4results about How to "High proliferation rate" patented technology

This invention discloses a method for preparing cell-derived exosome subsets, as well as products and applications, belonging to the field of biomedical technology. The preparation method includes: filtering cell culture medium to obtain a cell culture supernatant; concentrating the supernatant 5-30 times using a tangential flow system with a 300-500 kDa hollow fiber filter, followed by 10-20 times washing to obtain a concentrated solution; and performing Sephacryl S-400 HR gel filtration column chromatography, collecting the eluent from two 280 nm UV absorption peaks to obtain exosome subsets S1 and S2; the protein concentration of the concentrated solution is 20-50 mg / mL. This invention achieves, for the first time, precise separation of functional exosome subsets. Subset S1 exhibits strong immunomodulatory activity, and subset S2 demonstrates excellent cell regeneration promotion effects. Furthermore, the process is highly versatile and suitable for large-scale production. These subsets can be used to prepare immunomodulatory or cell regeneration-promoting drugs, providing precise technical solutions for the treatment of related diseases.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The invention belongs to the technical field of biomedical cosmetics, and discloses a double-target 5alpha-reductase and adiponectin receptor cyclic peptide as well as application and a preparation method thereof. The cyclic peptide is formed by cyclizing three amino acid units, namely arginine, phenylalanine and tyrosine through peptide bonds, and comprises pharmaceutically acceptable salts, solvates, stereoisomers or isotope labels of the cyclic peptide. The compound reduces generation of dihydrotestosterone by inhibiting 5alpha-reductase, activates an adiponectin receptor AdipoR pathway to promote energy metabolism and proliferation of hair papilla cells, and realizes double-target synergistic anti-alopecia. The invention also discloses application of the cyclopeptide in preparation of medicines or cosmetics for preventing or treating alopecia and a solid-phase synthesis preparation method. Through double-target synergistic interaction, the hair follicle cell proliferation rate can be remarkably increased at extremely low concentration, and the dosage is reduced compared with that of a traditional medicine; the composition is constructed by adopting physiological amino acid, has no systemic sex hormone side effect during local external use, and is high in safety.

The application discloses a liquid lithium-lead self-cooling cladding with low MHD pressure drop, and relates to the technical field of fusion reactor engineering; comprising: an outer cladding, which comprises an outer cladding circumferential arc-shaped main flow channel; the outer cladding circumferential arc-shaped main flow channel comprises a first wall region flow channel and a tritium breeding zone flow channel arranged in sequence from inside to outside along the radial direction; an inner cladding, which comprises an inner cladding circumferential arc-shaped main flow channel; wherein the flow direction of the outer cladding circumferential arc-shaped main flow channel and the inner cladding circumferential arc-shaped main flow channel is parallel or tends to be parallel to the magnetic line direction of the circumferential magnetic field of the fusion reactor; the outer cladding circumferential arc-shaped main flow channel and the inner cladding circumferential arc-shaped main flow channel are provided with flow channel inserts at the turning positions of the inlet and the outlet; and the polar height a of the outer cladding circumferential arc-shaped main flow channel and the inner cladding circumferential arc-shaped main flow channel is greater than the radial width b; the cladding has the advantages of low MHD pressure drop, simple structure, small processing difficulty and easy realization in engineering.