1076results about How to "Short experiment cycle" patented technology

The invention relates to a ginseng adventitious root induced proliferation method, which comprises the following steps: cutting ginseng tissue culture seedlings into small tissue blocks and then inoculating into a solid induced culture medium for inducing to form adventitious roots, cutting the adventitious roots into small adventitious root blocks and then inoculating into a liquid proliferation culture medium for performing proliferation culture of the adventitious roots, wherein the solid induced culture medium and the liquid proliferation culture medium take 1/2 MS(-N) culture medium as basic culture mediums and contain 1-10mg/L indolebutyric acid. By utilizing the method provided by the invention, the quantity of the adventitious roots obtained through induction can be obviously increased, the adventitious roots obtained through induction are subjected to proliferation culture, and compared with the method for performing adventitious root induction by utilizing callus tissues, the proliferation rate of the adventitious roots is higher, and the proliferation effect is better.

The invention provides a method for site-directed mutation of rice CENH3 gene by using a CRISPR-CAS9 technology. A CRISPR-CAS9-based sgRNA sequence is designed for the rice CENH3 gene, and a DNA fragment containing the sgRNA sequence is connected to a vector carrying the CRISPR/Cas to convert rice in order to realize the site-directed mutation of rice CENH3 gene, wherein the nucleotide sequence of the sgRNA action site is represented by SEQ ID NO:1. Rice endogenous gene CENH3 is edited through the CRISPR-CAS9 technology to obtain a CENH3 mutant, so a CENH3 mediated chromosome elimination mechanism is hopeful to be applied to the double haploid breeding process of rice.

The invention discloses a corona aging acceleration device and a corona aging acceleration method for a polymer insulating material. The device comprises a sealed corona aging experimental case in which a pin array electrode and a metal plate electrode are arranged, wherein the pin array electrode is connected with a high voltage generation device; the metal plate electrode is grounded; a pinpoint array is arranged on the pin array electrode; a test sample is arranged on the metal plate electrode, and is opposite to the pinpoint array; and after voltage is applied, uniform corona discharge is generated between the pin array electrode and the metal plate electrode to perform corona aging on the test sample. The device and the method have the advantages of low requirements on experimental conditions, convenience for operation and quantitative control over aging factors in an experiment, and short experimental period, and are applied to researches on the corona aging resistance of insulating materials in a laboratory.

The invention discloses a process of knocking out Wnt3a gene and a verification method thereof. The knockout and verification of Wnt3a gene are finished through the following steps: establishment of a Cas9 lentiviral vector for Wnt3a gene, culture and passage of HepG2 cell, lentivirus infection and screening of target cell, verification of gene knockout efficiency through a mispairing enzyme method, cell protein analysis and cell proliferation detection by a CCK-8 method. The invention has the following advantages: the Wnt3a gene is knocked out by establishing a Cas9 double-vector lentivirus system for the first time; Crispr/Cas9 is a technology for accurately editing specific site of the genome of any species, and the cell-level single gene or multiple genes can be knocked out by the technology; compared with other gene editing technologies, the method has the advantages that the targeting accuracy is higher; only if the RNA target sequence is completely matched with the genome sequence, can the Cas9 cut the DNA and realize simultaneous knockout of multiple sites of the target gene; and moreover, the experimental period of vector establishment is short, the time and the cost are remarkably saved, and species limit is avoided.

The invention provides a serum-free cell culture fluid suitable for enriching and culturing tumour stem cells. DMEM/f12 serves as the basic culture fluid of the serum-free cell culture fluid and transferrin, insulin and other substances are also added as the components of the serum-free cell culture fluid. The serum-free cell culture fluid provided by the invention can efficiently enrich tumour stem cells from malignant tumour cell strains and tumour tissues. In addition, the serum-free cell culture fluid can promote stable growth of the tumour stem cells and maintain fine cell activity and physiological properties of the tumour stem cells, thus being very suitable for research fields related to tumour cells and tumour stem cells.

The invention provides a method for site-directed mutation of a maize gene by means of a CRISPR/Cas9 system. An sgRNA sequence based on CRISPR/Cas9 is designed aiming at a target gene in maize, a DNA fragment for encoding the sgRNA sequence is connected to a vector carrying CRISPR/Cas9, and then maize transformation is conducted, so that site-directed mutation of a specific gene in maize is realized. Further, the vector containing CRISPR/Cas9 is transferred to an acceptor material carrying the target gene by means of a genetic transformation method, so as to obtain a site-directed mutation regeneration plant of the target gene. By the adoption of the method, site-directed mutation of a maize genome can be achieved. The method has the advantages that the experimental period is short, and operation is easy. Different target genes can be transformed in a site-directed mode by means of CRISPR/Cas9 systems with different targeting directions, a novel method is provided for improved breeding of maize, and the method is of great practical significance for maize trait improvement.

