16results about How to "High proliferation rate" patented technology

The invention discloses an euphausia superba oligopeptide nutritional composition for promoting neural development and application thereof, and belongs to the technical field of functional foods.The nutritional composition is prepared from, by weight, 0.01-10 parts of docosahexaenoic acid, 0.01-20 parts of arachidonic acid, 0.01-10 parts of phosphatidylserine and 0.01-80 parts of euphausia superba oligopeptide. The antarctic krill oligopeptide is obtained by treating antarctic krill, antarctic krill powder or degreased antarctic krill powder by utilizing an enzymolysis method, and the content of a peptide fragment with the molecular weight of less than 1000Da in the antarctic krill oligopeptide is more than 90%. The raw materials of the composition are easy to obtain, the safety is high, and the composition can be used for preparing products with the function of promoting neurodevelopment and can be taken as common food, health care products or medicines for a long time.

The invention relates to an anti-aging composition containing recombinant collagen and a preparation method thereof. The main component of the composition is high-purity collagen obtained through a gene recombination technology, and various anti-aging active components such as sodium hyaluronate, vitamin C, coenzyme Q10 and vitamin E are combined. The invention also provides a preparation method, which ensures that each component is uniformly distributed and the biological activity is not damaged through low-temperature homogenization treatment and accurate proportioning. The composition disclosed by the invention can be used for remarkably promoting the proliferation of human skin fibroblasts and enhancing the expression of I-type collagen genes, and has a remarkable anti-aging effect. The composition is high in safety and remarkable in effect, is suitable for preventing and treating skin aging, and is widely applied to the fields of medicines and cosmetics.

This invention discloses a method for preparing cell-derived exosome subsets, as well as products and applications, belonging to the field of biomedical technology. The preparation method includes: filtering cell culture medium to obtain a cell culture supernatant; concentrating the supernatant 5-30 times using a tangential flow system with a 300-500 kDa hollow fiber filter, followed by 10-20 times washing to obtain a concentrated solution; and performing Sephacryl S-400 HR gel filtration column chromatography, collecting the eluent from two 280 nm UV absorption peaks to obtain exosome subsets S1 and S2; the protein concentration of the concentrated solution is 20-50 mg / mL. This invention achieves, for the first time, precise separation of functional exosome subsets. Subset S1 exhibits strong immunomodulatory activity, and subset S2 demonstrates excellent cell regeneration promotion effects. Furthermore, the process is highly versatile and suitable for large-scale production. These subsets can be used to prepare immunomodulatory or cell regeneration-promoting drugs, providing precise technical solutions for the treatment of related diseases.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The invention discloses an arrowroot tissue culture process which comprises the following steps: treating an explant, disinfecting, carrying out inoculated culture, cutting off a newly grown terminal bud when the terminal bud of the explant germinates and grows, removing the top of the terminal bud, inoculating the terminal bud into a starting induction culture medium to carry out adventitious bud induction, and removing useless parts of a block mass obtained in the previous step, so as to obtain the arrowroot. The method comprises the following steps of: inoculating the tissue culture seedlings obtained in the previous step into a proliferation and propagation culture medium for proliferation and propagation culture, removing useless parts of the tissue culture seedlings obtained in the previous step, inoculating the tissue culture seedlings into a hormone-reducing culture medium for hormone-reducing culture, then inoculating the tissue culture seedlings into a transition culture medium for transition culture, directly screening and planting the seedlings which reach the seedling standard, and performing rooting culture on the seedlings which do not reach the seedling standard. The method has the advantages that the proliferation rate can be greatly increased, the yellow leaf rate is reduced to 27.7%, the rooting rate is increased to 95.2%, and the seedling rate is increased to 91.6%. The productivity of the arrowroot is greatly improved, and great economic benefits are brought.

The invention provides a method for treating arthritis by using mesenchymal stem cells subjected to limited induction in vitro. Osteoarthritis is a common joint degenerative disease and is mainly characterized by cartilage injury and inflammatory response. The traditional treatment method is difficult to realize cartilage regeneration, but mesenchymal stem cells (MSC) show a prospect in joint repair due to multidirectional differentiation potential and immunomodulatory effect. According to the invention, bergenin (KGN) is utilized in vitro to induce MSC to differentiate into chondrocytes, so that the chondrogenic ability of the cells is enhanced. The inducible MSC is injected into a rat gonitis model, cartilage repair is remarkably promoted, and inflammation is reduced. Experimental results show that the method can effectively relieve arthrocele, improve the motion function and reduce the pain sensitivity. The invention not only improves the treatment effect of MSC, but also provides a safe and effective new approach for treating arthritis, and has important clinical application value.

