17results about How to "Short experiment cycle" patented technology

The invention provides a method for judging the inactivation temperature and the inactivation characteristic of a hydroformylation catalyst. The method comprises the following steps: S1, preparing a hydroformylation reaction solution; s2, measuring the reaction liquid, filling the reaction liquid into an adiabatic test instrument to simulate a hydroformylation reaction, and when the temperature is increased to T0, stopping heating and carrying out an adiabatic reaction; recording the temperature change, and obtaining the highest temperature Tm; s3, cooling the test ball, taking out half of a reaction material, adding a hydroformylation reaction raw material, heating to simulate a hydroformylation reaction, stopping heating when the temperature is increased to T1, carrying out an adiabatic reaction, and recording the highest temperature Tmf; s4, comparing T1, Tm and Tmf; if Tmf is equal to T1, the inactivation temperature of the catalyst is Tm, and irreversible inactivation is carried out; if Tmf is equal to Tm, the inactivation temperature of the catalyst is Tm, and reversible inactivation is carried out; if Tmfgt; and Tm, taking out the material in the test ball, and repeating the steps S2-S4. The judgment method disclosed by the invention is short in test period and high in safety, does not need separation and component analysis on a test sample, and has guiding significance on catalyst operation upper limit and homogeneous catalyst recovery temperature selection research.

The application discloses a kind of plant whole genome RNA-chromatin interaction detection methods, the method includes the interaction state of fixed RNA and chromatin, preparation double link, obtain nuclear, chromatin DNA fragment, RNA / linker / DNA chimera, RNA-DNA double-stranded complex in turn;Tn5 transposase fragmentization cuts double-stranded complex;PCR amplification forms DNA library.The application applies Tn5 transposase to the original RADICL-seq method, successfully to RNA-DNA complex is cut and library, greatly simplifies the process, experimental procedure is reduced by at least half, experimental period is shortened from 4-5 days to 3 days, the fragment size of DNA library produced is between 250bp-400bp, increase the length of final effective RNA / DNA fragment pair, thereby greatly improve the efficiency when aligning to plant genome in subsequent bioinformatics analysis, provide effective means for plant whole genome RNA-chromatin interaction detection.

The application discloses a device and method for preventing pollution and blockage of a cold stage system of a scanning electron microscope, the device comprising a main low-temperature gas path system and a normal-temperature nitrogen branch, low-temperature nitrogen discharged from the main low-temperature gas path system is divided into two paths, and is respectively connected to a cold hydrazine and a cold stage and is independently controlled through a flow valve. In order to solve the problems that a sample is easily polluted and a gas path is easily blocked during temperature rising in the prior art, a normal-temperature nitrogen branch is additionally arranged on the cold stage gas path through a three-way valve. During a system temperature rising stage, the cold stage low-temperature gas path is closed and the normal-temperature nitrogen branch is opened, dry nitrogen is blown to the cold stage, the cold stage is rapidly heated under positive pressure protection, meanwhile, the cold hydrazine continuously obtains low-temperature nitrogen, and an ultralow temperature is maintained. The above-mentioned measures ensure that a large temperature difference (such as > 150 DEG C) always exists between the cold hydrazine and the cold stage, ensure that pollutants are preferentially captured by the cold hydrazine, effectively protect the surface of the sample to be clean, and fundamentally prevent the gas path from being blocked due to moisture backflow.

The application discloses a retinal light damage box experimental device for observing mouse visual dynamic response, which comprises a reflecting box body, a box cover hinged to one side of the top end of the reflecting box body, the box cover being matched with the reflecting box body, a light irradiation assembly fixedly connected to one side of the box cover close to the inside of the reflecting box body, the inner wall of the reflecting box body being used for reflecting the light emitted by the light irradiation assembly, the light irradiation assembly being electrically connected with a control system, a frequency adjusting assembly fixedly connected to the middle of the bottom end of the reflecting box body, the frequency adjusting assembly being covered on a placing table, the frequency adjusting assembly being correspondingly arranged on the inner wall of the reflecting box body, and a placing assembly fixedly connected to the middle of the top end of the placing table, the experimental mouse being placed in the placing assembly, the light irradiation assembly and the frequency adjusting assembly being correspondingly arranged on the placing assembly. The application realizes dynamic observation of the light damage condition of the experimental animal, avoids repeated experiments, shortens the experimental period and improves the experimental efficiency.

