19 results about "Nanoprobe" patented technology

The application discloses a kind of near-infrared benzothiadiazole dye sensitized rare earth upconversion luminescence nano-probes and preparation method thereof;Probe is composed of near-infrared benzothiadiazole dye and upconversion nanoparticle, benzothiadiazole dye is compounded with ligand-free lanthanide ion doped upconversion nanoparticle, and upconversion luminescence is enhanced.This application preparation method is simple, and it does not involve complex core-shell structure and energy migration process, benzothiadiazole dye is directly compounded with ligand-free rare earth nanoparticle, and upconversion luminescence nano-probe is formed, under the irradiation of 808nm laser, the emission spectrum of benzothiadiazole dye and the absorption spectrum of sensitizer ion in upconversion nanoparticle overlap, which can effectively enhance the upconversion luminescence intensity of nano-probe, and keep good light stability, keep more than 90% of initial luminescence intensity under the irradiation of 120 minutes excitation light.

The application discloses a pure titanium nanometer scale oxide layer nanometer probe precise detection method, which comprises the following steps: S1, preparing a titanium sample to be measured, and pretreating the titanium sample to be measured to make the surface of the titanium sample to be measured smooth; S2, selecting multiple test points on the surface of the titanium sample to be measured and marking the test points, using a pressure head of a nano indentation instrument to make an indentation on the marked test points, and obtaining surface indentation data; S3, selecting multiple test points on the cross section of the titanium sample to be measured and marking the test points, using the pressure head of the nano indentation instrument to make an indentation on the marked test points, and obtaining cross section indentation data; S4, summing up the maximum contact depth in the surface indentation data of each test point and taking an average value, and marking the average value as a; summing up the maximum contact depth in the cross section indentation data of each test point and taking an average value, and marking the average value as b; and taking a difference between a and b to obtain an influence value of the oxide layer on the titanium surface. The application can simply and quickly evaluate the influence of the oxide layer on the titanium surface by using the nano indentation method.

A method for preparing bimodal nanocapsules includes: preparing a solution of mesoporous silica and deionized water, adding chloroauric acid solution and stirring, then adding sodium borohydride solution under ice bath stirring conditions to obtain gold nanoparticle-modified mesoporous silica; preparing a solvent of ultrapure water and gold nanoparticle-modified mesoporous silica, then sequentially adding a fluorescence quencher, a fluorescent molecule, and 4-mercaptophenylboronic acid to react separately to obtain bimolecularly modified nanoprobes; and mixing an adenosine-5′-triphosphate nucleic acid aptamer solution with the nanoprobes using ultrapure water as a solvent to obtain nucleic acid aptamer-encapsulated bimodal nanocapsules. This invention provides a simple, mild, environmentally friendly, and low-cost preparation method. The bimodal nanocapsules prepared by this invention can simultaneously monitor the levels of hydrogen peroxide and adenosine-5′-triphosphate (ATP) in cells using fluorescence and surface-enhanced Raman scattering (SERS) techniques, which can be used for early disease monitoring.

This application provides a nanoprobe-based electrophysiological signal and biochemical biomarker detection system, comprising: a patch-clamp amplifier; a working electrode electrically connected to a signal acquisition module, the surface of which, except for the tip, is covered by an insulating protective layer, forming a GΩ-level impedance seal after implantation into the cell; a counter electrode connected to a BATH port, with its active end placed in the matrix environment of the target cell, forming a current loop; a reference electrode connected to a REF port, with its active end placed in the matrix environment of the target cell to provide a potential reference point; the patch-clamp amplifier is configured to operate in voltage-clamp mode, with the REF port connected to the reference electrode as a voltage sensing terminal, and the BATH port connected to the counter electrode as a current output terminal, simultaneously acquiring the electrophysiological signal of the target cell and the current signal corresponding to the concentration of the target biochemical biomarker. This application provides a powerful analytical tool for a deeper understanding of the electrochemical signal coupling mechanism of neurons.

This invention discloses a nanoprobe for detecting thimerophos and an immunochromatographic method for detecting thimerophos. The nanoprobe for detecting thimerophos comprises a labeled antibody complex. This invention utilizes the reaction of dopamine with the thiol group of 4-MBA under alkaline conditions to polymerize a large amount of 4-MBA, providing a stronger SERS signal. Dopamine itself possesses a strong photothermal effect, enhancing the detection signal and thus increasing sensitivity. By adding a TEOS-coated silica shell, the coupling rate with the target antibody is increased, improving the inhibition rate of the immunochromatographic strip. The detection limit for thimerophos using micro Raman spectroscopy is 20.42 ng / mL, and the detection limit using an 808 near-infrared spectroscopy instrument is 23.53 ng / mL. The detection limit of the traditional colloidal gold immunochromatographic method is 82.49 ng / mL. Compared with the traditional colloidal gold immunochromatographic method, the multimodal high-sensitivity immunochromatographic technique using core-shell nanoprobes improves the sensitivity of thimerophos detection by approximately four times.

