311 results about "Nanoprobe" patented technology

The invention discloses an up-conversion nanoprobe for detecting tyrosinase through dual-signal output and application of the up-conversion nanoprobe, and relates to the technical field of nano biological materials, the up-conversion nanoprobe comprises UCNPs, tyramine and iron ions; the UCNPs has a core-shell structure, beta-NaYF4: Yb, Er is taken as a core, and NaYF4 is taken as a shell; according to the up-conversion nanoprobe disclosed by the invention, by integrating the advantages of a fluorescence determination method and a colorimetric method, the dual-signal self-calibration capability is formed; according to the method, the UCL signal excited by near-infrared light is utilized, the detection precision is greatly improved, and an intuitive and convenient method is provided for detection of tyrosinase through colorimetric reaction; and a dual-signal output detection mode effectively relieves signal fluctuation, so that the accuracy and reliability of tyrosinase analysis are ensured.

The invention discloses a reversible background-free hydrogel nanosensor for acetone detection and an intelligent sensing platform, and relates to the technical field of biosensors. The hydrogel nanosensor consists of a hydrogel matrix and an upconversion nanoprobe, and the upconversion nanoprobe comprises upconversion nanoparticles (UCNPs), hydroxylamine sulfate and thymol blue (TB). The invention adopts hydroxylamine sulfate as a specific recognition unit of acetone to construct a reversible luminescent hydrogel probe for acetone detection, and the probe can be reused in multiple detections, thereby reducing the cost of a single detection. In addition, by adopting near-infrared light as an excitation light source, the autofluorescence interference of the hydrogel matrix is ​​effectively avoided, thereby improving the detection accuracy.

The invention discloses a reversible background-free hydrogel nanosensor for acetone detection and an intelligent sensing platform, and relates to the technical field of biosensing, the hydrogel nanosensor is composed of a hydrogel matrix and an up-conversion nanoprobe, the up-conversion nanoprobe comprises up-conversion nanoparticles (UCNPs), hydroxylamine sulfate and thymol blue (TB); according to the present invention, hydroxylamine sulfate is adopted as the specific recognition unit of acetone to construct the reversible luminescent hydrogel probe for acetone detection, and the probe can be repeatedly used in multiple detections so as to reduce the cost of the single detection; in addition, by adopting near-infrared light as an excitation light source, autofluorescence interference of the hydrogel matrix is effectively avoided, and the detection precision is improved.

A method for producing an electrochemiluminescence nanoprobe according to an embodiment includes: a hot exciton nanoparticle synthesis step of polymerizing a hot exciton organic luminescent molecule and a copolymer molecule to synthesize hot exciton nanoparticles; and a hot exciton nanoparticle modification step of modifying the obtained hot exciton nanoparticles with an oligonucleotide chain modified with a quencher molecule to obtain modified hot exciton nanoparticles.

The invention belongs to the field of miRNA detection, and particularly relates to a non-diagnostic-purpose miRNA detection method based on dual cascade amplification of a Pt nanoprobe and a kit. Comprising the following steps: 1) obtaining a Pt NPs (at) Ab nanoprobe; (2) carrying out an ISAR-CRISPR / Cas12a reaction; and (3) dripping on a nucleic acid test strip for capillary action, adding a quenching substrate into the T line for catalysis after the chromatographic reaction is finished, and judging the concentration of miRNA by observing the T line. According to the invention, a Pt nanoprobe is introduced as a fluorescence quenching probe, and after TMB is dropwise added, double-amplification cascade fluorescence quenching is generated. Due to the adoption of a double-amplification cascade fluorescence quenching strategy, the fluorescence quenching efficiency can be greatly improved, so that an ultra-low background fluorescence signal is ensured. The method has a good application prospect, can be used as a general detection method for detecting a specific nucleic acid target, and is widely applied to the fields of nucleic acid detection and the like.

