37 results about "Cellular Microenvironment" patented technology

The invention relates to the field of biological imaging, and particularly discloses an in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging, which comprises the following steps: S1, by using a two-photon frequency domain fluorescence lifetime imaging TP-FD-FLIM technology, losslessly representing microenvironment change through fluorescence lifetime parameters, and monitoring oxidative stress reaction and neutrophil migration behavior in real time, analyzing the effect of the associated compound in combination with an in-vitro cell model; and S2, based on the physical basis of FD-FLIM, establishing a frequency domain fluorescence lifetime signal processing and analysis system under periodic excitation, analyzing the dynamic correlation characteristics of the phase delay phi and the modulation depth M of a fluorescence signal relative to exciting light, and carrying out quantitative backstepping on molecular lifetime parameters. According to the in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging provided by the invention, neuroinflammatory response can be observed in a lossless and real-time manner on the subcellular scale.

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The invention provides a cell classification system and application based on a cell microenvironment map attention network, and the method comprises the following steps: obtaining a mask of each cell nucleus in a TCT image and a centroid coordinate of each cell nucleus, and obtaining a morphological feature vector of each cell nucleus based on each cell nucleus mask, obtaining a depth visual feature vector of each single cell image based on the centroid coordinate of each cell nucleus; constructing an initial cell microenvironment structure chart and carrying out chart attention calculation on the initial cell microenvironment structure chart; and using a classifier to classify the image attention calculation result to obtain the regional cell category of the TCT image. According to the scheme, the cell microenvironment map is constructed based on each cell nucleus in the TCT image, and the node features in the cell microenvironment map are updated by using the map attention mechanism, so that the classification result of the cell nucleus is more accurate, and a key basis is provided for clinical diagnosis and treatment.

The invention relates to the technical field of cosmetics, in particular to a sensitive skin care composition for soothing nerves and a preparation method thereof. According to the sensitive skin care composition capable of soothing the nerves, provided by the invention, the acetyl zingerone clathrate compound, the strawberry fruit extract and the 4-tert-butyl cyclohexanol are compounded, and through the dual-channel synergistic effect of transient inhibition of neural signals and long-acting repair of a cell microenvironment, the synergy of immediate soothing and long-term barrier repair is realized; the vicious circle of sensitive skin is effectively blocked.

The present invention relates to the field of biological autophagy detection technology. The present invention discloses a method and system for detecting mitochondrial autophagy in cells. The method simulates the cell microenvironment by constructing an experimental platform, combines fluorescent probe labeling, dynamic monitoring and gene regulation, and realizes real-time monitoring and quantitative analysis of the dynamic changes of mitochondrial autophagy. Compared with the existing technology, the present invention shows its unique advantages in many aspects: first, through microfluidic technology, the intracellular drug and gene status can be independently regulated to achieve parallel processing of samples and significantly improve detection efficiency; second, the new strategy of quantitative fluorescence imaging and data processing is adopted, which not only improves the reliability of the signal, but also clarifies the selection mechanism of biomarkers, thereby enhancing the accuracy of identifying the autophagic state; the present invention provides a more systematic experimental basis for studying the molecular mechanism of mitochondrial autophagy by comprehensively analyzing the relationship between fluorescence signals and gene regulation.

The invention provides responsive release polypeptide hydrogel. The hydrogel comprises a gel product of a raw material composition containing the following raw material components: (A) a biocompatible polymer containing at least two sulfydryl groups; (B) a matrix metalloproteinase substrate polypeptide; (C) a compound containing a phenolic hydroxyl group structure; (D) a cross-linking agent; and (E) water; the sequence of the matrix metalloproteinase substrate polypeptide is at least one of the following sequences (b1) to (b2): (b1) Cys-Pro-Leu-Gly-Val-Arg-Gly-Arg-Gly-Asp-Ser, (b2) Cys-Pro-Leu-Gly-Val-Arg-Gly-Asp-Ser, (b3) Cys-Pro-Leu-Gly-Val-Arg-Gly (b2), Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-C-ys, and (b3), Dnp-Pro-Leu-Gly- The responsive release polypeptide hydrogel can release polypeptide in a cell microenvironment to promote tissue regeneration and repair, and can be used for preparing muscle-bone healing promoting materials and treating ligament injury diseases such as rotator cuff injury.

