48 results about "Lateral flow immunoassay" patented technology

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

Methods, apparatus, systems and articles of manufacture to make and / or process diagnostic tests are disclosed. An example test device, such as a lateral flow immunoassay device, includes a conjugate pad including conjugates for a target analyte; and a test grid including a plurality of zones in a two-dimensional grid, the test grid including: a first test zone in the plurality of zones including at least one of first immobilized antibodies or first immobilized antigens to attach to the target analyte labeled with a first conjugate of the conjugates ; and a second test zone in the plurality of zones including at least one of second immobilized antibodies or second immobilized antigens to attach to the target analyte labeled with a second conjugate of the conjugates.

Lateral flow immunoassay systems include test strips having unique geometries to enhance fluid dynamics, including but not limited to: (1) conjugate pads having sinusoidal, meandering, and nozzle-diffuser patterns; and (2) nitrocellulose membrane pads having constriction zones corresponding to the location of T-lines and / or C-lines. The systems can further include a cartridge with a two-step rotation mechanism for contamination-free sample delivery to the sample pad of a test strip, and an AI-based detection system for quantifying the concentration of a detected analyte based on colorimetric signal intensities. The system exhibits improved analyte sensitivity over prior systems, reduces traditional assay time, eliminates subjective interpretation of colorimetric signal intensities, and quantifies analyte concentration levels.

The disclosure relates to a point-of-care test device for diagnosis and detection of bacterial infections and hidden septic processes in samples of bodily fluids of premature and newborns comprising a quantitative or semi-quantitative lateral flow immunoassay unit adapted for parallel detection of proteins of the S100 family and for several detection of increased amounts of S100A12 and calprotectin in blood, serum, plasma, and saliva.

The present invention relates to lateral flow immunoassay devices, including test strips, having a top layer, a bottom layer, and a middle layer, wherein the middle layer defines a fluid flow path and the top and bottom layers provide hydrophilic surfaces in contact with the middle layer. The present invention also relates to methods of detecting analytes of interest using said devices. The present invention also relates to methods of preparing immunoassay devices involving laminating a bottom layer, a middle layer, and a top layer together, wherein the middle layer defines a fluid flow path and the top and bottom layers provide hydrophilic surfaces in contact with the middle layer.

The present invention relates to multiplex lateral flow immunochromatographic diagnostic devices configured to detect immune activity biomarkers in biological samples. The device is configured to detect granulysin as a biomarker indicative of cytotoxic immune effector activity and at least one secondary biomarker indicative of tissue-associated immune injury. In certain embodiments, the device comprises a sample pad, a conjugate pad containing labeled detection antibodies, a porous membrane comprising spatially distinct capture zones corresponding to granulysin and the secondary biomarker, a control zone, and an absorbent pad configured to facilitate capillary flow. Detection of granulysin and the secondary biomarker within a single multiplex device enables determination of immune activation state and tissue-associated immune context from a single biological sample. The device is suitable for rapid diagnostic use, including point-of-care and laboratory environments, using whole blood, plasma, serum, or related biological fluids.

The invention provides methods and systems for detecting a biomarker in a synovial fluid wherein the system also includes a control to ensure that the test sample is indeed synovial fluid. The biomarkers and the control for synovial fluid can be identified using proteomic methods, including but not limited to antibody based methods, such as an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a lateral flow immunoassay.

The present disclosure is directed to methods for fabricating cellulose-based microneedles with exceptional wicking properties which can be used for rapid extraction of dermal interstitial fluid (ISF). Microneedle arrays are fabricated through a combination of compression molding and laser etching, utilizing microcrystalline cellulose, a biocompatible material with excellent wicking properties. These microneedles are mechanically robust and can autonomously extract fluid from the skin without the need for external instruments. As such, they can be directly integrated with paper-based lateral flow immunoassays for point-of-care disease detection.

The invention relates to the technical field of food safety detection, and discloses a multi-modal detection method for pathogenic bacteria based on a nano composite material and a neural network, and the method comprises the following steps: preparing the nano composite material based on the metal chelating property of cuttlefish ink source nano particles and an interface regulation and control technology; constructing a dual-mode immunosensing detection platform based on the nano composite material and a lateral flow immunoassay detection platform; the method comprises the following steps: primarily detecting pathogenic bacteria based on a dual-mode immunosensing detection platform to obtain a dual-mode detection signal; extracting a multi-mode signal feature of the dual-mode detection signal based on a fusion architecture of a multi-layer sensor and a radial basis function; and analyzing the multi-modal signal characteristics based on a neural network algorithm, and outputting a detection result and a prediction result of the pathogenic bacteria. Nano-composite engineering, multi-modal signal transduction and machine learning are organically combined, the bottleneck of a traditional detection technology is broken through, and bimodal detection of rapid and accurate detection and risk assessment of the food-borne pathogenic bacteria is achieved.

