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A method for detecting the influence of cigarette smoke on the mRNA expression of aquaporin 5 cells

A technology of aquaporin and cigarette smoke, which is applied in the field of detection of the influence of cigarette smoke on the expression of aquaporin 5 cell mRNA, and can solve problems such as inapplicability to large-scale sample detection

Active Publication Date: 2021-02-02
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the effects of chemical substances on aquaporins in medicine are studied on the AQP5 at the whole animal level through the animal model of Sjogren's syndrome, which is not suitable for the detection of large quantities of samples

Method used

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  • A method for detecting the influence of cigarette smoke on the mRNA expression of aquaporin 5 cells
  • A method for detecting the influence of cigarette smoke on the mRNA expression of aquaporin 5 cells

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Experimental program
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Effect test

Embodiment 1

[0042]A method for detecting the effect of cigarette smoke on the mRNA expression of aquaporin 5 cells includes the following steps:

[0043]Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying the exposure of mainstream smoke to the oral cavity, using DMEM / F12 cell culture medium to infiltrate the inner wall of the simulated artificial oral mucosa of the device; select 10 weights Cigarettes consistent with the suction resistance are used as the sample to be tested, and after balancing, a fully automatic rotary smoking machine is used for suction. After the suction is finished, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; Equilibrium conditions are: temperature 21℃, humidity 58%, time 48h; the suction frequency of the suction is 1 mouth / min, the suction duration is 2s, and the suction capacity is 34.85mL / port;

[0044]In step (2), the salivary adenoid cystic carcinoma cells were ...

Embodiment 2

[0055]A method for detecting the effect of cigarette smoke on the mRNA expression of aquaporin 5 cells includes the following steps:

[0056]Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying the exposure of mainstream smoke to the oral cavity, using DMEM / F12 cell culture medium to infiltrate the inner wall of the simulated artificial oral mucosa of the device; select 20 weights Cigarettes consistent with the suction resistance are used as the sample to be tested, and after balancing, a fully automatic rotary smoking machine is used for suction. After the suction is finished, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; Equilibrium conditions are: temperature 23℃, humidity 62%, time 48h; the suction frequency of the suction is 1 mouth / min, the suction duration is 2s, and the suction capacity is 35mL±0.15mL / port;

[0057]In step (2), the salivary adenoid cystic carcinoma cells w...

Embodiment 3

[0070]A method for detecting the effect of cigarette smoke on the mRNA expression of aquaporin 5 cells includes the following steps:

[0071]Step (1), a biomimetic absorption device with patent application number 201520354840.8 for studying the exposure of mainstream smoke to the oral cavity was adopted, and 5mL DMEM / F12 cell culture medium was used to infiltrate the inner wall of the simulated artificial oral mucosa of the device; 15 pieces were selected Cigarettes with the same weight and suction resistance are used as the sample to be tested, and after balance, a fully automatic rotary smoking machine is used for suction. After the suction is finished, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; The equilibrium conditions are: temperature 22°C, humidity 60%, time 48h; the suction frequency of the suction is 1 port / min, the suction duration is 2s, and the suction capacity is 35 / port;

[0072]In step (2), the salivary adenoid...

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Abstract

The invention relates to a method for detecting the influence of cigarette smoke on the mRNA expression of aquaporin 5 cells, belonging to the technical field of biological applications. The present invention uses biochemical and cytological methods, uses BMP6 to treat cells, and simulates the cell microenvironment when Sjögren's syndrome occurs, so as to study the influence of cigarette smoke on the expression of AQP‑5, which is simpler than animal experiments , high efficiency; the oral bionic simulation device is used in the capture of cigarette smoke, which is more suitable for the actual human smoking state; at the same time, the fluorescence quantitative PCR method is used to compare the influence of different samples on the expression of AQP‑5 mRNA in real time , thus providing a new rapid method for evaluating the efficacy of cigarette smoke.

Description

Technical field[0001]The invention belongs to the technical field of biological applications, and specifically relates to a method for detecting the effect of cigarette smoke on the mRNA expression of aquaporin 5 cells.Background technique[0002]Saliva is the main factor in maintaining the balance of the oral microecological environment. It moisturizes the oral cavity and food, facilitates speech and swallowing, removes food particles from the taste buds, so as to continuously taste the taste of food and clean and protect the oral cavity. Cigarette sensory evaluation shows that some cigarette smoke can cause negative feelings such as dry mouth, dryness, discomfort, etc. Therefore, the development of cigarette products with the function of reducing dryness and promoting fluid is of great significance for improving oral comfort and improving the quality of cigarette smoking.[0003]Aquaporins (AQP) are a group of proteins that specifically transport water across membranes. They can signi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6851C12Q1/02C12N5/09
Inventor 高茜刘欣黄海涛向海英李雪梅孔维松杨光宇夭建华李晶
Owner CHINA TOBACCO YUNNAN IND
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