The invention relates to a selenium-rich microbial preparation prepared by fermentation of animal serum. The selenium-rich microbial preparation comprises the following raw materials in parts by weight: 8-15 parts of sodium selenite, 50-65 parts of animal blood hydrolysate, 5-15 parts of composite trace elements, 8-20 parts of potassium dihydrogen phosphate, 10-25 parts of urea, 8-20 parts of peptone, 4-10 parts of yeast powder, 8-20 parts of NaCl, 8-15 parts of NaOH, 4-10 parts of NaNO3, 10-25 parts of NaCl, 4-10 parts of NH4Cl, 8-20 parts of K2HPO4, 4-10 parts of beef extract, 10-25 parts of glucose, 10-25 parts of starch, 8-20 parts of calcium carbonate, 10-25 parts of amino acid, 2-6 parts of potassium nitrate, 2-6 parts of ferrous sulfate, 10-25 parts of magnesium sulfate, 25-40 parts of trisodium citrate, 10-25 parts of an biochemical enzyme and 800-848 parts of a mixed bacterial solution. According to the preparation method of the selenium-rich microbial preparation provided by the invention, the fermentation time is short, and the preservation period of the microbial agent can be prolonged to 2 years by increasing the proliferation speed of microbes in the fermentation process.