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52 results about "Cellular localization" patented technology
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Localization, in cellular communication, is the technique of pinpointing the exact region or geographic position of a user. Localization is done by cellular servers by collecting the unit (or cellular) data of a SIM through signal towers and then correcting the precise location by various algorithms whereby error is removed by probability.
ERK ligands and polynucleotides encoding ERK ligands
ActiveUS20090186379A1Prevents ERK substrate phosphorylationPeptide/protein ingredientsAntibody mimetics/scaffoldsNucleotideKinase
The invention relates to kinase inhibitor ligands and polyligands. In particular, the invention relates to ligands and polyligands that modulate ERK activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands and polyligands to a cellular localization signal, epitope tag and / or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.
Owner:PRECIGEN INC
Endoplasmic reticulum localization signals
The invention relates to cellular localization signals. In particular, the invention relates to endoplasmic reticulum localization signals in monomeric or multimeric form. The localization signals are utilized as research tools or are linked to therapeutics. Disclosed are methods of making and using polypeptides and modified polypeptides as signals to localize therapeutics, experimental compounds, peptides, proteins and / or other macromolecules to the endoplasmic reticulum of eukaryotic cells. The polypeptides of the invention optionally include linkage to reporters, epitopes and / or other experimental or therapeutic molecules. The invention also encompasses polynucleotides encoding the localization signals and vectors comprising these polynucleotides.
Owner:PRECIGEN INC
Expression cloning processes for the discovery, characterization and isolation of genes encoding polypeptides with a predetermined property
The present invention is directed to a method for detection, characterization and isolation of nucleic acids encoding proteins of a desired property, such as a particular cellular localization. The invention further provides for rapid expression of such proteins or glycoproteins in mammalian cells and for facilitated purification of the novel secreted proteins or glycoproteins. Further, the invention provides for radioactive labelling of the novel proteins or glycoproteins, for rapid identification of sites of binding including animals and for rapid production of infective viral vectors for use in gene transfer.
Owner:CYTOS BIOTECHNOLOGY AG
Rhodamine fluorescent probe sensitive to pH change, synthetic method and application
InactiveCN102659744AEasy to synthesizeHigh sensitivityOrganic chemistryMaterial analysis by observing effect on chemical indicatorCellular MicroenvironmentSolubility
The invention relates to a class of rhodamine fluorescent probe sensitive to pH change. The fluorescent probe has the following structural general formula, the molecules of the fluorescent probe have a simple synthetic process and better chemical and light stability, fluorescence is sensitive to pH change, and the fluorescent probe has different protein kinase A (pKa) values by introducing different amine groups. Meanwhile, the rhodamine fluorescent probe sensitive to the pH change has very good water solubility and biological compatibility and higher fluorescent quantum yield and has the function of cellular localization. Meanwhile, the pH value of a cellular microenvironment can be detected.
Owner:DALIAN UNIV OF TECH
Compositions and methods for efficient targeting of transgenes
ActiveUS20170175131A1Effective controlNot to damageOther foreign material introduction processesOxidoreductasesTransgeneRecombinant DNA
The invention provides recombinant DNA molecules and constructs useful for providing efficient transgene sub-cellular localization of proteins in transgenic plants. Recombinant DNA molecules and constructs for conferring herbicide tolerance or resistance to plants are further provided, as well as plants exhibiting herbicide tolerance and methods for producing or utilizing such plants.
Owner:MONSANTO TECH LLC
Endoplasmic reticulum localization signals
Owner:PRECIGEN INC
Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide
Owner:PRECIGEN INC
Inflammasome activity reporting system for sub-cellular localization and application thereof
InactiveCN104017818AImprove activation efficiencyImprove responseMicrobiological testing/measurementOxidoreductasesInterleukin-1betaCloning Site
The invention belongs to the field of biomedicine, and relates to a fusion protein for sub-cellular localization and an application thereof. A system can be used for reporting the activity of inflammasomes on the organelle level mainly based on secretion type luciferase. 7 kinds of plasmids included in the system are mainly obtained by sequentially cloning an organelle localization gene, an interleukin 1beta precursor coding gene (pro-IL-1beta) and secretion type luciferase (DN-Gluc) to a multiple cloning site zone of an expression vector pcDNA3.1. The plasmids included in the system are transfected to target cells respectively, and the activity of the inflammasomes can be quantitatively analyzed by detecting the activity of luciferase in extracellular supernate, so that the system has the advantages of high efficiency, fastness, simplicity, convenience and the like and can be applied to medicament screening, molecular mechanisms, live animal research and development of related products related to the inflammasomes.