The invention belongs to the technical field of preparation of organic-inorganic hybrid nano materials, and discloses a rare earth composite nano material, a preparation method of the rare earth composite nano material and application of the rare earth composite nano material in preparation of peroxynitrite fluorescent nano probes. The surface of the fluorescent probe is modified with a cyanine dye containing sulfonate for sensitizing rare earth nano-particles, and the rare earth nano-particles are wrapped with an amphiphilic surfactant for surface modification, so that the water-soluble excitation ratio type fluorescent nano-probe is obtained. The fluorescent nanoprobe can be applied to high-resolution imaging of in-vivo blood vessels; meanwhile, the dye in the nanoprobe can generate specific response with OONO <-> ions, the fluorescence emission intensity under the excitation of 808nm laser is reduced, but the fluorescence emission intensity under the excitation of 980nm laser is almost unchanged, and the ratio-type nano fluorescent probe can be constructed by using the two luminous ratio-type changes F1550 and 980Ex / F1550 and 808Ex, and is applied to the detection of OONO <-> ions in basic models such as inflammation and the like.

The invention discloses an acid responsive nanoprobe as well as preparation and anti-tumor application thereof. The nanoprobe has a core-shell structure, takes a nanoparticle self-assembled by a polycation aggregation-induced emission (AIE) photosensitizer and functional nucleic acid as a core, and takes a hyaluronic acid modified metal polyphenol network as a shell. The nanoprobe utilizes the acid responsiveness and broad-spectrum absorption performance of the metal polyphenol network and the diagnosis and treatment advantages of the polycation AIE photosensitizer to realize the acid responsiveness'switch 'controllable diagnosis and treatment of the tumor nanoprobe. In a neutral environment, the fluorescence is weak, the generation of ROS is less, and the background noise signal of the nanoprobe and the toxicity of non-targeted tissues are reduced; in an acid environment, fluorescence is enhanced, the ROS yield is improved, and the treatment efficiency and the curative effect monitoring accuracy are improved. The nano diagnosis and treatment probe is ingenious in design and simple to prepare, and can realize PDT and gene therapy synergistic anti-tumor, in-situ real-time report of early tumor treatment effect and assistance in precise cancer treatment.

The invention provides a label-free magnetic self-assembly nanoprobe, a construction method thereof and application of the label-free magnetic self-assembly nanoprobe in detection of PrPC protein. The label-free magnetic self-assembly nanoprobe disclosed by the invention comprises a magnetic Fe3O4 / Fe2O3 (at) Au heteroplasmon nanorod array and a sulfhydrylation aptamer nucleic acid chain which is combined with the magnetic Fe3O4 / Fe2O3 (at) Au heteroplasmon nanorod array through an Au-S bond; a biosensor prepared from the label-free magnetic self-assembly nanoprobe is good in reproducibility and stability; the RSD value of multiple times of detection (intraday / day) is 1.55%; the storage time of multiple groups of sensors is 0-15 days, and the change of response signal values does not exceed 4%; the biosensor has a relatively low detection limit (1pg / mL), a relatively wide linear range (1 * 10 < pg / mL >-1 * 10 < 5 > pg / mL) and relatively good sensitivity, has very good application in detection of PrPC, and has very good practicability.

The invention discloses a silicon shell protected magnetic quantum dot nanoprobe, a preparation method, a kit and application. The nanoprobe comprises a nanomaterial and a specific antibody, the nanometer material is of a core-shell structure, and the outer surface of a core is sequentially coated with an interlayer and a protective shell layer outwards. The interlayer comprises a multi-layer laminated structure formed by alternately arranging a plurality of layers of positive electricity interlayers and negative electricity interlayers; the positive electric interlayer is polyethyleneimine, the negative electric interlayer is quantum dots, the quantum dots are CdSe / ZnS or carbon quantum dots, amino groups on the surface of the shell layer and a dialkynyl polyethylene ethanol solution are subjected to general coupling of specific antibodies in a click chemistry mode by taking diyne molecules as connecting arms. The magnetic nanoprobe with the core-shell structure not only has strong magnetic responsiveness and a high fluorescence signal, but also can adapt to a complex environment, after the magnetic nanoprobe is integrated with an immunochromatography test strip, to-be-detected targets including pathogenic bacteria and inflammatory markers can be rapidly captured in a whole blood sample at the same time, and the magnetic nanoprobe has huge application potential in early diagnosis of sepsis.

