huge application potential in the field of on-site rapid detection.

A lateral flow immunoassay device includes a porous test strip, a sampling channel having an inlet at a first end to receive a biomarker and an outlet at a second end, opposite the first end, the outlet communicating with the test strip. The sampling channel has an air vent opening at or adjacent the second end thereof, and a diluent reservoir having an outlet communicating with the test strip. The outlet of the sampling channel and the outlet of the diluent reservoir are sealed by a common removable seal to prevent communication between the sampling channel and the test strip, and the diluent reservoir and the test strip, until the seal is removed.

A reflectometer and method for use in measuring the amount of analyte in a sample are provided. The reflectometer can include multiple readheads for obtaining reflectance information from discrete areas on a lateral flow test strip. The reflectance information is processed to obtain digital values ​​for the reflectometer. The discrete areas can include a test zone and at least two control zones, each having a different amount of detection moiety-binding substance. The digital values ​​for each control zone can be used to establish a high-end standard and a unique low-end standard. Extrapolating the digital values ​​for the test zones to the standards provides an accurate quantitative value of the analyte in the sample.

The present application aims to provide an electrochemical lateral flow immunoassay method and a sensor used therefor, which can achieve a short reaction time, excellent sensitivity, high-precision quantitative measurement and electrical detection by controlling the flow of a sample solution. The technical solution is that an electrode portion 5, a conductive portion 7 for conducting current from the electrode portion 5, and a connecting portion 12 connected to an electrical detector 4 for detecting the current value are arranged on a support body 2 made of a resin sheet; and a plurality of pad types 8, 10, 11, 13, 15, 16 are arranged in a partially laminated manner on the support body 2, so that the sample solution flows through the plurality of pad types, and the flow is controlled at the position of the electrode portion 5 to perform electrochemical detection. Then, the flow is controlled by a flow rate control pad 15, a flow path portion fiber pad 10, or flow rate control protrusions 27a, 27b, 27c.

The present invention relates to a method for detecting a target gene and a gene mutation using isothermal single reaction and lateral flow immunoassay. The detection method according to the present invention enables sequence-specific nucleic acid detection and mutation detection at the level of single nucleotide polymorphism (SNP) discrimination, thereby providing a method capable of sensitively distinguishing mutations within a short time. In particular, since sequence-specific nucleic acid detection and single nucleotide polymorphism (SNP) discrimination can be visually confirmed directly through the LFA system, the method has high utility for on-site diagnosis.

To provide a novel assay and / or platform for measuring functional C1-INH involved in HAE disease pathology.SOLUTION: A device and a lateral flow assay (LFA) method performed using such device is a device for detecting and / or quantifying functional C1-esterase inhibitor (fC1-INH), including: (i) a conjugate pad comprising a first zone and a second zone in which a first agent and a second agent are immobilized, respectively; and (ii) a membrane in communication with the conjugate pad, in which the membrane includes a third zone in which a third agent is immobilized.SELECTED DRAWING: Figure 4

The invention provides a cascade amplification method based on Zn < 2 + > doped WOX nano-enzyme and application of the cascade amplification method in clostridium difficile toxin B detection, and relates to the technical field of lateral flow immunoassay. The cascade amplification method comprises the following steps that firstly, Zn / WOX at Au with reductase-like activity is subjected to immunochromatography to generate an initial colorimetric signal; and Zn / WOX (at) Au (at) Pt with peroxidase-like activity is formed through in-situ light-induced deposition of Pt to amplify a colorimetric signal, and then enzymatic color development is performed, so that the technical problem that clostridium difficile toxin B is difficult to quickly and highly sensitively detect in the prior art is solved, the colorimetric signal is remarkably enhanced while the simplicity and convenience of LFIA operation are maintained, and the detection sensitivity of clostridium difficile toxin B is improved. The technical effect of high-sensitivity colorimetric LFIA detection is realized.

The application discloses a chemiluminescence lateral flow immunoassay method and application, and belongs to the technical field of detection. Paraffin liquid is coated on T lines and C lines of a nitrocellulose membrane, and the volume of the paraffin liquid drop is controlled, so that the transparency of the nitrocellulose membrane can be adjusted in the range of 12.47% to 78.53%. The chemiluminescence lateral flow immunoassay method and application based on the paraffin liquid modified nitrocellulose membrane can be applied to detection of a typical acute myocardial infarction biomarker, i.e. cardiac troponin I (cTnI), and the lower limit of detection can reach 0.063 ng / mL, and the method has high sensitivity and reliability. Moreover, the method can effectively distinguish clinical samples with cTnI concentrations in the range of 1 to 5.1 ng / mL, and has high diagnostic accuracy.

This invention discloses a non-competitive lateral flow immunoassay method based on dual-signal mode of AIE nanoparticles, belonging to the field of agricultural product detection technology. This invention uses an organic solvent swelling method to prepare PSNPs@AIE. 650 Hydrophilic AIE nanoparticles were used to label anti-immune complex peptides (AICPs) with streptavidin-biotin system via an active esterification method to obtain PSNPs@AIE. 650 @AICP immunorecognition probe; Construct a dual-signal non-competitive lateral flow immunochromatographic system for the detection of thiamethoxam residues in agricultural products. This invention achieves colorimetric + fluorescence dual-signal readout, with a colorimetric visualization detection limit of 1.0 ng / mL and a quantitative detection limit of 0.45 ng / mL; a fluorescence visualization detection limit of 0.25 ng / mL and a quantitative detection limit of 0.23 ng / mL. It has the advantages of high sensitivity, strong specificity, rapid portability, and resistance to matrix interference, effectively solving the problem of insufficient sensitivity in traditional colloidal gold lateral flow immunoassay, and is suitable for rapid on-site detection of small molecule pesticide residues in agricultural products.