Owner:SUN YAT SEN UNIV
Cellular localization unit, array, and device and formation method of cellular localization unit, array, and device
InactiveCN104789443AHigh precisionHigh sensitivityBioreactor/fermenter combinationsDielectrophoresisDielectrophoretic forceMicroelectrode
The invention provides a cellular localization unit, array and device and a formation method of the cellular localization unit, array and device. The cellular localization unit comprises a substrate and a cellular localization hole. At least one pair of microelectrodes is arranged on the substrate; the microelectrodes are separated and each pair of the electrodes is symmetrically dispersed relatively to the same point to generate a normal dielectrophoretic force area at an area where the ends of the microelectrodes gather; and voltage signals connected to adjacent microelectrodes have opposite phases. The cellular localization hole is located above the microelectrodes; the accommodation room of the cellular localization hole exposes the normal dielectrophoretic force area; and the accommodation room is for a single cell. AC signals with certain amplitudes, frequencies and phases are applied to the microelectrodes, so that cells move towards the surfaces of the microelectrodes, and the cellular localization hole matches the microelectrodes to allow a single cell to be accurately localized at a certain position.
Owner:苏州龙达生物科技有限公司
Recombinant live attenuated foot-and-mouth disease (FMD) vaccine containing mutations in the L protein coding region
ActiveUS8846057B2Reduce severityReduce probabilitySsRNA viruses positive-senseViral antigen ingredientsNeutralizing antibodyAttenuated Live Vaccine
Previously we have identified a conserved domain (SAP, for SAF-A / B, Acinus, and PIAS) in the foot-and-mouth disease virus (FMDV) leader (L) protein coding region that is required for proper sub-cellular localization and function. Mutation of isoleucine 55 and leucine 58 to alanine (I55A, L58A) within the SAP domain resulted in a viable virus that displayed a mild attenuated phenotype in cell culture, along with altered sub-cellular distribution of L and failure to induce degradation of the transcription factor nuclear factor kappa-B. Here we report that inoculation of swine and cattle with this mutant virus results in the absence of clinical disease, the induction of a significant FMDV-specific neutralizing antibody response, and protection against subsequent homologous virus challenge. Remarkably, swine vaccinated with SAP mutant virus are protected against wild type virus challenge as early as two days post-vaccination suggesting that a strong innate as well as adaptive immunity is elicited. This variant could serve as the basis for construction of a live-attenuated FMD vaccine candidate.
Owner:UNITED STATES OF AMERICA
Reagents, methods, and kits for the classification of cancer
Reagents, methods, and kits for the classification of cancer that comprise or employ antibodies that bind specific regions of CD43. One method includes contacting tissue with an antibody capable of specifically binding the cytoplasmic tail of CD43, contacting the tissue with an antibody capable of specifically binding the extracellular domain of CD43, and resolving cellular localization of any binding to the tissue with the antibody capable of specifically binding the cytoplasmic tail of CD43 and the antibody capable of specifically binding the extracellular domain of CD43. The binding patterns of the antibodies can be used to characterize cancer as more aggressive or less aggressive and can distinguish small cell lung cancer from non-small cell lung cancer. The cancer may therefore be treated in accordance of the characterization.
Owner:GUNDERSEN LUTHERAN HEALTH SYST
Models of prion disease
The present invention provides a novel PrP protein, and nucleic acids encoding this protein, where the PrP protein is characterized in vivo by 1) incomplete glycosylation relative to glycosylation of wild-type PrP<C >and 2) proper cellular localization, i.e. an ability to be transported to the cell surface. This novel, under-glycosylated PrP, unlike its normal cellular counterpart, can easily be converted into a protease-resistant isoform by incubation with infectious prions. The invention further provides systems for the study of prion disorders and methods of using these systems, e.g. the study of the mechanical processes in progression of prion-mediated disease or the identification of new therapeutic agents for treatment of prion-mediated disorders. In such systems, protease-resistant under-glycosylated PrP is generated de novo and can be detected by standard immunoblot techniques.