The invention belongs to the technical field of organoids, and particularly relates to a method for evaluating the influence of intervention measures such as drugs on the proliferation capacity of intestinal stem cells through proliferation indexes of the organoids and application of the method. The invention discloses a kit for efficiently, simply, conveniently and effectively culturing and constructing intestinal organs and evaluating the multiplication capacity of intestinal stem cells, which can better fit in-vivo tissues and cell microenvironments, as well as a method and application of the kit. The method can be used for detecting the influence of different intervention measures on the proliferation activity of the small intestine stem cells more accurately and more stably, and is beneficial to deeply researching and developing new drugs and researching the drug sensitivity and the drug action mechanism.

The invention discloses a method for low-density amplification of T cells and an obtained product, and belongs to the technical field of cell culture. The method comprises the following steps: activating a cell population containing T cells, and co-culturing with a lentiviral vector carrying a target gene for transduction; the method comprises the following steps: transduction is carried out on cells, then the transduction cells are inoculated into a shake flask at an inoculation density of not higher than 2 * 10 < 5 > cells / mL, shake culture is carried out to realize amplification, and amplification of 30-120 times can be realized. According to the method, a low-density cell microenvironment is optimized through dynamic oscillation, the problems of signal insufficiency, factor dilution and metabolism imbalance are solved, the efficiency bottleneck of traditional low-density amplification is broken through, and the method is suitable for large-scale production. The method has the beneficial effects of simplicity in operation, high cell product viability, high purity, low cost, wide scale adaptability and the like. The invention also provides a T cell product prepared by the method.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

The invention discloses a bionic heterogeneous tissue engineering meniscus stent and a preparation method thereof, and discloses application of the bionic heterogeneous tissue engineering meniscus stent in the field of meniscus repair. Based on a multi-nozzle 3D printing technology, a biodegradable high polymer material and bio-ink loaded with growth factors / stem cells are innovatively adopted, and the highly bionic heterogeneous tissue engineering meniscus is constructed. Specifically, degradable macromolecular polycaprolactone (PCL) is used as a raw material, and annular and radial fibers are printed to form a stent frame by accurately regulating and controlling a printing path and simulating a natural meniscus collagenous fiber distribution rule; on the basis, biological ink loaded with differentiated growth factors and stem cells is printed in a staggered manner according to different physiological characteristics of a red region and a white region of the meniscus, so that partitioned precise inoculation of seed cells and controllable partitioned release of the growth factors are realized, and a cell microenvironment with spatial specificity is provided for meniscus regeneration; the tissue repair efficiency and the bionic integrating degree are obviously improved.

The invention discloses a droplet micro-fluidic device for cell co-culture and a use method, belongs to the field of micro-fluidic analysis, and particularly relates to a droplet micro-fluidic device which comprises a chip main body material, an oily liquid substance covering the surface of the chip main body material, and a vertical porous membrane material arranged in the oily liquid substance. The porous membrane material comprises at least one of a polycarbonate porous membrane, a PVDF (Polyvinylidene Fluoride) porous membrane and a porous composite membrane, and the porosity of the porous membrane material is more than 1%. The droplet microfluidic device has the advantages of high flux, low consumption, multi-step operation, direct observation and the like, and has strong compatibility with a classical biochemical mechanism analysis method. The liquid drops are separated by the porous membrane material and are connected with each other, and the liquid drops on the two sides can be used as a cell, organoid or tissue culture chamber and also can be used as a liquid storage tank for sample adding. The liquid drops are used as a culture chamber, so that co-culture of cells can be realized, and support is provided for cell co-culture, drug screening and cell microenvironment simulation.

A theranostic composition and method for determining the cell-specific potency of drugs is provided. Further, compositions and methods useful for studying the therapeutic profile of one or more drugs within a cell microenvironment, including but not limited to a tumor, are provided.

