125 results about "Nanoprobe" patented technology

The invention provides an activatable head and neck cancer diagnosis and treatment integrated nanoprobe and a preparation method and application thereof.The preparation method includes the steps that according to an original data matrix, an adaptive spectrum correction algorithm is applied to eliminate background noise of an instrument, and when the relative fluctuation of spectral intensity exceeds a preset threshold value by 5%, a sliding window filtering technology is adopted, the window size is 5 nanometers, and the spectrum intensity is obtained; generating a de-noised spectral data set; measuring the pH value of the head and neck cancer tissue microenvironment by using a pH value sensor, recording the emission peak position change of the near-infrared quantum dots when the pH value is less than 6.5 in combination with the de-noising spectral data set, analyzing the frequency band displacement by adopting a Gaussian fitting algorithm, and generating a mapping data set of the pH value and the frequency band displacement; and aiming at the activation spectrum fingerprint data set, applying a dynamic spectrum matching algorithm, comparing with the spectrum response characteristic data set, calculating a cosine similarity coefficient, and when the similarity exceeds 0.85, determining the activation state and the tissue penetration depth of the nanoprobe, and generating a diagnosis and treatment parameter adjustment instruction set.

The invention discloses a near-infrared two-region dual-channel fluorescent nanoprobe as well as a preparation method and application thereof, and relates to the technical field of biomedical imaging. A nano probe A and a nano probe B included in the near-infrared two-region dual-channel fluorescent nano probe are both of a core-shell structure, two NIR-IIb fluorescent nano probes adaptive to different excitation wavelengths (808 nm and 980 nm) are constructed by precisely regulating and controlling the types and proportions of lanthanide elements, dual-channel imaging is achieved, limitation of a single excitation wavelength is broken through, spectral crosstalk is eliminated, and the near-infrared two-region dual-channel fluorescent nano probe has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method has the characteristics of material uniformity and biological safety. The near-infrared IIb region dual-channel imaging system has the characteristics of high temporal-spatial resolution and signal separation, and has significant application value in the field of living body multi-target synchronous observation. Compared with a traditional two-photon microscope technology, the imaging method has a wider observation view field, a local craniotomy operation does not need to be carried out on an experimental mouse, and physiological trauma is remarkably reduced.

The application belongs to the technical field of luminescent materials, and particularly relates to a nickel ion doped fluoride near-infrared two-region long-wave luminescent nano probe and a preparation method thereof, wherein the nano probe comprises a fluoride nanocrystal matrix and transition metal nickel ions as a luminescent center, or a core-shell structure nano probe formed by taking the above-mentioned substances as a shell layer and being externally coated with an inert shell layer or a homogenous epitaxial layer. The probe realizes wide-range regulation of an emission peak in a 1300nm to 2150nm near-infrared two-region long-wave band; through core-shell structure design and inert shell layer coating, surface defects are effectively passivated, luminescent quantum efficiency is significantly improved, and particle size is precisely controlled. Through nickel ion doping and crystal field regulation strategies, the application develops a new type of non-lanthanide near-infrared two-region long-wave luminescent material; the application has unique dual-wavelength excitation characteristics and wideband emission characteristics, and has wide application prospects in multi-channel biological detection and imaging analysis.

The invention relates to an application of a nanosphere fluorescent probe combined CRISPR (clustered regularly interspaced short palindromic repeats) in detection of exosomes, which comprises the following steps: inserting DNA nanospheres as a carrier of fluorescent signal molecule methylene blue into a cholesterol probe on an exosome membrane to start a strand displacement reaction, activating trans-cleavage activity of Cas12a by a reaction product to split the DNA nanospheres wrapped with methylene blue, further, qualitative and quantitative analysis of the exosome is realized through fluorescence signal detection of methylene blue. Compared with a common CRISPR / Cas-based biosensing platform, the nanosphere fluorescent probe and the CRISPR are combined, so that high-sensitivity detection of the exosome can be realized, and the application scene of the nucleic acid nanoprobe and the CRISPR-Cas system in fluorescent biosensing is widened.