Owner:RGT UNIV OF CALIFORNIA
Long-chain non-coding RNA subcellular localization method based on multi-feature information fusion
PendingCN110046714AEfficient use ofKernel methodsCharacter and pattern recognitionRNA SequenceNon-coding RNA
The invention discloses a long-chain non-coding RNA subcellular localization method based on multi-feature information fusion, and introduces a novel method to predict the position of a long-chain non-coding RNA subcellular. The method is characterized in that k-Mer component and triple structure-sequences are fused to express the long-chain non-coding RNA sequence as a vector, so that the sequence and the structure information of the long-chain non-coding RNA are utilized more comprehensively. In order to obtain an optimal feature subset, feature selection is carried out based on variance analysis. In a cross validation experiment of a leave-one method, the accuracy rate of the method reaches 92.38% and is superior to that of a same-class algorithm.
Owner:TIANJIN UNIV
Flow cytometry electrofusion apparatus
InactiveCN102206580AImprove match rateEasy to collectHybrid cell preparationStress based microorganism growth stimulationElectricityPower flow
The invention discloses a flow cytometry electrofusion apparatus. The flow cytometry electrofusion apparatus comprises: a one-to-one cell fusion portion, a fusion cell detecting and sorting portion and a single cell collection portion. The one-to-one cell fusion portion is provided with a plurality of parallel cellular localization channels. Different cells can be adsorbed to ends of the channels, and two different cells can close to each other and be fused. The fused cells are placed until membranes of the different cells are completely fused, and then are put into the fusion cell detecting and sorting portion. The fusion cell detecting and sorting portion comprises a laser light source, a fluorescence detector and a deflector. The fused cells, i.e., red and green double fluorescent stained cells, are collected in a collecting tube. When collecting a fused cell, flow of droplet in the fluorescence detector is stopped. 200 [mu]l of cell culture medium is added to the collecting tube through a cell culture medium accumulator installed above the collecting tube to open the end of the collecting tube. The 200 [mu]l of the cell culture medium containing the fused cell flows into a well of a 96-well plate arranged blow the collecting tube. At this time, the flow of the liquid in a flow chamber is started, and the next fused cell is put into the next well of the 96-well plate. According to the present invention, one-time continuous accomplishment of one-to-one cell fusion can be realized, fusants can be detected correctly after fusing and each fusant can be cultured respectively.
Owner:BEIHANG UNIV +1
Construction of live attenuated Shigella vaccine strains that express CFA/I antigens (CfaB and CfaE) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic E. coli
ActiveUS7759106B2Reduce intrusionBacterial antigen ingredientsBacteriaHeterologousMucosal Immune Responses
Owner:UNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY
Preparing method of carnation leaf protoplast
ActiveCN109628376AIncrease productionImprove completenessCell culture mediaPlant cellsPlant tissueCell integrity
The invention belongs to the technical field of plant tissue and cell culture, and particularly relates to a preparing method of carnation leaf protoplast. With a carnation tissue culture seedling asthe test material, an efficient protoplast preparing method for purification through a low-speed oscillation enzymolysis-sedimentation method is established. The method is convenient to operate and can be implemented all year round, the yield of the protoplast subjected to low-speed oscillation enzymolysis separation and density gradient purification is high and can reach 183*105 p / gFW, the cell integrity is high, and it is ensured that the activity is 75% or above. By means of the method, a foundation is laid for the research on the aspects of gene function authentication, sub-cellular localization, protein interaction and the like of a carnation leaf instantaneous converting system.