The invention particularly discloses lepista sordida polysaccharide as well as a preparation method and application thereof. The lepista sordida polysaccharide comprises glucose and galactose, and the chemical structure of the lepista sordida polysaccharide comprises a 1, 4-linked D-glucose residue, a 1, 4-linked D-galactose residue, a 1, 6-linked galactose residue, a 2-linked alpha-D-glucose residue, a 1, 4, 6-linked glucose residue and a 1, 6-linked glucose residue. And the molar ratio of the residues of 1-> 4-Glc, 1-> 4-Gal, 1-> 6-Gal, 2->-Glc, 1-> 4, 6-Glc, and 1-> 6-Glc is about 3: 2: 3: 2: 2: 1. The lepista sordida polysaccharide is obtained through hot water extraction, ethanol precipitation, deproteinization, ion exchange column chromatography, dialysis and concentration. The lepista sordida polysaccharide disclosed by the invention has remarkable immunoregulatory activity and antitumor activity, and can be applied to medicines, health-care products or foods.

The invention relates to application of plant miRNA156a in preparation of a medicine for treating myocardial injury diseases, the miRNA156a is the plant miRNA156a, the plant is selected from salvia miltiorrhiza, liquorice, spinach, cabbage and broccoli, preferably, the miRNA156a is the salvia miltiorrhiza miRNA156a, the miRNA156a has the following nucleotide sequence: UGACAGAAGAGUGAGCAC (5 '-3'), and the miRNA156a can be modified by a chemical group, for example, the 3'end is modified by 2 '-O-CH3.

The invention belongs to the technical field of biomedical cosmetics, and discloses a double-target 5alpha-reductase and adiponectin receptor cyclic peptide as well as application and a preparation method thereof. The cyclic peptide is formed by cyclizing three amino acid units, namely arginine, phenylalanine and tyrosine through peptide bonds, and comprises pharmaceutically acceptable salts, solvates, stereoisomers or isotope labels of the cyclic peptide. The compound reduces generation of dihydrotestosterone by inhibiting 5alpha-reductase, activates an adiponectin receptor AdipoR pathway to promote energy metabolism and proliferation of hair papilla cells, and realizes double-target synergistic anti-alopecia. The invention also discloses application of the cyclopeptide in preparation of medicines or cosmetics for preventing or treating alopecia and a solid-phase synthesis preparation method. Through double-target synergistic interaction, the hair follicle cell proliferation rate can be remarkably increased at extremely low concentration, and the dosage is reduced compared with that of a traditional medicine; the composition is constructed by adopting physiological amino acid, has no systemic sex hormone side effect during local external use, and is high in safety.

The invention discloses magnesium phosphate bone cement with sonodynamic antibiosis / piezoelectric effect for stimulating cell proliferation. The magnesium phosphate bone cement is prepared from CaHPO4, NaH2PO4, MgO powder, hydroxyapatite and a heterojunction containing BOS. According to the invention, NTO is modified through ion doping and heterojunction construction, a nano material with both sonodynamic performance and piezoelectric performance is prepared, the nano material and MPC are compounded, and the modified magnesium phosphate bone cement with sonodynamic antibacterial / piezoelectric effect stimulated cell proliferation is prepared.

The invention relates to the technical field of stem cell preparation and culture, and discloses a hospital-grade olfactory mucosa mesenchymal stem cell preparation and sterile culture process which comprises the following steps: collecting olfactory mucosa tissues from a donor olfactory mucosa area through a minimally invasive surgery, putting the collected tissues into a cold-chain transportation solution, and transporting at the temperature of 4 DEG C or below; a device for hospital-level olfactory mucosa mesenchymal stem cell preparation and sterile culture comprises a closed sterile bioreactor system used for controlling temperature, CO2 concentration, O2 concentration and pH value. Through electrochemical signal regulation and control, a real-time monitoring system and A I intelligent feedback, accurate control over cell proliferation, differentiation and anti-inflammatory functions is achieved, electric field parameters are optimized at different stages, cell proliferation, neural differentiation and anti-inflammatory capacity is improved, and through real-time monitoring and automatic feedback, it is ensured that the culture process is efficient, accurate and automatic, and the culture efficiency is improved. The cell culture success rate is obviously improved.

The present invention relates to a culture medium, and in particular to a culture medium for improving the function of CAR-T cells and its application. The culture medium for improving the function of CAR-T cells is composed of a basal culture medium and the following components added to the basal culture medium: ergobenzyl ester, IL-2, IL-15, basic fibroblast growth factor, ethinyl estradiol, transferrin, and vitamin C. The addition of ergobenzyl ester and ethinyl estradiol can effectively improve the survival rate and proliferation rate of CAR-T cells; it can also effectively increase the proportion of central memory T cells of CAR-T cells after culture, thereby improving the efficiency and durability of CAR-T cell therapy. In addition, after culturing CAR-T cells with the culture medium of the present invention, they have a strong specific killing effect on tumor cells in vitro.