The invention discloses a bimodal polymyxin drug-resistant gene MCR-1 detection platform, relates to the field of food safety and analytical chemistry, and in particular relates to an on-site rapid detection method of the polymyxin drug-resistant gene MCR-1 in a food sample, primer groups aiming at the polymyxin drug-resistant gene MCR-1 are respectively as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5, and a sixth primer with the 5'end labeled by biotin; the CRISPR / dCas9 system comprises dCas9 protein and sgRNA, and the sequence of the sgRNA is marked as SEQ ID NO.7; the sequence of the gold-platinum nanoprobe is marked as SEQ ID NO.8; the LAMP technology is adopted to amplify the polymyxin drug-resistant gene MCR-1 in the sample to be detected, an LAMP product contains multiple sections of reverse circulation repetitive sequences, and each double strand can be combined with more gold-platinum nanoparticles, so that sensitive detection of the food-borne pathogenic bacteria containing the polymyxin drug-resistant gene MCR-1 in the food sample is realized, the sensitivity is relatively high, the detection time is short, the detection time is short, and the cost is low. The operation is simple, the stability is high, and the whole detection process can be completed in a short time.

The invention discloses a colorimetric-fluorescent bimodal nanoprobe for detecting cMyBP-C and a micro-fluidic chip, and belongs to the technical field of biomedical detection and in-vitro diagnosis. The colorimetric-fluorescent bimodal nanoprobe which takes UCNPs as a core and MOF as a shell and is loaded with horse radish peroxidase and a labeled antibody is prepared by providing a layer-by-layer self-assembly technology; the micro-fluidic chip is prepared according to the probe, the micro-fluidic chip can realize cMyBP-C colorimetric and fluorescence signal bimodal detection, and the technical problems of limited sensitivity, susceptibility to background interference, limited linear range, photobleaching phenomenon, signal instability and the like in single signal detection are solved.

The invention belongs to the field of fluorescence detection, and particularly relates to a ratio-type fluorescent nanoprobe for specifically detecting tetracycline antibiotics as well as a preparation method and an application method of the ratio-type fluorescent nanoprobe. The nano-probe is a europium modified zinc gallium germanate nano-material, and is prepared by amino modification, citric acid modification and europium modification of the zinc gallium germanate nano-material. The probe can be specifically combined with tetracycline antibiotics; the fluorescent probe has two different fluorescence emission intensities, so that interference can be effectively eliminated, and the detection accuracy is high. And the synthesis method is simple and low in cost. The ratio type fluorescent nanoprobe provided by the invention also can realize the change of different colors under an ultraviolet lamp, can realize quantitative visual detection of tetracycline by combining the RGB value and the tetracycline concentration change, and can realize rapid on-site detection of tetracycline in a sample without the help of a fluorescent instrument. The tetracycline fluorescent probe is stable in optical performance, not prone to photobleaching, high in tetracycline recognition capacity, wide in response range (0.2-70 [mu] M) and low in detection limit, and has remarkable industrial application potential.

The invention discloses a near-infrared light nano probe as well as a preparation method and application thereof. The preparation method of the near-infrared nano probe comprises the following steps: dissolving polylactic acid-co-acetic acid in an acetonitrile solution, and adding lecithin and 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidyl glycol to obtain a solution A; dissolving indocyanine green in an ethanol solution to obtain a solution B; and mixing the solution A and the solution B, and carrying out ultrasonic treatment to obtain the near-infrared nano probe. The near-infrared light nanoprobe can be used for imaging diagnosis of acute kidney injury, and effectively solves the problems of long diagnosis time, poor stability, high in-vivo clearance rate, low cell absorption rate and the like. Compared with existing ICG nano-particles, the ICG nano-particles are high in biological tissue penetrability, high in resolution ratio, low in phototoxicity and good in fluorescence imaging effect in an acute kidney injury model and have wide application prospects in AKI clinical diagnosis.