This invention discloses a method and application for constructing personalized organoids for multiple myeloma based on stiffness gradient regulation, belonging to the field of biomedical technology. The method includes the following steps: S1: mixing patient bone marrow plasma with a calcium-containing crosslinking agent to form a gelatable precursor; S2: combining the gelatable precursor with a three-dimensional gelatin scaffold having a stiffness gradient (Young's modulus of 25-50 kPa) to construct a three-dimensional microenvironment simulating the stiffness gradient of bone marrow; S3: gelling the precursor to form a hydrogel-scaffold composite system encapsulating target cells; S4: seeding vascular endothelial cells onto the surface of the composite system to simulate a vascular niche structure. The organoid model for multiple myeloma constructed by this invention, based on the patient's autologous bone marrow plasma and possessing stiffness gradient characteristics, can effectively simulate the bone marrow mechanical microenvironment and preserve key components of the tumor microenvironment, supporting the long-term growth of primary tumor cells and improving the accuracy of drug sensitivity prediction.

The invention discloses a shale oil rock pore water determination method. The method comprises the following steps: cutting a frozen shale oil reservoir sample into a tested sample with the thickness of 10-20mm and the mass of 10-35g; the tested sample is placed in a vacuum container at the constant temperature of 60 DEG C for 8 h, the vacuum container is communicated with a liquid collector, and the liquid collector is connected with a freezing mechanism; liquid separated from the tested sample is frozen and recovered into the liquid collector, and the pore water volume in the collector is measured; the problems that the existing shale oil rock pore water determination needs a plurality of samples, the process is tedious, the working efficiency is low, errors are easily generated, and the accuracy of the determination result is influenced are effectively solved.

The utility model relates to an experimental condenser pipe automatic liquid supply device which comprises a condenser pipe, the condenser pipe is sleeved with a clamping assembly, a liquid supply sliding block is inserted into the right end of the clamping assembly and is connected with a sliding groove assembly in a sliding mode, and the lower portion of the sliding groove assembly is detachably connected with the upper portion of an anti-overflow box. The liquid supply sliding block and the sliding groove assembly are respectively provided with overflow liquid guide holes capable of guiding overflow liquid to the anti-overflow box. A first connector and a second connector are arranged at the top of the right end of the clamping assembly, and a liquid supply connector and a liquid drainage connector are arranged on the side face of the right end of the clamping assembly; the first joint is communicated with the liquid supply joint, the second joint is communicated with the liquid discharge joint, and the liquid inlet and the liquid outlet are respectively connected with the first joint and the second joint through pipelines; the liquid supply sliding block is provided with sliding block connecting pipes which are connected with the liquid supply connector and the liquid drainage connector in an inserted mode respectively.

The utility model discloses a steel plate crack simulation test device, which is characterized in that a chute is arranged on the bottom surface of an inner cavity of a concave test bench, a screw rod is positioned in the chute, a fixed block is arranged on one side of the inner cavity of the concave test bench, a driving motor is arranged in the fixed block, a driving shaft is connected with one end of the screw rod, and the other end of the screw rod is arranged on the other side of the inner cavity of the concave test bench; the movable block is located in an inner cavity of the concave test bench and is driven by the screw to move along the sliding groove, the fixed plate and the movable plate are connected through the positioning columns, the top face of the fixed plate and the top face of the movable plate are the same plane, and the limiting sliding strips are arranged on the side faces of the fixed plate and the movable plate to lock the moving distance of the movable plate relative to the fixed plate. And the laser extensometer is arranged on the other side of the inner cavity of the concave test bench and is used for measuring the moving distance of the movable plate. The device can quickly simulate the appearance and expansion process of cracks on the surface of the steel plate, so that the performance of the crack detection sensor is verified, the test result is accurate and reliable, the test period is greatly shortened, and the test cost is saved.