The application discloses a liquid lithium-lead self-cooling cladding with low MHD pressure drop, and relates to the technical field of fusion reactor engineering; comprising: an outer cladding, which comprises an outer cladding circumferential arc-shaped main flow channel; the outer cladding circumferential arc-shaped main flow channel comprises a first wall region flow channel and a tritium breeding zone flow channel arranged in sequence from inside to outside along the radial direction; an inner cladding, which comprises an inner cladding circumferential arc-shaped main flow channel; wherein the flow direction of the outer cladding circumferential arc-shaped main flow channel and the inner cladding circumferential arc-shaped main flow channel is parallel or tends to be parallel to the magnetic line direction of the circumferential magnetic field of the fusion reactor; the outer cladding circumferential arc-shaped main flow channel and the inner cladding circumferential arc-shaped main flow channel are provided with flow channel inserts at the turning positions of the inlet and the outlet; and the polar height a of the outer cladding circumferential arc-shaped main flow channel and the inner cladding circumferential arc-shaped main flow channel is greater than the radial width b; the cladding has the advantages of low MHD pressure drop, simple structure, small processing difficulty and easy realization in engineering.

The invention provides a 293T cell strain for expressing Claudin18.2 and a preparation method of the 293T cell strain. The preparation method comprises the following steps: packaging lentivirus by using a lentiviral vector for expressing Claudin18.2, and transfecting cells to obtain a virus solution; infecting 293T cells with the virus liquid according to the MOI of 0.1-1, culturing, and screening monoclone. The lentiviral vector for expressing the Claudin18.2 comprises an expression box for expressing the Claudin18.2, and a promoter of the expression box is CMV (Cytomegalovirus) and does not comprise a co-expression gene. The stably transfected cell strain obtained by the invention does not need to depend on drug screening, and can still stably express the Claudin18.2 gene and keep a good cell state after passage for more than 10 times. The cell strain can be used as a Claudin18.2 targeted monoclonal antibody, a bispecific antibody, an antibody coupling drug and an in-vitro killing experiment positive target cell of a chimeric antigen receptor T cell, an effective cell model is constructed for in-vitro functional verification of a targeted drug, and a reliable basis is provided for evaluating the killing efficiency.

The invention relates to the technical field of cell culture, in particular to a culture method for improving the durability of a CAR-T cell population, the culture method is characterized in that CAR-T cells are cultured by a CAR-T cell culture medium, and the CAR-T cell culture medium takes an LONZA X-VIVO15 serum-free culture medium as a basic component, the liquid medicine also comprises the following components: 2 g / L of cell factor microcapsules, 4 g / L of a fermentation compound, 20 mg / L of dextran sulfate, 2 mg / mL of vitamin C, 15 mM of 4-hydroxyethylpiperazine ethanesulfonic acid and 1.75 mM of amino acid. According to the CAR-T cell culture medium, all the components synergistically play a role and jointly provide a CAR-T cell nutrition matrix, and a tumor killing cell population with higher cell proliferation capacity and higher killing activity is obtained, so that the cultured CAR-T cells are high in proliferation rate and high in tumor killing activity, the curative effect is improved, and the durability is effectively improved.

The invention relates to the technical field of biological products, in particular to a preparation method of a culture medium for efficiently culturing mycoplasma gallisepticum at low cost, which comprises the following steps: step 1, dissolving and blending: a, preliminary dissolving: adding NaCl, K2HPO4 and MgSO4. 7H2O into 1000mL of distilled water, heating to 40 DEG C, and slightly stirring until the NaCl, K2HPO4 and MgSO4. 7H2O are completely dissolved to form a basic inorganic salt solution; b, adding a carbon and nitrogen source: adding glucose, soy peptone, tryptone and a yeast extract, and stirring until the materials are completely dissolved; c, pH regulation: regulating the pH value of the solution to 7.4 by using sterile 0.5 M HCl and NaOH; step 2, adding growth factors and nutritional ingredients: a, adding special growth factors: adding L-cysteine and cholesterol under a stirring condition, and continuously stirring until the L-cysteine and the cholesterol are completely dissolved; the cost is low: animal serum and protein raw materials are replaced by using the plant-derived protein hydrolysate, and meanwhile, auxiliary components such as vitamins and growth factors are added, so that the cost of the raw materials is reduced.