The invention relates to the technical field of terahertz biosensing, in particular to a terahertz biosensing detection device and a biological detection method. The system is used for detecting a biological sample and comprises a transmission-type terahertz time-domain spectroscopy system for providing a transmission-type terahertz light path; the metasurface sensor is located on a transmission-type terahertz light path, the metasurface sensor comprises a plurality of first gold films and second gold films of asymmetric structures in a periodic array, and under normal incidence of terahertz waves in the polarization direction, a transmission spectrum harmonic peak is excited to characterize a biological sample; the captured DNA is modified on the surface of the super-surface sensor and is used for capturing a biological sample; the gold nanoprobe is used for being specifically combined with a biological sample after capturing the biological sample so as to enhance the sensing sensitivity to the biological sample. According to the invention, the metasurface sensor and the gold nanoprobe are combined for detection, so that high-sensitivity detection of trace micromolecular biological samples can be realized.

The invention belongs to the technical field of nanoprobes, and particularly relates to a polymyxin B functionalized nanoprobe and a preparation method and application thereof.The nanoprobe comprises magnetic nanoparticles and long-afterglow nanoparticles, the surfaces of the magnetic nanoparticles and the long-afterglow nanoparticles are modified with polymyxin B respectively, and the polymyxin B functionalized nanoprobe is formed by modifying polymyxin B on the surfaces of the magnetic nanoparticles and the long-afterglow nanoparticles. The preparation method of the nanoprobe comprises a method for modifying long afterglow nanoparticles with polymyxin B and a method for modifying magnetic nanoparticles with polymyxin B. According to the nanoprobe disclosed by the invention, the magnetic nanoparticles with separation and enrichment effects and the long afterglow nanoparticles with afterglow luminescence and interference resistance are prepared on the basis of taking antibiotics as recognition molecules; through the enriched and separated magnetic nanoparticles and the long afterglow nanoparticles for providing signals, a new detection means is provided for rapid and quantitative background-free detection of gram-negative bacteria.

To provide an electrochemiluminescent nanoprobe with a wider detection range and high sensitivity.SOLUTION: A method for producing an electrochemiluminescent nanoprobe according to an embodiment includes: a hot exciton nanoparticle synthesis step for polymerizing hot exciton organic luminescent molecules and copolymer molecules to synthesize hot exciton nanoparticles; and a hot exciton nanoparticle modification step for using oligonucleotide strands with quencher molecule modifications to modify the obtained hot exciton nanoparticles, resulting in modified hot exciton nanoparticles.SELECTED DRAWING: Figure 1

The invention provides an activatable head and neck cancer diagnosis and treatment integrated nanoprobe and a preparation method and application thereof.The preparation method includes the steps that according to an original data matrix, an adaptive spectrum correction algorithm is applied to eliminate background noise of an instrument, and when the relative fluctuation of spectral intensity exceeds a preset threshold value by 5%, a sliding window filtering technology is adopted, the window size is 5 nanometers, and the spectrum intensity is obtained; generating a de-noised spectral data set; measuring the pH value of the head and neck cancer tissue microenvironment by using a pH value sensor, recording the emission peak position change of the near-infrared quantum dots when the pH value is less than 6.5 in combination with the de-noising spectral data set, analyzing the frequency band displacement by adopting a Gaussian fitting algorithm, and generating a mapping data set of the pH value and the frequency band displacement; and aiming at the activation spectrum fingerprint data set, applying a dynamic spectrum matching algorithm, comparing with the spectrum response characteristic data set, calculating a cosine similarity coefficient, and when the similarity exceeds 0.85, determining the activation state and the tissue penetration depth of the nanoprobe, and generating a diagnosis and treatment parameter adjustment instruction set.