The invention discloses application of a Dow Corning SYLGARD 184 organic silicon elastomer in preparation of a three-dimensional cell culture medium, and belongs to the technical field of biological materials. The matrix is formed by mixing an A-agent prepolymer and a B-agent curing agent of SYLGARD 184 according to the weight ratio of (8-12): 1 and then curing at the temperature of 35-39 DEG C. The method is characterized in that the substrate can support cell attachment without plasma treatment, and surface modification can be carried out through I-type collagen to enhance cell adhesion, so that a unique dual-mode cell microenvironment regulation and control system is formed. The matrix provided by the invention is simple to operate, low in cost and high in batch stability, effectively overcomes the defects of uncertain components, complex hydrogel synthesis treatment and the like of the existing animal-derived matrix, and can be widely applied to three-dimensional cell culture, disease model construction and drug screening platforms.

The present invention provides a cell laboratory observation system based on beam bundle light, which relates to the technical field of analysis and processing. It includes a non-linear beam self-fusion generator, a quantum entanglement state modulation network, a cell microenvironment response matrix, and a super-topological light field reconstruction unit for super-resolution observation and dynamic intervention of cells. Through the non-linear beam self-fusion technology, a self-focusing beam is generated by using a non-linear crystal doped with rare earth ions, and the focusing depth is dynamically adjusted by combining with a liquid crystal modulator, achieving sub-micron-level focusing without traditional lenses. The quantum entanglement state modulation network breaks through the optical diffraction limit through the non-local characteristics of entangled photon pairs. In addition, the chiral vortex beam generated by the super-topological light field reconstruction unit maintains high intensity and high resolution in deep tissues, enabling researchers to clearly observe fine structures such as neuron synapses or myocardial cell gap junctions in deep tissues, providing a high-precision tool for studying complex biological systems.

This invention discloses a method and system for predicting drug synergy based on cellular semantic-guided dynamic biological pathway weights. This method integrates semantic priors from a large language model, dynamic biological pathway gating, and a triplet self-attention mechanism. It dynamically regulates gene-pathway mapping relationships through cellular semantic features to form soft constraints, and applies biological hard constraints to network weights through a binary mask matrix constructed from gene-pathway subordination relationships. The combination of these two approaches, while adhering to the underlying facts of molecular biology, introduces semantic soft gating to adapt to the heterogeneity of the cellular microenvironment. On this basis, the complex interactions between drugs and cells are captured through a sequenced triplet self-attention mechanism, achieving accurate identification of drug synergy and deep coupling of cross-modal information, thereby improving the biological interpretability and generalization ability of the prediction.

A theranostic composition and method for determining the cell-specific potency of drugs is provided. Further, compositions and methods useful for studying the therapeutic profile of one or more drugs within a cell microenvironment, including but not limited to a tumor, are provided.

The invention discloses a blood vessel-cell microenvironment visualization model multi-parameter fusion analysis method based on the combination of DCE-MRI and DWI. The blood vessel-cell microenvironment visualization model multi-parameter fusion analysis method based on the combination of DCE-MRI and DWI comprises the following steps: constructing an LCBM blood vessel-cell microenvironment visualization model by quantifying parameters such as Ktrans, Vp and ADC; specifically, the research aims to clarify DCE-MRI / DWI characteristic spectrums of different tissue pathology subtypes of LCBM and reveal blood vessel-cell microenvironment characteristics of the LCBM, and the innovation of the research lies in that DCE-MRI blood vessel function parameters and DWI structure parameters are integrated for the first time, a blood vessel-cell microenvironment visualization model of the LCBM is constructed, and the blood vessel-cell microenvironment visualization model of the LCBM is established. An iconography basis is provided for precise typing of the LCBM and selection of an anti-vascular treatment target region, a new tool is provided for pathophysiology evaluation of the LCBM, and an iconography foundation is laid for an individualized treatment strategy targeting the pathophysiology of the LCBM.

The application provides a fluorescent probe for detecting cell microenvironment viscosity change and a preparation method thereof, and the fluorescent probe is 2-((E)-2-(6-((Z)-(4-(dimethylamino)phenyl)azo)-2,3-dihydro-1H-thioxanthene-4-yl)vinyl)-1,3,3-trimethyl-3H-indol-1-ium. The fluorescent probe can make the electron donor and the electron acceptor freely run around the N=N double bond in a low viscosity environment. When being stimulated by light, the energy of the excited state is released in a non-radiation mode through a TICT mechanism, so that weak fluorescence is displayed. In a high viscosity environment, intramolecular rotation is limited, the energy of the excited state is emitted in a light radiation mode, so that strong fluorescence is generated. The probe has high sensitivity for microenvironment viscosity detection and does not interfere with other common active oxygen, amino acid and enzyme species.

