10 results about "Cellular Microenvironment" patented technology

The invention discloses a method for low-density amplification of T cells and an obtained product, and belongs to the technical field of cell culture. The method comprises the following steps: activating a cell population containing T cells, and co-culturing with a lentiviral vector carrying a target gene for transduction; the method comprises the following steps: transduction is carried out on cells, then the transduction cells are inoculated into a shake flask at an inoculation density of not higher than 2 * 10 < 5 > cells / mL, shake culture is carried out to realize amplification, and amplification of 30-120 times can be realized. According to the method, a low-density cell microenvironment is optimized through dynamic oscillation, the problems of signal insufficiency, factor dilution and metabolism imbalance are solved, the efficiency bottleneck of traditional low-density amplification is broken through, and the method is suitable for large-scale production. The method has the beneficial effects of simplicity in operation, high cell product viability, high purity, low cost, wide scale adaptability and the like. The invention also provides a T cell product prepared by the method.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

This invention discloses a method and system for predicting drug synergy based on cellular semantic-guided dynamic biological pathway weights. This method integrates semantic priors from a large language model, dynamic biological pathway gating, and a triplet self-attention mechanism. It dynamically regulates gene-pathway mapping relationships through cellular semantic features to form soft constraints, and applies biological hard constraints to network weights through a binary mask matrix constructed from gene-pathway subordination relationships. The combination of these two approaches, while adhering to the underlying facts of molecular biology, introduces semantic soft gating to adapt to the heterogeneity of the cellular microenvironment. On this basis, the complex interactions between drugs and cells are captured through a sequenced triplet self-attention mechanism, achieving accurate identification of drug synergy and deep coupling of cross-modal information, thereby improving the biological interpretability and generalization ability of the prediction.

This invention discloses a method for preparing a ternary dynamic glycopeptide hydrogel bio-ink, comprising the following steps: dissolving methacrylamide gelatin, aldehyde-modified hyaluronic acid, hydrazide-modified gelatin, and a photoinitiator together in a solvent and mixing them evenly to obtain the bio-ink. This bio-ink constructs a dynamic covalent cross-linking network through reversible acylhydrazone bonds formed between aldehyde-modified hyaluronic acid and hydrazide-modified gelatin, providing a remodelable three-dimensional microenvironment for cells. Simultaneously, the photocrosslinking properties of methacrylic anhydride-modified gelatin are introduced to rapidly solidify and maintain overall shape stability during the printing stage, providing a remodelable cellular microenvironment during subsequent in vitro culture. This establishes an organoid culture platform with both good printability and excellent cell compatibility, providing a reliable material basis for subsequent drug screening and functional studies.

To provide a solution for quantitative measurement and monitoring of cellular mechanical forces that overcomes the shortcomings of existing technologies, requires no microscope, offers real-time, high-throughput, and low-cost capabilities, the inventors provide a cellular mechanical force detection device, comprising: a base, and a micropillar array consisting of multiple micropillars disposed on the base, capable of deformation under cellular mechanical forces. Each micropillar has a light-reflecting layer at its top or upper surface. The inventors also provide a cellular mechanical force detection system including the above-mentioned device, a detection method for detecting cellular mechanical forces using the above-mentioned device, and a method for preparing the above-mentioned device. Unlike existing technologies, the above-mentioned solution offers advantages such as high throughput and low cost, single-cell resolution, real-time monitoring, high sensitivity, and the ability to simulate the cellular microenvironment; it can also simulate the composition and morphology of the extracellular matrix, thus meeting a wider range of technical needs.

This invention discloses a fluorescent probe for detecting peroxynitrite in living cells and its synthesis method. The fluorescent probe comprises an amphiphilic group, 4-amino-2-methoxyphenol as a recognition unit, and a rhodamine derivative fluorophore, wherein the recognition unit can interact with ONOO. ‑ A specific oxidation reaction occurs, causing significant changes in fluorescence intensity and emission wavelength, thereby enabling real-time imaging and quantitative analysis of peroxynitrite in the cellular microenvironment. The fluorescent probe of this invention possesses high selectivity, strong anti-interference capabilities, and rapid response, making it suitable for the detection of ONOO at the live-cell level. ‑ Dynamic observation.

The invention belongs to the technical field of biological medicine, and particularly relates to application of farnesol in preparation of a medicine for resisting senescence of mesenchymal stem cells. The invention discloses farnesol as a traditional Chinese medicine monomer for the first time, which can effectively slow down the aging of adriamycin-induced adipose-derived stem cells in vitro, and can be used for preparing the anti-aging medicines for the mesenchymal stem cells. The farnesol can be used for removing aged adipose-derived stem cells and maintaining the steady state of a cell microenvironment. After treatment with 2 [mu] M farnesol for 24 h, key senescence markers such as P53 and P21 in adriamycin-induced senescence adipose-derived stem cells are significantly reduced, which indicates that farnesol can down-regulate senescence signal pathways in cells.

The application discloses a 3D bioprinted radial porous scaffold for staged bone regeneration and a preparation method thereof, the 3D bioprinted radial porous scaffold can provide a biomimetic cell microenvironment to support the infiltration of host repair cells to a defect site, and promote bone integration after implantation, in addition, the sequential release of double growth factors and the sustained release of PO4 3‑ and Ca 2+ in the 3D bioprinted radial porous scaffold can complete the three key steps of staged bone regeneration, can realize efficient bone regeneration, and represents a promising biomimetic staged regeneration strategy.

This invention discloses a tumor cell hypoxia-sensitive fluorescent probe, its preparation method, and its application, belonging to the field of biomedical application technology. The tumor cell hypoxia-sensitive fluorescent probe provided by this invention uses triazine as the response unit, responding to the cellular microenvironment and being sensitive to hypoxia. It exhibits a significant specific activation effect in hypoxic tumor cells, enabling precise and efficient identification of hypoxic tumor cells.

The present application relates to the technical field of skin care products, and in particular to a stem cell composition for improving aging and metabolic dullness and a preparation method thereof.The composition is compounded by an animal umbilical cord extract, a schizosaccharomyces pombe fermentation product lysate, DNA sodium, a tripeptide-105 and a cold orchid extract, and the five components synergistically regulate, accelerate melanin metabolism, reduce melanin deposition, inhibit melanin production, stimulate cell regeneration, replace old and worn-out cells, repair damaged cells, enhance cell vitality, inhibit inflammatory response, calm the cell microenvironment, and achieve the best long-acting lightening, whitening, barrier repair, soothing irritation, and improvement of severe acne clinical effects in terms of efficacy.The composition not only has faster and longer-lasting effects, but also has good safety and applicability, and has a broad market application prospect.