The invention discloses a method and device for measuring structural parameters of an MEMS cantilever beam, and the method comprises the steps: placing the MEMS cantilever beam on an objective table of a nanoprobe station, and guaranteeing that the thickness direction of the MEMS cantilever beam is perpendicular to a horizontal plane; measuring the length l and the width b of the MEMS cantilever beam by using a microscope; controlling a micro probe of the nano probe station to descend until the micro probe is in contact with the surface of the MEMS cantilever beam; continuously controlling the micro-probe to descend, and recording the displacement X and the loading stress F of the micro-probe at the same time; carrying out linear fitting by utilizing a preset calculation device displacement X and the loading stress F to obtain the deformation rigidity K of the MEMS cantilever beam; and calculating the thickness h of the MEMS cantilever beam based on the deformation stiffness K, the length l, the width b and the Young modulus E. The measuring method is wide in application range and low in testing cost.

The invention discloses a fluorescence-colorimetric bimodal nanoprobe and a biosensor for CAT detection, and relates to the technical field of nano biological materials, the fluorescence-colorimetric bimodal nanoprobe comprises UCNPs, hydrogen peroxide, ferrous ions and neutral red; the preparation method comprises the following steps: firstly, preparing UCNPs through a specific method, and then constructing the fluorescent-colorimetric bimodal nanoprobe by utilizing interaction of hydrogen peroxide, ferrous ions, neutral red and the UCNPs; in a fluorescence mode, the activity of CAT is reflected by measuring the quenching degree of an up-conversion fluorescence signal; in the colorimetric mode, the CAT activity is detected by observing the color fading degree of neutral red; the bimodal detection method not only improves the accuracy and sensitivity of detection, but also realizes visual rapid detection of CAT activity.

The invention discloses a micro-fluidic chip for detecting breast cancer tumor markers and a preparation method and application thereof, and belongs to the technical field of microfluidics, the preparation method of the micro-fluidic chip comprises the following steps: preparing up-conversion nanoparticles with a core-shell structure through a solvothermal method and a homogeneous wrapping method; the preparation method comprises the following steps: carrying out surface ligand-free treatment on an up-conversion nanoparticle with a core-shell structure, and modifying the up-conversion nanoparticle with ssDNA complementary to a breast cancer tumor marker to obtain the UCNPs-ssDNA fluorescent nanoprobe; the preparation method comprises the following steps: spin-coating a ZnO inverse opal photonic crystal with a UCNPs-ssDNA fluorescent nanoprobe to obtain a photonic crystal / UCNP composite structure; and dispensing the MoS2 nanosheet solution on the composite structure of the photonic crystal / UCNP to obtain the micro-fluidic chip. Compared with the traditional detection means, the micro-fluidic chip has the advantages of low cost, real-time visual detection, high detection sensitivity and the like.

The invention discloses a background-free fluorescent-colorimetric bimodal hydrogel for promethazine detection and a portable sensing platform, and relates to the technical field of drug safety and environmental hazard detection, the fluorescent-colorimetric bimodal hydrogel is composed of a hydrogel matrix and a nanoprobe, and the nanoprobe comprises UCNPs and bromate; the nanoprobe containing UCNPs and bromate is embedded into a PAM hydrogel matrix, green up-conversion luminescence of the UCNPs can be quenched by a red product generated by oxidation reaction of PMZ and bromate, and red emission is kept unchanged, so that fluorescence and colorimetric dual-mode detection of PMZ is realized; in addition, the PAM hydrogel matrix provides a liquid detection environment and high loading capacity of the nano probe; the near-infrared light excitation characteristic of the UCNPs eliminates the interference of background fluorescence.

The invention discloses a digital analysis method and system based on nano lens high signal-to-noise ratio holographic imaging, and belongs to the field of biochemical analysis. According to the invention, the microsphere nanoprobe is combined with a high signal-to-noise ratio holographic microscopic imaging technology, the number of microsphere signal probes in a supernatant / immune complex is changed through a series of biochemical reactions, and high signal-to-noise ratio holographic imaging is carried out on the PS microsphere nanoprobe in the supernatant after magnetic separation; in the holographic imaging process, the surface acoustic wave-interdigital transducer is used for driving and forming a high-signal-to-noise-ratio lensless holographic microscope of a nano lens; and obtaining the number of the microsphere signal probes in the holographic image by using a lightweight unsupervised learning target detection algorithm, and finally realizing accurate quantitative detection of the target object to be detected. Experimental results show that the detection sensitivity and efficiency are effectively improved, and meanwhile the detection accuracy is improved.