The present application relates to the technical field of nanoprobes, and particularly relates to a near-infrared fluorescence / magnetic resonance dual-mode probe and a preparation method and application thereof. The present application adopts a Brij98 amphiphilic molecule to wrap an IR824Et-PhDTA-Gd near-infrared fluorescence / magnetic resonance dual-response molecule to prepare the probe, so that the probe has an absorption wavelength of 650-850 nm, generates a fluorescence signal under excitation of 785 nm laser, and the generated fluorescence wavelength can reach 1000 nm, which is in a near-infrared region (780-1700 nm); the probe has good positive magnetic resonance response characteristics, and a longitudinal relaxation rate (r1) of the probe is 28 mM ‑1 s ‑1 , which is 7 times of that of a clinically used Magnevist; the probe can be applied in the fields of fluorescence and / or magnetic resonance imaging, and has a wide application prospect. The preparation method of the near-infrared fluorescence / magnetic resonance dual-mode probe is simple in operation and low in cost.

The invention relates to the technical field of magnetic particle imaging, in particular to a core-shell nanoprobe and a preparation method and application thereof.The core-shell nanoprobe comprises a magnetic core and a nanoshell which is located outside the magnetic core and has a magnetic shielding response function, and the structure of the nanoshell collapses under the tumor microenvironment condition; the saturation magnetization of the magnetic core is greater than or equal to 50 emu / g, and the coercive force is less than or equal to 5 Oe; the saturation magnetization of the nano shell is less than 50 emu / g, and the coercive force is more than 5 Oe. According to the core-shell nanoprobe, through a unique magnetic core-shell structure design and tumor microenvironment response characteristics, a magnetic shielding layer is formed by using a low-signal nano shell with a response magnetic shielding function, and background signal interference is inhibited. In a tumor microenvironment, the shell structure of the core-shell nanoprobe collapses, the magnetic core is exposed, and a high magnetic signal is released and generated, so that the imaging signal-to-noise ratio is remarkably improved. According to the invention, the bottleneck that the traditional MPI probe is lighted in the whole course can be broken through, the tumor specificity is lighted and positioned, and the problems that the external background of the tumor is high, the contrast ratio is poor, and tiny focuses are difficult to detect are solved.

The invention discloses an MMP-9-targeted MRI (Magnetic Resonance Imaging) nanoprobe and a preparation method and application thereof, the MMP-9-targeted MRI nanoprobe is represented by Gd-ACC-MAP and comprises amorphous calcium carbonate and an aptamer covalently connected to the surface of the amorphous calcium carbonate, the calcium carbonate is doped with gadolinium, and the aptamer has a nucleotide sequence as shown in SEQ ID NO: 1. The MRI nanoprobe can be used for early diagnosis of aneurysm and evaluation of rupture risk of the aneurysm.

The invention relates to the technical field of biology, and discloses a nano composite probe as well as a preparation method and application thereof. The nano composite probe comprises an iron-based metal organic framework, glucose oxidase loaded on the surface of the iron-based metal organic framework, and an anti-cTnI antibody coupled with the glucose oxidase. According to the nano-composite probe, glucose oxidase, an acid-responsive iron-based metal organic framework and a detection antibody are subjected to function integration, so that three-in-one of recognition, driving and feedback is realized; a cardiac troponin I detection method based on a nanoprobe triggered enzyme catalysis-MOF disintegration-Fenton reaction self-amplification cascade system innovatively adopts a strategy of etching a DNA protective layer by hydroxyl radicals to regulate and control gold nanoparticle salt-induced aggregation, and finally reads signals through dynamic light scattering, so that ultrahigh-sensitivity detection is realized, the detection limit is as low as 1.5 * 10 <-13 > g / mL, and the detection time is greatly shortened. The stability and the specificity are excellent.