The present invention provides a device and method based on lateral flow immunoassay for CC16 semi-quantification in serum sample. A novel membrane based semi quantitative detection of physiological / pathological levels of CC16 in the serum has been provided. The device of the present invention provides affordable and easy to use strip-based screening approach for early detection of silicosis using CC16 as a biomarker.

Lateral flow assay devices, systems, and methods described herein separate components of a fluid sample, including small volume, undiluted, unprocessed samples. In one aspect, components are retained in a separation membrane that is spatially above and in fluid communication with a conjugate pad of a lateral flow assay. Devices, systems, and methods described herein can retain particles from a fluid sample that obstruct flow of the fluid sample through the conjugate pad to a detection zone and / or interfere with detection of an analyte of interest in the detection zone.

The application discloses a kind of based on CMOS lens-free imaging lateral flow immunoassay chip and preparation method, device and use method.The chip includes: printable immunochromatography membrane, lens-free image sensor, chip expansion board, first chip electrode, chip handheld area, light transmission flat protection film, sample pad, binding pad and water absorption pad.The device includes lateral flow immunoassay chip and lateral flow immunoassay instrument;Lateral flow immunoassay instrument includes multifunctional shell, LED unit, microprocessor unit and power supply.The device is small in size, high throughput, high sensitivity, realizes integrated device to the instant detection of multiple target detection objects, improves detection efficiency.

This invention relates to the field of image sequence processing technology and discloses an ultra-fast quantitative detection method for lateral tomographic immunoassay image sequences. The specific steps are as follows: Step 1: Continuously acquire LFIA fluorescence images at equal time intervals to form an image sequence; Step 2: Preprocess the continuously acquired LFIA fluorescence images to obtain a preprocessed image sequence; Step 3: Use a convolutional neural network (CNN) to independently extract spatial features from each frame of the preprocessed image to obtain a spatial feature sequence; Step 4: Use a bidirectional long short-term memory network to simultaneously process the spatial feature sequence forward and backward, and then aggregate the data from the forward and backward processing to obtain the time-dependent features of the image sequence, and aggregate all the time-dependent features into a time-dependent feature sequence. This invention demonstrates significant advantages and good practical application value in terms of classification accuracy, structural efficiency, and engineering deployability.

A lateral flow immunometer 10 includes a sample accommodation portion 20 configured to accommodate a sample; a SERS active particle configured to bind to cytokeratin-18 (K18) in the sample, and a test portion 40 configured to immobilize the SERS active particle that has bound to K18.

A nanobody-based point-of-care lateral flow immunoassay (LFA) for the rapid, cost-effective detection of SARS-CoV-2 and MERS-CoV proteins in biological samples is disclosed. The assay described herein uses nanobody-based binding agents that selectively capture and detect viral antigens, such as spike (S) proteins and receptor-binding domains (RBDs), with high sensitivity and specificity. The LFA utilizes a colorimetric readout visible to the naked eye, eliminating the need for specialized equipment. The assay supports single and multiplex detection formats, enabling simultaneous analysis of multiple viral analytes. The LFAs are stable under standard storage conditions and provide a practical solution for decentralized and scalable testing.

A lateral flow immunoassay device and associated reagents for confirming the presence of one or more analytes in a liquid sample. The detection zone of the membrane supporting the lateral flow contains an immobilized, dried capture reagent comprising a first antibody conjugated to a first binding partner conjugated to a second binding partner conjugated to a particle. This disclosure generally relates to a lateral flow immunoassay device and associated reagents for confirming the presence of one or more analytes in a liquid sample.

The invention relates to the technical field of lateral flow immunodetection, and discloses a multi-mode lateral flow immunodetection system based on a PdPtRu nanoprobe, and the multi-mode lateral flow immunodetection system comprises lateral flow immunodetection test paper, a color developing solution and a signal acquisition module; the lateral flow immune test paper comprises a PdPtRu nanoprobe, a nitrocellulose membrane, a sample pad and a water absorption pad, the acquisition module is used for acquiring absorbance and temperature signals of the detected lateral flow immune test paper. The three-metal palladium-platinum-ruthenium (PdPtRu) nano-probe with an adjustable structure is designed, the nano-probe has a branched metal structure with surface bulges and internal nano-pores, and the geometric structure can generate local electromagnetic field concentration on the surface of the nano-probe, so that photo-thermal and catalytic response is amplified; colorimetric, photo-thermal and catalytic signal multi-mode output in lateral flow immunochromatography (LFIA) is realized, and the detection sensitivity, the signal stability and the applicability are further improved.

This invention discloses a lateral immunoassay kit and method for detecting aflatoxin B1, belonging to the field of lateral immunoassay technology. The kit includes a nanobody-biotin / streptavidin@quantum dot probe and a lateral immunoassay test strip. The lateral immunoassay test strip has a T-line and a C-line on its nitrocellulose membrane. The T-line is coated with AFB1-BSA antigen, and the C-line is coated with biotin-labeled bovine serum albumin. The directional coupling strategy of this invention overcomes the defect of reduced nanobody activity caused by random modification, greatly preserving the activity of the nanobody. Utilizing smartphone photography for grayscale reading, it features rapid, accurate, stable, and instrument-free on-site analysis, possessing broad application potential and significant market prospects.