The invention belongs to the technical field of nano-probes, and particularly relates to a nano-probe and a preparation method and application thereof.The nano-probe comprises a magnetic nano-material and a long afterglow nano-material, the magnetic nano-material comprises magnetic nano-particles modified with concanavalin A and is used for recognizing, capturing and separating bacteria, and the long afterglow nano-material is a long afterglow nano-material. The long-afterglow nano-material comprises green long-afterglow nano-particles of which the surfaces are modified with polymyxin B and red long-afterglow nano-particles of which the surfaces are modified with vancomycin, the green long-afterglow nanoparticles with the surfaces modified with polymyxin B and the red long-afterglow nanoparticles with the surfaces modified with vancomycin are respectively used for identifying anti-interference signal output, the nano-probe is applied to identification of gram-negative and positive bacteria, the anti-interference capability of detection can be improved, the whole process is rapid and simple, and the cost is low. And the cost is low, and the stability is high.

The invention discloses a gold-silver alloy nanoprobe based on double-signal cooperative detection, a preparation method and application thereof, and belongs to the technical field of sensing. Then mixing the gold nanorod, a Raman report molecule p-methylthiobenzonitrile and a chiral stimulating factor L-cysteine, and jointly incubating at room temperature to obtain a mixed solution; sequentially adding silver nitrate, chloroauric acid tetrahydrate and ascorbic acid into the mixed solution, and magnetically stirring; and placing the mixed solution under a constant temperature condition to react for a certain time, so that the gold-silver alloy shell fully grows, and finally obtaining the spiral structure alloy nanoprobe with Raman signals and CD signals. The probe realizes the dual detection functions of Raman signals and circular dichroism signals, and can detect the lactic acid concentration with high sensitivity and high selectivity by combining the specific catalytic action of the lactate oxidase, and meanwhile, the Raman signals and CD signals of the silent zone can further expand the application range of the probe.

The invention discloses a fluorescence-colorimetric bimodal nanoprobe and a biosensor for amikacin sulfate detection, and relates to the technical field of drug safety and environmental hazard detection, the fluorescence-colorimetric bimodal nanoprobe comprises up-conversion nanoparticles and Congo red; the UCNPs is prepared by taking rare earth chloride, oleic acid, 1-octadecene, ammonium fluoride and sodium hydroxide as raw materials through a solvothermal method. According to the invention, a fluorescence method and a colorimetric method are innovatively combined, and the fluorescence-colorimetric bimodal nano probe is designed and is used for real-time analysis and visual detection of AMK; the near-infrared excitation characteristic and the stable chemical property of UCNPs are utilized, background fluorescence interference is reduced, the AMK detection sensitivity, accuracy and anti-interference capacity are remarkably improved, and the method can be applied to AMK detection in actual samples.

The invention discloses a method for detecting silicon dioxide in kaolin based on a nanoprobe. The method comprises the following steps: grinding a kaolin sample, mixing with deionized water, performing ultrasonic dispersion, and centrifuging to obtain a kaolin sample solution; the preparation method comprises the following steps: preparing titanium dioxide nano-particles with amino groups modified on the surfaces by adopting a hydrothermal method, and modifying a silane coupling agent on the surfaces of the nano-particles by adopting a chemical coupling method to form a functionalized nano-probe; adding the functionalized nanoprobe into a kaolin sample solution; the nanoprobe is combined with silicon dioxide in a sample to form a nanoprobe-silicon dioxide compound; collecting a nano probe-silicon dioxide compound; reacting the nanoprobe-silicon dioxide compound with a fluorescent marker, and combining the fluorescent marker with silicon dioxide in the compound; and detecting the fluorescence intensity, and calculating the content of silicon dioxide in the sample according to the standard curve. The method for detecting silicon dioxide in kaolin is high in sensitivity, high in selectivity, simple and convenient to operate and wide in application.