The application discloses a kind of near-infrared afterglow nano probe and its preparation method and application, wherein the near-infrared long afterglow nano probe is near-infrared long afterglow nano probe, particle size is 100nm, and its composition is: ZnGaSnO4:Cr, Y, wherein, the ratio of Zn, Ga, Sn, Cr, Y is 1.3:1.4:0.3:0.005:0.003, the near-infrared long afterglow nano probe of the application has higher luminous intensity and excellent fluorescent performance, is a new type of luminescent material, can emit light for more than several hours under external environment, has high luminous intensity, long life and good chemical stability and other characteristics, has the advantages of low background, strong fluorescent signal, high signal-to-noise ratio and the like, label is connected with oxidized low density lipoprotein monoclonal antibody by covalent bond, and the labeled product is stable, has the characteristics of high sensitivity, high accuracy, rapid and simple detection and the like, early diagnosis, curative effect monitoring and prognosis evaluation of coronary heart disease and atherosclerosis can quickly help clinical diagnosis.

The application discloses a kind of near-infrared benzothiadiazole dye sensitized rare earth upconversion luminescence nano-probes and preparation method thereof;Probe is composed of near-infrared benzothiadiazole dye and upconversion nanoparticle, benzothiadiazole dye is compounded with ligand-free lanthanide ion doped upconversion nanoparticle, and upconversion luminescence is enhanced.This application preparation method is simple, and it does not involve complex core-shell structure and energy migration process, benzothiadiazole dye is directly compounded with ligand-free rare earth nanoparticle, and upconversion luminescence nano-probe is formed, under the irradiation of 808nm laser, the emission spectrum of benzothiadiazole dye and the absorption spectrum of sensitizer ion in upconversion nanoparticle overlap, which can effectively enhance the upconversion luminescence intensity of nano-probe, and keep good light stability, keep more than 90% of initial luminescence intensity under the irradiation of 120 minutes excitation light.

The application relates to the technical field of long-afterglow materials, and particularly provides a preparation method of a near-infrared long-afterglow luminescent nano probe and application thereof. The preparation method of the near-infrared long-afterglow luminescent nano probe comprises the following steps: dissolving water-soluble zinc salt, water-soluble tin salt, water-soluble gallium salt, water-soluble chromium salt and water-soluble yttrium salt in water to prepare a metal mixed solution with a salt concentration of 0.1-2 mol / L; adding mesoporous silica powder into the metal mixed solution, uniformly dispersing, stirring for 30 min-48 hours, adding an alkaline precipitant drop by drop until the pH is 8-10, collecting the precipitate, drying, high-temperature calcining, and obtaining doped particles; and coating manganese dioxide on the surface of the doped particles, and the near-infrared long-afterglow luminescent nano probe has the characteristics of slow afterglow decay.

The invention discloses a Candida albicans specificity detection kit and application thereof, the kit comprises a nanoprobe, the nanoprobe takes a gold nanostar as a substrate, and the surface of the substrate is modified with Raman probe molecules and a Candida albicans specificity aptamer. The kit has high sensitivity, specificity, reproducibility, uniformity and stability, Raman spectrum can be collected through portable Raman spectrum equipment, the kit can be used for analyzing trace Candida albicans in a sample, and the detection limit is 1.14-1.21 CFU / mL. Compared with the traditional method, the detection method of the kit has the advantages of simple pretreatment process, short detection time, simplicity in operation and the like, obviously reduces the time and economic cost, and is more suitable for on-site rapid detection of candida albicans.

The invention relates to a magnetic quantum dot composite nanoprobe as well as a preparation method and application thereof. The composite nanoprobe comprises a composite nanomaterial and a specific antibody, the composite nano material has a core-shell structure; the core of the core-shell structure is Fe3O4 nano-particles, and the outer surface of the core is externally coated with an interlayer and a shell layer in sequence; the interlayer is of a multi-layer laminated structure formed by positive electricity interlayers and negative electricity interlayers alternately, the first layer in the laminating direction of the laminated structure is the positive electricity interlayer, and the last layer in the laminating direction of the laminated structure is the negative electricity interlayer; the positive electricity interlayer is made of polyethyleneimine; the negative electricity interlayer is quantum dots; the shell layer is SiO2 modified by carboxyl; the carboxyl of the shell layer is coupled with a specific antibody. The magnetic nanoprobe disclosed by the invention has strong magnetic responsiveness, high fluorescence signal and specific bacterium capturing capacity, realizes rapid capturing of to-be-detected substances such as food-borne pathogenic bacteria in a to-be-detected sample, and has huge application potential in the field of rapid detection of food-borne bacteria.