A device for detecting and / or quantifying functional C1-esterase inhibitor (fC1-INH), the device comprising: (i) a conjugate pad comprising a first zone and a second zone, on which a first agent and a second agent are immobilized, respectively, and (ii) a membrane, which is in communication with the conjugate pad, wherein the membrane comprises a third zone, on which a third agent is immobilized. The conjugate pad may further comprise a fourth zone for placing a biological sample, which flows through the device in the order of the first zone, the second zone, and the third zone.

A disease detection system including a platform or cartridge to perform testing using a rapid lateral flow chromatographic immunoassay (LFIA) test intended for the qualitative detection of the disease in a sample, such as collected sputum, disrupted by a digestive enzyme and a detergent to release its content and digest its proteins and other polymeric molecules. A method of the present invention includes treating a sample with a digestive enzyme and a detergent to disrupt the collected sample releasing content and digesting proteins of the sample to form a lysed-digested-extracted sample, applying the lysed-digested-extracted sample to a platform or cartridge, performing a rapid lateral flow chromatographic immunoassay (LFIA) test of the lysed-digested-extracted sample using the platform or cartridge and detecting presence or absence of an analyte of interest from the tested lysed-digested-extracted sample.

An embodiment relates to a reagent buffer comprising a zwitterionic sulfonic solution, a non-ionic surfactant solution, and a buffer solution, wherein the reagent buffer has a pH range of about 7.0 to about 8.0 and a circular dichroism with an absorbance peak more than 0 at 190 nm and 225 nm. In an embodiment, the reagent buffer is configured to be a reagent buffer for a lateral flow immunoassay for detection of COVID-19.

Lateral flow immunoassay systems include test strips having unique geometries to enhance fluid dynamics, including but not limited to: (1) conjugate pads having sinusoidal, meandering, and nozzle-diffuser patterns; and (2) nitrocellulose membrane pads having constriction zones corresponding to the location of T-lines and / or C-lines. The systems can further include a cartridge with a two-step rotation mechanism for contamination-free sample delivery to the sample pad of a test strip, and an AI-based detection system for quantifying the concentration of a detected analyte based on colorimetric signal intensities. The system exhibits improved analyte sensitivity over prior systems, reduces traditional assay time, eliminates subjective interpretation of colorimetric signal intensities, and quantifies analyte concentration levels.

The application relates to the technical field of food safety detection, and discloses a pathogenic bacteria multi-modal detection method based on a nano-composite material and a neural network, which comprises the following steps: preparing a nano-composite material based on the metal chelating property and interface regulation technology of cuttlefish ink source nanoparticles; constructing a dual-mode immunosensing detection platform based on the nano-composite material and a lateral flow immunoassay detection platform; preliminarily detecting pathogenic bacteria based on the dual-mode immunosensing detection platform to obtain dual-mode detection signals; extracting multi-modal signal characteristics of the dual-mode detection signals based on a fusion architecture of a multilayer perceptron and a radial basis function; and analyzing the multi-modal signal characteristics based on a neural network algorithm and outputting detection results and prediction results of the pathogenic bacteria. The application combines nano-composite engineering, multi-modal signal transduction and machine learning organically, breaks through the bottleneck of traditional detection technology, and realizes dual-mode detection of rapid and accurate detection and risk assessment of foodborne pathogenic bacteria.

The application discloses a fluorescent lateral flow immunoassay method and preparation and application of a test strip thereof, and the AuNCs-LFA test strip comprises a sample pad, a gold label pad, an NC membrane, an absorption pad and a PVC bottom plate; wherein the NC membrane comprises a signal probe AuNCs-Ab, an antigen ZEN-BSA and a goat anti-mouse lgG. The application provides a fluorescent LFA detection method based on metal nanocluster-labeled antibodies, which can be used for detecting ZEN and other small molecule compounds. Compared with a traditional colloidal gold LFA method, the method has improved speed and sensitivity for detecting small molecules.

The invention provides a flaky gold nanoparticle aggregate as well as a preparation method and application thereof, the preparation method comprises the following steps: step 10, adding a hydrogen peroxide solution and a sodium borohydride solution into a sodium citrate solution, centrifuging to remove supernate after reaction, and redissolving with deionized water to obtain a seed solution; adding the seed solution into the growth solution, reacting for a period of time, then adding a silver nitrate solution, reacting, and centrifuging to remove supernate; re-dissolving with deionized water to obtain a silver nanosheet solution; and step 20, adding the silver nanosheet solution and the monovalent gold solution into the stabilizer solution, and centrifuging to remove supernate after reaction to obtain the flaky gold nanoparticle aggregate. According to the flaky gold nanoparticle aggregate and the preparation method and application thereof, the sensitivity of a lateral flow immunoassay test strip can be enhanced, the preparation process is simple, and no additional operation is added when the lateral flow immunoassay test strip is used for testing.