Described herein are systems and methods for intracellular imaging, assessment, and / or treatment of tissue before, during, and / or after surgical procedures using nanoparticles (e.g., less than 50 nanometers in diameter, e.g., photoswitchable nanoparticles) and / or a super-resolution microscope system. The present disclosure describes nanoparticles (e.g., nanosensors and photoswitchable nanoparticles) that are used to monitor and / or track changes in environmental conditions and / or analytes in the cellular microenvironment before, during, and / or after surgical procedures. The present disclosure also describes systems and methods that provide information related to the distribution and / or delivery of photoswitchable nanoparticles at super resolution (e.g., using super-resolution microscopy).

This invention discloses a method for preparing a ternary dynamic glycopeptide hydrogel bio-ink, comprising the following steps: dissolving methacrylamide gelatin, aldehyde-modified hyaluronic acid, hydrazide-modified gelatin, and a photoinitiator together in a solvent and mixing them evenly to obtain the bio-ink. This bio-ink constructs a dynamic covalent cross-linking network through reversible acylhydrazone bonds formed between aldehyde-modified hyaluronic acid and hydrazide-modified gelatin, providing a remodelable three-dimensional microenvironment for cells. Simultaneously, the photocrosslinking properties of methacrylic anhydride-modified gelatin are introduced to rapidly solidify and maintain overall shape stability during the printing stage, providing a remodelable cellular microenvironment during subsequent in vitro culture. This establishes an organoid culture platform with both good printability and excellent cell compatibility, providing a reliable material basis for subsequent drug screening and functional studies.

To provide a solution for quantitative measurement and monitoring of cellular mechanical forces that overcomes the shortcomings of existing technologies, requires no microscope, offers real-time, high-throughput, and low-cost capabilities, the inventors provide a cellular mechanical force detection device, comprising: a base, and a micropillar array consisting of multiple micropillars disposed on the base, capable of deformation under cellular mechanical forces. Each micropillar has a light-reflecting layer at its top or upper surface. The inventors also provide a cellular mechanical force detection system including the above-mentioned device, a detection method for detecting cellular mechanical forces using the above-mentioned device, and a method for preparing the above-mentioned device. Unlike existing technologies, the above-mentioned solution offers advantages such as high throughput and low cost, single-cell resolution, real-time monitoring, high sensitivity, and the ability to simulate the cellular microenvironment; it can also simulate the composition and morphology of the extracellular matrix, thus meeting a wider range of technical needs.

The invention provides a method for establishing a shrinking model for simulating uneven mixing of a large tank. The method comprises the following steps: detecting the mixing time of the large tank; and setting a reduction model parameter. The shrinking model established by the method disclosed by the invention can simulate the phenomenon of abnormal local osmotic pressure or pH rise caused by non-uniform mixing in the large tank, and comprehensively simulate the cell microenvironment in the large tank. Meanwhile, the method disclosed by the invention is simple in process, easy to implement and suitable for industrial popularization.

This invention discloses a fluorescent probe for detecting peroxynitrite in living cells and its synthesis method. The fluorescent probe comprises an amphiphilic group, 4-amino-2-methoxyphenol as a recognition unit, and a rhodamine derivative fluorophore, wherein the recognition unit can interact with ONOO. ‑ A specific oxidation reaction occurs, causing significant changes in fluorescence intensity and emission wavelength, thereby enabling real-time imaging and quantitative analysis of peroxynitrite in the cellular microenvironment. The fluorescent probe of this invention possesses high selectivity, strong anti-interference capabilities, and rapid response, making it suitable for the detection of ONOO at the live-cell level. ‑ Dynamic observation.

The application discloses a preparation method of a fluorescent probe capable of simultaneously detecting viscosity and polarity and application thereof in biological imaging and belongs to the technical field of chemical analysis and detection. The probe DHBP can simultaneously detect the changes of viscosity and polarity. With the increase of the solution viscosity, the fluorescence intensity of the red channel gradually increases, and the probe can be used as a viscosity sensitive probe. With the decrease of the solution polarity, the green channel of the probe is enhanced and the fluorescence of the red channel is enhanced, and the probe can be used as a polarity sensitive probe. The probe has low cytotoxicity, can be used for distinguishing normal cells and cancer cells, monitoring the inflammation induced by lipopolysaccharide and the changes of the cell microenvironment before and after treatment, and is successfully applied to the in vivo. In addition, the probe DHBP can evaluate the liver damage degree of a diabetic mouse and the drug treatment effect through fluorescence imaging.