The invention relates to a preparation method of a single domain yttrium barium copper oxide superconductor; and the preparation method comprises the following steps: preparing Y2BaCuO5 precursor powder and liquid phase source powder, pressing a Y2BaCuO5 precursor block, a liquid phase block and a supporting bock, preparing a green body, growing a single domain yttrium barium copper oxide block in an infiltration manner, and carrying out oxygen permeation processing. A second phase nanoparticle Y2Ba4CuBiOx/Y2Ba4CuMOx (M is Bi or W) is successfully introduced for forming a flux pinning center by adopting a top seed crystal infiltration growing method and adding a metallic oxide (Bi2O3 powder and WO3 powder) for doping, thereby the powder preparation technology is simplified, the experimental period is shortened, the experiment cost is reduced, and the flux pinning capacity of the superconductor is increased. Y2O3 is used for preparing the supporting block which stably supports two briquettes above the supporting block in the slow cold growing process of an yttrium barium copper oxide block so as to prevent liquid phase from running off. The preparation method can be used for preparing the yttrium barium copper oxide superconductor and can also be used for preparing high-temperature superconductors of other series such as Gd, Sm, Nd, Eu and the like.

The invention relates to a method for preparing a pyramid array on a monocrystalline silicon substrate, and belongs to the technical field of manufacture of photovoltaic and semiconductor devices. The method comprises the following steps of: covering microballons in periodic arrangement on the surface of a monocrystalline silicon piece, and annealing near the glass transition temperature point of the microballoon; in oxygen atmosphere, obtaining a microballoon array in separation arrangement after etching by use of inductive coupling plasma; depositing a metallic titanium membrane on the monocrystalline silicon piece uniformly by a physical vapor deposition manner; and putting a silicon wafer with a masking film into an alkaline solution containing a surfactant for corrosion so as to obtain the pyramid array in order arrangement. The method is simple in process, short in preparation period and mature in technology; and three structures such as a positive pyramid array, an inverted pyramid array and a positive and inverted pyramid combined array can be obtained by a method for preparing a template through selecting and fine turning. The method has wide application value in the fields of photovoltaic, magnetic memory devices, nano photoelectric devices, nano sensors, surface raman enhancement and surface plasma effect and the like.

The invention discloses a constant-pressure concrete permeability testing device which comprises a permeameter, wherein the permeameter comprises a pressure water tank and a test-piece groove; the upper end of the pressure water tank is closed and provided with a pressure regulating valve and an air inlet valve; meanwhile, the air inlet valve is used as a water inlet valve; the lower end of the pressure water tank is open and closely connected with an opening in the upper end of the test-piece groove; an inner cavity of the test-piece groove penetrates through in an up-and-down through mode, the inner wall of the test-piece groove is provided with a partition cushion, the circumferential side as well as the edges of the upper and lower end surface of a test-piece to be tested are both attached to the partition cushion, and the test-piece groove can press against the partition cushion and the test-piece to be tested; a water containing container is arranged below the test-piece groove; the water containing container is placed on a balance. According to the device, a previous method for pressurizing through still water is changed, and the pressurizing is implemented by using an air compressor, so that an added pressure is effectively controlled and directly observed, and the test cycle is shortened. Seeped water is received by the water containing container and the electronic balance, the quantity of the seeped water is recorded in real time by using a computer, and a permeability coefficient is calculated in combination with the Darcy law. The device is good in sealing property, simple in operation easy to oeprate and high in testing accuracy.

The invention discloses a high-order rotating blade dynamic similarity test experiment table and a test method, and belongs to the technical field of the blade vibration test experiment. The experiment table disclosed by the invention comprises a base, a rotor driving part, a pneumatic shock excitation part and a data acquisition part, wherein the motor of the rotor driving part is connected with a spindle; a blade disc is sheathed on the spindle; the spindle is fixedly arranged on the base by a bearing pedestal; the total air duct of the pneumatic shock excitation part is communicated with a cylinder; a branch air duct air outlet on the cylinder corresponds to the blade disc; the first eddy current sensor of the data acquisition part is fixedly arranged on the base by a sensor bracket; and a second eddy current sensor is fixedly arranged on the base via a fixed mount. The test method for the high-order rotating blade dynamic similarity test experiment table comprises the following steps: selecting a prototype blade used for research; designing a model blade according to the relevant parameter theory of the prototype blade; manufacturing and processing the model blade used for the experiment; carrying out a static and dynamic kinetics characteristic test on the model blade; and according to a test result, predicting the kinetics characteristic of the prototype blade.