The present invention provides a glutathione-activated co-assembled nanoprobe and a preparation method and application thereof, belonging to the technical fields of chemical synthesis, biological analysis and detection, tumor imaging and treatment. In the present invention, amphiphilic small molecule probes 1-Zn-PPA and 1-NLG are constructed, and the probes 1-Zn-PPA and 1-NLG can be co-assembled in a solution to form co-assembled nanoprobes 1-NPs. The nanoprobes 1-NPs can accumulate at the tumor site under the tumor EPR effect and the targeting action of cRGD on the probe surface, and disassemble under the action of GSH present in the tumor reducing environment to release Gd-containing hydrophilic small molecule 2-Gd, light / sound-sensitive drug Zn-PPA-SH and immune adjuvant drug NLG919. The nanoprobes 1-NPs can realize combined sonodynamic therapy / photodynamic therapy / immunotherapy of living tumors. The nanoprobes 1-NPs have good applications in fluorescence imaging, magnetic resonance imaging, and the preparation of tumor treatment drugs and tumor-killing devices.

The invention discloses a near-infrared two-region dual-channel fluorescent nanoprobe as well as a preparation method and application thereof, and relates to the technical field of biomedical imaging. A nano probe A and a nano probe B included in the near-infrared two-region dual-channel fluorescent nano probe are both of a core-shell structure, two NIR-IIb fluorescent nano probes adaptive to different excitation wavelengths (808 nm and 980 nm) are constructed by precisely regulating and controlling the types and proportions of lanthanide elements, dual-channel imaging is achieved, limitation of a single excitation wavelength is broken through, spectral crosstalk is eliminated, and the near-infrared two-region dual-channel fluorescent nano probe has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method has the characteristics of material uniformity and biological safety. The near-infrared IIb region dual-channel imaging system has the characteristics of high temporal-spatial resolution and signal separation, and has significant application value in the field of living body multi-target synchronous observation. Compared with a traditional two-photon microscope technology, the imaging method has a wider observation view field, a local craniotomy operation does not need to be carried out on an experimental mouse, and physiological trauma is remarkably reduced.

The application belongs to the technical field of luminescent materials, and particularly relates to a nickel ion doped fluoride near-infrared two-region long-wave luminescent nano probe and a preparation method thereof, wherein the nano probe comprises a fluoride nanocrystal matrix and transition metal nickel ions as a luminescent center, or a core-shell structure nano probe formed by taking the above-mentioned substances as a shell layer and being externally coated with an inert shell layer or a homogenous epitaxial layer. The probe realizes wide-range regulation of an emission peak in a 1300nm to 2150nm near-infrared two-region long-wave band; through core-shell structure design and inert shell layer coating, surface defects are effectively passivated, luminescent quantum efficiency is significantly improved, and particle size is precisely controlled. Through nickel ion doping and crystal field regulation strategies, the application develops a new type of non-lanthanide near-infrared two-region long-wave luminescent material; the application has unique dual-wavelength excitation characteristics and wideband emission characteristics, and has wide application prospects in multi-channel biological detection and imaging analysis.

The present invention discloses a large-dynamic scattering terahertz material imaging and composition analysis device and method. The device includes a dual-port frequency-sweep excitation signal generation and intermediate frequency signal detection module, a two-way low-noise amplification and mixing module, a low-noise amplification module, an IQ mixing amplification module, a data acquisition module, and an industrial control computer, all of which are sequentially connected. The dual-port frequency-sweep excitation signal generation and intermediate frequency signal detection module are respectively connected to a terahertz signal frequency-doubling transmission module and a terahertz signal frequency-mixing detection module via a switch matrix. A signal transmission adjustment device, a nanoprobe, a piezoelectric body, a two-port excitation source, and a three-dimensional scanning frame for moving the sample to be tested are provided between the terahertz signal frequency-doubling transmission module and the terahertz signal frequency-mixing detection module. The device and method of the present invention can break through the diffraction limit and achieve nanoscale imaging; possess near-field terahertz scattering spectrum analysis capabilities, realize material composition analysis, and achieve large-dynamic testing with an accuracy exceeding 100dB across the entire frequency band.