A system for mimicking cellular microenvironment is provided and includes a chip, a hydrogel, and cells in contact with the hydrogel. The chip includes a first carrier having a pore and a second carrier coupled to the first carrier. The hydrogel is disposed in the pore and includes extracellular matrix. Also provided is a method for evaluating cell development, including providing the system for mimicking cellular microenvironment; providing a stimulation to a responsive hydrogel; and evaluating the cell development. Further provided is a method for evaluating drug therapy effectiveness, including providing the system for mimicking cellular microenvironment; administering the drug to the cells; and evaluating drug therapy effectiveness.

The invention belongs to the technical field of biological medicine, and particularly relates to application of farnesol in preparation of a medicine for resisting senescence of mesenchymal stem cells. The invention discloses farnesol as a traditional Chinese medicine monomer for the first time, which can effectively slow down the aging of adriamycin-induced adipose-derived stem cells in vitro, and can be used for preparing the anti-aging medicines for the mesenchymal stem cells. The farnesol can be used for removing aged adipose-derived stem cells and maintaining the steady state of a cell microenvironment. After treatment with 2 [mu] M farnesol for 24 h, key senescence markers such as P53 and P21 in adriamycin-induced senescence adipose-derived stem cells are significantly reduced, which indicates that farnesol can down-regulate senescence signal pathways in cells.

The application discloses a 3D bioprinted radial porous scaffold for staged bone regeneration and a preparation method thereof, the 3D bioprinted radial porous scaffold can provide a biomimetic cell microenvironment to support the infiltration of host repair cells to a defect site, and promote bone integration after implantation, in addition, the sequential release of double growth factors and the sustained release of PO4 3‑ and Ca 2+ in the 3D bioprinted radial porous scaffold can complete the three key steps of staged bone regeneration, can realize efficient bone regeneration, and represents a promising biomimetic staged regeneration strategy.

The invention discloses bionic hydrogel capable of being used for artificial organ orthotopic transplantation and application of the bionic hydrogel. Belongs to the technical field of medical materials. The bionic hydrogel comprises an adhesion hemostasis layer and a cell carrying layer, and the adhesion hemostasis layer is formed by polyvinyl alcohol (PVA) and boric acid and can rapidly stop bleeding and provide stable support for artificial organs; the cell carrying layer is composed of an acellular matrix, a phosphate buffer solution, gelatin and sodium alginate, can simulate the cell microenvironment of natural organs, and supports functional culture of organ parenchymal cells. According to the design, a dynamic cross-linked network is formed through chemical boric acid ester bonds, and excellent hemostatic performance, tissue adhesion, self-healing capacity and shape adaptability are achieved; meanwhile, mature organ function phenotypes are shown in vitro and can be quickly integrated with native organs, so that functions are exerted, and technical support is provided for human organ transplantation.

The invention belongs to the technical field of biological medicine and functional protein engineering, and particularly relates to recombinant IV type humanized collagen as well as preparation and application thereof. The invention provides a novel human-derived IV-type collagen functional fragment, which not only can be efficiently expressed and purified in a recombination mode, but also shows the capability of remarkably promoting human skin fibroblasts (HSF) to secrete and express III-type collagen in functional verification, and has definite tissue repair and skin regeneration regulation potential. In-vitro cell experiments prove that the recombinant collagen fragment provided by the invention can promote adhesion, migration and proliferation of fibroblasts or keratinocytes, and has the functions of repairing, regenerating and supporting a cell microenvironment.

This invention discloses a tumor cell hypoxia-sensitive fluorescent probe, its preparation method, and its application, belonging to the field of biomedical application technology. The tumor cell hypoxia-sensitive fluorescent probe provided by this invention uses triazine as the response unit, responding to the cellular microenvironment and being sensitive to hypoxia. It exhibits a significant specific activation effect in hypoxic tumor cells, enabling precise and efficient identification of hypoxic tumor cells.