This invention belongs to the interdisciplinary field of sensor design and artificial intelligence, specifically a sensor design method that proactively adapts to the application environment. This invention employs an active learning approach, actively selecting the most informative samples from unlabeled data for labeling, thereby maximizing model performance with less data acquisition. Compared to traditional methods of multiple sensor fabrications, active learning dynamically assesses the uncertainty or representativeness of data samples during model training, prioritizing data points that have the greatest impact on the model's decision boundaries and the strongest performance improvement potential. This method is not only applicable to a single type of sensor chip, but its database design and algorithm model are compatible with various material systems (including organic / inorganic, flexible / rigid, etc.), and can achieve rapid adaptive modeling and parameter recommendation for different application scenarios through transfer learning, demonstrating good scalability and versatility.

The invention discloses a method for screening drought-resistant gene transgenic cotton in the technical field of biology, which comprises the following steps: step 1, setting an experimental group and a control group, the experimental group being a plurality of transgenic cotton seeds to be tested, and the control group being a plurality of common cotton seeds; 2, cotton physiological water demand is calculated, and a plurality of irrigation gradients are set; 3, determining the germination potential and germination rate of the cotton seeds of the experimental group and the control group, and transplanting normally germinated cotton seedlings; 4, irrigation control pots are prepared, cotton seedlings of the experimental group and the control group are planted in the irrigation control pots placed in the greenhouse, illumination and temperature are controlled to be consistent, and cotton plants of the experimental group and the control group are irrigated according to the irrigation gradient; 5, counting the survival rate of the cotton plants of the experimental group and the control group under different irrigation gradients, and controlling the boll setting number in the quality comparison group to be consistent when the cotton grows to the flowering and boll setting period; 6, picking the cotton, and detecting and comparing the yield and quality characters.

This invention belongs to the field of biomedical technology and discloses an in vitro evaluation method for GBM drug delivery systems based on tumor organoid chips. This method uses a PDMS tumor organoid chip as a carrier, co-culturing GBM cells and human umbilical vein endothelial cells to localize and culture GBM cells within the chip chamber. A biomimetic blood-brain barrier is constructed within the microchannels, and a perfusion system continuously maintains the cell growth microenvironment. After in vitro drug administration to the test drug delivery system, its anti-GBM effect is quantitatively detected from three dimensions: proliferation, invasion, and spheroidization. This invention can highly simulate the in vivo GBM tumor microenvironment and blood-brain barrier structure, achieving precise, real-time, and dynamic in vitro efficacy evaluation, while significantly reducing reliance on animal experiments, improving the reliability of results and experimental throughput. It provides an efficient and standardized platform for preclinical screening and mechanism of action research of anti-GBM drug delivery systems, and has significant scientific research value and clinical translational significance.

The present invention relates to a method for establishing a mouse model of Sjögren's syndrome. The mouse model is established by injecting an emulsion prepared by mixing thymus protein with Titermax adjuvant into the sole of a mouse, and then performing water intake detection, saliva volume detection, tear volume detection, corneal fluorescein staining detection, histopathological detection and serum immunological detection on the mouse model, thereby screening and obtaining a qualified mouse model. This method only requires one immunization to complete the construction of the model, with little secondary damage, a short experimental cycle and a high model success rate. At the same time, the model is comprehensively and reliably verified and evaluated for relevant characteristic changes based on both in vivo and in vitro levels, providing a new tool and platform for the disease research of Sjögren's syndrome.