The application provides a COB display module full-dimension detection system and method based on nanoprobes and quantum dot spectrum fusion, and belongs to the technical field of COB display module detection; the system comprises a hardware layer and a software layer, wherein the hardware layer comprises a motion control module, a detection module, an environment control module and an edge computing unit; the software layer comprises a COB special AI algorithm library for identifying continuous tin defects on a COB display module, analyzing the brightness and chrominance of the COB display module and predicting the service life of the COB display module, an edge computing and data management architecture and an HMI human-computer interaction interface; the application integrates an atomic force microscope probe, quantum dot spectrum imaging, a Transformer defect recognition and light-heat-electricity coupling analysis, is suitable for novel display technologies such as Micro LED and OLED, and can realize full-dimension high-precision detection of chip-level electrical performance, micron-level continuous tin defects, multi-view optical performance and thermal management.

The application discloses a kind of nanocluster structures for improving light absorption efficiency, to provide a kind of nanocluster structure with stable high absorption efficiency under near-infrared light irradiation.The present application is based on the random agglomeration of nanochip cap structure monomer that only grows connecting molecule on cap surface, weaken the dependence of nanocluster structure on incident light polarization state;By wrapping the connecting surface into the cluster structure, reduce the contact between the connecting surface and the outside world, limit the number of particles in the cluster structure, limit the absorption spectrum widening of cluster structure by using the plasmonic coupling between limited particles, improve the light absorption efficiency of nanocluster structure.Based on the nanoprobes of the nanocluster structure have wide application prospect in photothermal therapy, biosensing, drug delivery and the like.

The application discloses a carbon-coated zirconium oxide composite material, a colorimetric / photothermal dual-mode signal nano probe and application thereof in rapid quantitative detection of chikungunya virus. The colorimetric / photothermal dual-mode signal nano probe is formed by coupling a carbon-coated zirconium oxide composite nano material obtained by calcining mesoporous UiO-66 in an inert atmosphere with a chikungunya virus E2 protein monoclonal antibody. In a specific application, a chromatography test strip is constructed based on the colorimetric / photothermal dual-mode signal nano probe. The chromatography test strip comprises a nitrocellulose membrane provided with a T line and a C line. During detection, a sample and the nano probe form an immune complex and generate a colorimetric signal visible to the naked eye at the T line, and meanwhile, under the irradiation of 808 nm near-infrared laser, a temperature rise Delta T related to the concentration of a target object is generated at the T line, so that on-site rapid screening and photothermal quantitative review are realized. The chromatography test strip has both simple operation and reliable quantification, and has stronger anti-interference ability in a complex biological matrix, and is suitable for instant detection of chikungunya virus.

The application discloses a self-assembled aggregation-induced emission nanoprobes for targeted therapy and real-time imaging of non-small cell lung cancer and application, and belongs to the technical field of biological medicine. The self-assembled aggregation-induced emission nanoprobes AIEnps are used for targeting c-Met, and the AIEnps have a dual function: one is to realize real-time imaging through fluorescence tracing, and the other is to realize targeted therapy by inhibiting c-Met. The design based on AIE realizes tumor-specific accumulation and fluorescence tracing, overcomes the limitations of poor tumor permeability and non-specific distribution in traditional therapy, and the AIEnps are a multifunctional NSCLC treatment platform, integrating targeted therapy and real-time imaging, and have a very broad application prospect.

This application provides a cantilever beam-nanoprobe fabrication device and method based on electrolysis and microassembly. The device includes an air-floating optical platform, an in-situ electrochemical electrolysis system, and a microassembly system. The microassembly system is used to clamp and assemble the cantilever beam structure and metal wire, which are bonded together with conductive adhesive to form a probe preform. The in-situ electrochemical electrolysis system is used to perform in-situ electrochemical electrolysis on the probe preform. By controlling the electrolysis process parameters, a cantilever beam-nanoprobe with precisely controllable structural parameters is prepared. The air-floating optical platform supports the microassembly system and the in-situ electrochemical electrolysis system. This application adopts in-situ electrochemical electrolysis and microassembly, which can significantly reduce equipment and processing costs. The process is simple, easy to operate, and significantly improves processing efficiency and yield, making it suitable for large-scale production and meeting the application needs of high-precision micro-nano detection and characterization in various scenarios.

The application discloses a double-probe tunneling electron beam transmission detection device and an alignment and transmission detection method in the field of nano detection technology, which comprises a bottom plate, a first probe assembly and a second probe assembly are symmetrically arranged on the bottom plate, and a sample conveying mechanism is arranged between the two probe assemblies; the first probe assembly comprises a first nano probe with a first tip; the second probe assembly comprises a second nano probe with a second tip, the second tip is arranged opposite to the first tip, and a tunneling gap is formed between the two tips; a bias power supply is connected between the first nano probe and the second nano probe, and is used for applying a bias between the two probes to generate a tunneling electron beam in the tunneling gap; the device can be used for transmission imaging, cross-section profile measurement and three-dimensional topography reconstruction of nano-scale material structures, and can also be used for measuring tunneling electron characteristics in basic physical research, alignment locking is realized by searching for a maximum value of a tunneling current through XY plane scanning, and the tips of the two probes are accurately aligned in the nano scale.