A lateral flow assay (LFA) device (100) for detecting biomarkers for intrapartum and neonatal complications and method thereof is provided. The LFA device (100) is equipped with a lateral flow immunoassay assembly (102) that integrates a sample pad (104), conjugate release matrix (106) pre-impregnated with bio-functionalized gold nanoparticles (AuNPs) and latex nanoparticles conjugated with capture antibodies specific to biomarkers, a chromatography membrane (108) with one or more test lines (112, 114 and 116) and a control line (118) for analyte detection, and an absorbent pad (110) for excess sample capture. The LFA device (100) enables non-invasive sample analysis and delivers visual results within 15 minutes making it highly suitable for use in time-sensitive clinical scenarios such as suspected P-PROM, neonatal sepsis, or intra-amniotic inflammation.

The invention relates to the technical field of helicobacter pylori detection, in particular to a method and a kit for dual-mode lateral flow immunodetection of helicobacter pylori. According to the technical scheme, the kit comprises a nano-label probe, an immunochromatography test strip and a running buffer solution. According to the invention, the core-shell structure magnetic-fluorescent nanoprobe is constructed, and a direct detection mode and a magnetic enrichment detection mode are innovatively integrated, so that ultra-sensitive and high-specificity detection of helicobacter pylori is successfully realized; on one hand, the detection sensitivity is improved by 50-500 times compared with that of a traditional method, and the lowest detection limit reaches 10 cells / mL; on the other hand, due to the dual-mode design, different sample types from clean to complex (such as excrement) can be flexibly handled, detection can be completed within 15-30 minutes, meanwhile, strong matrix interference resistance and excellent operation convenience are achieved, and the efficiency and accuracy of helicobacter pylori screening and diagnosis are guaranteed.

The embodiment of the invention discloses an electrochemical lateral flow immunoassay test strip as well as a preparation method and application thereof. The invention provides a novel electrochemical lateral flow immunoassay (eLFIA) test strip with a built-in electrode, the test strip is integrated with a portable potential analyzer, a portable sensing device based on an electric signal output by a smart phone is constructed, the portable sensing device is used for detecting cardiac troponin I (cTnI) in human serum, and the whole detection process can be completed within 10 minutes. Specifically, a screen printing technology is adopted, an electrode material is directly printed on an NC film, and preparation of the eLFIA test strip is achieved. The eLFIA test strip prepared by the method shows higher sensitivity and good reproducibility, and has been successfully applied to detection of cTnI in an actual human serum sample. Compared with a commercial ELISA kit, the eLFIA test strip not only has considerable detection limit, but also has the advantages of being simpler and more convenient to operate and higher in detection speed, and shows huge application potential in the field of on-site rapid detection.

A lateral flow immunoassay device includes a porous test strip, a sampling channel having an inlet at a first end to receive a biomarker and an outlet at a second end, opposite the first end, the outlet communicating with the test strip. The sampling channel has an air vent opening at or adjacent the second end thereof, and a diluent reservoir having an outlet communicating with the test strip. The outlet of the sampling channel and the outlet of the diluent reservoir are sealed by a common removable seal to prevent communication between the sampling channel and the test strip, and the diluent reservoir and the test strip, until the seal is removed.

An apparatus for detecting and / or quantifying functional C1-esterase inhibitor (fC1-INH), the apparatus comprising: (i) a conjugate pad including a first zone and a second zone, on which a first reagent and a second reagent are respectively immobilized; and (ii) a membrane in communication with the conjugate pad, wherein the membrane includes a third zone, on which a third reagent is immobilized. The conjugate pad may further include a fourth zone for placing a biological sample, and the biological sample flows through the apparatus in the order of the first zone, the second zone, and the third zone.

According to the lateral flow immunoassay test paper quantitative detection system and method based on deep learning, an innovative differential attention mechanism is integrated, key space-time dynamic characteristics of an LFA test strip within 3 minutes can be efficiently extracted and analyzed, high-precision quantitative detection can be completed within extremely short time, the diagnosis time is greatly shortened, and the detection efficiency is improved. Meanwhile, the accuracy of quantitative analysis and the objectivity of a detection result are remarkably improved; a unique time embedding module is built in a differential attention mechanism, and the module enhances the sensitivity of the model to the sequence change of an image sequence and supports built-in interpretability analysis. The requirements of instant detection on equipment miniaturization, low cost, convenient operation and highly reliable result are met.