Owner:HUAZHONG AGRI UNIV
Transient expression vector carrying NtRBSC1 gene
InactiveCN107058373APrecise positioningEasy for in-depth developmentVector-based foreign material introductionBiotechnologyNicotiana tabacum
The invention belongs to the technical field of biology and in particular relates to a recombinant transient expression vector carrying a small subunit gene of tobacco ribulose-1,5-bisphosphate carboxylase. One transient expression vector NtRBSC1-pFF19-GFP (Green Fluorescent Protein) is constructed by utilizing a tobacco NtRBSC1 gene; after tobacco cells are transformed through the vector, fused protein of fluorescence labeled gene GFP and NtRBSC1 can be transformed into tobacco protoplasts, so that NtRBSC1 protein is subjected to sub-cellular localization by detecting the position of the fused protein through a laser confocal microscope; the position of chloroplast is displayed. Overall, the manner is a simple and accurate method for localizing in living cells and accurate reference evidence can be provided for research on the chloroplast of the tobacco cells and related genes; research on the tobacco NtRBSC1 gene and deep development of functions of the tobacco NtRBSC1 gene are convenient to realize.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC
Isolated culture method for tea leaf suspension single cells
ActiveCN107022519AEasy to separateSuitable growth vigorCell dissociation methodsCulture processSecondary metaboliteCell metabolite
The invention discloses an isolated culture method for tea leaf suspension single cells. The method comprises the following steps: selecting tea leaves; disinfecting aseptic leaves; inducing healing tissues; culturing liquid suspension cells; performing enlarged culture on the liquid suspension cells; separating the single cells and the like. The tea leaf suspension cells prepared by the method have high growing speed and higher metabolism activity. A rapid-growing suspension cell culture system and a single cell separating method are provided, so that a basis is laid for tea leaf cytological studies and researches of secondary metabolites. A good technical platform is provided for subsequent production of tea tree secondary metabolites, metabolite cellular localization, metabolic mechanism study as well as tea tree cell metabolite change and mechanism study under the stress condition of exogenous compounds.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Endoplasmic Reticulum Localization Signals
The invention relates to cellular localization signals. In particular, the invention relates to endoplasmic reticulum localization signals in monomeric or multimeric form. The localization signals are utilized as research tools or are linked to therapeutics. Disclosed are methods of making and using polypeptides and modified polypeptides as signals to localize therapeutics, experimental compounds, peptides, proteins and / or other macromolecules to the endoplasmic reticulum of eukaryotic cells. The polypeptides of the invention optionally include linkage to reporters, epitopes and / or other experimental or therapeutic molecules. The invention also encompasses polynucleotides encoding the localization signals and vectors comprising these polynucleotides.
Owner:PRECIGEN INC
Increasing lifespan by modulating crtc expression or localization, and methods of screening for modulators of same
The invention relates to the field of longevity enhancement. More particularly, the invention provides compositions and methods relating to CRTC modulation. In certain embodiments, the invention provides compositions and methods for enhancing longevity in an organism by inhibiting CRTC activity, such as, for example, inhibiting CRTC expression or cellular localization in the organism.
Owner:SALK INST FOR BIOLOGICAL STUDIES
Preparation method and application of liposomes of shikonin and derivatives or modifiers thereof
InactiveCN102091037AImprove solubilityLow cytotoxicityOrganic active ingredientsBiological testingFluorescenceCyst
The invention belongs to the fields of pharmaceutical engineering and biotechnology, particularly relates to a prescription process for preparing a shikonin liposome and an effect of the shikonin liposome on resisting generation of new blood vessels. A human umbilical vein endothelial cell (HUVEC) strain is adopted in an anti-angiogenic test, and inhibition (MTT) on a proliferation effect thereof and inhibition (Transwell) on a migration effect thereof are measured. In addition, the invention also relates to shikonin cysts in liposomes, bright red fluorescent light can be observed under the excitation of green fluorescent light, and the shikonin cysts have important application values in the aspects of encapsulation rate measurement, in-vitro cellular localization, endocytosis mechanisms, in-vivo drug tracking and the like. After the shikonin liposomes act on the HUVEC or other tumor cells for a period of time, a confocal microscopy can be used for carrying out cellular fluorescent localization.
Owner:NANJING UNIV
Cytosolically-active peroxidases as reporters for microscopy
ActiveUS20150037829A1Improve enzymatic activityUseful imageSugar derivativesBacteriaElevated enzymesWild type
An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart.
Owner:MASSACHUSETTS INST OF TECH
Methods for the diagnosis or prognosis of breast cancer
InactiveUS20150094224A1Microbiological testing/measurementLibrary screeningSubcellular localizationOncology
Methods for detecting, diagnosing and monitoring an epithelial cancer in a patient are described comprising measuring in a sample from the patient Ep-ICD polypeptides and Ep-ICD polynucleotides. Methods for prognosis of breast cancer comprising measurement of nuclear Ep-ICD polypeptides and optionally EpEx polypeptides are provided. The invention also provides kits and compositions for carrying out the methods of the invention. The invention also provides a unique scoring system using immunohistochemical analysis to arrive at an Ep-ICD Subcellular Localization Index (ESLI) score, which is used to arrive at a diagnosis of cancer in a patient, or, more particularly, to identify patients that are in need of aggressive clinical treatment.
Owner:WALFISH PAUL +1
Helical polycarbodiimide polymers and associated imaging, diagnostic, and therapeutic methods
InactiveUS20160067362A1Easy to controlEasy to modifyPowder deliveryCarbon compoundsRadioactive drugMedicine
Described herein are suspensions of helical polycarbodiimide polymers that ‘cloak’ nanotubes, thereby effecting control over nanotube emission, providing a new mechanism of environmental responsivity, and enabling precise control over sub-cellular localization. The helical polycarbodiimide polymers described herein are water soluble, easily modifiable, and have unique architectures that facilitate their application in radiopharmaceutical delivery and imaging methods, in therapeutics and therapeutic delivery methods, and their use as sensors—both in conjunction with carbon nanotubes, and without nanotubes.