The invention discloses a making method of Li-La-Ti oxide in the lithium ion battery domain, which comprises the following steps: adopting lithium nitrate, lanthanum nitrate and butyl titanate as raw material with molecular formula as Li3xLa2/3-xTiO3 (0<x<0.16); setting the molar rate of Li, La and Ti at 1: 1: 2; adopting alcohol or ethylene glycol monomethyl ether as solvent; or adjusting pH value under 1 through water and acetate; drying solution; sintering under 800-900 deg.c for 2h; obtaining pure LLTO. the invention shortens experimental period and synthesizing temperature with grain size about 200nm, which possesses excellent compact and electric property.

The invention relates to a test device and a test method, which are used for researching the water erosion simulation of a slope channel system. The system comprises a bottom frame, and a slope runoff area and a channel runoff area which are connected with the bottom frame; one end of the bottom frame is connected with a travel wheel set, and the other end of the bottom frame is provided with a steering wheel set and a travel traction rod which is connected with the steering wheel set; the slope runoff area and the channel runoff area are both formed above the bottom frame; the end of the slope runoff area is hinged with the end of the channel runoff area; the other end of the channel runoff area is hinged with the travel wheel set end of the bottom frame; a channel lifting mechanism for controlling the channel runoff area to lift is arranged between the bottom frame and the channel runoff area; and a slope lifting mechanism for controlling the slope runoff area to lift is arranged between the bottom frame and the slope runoff area. The fine water erosion simulation test device has the advantages of manually tamping soil, transplanting plant, automatically adjusting the channel angles, freely moving the whole equipment, shortening a test period, and accurately simulating the water erosion process of soil under different site conditions.

The invention discloses a stable knockout single plasmid vector with coordination of transposon and a CRISPR/Cas9 system and application of stable knockout single plasmid vector and belongs to the field of gene engineering. The single plasmid vector is a double-stranded circular plasmid containing an IRDR-L-IRDR-R box, and the IRDR-L-IRDR-R box comprises an IRDR-L sequence, a promoter, a gRNA scaffold sequence, a Cas9 protein sequence, a resistance screening gene sequence and an IRDR-R sequence. The single plasmid vector provided by the invention can realize expression of sgRNA and a Cas9 protein only needing once construction and once transfection. A method is simple in process, is efficient and rapid, greatly simplifies a plasmid construction flow, shortens an experiment period and improves the working efficiency. The vector provided by the invention carries a transposase recognition sequence, does not use a virus, can conveniently, rapidly and safely establish a gene knockout stablecell line and is beneficial to screening stable cell lines by comprising the puromycin screening resistance.

The invention relates to an erosion-corrosion device, in particular to a jet type liquid-solid dual-phase fluid erosion-corrosion experimental device which solves the problems in the prior art that the adjustment of an attack angle is relatively difficult and the experimental period is long as well as the problem of real-time measurement of electrochemical parameters and the like. The experimental apparatus is provided with an ejector pump, a nozzle, a nozzle chamber and an electrochemical test system, and adopts the cam pump of a motor drive provided with a transducer as the ejector pump, wherein, an attack angle adjustment device used for adjusting the attack angle of samples is arranged in the nozzle chamber, the pipeline where the nozzle locates is connected with a jet distance adjustment device. The device adopts the cam pump as the ejector pump to realize the smooth conveying of media containing bigger solid phase particles, utilizes the cam pump of the motor drive provided with the transducer to control the flow speed; and the nozzle chamber is provided with the samples which can control the attack angle and the jet distance, the samples can fulfill the electrochemical measurement. The device can control the flow speed, attack angle and the temperature of the media, and fulfill the electrochemical measurement.

The invention discloses an experimental device for simulating water-salt movement of inland saline-alkali land and a method for screening different salt isolation measures and different coverage measures for preventing salt returning of the saline-alkali land by the experimental device. The experimental device comprises a soil column, a Marriotte bottle, an evaporation control box, a reverse filter layer and moisture, salt and temperature sensors. The soil column is filled with to-be-tested soil mass of the saline-alkali land, the Marriotte bottle is communicated with the lower portion of the soil column to supply water to the soil mass, the evaporation control box is mounted at the top end of the soil column, the reverse filter layer is arranged at the bottom of the soil column, and the moisture, the salt and the temperature sensors are mounted on side walls of the soil column. The experimental device is simple in structure and easy in operation and control of experimental conditions and can be used for laboratory simulation of various experiments such as influences of different measures on soil salinity movement of the saline-alkali land and influences of underground water level on water-salt movement of inland saline-alkali land, measurement results are accurate, and reliable basic data and technical support are provided for inland saline-alkali forestation.