A reflectometer and method for use in measuring the amount of analyte in a sample are provided. The reflectometer can include multiple readheads for obtaining reflectance information from discrete areas on a lateral flow test strip. The reflectance information is processed to obtain digital values ​​for the reflectometer. The discrete areas can include a test zone and at least two control zones, each having a different amount of detection moiety-binding substance. The digital values ​​for each control zone can be used to establish a high-end standard and a unique low-end standard. Extrapolating the digital values ​​for the test zones to the standards provides an accurate quantitative value of the analyte in the sample.

The present application aims to provide an electrochemical lateral flow immunoassay method and a sensor used therefor, which can achieve a short reaction time, excellent sensitivity, high-precision quantitative measurement and electrical detection by controlling the flow of a sample solution. The technical solution is that an electrode portion 5, a conductive portion 7 for conducting current from the electrode portion 5, and a connecting portion 12 connected to an electrical detector 4 for detecting the current value are arranged on a support body 2 made of a resin sheet; and a plurality of pad types 8, 10, 11, 13, 15, 16 are arranged in a partially laminated manner on the support body 2, so that the sample solution flows through the plurality of pad types, and the flow is controlled at the position of the electrode portion 5 to perform electrochemical detection. Then, the flow is controlled by a flow rate control pad 15, a flow path portion fiber pad 10, or flow rate control protrusions 27a, 27b, 27c.

The present invention relates to a method for detecting a target gene and a gene mutation using isothermal single reaction and lateral flow immunoassay. The detection method according to the present invention enables sequence-specific nucleic acid detection and mutation detection at the level of single nucleotide polymorphism (SNP) discrimination, thereby providing a method capable of sensitively distinguishing mutations within a short time. In particular, since sequence-specific nucleic acid detection and single nucleotide polymorphism (SNP) discrimination can be visually confirmed directly through the LFA system, the method has high utility for on-site diagnosis.

A lateral flow immunoassay for the detection of anti-drug antibodies against a biological drug includes a membrane having a capture area, a sample application area, a flow path from the sample application area to the capture area, and a conjugate area located in the flow path. The conjugate area has said biological drug detectably labeled and the capture area has said biological drug immobilized thereto.

To provide a novel assay and / or platform for measuring functional C1-INH involved in HAE disease pathology.SOLUTION: A device and a lateral flow assay (LFA) method performed using such device is a device for detecting and / or quantifying functional C1-esterase inhibitor (fC1-INH), including: (i) a conjugate pad comprising a first zone and a second zone in which a first agent and a second agent are immobilized, respectively; and (ii) a membrane in communication with the conjugate pad, in which the membrane includes a third zone in which a third agent is immobilized.SELECTED DRAWING: Figure 4

The invention provides a cascade amplification method based on Zn < 2 + > doped WOX nano-enzyme and application of the cascade amplification method in clostridium difficile toxin B detection, and relates to the technical field of lateral flow immunoassay. The cascade amplification method comprises the following steps that firstly, Zn / WOX at Au with reductase-like activity is subjected to immunochromatography to generate an initial colorimetric signal; and Zn / WOX (at) Au (at) Pt with peroxidase-like activity is formed through in-situ light-induced deposition of Pt to amplify a colorimetric signal, and then enzymatic color development is performed, so that the technical problem that clostridium difficile toxin B is difficult to quickly and highly sensitively detect in the prior art is solved, the colorimetric signal is remarkably enhanced while the simplicity and convenience of LFIA operation are maintained, and the detection sensitivity of clostridium difficile toxin B is improved. The technical effect of high-sensitivity colorimetric LFIA detection is realized.

The application discloses a chemiluminescence lateral flow immunoassay method and application, and belongs to the technical field of detection. Paraffin liquid is coated on T lines and C lines of a nitrocellulose membrane, and the volume of the paraffin liquid drop is controlled, so that the transparency of the nitrocellulose membrane can be adjusted in the range of 12.47% to 78.53%. The chemiluminescence lateral flow immunoassay method and application based on the paraffin liquid modified nitrocellulose membrane can be applied to detection of a typical acute myocardial infarction biomarker, i.e. cardiac troponin I (cTnI), and the lower limit of detection can reach 0.063 ng / mL, and the method has high sensitivity and reliability. Moreover, the method can effectively distinguish clinical samples with cTnI concentrations in the range of 1 to 5.1 ng / mL, and has high diagnostic accuracy.