The application discloses a pure titanium nanometer scale oxide layer nanometer probe precise detection method, which comprises the following steps: S1, preparing a titanium sample to be measured, and pretreating the titanium sample to be measured to make the surface of the titanium sample to be measured smooth; S2, selecting multiple test points on the surface of the titanium sample to be measured and marking the test points, using a pressure head of a nano indentation instrument to make an indentation on the marked test points, and obtaining surface indentation data; S3, selecting multiple test points on the cross section of the titanium sample to be measured and marking the test points, using the pressure head of the nano indentation instrument to make an indentation on the marked test points, and obtaining cross section indentation data; S4, summing up the maximum contact depth in the surface indentation data of each test point and taking an average value, and marking the average value as a; summing up the maximum contact depth in the cross section indentation data of each test point and taking an average value, and marking the average value as b; and taking a difference between a and b to obtain an influence value of the oxide layer on the titanium surface. The application can simply and quickly evaluate the influence of the oxide layer on the titanium surface by using the nano indentation method.

The invention belongs to the technical field of fluorescence detection, and particularly relates to a ratio-dependent up-conversion fluorescence nanoprobe for veterinary drug detection and a preparation method and application of the ratio-dependent up-conversion fluorescence nanoprobe. The invention provides a ratio type up-conversion fluorescent nanoprobe for veterinary drug detection. The ratio type up-conversion fluorescent nanoprobe comprises a core nanoparticle NaGdF4: Yb / Tm, an inner layer shell NaYbF4, a middle layer shell NaGdF4: Yb / Er and an outer layer shell NaYF4 from inside to outside, after the fluorescent probe is incubated with furaltadone and malachite green, the fluorescence intensity is quenched, the selectivity is good, and the fluorescent probe can be used for detecting furaltadone and malachite green; furthermore, as a ratio-type up-conversion fluorescent nanoprobe, the fluorescent nanoprobe can be excited by low-energy near-infrared light, does not cause light damage, has higher biological tissue penetration depth, no background fluorescence interference and higher signal-to-noise ratio, and is a fluorescence detection method for veterinary drug residues with excellent reliability; therefore, the technical problem of low reliability of fluorescence detection of veterinary drug residues in the prior art is solved.

The invention discloses a dual-response type near-infrared two-region quantum dot nanoprobe as well as preparation and application thereof, and belongs to the technical field of biomedical nanomaterials and molecular imaging. The quantum dot nanoprobe comprises an inner core and a shell, wherein the shell is coated outside the inner core; the inner core comprises a near-infrared emission quantum dot core, and the surface of an NIR-II quantum dot is connected with a response peptide and a quencher; the shell is prepared from a polylactic acid-glycolic acid copolymer, polylactic acid, polycaprolactone, a polylactide-polyethylene glycol copolymer, a polylactide-polycaprolactone copolymer, a polysulfide block copolymer and the like. According to the NIR-II quantum dot nanoprobe disclosed by the invention, a response peptide chain and a fluorescence quencher are introduced through chemical modification, and a signal off-on function is realized, so that an activated fluorescent probe with high specificity and high contrast is constructed; the nano-composite particle with controllable particle size, adjustable fluorescence and stable structure is formed, has targeting property, responsiveness and biocompatibility, and is suitable for accurate real-time imaging in a complex in-vivo environment.

The application discloses a sample fixing mechanism for nano probe testing, a nano probe testing device and a sample testing method. The sample fixing mechanism comprises a base provided with a first assembly surface; a fixer provided with a second assembly surface matched with the first assembly surface, and further provided with a fixing surface opposite to the second assembly surface, the fixing surface being used for bonding and fixing the sample; and a locking structure provided with a locked state and an unlocked state, the locking structure being capable of relatively fixing the fixer with the base in the locked state, and the fixer being capable of being removed from the base in the unlocked state. The sample is firmly pasted with the fixer after the glue is melted by high-temperature heating, then the second assembly surface of the fixer is matched with the first assembly surface of the base, the fixer is relatively fixed with the base by the locking structure, then the sample is put into a nano probe machine, nano probe testing is carried out, and testing data of the sample is obtained.

