8results about How to "Short experiment cycle" patented technology

The application discloses a device and method for preventing pollution and blockage of a cold stage system of a scanning electron microscope, the device comprising a main low-temperature gas path system and a normal-temperature nitrogen branch, low-temperature nitrogen discharged from the main low-temperature gas path system is divided into two paths, and is respectively connected to a cold hydrazine and a cold stage and is independently controlled through a flow valve. In order to solve the problems that a sample is easily polluted and a gas path is easily blocked during temperature rising in the prior art, a normal-temperature nitrogen branch is additionally arranged on the cold stage gas path through a three-way valve. During a system temperature rising stage, the cold stage low-temperature gas path is closed and the normal-temperature nitrogen branch is opened, dry nitrogen is blown to the cold stage, the cold stage is rapidly heated under positive pressure protection, meanwhile, the cold hydrazine continuously obtains low-temperature nitrogen, and an ultralow temperature is maintained. The above-mentioned measures ensure that a large temperature difference (such as > 150 DEG C) always exists between the cold hydrazine and the cold stage, ensure that pollutants are preferentially captured by the cold hydrazine, effectively protect the surface of the sample to be clean, and fundamentally prevent the gas path from being blocked due to moisture backflow.

The application discloses a retinal light damage box experimental device for observing mouse visual dynamic response, which comprises a reflecting box body, a box cover hinged to one side of the top end of the reflecting box body, the box cover being matched with the reflecting box body, a light irradiation assembly fixedly connected to one side of the box cover close to the inside of the reflecting box body, the inner wall of the reflecting box body being used for reflecting the light emitted by the light irradiation assembly, the light irradiation assembly being electrically connected with a control system, a frequency adjusting assembly fixedly connected to the middle of the bottom end of the reflecting box body, the frequency adjusting assembly being covered on a placing table, the frequency adjusting assembly being correspondingly arranged on the inner wall of the reflecting box body, and a placing assembly fixedly connected to the middle of the top end of the placing table, the experimental mouse being placed in the placing assembly, the light irradiation assembly and the frequency adjusting assembly being correspondingly arranged on the placing assembly. The application realizes dynamic observation of the light damage condition of the experimental animal, avoids repeated experiments, shortens the experimental period and improves the experimental efficiency.

This invention discloses a method and application for constructing personalized organoids for multiple myeloma based on stiffness gradient regulation, belonging to the field of biomedical technology. The method includes the following steps: S1: mixing patient bone marrow plasma with a calcium-containing crosslinking agent to form a gelatable precursor; S2: combining the gelatable precursor with a three-dimensional gelatin scaffold having a stiffness gradient (Young's modulus of 25-50 kPa) to construct a three-dimensional microenvironment simulating the stiffness gradient of bone marrow; S3: gelling the precursor to form a hydrogel-scaffold composite system encapsulating target cells; S4: seeding vascular endothelial cells onto the surface of the composite system to simulate a vascular niche structure. The organoid model for multiple myeloma constructed by this invention, based on the patient's autologous bone marrow plasma and possessing stiffness gradient characteristics, can effectively simulate the bone marrow mechanical microenvironment and preserve key components of the tumor microenvironment, supporting the long-term growth of primary tumor cells and improving the accuracy of drug sensitivity prediction.

This invention belongs to the interdisciplinary field of sensor design and artificial intelligence, specifically a sensor design method that proactively adapts to the application environment. This invention employs an active learning approach, actively selecting the most informative samples from unlabeled data for labeling, thereby maximizing model performance with less data acquisition. Compared to traditional methods of multiple sensor fabrications, active learning dynamically assesses the uncertainty or representativeness of data samples during model training, prioritizing data points that have the greatest impact on the model's decision boundaries and the strongest performance improvement potential. This method is not only applicable to a single type of sensor chip, but its database design and algorithm model are compatible with various material systems (including organic / inorganic, flexible / rigid, etc.), and can achieve rapid adaptive modeling and parameter recommendation for different application scenarios through transfer learning, demonstrating good scalability and versatility.

This invention belongs to the field of biomedical technology and discloses an in vitro evaluation method for GBM drug delivery systems based on tumor organoid chips. This method uses a PDMS tumor organoid chip as a carrier, co-culturing GBM cells and human umbilical vein endothelial cells to localize and culture GBM cells within the chip chamber. A biomimetic blood-brain barrier is constructed within the microchannels, and a perfusion system continuously maintains the cell growth microenvironment. After in vitro drug administration to the test drug delivery system, its anti-GBM effect is quantitatively detected from three dimensions: proliferation, invasion, and spheroidization. This invention can highly simulate the in vivo GBM tumor microenvironment and blood-brain barrier structure, achieving precise, real-time, and dynamic in vitro efficacy evaluation, while significantly reducing reliance on animal experiments, improving the reliability of results and experimental throughput. It provides an efficient and standardized platform for preclinical screening and mechanism of action research of anti-GBM drug delivery systems, and has significant scientific research value and clinical translational significance.

The application discloses a kind of thin film thermocouple line homogeneity test methods based on laser scanning heating, belong to thin film thermocouple field, comprising: the thin film thermocouple single branch to be tested is manufactured on insulating substrate, obtains thin film thermocouple branch;High-precision positioning laser is applied as transient heat source to the edge of thin film thermocouple branch, laser beam is moved and laser transient scanning is carried out along thin film thermocouple branch, local area of thin film thermocouple branch is heated, and non-steady temperature field is formed, and the thermoelectric output signal fluctuation data of the two ends of thin film thermocouple branch is collected;The homogeneity precision of thin film thermocouple branch is calculated using the data.The application adopts non-contact measurement, reduces external interference, ensures the accuracy and reliability of homogeneity test process;The application has high sensitivity and local response capability, and test process is fast and efficient, significantly shortens experimental period, reduces test cost, improves the efficiency of laboratory and industrial application simultaneously.

This invention provides a 3'-UTR element and its application in the production of target proteins. The 3'-UTR element comprises: (a) a nucleic acid molecule with a nucleotide sequence as shown in any of SEQ ID NO. 23, SEQ ID NO. 1-SEQ ID NO. 22, SEQ ID NO. 24-SEQ ID NO. 28, and SEQ ID NO. 37; (b) a nucleic acid molecule transcribed using the antisense strand of (a) as a template; or (c) a nucleic acid molecule derived from (a) or (b) by substitution, deletion, or addition of one or more nucleotides in the nucleotide sequence defined by (a) or (b) while retaining the biological function of its derived sequence. This element can stably and significantly enhance mRNA gene expression, providing a powerful tool for the development of mRNA vaccines and the optimization of gene expression in gene therapy.

This invention relates to the field of clinical virus detection, and more particularly to a protein dot blot detection kit, detection method, and application for varicella-zoster virus (VZV). The kit includes: a solid-phase carrier membrane for binding and immobilizing proteins in the sample; a blocking agent for blocking non-specific binding sites on the solid-phase carrier membrane; a first antibody that specifically recognizes the VZV antigen; an enzyme-labeled second antibody that binds to the first antibody; and a chromogenic solution for generating a detection signal. This kit is based on protein dot blot technology, detecting proteins with high specificity and a low false-positive rate. The experimental cycle is short, taking only 2-3 hours from sample pretreatment to signal detection, much faster than virus culture (requiring 4-7 days) and PCR detection (although fast, prone to false positives or false negatives), providing rapid evidence for clinical treatment, and is particularly suitable for the diagnosis of acute herpes zoster.