The invention relates to a soil heavy metal migration and transformation simulating device. The device comprises a leaching column, a liquid storage system, multiple collection and detection units and a data acquisition and control system, variation of soil water potential in the leaching column is controlled through the liquid storage system, and thereby migration and transformation of heavy metal in soil are achieved. Leaching solutions in the leaching column are collected and detected through the collection and detection units, and leaching solution parameters and soil parameters can be acquired, stored and controlled through the data acquisition and control system. The simulating device integrates the functions of leaching solution storage, collection and detection and data acquisition, the structure is compact, the operation is convenient, a parameter optimization means is provided for establishing a migration and transformation model of heavy metal ions in soil, the test cycle is shortened, test precision is improved, a research result is more scientific and reliable, and the simulating device is a tool for conducting experimental research such as soil heavy metal ion migration.

The invention discloses a method and a device for determining saturation exponent of tight sandstone. The method comprises: according to nuclear magnetic resonance transverse relaxation time T2 spectrum of a core in a water saturation state and nuclear magnetic resonance transverse relaxation time T2 spectrum of cores in different water saturation states after different centrifugal forces are applied, determining geometric mean value of the nuclear magnetic resonance transverse relaxation time T2, the geometric mean value of the nuclear magnetic resonance transverse relaxation time T2 of the cores in different water saturation states after different centrifugal forces are applied on the cores, and water saturation of the cores after different centrifugal forces are applied; and according to the geometric mean value of the nuclear magnetic resonance transverse relaxation time T2 of the core in a water saturation state, and the geometric mean value of the nuclear magnetic resonance transverse relaxation time T2 of the cores in different water saturation states after different centrifugal forces are applied, and the water saturation of the cores after different centrifugal forces are applied, determining saturation exponent of the cores. The method and the device can accurately and efficiently determine the saturation exponent of tight sandstones.

The invention discloses a blisk dynamic similarity test bed and a test method. The blisk dynamic similarity test bed comprises a base, a test part and a data acquisition part. The test mainly comprises two parts of static natural frequency test and dynamic natural frequency test on a blisk structural part under a compressor system and a single blisk structural part. A compressor system test part is composed of a rotor driving part, a taper cylinder, a drum, a disk, labyrinth and the blisk and is fixed on a spindle through an expanded copling sleeve; according to the data acquisition part, a sensor is connected with an electric conduction link, the electric conduction link is fixed on the base through an electric conduction link support seat, an LMS test system is further connected, and in the case of the static test, an acceleration sensor can be directly connected onto the LMS test system. Static vibration test and dynamic vibration test on the blisk under a low-pressure compressor experiment system can be met, the experiment cost is reduced, and the experiment cycle is shortened.

The invention relates to an aqueous two-phase separation method for total flavonoids, saponins and polysaccharides in astragalus. The method comprises the following steps of smashing an astragalus medicinal material; adding a solvent and performing solid-liquid extraction; filtering and removing impurities to obtain crude extract of the total flavonoids and the saponins of the astragalus; taking and concentrating the crude extract of the astragalus; adding an aqueous two-phase system formed by absolute ethyl alcohol/dipotassium hydrogen phosphate, adding water to make the total amount of the system be a constant value; mixing uniformly and standing, wherein the aqueous two-phase system is divided into an upper phase and a lower phase; taking an ethanol phase which contains a large amount of total flavonoids and saponins of the astragalus out; and concentrating the ethanol phase under a reduced pressure to obtain the extract of the total flavonoids of the astragalus. The method has the beneficial effects that the extraction rate of the total flavonoids and the total saponins of the astragalus; simultaneously the total flavonoids and the total saponins can be separated from the polysaccharides of the astragalus in the crude extract; the used solvent is low in toxicity, favorable to environment friendliness and low in cost; the inactivation or the denaturation of the flavonoids and the saponins of the astragalus cannot be caused; the phase separation time is short; the interfacial tension is low; and residual organic solvent does not exist.

The invention provides a mechanical transmission gear knocking noise testing and noise quality quantitative evaluating method. The invention provides a detailed mechanical transmission gear knocking vibration noise testing method which comprises the steps of arranging a microphone and a vibration acceleration transducer; and quantitatively generating transmission gear knocking noise frequency characteristics in an idling condition and a creeping condition of a vehicle from a vibration acceleration signal and a noise signal which are measured in testing according to a spectrum analysis method, a time frequency analysis method and a coherent analysis method. According to the method of the invention, a unique objective evaluating method for the transmission gear knocking noise, namely a psychological parameter based on a language sharpness index is defined; a quantitative linear relation between a subjective annoyance performance value and a language sharpness index is generated; the determined quantitative relation between the subjective evaluation result and the objective evaluation result can be used for a plurality of subsequent tests repeatedly; and test period, manpower cost and physical resource cost can be greatly reduced.