The invention discloses a phototherapy sensitization near-infrared two-zone diagnosis and treatment integrated nanoprobe as well as a preparation method and application thereof, and belongs to the technical field of near-infrared two-zone diagnosis and treatment integrated probes. The invention provides a micromolecular fluorescent molecular probe with near-infrared two-region fluorescence emission, which has a structure as shown in the specification, and an amphiphilic NIR-II fluorescent molecule is formed by further modifying a polyethylene glycol chain through a condensation reaction. Aiming at the problem that the single phototherapy effect is limited, phototherapy sensitization is realized through self-assembly entrapment of an IRE1 inhibitor.

A method for preparing bimodal nanocapsules includes: preparing a solution of mesoporous silica and deionized water, adding chloroauric acid solution and stirring, then adding sodium borohydride solution under ice bath stirring conditions to obtain gold nanoparticle-modified mesoporous silica; preparing a solvent of ultrapure water and gold nanoparticle-modified mesoporous silica, then sequentially adding a fluorescence quencher, a fluorescent molecule, and 4-mercaptophenylboronic acid to react separately to obtain bimolecularly modified nanoprobes; and mixing an adenosine-5′-triphosphate nucleic acid aptamer solution with the nanoprobes using ultrapure water as a solvent to obtain nucleic acid aptamer-encapsulated bimodal nanocapsules. This invention provides a simple, mild, environmentally friendly, and low-cost preparation method. The bimodal nanocapsules prepared by this invention can simultaneously monitor the levels of hydrogen peroxide and adenosine-5′-triphosphate (ATP) in cells using fluorescence and surface-enhanced Raman scattering (SERS) techniques, which can be used for early disease monitoring.

The invention relates to the technical field of nano biosensors, and discloses a bimodal nano probe for uric acid detection as well as a preparation method and application of the bimodal nano probe. The bimodal nano-probe is a fluorescent-photo-thermal bimodal nano-probe, is a core-shell structure nano-particle, and sequentially comprises the following components from the inner layer to the outer layer: a) a fluorescent core which is composed of a carbon dot and a silicon dioxide layer coating the carbon dot; b) a manganese dioxide layer coated outside the fluorescent inner core; and c) a molecularly imprinted polymer layer coated outside the manganese dioxide layer. According to the bimodal nanoprobe disclosed by the invention, through ingenious structural design, a fluorescence signal source (carbon dots), a signal modulation and photo-thermal unit and a specific recognition layer (uric acid molecularly imprinted polymer) are integrated, so that bimodal sensing of'specific recognition-> MnO2 etching-> fluorescence recovery / photo-thermal weakening 'on UA is realized; the problems of poor selectivity, complicated operation and high cost of a traditional method are effectively solved.

This application provides a nanoprobe-based electrophysiological signal and biochemical biomarker detection system, comprising: a patch-clamp amplifier; a working electrode electrically connected to a signal acquisition module, the surface of which, except for the tip, is covered by an insulating protective layer, forming a GΩ-level impedance seal after implantation into the cell; a counter electrode connected to a BATH port, with its active end placed in the matrix environment of the target cell, forming a current loop; a reference electrode connected to a REF port, with its active end placed in the matrix environment of the target cell to provide a potential reference point; the patch-clamp amplifier is configured to operate in voltage-clamp mode, with the REF port connected to the reference electrode as a voltage sensing terminal, and the BATH port connected to the counter electrode as a current output terminal, simultaneously acquiring the electrophysiological signal of the target cell and the current signal corresponding to the concentration of the target biochemical biomarker. This application provides a powerful analytical tool for a deeper understanding of the electrochemical signal coupling mechanism of neurons.