Owner:MEMORIAL SLOAN KETTERING CANCER CENT
Methods of modulating localization and physiological function of IP3 receptors
The present invention relates, generally, to inositol 1,4,5-triphosphate receptors (IP3R), and, in particular, to methods of modulating the activity of 220 kDa ankyrin-B in cellular localization and physiological function of IP3R. The invention further relates to methods of identifying compounds suitable for use in such methods and to compounds so identified.
Owner:DUKE UNIV
Flow cytometry electrofusion apparatus
InactiveCN102206580BImprove match rateEasy to collectHybrid cell preparationStress based microorganism growth stimulationFluorescenceCell culture media
The invention discloses a flow cytometry electrofusion apparatus. The flow cytometry electrofusion apparatus comprises: a one-to-one cell fusion portion, a fusion cell detecting and sorting portion and a single cell collection portion. The one-to-one cell fusion portion is provided with a plurality of parallel cellular localization channels. Different cells can be adsorbed to ends of the channels, and two different cells can close to each other and be fused. The fused cells are placed until membranes of the different cells are completely fused, and then are put into the fusion cell detecting and sorting portion. The fusion cell detecting and sorting portion comprises a laser light source, a fluorescence detector and a deflector. The fused cells, i.e., red and green double fluorescent stained cells, are collected in a collecting tube. When collecting a fused cell, flow of droplet in the fluorescence detector is stopped. 200 [mu]l of cell culture medium is added to the collecting tube through a cell culture medium accumulator installed above the collecting tube to open the end of the collecting tube. The 200 [mu]l of the cell culture medium containing the fused cell flows into a well of a 96-well plate arranged blow the collecting tube. At this time, the flow of the liquid in a flow chamber is started, and the next fused cell is put into the next well of the 96-well plate. According to the present invention, one-time continuous accomplishment of one-to-one cell fusion can be realized, fusants can be detected correctly after fusing and each fusant can be cultured respectively.
Owner:BEIHANG UNIV +1
Cyclin Y and use thereof
InactiveCN101920006APeptide/protein ingredientsMicrobiological testing/measurementKinase activityGenetics
The invention relates to a cyclin Y and use thereof. In the invention, a protein which is the cyclin Y (CCNY) correlative with the adjustment of a cell cycle is determined for the first time. The CCNY can interact with a protein kinase family member PFTAIRE protein kinase 1 (PFTK1) so as to function in control over the kinase activity of the PFTK1 or cellular localization.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Methods and kits for diagnosing and/or prognosing osteoarthritis
InactiveUS20160223570A1High detection sensitivityHigh sensitivityDisease diagnosisBiological testingPediatricsUbiquitins
A method of determining whether a subject is at risk of developing osteoarthritis (OA), said method comprising: determining the cellular localization of a Prohibitin-1 (PHB1) polypeptide and / or Small Ubiquitin-like Modifier (SUMO) polypeptide and / or UBC9, in a cell sample from said subject; and determining whether said subject is at risk of developing OA based on the cellular localization of a PHB1 polypeptide and / or SUMO and / or UBC9 polypeptide, is described.
Owner:VALORISATION HSJ LLP
Methods for the Prognosis of Breast Cancer
InactiveUS20160313335A1Microbiological testing/measurementDisease diagnosisOncologySubcellular localization
Owner:WALFISH PAUL +1
Models of prion disease
The present invention provides a novel PrP protein, and nucleic acids encoding this protein, where the PrP protein is characterized in vivo by 1) incomplete glycosylation relative to glycosylation of wild-type PrPC and 2) proper cellular localization, i.e. an ability to be transported to the cell surface. This novel, under-glycosylated PrP, unlike its normal cellular counterpart, can easily be converted into a protease-resistant isoform by incubation with infectious prions. The invention further provides systems for the study of prion disorders and methods of using these systems, e.g. the study of the mechanical processes in progression of prion-mediated disease or the identification of new therapeutic agents for treatment of prion-mediated disorders. In such systems, protease-resistant under-glycosqvated PrP is generated de novo and can be detected by standard immunoblot techniques.
Owner:RGT UNIV OF CALIFORNIA
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