This invention provides the preparation and application of a two-dimensional photoacoustic nanoprobe, using two-dimensional FeMoO2. v Application in the preparation of photoacoustic imaging reagents for liver diseases; wherein v is 3.0-4.5. The two-dimensional FeMoO of the present invention. v It exhibits specific photoacoustic imaging properties for glutathione, a biotransduction marker in the liver. Two-dimensional FeMoO v Due to its excellent electrical, optical and mechanical properties, the material exhibits higher stability and hepatocyte targeted uptake efficiency in complex biological systems, thereby enabling more accurate in situ imaging.

The invention relates to the technical field of biomedical materials and cancer treatment, in particular to a near-infrared two-region optical diagnosis and treatment functional molecule based on sulfur-sulfur space interaction, a nano probe and application of the near-infrared two-region optical diagnosis and treatment functional molecule and the nano probe. According to the invention, the sulfur-sulfur space interaction is utilized for the first time to construct the NIR-II light diagnosis and treatment functional molecule, through cross-space conjugate extension and conformation locking, the limitation of energy gap law is broken through, long-wavelength emission and high fluorescence quantum yield are realized, and the method has important significance for promoting the development of a precise cancer diagnosis and treatment technology.

The application discloses a magnetic nanometer probe with small size and high magnetic particle imaging performance, and a preparation method and application thereof. In the application, iron chloride is dissolved in a glycol solution, and a uniform transparent solution is formed through magnetic stirring; manganese chloride with different concentrations is added into the above solution, and stirring and dissolution are carried out; sodium acetate is added, and uniform stirring is carried out to form a transparent solution; finally, sodium citrate is dissolved in the above solution, uniform stirring is carried out, and the mixed solution is reacted through a solvothermal method; after the reaction is completed, the reaction product is purified through centrifugation and dialysis; finally, the supernatant is collected and concentrated, and the magnetic nanometer probe is obtained. The magnetic nanometer probe prepared through the method has small size, high sensitive imaging performance and excellent hydrophilic performance, and is expected to be used for the diagnosis of micro lesions.

This invention belongs to the field of biomedical nanomaterials technology, and provides a magnetic resonance / NIR-II fluorescence imaging dual-modal nanoprobe, its preparation method, and its application. The magnetic resonance / NIR-II fluorescence dual-modal imaging nanoprobe of this invention comprises amino- or thiol-modified mesoporous silica nanoparticles, an NIR-II fluorescent dye linked to the mesoporous silica nanoparticles, bovine serum albumin linked to the NIR-II fluorescent dye, and a gadolinium-based magnetic resonance contrast agent linked to the bovine serum albumin. This invention uses bovine serum albumin as a "molecular bridge," utilizing various intermolecular forces to stably and firmly bind the NIR-II fluorescent dye and gadolinium-based magnetic resonance contrast agent to the mesoporous silica nanoparticles, forming a highly stable and biocompatible MRI / NIR-II fluorescence dual-modal imaging nanoprobe. This improves the contrast agent loading rate and stability, and avoids the problem of contrast agent leakage during in vivo circulation.

The application discloses preparation and application of intelligent response nanoprobes based on manganese sulfide, and the nanoprobes are water-soluble and size-controllable HAMnS nanoparticles coated / chelated with hyaluronic acid as a surface functional layer, and near-infrared fluorescent dyes are loaded on the surface of the nanoparticles through electrostatic interaction and coordination / amide coupling to construct pH / ROS double-lock response "OFF-ON" fluorescent photoacoustic magnetic resonance multi-modal nanoprobes. In the physiological neutral and low ROS circulation, the dyes are in a fluorescence quenching state due to the close distance / accumulation / electron transfer with the MnS surface, so that the background is extremely low; when the probes are enriched in the tumor / inflammatory / metastatic lymph node and other lesion microenvironments through EPR effect and HA-mediated CD44 targeting, weak acidity induces MnS degradation and combines with ROS oxidation / ligand replacement to release free dyes or eliminate quenching, so that strong near-infrared fluorescence is lighted up; the change of component density provides a photoacoustic tracking signal, so that intraoperative real-time navigation and accurate boundary judgment from the molecular level to anatomical positioning are realized.

The application discloses a longitudinal resolution near-field infrared spectrum measurement system and method, and relates to the field of spectrum measurement. In the system, the half-reflection half-transmission lens in the interference measurement module transmits infrared light to a nano probe; the nano probe excites the near-field infrared light field formed by local near field to interact with a sample to be measured, so that a backscattering light signal is obtained; the half-reflection half-transmission lens combines the reference light and the backscattering light signal and generates interference to obtain a near-field interference signal; a control module controls a moving mirror to change an optical path difference in a step displacement manner, and changes the distance between the nano probe and the sample to be measured under the optical path difference corresponding to each step position of the moving mirror; a data acquisition module acquires a corresponding near-field interference signal group under each optical path difference; and a data processing module processes the near-field interference signal group to obtain longitudinal resolution infrared spectra of different depth layers. The application can realize the measurement of longitudinal resolution infrared spectra of different depth layers of a sample while maintaining broadband spectrum capability.