The present invention belongs to the technical field of biomedical detection and in-vitro diagnosis. Disclosed are a colorimetric-fluorescent dual-mode nanoprobe and microfluidic chip for detecting cMyBP-C. In the present invention, a colorimetric-fluorescent dual-mode nanoprobe, which comprises UCNPs as a core and MOF as a shell and is loaded with horseradish peroxidase and a labeled antibody, is prepared by means of a "layer-by-layer self-assembly" technique; and then a microfluidic chip is prepared on the basis of the nanoprobe. The microfluidic chip realizes dual-mode detection of colorimetric and fluorescent signals of cMyBP-C, thereby solving technical problems such as limited sensitivity, susceptibility to background interference, limited linear range, photobleaching and signal instability in single-signal detection.

The invention relates to the technical field of biomedical nanomaterials, and particularly discloses a chemiluminescence afterglow nanoprobe based on magnetic regulation and control as well as a preparation method and application of the chemiluminescence afterglow nanoprobe. The nanoprobe takes zinc-doped ferroferric oxide (ZnFe2O4, ZF) magnetic nanoparticles with enzyme-like catalytic activity as an inner core, a chemiluminescent molecule QM-CF with a singlet oxygen response characteristic is wrapped, and the nanoprobe is formed by packaging an amphiphilic material DSPE-PEG-2000. According to the nanoprobe, under the action of an alternating magnetic field, a ZF core can be catalyzed to generate singlet oxygen, so that QM-CF molecules are efficiently activated to generate chemical afterglow luminescence with extremely high intensity and long half-life period; and meanwhile, the ZF kernel endows excellent T2 weighted magnetic resonance imaging capability and magnetocaloric effect. According to the invention, remote and controllable activation and enhancement of chemical afterglow luminescence through an external magnetic field are realized, a multi-mode imaging function integrating magnetic resonance imaging, magnetic afterglow optical imaging and thermal imaging is successfully integrated, and a novel tool is provided for precise diagnosis and imaging guide treatment of solid tumors.

The present application relates to a kind of NIR-IIb window excitation fluorescence imaging capsule endoscope systems, including NIR-IIb window excitation capsule endoscope, nanoprobes and image receiving and processing module;Capsule endoscope is encapsulated integrated with light source and image acquisition module, microcontroller, wireless communication module, power module and motion control module;Light source and image acquisition module include multiple white light LED, NIR-IIb laser diode and miniature camera;After excitation by NIR-IIb light source, the specific green fluorescence of pathological tissue is emitted by the upconversion nanoprobes absorbed;Image receiving and processing module are connected with microcontroller by wireless communication module, for issuing control instruction to microcontroller, receive the image signal collected by miniature camera and handle.Compared with prior art, the present application can realize high penetration depth, high contrast and high signal-to-noise ratio detection of gastrointestinal lesion tissue under the premise of non-invasive.

Disclosed is a wavelength-adjustable stimulated-emission nanoprobe, comprising an Au nanorod, a dye molecule, a mesoporous silica shell layer, and a hyaluronic acid encapsulation layer. The Au nanorod is used as a plasmonic resonant cavity of the stimulated-emission nanoprobe, and the dye molecule is used as a gain medium. By regulating and controlling the energy transfer between the gain medium and the plasmonic resonant cavity, a stimulated-emission nanoprobe having a pump threshold of 0.5 mJ / cm2, a stimulated-emission spectrum linewidth of 3-10 nm, and a wavelength that can be flexibly adjusted is prepared. The probe has the advantages of overcoming the limitation of a relatively wide spectral linewidth of traditional fluorescent probes , and solving the problem that the wavelength of a stimulated-emission nanoprobe is difficult to adjust, thus opening up new avenues for realizing multi-channel imaging in a complex biological environment.

This invention discloses an internal standard-assisted petal-shaped SERS nanoprobe, its preparation method, and its applications. The internal standard-assisted petal-shaped SERS nanoprobe of this invention is a core-molecule-shell structure gold nanoprobe consisting of a gold nanosphere core, an internal standard modifying molecule, a petal-shaped gold nanoshell, and a recognition molecule, 4-nitrobenzenethiophenol. The internal standard modifying molecule is a Raman signal molecule with nitrile, alkyne, or azide functional groups. This invention uses a Raman signal molecule as an internal standard modifying molecule to modify the gold nanosphere core. The corrected working curve exhibits good reproducibility and small error, improving the reliability of the detection results. Growing a petal-shaped gold nanoshell on the molecular layer of the internal standard modifying molecule enhances the SERS activity both inside and outside the SERS substrate, and the petal-shaped shell increases the surface area of ​​the SERS substrate. Furthermore, the recognition molecule of this invention enables ratiometric Raman detection of hydrogen sulfide, effectively reducing background signal interference and improving detection sensitivity.