The invention discloses a VSe2atCoFe2O4 composite wave-absorbing material and a preparation method thereof.The preparation method comprises the following steps that 1, cobalt chloride and ferric chloride are dissolved in deionized water, the PH is adjusted through a sodium hydroxide solution to be 12-13, then the solution is transferred into a high-pressure kettle, hydrothermal treatment is conducted for 20-24 h, after the reaction is finished, washing is conducted for multiple times through deionized water and ethyl alcohol, drying is conducted, and CoFe2O4 is obtained; (2) ammonium metavanadate and oxalic acid are dissolved in deionized water, selenium powder is added after dissolution, a certain amount of cobalt ferrite is added after uniform stirring, stirring and ultrasonic treatment are carried out for 1-2 h respectively, then the mixture is transferred into a high-pressure kettle, hydrothermal treatment is carried out for 20-24 h, centrifugation is carried out after the reaction is finished, washing is carried out multiple times with deionized water and ethyl alcohol, drying is carried out, and a series of VSe2CoFe2O4 composite wave-absorbing materials with different cobalt ferrite contents are obtained. The preparation method adopts a two-step hydrothermal method, the method is simple, the cost is lower, the experimental period is shorter, the reaction condition is mild, non-toxic raw materials are adopted, and the preparation process meets the industrial production of environment-friendly requirements.

The invention belongs to the technical field of gene engineering, and discloses a primer combination for detecting staphylococcus aureus and a drug-resistant gene and application of the primer combination, the primer combination comprises primer pairs which are respectively designed by taking a species-specific identification gene of the staphylococcus aureus and at least two antibiotic drug-resistant genes as target genes, the primer pair is composed of a common primer and a molecular beacon probe with primer and probe functions. When the primer combination is used as a primer of the RAA kit, the detection efficiency can be remarkably improved, and the amplification specificity and sensitivity are improved. The RAA method based on the primer combination can realize triple fluorescent quantitative detection, and pathogenic microorganisms and drug-resistant genes thereof can be detected at the same time. The method is suitable for normal-temperature amplification and detection of target genes.

The application discloses a kind of thin film thermocouple line homogeneity test methods based on laser scanning heating, belong to thin film thermocouple field, comprising: the thin film thermocouple single branch to be tested is manufactured on insulating substrate, obtains thin film thermocouple branch;High-precision positioning laser is applied as transient heat source to the edge of thin film thermocouple branch, laser beam is moved and laser transient scanning is carried out along thin film thermocouple branch, local area of thin film thermocouple branch is heated, and non-steady temperature field is formed, and the thermoelectric output signal fluctuation data of the two ends of thin film thermocouple branch is collected;The homogeneity precision of thin film thermocouple branch is calculated using the data.The application adopts non-contact measurement, reduces external interference, ensures the accuracy and reliability of homogeneity test process;The application has high sensitivity and local response capability, and test process is fast and efficient, significantly shortens experimental period, reduces test cost, improves the efficiency of laboratory and industrial application simultaneously.

This invention provides a 3'-UTR element and its application in the production of target proteins. The 3'-UTR element comprises: (a) a nucleic acid molecule with a nucleotide sequence as shown in any of SEQ ID NO. 23, SEQ ID NO. 1-SEQ ID NO. 22, SEQ ID NO. 24-SEQ ID NO. 28, and SEQ ID NO. 37; (b) a nucleic acid molecule transcribed using the antisense strand of (a) as a template; or (c) a nucleic acid molecule derived from (a) or (b) by substitution, deletion, or addition of one or more nucleotides in the nucleotide sequence defined by (a) or (b) while retaining the biological function of its derived sequence. This element can stably and significantly enhance mRNA gene expression, providing a powerful tool for the development of mRNA vaccines and the optimization of gene expression in gene therapy.

This invention relates to the field of clinical virus detection, and more particularly to a protein dot blot detection kit, detection method, and application for varicella-zoster virus (VZV). The kit includes: a solid-phase carrier membrane for binding and immobilizing proteins in the sample; a blocking agent for blocking non-specific binding sites on the solid-phase carrier membrane; a first antibody that specifically recognizes the VZV antigen; an enzyme-labeled second antibody that binds to the first antibody; and a chromogenic solution for generating a detection signal. This kit is based on protein dot blot technology, detecting proteins with high specificity and a low false-positive rate. The experimental cycle is short, taking only 2-3 hours from sample pretreatment to signal detection, much faster than virus culture (requiring 4-7 days) and PCR detection (although fast, prone to false positives or false negatives), providing rapid evidence for clinical treatment, and is particularly suitable for the diagnosis of acute herpes zoster.