The invention relates to the technical field of chip processing, in particular to a wafer new process SPICE determination method, which comprises the following steps of: S110, determining items and characteristic parameters which need to be tested by the wafer new process SPICE; s120, acquiring a target chip with the same manufacturing process as the new wafer process, and acquiring a hierarchical structure of the target chip; s130, removing the target chip layer by layer from one side, sequentially obtaining each target layer of the target chip, and positioning a target structure of a to-be-tested item of the target layer and a target position of the target structure on the target layer; s140, according to the determined target position, testing by using a nanoprobe imaging analysis system, and collecting characteristic parameters of the target structure; and S150, after the target structures of all the target layers are tested, obtaining the SPICE of the target chip wafer process. According to the method provided by the invention, the SIPCE of a new wafer process can be quickly established, a guidance direction is provided for developing a new wafer manufacturing process, and meanwhile, a method for quickly obtaining a certain SPICE test result at low cost is provided.

This invention discloses a bimetallic SERS nanoprobe based on a "bioquiescent" region, its preparation method, and its applications. The sensor uses AuNS@4-MBN@Ag-aptamer as the nanoprobe and Fe3O4@AuNPs-cDNA (FA-cDNA) as the magnetic substrate. In this sensor, FA-cDNA and AuNS@4-MBN@Ag-aptamer form a magnetic complex, FA-cDNA-aptamer-AuNS@4-MBN@Ag, through base complementarity pairing. The SERS sensor established using this bimetallic nanoprobe in a "bioquiescent" region has significant practical application value in the detection of trace CPF in complex matrix environments.

The invention relates to a fluorescence imaging capsule endoscope system excited by an NIR-IIb window. The fluorescence imaging capsule endoscope system excited by the NIR-IIb window comprises a capsule endoscope excited by the NIR-IIb window, a nanoprobe and an image receiving and processing module, a light source and image acquisition module, a microcontroller, a wireless communication module, a power supply module and a motion control module are packaged and integrated in the capsule endoscope; the light source and image acquisition module comprises a plurality of white light LEDs, an NIR-IIb laser diode and a micro camera; after being excited by an NIR-IIb light source, the lesion tissue absorbing the up-conversion nanoprobe specifically emits green fluorescence; and the image receiving and processing module is connected with the microcontroller through the wireless communication module, and is used for issuing a control instruction to the microcontroller, receiving an image signal acquired by the micro camera and processing the image signal. Compared with the prior art, the invention can realize strong penetration depth, high contrast and high signal-to-noise ratio detection of gastrointestinal tract lesion tissues on the premise of no damage.

The invention relates to the technical field of medical detection, in particular to a nanoprobe for simultaneously detecting and inhibiting VCAM-1mRNA expression as well as a preparation method and application of the nanoprobe. The nano particle takes a nano Au particle as a core, and p-mercaptocyanophenyl (4-MBN) serving as a Raman signal internal standard molecule is modified on the surface of the Au core; the nano particle takes a gold layer as a shell, and a VCAM-1mRNA targeting recognition sequence segment is fixed on the Au shell; and the tail end of the VCAM-1 mRNA targeted recognition sequence segment is modified with a Raman reporter molecule Cy5. The recognition sequence segment on the nanoprobe can be specifically combined with VCAM-1mRNA to form a rigid double strand, so that Cy5 is far away from the surface of the gold core-shell nanoparticle, the SERS signal strength of Cy5 is weakened, the position of 4-MBN located on a spacing layer is unchanged, and the SERS signal of the 4-MBN is also unchanged. The expression of the VCAM-1 protein can be inhibited by the accompanying mRNA consumption in the detection process, so that the synchronous monitoring and treatment intervention of the atherosclerosis disease closely associated with the expression of the VCAM-1 are realized.